Protein: Chromosomal replication initiator protein DnaA (P05648)
Organism: Bacillus subtilis (strain 168)
Function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'- TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA can inhibit its own gene expression as well as that of other genes
Subcellular location: Cytoplasm
InterPro domains:
- IPR010921: Trp repressor/replication initiator (homologous_superfamily) [342-446]
- IPR001957: Chromosomal replication control, initiator DnaA (family) [5-446]
- IPR003593: AAA+ ATPase domain (domain) [143-271]
- IPR024633: DnaA N-terminal domain (domain) [7-65]
- IPR038454: DnaA, N-terminal domain superfamily (homologous_superfamily) [1-82]
- IPR013317: Chromosomal replication initiator protein DnaA, ATPAse domain (domain) [111-272]
- IPR018312: Chromosomal replication control, initiator DnaA, conserved site (conserved_site) [405-424]
- IPR013159: Chromosomal replication initiator, DnaA C-terminal (domain) [355-445]
- IPR020591: Chromosomal replication control, initiator DnaA-like (family) [144-424]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [93-323]
Sequence:
MENILDLWNQALAQIEKKLSKPSFETWMKSTKAHSLQGDTLTITAPNEFARDWLESRYLHLIADTIYELTGEELSIKFVIPQNQDVEDFMPKPQVKKAVKEDTSDFPQNMLNPKYTFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAIGHYVIDHNPSAKVVYLSSEKFTNEFINSIRDNKAVDFRNRYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNTLHEESKQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAEGLDIPNEVMLYIANQIDSNIRELEGALIRVVAYSSLINKDINADLAAEALKDIIPSSKPKVITIKEIQRVVGQQFNIKLEDFKAKKRTKSVAFPRQIAMYLSREMTDSSLPKIGEEFGGRDHTTVIHAHEKISKLLADDEQLQQHVKEIKEQLK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006260 (DNA replication), GO:0006270 (DNA replication initiation), GO:0006261 (DNA-templated DNA replication)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0032993 (protein-DNA complex), GO:0016020 (membrane), GO:0005657 (replication fork), GO:0030894 (replisome), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome)