Protein: Bile acid--coenzyme A ligase (P19409)
Organism: Clostridium scindens (strain JCM 10418 / VPI 12708)
Function: Functions in the bile acid 7alpha-dehydroxylation pathway, which forms secondary bile acids via the 7alpha-dehydroxylation of primary bile acids, and is carried out by intestinal anaerobic bacteria. Catalyzes the initial step in this pathway, i.e. the ATP- dependent thioesterification of primary bile acids with coenzyme A. Is active with C-24 bile acids with free carboxyl groups such as cholate, deoxycholate and chenodeoxycholate. Produces AMP and pyrophosphate in addition to the bile acid-CoA thioester
InterPro domains:
- IPR025110: AMP-binding enzyme, C-terminal domain (domain) [432-505]
- IPR042099: ANL, N-terminal domain (homologous_superfamily) [16-415]
- IPR000873: AMP-dependent synthetase/ligase domain (domain) [40-375]
- IPR050237: ATP-dependent AMP-binding enzyme (family) [233-515]
- IPR045851: AMP-binding enzyme, C-terminal domain superfamily (homologous_superfamily) [418-517]
- IPR054986: Bile acid--coenzyme A ligase (family) [16-518]
Sequence:
MHKKSACEREGKELKRDFFNKFNLGTSNFVTPGKQLEYVSECKPDSTAVICLDKEQNCSVITWHQLHVYSSQLAWYLIENEIGPGSIVLTMFPNSIEHIIAVFAIWKAGACYMPMSYKAAESEIREACDTIHPNAAFAECKIPGLKFCLSADEIYEAMEGRSKEMPSDRLANPNMISLSGGTSGKMKFIRQNLPCGLDDETIRSWSLMSGMGFEQRQLLVGPLFHGAPHSAAFNGLFMGNTLVLTRNLCPGNILNMIKKYKIEFIQMVPTLMNRLAKLEGVGKEDFASLKALCHTGGVCSPWLKQIWIDLLGPEKIYEMYSMTECIGLTCIRGDEWVKHPGSIGRPVGDSKVSIRDENGKEVAPFEIGEIYMTAPASYLVTEYINWEPLEVKEGGFRSVGDIGYVDEQGYLYFSDRRSDMLVSGGENVFATEVETALLRYKDILDAVVVGIPDEDLGRRLHAVIETGKEIPAEELKTFLRKYLTPYKIPKTFEFVRSIRRGDNGKADRKRILEDCIARGG
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0016877 (ligase activity, forming carbon-sulfur bonds), GO:0016878 (acid-thiol ligase activity), GO:0016405 (CoA-ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0016999 (antibiotic metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:0017000 (antibiotic biosynthetic process), GO:0042180 (cellular ketone metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:0044598 (doxorubicin metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:0044597 (daunorubicin metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0030638 (polyketide metabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:1902644 (tertiary alcohol metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0034308 (primary alcohol metabolic process), GO:0042181 (ketone biosynthetic process), GO:1901661 (quinone metabolic process), GO:1901657 (glycosyl compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0030648 (aminoglycoside antibiotic biosynthetic process), GO:0030639 (polyketide biosynthetic process), GO:1901659 (glycosyl compound biosynthetic process), GO:0016137 (glycoside metabolic process), GO:1901663 (quinone biosynthetic process), GO:0030647 (aminoglycoside antibiotic metabolic process), GO:0016138 (glycoside biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)