Protein: Oocyte-specific homeobox protein 3 (Q3UT54)
Organism: Mus musculus (Mouse)
Function: Transcription factor required for zygotic genome activation (ZGA), a critical event in early embryonic development during which the developmental control passes from maternally provided mRNAs to the expression of the zygotic genome after fertilization. Together with other Obox family members, required in early two-cell stage embryos to kick-start the major ZGA wave by facilitating RNA Polymerase II 'pre-configuration', during which RNA Polymerase II relocates from the initial one-cell stage binding targets to ZGA gene promoters and distal enhancers. Mechanistically, promotes recruitment of RNA Polymerase II from (CG-rich) non-ZGA genes to (CG-poor) ZGA genes at the two-cell stage. Binds to regulatory DNA sequences containing a 5'-ACNCCTTTAATCCCAG-3' sequence motif. Most maternal and zygotic Obox family proteins can compensate for one another
Subcellular location: Nucleus
InterPro domains:
- IPR050460: Distal-less homeobox transcription factors (family) [34-226]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [77-153]
- IPR001356: Homeodomain (domain) [92-156]
- IPR017970: Homeobox, conserved site (conserved_site) [127-150]
Sequence:
MAEGPSLHPKLQVASNIPIELSSQIPQDPARNLAFQMCQNPLMTPGSTMQSRLSVPERNLLQQESQGPTRQSGRMPLSDRYVNKQTGPMALRKFRKERTVYSKEQQCLLQKHFDEFQYPNEKKIVELALSVGVTKREIKIWFKNNRAKYRRMKLQNIKQALPETNESSKAVSESTHFPGSIPVVDSDNGESMCSGTFGEDSIPKLNCSQESSLHCFQACDGDMCCPQEYQEYQEYLLHGHAPVTAWNSGQSAAFESQTDLAVAEVPVGLAYAAQAPEDAHNSGPCEDELWQRILEDLDTSDDWLTLRNLSTPVYTTEVLDQSKPYSHEEVCYTHL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:1901363 (heterocyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0001216 (DNA-binding transcription activator activity), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0001228 (DNA-binding transcription activator activity, RNA polymerase II-specific), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003677 (DNA binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding), GO:0000987 (cis-regulatory region sequence-specific DNA binding), GO:0000977 (RNA polymerase II transcription regulatory region sequence-specific DNA binding), GO:0000978 (RNA polymerase II cis-regulatory region sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0019222 (regulation of metabolic process), GO:0008283 (cell population proliferation), GO:0051239 (regulation of multicellular organismal process), GO:0050793 (regulation of developmental process), GO:0048522 (positive regulation of cellular process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051093 (negative regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0008285 (negative regulation of cell population proliferation), GO:0048513 (animal organ development), GO:0060255 (regulation of macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0035295 (tube development), GO:0045165 (cell fate commitment), GO:0001709 (cell fate determination), GO:2000026 (regulation of multicellular organismal development), GO:0042127 (regulation of cell population proliferation), GO:0009888 (tissue development), GO:0031325 (positive regulation of cellular metabolic process), GO:0045596 (negative regulation of cell differentiation), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0008284 (positive regulation of cell population proliferation), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0035239 (tube morphogenesis), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0048663 (neuron fate commitment), GO:0060284 (regulation of cell development), GO:0048732 (gland development), GO:0051960 (regulation of nervous system development), GO:0045685 (regulation of glial cell differentiation), GO:0010468 (regulation of gene expression), GO:0030855 (epithelial cell differentiation), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0045668 (negative regulation of osteoblast differentiation), GO:0031326 (regulation of cellular biosynthetic process), GO:0030857 (negative regulation of epithelial cell differentiation), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0051252 (regulation of RNA metabolic process), GO:0060429 (epithelium development), GO:0045667 (regulation of osteoblast differentiation), GO:0022612 (gland morphogenesis), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0030856 (regulation of epithelial cell differentiation), GO:0007431 (salivary gland development), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0002065 (columnar/cuboidal epithelial cell differentiation), GO:0060443 (mammary gland morphogenesis), GO:0014015 (positive regulation of gliogenesis), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:0014013 (regulation of gliogenesis), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)