Protein: Bile acid CoA-transferase BaiF (P19413)
Organism: Clostridium scindens (strain JCM 10418 / VPI 12708)
Function: Functions in the bile acid 7alpha-dehydroxylation pathway, which forms secondary bile acids via the 7alpha-dehydroxylation of primary bile acids, and is carried out by intestinal anaerobic bacteria. Acts as a bile acid CoA transferase with broad bile acid substrate specificity. Catalyzes the transfer of the CoA moiety of secondary bile acid-CoA compounds to primary bile acids. Can use lithocholoyl-CoA, deoxycholoyl-CoA and allodeoxycholoyl-CoA as bile acid CoA donors and cholate, allocholate, chenodeoxycholate, ursodeoxycholate, and beta-muricholate as bile acid CoA acceptors . Also displays CoA hydrolase activity, being able to catalyze the hydrolysis of choloyl-CoA, 3-dehydrocholoyl-CoA, and chenodeoxycholoyl-CoA, releasing CoA and the corresponding free bile acid. However, this latter activity may not represent the actual activity of this enzyme, since using a transferase rather than hydrolase, the bacteria conserve the thioester bond energy, saving ATP molecules (Probable). Shows no hydrolytic activity with acetyl-CoA, isovaleryl-CoA, palmitoyl-CoA, or phenylacetyl-CoA as substrates
InterPro domains:
- IPR050509: Coenzyme A-transferase family III (family) [12-400]
- IPR023606: CoA-transferase family III domain 1 superfamily (homologous_superfamily) [8-400]
- IPR003673: CoA-transferase family III (family) [13-377]
- IPR044855: CoA-transferase family III domain 3 superfamily (homologous_superfamily) [226-329]
Sequence:
MAGIKDFPKFGALAGLKILDSGSNIAGPLGGGLLAECGATVIHFEGPKKPDNQRGWYGYPQNHRNQLSMVADIKSEEGRKIFLDLIKWADIWVESSKGGQYDRLGLSDEVIWEVNPKIAIVHVSGYGQTGDPSYVTRASYDAVGQAFSGYMSLNGTTEALKINPYLSDFVCGLTTCWAMLACYVSTILTGKGESVDVAQYEALARIMDGRMIQYATDGVKMPRTGNKDAQAALFSFYTCKDGRTIFIGMTGAEVCKRGFPIIGLPVPGTGDPDFPEGFTGWMIYTPVGQRMEKAMEKYVSEHTMEEVEAEMQAHQIPCQRVYELEDCLNDPHWKARGTITEWDDPMMGHITGLGLINKFKRNPSEIWRGAPLFGMDNRDILKDLGYDDAKIDELYEQGIVNEFDLDTTIKRYRLDEVIPHMRKKEE
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016782 (transferase activity, transferring sulphur-containing groups), GO:0008410 (CoA-transferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0016999 (antibiotic metabolic process), GO:0044281 (small molecule metabolic process), GO:0017000 (antibiotic biosynthetic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006066 (alcohol metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044283 (small molecule biosynthetic process), GO:1901135 (carbohydrate derivative metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:1901657 (glycosyl compound metabolic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0030648 (aminoglycoside antibiotic biosynthetic process), GO:0046165 (alcohol biosynthetic process), GO:0019751 (polyol metabolic process), GO:0046173 (polyol biosynthetic process), GO:1901659 (glycosyl compound biosynthetic process), GO:0016137 (glycoside metabolic process), GO:0030647 (aminoglycoside antibiotic metabolic process), GO:0016138 (glycoside biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)