Protein: Reverse gyrase (O29238)
Organism: Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
Function: Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Very efficient supercoiling occurs on relaxed DNA with a single- stranded bubble; the minimal bubble is 20 nucleotides (nt) and up to 10 positive supercoils can be introduced into a 3.1 kb plasmid with a 50 nt bubble. Positively supercoils DNA with all (d)NTPS, although it requires about 10-fold more of non-(d)ATP . In the absence of ATP (or at low levels of enzyme), or in the presence of ADP, relaxes negative supercoils . Only relaxes positive supercoils when the substrate contains a bubble . Also promotes strand annealing of complementary ssDNA circles. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection (Probable)
Subcellular location: Cytoplasm
InterPro domains:
- IPR040569: Reverse gyrase, zinc finger (domain) [1-43]
- IPR013826: DNA topoisomerase, type IA, central region, subdomain 3 (homologous_superfamily) [772-890]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [53-252]
- IPR003602: DNA topoisomerase, type IA, DNA-binding domain (domain) [762-1006]
- IPR013824: DNA topoisomerase, type IA, central region, subdomain 1 (homologous_superfamily) [680-1049]
- IPR034142: Reverse gyrase, TOPRIM domain (domain) [506-672]
- IPR013497: DNA topoisomerase, type IA, central (domain) [626-1054]
- IPR023405: DNA topoisomerase, type IA, core domain (homologous_superfamily) [502-1052]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [59-228]
- IPR005736: Reverse gyrase (family) [1-1054]
- IPR003601: DNA topoisomerase, type IA, domain 2 (domain) [652-738]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [3-504]
- IPR006171: TOPRIM domain (domain) [506-662]
Sequence:
MIPVVYSNLCPVCGGDLESKEIEKHVCFRKKRSLCLFPEDFLLKEFVEFFRKCVGEPRAIQKMWAKRILRKESFAATAPTGVGKTSFGLAMSLFLALKGKRCYVIFPTSLLVIQAAETIRKYAEKAGVGTENLIGYYHGRIPKREKENFMQNLRNFKIVITTTQFLSKHYRELGHFDFIFVDDVDAILKASKNVDKLLHLLGFHYDLKTKSWVGEARGCLMVSTATAKKGKKAELFRQLLNFDIGSSRITVRNVEDVAVNDESISTLSSILEKLGTGGIIYARTGEEAEEIYESLKNKFRIGIVTATKKGDYEKFVEGEIDHLIGTAHYYGTLVRGLDLPERIRFAVFVGCPSFRVTIEDIDSLSPQMVKLLAYLYRNVDEIERLLPAVERHIDEVREILKKVMGKERPQAKDVVVREGEVIFPDLRTYIQGSGRTSRLFAGGLTKGASFLLEDDSELLSAFIERAKLYDIEFKSIDEVDFEKLSRELDESRDRYRRRQEFDLIKPALFIVESPTKARQISRFFGKPSVKVLDGAVVYEIPMQKYVLMVTASIGHVVDLITNRGFHGVLVNGRFVPVYASIKRCRDCGYQFTEDRESCPKCGSENVDNSRSRIEALRKLAHDAEFVIVGTDPDTEGEKIAWDLKNLLSGCGAVKRAEFHEVTRRAILEALESLRDVDENLVKAQVVRRIEDRWIGFVLSQKLWERFNNRNLSAGRAQTPVLGWIIDRFQESRERRKIAIVRDFDLVLEHDEEEFDLTIKLVEEREELRTPLPPYTTETMLSDANRILKFSVKQTMQIAQELFENGLITYHRTDSTRVSDVGQRIAKEYLGDDFVGREWGESGAHECIRPTRPLTRDDVQRLIQEGVLVVEGLRWEHFALYDLIFRRFMASQCRPFKVVVKKYSIEFDGKTAEEERIVRAEGRAYELYRAVWVKNELPTGTFRVKAEVKSVPKVLPFTQSEIIQMMKERGIGRPSTYATIVDRLFMRNYVVEKYGRMIPTKLGIDVFRFLVRRYAKFVSEDRTRDLESRMDAIERGELDYLKALEDLYAEIKSID
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0004386 (helicase activity), GO:0008094 (ATP-dependent activity, acting on DNA), GO:0005515 (protein binding), GO:0140097 (catalytic activity, acting on DNA), GO:0003678 (DNA helicase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0090304 (nucleic acid metabolic process), GO:0006265 (DNA topological change), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex)