Protein: 3alpha-hydroxy bile acid-CoA-ester 3-dehydrogenase 2 (P19337)
Organism: Clostridium scindens (strain JCM 10418 / VPI 12708)
Function: Involved in the multi-step bile acid 7alpha-dehydroxylation pathway that transforms primary bile acids to secondary bile acids in the human gut. Catalyzes the oxidation of C3-hydroxyl group of CoA conjugated bile acids generating a C3-oxo bile acid intermediate. Can use choloyl-CoA, chenodeoxycholoyl-CoA, deoxycholoyl-CoA, and lithocholoyl-CoA as substrates with similar efficiency. Highly prefers NAD over NADP as cosubstrate. Also catalyzes the reverse reactions; in vitro, the preferred direction of reaction depends on the pH. Has very little activity with unconjugated (non-CoA) bile acid substrates
InterPro domains:
- IPR002347: Short-chain dehydrogenase/reductase SDR (family) [7-231]
- IPR020904: Short-chain dehydrogenase/reductase, conserved site (conserved_site) [144-172]
- IPR036291: NAD(P)-binding domain superfamily (homologous_superfamily) [5-246]
- IPR050259: Short-chain dehydrogenases/reductases (family) [2-248]
Sequence:
MNLVQDKVTIITGGTRGIGFAAAKIFIDNGAKVSIFGETQEEVDTALAQLKELYPEEEVLGFAPDLTSRDAVMAAVGQVAQKYGRLDVMINNAGITSNNVFSRVSEEEFKHIMDINVTGVFNGAWCAYQCMKDAKKGVIINTASVTGIFGSLSGVGYPASKASVIGLTHGLGREIIRKNIRVVGVAPGVVNTDMTNGNPPEIMEGYLKALPMKRMLEPEEIANVYLFLASDLASGITATTVSVDGAYRP
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0016614 (oxidoreductase activity, acting on CH-OH group of donors), GO:0016229 (steroid dehydrogenase activity), GO:1901265 (nucleoside phosphate binding), GO:0043177 (organic acid binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0016616 (oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0070403 (NAD+ binding), GO:0031406 (carboxylic acid binding), GO:0017076 (purine nucleotide binding), GO:0033764 (steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor), GO:0033293 (monocarboxylic acid binding), GO:0030554 (adenyl nucleotide binding), GO:0051287 (NAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:0016043 (cellular component organization), GO:1901700 (response to oxygen-containing compound), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006629 (lipid metabolic process), GO:0010033 (response to organic substance), GO:0006082 (organic acid metabolic process), GO:0022607 (cellular component assembly), GO:0014070 (response to organic cyclic compound), GO:0043933 (protein-containing complex organization), GO:0043436 (oxoacid metabolic process), GO:0033993 (response to lipid), GO:0008206 (bile acid metabolic process), GO:0008202 (steroid metabolic process), GO:0065003 (protein-containing complex assembly), GO:0019752 (carboxylic acid metabolic process), GO:0051259 (protein complex oligomerization), GO:0032787 (monocarboxylic acid metabolic process), GO:0051260 (protein homooligomerization), GO:0051262 (protein tetramerization), GO:0051289 (protein homotetramerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)