Protein: Reverse gyrase subunit B (F1SVH1)
Organism: Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Function: Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process; dATP also allows positive supercoiling. Increases the linking number in steps of +1. Only this subunit binds ATP, it does so in a DNA- and RgyA-independent manner. Hydrolyzes ATP only in the presence of DNA. The RgyA subunit transiently cleaves a single DNA strand and remains covalently bound to the 5' DNA end probably through a tyrosine residue. It changes linking number in steps of one, and nicks DNA preferentially at 5'-CNNN | 3'-sites with a strong preference for 4 pyrimidine residues. There are about 1000 heterodimers per cell. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication (By similarity)
Subcellular location: Cytoplasm
InterPro domains:
- IPR040569: Reverse gyrase, zinc finger (domain) [56-97]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [153-414]
- IPR001650: Helicase, C-terminal domain-like (domain) [424-600]
- IPR013824: DNA topoisomerase, type IA, central region, subdomain 1 (homologous_superfamily) [957-1117]
- IPR034142: Reverse gyrase, TOPRIM domain (domain) [779-947]
- IPR013497: DNA topoisomerase, type IA, central (domain) [899-1221]
- IPR023405: DNA topoisomerase, type IA, core domain (homologous_superfamily) [778-1140]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [161-302]
- IPR005736: Reverse gyrase (family) [60-1161]
- IPR003601: DNA topoisomerase, type IA, domain 2 (domain) [926-1123]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [138-777]
- IPR006171: TOPRIM domain (domain) [779-935]
Sequence:
MVLKRAADMVPKGFRDLVEPILDDCADLEELADRVVETEMEPDEVRRRDVGNTDSNEPVAIFGSSCVLCGGDCSSVRLTSRIGICERCLPVDTETLREVLKEARKRHGYVGEALLMFILVERYSPDRVEEFFRRYVWPELFTEIVDRVFDRATGFRLYSAQRVWTRRLVKGCSFSILAPTGTGKTSWGSLVAAVFGHAGRRVYYLVPTTTLVRQVENRIKGFARDAELDVDVVAYHAAMPTQAKREALERISSGDFDVLITTAQFLVHRVEDLEKLNFDLILVDDVDAIIRGTGRNVDRVLRVAGLEQEEIDSAYRLATLRRRYYSLRDWLRSLEDRGDKRAERVREELREVEREIEELEELLKRVKKERDLARIVFMSATGAAAPSRRLAVVRELFDFEVGAGGEGLRNIQDIAVISEPSPEAVERIVRKAGVKGGLIFVPQRLPGEKKAREIVEELAEHLRSSGIEARAIHAGTPAEEREEAIDGFSEGDVDVLVAVASPYGVIVRGLDLPQAARYAVFYGVPRQRIRLTPREEDLKDPTYVASALSNLARLLDDRRARSRLEGVAGRLWRIIRRGTWIRERLEEAVEPLSLNTLMKLAKRDPEDIAEQLDVDRWLARHVQTLAEGVRELTRLLGDPDRVKALAEEATTVAVYEEGEEAYLEVPDLRTYIQASGRVSRLFAGGVTFGLSFVLCPEDERELRTLNGLIRRMSYTYGSEFEWRSYPKSLDMKEIGLELKEISDEELEELVRKVDEDRERVRKVLAGELKPEETGRLARSALMIVESPNKARMIASLFSQRPSRRRLNGGVAYEAAADGLHLTVVATQGHVADLVEEPGVHGVLRIDERWVPMYDVLGRCSECGEQVVGSEECPNCGGEVELKTPLLESIRELASEADVILIGTDPDTEGEKIGWDVFNYLGWTTAQVYRTEFHEVTRRGISEALKEESWKNVDAGRVSAQILRRVADRWIGFSLSQDLWDVFKHLEIKLGELPSGSRIEVRLDIPSGVEVVDFRRTFDEDSSVRSRSVRLRREGDEYVVRTRISRGGDVTYTATLLDPNRKLGDRNGVRPELVRVRASVNGEPVDPNVKLEPMTWLSAGRVQTPVLGWIIDRAREYRETEFYACRAEVPADDVTIRALIEELKVPRALTEKLDEATIRVLSKIAEEGPDAEFSEEEVGRFTETELFERKDGRYRLSEEGRKVLESEGVIGLMLHLAGVSGR
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0090304 (nucleic acid metabolic process), GO:0006265 (DNA topological change), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0000428 (DNA-directed RNA polymerase complex), GO:0061695 (transferase complex, transferring phosphorus-containing groups)