Protein: Histone-lysine N-methyltransferase set-25 (G5EEU2)
Organism: Caenorhabditis elegans
Function: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using mono- and dimethylated H3 'Lys-9' as substrate. Acts redundantly with the methyltransferase met-2 to position chromosome arms at the nuclear lamina. Required for small-RNA-induced H3K9 methylation. Together with met-2, protects and stabilizes repeat-rich genomic regions by suppressing transcription- induced replication stress through methylation of H3K9 . Suppresses transcription from various repetitive genomic elements through methylation of H3K9; exposure to moderately raised temperature reduces methylation by set-25 leading to de- repression of transcription of repetitive elements lasting at least 3 subsequent generations, despite culture at lower temperatures . May play a role in transgenerational transmission of environmental information
Subcellular location: Nucleus. Chromosome . Nucleus lamina . Note=Colocalizes with its own product and hpl-1 in foci in the peripheral region of the nucleus, in a manner dependent on H3K9me3
InterPro domains:
- IPR001214: SET domain (domain) [547-696]
- IPR050973: Histone-lysine N-methyltransferase, H3 Lys-9 specific (family) [145-712]
- IPR046341: SET domain superfamily (homologous_superfamily) [414-711]
Sequence:
MLPAWGTSTEATASHAGWDGDDEGDIRAAYTEDEKKENIPPISLTSVSTNGAYPGQKRRRSESVRTLKPECPPEETQRLRQRRRISATDATQSSRTMNVIEDRKPRVNRARKSQDAPSTSTCGFETPVGTKRKSKAADSQKPPKQSKLRKIDEASTSKAVDNSSKDGKKTTKPAVTQSNRRRSGLSLRPVPIETIFSESSGRESSTEDEADVSHQQRVEKIAKNPIMVVVLPLGPGNYPNNERITVVSTYKSRVNKNCNEARRAQRHGSWSRKGIAFPGIPTKKFTKSDLAKYGAHASNWPAQAAFRSEEGKILIYYEGWTCLTLHRLSVEECARTAPTILEEMSIRDKFIETVKSAAAEEAKLVVEKNQENGIELTLDEALKQIFIEPVPQSSPENVFWIYQDLSYFHTMDNRDLGLAPVFYISSYTQSVRPPCYAYTAINIVDVDAYKRCLESRANMSFADLTGQKIWMPTRSKACENGTKCKCDARFMFLYDPHDVTNLECTPDGKVDFTDFKIDNARIVMECSDACGCSLDCPRRSLQRGQQHPLAVYYEGPEKGFGVRAAANIKAGELVCEYTGDVTLLPTSDPVASSSTKTDDGEEQENPEAPERVDSSYDAAFNAMDTKIIISAKKTGNISRFINHSCDPSSVFVEVYSRRFEEDPLIPRVAVYAIKDIALGEEITIAYYEPGIEWKRSSVKCRCKSTKCMGTLPAF
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008276 (protein methyltransferase activity), GO:0016279 (protein-lysine N-methyltransferase activity), GO:0042054 (histone methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0018024 (histone lysine N-methyltransferase activity), GO:0140938 (histone H3 methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0008170 (N-methyltransferase activity), GO:0016278 (lysine N-methyltransferase activity), GO:0046974 (histone H3K9 methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0009605 (response to external stimulus), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0009892 (negative regulation of metabolic process), GO:0009991 (response to extracellular stimulus), GO:1901564 (organonitrogen compound metabolic process), GO:0042594 (response to starvation), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0043414 (macromolecule methylation), GO:0031323 (regulation of cellular metabolic process), GO:0080090 (regulation of primary metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006479 (protein methylation), GO:0031667 (response to nutrient levels), GO:0051246 (regulation of protein metabolic process), GO:0031667 (response to nutrient levels), GO:0051248 (negative regulation of protein metabolic process), GO:0051247 (positive regulation of protein metabolic process), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0008213 (protein alkylation), GO:0018022 (peptidyl-lysine methylation), GO:0031399 (regulation of protein modification process), GO:0031400 (negative regulation of protein modification process), GO:0016571 (histone methylation), GO:0018193 (peptidyl-amino acid modification), GO:0016570 (histone modification), GO:0034968 (histone lysine methylation), GO:0061647 (histone H3-K9 modification), GO:0031056 (regulation of histone modification), GO:0018205 (peptidyl-lysine modification), GO:1901983 (regulation of protein acetylation), GO:0019941 (modification-dependent protein catabolic process), GO:0035067 (negative regulation of histone acetylation), GO:2000757 (negative regulation of peptidyl-lysine acetylation), GO:2000756 (regulation of peptidyl-lysine acetylation), GO:0035065 (regulation of histone acetylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0043229 (intracellular organelle), GO:0000792 (heterochromatin), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome)