Protein: NAD-dependent protein deacylase SIR5 (A0A0J9UVG7)
Organism: Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935
Function: NAD-dependent protein-lysine deacylase that decrotonylates the PDC (pyruvate dehydrogenase complex) subunit LAT1 at 'Lys-148' to inhibit PDC activity and consequently ATP production. Also decrotonylates histone H3 crotonylated at 'Lys-18' (H3K18cr), to repress the expression of genes involved in aerobic respiration . May also act as a NAD-dependent deacetylase (By similarity). Does not mediate desuccinylation, demalonylation, or deglutarylation of LAT1
Subcellular location: Mitochondrion. Cytoplasm, cytosol. Nucleus . Chromosome
InterPro domains:
- IPR003000: Sirtuin family (family) [53-261]
- IPR027546: Sirtuin, class III (family) [46-311]
- IPR026591: Sirtuin, catalytic core small domain superfamily (homologous_superfamily) [63-229]
- IPR050134: NAD-dependent sirtuin protein deacylases (family) [32-313]
- IPR026590: Sirtuin family, catalytic core domain (domain) [28-324]
- IPR029035: DHS-like NAD/FAD-binding domain superfamily (homologous_superfamily) [19-314]
Sequence:
MRLLRPTPRLSSIFSSKTATSNLRFFTAMAPHNDVGAFHEALRSSKRILALCGAGLSASSGLPTFRGAGGLWRNHDATSLATLSAFKNDPGLVWLFYNYRRHMCLRAEPNPAHYALAALAEKNKDFLCLTQNVDNLSQQAGHPQDQLRTLHGSLFDIKCTNCDWIQRGNYDDPFCPALAPASVDVEPGKPFPLLDASLPLDPISPDDIPKCPQCKIGFQRPGVVWFGENLDEVMMMGITNWLLEDKVDLMLVIGTSAQVYPAAGYIDKAKRKGARIAVINPEAENEEEMYKVKPGDFAFGKDAAEYLPLLLEPVIGKLETDKKERS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0140096 (catalytic activity, acting on a protein), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0043169 (cation binding), GO:0033558 (protein lysine deacetylase activity), GO:0046872 (metal ion binding), GO:0034979 (NAD-dependent protein deacetylase activity), GO:0016811 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides), GO:0070403 (NAD+ binding), GO:0017076 (purine nucleotide binding), GO:0046914 (transition metal ion binding), GO:0030554 (adenyl nucleotide binding), GO:0008270 (zinc ion binding), GO:0051287 (NAD binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0009605 (response to external stimulus), GO:0019222 (regulation of metabolic process), GO:0009607 (response to biotic stimulus), GO:0051707 (response to other organism), GO:0009892 (negative regulation of metabolic process), GO:0043207 (response to external biotic stimulus), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009617 (response to bacterium), GO:0060255 (regulation of macromolecule metabolic process), GO:0009615 (response to virus), GO:2000377 (regulation of reactive oxygen species metabolic process), GO:0042742 (defense response to bacterium), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)