Protein: Internalin A (P0DJM0)
Organism: Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Function: Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells. Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin- 2 function as receptors. Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA
Subcellular location: Secreted, cell wall; Peptidoglycan-anchor. Secreted . Note=In the absence of SrtA in exponential phase some protein is still anchored to the cell wall and a very small amount is secreted while overall protein levels are the same in the srtA mutant; in stationary phase almost no protein accumulates. During exponential growth detected on the cell surface as a series of dots in a helical pattern, it is excluded from the septum; if expression is increased it accumulates at cell poles. The helical pattern of InlA does not overlap with that of InlH, InlJ or Hbp2 (SvpA) . In stationary phase colocalizes with InlH at cell poles and at the septum, the location shift requires SigB
InterPro domains:
- IPR014755: Copper resistance protein CopC/internalin, immunoglobulin-like (homologous_superfamily) [415-496]
- IPR013378: Internalin B-like, B-repeat (repeat) [498-706]
- IPR003591: Leucine-rich repeat, typical subtype (repeat) [97-405]
- IPR032675: Leucine-rich repeat domain superfamily (homologous_superfamily) [32-414]
- IPR025875: Leucine rich repeat 4 (repeat) [99-381]
- IPR024634: Internalin, N-terminal (domain) [33-76]
- IPR050836: SDS22/Internalin LRR-containing (family) [23-222]
- IPR001611: Leucine-rich repeat (repeat) [99-405]
- IPR014756: Immunoglobulin E-set (homologous_superfamily) [417-495]
- IPR042229: Listeria-Bacteroides repeat domain superfamily (homologous_superfamily) [497-706]
- IPR012569: Internalin, Ig-like inter-repeat region (domain) [439-495]
- IPR019931: LPXTG cell wall anchor domain (domain) [761-800]
- IPR047600: Internalin A (family) [2-800]
Sequence:
MRKKRYVWLKSILVAILVFGSGVWINTSNGTNAQAATITQDTPINQIFTDTALAEKMKTVLGKTNVTDTVSQTDLDQVTTLQADRLGIKSIDGVEYLNNLTQINFSNNQLTDITPLKNLTKLVDILMNNNQIADITPLANLTNLTGLTLFNNQITDIDPLKNLTNLNRLELSSNTISDISALSGLTSLQQLSFGNQVTDLKPLANLTTLERLDISSNKVSDISVLAKLTNLESLIATNNQISDITPLGILTNLDELSLNGNQLKDIGTLASLTNLTDLDLANNQISNLAPLSGLTKLTELKLGANQISNISPLAGLTALTNLELNENQLEDISPISNLKNLTYLTLYFNNISDISPVSSLTKLQRLFFYNNKVSDVSSLANLTNINWLSAGHNQISDLTPLANLTRITQLGLNDQAWTNAPVNYKANVSIPNTVKNVTGALIAPATISDGGSYTEPDITWNLPSYTNEVSYTFSQPVTIGKGTTTFSGTVTQPLKAIFNVKFHVDGKETTKEVEAGNLLTEPAKPVKEGHTFVGWFDAQTGGTKWNFSTDKMPTNDINLYAQFSINSYTATFDNDGVTTSQTVDYQGLLQEPTAPTKEGYTFKGWYDAKTGGDKWDFATSKMPAKNITLYAQYSANSYTATFDVDGKSTTQAVDYQGLLKEPKAPTKAGYTFKGWYDEKTDGKKWDFATDKMPANDITLYAQFTKNPVAPPTTGGNTPPTTNNGGNTTPPSANIPGSDTSNTSTGNSASTTSTMNAYDPYNSKEASLPTTGDSDNALYLLLGLLAVGTAMALTKKARASK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0042221 (response to chemical), GO:0070887 (cellular response to chemical stimulus), GO:0010035 (response to inorganic substance), GO:0010038 (response to metal ion), GO:0071241 (cellular response to inorganic substance), GO:0071248 (cellular response to metal ion), GO:0010039 (response to iron ion), GO:0071281 (cellular response to iron ion)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0009274 (peptidoglycan-based cell wall)