Protein: DNA-directed RNA polymerase subunit beta (Q4V967)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft
InterPro domains:
- IPR037034: RNA polymerase Rpb2, domain 2 superfamily (homologous_superfamily) [188-364]
- IPR015712: DNA-directed RNA polymerase, subunit 2 (family) [32-1130]
- IPR037033: DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily (homologous_superfamily) [669-1013]
- IPR007644: RNA polymerase, beta subunit, protrusion (domain) [36-407]
- IPR007642: RNA polymerase Rpb2, domain 2 (domain) [186-367]
- IPR007645: RNA polymerase Rpb2, domain 3 (domain) [453-518]
- IPR007120: DNA-directed RNA polymerase, subunit 2, hybrid-binding domain (domain) [668-1027]
- IPR014724: RNA polymerase Rpb2, OB-fold (homologous_superfamily) [699-873]
- IPR009674: DNA-directed RNA polymerase I subunit RPA2, domain 4 (domain) [561-619]
- IPR007121: RNA polymerase, beta subunit, conserved site (conserved_site) [877-889]
- IPR007641: RNA polymerase Rpb2, domain 7 (domain) [1030-1130]
Sequence:
MDLSKWCNLKTEPSLKHLTEAGFGVPKEKQRVPVQELVKAHIESFDQAVTDGLCRVVEAIPPMEFLYKGDRISLSFVEAIIHTPSVSKGGISRDLRVFPAECRGRRCTYRAKLVADVSWSVNGVPKGIIKQSLGQMPIMVKSKLCNLHGLSPKELLEHHEEAEEMGGYFIVNGIEKVIRLLIMPRRNYPIAVSRPKFKSRGQGYTQYAISMRCVKEEHTAINMNLHYLDNGTVMVNFIYRKELFFLPLGFALKALVDYSDYQIYQELIKGREDNSFYKSCVSEMLRIVMDQGCTTKIKVLNYLGERFRVKLNLPEWFTHEQCAHFLLDECLCIHLKTDVEKFYMLCLMTRKLFSFAKQECMEENPDSPMFHEVLTPGQLYLMFLKEKMEGWLVSVKLALEKKPQRVGGLTPDSMTKLFNMGTDLTKAFEYLLATGNLMSKTGLGMLQNSGLCVVADKLNFIRYLSHFRCVHRGAAFAKMRTTTVRKLLPESWGFLCPVHTPDGEPCGLMNHMTAHCEIVAPVYPTSTLPGLLCSLGVNAADGNPGQAYSDCYPVILDGAMIGWVEKEVAPSVIESLRRFKVLGEKKVPPWTEIVLVPQTGKASLYPGLYLFTTPCRMVRTVRNLSLGKQELIGIFEQVYLNVGIFEKEIEDGVTTHQELFPHSMLSVVAEFIPFSDHNQSPRNMYQCQMSKQTMGFPLHSYQYRSDNKLYRLQTPQSPLVRPAMYDHYDVDNYPIGTNAIVAVISYTGYDMEDAMIVNKSSWERGFAHGSVYKTEIIDLSEKVKGDDSIVFGVKPGDPKVMGKLDADGLPFIGSVLKYGDPFYGYISLNTGQSHVYFYKYQESCVVDNIKVCSNETGTGRFKRICVTSRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMSPDILFNPHGFPSRMTIGMLIESMAGKSASLHGLCHDATPFTFSEENSALEHFGELLKAAGYNYYGTERLYSGLSGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPMGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAQVCMDCGSLLSTLLEKPPPNWSATRHRKTICLLCNKSDSIETVSVPYVFKYFVAELAAMNIKIKLDVK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0097747 (RNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0034062 (5'-3' RNA polymerase activity), GO:0003899 (DNA-directed 5'-3' RNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0048870 (cell motility), GO:0009653 (anatomical structure morphogenesis), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0009888 (tissue development), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048513 (animal organ development), GO:0009790 (embryo development), GO:0014029 (neural crest formation), GO:0016477 (cell migration), GO:0048863 (stem cell differentiation), GO:0048864 (stem cell development), GO:0009792 (embryo development ending in birth or egg hatching), GO:0060485 (mesenchyme development), GO:0048762 (mesenchymal cell differentiation), GO:0001667 (ameboidal-type cell migration), GO:0014033 (neural crest cell differentiation), GO:0043009 (chordate embryonic development), GO:0001837 (epithelial to mesenchymal transition), GO:0014032 (neural crest cell development), GO:0090497 (mesenchymal cell migration), GO:0001755 (neural crest cell migration)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140535 (intracellular protein-containing complex), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:0043227 (membrane-bounded organelle), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0070013 (intracellular organelle lumen), GO:0005736 (RNA polymerase I complex), GO:0005730 (nucleolus), GO:0005634 (nucleus), GO:0031981 (nuclear lumen), GO:0005694 (chromosome)