Protein: General transcription and DNA repair factor IIH subunit TFB5 (Q9N390)
Organism: Caenorhabditis elegans
Function: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and in RNA transcription by RNA polymerase II (By similarity). In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment (By similarity). In transcription, TFIIH has an essential role in transcription initiation (By similarity). When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape (By similarity). Necessary for the stability of the TFIIH complex and for the presence of normal levels of TFIIH in the cell. Required for efficient binding of TFIIH to damaged DNA. Dispensable for normal development, but required when transcription is challenged
Subcellular location: Nucleus. Chromosome. Note=Localized to paired homologous chromosomes (bivalents) about 10 minutes after UV irradiation, but localization is lost after 35 minutes, probably as a result of successful DNA damage repair as part of the TFIIH complex
InterPro domains:
- IPR035935: TFB5-like superfamily (homologous_superfamily) [1-71]
- IPR009400: TFIIH subunit TTDA/Tfb5 (family) [1-68]
Sequence:
MVNVKKGVLVTSDPAFRQLLIHLDDSRQLGSKFIVRELDDTHLFIEKEIVPMLENKVEQIMENMNPEAVDK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0009628 (response to abiotic stimulus), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009314 (response to radiation), GO:0033554 (cellular response to stress), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0009416 (response to light stimulus), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0006259 (DNA metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0010467 (gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0009411 (response to UV), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006281 (DNA repair), GO:0006289 (nucleotide-excision repair), GO:0006351 (DNA-templated transcription), GO:0006366 (transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)