Protein: Reverse gyrase 1 (Q08582)
Organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
Function: Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. Increases the linking number in steps of +1. Has a DNA-stimulated ATPase activity; closed circular ssDNA stimulates ATPase much better than dsDNA although negative supercoiled, positive supercoiled and relaxed dsDNA all stimulate ATPase activity . All NTPs permit topoisomerization (relaxation) of negatively supercoiled dsDNA without nucleotide hydrolysis . It transiently cleaves a single DNA strand and remains covalently bound to the 5' DNA end. Acts via a tyrosine residue. Reverse gyrase binds and unwinds DNA independently of ATP binding and DNA cleavage. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication, probably acts via ssDNA regions of the chromosome (Probable)
Subcellular location: Cytoplasm
InterPro domains:
- IPR003593: AAA+ ATPase domain (domain) [101-326]
- IPR040569: Reverse gyrase, zinc finger (domain) [7-44]
- IPR013826: DNA topoisomerase, type IA, central region, subdomain 3 (homologous_superfamily) [928-1055]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [84-330]
- IPR003602: DNA topoisomerase, type IA, DNA-binding domain (domain) [918-1200]
- IPR013824: DNA topoisomerase, type IA, central region, subdomain 1 (homologous_superfamily) [810-1243]
- IPR034142: Reverse gyrase, TOPRIM domain (domain) [625-801]
- IPR013497: DNA topoisomerase, type IA, central (domain) [753-1248]
- IPR023405: DNA topoisomerase, type IA, core domain (homologous_superfamily) [623-1246]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [92-293]
- IPR005736: Reverse gyrase (family) [7-1248]
- IPR003601: DNA topoisomerase, type IA, domain 2 (domain) [780-886]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [33-615]
- IPR006171: TOPRIM domain (domain) [625-789]
Sequence:
MQSLSDIPPSIYLFSCPNCGRSISTYRLLLGSVCNICLEEDKEYKNIGDLIKDIEKQGNLIKLKDIQRVLDDYESFVSVFRRLLGFPPFGPQKSWIYRLLSGESFAIIAPPGLGKTTFGLISSIYLYLRGKKSILVFPTKSLVRQAIDKLSSYIQNLAEIKENPPKVIYYYSGMSASERKEADEGLQSKTFDIFISTNRFLIDNIDQISSTSYQFLFVDDVDTALKSSKSAQAILKLLGFTPSDQDKIKESLKKYRENTQKNEQNEYIFEEIDKIRKDRLASKTVIFSSATLNRSNPILTSLVGFKPGSSVIYIRKVYDMYVKQPDKEQETFNLIKSLLHRLGDGGLIFVPVDKKQEYIKRLQSELSNEFNVAAITSTSATKIDDFANGEIDVLIGSATHYGILVRGLDLPWRVKYSIFVGIPKFKFRLGEKVNLLTLSRLLSLIALITKDQEVIYISRRVKDKIRRLSPAALTMLSVQAKEGKLEDSILLKAYDLLNKYLSNQNVLKRISEIGDFVLSPDNDILIPDYLTYVQASGRTSRIYAGDVTTGLSILLVDDFNLFRLLNKKLQYILDDIQWRELDVEKWTAGDVEIKNLISKINEERNEISKLKNEGNVAPALQKVKTVLLVVESPNKAKTISSFFSRPSIRQIGNMRVYETVLGDKVLMVTASGGHVYDLTTQDMGIYGVDIMKQNSSLVFIPIYNSIKKCENNHQFTDFFESNKCPRCMTTKVRYDSLKSINVLRNLAVEADEVLIGTDPDTEGEKIAWDLYLALRPYNSNIRRAEFHEVTRKAILQAINQPREFNVNLVKSQLVRRIEDRWIGFKLSSILQTRFWPEYCKSLSSNKQLNCNENKNLSAGRVQTPVLSWIVDRYTEYQRNKSRVYYGKIDQLQDIVIYVPKQDGVRKNSKIVVVFNEINQLEEEFGPLPPYTTDTLLSDSNNFFGLSAPETMRIAQDLFELGLITYHRTDSNRISNTGISVAENYLKDVLGDKYTNIFKPRSWGDGGAHEGIRPTKPIDVEQLRLLIEEGELELAKRLTNNHFKVYDIIFRRFISSQIIPLKVRKEIVKIELYGENKKEKINSNQNIIEVITGITLPGIDTEISKFAYVPVRNVSRSVAERLKELGRSIPTDFSIEISNSFIKSTVNLYTQADLVMEMKNKKIGRPSTYATIIGTILRRGYVLESLKTKKLIPTRLGVEVNKYLNENYGRFVSEDRTRKLLQLMDMVEAGQEKYEEVLKQVYEEINEIR
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016740 (transferase activity), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0003677 (DNA binding), GO:0097747 (RNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0003697 (single-stranded DNA binding), GO:0034062 (5'-3' RNA polymerase activity), GO:0003899 (DNA-directed 5'-3' RNA polymerase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0090304 (nucleic acid metabolic process), GO:0006265 (DNA topological change), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0000428 (DNA-directed RNA polymerase complex), GO:0061695 (transferase complex, transferring phosphorus-containing groups)