Protein: Perforin-like protein 1 (V5BCL0)
Organism: Toxoplasma gondii (strain ATCC 50861 / VEG)
Function: Pore-forming protein that promotes parasite exit from host cells: mediates formation of a pore in the parasitophorous vacuolar membrane, leading to membrane permeabilization, thereby facilitating parasite egress from host cells. May also form a pore in the host plasma membrane . Preferentially binds inner leaflet lipids, such as phosphatidylethanolamine (PE) or phosphatidylserine (PS)
Subcellular location: Parasitophorous vacuole membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, microneme membrane; Multi-pass membrane protein
InterPro domains:
- IPR020864: Membrane attack complex component/perforin (MACPF) domain (domain) [463-817]
Sequence:
MFVVDLSLSGNMRSLTHGKSIHRSSIVVVTHCSHRLSLSPLNRPALFPSHSDRYTVPWRCRPSSWRLWITFVCLLTLHMFGLSSAVKRDGEVPTANLRASLSPDSSKEDAMLLSAIGGIGPTESRLQHIPFRTTRYLPIRSHVLQSTVERIESPEALNEVPTKVERNEFTEKGDKTEQVLTAQADHKSLLEGRSESTSTAPDDDFDFLFEDDTPKKPKSRVNKGTSSDETSPGDRSSGEGSSASDSLLSVVHRTKGNATQANKNQKRITHPKSKAQHQKKVTKKQGIPGSDSLGTSSADFDFDIDSSSNTQADDSQRQSLGDSSALDLFGSDTGDTGDIFGFSNSSSDGGNAAANDGGLFSSSGMGPTGASDETSANPLGSILGGVLNPFAQQQSITPMTDKADEWSKENMKAFEEKMPKKMRDDGSPDSWYKQAVPQESVADLNRMRENRRIAEENRAAAPLSAVYTKATKTVPAINYLGAGYDHVRGNPVGDPSSMGDPGIRPPVLRFTYAQNEDGVSNDLTVLQPLGGYVRQYVACRQSETISELSNLSDYQNELSVDASLQGGDPIGLNSFSASTGYRDFAKEVSKKDTRTYMLKNYCMRYEAGVAQSNHLKWNVTLAFAAGVSQLPDVFDAHNPECACSAEQWRQDQNAEACTKTNVPIWISFIEQFGTHFLVRLFAGGKMTYQVTAKRSEVEKMRNMGIDVKTQLKMQLGGVSGGAGQGTSSKKNQSSSEYQMNVQKETLVIGGRPPGNVSDPAALAAWADTVEELPMPVKFEVQPLYHLLPVEKQEAFKQAVTFYSKAVGLTPQDLSALTGVTRNLPKQLTQATQVAWSGPPPGFAKCPAGQVVILGFAMHLNFKEPGTDNFRIISCPPGREKCDGVGTASSETDEGRIYILCGEEPINEIQQVVAESPAHAGASVLEASCPDETVVVGGFGISVRGGSDGLDSFSIESCTTGQTICTKAPTRGSEKNFLWMMCVDKQYPGLRELVNVAELGSHGNANKRAVNSDGNVDVKCPANSSIVLGYVMEAHTNMQFVRDKFLQCPENASECKMTGKGVDHGMLWLFDRHALFGWIICKTVNEPAMHVATDVGKAKGNGKKKKGKKGKNKTNAPNEVEEGQQLGADSPSQVSVPADADSGPTSKTMSSLKLAPVKLLDL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005215 (transporter activity), GO:0022857 (transmembrane transporter activity), GO:0022803 (passive transmembrane transporter activity), GO:0015267 (channel activity), GO:0022829 (wide pore channel activity)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0043933 (protein-containing complex organization), GO:0022607 (cellular component assembly), GO:0065003 (protein-containing complex assembly), GO:0051259 (protein complex oligomerization), GO:0051260 (protein homooligomerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0005773 (vacuole), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0030139 (endocytic vesicle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0005764 (lysosome), GO:0045335 (phagocytic vesicle), GO:0097708 (intracellular vesicle), GO:0032010 (phagolysosome), GO:0005767 (secondary lysosome), GO:0020020 (food vacuole)