lokahq/bioreason-rl huggingface dataset:
Protein: D-amino-acid oxidase (P80324)
Organism: Rhodotorula toruloides (Yeast) (Rhodosporidium toruloides)
Function: Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients (By similarity)
Subcellular location: Peroxisome matrix
InterPro domains:
- IPR023209: D-amino-acid oxidase (family) [3-358]
- IPR006076: FAD dependent oxidoreductase (domain) [6-351]
- IPR006181: D-amino acid oxidase, conserved site (conserved_site) [327-345]
Sequence:
MHSQKRVVVLGSGVIGLSSALILARKGYSVHILARDLPEDVSSQTFASPWAGANWTPFMTLTDGPRQAKWEESTFKKWVELVPTGHAMWLKGTRRFAQNEDGLLGHWYKDITPNYRPLPSSECPPGAIGVTYDTLSVHAPKYCQYLARELQKLGATFERRTVTSLEQAFDGADLVVNATGLGAKSIAGIDDQAAEPIRGQTVLVKSPCKRCTMDSSDPASPAYIIPRPGGEVICGGTYGVGDWDLSVNPETVQRILKHCLRLDPTISSDGTIEGIEVLRHNVGLRPARRGGPRVEAERIVLPLDRTKSPLSLGRGSARAAKEKEVTLVHAYGFSSAGYQQSWGAAEDVAQLVDEAFQRYHGAARESKL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0036094 (small molecule binding), GO:1901363 (heterocyclic compound binding), GO:0043167 (ion binding), GO:0097159 (organic cyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0016638 (oxidoreductase activity, acting on the CH-NH2 group of donors), GO:1901265 (nucleoside phosphate binding), GO:0043168 (anion binding), GO:0000166 (nucleotide binding), GO:0050660 (flavin adenine dinucleotide binding), GO:0016641 (oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor), GO:0071949 (FAD binding), GO:0008131 (primary amine oxidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:1901575 (organic substance catabolic process), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0016054 (organic acid catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0009063 (amino acid catabolic process), GO:0009068 (aspartate family amino acid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901606 (alpha-amino acid catabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0009066 (aspartate family amino acid metabolic process), GO:0006553 (lysine metabolic process), GO:0006554 (lysine catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)
Protein: D-aspartate oxidase (C4R6B0)
Organism: Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Function: Selectively catalyzes the oxidative deamination of acidic amino acids. Protects the organism from the toxicity of D-amino acids (By similarity). Enables the organism to utilize D- amino acids as a source of nutrients. Enables the organism to utilize D-aspartate as a nitrogen source
InterPro domains:
- IPR023209: D-amino-acid oxidase (family) [1-371]
- IPR006076: FAD dependent oxidoreductase (domain) [4-368]
Sequence:
MATVCVVGSGILGLAVASCLLEKTNVNIVIISDDFPHESSHDLKYSPFFTSPWAGAHFRPFPSVTEFDQRHVEYTRATYGHFKKLAAFEPQEETSIRFLEGTDLVERGHPNFEYYSELKQGYREEIEDFVVNDWEHGFSASYKTWVLNAPFFLSYLFKKLRSNPRVTLKQGKLNTLREAFTENAAKNDQSPDLGANGYNYVFNCTGLGLQMNGGWDPACYVIRGQTLLLKVPSGPHLQKTVTHQSKEGQWTFFIPRPLSNPESPTEDYVILGGTKQEKDFDSGSPRSQDSLDILTRADRLFPELKDAKTGHFQVIQPNVGFRPSRKGGVRVEREKVPDVENSFAYHCYGAGGMGYELSFGVAFAVVDLFLDDYNQ
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016638 (oxidoreductase activity, acting on the CH-NH2 group of donors), GO:0016641 (oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor), GO:0008131 (primary amine oxidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0043094 (cellular metabolic compound salvage), GO:1901576 (organic substance biosynthetic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0008652 (amino acid biosynthetic process), GO:0016053 (organic acid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0043102 (amino acid salvage), GO:1901605 (alpha-amino acid metabolic process), GO:0019752 (carboxylic acid metabolic process), GO:0071267 (L-methionine salvage), GO:1901607 (alpha-amino acid biosynthetic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0046416 (D-amino acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005622 (intracellular anatomical structure)
Protein: D-aspartate oxidase (A3KCL7)
Organism: Sus scrofa (Pig)
Function: Selectively catalyzes the oxidative deamination of acidic amino acids. Suppresses the level of D-aspartate in the brain, an amino acid that can act as an agonist for glutamate receptors (By similarity). Protects the organism from the toxicity of D-amino acids (By similarity). May also function in the intestine (By similarity)
Subcellular location: Peroxisome matrix. Cytoplasm, cytosol . Note=Active in the peroxisomal matrix
InterPro domains:
- IPR023209: D-amino-acid oxidase (family) [3-336]
- IPR006076: FAD dependent oxidoreductase (domain) [5-324]
- IPR006181: D-amino acid oxidase, conserved site (conserved_site) [300-318]
Sequence:
MDTVRIAVVGAGVMGLSTAVCIFKLVPGCSITVISDKFTPETTSDVAAGMLIPPVYPDTPIHKQKQWFKDTFDHLFAIANSAEAKDAGVLLVSGWQIFQSAPTEEVPFWADVVLGFRKMTKNELKKFPQHVCGQAFTTLKYEGPTYLPWLEKRVKGSGGLVLTRRVEDLWELHPSFDIVVNCSGLGSKQLVGDMDIFPVRGQVLKVQAPWVKHFIRDGSGLTYIYPGLANVTLGGTRQKGDWNLSPNAEISKQILSRCCALEPSLRGACDIREKVGLRPSRPGVRLEKELLVQGSQRLPVVHNYGHGSGGIAMHWGTALEAARLVSECVQALRTPAPKSKL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016638 (oxidoreductase activity, acting on the CH-NH2 group of donors), GO:0016641 (oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor), GO:0008131 (primary amine oxidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:1901575 (organic substance catabolic process), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0016054 (organic acid catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0009063 (amino acid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901606 (alpha-amino acid catabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0046416 (D-amino acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)
Protein: D-aspartate oxidase (Q75WF1)
Organism: Vanrija humicola (Yeast) (Cryptococcus humicola)
Function: Selectively catalyzes the oxidative deamination of acidic amino acids. Protects the organism from the toxicity of D-amino acids . Enables the organism to utilize D-amino acids as a source of nutrients. Enables the organism to utilize D-aspartate as a source of nitrogen and carbon
Subcellular location: Peroxisome matrix
InterPro domains:
- IPR023209: D-amino-acid oxidase (family) [4-362]
- IPR006076: FAD dependent oxidoreductase (domain) [7-358]
Sequence:
MPPSDPIIVLGAGVIGLTTAVRLLEAHLGANVHILADHWPSDALDAQYASTIAGAHHLSFADDGDARQRRWDMRTFDVLYDEWKAVGERTGLMALTQTEMWEGATSHLAVYEGNPDFRVLDPRTAPCSNITHMVSFTSLTIAPTVYLAALEARVRDLGAKLHRAHVPSLGALRTDPALLALYTRPPAAVFVCAGLGARHLVPAPEAAALFPTRGQVVVVRAPWMRAGFTRQVGSLGGGEGGTRTYIIPRCNGEVVLGGTMEQGDWTPYPRDETVTDILTRALQICPDIAPPYARSWPKDDQVAALRSIVVRDAVGFRPSRAGGARVALASAAGMRVVYNYGHGGAGWQSCWGCAEDAVALWAGGAGGARL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016638 (oxidoreductase activity, acting on the CH-NH2 group of donors), GO:0016641 (oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor), GO:0008131 (primary amine oxidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0044237 (cellular metabolic process), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006520 (amino acid metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0044282 (small molecule catabolic process), GO:0033554 (cellular response to stress), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0016054 (organic acid catabolic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043436 (oxoacid metabolic process), GO:1901605 (alpha-amino acid metabolic process), GO:0009063 (amino acid catabolic process), GO:0019752 (carboxylic acid metabolic process), GO:1901606 (alpha-amino acid catabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0046416 (D-amino acid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)
Protein: HSPA8-interacting micropeptide miPEP155 (C0HMA1)
Organism: Homo sapiens (Human)
Function: Negatively regulates MHC class II antigen presentation in dendritic cells by interacting with the molecular chaperone HSPA8 and impairing its role in lysosomal antigen transport. Does not regulate the levels or activity of the MIR155HG microRNA from which miPEP155 is derived
Subcellular location: Cytoplasm. Nucleus
InterPro domains:
Sequence:
MEMALMVAQTRKGKSVV
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0005515 (protein binding), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0046983 (protein dimerization activity), GO:0042802 (identical protein binding), GO:0042803 (protein homodimerization activity), GO:0004518 (nuclease activity), GO:0004536 (deoxyribonuclease activity), GO:0004527 (exonuclease activity), GO:0004529 (DNA exonuclease activity), GO:0008408 (3'-5' exonuclease activity), GO:0016895 (DNA exonuclease activity, producing 5'-phosphomonoesters), GO:0016796 (exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0008296 (3'-5'-DNA exonuclease activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901575 (organic substance catabolic process), GO:0044248 (cellular catabolic process), GO:0009698 (phenylpropanoid metabolic process), GO:0046271 (phenylpropanoid catabolic process), GO:0009808 (lignin metabolic process), GO:0019439 (aromatic compound catabolic process), GO:1901361 (organic cyclic compound catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)
Protein: Cutinase (P0DX29)
Organism: Amycolatopsis mediterranei (strain S699) (Nocardia mediterranei)
Function: Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle (By similarity). Shows esterase activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) . Has a preference for medium chain length (C-4 to C-12) fatty acid esters. Active with p-nitrophenyl palmitate (p-NPP) as substrate. Hydrolyzes triacylglycerol substrates non-specifically with a preference for long, unsaturated fatty acyl chains with the highest activity for triolein. Substrates with cis-9 unsaturation are preferred over the saturated triacylglycerols . Hydrolyzes a wide range of natural oils, especially olive oil, with relatively high activity. Capable of catalyzing synthesis of the flavor ester isoamyl acetate by esterification of isoamyl alcohol using acetic acid as an acyl donor . Degrades synthetic aliphatic polyesters, namely poly(1,4-butylene succinate) extended with 1,6-diisocyanatohexane (PBSc-D) and poly(epsilon-caprolactone) (PCL) plastics . Does not degrade poly(lactic acid) (PLA) nor aromatic poly(ethylene terephthalate) (PET), the most abundant polyester plastic in the world
Subcellular location: Secreted
InterPro domains:
- IPR050261: FrsA esterase (family) [20-280]
- IPR029058: Alpha/Beta hydrolase fold (homologous_superfamily) [48-309]
- IPR041127: PET hydrolase/cutinase-like (domain) [47-306]
Sequence:
MSALTSQPTSSGSSEKIPRLRGWRAKAAGVVLAALALTTGVAAPAPAAANPYERGPDPTTASIEATSGSFATSTVTVSRLAVSGFGGGTIYYPTTTTAGTFGALSIAPGFTATQSSIAWLGPRLASQGFVVFTIDTLTTSDQPDSRGRQLLASLDYLTQQSSVRSRIDSTRLGVVGHSMGGGGTLEAARSRPTLQAAVPLTAWDLTKNWSTLQVPTLVVGAQSDTVAPVASHSIPFYTSLPSTLDRAYLELRGASHFAPNSPNTTIAKYTLSWLKRFIDNDTRYEQFLCPIPSTSLSISDYRGNCPHNG
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0052689 (carboxylic ester hydrolase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0044237 (cellular metabolic process), GO:0042221 (response to chemical), GO:0009056 (catabolic process), GO:0006805 (xenobiotic metabolic process), GO:0010033 (response to organic substance), GO:0009410 (response to xenobiotic stimulus), GO:0070887 (cellular response to chemical stimulus), GO:0044248 (cellular catabolic process), GO:0071466 (cellular response to xenobiotic stimulus), GO:0071310 (cellular response to organic substance), GO:0042178 (xenobiotic catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)
Protein: Polyglycine hydrolase (W6YKT9)
Organism: Cochliobolus carbonum (strain 26-R-13) (Maize leaf spot fungus) (Bipolaris
Function: Serine-type endopeptidase that cleaves Gly-Gly bonds in the polyglycine linker of host plant class IV chitinases to disrupt their chitin-binding, and thereby plays a role in lowering the defense responses of the host to the fungus (Ref.2, PubMed:21453431, PubMed:24627966, PubMed:25966977, PubMed:36762862). Degrades Z.mays Endochitinase A (CHIA) (Ref.2, PubMed:21453431, PubMed:24627966, PubMed:25966977, PubMed:36762862). Has low proteolytic activity on Z.mays Endochitinase B (CHIB)
Subcellular location: Secreted
InterPro domains:
- IPR050491: Beta-lactamase AmpC-like (family) [318-626]
- IPR012338: Beta-lactamase/transpeptidase-like (homologous_superfamily) [309-621]
- IPR049511: Polyglycine hydrolase-like, tandem repeat (repeat) [47-288]
- IPR001466: Beta-lactamase-related (domain) [317-572]
Sequence:
MYTSRSLFSTLASCLSLATLVASSPTTNQNLPSSKRDATVEQNVFFDINGKEVETRIAKLKAEGYRPTSLNIHGTPTDAKYAGIWIKQDGNPYETIVGANKTTYDAWLDQWKASGYVSTQVSATGPASNAVFAGVMEKIPSIRNWTQICGTSSPYAYLNATGDAPIVITGVSLYGTPTERQYCVLGHEDTDNHQQTVWFPTSSSQDYKTIEPAETTKRFWRPVFIDSSEDKLLASIFDDTSVGNWTVRTDLTASQLQSEITAQKANNMHPIHISGAGSTETQYVVIFTEQATPLERNWHAVGDITGFPDNARVKRDLDEILQKFMKRNSVRQAQVSASINGTVIASRAYTWAESNRAIVEPSDKFLLGSVSKAFTYAAVDHLVSTGVVNLTDHIYPLLGYTKPADPRSLNITIQHLLDHTGGFDRGMSPDIGFIFTTVAQSLNQSTPATLRQLIEYVLAKPLDFEPGTRSVYSNYGTLLLGYLIANKTGETYMSYLEKNVLKGLDVEMYTTPGADHTNDRIVQETKFTSISALTPLSEKRVANVYGGDGAVKEAAVSSFALKASADTVSRFIGKHAAYGLGPRQLFMYRDGTVAGARALAYSMAKLDWAITLNTREYLDENAWNTLVFTDLYQLWGALDSALPL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016811 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0051716 (cellular response to stimulus), GO:0042221 (response to chemical), GO:0070887 (cellular response to chemical stimulus), GO:0010035 (response to inorganic substance), GO:0010038 (response to metal ion), GO:0071241 (cellular response to inorganic substance), GO:0071248 (cellular response to metal ion), GO:0010039 (response to iron ion), GO:0071281 (cellular response to iron ion)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)
Protein: Polyglycine hydrolase (A0A0A7LRQ7)
Organism: Epicoccum sorghinum (Endophyte fungus) (Phoma sorghina)
Function: Serine-type endopeptidase that cleaves Gly-Gly bonds in the polyglycine linker of host plant class IV chitinases to disrupt their chitin-binding, and thereby plays a role in lowering the defense responses of the host to the fungus. Degrades Z.mays Endochitinase A (CHIA). Degrades Z.mays Endochitinase B (CHIB) . Has no activity on Z.mays CHIA following CHIA cleavage by fungalysin
Subcellular location: Secreted
InterPro domains:
- IPR050491: Beta-lactamase AmpC-like (family) [319-578]
- IPR012338: Beta-lactamase/transpeptidase-like (homologous_superfamily) [291-605]
- IPR049511: Polyglycine hydrolase-like, tandem repeat (repeat) [46-289]
- IPR001466: Beta-lactamase-related (domain) [319-577]
Sequence:
MYTSRLLLSNLASCLSLATLVASFPANQQDLTFAKRNGTFEQSVFYGLTGPEVEAKLAKLKADGYRPTSLNIHGSTSDAKYAGIWTKQTGDDFETILGANKTVYDAWLDSHKAQGYVSTHVSATGGSSDALFAGVMEKVPSVANWIQVCGLDNPYAYANATIDEPMYIKGVSMYGAPNERQYCILGHENLVNYQQTVFYQTDYFKKDYAKLLQSETSKRHWRPVFIDLSEDLLPTPIFDDTSVGQWVARTDLSASELEAEIAAQKAKNLYAVHIAGAGSKGSKYAVLFAEHLSPLERKWTVTGEVTGFKTNDVVAKDMDAVMEEFMKKNSVRQAQVAASVNGTVVAERSYTWAESDRAVVKPTDKFGLGSVSKMFTYAATTNLLNEGLLNHTTRVYPFLGMNNPADNRSLDITVDHLLQHTAGYNRDIKPDIGFIFRNIALERNQTTPVSLRELIEYVYEQPLDFTPGTDSVYSNYGTMLLSYLIANITGESFNSYIHKNVLNGLDVELYPTSPELNANNPIVQETKYTFYPAQDPASTKQVSNANGGDGSIREEAIGAFGLRASASTISQFLANHAAYDIGPRQAYTYRDGTIVGSRAFAQSQDLIDWSLILNTREYESEQKWEQLVFGPISQWYKYALAE
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016810 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds), GO:0016811 (hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0016999 (antibiotic metabolic process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0017000 (antibiotic biosynthetic process), GO:0044249 (cellular biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)
Protein: Picrinine-N-methytransferase (A0A075D657)
Organism: Vinca minor (Common periwinkle)
Function: S-adenosyl-L-methionine-dependent N-methyltransferase involved in the biosynthesis of biologically active monoterpenoid indole alkaloids (MIAs) natural products including vindoline . Catalyzes the conversion of picrinine to N- methylpicrinine (ervincine). Accepts also, with low efficiency, 21-hydroxycyclolochnericine and norajmaline as substrates
Subcellular location: Cytoplasm, cytosol
InterPro domains:
- IPR013216: Methyltransferase type 11 (domain) [104-201]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [15-320]
- IPR025774: Picrinine-N-methytransferase-like (family) [24-322]
- IPR050447: Erg6/SMT methyltransferase (family) [32-297]
Sequence:
MYTCSIIIYILTFWQLSKIKKQVAAAEKQVMTVTEKQEAVAEFYDKSTDAWEVFFGEHLHDGFYEPGTTATIPGSKVAVVRMIDELLRFAGISDDPEKKPKTMLDVGCGLGGTCLHVAKKYDIKCTGITISPEQVKCAQDLAATQGLESKVSFDVGDALDMPYKDGTFDLVFTIQCIEHIQDKEKFIREMVRVAAPGAPVVIAGYAARNLSPSEESLKPEEKMVLEKICDHIVLSWLCSTGDYVKWLTPLPVQDIKVWDLTQNITPFYPLCIKEAFTWKSFTSLLKMGGWSAIKVVFAVKMMAMAAEEGLLKFAAVTCRKSK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008168 (methyltransferase activity), GO:0008171 (O-methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009058 (biosynthetic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901615 (organic hydroxy compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0016125 (sterol metabolic process), GO:1901617 (organic hydroxy compound biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008202 (steroid metabolic process), GO:0016126 (sterol biosynthetic process), GO:0006694 (steroid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)