Protein: Lysine-specific histone demethylase (A0A8I5KRX9)
Organism: Homo sapiens (Human)
Function: Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity
Subcellular location: Nucleus
InterPro domains:
- IPR050281: Flavin monoamine oxidase and related enzymes (family) [190-601]
- IPR017366: Lysine-specific histone demethylase (family) [24-744]
- IPR002937: Amine oxidase (domain) [288-730]
- IPR036188: FAD/NAD(P)-binding domain superfamily (homologous_superfamily) [276-746]
- IPR036388: Winged helix-like DNA-binding domain superfamily (homologous_superfamily) [172-276]
- IPR009057: Homedomain-like superfamily (homologous_superfamily) [171-277]
- IPR007526: SWIRM domain (domain) [174-273]
Sequence:
MLSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSPLFFCSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0036094 (small molecule binding), GO:0097159 (organic cyclic compound binding), GO:0003712 (transcription coregulator activity), GO:0032451 (demethylase activity), GO:0016491 (oxidoreductase activity), GO:1901363 (heterocyclic compound binding), GO:0003682 (chromatin binding), GO:0043167 (ion binding), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0008134 (transcription factor binding), GO:1901265 (nucleoside phosphate binding), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0002039 (p53 binding), GO:0043168 (anion binding), GO:0140457 (protein demethylase activity), GO:0003713 (transcription coactivator activity), GO:0003676 (nucleic acid binding), GO:0000166 (nucleotide binding), GO:0019899 (enzyme binding), GO:0030374 (nuclear receptor coactivator activity), GO:0032452 (histone demethylase activity), GO:0050660 (flavin adenine dinucleotide binding), GO:0140297 (DNA-binding transcription factor binding), GO:0003723 (RNA binding), GO:0043425 (bHLH transcription factor binding), GO:0032453 (histone H3K4 demethylase activity), GO:0032454 (histone H3K9 demethylase activity), GO:0061629 (RNA polymerase II-specific DNA-binding transcription factor binding), GO:0016922 (nuclear receptor binding), GO:0050681 (nuclear androgen receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0009987 (cellular process), GO:0008152 (metabolic process), GO:0048519 (negative regulation of biological process), GO:0023057 (negative regulation of signaling), GO:0051240 (positive regulation of multicellular organismal process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0051239 (regulation of multicellular organismal process), GO:0070988 (demethylation), GO:0009892 (negative regulation of metabolic process), GO:0050793 (regulation of developmental process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0051716 (cellular response to stimulus), GO:0023051 (regulation of signaling), GO:0048523 (negative regulation of cellular process), GO:0009628 (response to abiotic stimulus), GO:0019222 (regulation of metabolic process), GO:0032879 (regulation of localization), GO:0071704 (organic substance metabolic process), GO:0009893 (positive regulation of metabolic process), GO:0065009 (regulation of molecular function), GO:0006950 (response to stress), GO:0051094 (positive regulation of developmental process), GO:0048585 (negative regulation of response to stimulus), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0009968 (negative regulation of signal transduction), GO:0006482 (protein demethylation), GO:0060548 (negative regulation of cell death), GO:0009314 (response to radiation), GO:0080134 (regulation of response to stress), GO:0009966 (regulation of signal transduction), GO:0060255 (regulation of macromolecule metabolic process), GO:0051098 (regulation of binding), GO:2001021 (negative regulation of response to DNA damage stimulus), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0071214 (cellular response to abiotic stimulus), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0051962 (positive regulation of nervous system development), GO:0008284 (positive regulation of cell population proliferation), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0045597 (positive regulation of cell differentiation), GO:0010648 (negative regulation of cell communication), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0044093 (positive regulation of molecular function), GO:1901564 (organonitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0044092 (negative regulation of molecular function), GO:0060341 (regulation of cellular localization), GO:0019538 (protein metabolic process), GO:0080135 (regulation of cellular response to stress), GO:0031324 (negative regulation of cellular metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:2000026 (regulation of multicellular organismal development), GO:0104004 (cellular response to environmental stimulus), GO:0042127 (regulation of cell population proliferation), GO:0010941 (regulation of cell death), GO:0031325 (positive regulation of cellular metabolic process), GO:0010646 (regulation of cell communication), GO:0033554 (cellular response to stress), GO:0051090 (regulation of DNA-binding transcription factor activity), GO:0009889 (regulation of biosynthetic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0080090 (regulation of primary metabolic process), GO:0033143 (regulation of intracellular steroid hormone receptor signaling pathway), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051100 (negative regulation of binding), GO:0051246 (regulation of protein metabolic process), GO:0043069 (negative regulation of programmed cell death), GO:0043067 (regulation of programmed cell death), GO:1902275 (regulation of chromatin organization), GO:0050769 (positive regulation of neurogenesis), GO:0043393 (regulation of protein binding), GO:0010468 (regulation of gene expression), GO:1902230 (negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage), GO:2000177 (regulation of neural precursor cell proliferation), GO:0043412 (macromolecule modification), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0010212 (response to ionizing radiation), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0051091 (positive regulation of DNA-binding transcription factor activity), GO:0071478 (cellular response to radiation), GO:0009416 (response to light stimulus), GO:0051052 (regulation of DNA metabolic process), GO:0006974 (cellular response to DNA damage stimulus), GO:0060284 (regulation of cell development), GO:0043518 (negative regulation of DNA damage response, signal transduction by p53 class mediator), GO:0010720 (positive regulation of cell development), GO:2000179 (positive regulation of neural precursor cell proliferation), GO:0006325 (chromatin organization), GO:2001234 (negative regulation of apoptotic signaling pathway), GO:0032880 (regulation of protein localization), GO:1902532 (negative regulation of intracellular signal transduction), GO:0051960 (regulation of nervous system development), GO:2001233 (regulation of apoptotic signaling pathway), GO:2001020 (regulation of response to DNA damage stimulus), GO:0043433 (negative regulation of DNA-binding transcription factor activity), GO:0036211 (protein modification process), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0051101 (regulation of DNA binding), GO:0051247 (positive regulation of protein metabolic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0051253 (negative regulation of RNA metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:1902531 (regulation of intracellular signal transduction), GO:0042981 (regulation of apoptotic process), GO:0000018 (regulation of DNA recombination), GO:0043392 (negative regulation of DNA binding), GO:0031445 (regulation of heterochromatin formation), GO:2001141 (regulation of RNA biosynthetic process), GO:2001243 (negative regulation of intrinsic apoptotic signaling pathway), GO:1901797 (negative regulation of signal transduction by p53 class mediator), GO:0006338 (chromatin remodeling), GO:0009411 (response to UV), GO:1902692 (regulation of neuroblast proliferation), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0008214 (protein dealkylation), GO:0050767 (regulation of neurogenesis), GO:0043516 (regulation of DNA damage response, signal transduction by p53 class mediator), GO:1902166 (negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator), GO:0060765 (regulation of androgen receptor signaling pathway), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0031401 (positive regulation of protein modification process), GO:0120261 (regulation of heterochromatin organization), GO:2001242 (regulation of intrinsic apoptotic signaling pathway), GO:1901796 (regulation of signal transduction by p53 class mediator), GO:0031399 (regulation of protein modification process), GO:0006282 (regulation of DNA repair), GO:0071479 (cellular response to ionizing radiation), GO:0043066 (negative regulation of apoptotic process), GO:0140861 (DNA repair-dependent chromatin remodeling), GO:0006355 (regulation of DNA-templated transcription), GO:0010332 (response to gamma radiation), GO:0071482 (cellular response to light stimulus), GO:1902229 (regulation of intrinsic apoptotic signaling pathway in response to DNA damage), GO:0002052 (positive regulation of neuroblast proliferation), GO:0032091 (negative regulation of protein binding), GO:1902165 (regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator), GO:1902254 (negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903322 (positive regulation of protein modification by small protein conjugation or removal), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0090308 (regulation of DNA methylation-dependent heterochromatin formation), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:1902253 (regulation of intrinsic apoptotic signaling pathway by p53 class mediator), GO:1903320 (regulation of protein modification by small protein conjugation or removal), GO:2000779 (regulation of double-strand break repair), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:0010569 (regulation of double-strand break repair via homologous recombination), GO:0034644 (cellular response to UV), GO:0071480 (cellular response to gamma radiation), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0031398 (positive regulation of protein ubiquitination), GO:0000122 (negative regulation of transcription by RNA polymerase II), GO:0031396 (regulation of protein ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)