Protein: DNA-directed RNA polymerase II subunit RPB9 (Q32P73)
Organism: Bos taurus (Bovine)
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2I/RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template
Subcellular location: Nucleus, nucleolus
InterPro domains:
- IPR012164: DNA-directed RNA polymerase subunit/transcription factor S (family) [14-125]
- IPR001222: Zinc finger, TFIIS-type (domain) [82-125]
- IPR001529: DNA-directed RNA polymerase II subunit RPB9-like, zinc ribbon (domain) [14-68]
- IPR034012: Pol II subunit B9, C-terminal zinc ribbon (domain) [74-124]
- IPR019761: DNA-directed RNA polymerase M, 15kDa subunit, conserved site (conserved_site) [16-42]
Sequence:
MEPDGTYEPGIVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0043167 (ion binding), GO:0016740 (transferase activity), GO:0005515 (protein binding), GO:0140098 (catalytic activity, acting on RNA), GO:0043169 (cation binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0046872 (metal ion binding), GO:0097747 (RNA polymerase activity), GO:0016779 (nucleotidyltransferase activity), GO:0046914 (transition metal ion binding), GO:0034062 (5'-3' RNA polymerase activity), GO:0003899 (DNA-directed 5'-3' RNA polymerase activity), GO:0008270 (zinc ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006725 (cellular aromatic compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0010467 (gene expression), GO:0018130 (heterocycle biosynthetic process), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0016070 (RNA metabolic process), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0006351 (DNA-templated transcription), GO:0006366 (transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:1902494 (catalytic complex), GO:0140513 (nuclear protein-containing complex), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0140535 (intracellular protein-containing complex), GO:0030880 (RNA polymerase complex), GO:0043233 (organelle lumen), GO:0055029 (nuclear DNA-directed RNA polymerase complex), GO:1990234 (transferase complex), GO:0016591 (RNA polymerase II, holoenzyme), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000428 (DNA-directed RNA polymerase complex), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0061695 (transferase complex, transferring phosphorus-containing groups), GO:0005665 (RNA polymerase II, core complex), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)