Protein: Transcription factor Clamp (Q9V9N4)
Organism: Drosophila melanogaster (Fruit fly)
Function: Transcription factor involved in X-chromosome dosage compensation in males, the process by which transcription of the single X chromosome in the male is elevated. Binds to the DNA sequence (GA)n. Clamp-binding promotes nucleosome depletion and chromatin accessibility, thereby allowing access to other transcription factors. Specifically binds to cis-acting elements on the X-chromosome named chromatin entry sites and promotes recruitment of the male-specific lethal (MSL) histone acetyltransferase complex, which associates with actively transcribed genes on the male X-chromosome to upregulate their expression. Mechanistically, acts by promoting chromatin accessibility at chromatin entry sites, facilitating DNA-binding of msl-2, followed by MSL complex recruitment. In addition to dosage compensation, also involved in zygotic genome activation (ZGA), a critical event in early embryonic development during which the developmental control passes from maternally provided mRNAs to the expression of the zygotic genome after fertilization. Maternally-provided protein cooperates with Zelda (zld) to activate zygotic transcription by increasing chromatin accessibility at promoters of specific genes and facilitate zld occupancy at a subset of key embryonic promoters. Also acts as an activator of gypsy chromatin insulator function by promoting binding of Cp190 to chromatin
Subcellular location: Nucleus. Chromosome. Note=Enriched at X-chromosome MSL- response elements (MREs) compared with autosomal MREs . On polytene chromosomes, localizes to many sites throughout the genomes of both males and females
InterPro domains:
- IPR036236: Zinc finger C2H2 superfamily (homologous_superfamily) [125-518]
- IPR013087: Zinc finger C2H2-type (domain) [127-522]
Sequence:
MEDLTKNIIFTNAINGQPATIQYQTADGTILKQPKIEGQKTEQQPTFYYTTNGNGGTVNLAQLATTDDNKTCYIAQPVGGYNYALVNGMPLNQGAALGIATVDAQGRIQIVNQNKPIAANTISNISFKCDVCSDMFPHLALLNAHKRMHTDGEQQQQQQHNAQAGGDSIAVVSAQGLVQAQNIIGNGQMGQIQIVSSDTLEPVQQSVMQQQQHESKASKCINCGSSMLQQSKRKGPKQVRCESCMQAEQTAQQQQQLFVAQDGQMAHPVQIISTTPQAQAQLQQIVAAQTGGTTPKREASSGSGHHPVKKRNSQQMTKCQKCNGSGVVLVGQHSHASHSGVGGSVKQSVTVKTENPSKPFSCNICGGLFSRYSSLWSHKKLHSGEKNYKCSICGLAFAKAVYLKNHARIHTGEKPYKCQTCGMQFSQSPHLKNHERTHSGERPYVCGVCDKGFARHATLWNHRRIHTGEKPYKCEICGSAFSQAAHLKNHAKVHSGEKPYKCEICSAAFADRFALKRHRGIHQKYGQTAPRQTSSDGMIVHKQEIPDMEDEAQQEVIIGGL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140110 (transcription regulator activity), GO:0097159 (organic cyclic compound binding), GO:0003700 (DNA-binding transcription factor activity), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0001216 (DNA-binding transcription activator activity), GO:0000981 (DNA-binding transcription factor activity, RNA polymerase II-specific), GO:0003677 (DNA binding), GO:0001228 (DNA-binding transcription activator activity, RNA polymerase II-specific), GO:0043565 (sequence-specific DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0048519 (negative regulation of biological process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0019222 (regulation of metabolic process), GO:0009791 (post-embryonic development), GO:0009892 (negative regulation of metabolic process), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0048523 (negative regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0048736 (appendage development), GO:0002165 (instar larval or pupal development), GO:0007552 (metamorphosis), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048513 (animal organ development), GO:0060255 (regulation of macromolecule metabolic process), GO:0035107 (appendage morphogenesis), GO:0031324 (negative regulation of cellular metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:0009887 (animal organ morphogenesis), GO:0031325 (positive regulation of cellular metabolic process), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0009886 (post-embryonic animal morphogenesis), GO:0080090 (regulation of primary metabolic process), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0035120 (post-embryonic appendage morphogenesis), GO:0048707 (instar larval or pupal morphogenesis), GO:0035114 (imaginal disc-derived appendage morphogenesis), GO:0010468 (regulation of gene expression), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0048737 (imaginal disc-derived appendage development), GO:0048569 (post-embryonic animal organ development), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0048563 (post-embryonic animal organ morphogenesis), GO:0007560 (imaginal disc morphogenesis), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0007444 (imaginal disc development), GO:0051253 (negative regulation of RNA metabolic process), GO:0045934 (negative regulation of nucleobase-containing compound metabolic process), GO:0031328 (positive regulation of cellular biosynthetic process), GO:0007478 (leg disc morphogenesis), GO:0007480 (imaginal disc-derived leg morphogenesis), GO:0007472 (wing disc morphogenesis), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:1902680 (positive regulation of RNA biosynthetic process), GO:0035218 (leg disc development), GO:1902679 (negative regulation of RNA biosynthetic process), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0045893 (positive regulation of DNA-templated transcription), GO:0045892 (negative regulation of DNA-templated transcription), GO:1903507 (negative regulation of nucleic acid-templated transcription), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II), GO:0000122 (negative regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)