Protein: Reverse gyrase 1 (Q97ZZ8)
Organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Function: Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process (Probable). Increases the linking number in steps of +1. Involved in homeostatic control of DNA topology in balance with type II topoisomerase 6 (TopoVI); levels of TopoVI are constant at 80 and 88 degrees Celsius and TopoVI is probably less active at 88 degrees (characterized enzyme is from S.shibatae B12), so reverse gyrase mediates most of the fine-tuning of DNA topology. Changes the DNA linking number step-by-step in a distributive manner. At low protein to DNA ratios mostly relaxes negatively supercoiled substrate, as ratios rise more positive supercoils are introduced. At 90 degrees Celsius introduces 19 positive supercoils into pTZ18R DNA (probably 2860 bp), less than TopR2. Relaxes negatively supercoiled DNA in the absence of ATP. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue (Probable). May be involved in DNA damage response (Probable). Its activity is inhibited by the DNA-binding protein 7d (Sso7d), suggesting that the Sso7d activity might counteract the overwinding effect of reverse gyrase (By similarity)
Subcellular location: Cytoplasm. Note=Upon UV irradiation up to 60% of the reverse gyrase activity becomes associated with DNA in vivo
InterPro domains:
- IPR003593: AAA+ ATPase domain (domain) [102-297]
- IPR040569: Reverse gyrase, zinc finger (domain) [6-46]
- IPR013826: DNA topoisomerase, type IA, central region, subdomain 3 (homologous_superfamily) [928-1054]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [85-315]
- IPR003602: DNA topoisomerase, type IA, DNA-binding domain (domain) [918-1194]
- IPR013824: DNA topoisomerase, type IA, central region, subdomain 1 (homologous_superfamily) [806-1237]
- IPR034142: Reverse gyrase, TOPRIM domain (domain) [619-797]
- IPR013497: DNA topoisomerase, type IA, central (domain) [749-1242]
- IPR023405: DNA topoisomerase, type IA, core domain (homologous_superfamily) [617-1240]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [98-240]
- IPR005736: Reverse gyrase (family) [7-1242]
- IPR003601: DNA topoisomerase, type IA, domain 2 (domain) [776-883]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [33-610]
- IPR006171: TOPRIM domain (domain) [619-785]
Sequence:
MTSINKVPPSIYTRSCPNCGGNISSQRLFNGSVCESCLKDDREFSNLSDLINILSENSNLKNLTQIRDVLEEYKKVEEIFGKLLNNSKPIGPQRSWTIRFLRGESFAIIAPPGLGKTTFGLIMSLYNATRNRKSIIIFPTRTLISQTVDKLAKFSELYSYSPRILYNKQSPTQTENILDQLKSGNFDIFISTNRFVIQNLSELSNIKFDFIFVDDVDAALKSGKSAKAILRLVGFTDEDIQTTMKLLRENIGEEEKFGKIQEIRESRLKDKIVIFSSATISRGNPILSSLMGFRPGSSVIYLRNIYDSYIDLTQTCKGQDFEECTLGTVIKLLKRLNDGTLIFVPIDKGAQYADYLASNLRDHGINVESVASSSISKLEKFERGEVSSLVGVATHYGVLVRGIDLPWRVKYSIFVGIPKFKFKIGEYMHPLALTRLLSLVYLVKNDDKVRGLLSYIRKRLRKISPAALAMLAKDIREGKIDDERLKEAYNLVNEYLKDNEFLKKVSDVGDLVIEGDYILMPDYLTYIQASGRTSRLYGANLTTGLSVLLIDNSRLFELLNKKLNLILDEVKWYSLDIDADKLGQVSLSDISAKITEERESLSKIKKEGNVESSSLSVKTTLFIVESPNKAKTISNFFSRPSTRSYGKLRVYETVLGDRVLIVAASGGHIYDLITEDESEKQDDNYVYGVLVKDSKFIPIYSTIKKCEKGHQIVKDLSQNKCPICGSRIVTDKTEVVDILRKLALEVDEVLIGTDPDTEGEKIAWDIYLAIRPFNGNIKRAEFHEVTRRAILNAIKNPREFNDNLVKSQIVRRIEDRWIGFKLSRKLQTEFWEQHCTSISKKNSKDEECKENRNLSAGRVQTPVLDWVVNRYQKYNENKKKYLIIESQDKSIFPFSVLALKKNGLSKNTQIIIHLEDINIKEEEFGPLPPYTTDTLLSDAANLLRIPASDTMRVAQDLFELGLITYHRTDSTRVSNVGISVAETYLKSKQVDISKIFRPRSWGEGGAHEAIRPTKPLDETMLKASIEQGDLELSKQLTFNHFRVYNLIFRRFITSQLPPLVVTKQIVRIRAYTKDNIELELDENKKEFVIGYKLKEGDEFRQTLQDAIYTLFRLYQPLDEKMKGKELSATITGTLNKSDVQLYTEGELISEMKSKQIGRPSTYAVIISTLKKRRYIIESKNLKKIIPTKLGMAVKEYLMENYKQIVSEKRTVKLLEKMNEVEEGKVDYLVLLKELYNEIQTIS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0006996 (organelle organization), GO:0090304 (nucleic acid metabolic process), GO:0006265 (DNA topological change), GO:0051276 (chromosome organization), GO:0071103 (DNA conformation change)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0000428 (DNA-directed RNA polymerase complex), GO:0061695 (transferase complex, transferring phosphorus-containing groups)