Protein: Endoribonuclease Dicer (A6JER2)
Organism: Rattus norvegicus (Rat)
Function: Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes
InterPro domains:
- IPR003100: PAZ domain (domain) [891-1066]
- IPR001650: Helicase, C-terminal domain-like (domain) [433-602]
- IPR014720: Double-stranded RNA-binding domain (domain) [1845-1910]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [39-588]
- IPR036085: PAZ domain superfamily (homologous_superfamily) [927-988]
- IPR048512: Dicer, platform domain (domain) [761-886]
- IPR005034: Dicer, dimerisation domain (domain) [630-722]
- IPR044441: Dicer, double-stranded RNA-binding domain (domain) [1826-1912]
- IPR006935: Helicase/UvrB, N-terminal (domain) [42-207]
- IPR038248: Dicer dimerisation domain superfamily (homologous_superfamily) [625-725]
- IPR048513: Dicer, partner-binding domain (domain) [268-366]
- IPR036389: Ribonuclease III, endonuclease domain superfamily (homologous_superfamily) [1291-1845]
- IPR000999: Ribonuclease III domain (domain) [1283-1843]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [40-243]
Sequence:
MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLSPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWSQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCDSCPSCPRILGLTASILNGKCDPDELEEKIQKLEKILKSGAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALDFINDCNVSVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDGAEADVHAVVDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMGCVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELVTRLHQYIFSHILRLEKPALEFQPAGAESAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISTCNSSLAESDNYCKHSTTVVPENAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSVDLTAINGLSYNKSLANGSYDLVNRDFCQGNQLTYFKQEIPVQPTTSYPIQNLYNYENQPTPSNECPLLSNKYLDGNANTSTSDGSPAGSPRPAMMTAVEALEGRTDSEQSPSVGHSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKQGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEDCEEEEEEELAWRAPKEEAEYEDDLLEYDQEHIQFIDSMLMGSGAFVKKIPLSPFSTSDSAYEWKMPKKASLGSVPFSSDLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSRDKASYPAQENSSSQQKSPSGSCAAAVSPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFENFEKKINYIFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPLVPNS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:1901363 (heterocyclic compound binding), GO:0016787 (hydrolase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0097159 (organic cyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0019904 (protein domain specific binding), GO:0004518 (nuclease activity), GO:0003723 (RNA binding), GO:0004540 (ribonuclease activity), GO:0003725 (double-stranded RNA binding), GO:0061980 (regulatory RNA binding), GO:0004521 (RNA endonuclease activity), GO:0004519 (endonuclease activity), GO:0016891 (RNA endonuclease activity, producing 5'-phosphomonoesters), GO:0016893 (endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters), GO:0035197 (siRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0042592 (homeostatic process), GO:0065007 (biological regulation), GO:0050896 (response to stimulus), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0009605 (response to external stimulus), GO:0009628 (response to abiotic stimulus), GO:0042221 (response to chemical), GO:0019222 (regulation of metabolic process), GO:0051239 (regulation of multicellular organismal process), GO:0048878 (chemical homeostasis), GO:0050793 (regulation of developmental process), GO:0019725 (cellular homeostasis), GO:0040017 (positive regulation of locomotion), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0051716 (cellular response to stimulus), GO:0006950 (response to stress), GO:0007568 (aging), GO:0051094 (positive regulation of developmental process), GO:0007154 (cell communication), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:0045597 (positive regulation of cell differentiation), GO:2000145 (regulation of cell motility), GO:0006979 (response to oxidative stress), GO:0070482 (response to oxygen levels), GO:0010714 (positive regulation of collagen metabolic process), GO:0030155 (regulation of cell adhesion), GO:0033500 (carbohydrate homeostasis), GO:1901700 (response to oxygen-containing compound), GO:0045785 (positive regulation of cell adhesion), GO:0010712 (regulation of collagen metabolic process), GO:0010033 (response to organic substance), GO:2000147 (positive regulation of cell motility), GO:0009891 (positive regulation of biosynthetic process), GO:0009991 (response to extracellular stimulus), GO:0010632 (regulation of epithelial cell migration), GO:0055082 (intracellular chemical homeostasis), GO:0010035 (response to inorganic substance), GO:2000026 (regulation of multicellular organismal development), GO:0042127 (regulation of cell population proliferation), GO:0070887 (cellular response to chemical stimulus), GO:0033554 (cellular response to stress), GO:0009889 (regulation of biosynthetic process), GO:0045595 (regulation of cell differentiation), GO:0031668 (cellular response to extracellular stimulus), GO:0071496 (cellular response to external stimulus), GO:0008284 (positive regulation of cell population proliferation), GO:0034599 (cellular response to oxidative stress), GO:0010811 (positive regulation of cell-substrate adhesion), GO:0009743 (response to carbohydrate), GO:0034097 (response to cytokine), GO:0032967 (positive regulation of collagen biosynthetic process), GO:0048145 (regulation of fibroblast proliferation), GO:0071453 (cellular response to oxygen levels), GO:0010810 (regulation of cell-substrate adhesion), GO:0071310 (cellular response to organic substance), GO:0062197 (cellular response to chemical stress), GO:0060284 (regulation of cell development), GO:0010720 (positive regulation of cell development), GO:0030334 (regulation of cell migration), GO:0050678 (regulation of epithelial cell proliferation), GO:0050679 (positive regulation of epithelial cell proliferation), GO:0032965 (regulation of collagen biosynthetic process), GO:0010594 (regulation of endothelial cell migration), GO:0031667 (response to nutrient levels), GO:0042593 (glucose homeostasis), GO:0036293 (response to decreased oxygen levels), GO:0010634 (positive regulation of epithelial cell migration), GO:0030335 (positive regulation of cell migration), GO:0042542 (response to hydrogen peroxide), GO:0048146 (positive regulation of fibroblast proliferation), GO:1901701 (cellular response to oxygen-containing compound), GO:0001678 (intracellular glucose homeostasis), GO:0031669 (cellular response to nutrient levels), GO:0000302 (response to reactive oxygen species), GO:0030856 (regulation of epithelial cell differentiation), GO:0001938 (positive regulation of endothelial cell proliferation), GO:0090649 (response to oxygen-glucose deprivation), GO:0071322 (cellular response to carbohydrate stimulus), GO:0071333 (cellular response to glucose stimulus), GO:1901550 (regulation of endothelial cell development), GO:0001952 (regulation of cell-matrix adhesion), GO:0010595 (positive regulation of endothelial cell migration), GO:0034284 (response to monosaccharide), GO:0045601 (regulation of endothelial cell differentiation), GO:0071345 (cellular response to cytokine stimulus), GO:0036294 (cellular response to decreased oxygen levels), GO:0034612 (response to tumor necrosis factor), GO:0001936 (regulation of endothelial cell proliferation), GO:0001954 (positive regulation of cell-matrix adhesion), GO:0034614 (cellular response to reactive oxygen species), GO:0070301 (cellular response to hydrogen peroxide), GO:0090650 (cellular response to oxygen-glucose deprivation), GO:0071326 (cellular response to monosaccharide stimulus), GO:1905562 (regulation of vascular endothelial cell proliferation), GO:1905564 (positive regulation of vascular endothelial cell proliferation), GO:1903140 (regulation of establishment of endothelial barrier), GO:0009746 (response to hexose), GO:0071356 (cellular response to tumor necrosis factor), GO:0071331 (cellular response to hexose stimulus), GO:0009749 (response to glucose)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0150034 (distal axon), GO:0042995 (cell projection), GO:0030427 (site of polarized growth), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005783 (endoplasmic reticulum), GO:0120025 (plasma membrane bounded cell projection), GO:0097447 (dendritic tree), GO:0043229 (intracellular organelle), GO:0030426 (growth cone), GO:0043227 (membrane-bounded organelle), GO:0005793 (endoplasmic reticulum-Golgi intermediate compartment), GO:0043005 (neuron projection), GO:0043231 (intracellular membrane-bounded organelle), GO:0030425 (dendrite), GO:0030424 (axon)