Protein: Reverse gyrase (P74759)
Organism: Saccharolobus shibatae (strain ATCC 51178 / DSM 5389 / JCM 8931 / NBRC
Function: Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. Increases the linking number in steps of +1. In vitro requires high concentrations to supercoil negatively supercoiled DNA, relaxes plasmid DNA first; DNA single-strand binding protein (SSB) from S.solfataricus strain P2 stimulates positive supercoiling. SSB stimulates DNA- binding by reverse gyrase, and thus all subsequent steps . Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'- phosphotyrosyl)-enzyme intermediate (Probable). May be involved in DNA damage response (Probable). Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection
Subcellular location: Cytoplasm
InterPro domains:
- IPR040569: Reverse gyrase, zinc finger (domain) [1-40]
- IPR013826: DNA topoisomerase, type IA, central region, subdomain 3 (homologous_superfamily) [866-992]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [84-285]
- IPR003602: DNA topoisomerase, type IA, DNA-binding domain (domain) [856-1109]
- IPR013824: DNA topoisomerase, type IA, central region, subdomain 1 (homologous_superfamily) [763-1152]
- IPR034142: Reverse gyrase, TOPRIM domain (domain) [580-755]
- IPR013497: DNA topoisomerase, type IA, central (domain) [707-1157]
- IPR023405: DNA topoisomerase, type IA, core domain (homologous_superfamily) [578-1154]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [91-157]
- IPR005736: Reverse gyrase (family) [1-1158]
- IPR003601: DNA topoisomerase, type IA, domain 2 (domain) [734-822]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [45-578]
- IPR006171: TOPRIM domain (domain) [580-743]
Sequence:
MINVMYKNSCPNCGGDISADRLLNGLPCETCLPYINGIDGIDHISKVKALYNILLDNDKIKNYWNLYYNITTFETVFKYFKDKTGYEPWSLQKLWLRRLVSNQSFTMSAPTGLGKTTTLMTYSVFIGQDVVYIVPTKSLMEQVCKRLEKLGAQVSCGKVDQRKVSVITISYLNKNADSITSYKPNFVAIDDADAVIKSGKTTDRLVSLLGIPNDVYESAIQLIRLRNKYYFSNEFTEEIKEKIRELELKIAEFKDKISQLVIASATIRPKGIKQKALRLLTGFEPSSIQLYARNIIDTYTDNLDLSIIKELGSGGLILVSKEYGRSRLNEIKKYVEDLGFNAKLAISGRKFLDDFSQGKVDILVGSASYYGVAVRGIDEPKRLKYVIYYGVPKIKAKLFDALSNPFTLLRVGKMIGVNFSELQNKILVLSPSEAQLLKFSIIKGETINYQKLEQLRQELLYYISLVKDKLKEIGEETLISDNFVIAKQNTNYYIIYPDMITYLQGSGRASRLYNGGLTLGFSIILVDDKHIFEILKKKMQKLFPNTNFTSLSNINLSEIKTKLEESRKEEGNRVHFNISTGLLIVESPTKAKTIAKMFSRPSVRVINKVPVYETIIVDGNQIYVLDVVASKGHIVDLTLEDIGYYGIKIEYSGIIKPYYDLIKKCLDCNKTFSIASDKCPYCGSTNVQTAQTTINLLRELALSVDKVFIASDPDTEGEKIAYDLASFLSPYNSNIYRITYHEITKKAILEALRNPMKINTNLVMSQIVRRIEDRWIGFTLSNLLKTKFNGHNHGAGRVQTPVLGWIVDKTIKYKSAMGYVVYIDIAGYPIKMHFSERKKMEEYINNLQVVKIEKIFEEKILLSPLPPFTTDTLLIEANMKYKLPANLVMKIAQDLFEAGLITYHRTDSTHISSVGIEIAKEYLQKQGLIKDFVPRSWESSEEGAHEAIRPTRAIDVNELIQEIEENPYKYSIRFSKLHFLIYDLIFRRFMASQMSHAVGTKSRYLIKLNKNDNINAELLSNAEGGFIKVYPVKVYNLPLGEVKPKVNTGKGSSEQLLSYSDVISLMKSKGIGRPSTYAKTIENLVRHGYIVSSKRKSYLIATNRGISAYQFLSSKFYDLVSEGTTAKLMSKLDDIALSKLSASTVLLEIFSEISTLVNPLKSEQNV
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding), GO:0042802 (identical protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0006259 (DNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0006310 (DNA recombination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:1902494 (catalytic complex), GO:0140535 (intracellular protein-containing complex), GO:0030880 (RNA polymerase complex), GO:1990234 (transferase complex), GO:0000428 (DNA-directed RNA polymerase complex), GO:0061695 (transferase complex, transferring phosphorus-containing groups)