Protein: Histone-lysine N-methyltransferase KMT5B (Q5U3H2)
Organism: Danio rerio (Zebrafish) (Brachydanio rerio)
Function: Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (By similarity). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Plays a role in myogenesis by regulating the expression of target genes, such as EID3. Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity)
Subcellular location: Nucleus. Chromosome. Note=Associated with pericentric heterochromatin
InterPro domains:
- IPR039977: Histone-lysine N-methyltransferase Suv4-20/Set9 (family) [7-489]
- IPR025790: Suv4-20 family, animal (family) [14-336]
- IPR041938: Histone-lysine N-methyltransferase, N-terminal domain (homologous_superfamily) [61-165]
- IPR046341: SET domain superfamily (homologous_superfamily) [156-310]
- IPR001214: SET domain (domain) [168-289]
- IPR044424: KMT5B , SET domain (domain) [167-310]
Sequence:
MGESKNMVLNGRRHGRKFSSNQPVSKSRLQNTQRSHLRQNKGSPSVRRCSRRCGGAPPEAERRHVPSSGMTAKELCEYDDLSTSLILDPYLGFQTHKMNTRFRPIKGRQRELREIIELFKKHDNLEKAFQALTSGDWTRHHFLNKTKSQEKLFKAHVFVYLRMFASDSGFEILSCNRYSSEQNGAKIVATKDWKRNDKIEHLVGCIAELSPSEERMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKVLRDIEPGEEISCYYGDGFFGENNEFCECYTCERRGTGAFKSKPGLPVEAPVINSKYGLRETDKRLNRLKKLGESCRNSDSQSVSSNAEADSQEPTTVQTSLRKRTSQSCVKKHGEAKAVTRQTLSSTPSSTSSSKRSQANISSLPKRLKSKPTQTLSKGRRRCRGLWTKGSSRVSASGNLKESSRRDTDRRRSASKGSVARSSENNRSSSKGASPCKDSTLCPYRTRRSTRTSLGAQGAEGTEASNHPASPSIVLKSEPGEFIPVTLGHQMSTPSLDSSCPKKGTCPRRRRTVKQEDSYGESFVQEGVPDLRHAVRAADVADCGKVVLGLPDRHQHYNGSSKSSKALRRGKGKKKRQITRYDAQLILQNNSGIPKITLRRRRDSSSSKNEPRETSSSSSSKISIKFSKEHEKDRSSSYVAKLNNGFSHGPHSSSTKLKIQLKREEDPASLHRTYPEDVTLGIVQRDAADVLDHKAAAQVGQDMEVESMSSEDDDDDYFDNEDDFIPLPPAKRLRLIVGKDSIDIDISSRRREDQSLRLNA
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008276 (protein methyltransferase activity), GO:0042054 (histone methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0140939 (histone H4 methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0050793 (regulation of developmental process), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0044238 (primary metabolic process), GO:0051239 (regulation of multicellular organismal process), GO:0071704 (organic substance metabolic process), GO:0040008 (regulation of growth), GO:1901564 (organonitrogen compound metabolic process), GO:0040014 (regulation of multicellular organism growth), GO:0019538 (protein metabolic process), GO:0043414 (macromolecule methylation), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0048638 (regulation of developmental growth), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006479 (protein methylation), GO:0008213 (protein alkylation), GO:0018022 (peptidyl-lysine methylation), GO:0016571 (histone methylation), GO:0018193 (peptidyl-amino acid modification), GO:0016570 (histone modification), GO:0034968 (histone lysine methylation), GO:0018205 (peptidyl-lysine modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0000785 (chromatin), GO:0043229 (intracellular organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome)