Protein: Serine/threonine-protein kinase ATG1 (Q5A649)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Serine/threonine protein kinase involved in the cytoplasm to vacuole transport (Cvt) and found to be essential in autophagy, where it is required for the formation of autophagosomes. Involved in the clearance of protein aggregates which cannot be efficiently cleared by the proteasome. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER- associated degradation (ERAD) substrates. Plays a key role in ATG9 and ATG23 cycling through the pre-autophagosomal structure and is necessary to promote ATG18 binding to ATG9 through phosphorylation of ATG9. Catalyzes phosphorylation of ATG4, decreasing the interaction between ATG4 and ATG8 and impairing deconjugation of PE-conjugated forms of ATG8
Subcellular location: Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein
InterPro domains:
- IPR045269: Serine/threonine-protein kinase Atg1-like (family) [48-516]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [200-212]
- IPR048941: ATG1-like, MIT domain 2 (domain) [764-830]
- IPR000719: Protein kinase domain (domain) [58-354]
- IPR022708: Serine/threonine-protein kinase Atg1-like, tMIT domain (domain) [624-744]
- IPR017441: Protein kinase, ATP binding site (binding_site) [64-87]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [56-410]
Sequence:
MIPQSNPTAQRRSGDAQNTNNVAPNSVATTTDTALTASTQSGSSSNNANKKLEYIGVYKIGPEIGKGSFATVYKCIDTTNNKAVAIKSVYRSKLKSKKLLENLEIEIQILKSMKHPHIVGLLDYKQTTSYFHLVMDYCSMGDLSYFIRRRNNLVKSHPVISSLLHCYPSPEGSHGLNEVLVLHFLRQLSSALQFLRDKSLVHRDIKPQNLLLCPPVHSKQEFIDGEFVGMWELPILKIADFGFARFLPSTSMAETLCGSPLYMAPEILRYEKYNAKADLWSVGAVLYEMTVGKPPFKAGNHIELLKNIEKANDKIKFPSAAQVPEPLKQLIRSLLKYNPTERISFNEFFNDSLITCDLDDNDQPLETSQMDENLFISEYISPIAPAERSQFFKEQKKNDSVVRSPSPTTATTATPRQDNVVQQMTKITSPVPDDFALSIARNSSEFNLKKDDMNLEKDYVVVEKRAVEVNALADELAHAGAGADAIPNSRKNSDVDQTNRLASSQQQTETASYRRSSSSGSQKRPSFSERRISLSLSPTNALTKAIGLASNRLFGLTTNSSHSNVSAIAEDDDSSTSNNDASSFSTVIPSTNNHVLLQKLNLATIGEPGTEFDLGSVSNLDEQILDKLELIANIANAVNLYADVKFSQIIPSPPSSDGIEDDTEMLPPKIIHMISQEGIGLYIKTLSLLGRAMDIAGQWWFEKYDAVHGERPSFETTVRINQIVQWIREKYNISLERLEFLKSKSDFTAEETIEDNEPNGTTRVQQAIFAAALGIARETALKELLRNSTDIECSYVTSIYMLLAILEDLEESDRQEVKKIIEKINSRLKNFMGK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0040007 (growth), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0009605 (response to external stimulus), GO:0051716 (cellular response to stimulus), GO:0030447 (filamentous growth), GO:0006950 (response to stress), GO:0009607 (response to biotic stimulus), GO:0007154 (cell communication), GO:0009991 (response to extracellular stimulus), GO:0031668 (cellular response to extracellular stimulus), GO:0044182 (filamentous growth of a population of unicellular organisms), GO:0036180 (filamentous growth of a population of unicellular organisms in response to biotic stimulus), GO:0042594 (response to starvation), GO:0071496 (cellular response to external stimulus), GO:0033554 (cellular response to stress), GO:0036170 (filamentous growth of a population of unicellular organisms in response to starvation), GO:0009267 (cellular response to starvation), GO:0031669 (cellular response to nutrient levels), GO:0031667 (response to nutrient levels)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0005773 (vacuole), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0005776 (autophagosome), GO:0043231 (intracellular membrane-bounded organelle)