Protein: Aurora kinase (E2RTQ7)
Organism: Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia)
Function: Involved in regulation of the cell cycle. Required for mitotic cell division and cytokinesis . Based on its localization to centrosomes and spindle microtubules, as well as to various cytoskeletal components such as the median body, parental attachment disk, and anterior and posterior-lateral paraflagellar dense rods, may coordinate reorganization and segregation of tubulin-containing structures during mitosis and cytokinesis. May regulate microtubule disassembly by phosphorylating cytoskeletal proteins leading to their destabilization. Phosphorylates EB1 at 'Ser-148' in vitro. Phosphorylates histone H3 in vitro
Subcellular location: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton. Nucleus membrane . Note=Non-phosphorylated form is exclusively localized to both nuclei in interphase trophozoites. At the beginning of mitosis, it is mostly in the nuclei and co-localizes with chromatin through telophase. Phosphorylated form co-localizes with centrin to centrosomes from prophase through cytokinesis. Phosphorylated form localizes also to the spindle microtubules surrounding each two nuclei and the spindle poles in metaphase. It is additionally dynamically localized to cytoskeletal structures including the median body, rim of the attachment disk, and anterior and posterior-lateral paraflagellar dense rods during different stages of mitosis. Phosphorylated form remains localized to microtubules of both spindles in anaphase through telophase as the spindles elongate to allow the four nuclei segregate to opposite poles of both spindles. Phosphorylated form localizes only to the gradually unfolding parental ventral attachment disk called pontoon later in telophase and cytokinesis, but not to the two nascent daughter disks dorsal to the disassembling parental disk and spindles. Phosphorylated form localizes to the median body in all stages of mitosis, but it is less prominent after anaphase. Neither non-phosphorylated nor phosphorylated form localizes to endoplasmic reticulum, lysosome-like peripheral vesicles, mitosomes or flagellar axonemes. Colocalizes with EB1 at the nuclear membrane and median bodies in interphase, at the spindle microtubules surrounding the chromosomes in metaphase, at the mitotic spindles in anaphase and telophase, and at the nuclear membranes in cytokinesis
InterPro domains:
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [139-151]
- IPR000719: Protein kinase domain (domain) [20-300]
- IPR030616: Aurora kinase-like (family) [12-309]
- IPR017441: Protein kinase, ATP binding site (binding_site) [26-49]
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [11-305]
Sequence:
MPQHLVPHTGTGKRTTIEDFEIGRFLGRGKYGLVYLAREQSSKLVVALKVLYKSYIKSERVEGQVRRELDIHLNVRHINIIRLYTWFQDETRVFLVLEVAPYGELYQRLQQFGKFPLPVVSKIIRDVAQAIQYLHRKNIFHRDLKAENILICKGKETKEHTDAHNSDDSISVHEHELVRMAHYTYKIADFGWSVHHPTHGGRRRTQCGTLDYLPPEVMLGQSYDKACDIWSLGALCYELICGTAPFYHDEIKITRQNIANVEYSFTKDFSPASKDFIQRMLIRSPEARISIEDILRHPFLRQTDHRSKVPK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:0006793 (phosphorus metabolic process), GO:0036211 (protein modification process), GO:0006796 (phosphate-containing compound metabolic process), GO:0043412 (macromolecule modification), GO:0016310 (phosphorylation), GO:0006468 (protein phosphorylation), GO:0046777 (protein autophosphorylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)