Protein: Trinucleotide repeat-containing gene 6B protein (Q8BKI2)
Organism: Mus musculus (Mouse)
Function: Plays a role in RNA-mediated gene silencing by both micro- RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA- dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffolding protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes
Subcellular location: Cytoplasm, P-body. Note=Mammalian P-bodies are also known as GW bodies (GWBs)
InterPro domains:
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [1619-1706]
- IPR032226: TNRC6, PABC binding domain (domain) [1338-1622]
- IPR019486: Argonaute hook domain (domain) [970-1093]
- IPR034925: TNRC6B, RNA recognition motif (domain) [1624-1706]
- IPR052068: GW182 domain-containing protein (family) [1168-1807]
- IPR035979: RNA-binding domain superfamily (homologous_superfamily) [1623-1699]
Sequence:
MQTNEGEVEEESSSQVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPCTAPGANPNNNAQVTGALLQSESGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSSLPGSTTSNKGKGSQCQAASSGNECNLGVWKSDPKAKSVQPPNSTSDSNNGLGNWRSTSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGTSRKGALETESSSSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNTSRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTVSGQSNSGNNGNNGKDREDSWKGASVPKPTGSKSDSWDNNNRSTGGSWNFGPQDNNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKSTGGWNDYKNNNSSNWGGGRADEKTPSSWNESSCKDQGWGGGRQPNQGWTSGKNGWGEEVDQVKNNNWESSANKPVSGWGEGGQNEIGTWGNGGNTNLASKGGWEDCKRSPAWNETGRQPNSWNKQHQQQQQPPPPQPEASGSWGGPPPPPQGNVRPSNSNWSSGPQPTTPKDDEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTGKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDAGASTTGWGNTPANAPNAMKPNSKSMQDGWGESDGPVTGARHPSWEEEDDGGVWNTAGSQGSTSSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVDKKFDVDKRTMNLGDFNDIMRKDRPGFRPPNSKDLGTTDSGPYFEKGGSHGLFGNSTAQSRGLHTPVQPLSSSPGLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQNQRKISQAVRQQQEQQLARMVSALQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLPTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEATMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTTTSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLTRPPPGLTNPKPASPWSSTAPRSVRGWGTQDSRIASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATEDEVSRFLAQAQPPTPAATPSAPATGWQSLETSQNQADPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGTSLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0065007 (biological regulation), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:2000241 (regulation of reproductive process), GO:0019222 (regulation of metabolic process), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0009892 (negative regulation of metabolic process), GO:0065008 (regulation of biological quality), GO:0050793 (regulation of developmental process), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0050794 (regulation of cellular process), GO:0009893 (positive regulation of metabolic process), GO:0021700 (developmental maturation), GO:0048523 (negative regulation of cellular process), GO:0048609 (multicellular organismal reproductive process), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:0007276 (gamete generation), GO:0051172 (negative regulation of nitrogen compound metabolic process), GO:0043487 (regulation of RNA stability), GO:2000145 (regulation of cell motility), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0060255 (regulation of macromolecule metabolic process), GO:0048608 (reproductive structure development), GO:0045137 (development of primary sexual characteristics), GO:0031324 (negative regulation of cellular metabolic process), GO:0071695 (anatomical structure maturation), GO:0009894 (regulation of catabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0048469 (cell maturation), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0045595 (regulation of cell differentiation), GO:0031323 (regulation of cellular metabolic process), GO:0009890 (negative regulation of biosynthetic process), GO:0007548 (sex differentiation), GO:0080090 (regulation of primary metabolic process), GO:0046661 (male sex differentiation), GO:0009896 (positive regulation of catabolic process), GO:0071695 (anatomical structure maturation), GO:0061458 (reproductive system development), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0051246 (regulation of protein metabolic process), GO:0043488 (regulation of mRNA stability), GO:0051248 (negative regulation of protein metabolic process), GO:0048477 (oogenesis), GO:0008406 (gonad development), GO:0046546 (development of primary male sexual characteristics), GO:0030334 (regulation of cell migration), GO:0010468 (regulation of gene expression), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0034248 (regulation of amide metabolic process), GO:0007292 (female gamete generation), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:0031331 (positive regulation of cellular catabolic process), GO:0010558 (negative regulation of macromolecule biosynthetic process), GO:0031327 (negative regulation of cellular biosynthetic process), GO:0051252 (regulation of RNA metabolic process), GO:0031329 (regulation of cellular catabolic process), GO:0010629 (negative regulation of gene expression), GO:0034249 (negative regulation of amide metabolic process), GO:0017148 (negative regulation of translation), GO:0061013 (regulation of mRNA catabolic process), GO:0061014 (positive regulation of mRNA catabolic process), GO:0016441 (post-transcriptional gene silencing), GO:0031047 (RNA-mediated gene silencing), GO:2000112 (regulation of cellular macromolecule biosynthetic process), GO:1900151 (regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay), GO:0006417 (regulation of translation), GO:1903311 (regulation of mRNA metabolic process), GO:2000113 (negative regulation of cellular macromolecule biosynthetic process), GO:1903313 (positive regulation of mRNA metabolic process), GO:0010608 (post-transcriptional regulation of gene expression), GO:0035194 (RNA-mediated post-transcriptional gene silencing), GO:0060211 (regulation of nuclear-transcribed mRNA poly(A) tail shortening), GO:0060213 (positive regulation of nuclear-transcribed mRNA poly(A) tail shortening), GO:1900153 (positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay), GO:0035195 (miRNA-mediated gene silencing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)