Protein: Bromodomain-containing protein 2 (Q7JJ13)
Organism: Mus musculus (Mouse)
Function: Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (By similarity). Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation. Also recognizes and binds acetylated non-histone proteins, such as STAT3 . Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin. In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6- methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (By similarity). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (By similarity)
Subcellular location: Nucleus. Chromosome. Note=Detected on chromatin and nucleosomes
InterPro domains:
- IPR001487: Bromodomain (domain) [71-454]
- IPR050935: Bromodomain-containing chromatin reader (family) [25-794]
- IPR036427: Bromodomain-like superfamily (homologous_superfamily) [59-459]
- IPR043508: Brdt, bromodomain, repeat I (domain) [73-179]
- IPR018359: Bromodomain, conserved site (conserved_site) [95-427]
- IPR027353: NET domain (domain) [630-712]
- IPR043509: Brdt, bromodomain, repeat II (domain) [348-449]
- IPR038336: NET domain superfamily (homologous_superfamily) [632-712]
Sequence:
MLQNVTPHKLPGEGNAGLLGLGPEAAAPGKRIRKPSLLYEGFESPTMASVPALQLAPANPPPPEVSNPKKPGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVVTIPKNSHKKGAKLAALQGSITSAHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAGPPLLAVSAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLSTVKRKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVSTALPPGLTKSSSESSSEESSSESSSEEEEEEEEDEDEEESESSDSEEERAHRLAELQEQLRAVHEQLAALSQGPISKPKRKREKKEKKKKRKAEKHRGRIGIDEDDKGPRAPRPPQPKKSKKAGGGGSNATTLSHPGFGTSGGSSNKLPKKSQKTAPPVLPTGYDSEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDFETLKPSTLRELERYVLSCLRKKPRKPYTIRKPVGKTKEELALEKKRELEKRLQDVSGQLNSTKKPPKKASEKTESSAQQVAVSRLSASSSSSDSSSSSSSSSSSDTSDSDSG
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003682 (chromatin binding), GO:0005515 (protein binding), GO:0042393 (histone binding), GO:0140030 (modification-dependent protein binding), GO:0140033 (acetylation-dependent protein binding), GO:0070577 (lysine-acetylated histone binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0019222 (regulation of metabolic process), GO:0048646 (anatomical structure formation involved in morphogenesis), GO:0035295 (tube development), GO:0048598 (embryonic morphogenesis), GO:0048729 (tissue morphogenesis), GO:0035148 (tube formation), GO:0009888 (tissue development), GO:0048731 (system development), GO:0060255 (regulation of macromolecule metabolic process), GO:0009790 (embryo development), GO:0035239 (tube morphogenesis), GO:0060562 (epithelial tube morphogenesis), GO:0007399 (nervous system development), GO:0021915 (neural tube development), GO:0060606 (tube closure), GO:0002009 (morphogenesis of an epithelium), GO:0060429 (epithelium development), GO:0016331 (morphogenesis of embryonic epithelium), GO:0010468 (regulation of gene expression), GO:0009792 (embryo development ending in birth or egg hatching), GO:0072175 (epithelial tube formation), GO:0001838 (embryonic epithelial tube formation), GO:0043009 (chordate embryonic development), GO:0001841 (neural tube formation), GO:0001843 (neural tube closure), GO:0014020 (primary neural tube formation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)