DNA sequencer
Comparison
Columns: Sequencer | Ion Torrent PGM | 454 GS FLX | HiSeq 2000 | SOLiDv4 | PacBio | Sanger 3730xl
Manufacturer | Ion Torrent (Life Technologies) | 454 Life Sciences (Roche) | Illumina | Applied Biosystems (Life Technologies) | Pacific Biosciences | Applied Biosystems (Life Technologies) | Sequencing Chemistry | Ion semiconductor sequencing | Pyrosequencing | Polymerase-based sequence-by-synthesis | Ligation-based sequencing | Phospholinked fluorescent nucleotides | Dideoxy chain termination | Amplification approach | Emulsion PCR | Emulsion PCR | Bridge amplification | Emulsion PCR | Single-molecule; no amplification | PCR | Data output per run | 100-200 Mb | 0.7 Gb | 600 Gb | 120 Gb | 100-500 Mb | 1.9∼84 Kb | Accuracy | 99% | 99.9% | 99.9% | 99.94% | 90% | 99.999% | Time per run | 2 hours | 24 hours | 3–10 days | 7–14 days | 2 hours | 20 minutes - 3 hours | Read length | 200-400 bp | 700 bp | 100x100 bp paired end | 50x50 bp paired end | 1,500-5,000 bp (avg) | 400-900 bp | Cost per run | $350 USD | $7,000 USD | $6,000 USD (30x human genome) | $4,000 USD | $300 USD | $4 USD (single read/reaction) | Cost per Mb | $1.00 USD | $10 USD | $0.07 USD | $0.13 USD | $2.00 USD | $2400 USD