Question: Which party experienced a result of Lost re-election Democratic-Republican hold?
SQL: SELECT "Party" FROM "1-2668352-16" WHERE "Result" = 'Lost re-election Democratic-Republican hold';

Question: How many districts have John C. Calhoun as the incumbent?
SQL: SELECT COUNT("District") FROM "1-2668352-16" WHERE "Incumbent" = 'John C. Calhoun';