user: Question: What's the total against for opposing team scotland at twickenham, london venue with a status of five nations?
Columns: ['Opposing Teams', 'Against', 'Date', 'Venue', 'Status']
Types: ['text', 'real', 'text', 'text', 'text']
Sample row: ['Ireland', 8.0, '21/01/1995', 'Lansdowne Road , Dublin', 'Five Nations']
SQL:

assistant: SELECT SUM("Against") FROM "Table" WHERE "Status" = 'five nations' AND "Venue" = 'twickenham, london' AND "Opposing Teams" = 'scotland';