Effects of Breaking Sitting on Human Behaviour and Metabolic Health

Study Overview
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Brief Summary
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This study primarily aimed to investigate effects of breaking up prolonged sitting with intermittent brisk walking in healthy young individuals on (1) post-trial human behaviours including energy intake and physical activity under free-living conditions and (2) cognitive performance in a simulated workplace environment.

Official Title
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Effects of Breaking Prolonged Sitting on 24-h Human Behaviours, Self-feelings, Metabolic Health and Cognition in Healthy Adults

Conditions
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Energy Balance, Physical Activity Level, Cognition, Metabolic Health

Intervention / Treatment
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* Behavioral: Prolonged sitting
* Behavioral: Breaking prolonged sitting


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Without current and any history of brain injury, neurological disorder, cardiometabolic related diseases, diabetes or cancers. Weight stable (not self-reported weight change ± 3 kg at least 3 months) Body mass index (BMI) ranging between 18.5 to 27 kg/m2 Can walk safely on a treadmill Exclusion Criteria: Smokers, post-menopausal female, and volunteers who used any medications that could influence metabolic and inflammatory responses.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Prolonged sitting<br>Participants sat on a chair during the trial in the laboratory. | Behavioral: Prolonged sitting<br>* In the laboratory, after consuming 1st meal, participants sat on a chair for 180 min. After 180 min, the 2nd meal was provided. After finishing the 2nd meal, participants sat on a chair for another 120 min. After data collection was completed in the laboratory, participants were asked to keep wearing the same pedometer and to record any food and liquid consumption for the rest of the day (until midnight) under free-living conditions.<br>|
| Experimental: Breaking prolonged sitting<br>Participants walked regularly during the trial in the laboratory. | Behavioral: Breaking prolonged sitting<br>* In the laboratory, after consuming 1st meal, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, the 2nd meal was provided. After finishing the 2nd meal, participants continued 2 min of walking every 20 min for the following 120 min. After data collection was completed in the laboratory, participants were asked to keep wearing the same pedometer and to record any food and liquid consumption for the rest of the day (until midnight) under free-living conditions.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Physical activity level | Changes in physical activity level (steps) in the free-living conditions | 480 minutes | 
| Energy intake | Changes in energy intake (kcal) in the free-living conditions | 480 minutes | 
| Cognitive performance | Changes in cognitive performance (% changes in accuracy) including Task Switching and Flanker tasks | 320 minutes | 
| Cognitive function | Changes in cognitive performance (% changes in reaction time) Task Switching and Flanker tasks | 320 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Postprandial glucose concentrations | Changes in postprandial finger pricking glucose concentrations | 320 minutes | 
| Blood pressure | Changes in systolic blood pressure and diastolic blood pressure | 320 minutes | 
| Visual analogue scale (VAS) | Changes in self-perceived sensations including appetite, energy and mood (score from 0-100) | 320 minutes | 
| Continuous glucose monitoring | Changes in different depots of adipose tissue interstitial glucose concentrations | 320 minutes | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Breaking up sitting, Eating behaviour, Spontaneous physical activity, Executive function, Energy balance