Preclinical Brain Tumor Models

Study Overview
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Brief Summary
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Background: Primary or secondary brain tumors (metastases) remain associated with a very poor prognosis linked to significant therapeutic resistance. Thus, glioblastoma, which is the most common and aggressive primary brain tumor in adults, is associated with inevitable relapses within 7 to 10 months and median survival of approximately 12 to 14 months. At the same time, brain metastases are increasingly increasing following better systemic control of other metastatic sites and improvement in detection methods. However, they remain resistant to the latest therapeutic innovations such as immunotherapies or targeted therapies. In this context, innovative strategies are necessary to identify new therapeutic targets and implement new treatments to overcome resistance phenomena in the clinic. Objective: Our goal will be to generate tumoroids and stem-like cell lines (PDX) from patient tumor samples. Methods: We will establish tumor tumoroids and stem cell lines from patient samples. These preclinical models will allow us to test a large number of drugs, quickly and efficiently thanks to models as close as possible to patient tumors and limiting the use of animal models (3R). Overall, this project should enable major advances in the treatment of glioblastoma and brain metastases and enable the rapid testing of new molecules in clinical trials thanks to the homology of our models with our patients' diseases.

Official Title
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Establishment of Pre-clinical Stem Line and Tumoroid Models of Brain Tumors

Conditions
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Brain Metastases, Diffuse Gliomas

Intervention / Treatment
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* Other: Fresh tumor sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adult patients Brain tumor neuro-imaging Eligible for neurosurgery Exclusion Criteria: unresectable tumor

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| patients with primitive tumor<br>Patient samples will be collected during routine care neurosurgical resection. | Other: Fresh tumor sampling<br>* Patient samples will be collected during neurosurgical resection. .<br>|
| patients with brain metastasis<br>Patient samples will be collected during routine care neurosurgical resection. | Other: Fresh tumor sampling<br>* Patient samples will be collected during neurosurgical resection. .<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Tumoroid and stem cell line generation | The generation of tumoroids and stem cell lines will be attested at 4 weeks after initiation of their culture. Tumoroids: generation of round and viable structures Stem cell lines: generation of neurospheres | 4 weeks after generation | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Brain metastases, Glioma, Glioblastoma, Preclinical models, Tumoroids, Cell lines