The Relation of High Sensitivity C Reactive Protein and Total Leucocytic Count Levels in Threatened Abortion

Study Overview
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Brief Summary
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to identify the relation between maternal serum high sensitivity C reactive protein and total and differential leucocytic count values in healthy pregnant women with threatened abortion and the outcome of pregnancy

Detailed Description
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observational prospective cohort research will be pregnant women attending to obstetrics and gynecology department of University Hospital. After enrollment, participants will be divided into two groups: Study group: had 120 healthy women presenting with threatened miscarriage. Control group: had 120 healthy women with an uncomplicated single pregnancy. informed permission will be obtained. All cases subjected to the following: taking complete history, General examination, Special investigations including Obstetric ultrasonography for assessment of gestational age, and exclusion of congenital anomalies or multi-fetal gestation. Laboratory investigations including Estimation of the complete blood picture (CBC), Highly sensitive CRP (HS-CRP) single measurement, Women over 1st trimester abdominal ultrasonographic examination (6-13weeks) using Toshiba with transabdominal transducer probe frequency of 7 MHz to evaluate the following data: Measuring of the fetal crown-rump length (CRL) done to confirm the fetal gestational age and the viability of the fetus. Gestational age will be calculated from the beginning of the last regular menstrual cycle. Women who had hormone therapy or progesterone supplements, as well as pregnancies with a discrepancy between LMP and crown rump length of more than three days, were excluded from the research. Pregnant women will be under close follow up every 2 weeks by: Obstetric U/S, Measure blood pressure and inform us if severe bleeding occurs.

Official Title
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The Relation Between Maternal Serum High Sensitivity C Reactive Protein and Total and Differential Leucocytic Count Levels in Healthy Pregnant Women With Threatened Abortion and the Outcome of Pregnancy

Conditions
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Abortion Early

Intervention / Treatment
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* Diagnostic Test: maternal serum C reactive protein
* Diagnostic Test: total leucocytic count levels
* Diagnostic Test: differential leucocytic count levels


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Single intrauterine pregnancy with vaginal hemorrhage and/or abdominal pain in the study group, an ultrasound showing an intrauterine gestational sac with a live fetus and a regular fetal heartbeat, with/without a sub-chorionic hematoma, healthy women who come for routine antenatal care with spontaneous pregnancy. Exclusion Criteria: Age over 40 smoking multiple pregnancies endocrinological diseases history of repeated spontaneous abortions.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Study group<br>120 healthy women presenting with threatened miscarriage. | Diagnostic Test: maternal serum C reactive protein<br>* maternal serum high sensitivity C reactive protein<br>Diagnostic Test: total leucocytic count levels<br>* total leucocytic count levels<br>Diagnostic Test: differential leucocytic count levels<br>* differential leucocytic count levels<br>|
| Control group<br>120 healthy women with an uncomplicated single pregnancy. | Diagnostic Test: maternal serum C reactive protein<br>* maternal serum high sensitivity C reactive protein<br>Diagnostic Test: total leucocytic count levels<br>* total leucocytic count levels<br>Diagnostic Test: differential leucocytic count levels<br>* differential leucocytic count levels<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Highly sensitive CRP (HS-CRP) | A high level of hs-CRP, i.e., greater than 3 mg/L, | 9 months | 
| total leucocytic count | measures the number of white blood cells (WBCs) in blood | 9 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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C reactive protein, total and differential leucocytic count, abortion