Effects of Hardaliye, a Fermented Grape Drink, on Oxidative Stress, Lipid Profile and Blood Pressure in Young Footballers

Study Overview
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Brief Summary
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In the present study, the authors aimed to investigate the effects of hardaliye (grape juice) consumption on oxidative stress parameters, lipid profile and blood pressure in young football players.

Official Title
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Effects of Hardaliye, a Fermented Grape Drink, on Oxidative Stress, Lipid Profile and Blood Pressure in Young Footballers: A Randomised Trial

Conditions
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Diet, Healthy, Antioxidative Stress

Intervention / Treatment
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* Other: Grape juice
* Other: Placebo drink


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Being a male football player playing in a team in the Elite Development League, Participating in training with the team at least 5 days a week for 3 months, Not consuming red wine, grape juice, and/or hardaliye regularly before the study was started, Those who stayed in the sports facility where catering service was provided for breakfast, lunch and dinner were included in the study. Exclusion Criteria: With chronic disease, Smoker, Those taking antioxidant supplements, Non-cooperative subjects or unwilling to sign an informed consent and participate in the study.

Ages Eligible for Study
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Minimum Age: 15 Years
Maximum Age: 24 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Hardaliye Group<br>Hardaliye Group consumed 250 ml/day of hardaliye (grape juice) for 28 days. | Other: Grape juice<br>* Hardaliye (grape juice) is a fermented, traditional non-alcoholic beverage made from fresh grapes. It is mainly produced by the fermentation of grapes, sour cherry leaves and mustard seeds. Hardaliye was purchased from traditional producers in Turkey. Hardaliye Group consumed 250 ml/day of hardaliye (grape juice) for 28 days.<br>|
| Placebo Comparator: Placebo Group<br>Placebo Group consumed 250 ml/day of placebo drink for 28 days. | Other: Placebo drink<br>* Placebo drink has carbohydrate, energy and sensory properties similar to hardaliye. Placebo Group consumed 250 ml/day of placebo drink<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in total antioxidant capacity | Measurement of serum level of total antioxidant capacity (μmol Trolox eq/L). | 28 days | 
| Change in total oxidation status | Measurement of serum level of total oxidation status (μmol H2O2 eq/L). | 28 days | 
| Change in oxidative stress index | (Serum level of total oxidation status/serum level of total antioxidant capacity) × 100 | 28 days | 
| Change in lipid parameters | Measurement of serum levels total cholesterol (mg/dl), HDL-cholesterol (mg/dl), LDL-cholesterol (mg/dl) and triglycerides (mg/dl). | 28 days | 
| Change in blood pressure | Measurement of systolic (mm Hg) and diastolic blood pressure (mm Hg) | 28 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in perceived exercise-induced pain | Perceived exercise-induced pain was determined using the 100-mm Visual Analog Scale. 100-mm Visual Analog Scale scores range from 0 (no pain) to 100 (most severe pain). A higher score indicates a higher perceived pain level. | 28 days |