Evaluation of the Antimicrobial Fiber Reinforced Composite Resin Space Maintainer Modified With Silver Nano Particles

Study Overview
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Brief Summary
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Fiber reinforced composite resin space maintainer

Detailed Description
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Evaluation of the antimicrobial fiber reinforced composite resin space maintainer modified by silver nano particles.

Official Title
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Evaluation of the Antimicrobial Fiber Reinforced Composite Resin Space Maintainer Modified With Silver Nano Particles

Conditions
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Dental Caries in Children

Intervention / Treatment
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* Device: Fiber reinforced composite resin space maintainer


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Premature loss of primary molar. Presence of teeth on the mesial and distal sides of the extraction space. Normal primary molar relation. No root resorption of abutment teeth. Presence of a succedaneous tooth bud. Absence of pathology on the eruption track of the succedaneous tooth. The age will be ranged from 4 to 9 years. Exclusion Criteria: Carious buccal and lingual surface of abutment teeth. Absence of teeth on the mesial and distal sides of the extraction.

Ages Eligible for Study
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Minimum Age: 4 Years
Maximum Age: 9 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Fiber reinforced composite space maintainer<br>12 children received fiber reinforced composite (FRC) space maintainer without silver nanoparticle. | Device: Fiber reinforced composite resin space maintainer<br>* Bonding of fiber reinforced with composite resin on primary second molar and primary canine under rubber dam isolation<br>|
| Experimental: Fiber reinforced space maintainer with silver nano particles<br>12 children received fiber reinforced composite (FRC) space maintainers modified with silver nanoparticles. | Device: Fiber reinforced composite resin space maintainer<br>* Bonding of fiber reinforced with composite resin on primary second molar and primary canine under rubber dam isolation<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Evaluation of strepetoccous mutant and lactobacilli from sample of saliva | Streptococcus mutants : One loop (1/1000th ml of sample) was inoculated on the Mitis Salivarius (MSA)* agar with 0.2 U/ml bacitracin and sucrose (15% w/v) bacitracin. The plates were incubated aerobically ( 5% CO2) at 37°C for 48 h.(10) For determination of S. mutans count: After 48 h , colony characteristics were studied and the number of colony forming units of SM (CFU/ml) in saliva was determined manually . Lactobacilli : One loop (1/1000th ml of sample) was inoculated on the Rogosa **SL agar plate. The plates were incubated aerobically ( 5% CO2) at 37°C for 48 h.(11) For determination of lactobacilli count: After 48 h , colony characteristics were studied and the number of colony forming units of lactobacilli (CFU/ml) in saliva was determined manually | 6 month |