Effect of Hebal and Synthetic Medicamentss on Enterococcus Feacalis

Study Overview
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Brief Summary
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this study aims to evaluate the antimicrobial efficacy of two synthetic (Ibuprofen, Modified triple antibiotic paste) and two herbal materials (Curcumin and Olive leaves extract) used as intracanal medication, against E.Faecalis in secondary endodontic infection cases.

Detailed Description
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we evaluate the antimicrobial efficacy of two synthetic (Ibuprofen, Modified triple antibiotic paste) and two herbal materials (Curcumin and Olive leaves extract) used as intracanal medication, against E.Faecalis in secondary endodontic infection cases by taking three samples from root canal , the first sample after removing gutta percha from root canal system, the second sample after shaping with protaper universal files and cleaning with 2.5% sodium hypochlorite , the third sample after 7 days from application of the medication . samples will be diluted into ten fold serial dilution and then the diluted samples will be cultured on bile esculin agar as it is selective media for enterococcus feacalis. bacterial reduction will be determined and comparision will be made .

Official Title
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Antimicrobial Efficacy of Synthetic Versus Herbal Intracanal Medicaments Against Enterococcus Faecalis

Conditions
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Effective Intracanal Medicaments Against Enterococcus Faecalis

Intervention / Treatment
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* Drug: Ibuprofen 400 mg.
* Drug: modified triple antibiotic paste
* Dietary Supplement: curcumin
* Dietary Supplement: olive leaves extract


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Single rooted teeth. Teeth have clinical signs and symptoms of endodontic failure as sensitivity to percussion, pain, swelling or fistula. Teeth with radiographic features of endodontic failure as persistent periapical lesion or widening of periodontal ligament. Exclusion Criteria: • Teeth with fractures of the crown or root. Teeth with periodontal pocket deeper than 4mm. Teeth have procedural error like ledge, broken instrument, or perforation.
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: group 1<br>ibuprofen as intracanal medicament | Drug: Ibuprofen 400 mg.<br>* synthetic intracanal medicament<br>|
| Active Comparator: group 2<br>modified triple antibiotic paste as intracanal medicament | Drug: modified triple antibiotic paste<br>* synthetic intracanal medicament<br>* Other names: clindamycin, ciprofloxacin and metronidazole;|
| Active Comparator: group 3<br>curcumin as intracanal medicament | Dietary Supplement: curcumin<br>* herbal intracanal medicament<br>|
| Active Comparator: group 4<br>olive leaves as intracanal medicament | Dietary Supplement: olive leaves extract<br>* herbal intracanal medicament<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| measurment of microbial reduction as result of applying synthetic and herbaal intracanal medicaments | sample 1 will be taken after gutta percha removal sample 2 will be taken after cleaning and shaping sample 3 will be taken after apllying the intracanal medicament for one week | time necessary for incubation of each sample is 24 hour |