Effects of a High-fat Meal on Exercise-mediated Sympatholysis

Study Overview
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Brief Summary
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The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are: What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal Can dietary nitrate prevent the declines in exercise-mediated sympatholysis Participants will be asked to complete three study visits in a randomized order: Low-fat meal High-fat meal High-fat meal plus dietary nitrate Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.

Official Title
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Effects of a High-fat Meal on Exercise-mediated Sympatholysis

Conditions
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Diet, Healthy

Intervention / Treatment
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* Dietary Supplement: Beetroot drink
* Dietary Supplement: Placebo
* Dietary Supplement: Control


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18-30 years of age Healthy, free of known cardiovascular, metabolic, or musculoskeletal disease Exclusion Criteria: Diagnosed cardiovascular or metabolic disease, injury preventing handgrip exercise, prescription of chronic medications other than oral contraceptives. Inability to abstain from drugs (except oral contraceptives), caffeine, alcohol, strenuous physical activity, mouthwash, and multivitamins prior to study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: High-fat meal with Beetroot Drink<br>2 sausage egg McMuffins and a hashbrown from McDonalds and 10g of beetroot powder mixed in 250 mL cold tap water drink | Dietary Supplement: Beetroot drink<br>* Drink containing 10g of beetroot powder in 250 mL cold tap water<br>* Other names: Superbeets;|
| Placebo Comparator: High-fat meal with Placebo Drink<br>2 sausage egg McMuffins and a hashbrown from McDonalds and Mio water flavouring in 250 mL cold tap water. Trace amounts of sugar and sodium were mixed to match the beetroot supplement | Dietary Supplement: Placebo<br>* Mio flavouring in 250 mL cold tap water. Trace amounts of sugar and sodium were added to match the content in the beetroot supplement<br>* Other names: Mio;|
| Sham Comparator: Low-fat meal<br>Kelloggs cornflakes (110g), greek yogurt (400g), skim milk (500 mL), and orange juice (250 mL). Energy-matched to the high-fat meal | Dietary Supplement: Control<br>* Low-fat meal with cornflakes, greek yogurt, skim milk, and orange juice<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Forearm vascular conductance | Duplex ultrasound of brachial artery | Before intervention and 1, 2, 3 hours post-intervention | 
| Forearm blood flow | Duplex ultrasound of brachial artery | Before intervention and 1, 2, 3 hours post-intervention | 
| Blood Pressure | finger plethysmography | Before intervention and 1, 2, 3 hours post-intervention | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Norepinephrine | ELISA kit | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date | 
| Nitrate/Nitrite concentration | ELISA kit | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date | 
| Blood Glucose | Blood glucose monitor | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood analyzed immediately after collection at each time point | 
| Triglycerides | Triglyceride assay kit | Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Functional sympatholysis, Exercise