Inflammatory Stress and Periodontal Tissue Destruction

Study Overview
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Brief Summary
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Metabolic syndrome and periodontitis are diseases that lower the quality of life and their incidence rates are increasing. Since both of these diseases are associated with systemic inflammation and insulin resistance, they may be comorbid. The aim of this study was to evaluate the effect of interleukin-20 (IL-20) on periodontal destruction in individuals with metabolic syndrome while considering the relationship between metabolic syndrome and periodontitis. This study included a total of 80 individuals who were systemically and periodontally healthy (CG, n=20), were systemically healthy and had periodontitis (PG, n=20), had metabolic syndrome and were periodontally healthy (MG, n=20), or had both metabolic syndrome and periodontitis (MPG, n=20). Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses.

Official Title
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Inflammatory Stress and Periodontal Tissue Destruction in Patients With Metabolic Syndrome and Periodontitis

Conditions
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Oxidative Stress, Inflammation

Intervention / Treatment
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* Other: Gingival crevicular fluid (GCF) and serum samples


Participation Criteria
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Eligibility Criteria
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Study groups were created based on the guidelines of the 2017 Classification of Periodontal Diseases and the 2005 Metabolic Syndrome Diagnostic Criteria of the Endocrinology and Metabolic Society of Turkey Inclusion Criteria: all participants were required to have at least 20 teeth and be between the ages of 20 and 65 years Exclusion Criteria: Smokers, patients who had used antibiotics or anti-inflammatory and antioxidant drugs in the last 6 months, patients who were pregnant or lactating, those who had cancer or autoimmune diseases, and those who had undergone any periodontal treatment in the last 6 months

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| systemically and periodontally healthy (CG)<br>Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses. | Other: Gingival crevicular fluid (GCF) and serum samples<br>* Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses<br>|
| systemically healthy and periodontitis (PG)<br>Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses | Other: Gingival crevicular fluid (GCF) and serum samples<br>* Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses<br>|
| Metabolic syndrome and periodontally healthy (MG)<br>Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses | Other: Gingival crevicular fluid (GCF) and serum samples<br>* Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses<br>|
| Metabolic syndrome and periodontitis (MPG)<br>Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses | Other: Gingival crevicular fluid (GCF) and serum samples<br>* Gingival crevicular fluid (GCF) and serum samples were collected from all participants for biochemical analyses<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| concentration of gingival crevicular fluid (GCF) parameters | interleukin-20, RANKL, OPG values evaluated in GCF. | 1 year | 
| concentration of serum parameters | interleukin-20, RANKL, OPG, MMP-8, TOS, TAS values evaluated in serum | 1 year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| value of periodontal clinical parameters | plaque index, gingival index, bleeding on probing, Clinical attachment loss, probing pocket depth | 1 year | 
| value of patients' clinical metabolic syndrome parameters | Body mass index (BMI), abdominal circumference, and HbA1c | 1 year | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Metabolic syndrome, periodontitis, interleukin-20