Effect of Gene Polymorphism on Cognitive Function

Study Overview
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Brief Summary
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The goal of this observational study is to study the effect of gene polymorphism on cognitive improvement in response to exercise in healthy participants. The main questions it aims to answer are: To investigate whether FNDC5/Irisin genotype polymorphism affects cognitive function and inter-individual variability in exercise response. To explore whether the FNDC5/Irisin gene polymorphism can act alone or interact with the BDNF gene to cause inter-individual differences in cognitive function responses to exercise gain. Participants will be asked to perform high-intensity interval training( HIIT) on a cycle ergometer. Each high-intensity training period will be 30 seconds followed by a 4-minute rest period. The participants will also perform cognitive tests (Vistorian stroop test and Fitlight trainer test) before and after the exercise.

Detailed Description
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Exercise has a significant effect on improving brain function. It can improve cognitive function, enhance learning effects, and prevent the occurrence of neurodegenerative diseases in the elderly. Exercise's mechanism of action to improve cognitive function includes stimulating the nervous system to secrete brain-derived neurotrophic factor (BDNF) and skeletal muscle to secrete irisin. However, the effect of exercise on improving physical function varies from person to person. This phenomenon is called inter-individual exercise response variability. Genetic differences may be responsible for this phenomenon. The genotype differences of BDNF and Irisin can cause cognitive function. However, the scientific community is still unclear whether the differences between the two genotypes are related to individual differences in exercise responses. The investigators suspect that genotype might be responsible for interindividual variability in motor responses and therefore designed this experiment to test this thesis. This study is expected to recruit 200 healthy adults to complete the genotype sequencing of BDNF and Irisin and use the cognitive function responses before and after a single high-intensity interval aerobic exercise to establish the model that genotype affects the variability of individual exercise responses. The experimental results of this study can help the scientific community understand the influence of genotype on individual differences in exercise response and further explore and refine the design of exercise prescription.

Official Title
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The Effect of Irisin Polymorphism on Cognitive Function and Inter-individual Exercise Response Variability

Conditions
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Polymorphosis, Cognitive Function

Intervention / Treatment
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* Other: High intensitiy interval training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Taiwanese National who perform less than 120 minutes of aerobic exercise per week. The subjects will refrain from drinking alcoholic or caffeinated drinks 24 hours prior to the participation in the study Exclusion Criteria: Subjects who are diagnosed with Hypertension Arrhythmia Depression Color blindness Musculoskeletal injury within one year Body Mass index higher than 25 Pregnant females

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: High intensitiy interval training|The participants will perform HIIT at 85% of their heart rate reserve. There will be 8 cycles of high intensity with four minutes of rest intervals in between.|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in Serum BDNF levels | Serum brain derived neurotrophic factor levels | The blood sample will be collected immediately after the participant has signed the consent to assess pre exercise BDNF levels | 
| Change in Serum BDNF levels | Serum brain derived neurotrophic factor levels | The blood sample will be collected 30 minutes after the participant has completed high intensity exercise to assess post exercise BDNF levels | 
| Change in Serum Irisin Levels | Serum Irisin levels | The blood sample will be collected immediately after the participant has signed the consent to assess pre exercise Irisin levels | 
| Change in Serum Irisin Levels | Serum Irisin levels | The blood sample will be collected 30 minutes after the participant has completed high intensity exercise to assess post exercise Irisin levels | 
| Change in Stroop Reaction time | Reaction time for Victorian version of Stroop test | Baseline | 
| Change in Stroop Reaction time | Reaction time for Victorian version of Stroop test | Immediately after the procedure | 
| Change in Fitlight reaction time | Mean Reaction time to Fitlight Trainer complex patterns | Baseline | 
| Change in Fitlight reaction time | Mean Reaction time to Fitlight Trainer complex patterns | Immediately after the procedure | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Monitor the Heart rate | Effect of gene polymorphism on changes in heart rate variability due to exercise. | During the procedure | 
| Change in Fitlight simple reaction time | Reaction time to Fitlight | Baseline | 
| Change in Fitlight simple reaction time | Reaction time to Fitlight | Immediately after the procedure | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Irisin, Cognition, Polymorphism, High intensity interval training