Microbiome Sampling in GI Disease With a Focus on Small Intestinal Microbial Assessment

Study Overview
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Brief Summary
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GI disorders are influenced by the gut microbiome. To date, sampling of the small intestine in GI disorders has been limited. The investigators plan to sample the small intestinal contents during endoscopy for research purposes.

Detailed Description
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Current guidelines (AGA Clinical Practice Update, 2020) report the definition of SIBO as a clinical entity lacks precision and consistency; it is a term generally applied to a clinical disorder where symptoms, clinical signs, and/or laboratory abnormalities are attributed to changes in the numbers of bacteria or in the composition of the bacterial population in the small intestine. To date, there is unlimited knowledge regarding the diagnostic criterion which has been limited by nonspecific and nonsensitive testing such as breath tests. Breath tests have a limited use in patients with IBS-D who inherently have increased gut transit time rendering the testing invalid for accurately measuring small intestinal bacteria. Additionally, the relationship between SIBO and symptoms in patients without obvious risk factors (such as anatomical changes due to surgery) is unknown. The investigators study aims to investigate the microbial landscape of the small intestine in healthy patients and those with GI disease (suspected or diagnosed) undergoing an upper endoscopy by collecting an aspirate of patient small intestinal fluid and studying it.

Official Title
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Microbiome Sampling in GI Disease With a Focus on Small Intestinal Microbial Assessment

Conditions
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Healthy, IBS - Irritable Bowel Syndrome, Inflammatory Bowel Diseases

Intervention / Treatment
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* Other: endoscopic sampling of luminal fluid


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age >18years Patients seen at Stanford University Digestive Health Center who are scheduled for an upper endoscopy as part of their Standard Of Care Exclusion Criteria: Children (under age 18years) Pregnant Women and Fetuses Neonates (0 - 28 days) Impaired Decision Making Capacity

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 100 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: endoscopic sampling of luminal fluid|We will collect luminal fluid via endoscopic sampling|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Ability to isolate live bacteria from 95% of samples | We aim to isolate live bacteria from 95% of samples | 1-4 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Ability to measure quantitative Colony Forming Units (CFUs) in 90% of samples | We aim to measure quantitative Colony Forming Units (CFUs) in 90% of samples | 1-4 years |