Effects of Dietary Nitrate Intake on Cardiovascular Performance in Response to Hand Grip Test in Bodybuilders

Study Overview
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Brief Summary
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Through a randomized, crossover, double-blind, placebo-controlled clinical trial, 20 bodybuilders will participate a acute-day trial with two intervention protocols: 1) placebo and 2) nitrate; in which will ingest beet juice with or without NO3 in its composition with a 7-day washout interval.

Official Title
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Effects of Dietary Nitrate Intake on Cardiovascular Performance in Response to Hand Grip Test in Bodybuilders

Conditions
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Steroid Abuse, Cardiovascular Abnormalities

Intervention / Treatment
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* Dietary Supplement: Beetroot juice rich in nitrate
* Dietary Supplement: Beetroot juice depleted in nitrate


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Anabolic steroid use in the past or actually; Participation at least one bodybuilding competition; Exclusion Criteria: Previous history of acute myocardial infarction and/or stroke; Allergy or intolerance to nitrate, gluten, or milk; Medications (proton pump inhibitors, beta-blockers, calcium channels antagonists).

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Experimental (Beetroot juice NO3)<br>Acute ingestion of beetroot juice rich in NO3 (800mg). | Dietary Supplement: Beetroot juice rich in nitrate<br>* The participant will intake beetroot juice rich in NO3 (800mg). Two hours after intake the last bottle of juice, the participants will go to the laboratory for the handgrip and cardiovascular tests evaluation. Before crossover protocol a 7-day washout interval will be given for the purification of the compounds provided by the protocols.<br>|
| Placebo Comparator: Placebo (Beetroot juice without NO3)<br>Acute ingestion of beetroot juice rich depleted in NO3. | Dietary Supplement: Beetroot juice depleted in nitrate<br>* The participant will intake beetroot juice depleted in NO3. Two hours after intake the last bottle of juice, the participants will go to the laboratory for the handgrip and cardiovascular tests evaluation. Before crossover protocol a 7-day washout interval will be given for the purification of the compounds provided by the protocols.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Macrovascular function (Flow mediated dilatation%) |  | up to 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasmatic concentrations of nitrate and nitrite (mmol/L) |  | up to 2 years | 
| Values of systolic and diastolic blood pressure (mmHg) |  | up to 2 years | 
| Values of heart rate variability (ms) |  | up to 2 years | 
| Values of heart rate (beats per minute) |  | up to 2 years | 
| Values of hand grip strength (kgF) |  | up to 2 years |