Effects of Hydrogen Gas Inhalation on Adults of Various Ages

Study Overview
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Brief Summary
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Herein, investigated the effects of 4 weeks of H2 gas inhalation on community-dwelling adults of various ages.

Detailed Description
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Molecular hydrogen (H2) is a versatile therapeutic agent. H2 gas inhalation is reportedly safe and has a positive impact on a range of illnesses, including Alzheimer's disease (AD). Herein, investigated the effects of 4 weeks of H2 gas inhalation on community-dwelling adults of various ages. Fifty-four participants including those dropped out (5%) were screened and enrolled. The selected participants were treated as a single group without randomization. Evaluated the association between total and differential white blood cell (WBC) counts and AD risk at individual levels after 4 weeks of H2 gas inhalation treatment. Furthermore, evaluation of dementia-related biomarkers such as beta-site APP cleaving enzyme 1 (BACE-1), amyloid beta (Aβ), brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor A (VEGF-A), T-tau, monocyte chemotactic protein-1 (MCP-1), and inflammatory cytokines (interleukin-6).

Official Title
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Effects of Hydrogen Gas Inhalation on Community-Dwelling Adults of Various Ages: A Single-Arm, Open-Label, Prospective Clinical Trial

Conditions
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Alzheimer Disease

Intervention / Treatment
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* Other: H2 gas inhalation


Participation Criteria
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Eligibility Criteria
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Inclusion Age ranges 40-70 years Healthy participants Patients who are willing or able to follow the doctor's instructions Fully understand the purpose and procedure of this clinical trial Exclusion Criteria Participants excluded those who have any disease Pregnant and lactating women

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Intervention Model: Single Group Assignment
Interventional Model Description: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Single arm<br>Single group treatment | Other: H2 gas inhalation<br>* H2 gas inhalation treatment for 4 weeks in on community dwelling adults of various ages<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurement of white blood cell and its differential counts such as neutrophils (%), basophils (%) | 4 weeks treatment of H2 gas inhalation in community dwelling adults of various ages | 4 weeks | 
| Measurement of oxidative stress markers such as reactive oxygen species (µM), and nitric oxide (µM) | 4 weeks treatment of H2 gas inhalation in community dwelling adults of various ages | 4 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurement of dementia biomarkers such as brain-derived neurotrophic factor (BDNF ng/mL) | 4 weeks treatment of H2 gas treatment | 4 weeks | 
| Measurement of dementia biomarkers such as brain-derived neurotrophic factor amyloid beta (Aβ (pg/mL)) | 4 weeks treatment of H2 gas treatment | 4 weeks | 
| Measurement of inflammatory markers such as interleukin 6 (pg/mL) | 4 weeks treatment of H2 gas treatment | 4 weeks |