Developmental Endothelial Locus-1, Platelet Count, and Soluble P-selectin in Overweight and Obese Subjects

Study Overview
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Brief Summary
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This observational study aims to detect serum levels of Developmental Endothelial Locus-1 (DEL-1), Platelet Count, and Soluble P-selectin (sP-sel) in Overweight and Obese Subjects. The main questions it aims to answer are: Is there a difference in serum DEl-1 levels in overweight and obese subjects compared to normal subjects? Are there any relations between serum DEL-1 levels, sP-sel, and platelet count in overweight and obese subjects ?

Official Title
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Serum Levels of Developmental Endothelial Locus-1 and Its Relationship With Platelet Count and Soluble P-selectin in Overweight and Obese Subjects

Conditions
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Overweight and Obesity

Intervention / Treatment
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* Diagnostic Test: Platelet count
* Diagnostic Test: Developmental endothelial locus-1 (DEL-1)
* Diagnostic Test: soluble P-selectin (sP-sel)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male patients. Age (18-65) years. Healthy overweight and subjects. Exclusion Criteria: Patients with cardiovascular diseases. Diabetic patients. Previous history of thrombo-embolic diseases. Patients with hyperthyroidism. Patients with any type of malignancy. Underlying hepatic or renal failure. Major surgery within 60 days.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Control<br>22 healthy age-matched normal-weight male subjects (BMI is from 18.5 to < 25) | Diagnostic Test: Platelet count<br>* Platelet count in study groups<br>Diagnostic Test: Developmental endothelial locus-1 (DEL-1)<br>* Serum DEL-1 in study groups<br>Diagnostic Test: soluble P-selectin (sP-sel)<br>* Serum sP-sel in study groups<br>|
| Overweight<br>22 healthy overweight male subjects (BMI is from 25 to < 30). Patients with cardiovascular diseases, any malignancy, hepatic or renal failure, diabetic, hyperthyroid patients, patients with a previous history of thromboembolic diseases, or with a history of major surgery within 60 days will be excluded. | Diagnostic Test: Platelet count<br>* Platelet count in study groups<br>Diagnostic Test: Developmental endothelial locus-1 (DEL-1)<br>* Serum DEL-1 in study groups<br>Diagnostic Test: soluble P-selectin (sP-sel)<br>* Serum sP-sel in study groups<br>|
| Obesity<br>22 healthy obese male subjects (BMI is ≥ 30). Patients with cardiovascular diseases, any malignancy, hepatic or renal failure, diabetic, hyperthyroid patients, patients with a previous history of thromboembolic diseases, or with a history of major surgery within 60 days will be excluded. | Diagnostic Test: Platelet count<br>* Platelet count in study groups<br>Diagnostic Test: Developmental endothelial locus-1 (DEL-1)<br>* Serum DEL-1 in study groups<br>Diagnostic Test: soluble P-selectin (sP-sel)<br>* Serum sP-sel in study groups<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Platelet count | platelet count in venous blood samples in normal-weight, overweight, and obese subjects | 6 months | 
| Serum Developmental endothelial locus-1 levels | Serum developmental endothelial locus-1 in normal-weight, overweight, and obese subjects | 6 months | 
| Serum Soluble P-selectin levels | Serum Soluble P-selectin in normal-weight, overweight, and obese subjects | 6 months |