Changes in the Hormonal and Inflammatory Profile of Young Sprint- and Endurance-trained Athletes.

Study Overview
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Brief Summary
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One essential element of athlete training is conditioning camps, where athletes undergo a rigorous and targeted training schedule to prepare for upcoming sporting events. During sports camps, due to the accumulation of a large number of training units, including high-intensity exercises, athletes react with post-exercise overload, acute fatigue, and overreaching which can become a chronic overtraining syndrome. Overtraining syndrome is a very specific and severe condition where overtraining without adequate rest and recovery leads to performance decrements lasting more than 2-3 months, coupled with a mood disturbance. The exact etiology and pathogenesis are unknown and actively being investigated. During training camps the balance between training volumes and recovery is often a delicate one and, the accumulation of exercise-induced stress may exceed the capacity of both neuroendocrine and immune adaptation leading to an alteration of physiological functions, decreasing adaptation to performance, impairment of psychological processing, immunological dysfunction, and biochemical abnormalities. Moreover, there is currently a lack of biomarkers accessible to assist in diagnosing and, what's even more important - help to prevent the overtraining syndrome, except for the continued presence of unexplained underperformance despite athletes' adequate rest and recovery. Thus, this study aims to explain how long and intensive training for endurance affects the hormonal and immune systems of young athletes. The main questions it aims to answer are: How does intense training influence hormonal and immune responses in young, trained athletes? Does training specialization affect the hormonal and immune response to intense training?

Detailed Description
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Twenty-four young male and female athletes, specializing in athletics disciplines such as sprint (hurdles, 100m, 110m), and endurance: race walk, 5000m, and 10000m, volunteered to take part in this study. Investigation was held during 9 days of the training camp (preparatory period, general preparation sub-period), which was aimed at increasing the endurance and flexibility of athletes. During the training camp, all subjects occupied the same accommodations and followed the same training plan and diet schedules. The camp regime included 2 training sessions per day, lasting 4 hours per day, and a total of 21 hours per 9 days. Blood samples were taken always in the same conditions, from the antecubital veins. Participants were seated in a recumbent position for a minimum of 10 minutes before blood draws to stabilize the hydrostatic condition. Before collection of the first sample (rested baseline), participants were asked to avoid any intense exercise at least 24 hours before sampling. Next samples were taken after 4 days and after another 5 days of training camp (in total after 9 days). The concentration of catecholamines: epinephrine and norepinephrine in serum was measured as well and the responsiveness of the adrenal medulla to the sympathetic nervous activity was estimated by the ratio E/NE calculated. Also, concentrations of cortisol, hs-CRP, and myoglobin were measured and changes in blood morphology were estimated.

Official Title
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Changes in the Hormonal and Inflammatory Profile of Young Sprint- and Endurance-trained Athletes Following a Sports Camp

Conditions
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Overtraining Syndrome

Intervention / Treatment
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* Other: Exercise training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age 15-17 years, minimum of 3 years of training experience, specialization in anaerobic and aerobic disciplines. Exclusion Criteria: presence of acute or chronic inflammation fever, infections, injuries se of any anti-inflammatory drugs.

Ages Eligible for Study
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Minimum Age: 15 Years
Maximum Age: 17 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| sprint-trained<br>Sprint-trained athletes: 6 male and 6 female, aged average 15 y., | Other: Exercise training<br>* Intense endurance training<br>|
| endurance-trained<br>Endurance-trained athletes: 6 male and 6 female, aged average 15 y., | Other: Exercise training<br>* Intense endurance training<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in epinephrine serum level. | marker of HPA axis functioning, stress hormone. | at rest baseline (before training), after 4 - and after another 5 days of training. | 
| Changes in norepinephrine serum level. | marker of HPA axis functioning, stress hormone. | at rested baseline (before training), after 4 - and after another 5 days of training. | 
| Changes in cortisol serum level. | marker of HPA axis functioning, stress hormone | at rested baseline (before training), after 4 - and after another 5 days of training. | 
| Changes in hs-CRP serum level. | marker of inflammatory response. | at rested baseline (before training), after 4 - and after another 5 days of training. | 
| Changes in Myoglobin serum level. | marker of muscle damage | at rested baseline (before training), after 4 - and after another 5 days of training. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in blood morphology. | Morphometric measurements of blood cell. | sat rested baseline (before training), after 4 - and after another 5 days of training. | 
| Changes in epinephrine/norepinephrine ratio. | marker of responsiveness of the adrenal medulla to sympathetic nervous activity. | at rested baseline (before training), after 4 - and after another 5 days of training. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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epinephrine, norepinephrine, cortisol, athletes, fatigue