The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling

Study Overview
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Brief Summary
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The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a [2H5]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.

Official Title
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The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling

Conditions
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Interventional

Intervention / Treatment
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* Dietary Supplement: Collagen and Vitamin C
* Dietary Supplement: Carbohydrate Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy, recreationally active, male and female participants. Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire. Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study. Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial. Participants will be aged 18-35 years old. Participants must be normal to overweight (e.g., BMI 18.5-30). Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial). Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants). Exclusion Criteria: Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor. Self-reported regular tobacco use. Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.). Regular use of anti-inflammatory drugs (e.g., ibuprofen). Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount). Individuals who have participated in studies within the past year involving any of the stable isotopes in the study. Use of birth control and discontinued use in the last 3-months (female only).

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Crossover to receive Collagen and Vitamin C (CVC) or Carbohydrate placebo. Unilateral leg exercise (EX) to give 4 outcomes. (1) CVC-EX; (2) CVC-REST; (3) PLACEBO-EX; (4) PLACEBO-REST
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Collagen and Vitamin C<br> | Dietary Supplement: Collagen and Vitamin C<br>* Collagen-modelled crystalline amino acids (30g total, 8g glycine) and vitamin C (500 mg).<br>* Other names: CVC;|
| Placebo Comparator: Carbohydrate Placebo<br> | Dietary Supplement: Carbohydrate Placebo<br>* Iso-caloric Placebo (30g maltodextrin).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Enrichment of Muscle Collagen | Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into mature collagen protein isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS. | 9 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Area of muscle with localized collagen remodelling | Immunofluorescence determined localization of collagen breakdown and synthesis using collagen hybridizing peptide and procollagen antibodies, respectively, on human skeletal muscle cross-sections. | 9 hours | 
| Concentration of Urinary Growth Hormone | Urine will be collected overnight to measure growth hormone secretion using chemiluminescence. | 8 hours | 
| Enrichment of Myofibrillar Protein | Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into myofibrillar proteins isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS. | 9 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Collagen, Vitamin C, Carbohydrate placebo