Investıgatıon of The Effıciency of Pulsed Electromagnetıc Field Therapy and Stretching Exercises

Study Overview
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Brief Summary
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In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.

Detailed Description
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Purpose: The aim of this study was to evaluate the efficacy of stretching exercise and the pulsed electromagnetic field (PEMF) method in the treatment of muscle injury (INJ). Methods: In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.

Official Title
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Investigation of The Efficiency of Pulsed Electromagnetic Field Treatment and Stretching Exercise In Experimental Skeletal Muscle Injury Model

Conditions
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Muscle Injury, Pulsed Electromagnetic Therapy, Muscle Stretching Exercise

Intervention / Treatment
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* Other: Exercise
* Device: Pulsed Electromagnetic Field Therapy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy young wistar albino rats Exclusion Criteria: not having any injury

Ages Eligible for Study
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Minimum Age: 10 Months
Maximum Age: 11 Months

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: Control<br> | |
| Experimental: Stretching Exercise<br> | Device: Pulsed Electromagnetic Field Therapy<br>* 5 day/week, two week,two sessions consisting of 180 minutes<br>|
| Experimental: Pulsed Electromagnetic Field<br> | Other: Exercise<br>* 5 day/week, two week, one session stretching exercises consisting of ten repetitions of one minute<br>|
| Experimental: Pulsed Electromagnetic Field and Stretching Exercise<br> | Other: Exercise<br>* 5 day/week, two week, one session stretching exercises consisting of ten repetitions of one minute<br>Device: Pulsed Electromagnetic Field Therapy<br>* 5 day/week, two week,two sessions consisting of 180 minutes<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Mononuclear cell infiltration, Atrophy and necrosis, Pyknotic nucleus assesment at the end of two weeks | hematoxylin-eosin staining | two week | 
| VEGF and FGF assesment at the end of two weeks | immunohischemical staining | two week | 
| TGF-β1, eNOS assesment at the end of two weeks | Quantitative Reverse-Transcriptase Polymerase Chain Reaction (RT-qPCR) | two week | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Muscle Injury, TGF-Beta 1, Fibrosis