Effect of Physical Activity Pattern on Cardiometabolic Health

Study Overview
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Brief Summary
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The goal of this study is to understand the interaction between the circadian system and physical activity. Participants will: complete 2 inpatient stays perform moderate exercise be provided with identical meals have frequent blood draws provide urine and saliva samples

Detailed Description
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The endogenous circadian system (i.e., an internal biological rhythm) plays an important role in regulating blood glucose, blood pressure, and energy expenditure. Physical activity improves health and reduces the risk for cardiovascular diseases and type 2 diabetes. The goal of this study is to understand the interaction between the circadian system and physical activity. We aim to test whether such relationship between the circadian system and physical activity can be used to enhance the health benefits of physical activity.

Official Title
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Effect of Physical Activity Pattern on Cardiometabolic Health

Conditions
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Life Style

Intervention / Treatment
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* Behavioral: Exercise intervention


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 20-45 yr old Body mass index (BMI) 20-34.9 kg/m2 No acute, chronic or debilitating medical conditions (e.g. metabolic, cardiovascular, respiratory, neurological, cancers, etc.) Willing to adhere to the protocol requirements for the duration of the study Exclusion Criteria: Currently smoking/vaping or 5 or more years of smoking/vaping Currently pregnant or breastfeeding History of drug or alcohol dependency History of psychiatric illness or disorder Any hospitalization due to COVID-19 Inability to exercise for any reason Any known contraindication to exercise testing based on current ACSM guidelines

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Exercise A-B Intervention<br>The Exercise A first, then the Exercise B intervention. | Behavioral: Exercise intervention<br>* Research participants will be assigned to two exercise conditions.<br>|
| Experimental: Exercise B-A Intervention<br>The Exercise B first, then the Exercise A intervention. | Behavioral: Exercise intervention<br>* Research participants will be assigned to two exercise conditions.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| glucose level | mean glucose level after exercise session | 24-hour test period after exercise | 
| mean arterial pressure | mean arterial pressure after exercise session | 24-hour test period after exercise | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| insulin sensitivity index | Oral Minimal Model method estimates mean insulin sensitivity based on blood glucose and insulin levels from two mixed meal tests after exercise session | about 12 and 24 hours after exercise | 
| beta-cell function index | Oral Minimal Model method estimates mean beta-cell function based on blood glucose, insulin and c-peptide levels from two mixed meal tests after exercise session | about 12 and 24 hours after exercise | 
| total peripheral resistance | total peripheral resistance derived from BP waveform measured by finger plethysmography | 24-hour test period after exercise | 
| autonomic nervous system activity | derived by time-domain heart rate variability analysis | 24-hour test period after exercise | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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exercise, circadian system, physical activity, glucose tolerance, blood pressure