Sweat Response, Sweat Composition and Thermoregulatory Response to Exercise in Heat in Adults With Cystic Fibrosis on Modulator Therapy.

Study Overview
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Brief Summary
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Cystic fibrosis (CF) is a common inherited condition in the Caucasian population resulting in poor function and/or production of the CF transmembrane conductance regulator (CFTR) protein. The CFTR protein plays a crucial role in the secretion and re-absorption of sodium chloride within the sweat gland. The sweat gland has played a key role in diagnosing and understanding CF with sweat chloride elevation being a key criterion to diagnosing CF. People with CF are thought to be at risk of exertional heat illness during exposure to hot environments or during prolonged periods of exercise and are currently encouraged to take salt supplements during periods of excessive sweating. Kaftrio®, a newly approved pharmacological therapy has shown a rapid and sustained reduction in sweat chloride levels on initiation of this treatment. This study will aim to play a crucial part in understanding the sweat response, sweat composition and the thermoregulatory response to exercise in the heat in people with CF on Kaftrio®.

Detailed Description
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Cystic fibrosis (CF) is a common inherited condition in the Caucasian population, affecting approximately 52, 246 people in Europe. Mutations in the CF transmembrane conductance regulator (CFTR) gene results in poor function or production of the CFTR protein which functions as an anion channel to transport ions across the cell membrane. The sweat gland has played a substantial part in diagnosing and understanding CF with sweat chloride elevation being a key criterion to diagnosing CF. Traditionally, abnormal function of the CFTR protein in the sweat gland results in failure to reabsorb sodium chloride leading to excessive salt loss. People with CF are thought to be at risk of exertional heat illness during exposure to hot environments or during prolonged periods of exercise and are currently encouraged to take salt supplements during periods of excessive sweating. Over the last decade pharmacological therapies (CFTR modulator therapies (CFTRm)) which target the underlying cellular defect characterising CFTR in people with CF have been developed. Kaftrio® is now the most widely used modulator therapy. Studies have shown a rapid and sustained reduction in sweat chloride levels on initiation of these highly effective CFTRm. The need for salt supplementation may be influenced when taking CFTRm. Therefore, there is a need to further understand the sweat response relative to the needs in people with CF. This study will recruit 9 people with CF who currently taking Kaftrio® and 9 healthy age-, sex- and weight-matched controls who are ≥ 18 years old. We will ask all participants to attend the laboratories for 2 visits. Visit 1 is designed to calculate metabolic heat production during different cycling intensities in order to set the workload for visit 2 relative to their metabolic heat production. Visit 2 is designed to collect sweat and thermoregulatory indices during 1 hour of cycling in the heat. This study will compare the sweating and thermoregulatory response to exercising in the heat in a group of people with CF who are stable on Kaftrio® compared to a healthy matched control group. The aims of this study are to: Determine the sweat sodium concentration in adults with CF on Kaftrio® from exercise at a fixed-rate of metabolic heat production under heat stress conditions and whether this is different to healthy age- weight- and sex- matched controls; Determine the local and whole-body sweat rate in adults with CF on Kaftrio® during exercise under heat stress conditions and whether this is different to healthy age- weight- and sex- matched controls; Determine how adults with CF on Kaftrio® respond in terms of skin blood flow during exercise under heat stress conditions and whether this is different to healthy age- weight- and sex- matched controls; Determine thermal strain from skin and core body temperature in adults with CF on Kaftrio® during exercise under heat stress conditions and whether this is different to healthy age- weight- and sex- matched controls.

Official Title
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A Study to Assess the Sweat Response, Sweat Composition and Thermoregulatory Response to Exercise in Heat in Adults With Cystic Fibrosis on Cystic Fibrosis Transmembrane Conductance Regulator Modulator Therapy Compared Healthy Controls.

Conditions
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Cystic Fibrosis

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria for participants with CF: Is taking CFTRm Males and females ≥ 18 years of age CF diagnosis based on clinical features, supported by a history of an abnormal sweat test (sweat [Cl-] > 60 mmol·L-1 > 100 mg sweat), where possible, diagnostic genotyping would also be desired Can clearly state that they are not pregnant No contraindications to performing exhaustive exercise Can understand and cooperate with the study protocol No exacerbation (increase in cough, sputum or breathlessness, or change in the colour of sputum) within the preceding 2 weeks No weight loss in the preceding 2 weeks Inclusion Criteria for healthy control participants: Healthy males and females who are age- weight- and sex- matched to the enrolled individuals with CF Can clearly state that they are not pregnant No clinical diagnosis of a chronic disease Can understand and cooperate with the study protocol No contraindications to performing exhaustive exercise Exclusion Criteria for participants with CF: Any non-pulmonary conditions that may impair exercise ability, such as musculoskeletal disorders (arthritis, joint or muscle disease) and cardiovascular disease (congenital heart disease or cardiomyopathy). Unstable co-morbid asthma (daily pulmonary function variability of >20%) Is pregnant during the initial screening process Unable to understand or cooperate with the study protocol due to learning difficulties or otherwise Not of a suitable age for testing Is a smoker or inhales any other substances Exclusion Criteria for healthy control participants: Any pulmonary, metabolic or cardiovascular conditions Any other diagnosed disease Any non-pulmonary conditions that may impair exercise ability, such as musculoskeletal disorders (active arthritis, joint or muscle disease) and cardiovascular disease (congenital heart disease or cardiomyopathy) Is pregnant during the initial screening process Presents with co-morbidities to performing exhaustive exercise Is a smoker or inhales any other substances Unable to understand or cooperate with the study protocol due to learning difficulties or otherwise Not an age- weight- or sex-match for the CF group

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Cystic fibrosis<br>No intervention - only assessments | |
| Healthy Control<br>No intervention- only assessments | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Sweat sodium concentration (mmol∙L-1) | Between group differences in sweat sodium concentration | Day 2 - baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Whole-body sweat rate (mL) | Between group differences in whole-body sweat rate derived from pre - post nude body mass | Day 2 - baseline | 
| Forearm sweat rate (mg∙min-1∙cm2) | Between group differences in forearm sweat rate | Day 2 - baseline | 
| Mid-back sweat rate (mg∙min-1∙cm2) | Between group differences in mid-back sweat rate | Day 2 - baseline | 
| Sweat gland activity (gland∙cm2) | Between group differences in sweat-gland activation derived from the modified-iodine patch technique | Day 2 - baseline | 
| Core temperature (°C) | Between group differences in core temperature derived from rectal temperature | Day 2 - baseline | 
| Mean skin temperature (°C) | Between group differences in skin temperature | Day 2 - baseline | 
| Heart rate (b∙min-1) | Between group differences in heart rate | Day 2 - baseline | 
| Cutaneous blood flux | Between group differences in cutaneous blood flux | Day 2 - baseline | 
| Urine osmolality (mOsmol/kg) | Between group differences in urine osmolality | Day 2 - baseline | 
| Perception of temperature sensation | Between group differences in perception of temperature sensation | Day 2 - baseline | 
| Perception of thermal comfort | Between group differences in perception of thermal comfort | Day 2 - baseline | 
| Change in thermal comfort | Between group differences in change in thermal comfort | Day 2 - baseline | 
| Rate of change in thermal comfort | Between group differences in rate of change in thermal comfort | Day 2 - baseline | 
| Heat sensitivity | Between group differences in heat sensitivity derived from sweat rate / change in thermal comfort | Day 2 - baseline | 
| Heat illness symptom index | Between group differences in heat-related illness symptoms | Day 2 - baseline | 
| Maximal oxygen uptake (VO2 max) | Between group differences in oxygen uptake derived from a maximal cardiopulmonary exercise test | Day 1 - baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Exercise, CFTR modulator therapy, thermoregulation, heat-related illness, sweat sodium