Investigation of the Learning Effect in the Measurement of Circulation in Calf Muscle Using MSOT

Study Overview
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Brief Summary
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The goal of this observational study is to investigate the learning effect in the measurement of micro-circulation in healthy participants using MSOT. The main question it aims to answer are: If there if a learning effect in MSOT measurement. How long does it takes for the trainees to reach the professional level. Is there a difference between different learning methods? Participants will have to lie on the examination bed while investigator making measurements 7 times in a row on one single location.

Detailed Description
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Learning effect of MSOT measurements have been observed by previous studies. Therefore, in this study, we aim to quantify the learning effect with circulation-related parameters (Hb, HbO2, SO2) and the similarity between repeated images (structural similarity index measure, SSIM). The measurement would be repeated by 5 different investigators, two of them are professionals, defined as experience over 200 times of MSOT measurement, while the other three are trainees, who haven't performed any MSOT measurements before and each has received one different teaching method, one receiving personal teaching from a professional, one receiving video instruction and one receiving instructions written in paper form.

Official Title
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Investigation of the Learning Effect in the Measurement of Circulation in Calf Muscle Using the Multispectral Optoacoustic Tomography

Conditions
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Multispectral Optoacoustic Tomography, Learning Curve, Image Interpretation, Computer-Assisted, Structural Similarity Index Measure

Intervention / Treatment
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* Device: MSOT measurements


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: -all those who with the age between 18 and 40 year-old Exclusion Criteria: diabetes mellitus hypertension cardiovascular diseases chronic kidney diseases amputation in either lower extremities tatoos in either lower extremities

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Professional<br>Two investigators who have performed over 200 MSOT measurements. | Device: MSOT measurements<br>* Non-invasive transcutaneous imaging of subcellular muscle components via infrared and near-infrared laser pulses<br>|
| Trainee-personal teaching<br>Trainees are defined as who haven't performed any MSOT measurement. In this cohort, one trainee received a personal teaching from one professional. | Device: MSOT measurements<br>* Non-invasive transcutaneous imaging of subcellular muscle components via infrared and near-infrared laser pulses<br>|
| Trainee-video<br>Trainees are defined as who haven't performed any MSOT measurement. In this cohort, one trainee received instruction from video. | Device: MSOT measurements<br>* Non-invasive transcutaneous imaging of subcellular muscle components via infrared and near-infrared laser pulses<br>|
| Trainee-instruction in paperform<br>Trainees are defined as who haven't performed any MSOT measurement. In this cohort, one trainee received instruction in paperform. | Device: MSOT measurements<br>* Non-invasive transcutaneous imaging of subcellular muscle components via infrared and near-infrared laser pulses<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Learning curve in MSOT measurements. | If the quality of MSOT measurements improved with practices. | 5 days | 
| Different learning curve between professionals and trainees. | Observe the possible difference between the learning curves in professionals and trainees. | 5 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Different learning curve in three different teaching methods | Three trainees, each received different teaching methods, perform the same measurement on the same participants. The outcome is to observe possible difference in learning curve in these three groups. | 5 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Multispectral Optoacoustic Tomography, Learning Curve, Learning Effect, MSOT, Image Interpretation, Structural Similarity Index Measure, SSIM