Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In- Vitro Study

Study Overview
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Brief Summary
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The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. It has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis. The present study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability. It will be assessed using a scratch assay, Mtt assay and ATP analysis.

Detailed Description
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Introduction: Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis. Aim: This study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability. Methodology: In this in-vitro study, 20 Osteoblast - like cells will be included. 10 samples to be considered for Control group and the remaining 10 samples considered as Test group. Extracorporeal shockwave will be applied to the Test samples. They will be further analysed using wound scratch assay which is used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay used to determine Cell viability after exposure to shockwaves. The cell migration and cell viability after exposure to shockwaves will determine its effects on osteoblast-like cells.

Official Title
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Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In- Vitro Study

Conditions
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Osteosarcoma

Intervention / Treatment
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* Device: Extracorporeal shockwave device
* Device: No ESWT


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Cell cultures with a confluency of 70%-80% Exclusion Criteria: Contaminated cell cultures
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: test group - application of shockwave on osteoblast control group - No shockwave on osteoblast
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Shockwave treated group<br>The osteoblast like cells are treated with shockwaves | Device: Extracorporeal shockwave device<br>* Extracorporeal shockwave device generates true shock waves which are characterized by a sudden rise in pressure followed by an exponential decay. This is different from the commercially available shockwave devices in which they use piezoelectric based mechanisms which generate high frequency waves.<br>* Other names: ESWT;|
| Placebo Comparator: No shockwave group<br>The osteoblast like cells are not exposed to shockwaves | Device: No ESWT<br>* the osteoblast like cells are not exposed to shockwaves<br>* Other names: shockwave;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Scratch assay | cell migration | 72hours | 
| MTT Assay and ATP analysis | Cell viability | 74hrs | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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ESWT, Cell proliferation, Cell viability