Multiomic Analysis of Traumatic Brain Injury and Hypertension Intracranial Hemorrhage Lesion Tissue

Study Overview
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Brief Summary
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The goal of this experimental observation study is to figure out differently expressed biomarkers in lesion tissues in traumatic brain injury or hypertension intracranial hemorrhage patients. The main questions it aims to answer is: Which RNA, protein and metabolites are differently expressed in lesion tissues? What molecular mechanism is participated in TBI or ICH? Participants will be treated by emergency operation, and their lesion tissues will be collected during the operation.

Official Title
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Multiomic Analysis of Traumatic Brain Injury and Hypertension Intracranial Hemorrhage Lesion Tissue

Conditions
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Brain Injury Traumatic Severe, Intracranial Hemorrhage, Hypertensive

Intervention / Treatment
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* Other: Type of disease


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age >18 and <65 Within 12 hours after brain injury GCS ≥3 and ≤8 Closed brain injury with frontotemporal contusion or basal ganglia intracranial hemorrhage Need emergency intracranial hematoma evacuating operation Patient's agents are informed and consent the research Exclusion Criteria: With TBI, stroke, ICH or intracranial tumor history Death within 24 hours Immunosuppression state Severe organ dysfunction Complicated infective disease Pregnant

Ages Eligible for Study
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Minimum Age: 16 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| TBI<br>Patients with traumatic brain injury. | Other: Type of disease<br>* Different types of pathogen: brain injury by trauma or intracranial hemorrhage due to hypertension.<br>|
| ICH<br>Patients with hypertensive intracranial hemorrhage. | Other: Type of disease<br>* Different types of pathogen: brain injury by trauma or intracranial hemorrhage due to hypertension.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| RNA biomarkers in TBI or ICH patients' lesion tissues assessed by transcriptome bioinformatic analysis | Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed by bioinformatic analysis to reveal different RNA expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers. | Immediately after collecting tissues | 
| Protein biomarkers in TBI or ICH patients' lesion tissues assessed by proteomic bioinformatic analysis | Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed by bioinformatic analysis to reveal different protein expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers. | Immediately after collecting tissues | 
| Metabolite biomarkers in TBI or ICH patients' lesion tissues assessed by metabolomic bioinformatic analysis | Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed by bioinformatic analysis to reveal different metabolite expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers. | Immediately after collecting tissues | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Traumatic brain injury, Hypertensive intracranial hemorrhage, Multiomic analysis, Biomarker, Molecular mechanism