Multiomic Analysis of Body Fluid in Acute Traumatic Brain Injury

Study Overview
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Brief Summary
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The goal of this experimental observation study is to figure out differently expressed biomarkers in body fluid in traumatic brain injury patients. The main questions it aims to answer is: Which RNA, protein and metabolites are differently expressed in lesion tissues? What molecular mechanism is participated in TBI? Participants will be treated by emergency operation, and their body fluid samples will be collected in emergency room and during the operation.

Official Title
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Multiomic Analysis of Body Fluid in Acute Traumatic Brain Injury

Conditions
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Brain Injuries, Traumatic

Intervention / Treatment
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* Other: Type of disease


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age >18 and <65 Within 12 hours after brain injury Control group is hydrocephalus GCS ≥3 and ≤8 Closed brain injury with frontotemporal contusion Need emergency intracranial hematoma evacuating or cerebrospinal fluid shunting operation Patient's agents are informed and consent the research Exclusion Criteria: With TBI, stroke, ICH or intracranial tumor history Death within 24 hours Immunosuppression state Severe organ dysfunction Complicated infective disease Pregnant

Ages Eligible for Study
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Minimum Age: 16 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| TBI<br>Patients with traumatic brain injury. | Other: Type of disease<br>* Different types of pathogen: brain injury by trauma or hydrocephalus due to various diseases.<br>|
| Control<br>Patients with hydrocephalus. | Other: Type of disease<br>* Different types of pathogen: brain injury by trauma or hydrocephalus due to various diseases.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| RNA biomarkers in patients' blood & cerebrospinal fluid assessed by transcriptome bioinformatic analysis | Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed and compared with control group to filter potential RNA biomarkers in blood and cerebrospinal fluid of severe TBI patients. | Immediately after collecting blood & cerebrospinal fluid | 
| Protein biomarkers in patients' blood & cerebrospinal fluid assessed by proteomic bioinformatic analysis | Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed and compared with control group to filter potential protein biomarkers in blood and cerebrospinal fluid of severe TBI patients. | Immediately after collecting blood & cerebrospinal fluid | 
| Metabolite biomarkers in patients' blood & cerebrospinal fluid assessed by metabolomic bioinformatic analysis | Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed and compared with control group to filter potential metabolite biomarkers in blood and cerebrospinal fluid of severe TBI patients. | Immediately after collecting blood & cerebrospinal fluid | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Traumatic brain injury, Body fluid, Biomarker, Multiomic analysis