Resistance Training on Growth Factors

Study Overview
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Brief Summary
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The goal of this clinial trial study is to explore the effect of high or low intensity resistance training (LIRT) under similar training volume on change in serum levels of BDNF, IGF-1, VEGF, irisin, and plasma Hcy in young adults.The main questions it aims to answer are: Whether high and low intensity will increase the serum levels of BDNF, IGF-1, VEGF, irisin, and reduce plasma Hcy. whether a greater magnitude of change would be suffer in low intensity group when compared to high intensity group, as an effect of greater physiological adaptation produced by more repetitions required to complete. Participants will performe two different intensity resistance training (HIRT, at 80%1RM with 12 repetitions and 4-5 sets and LIRT, at 40%1RM with 24 repetitions and 4-5 sets). Researchers will compare the training effects of two different training on these growth factors.

Official Title
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Acute Effect of Resistance Training With Different Intensity on Circulating Levels of Neurotrophic Growth Factors and Homocysteine in Young Adults

Conditions
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Cognitive Function

Intervention / Treatment
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* Other: High intensity resistance training
* Other: Low intensity resistance training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age between 18 to 30 years no experiencing musculoskeletal disorders or sports injuries had not participated any regular aerobic endurance or resistance training with in the previous half years no smoking habit or intake alcohol and other medication (e.g., steroid hormone) good general body condition without any chronic diseases (e.g., osteoarthritis, metabolic syndrome or arthritis) Exclusion Criteria: experiencing musculoskeletal disorders or sports injuries in recent 1 month had regular resistance training exercise intake any supplementary(drug and hormone)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: High-intensity resistance training group (HIRT)<br>All subjects were instructed to conduct HIRT consisted of 4-5 sets of 12 repetitions at 80%1RM, with 1-2 minutes rests between each set. | Other: High intensity resistance training<br>* The HIRT protocol comprised five exercises (bench press, barbell back squat, deadlift, seated leg flexion, and reverse arm curl) at 80% of 1RM, and 4-5 sets of 12 repetitions with 1-2 minutes of rest period between sets for approximately 60 minutes.<br>|
| Experimental: low-intensity resistance training group (LIRT)<br>All subjects were instructed to conduct LIRT consisted of 4-5 sets of 24 repetitions at 40%1RM, with same period) in random order. | Other: Low intensity resistance training<br>* For LIRT protocol, which performed same training exercises as HIRT. The repetitions in the LIRT protocol were assessed using the following equation: 80% of 1RM lifting load (kg) ×repetitions (HIRT)/40% of 1RM to volition fatigue, with 1-2 minutes of rest period between sets. Therefore, the LIRT protocol completed 4-5 sets of 24 repetitions at 40% of 1RM for a total of 60 minutes.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| rata of perceived exertion | The rating of perceive exertion (RPE) questionnaire scale was applied to assess participants' subjective fatigue feeling throughout the training period. | pre,post immediately, 30min post | 
| blood lactate | serum blood lactate | pre, post immediately, 30 minutes post intervention | 
| BDNF | regulators of neurogenesis | pre, post immediately, 30 minutes post intervention | 
| IGF-1 | regulators of neurogenesis | pre, post immediately, 30 minutes post intervention | 
| VEGF | regulators of neurogenesis | pre, post immediately, 30 minutes post intervention | 
| Irisin | regulators of neurogenesis | pre, post immediately, 30 minutes post intervention | 
| Hcy | homocysteine (Hcy) increase with age and resulted in cognitive function decline through greater oxidative stress and vascular dysfunction | pre, post immediately, 30 minutes post intervention | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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neurotrophic growth factors, IGF-1, BDNF, VEGF, Irisin, Hcy