The Effects of Dexmedetomidine on Thiol/Disulphide Homeostasis in Coronary Bypass Surgery

Study Overview
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Brief Summary
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Dexmedetomidine (DEX) is a sedative and anesthetic drug with known antioxidant properties.In this study, it was aimed to investigate the oxidative stress levels in patients undergoing on-pump CABG by measuring the thiol/disulfide levels and to investigate the effects of dexmedetomidine infusion used in Coronary Bypass Surgery on dynamic Thiol/Disulphide Homeostasis.

Detailed Description
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It is well known that cardiovascular disease is the leading cause of increased morbidity and mortality worldwide. One of the key surgical procedures that patients with coronary artery disease (CAD) are often subjected to is coronary artery bypass grafting (CABG). However, the cardiopulmonary bypass (CPB) pump increases systemic oxidative stress . High levels of molecular oxygen enter the body during cardiopulmonary bypass (CPB) and reperfusion, followed by inflammation leading to the formation of free oxygen radicals which causes lipid peroxidation, ultimately resulting in an oxidative attack at the cell and tissue level. Thiols are organic sulphur derivatives containing Sulfhydryl Residues (-SH) in their active regions. Thiols easily react with oxygen containing free radicals to form disulfides. This is a defence mechanism against oxidative stress.7 An automated analysis quantitatively measuring serum native and total thiol, and disulfides has been recently described as a method to determine dynamic Thiol/Disulfide Homeostasis (TDH).The role of dynamic thioldisulfide homeostasis has been increasingly shown in many diseases. There is a growing number of evidences that an abnormal thiol-disulfide homeostasis may play role in the pathogenesis of a variety of diseases such as cardiovascular disease, malignancies, rheumatoid arthritis, chronic kidney disease, and acquired immunodeficiency syndrome. Dexmedetomidine (DEX) is a sedative and anesthetic drug with known antioxidant properties.In this study, it was aimed to investigate the oxidative stress levels in patients undergoing on-pump CABG by measuring the thiol/disulfide levels and to investigate the effects of dexmedetomidine infusion used in Coronary Bypass Surgery on dynamic Thiol/Disulphide Homeostasis.

Official Title
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The Effects of Dexmedetomidine on Thiol/Disulphide Homeostasis in Coronary Bypass Surgery

Conditions
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Dexmedetomidine, Oxidative Stress

Intervention / Treatment
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* Diagnostic Test: Thiol/Disulphide Homeostasis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients who have undergone coronary bypass surgery using a cardiopulmonary pump Patients who agreed to participate in the study Exclusion Criteria: Bypass surgeries without cardiopulmonary pump Patients with intraoperative unexpected/undesirable complications Patients who did not agree to participate in the study Patients with missing data

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Dexmedetomidine used group in Coronary Bypass Surgery<br> | Diagnostic Test: Thiol/Disulphide Homeostasis<br>* An automated analysis quantitatively measuring serum native and total thiol, and disulfides has been recently described as a method to determine dynamic Thiol/Disulfide Homeostasis (TDH).<br>|
| Dexmedetomidine non-used group in Coronary Bypass Surgery<br> | Diagnostic Test: Thiol/Disulphide Homeostasis<br>* An automated analysis quantitatively measuring serum native and total thiol, and disulfides has been recently described as a method to determine dynamic Thiol/Disulfide Homeostasis (TDH).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Thiol/Disulphide Homeostasis | An automated analysis quantitatively measuring serum native and total thiol, and disulfides has been recently described as a method to determine dynamic Thiol/Disulfide Homeostasis (TDH). | 30 min before the start of surgery | 
| Thiol/Disulphide Homeostasis | An automated analysis quantitatively measuring serum native and total thiol, and disulfides has been recently described as a method to determine dynamic Thiol/Disulfide Homeostasis (TDH). | at the time of aortic cross clamp off | 
| Thiol/Disulphide Homeostasis | An automated analysis quantitatively measuring serum native and total thiol, and disulfides has been recently described as a method to determine dynamic Thiol/Disulfide Homeostasis (TDH). | at the time of surgery over | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Disulphide, Thiol