GCF miRNA-223 , -214 Levels in Smokers and Nonsmokers Periodontitis Patients

Study Overview
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Brief Summary
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Objective: Periodontitis is a multifactorial disease that affects a wide range of populations. However, its pathogenesis remains unclear. miRNAs are now considered potential diagnostic markers for many inflammatory diseases. Thus, the aim of this study was to assess the expression of microRNA-223(miRNA-223) and microRNA-214 (miRNA-214) in gingival crevicular fluid (GCF) of smoker and nonsmoker with periodontitis. Materials and Methods: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smoker with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.

Official Title
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Expression of microRNA-223 and microRNA-214 in Gingival Crevicular Fluid of Smoker and Nonsmoker Periodontitis Patients, an Observational Diagnostic Accuracy Study

Conditions
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Periodontitis, Adult

Intervention / Treatment
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* Diagnostic Test: miRNA-223 & -214 detection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: both genders age from 20 to 60 years no more than 4 teeth lost due to periodontitis systemically free participants Exclusion Criteria: Pregnant or lactating female participants with a history of an inflammatory or immunological disease history of any current medication

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group I<br>14 healthy volunteers | Diagnostic Test: miRNA-223 & -214 detection<br>* miRNA-223 & -214 detection in GCF samples by PCR kits<br>|
| Group II<br>14 periodontitis participants with stage III grade B Periodontitis | Diagnostic Test: miRNA-223 & -214 detection<br>* miRNA-223 & -214 detection in GCF samples by PCR kits<br>|
| Group III<br>14 periodontitis participants with stage III grade C Periodontitis | Diagnostic Test: miRNA-223 & -214 detection<br>* miRNA-223 & -214 detection in GCF samples by PCR kits<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| assessment of miRNA-223 & -214 levels in GCF samples in all groups | PCR kits are used for assessment of miRNA-223 & -214 levels in GCF samples in all groups | level of miR-223 & -214 are measured at baseline after the completion of all GCF sample collection carried at the day of enrollment of subjects in the investigation after diagnosis confirmation | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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biofluids, Periodontitis, microRNA, smoking, GCF