Fetuin A Association in Periodontal Disease and Diabetes Mellitus

Study Overview
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Brief Summary
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Aim: The aim of the current study was to compare and correlate gingival crevicular fluid (GCF) fetuin-A levels and high sensitivity C-reactive protein (hs-CRP) levels in non-diabetic periodontitis patients and type 2 diabetic periodontitis patients with healthy subjects and revealing its possible role in diagnosis of periodontal disease. Methodology: Forty-five periodontitis stage III patients categorized into 3 groups according to whether having type II diabetes mellitus or not were compared to systemically and periodontally healthy participants in this investigation. Clinical parameters for all groups were recorded. Assessment of GCF levels of fetuin A and hs-CRP were done to all enrolled participants utilizing an enzyme linked immune-sorbent assay (ELISA) kit. Receiver operating characteristic (ROC) curve was performed to reveal sensitivity, specificity, predictive value and diagnostic accuracy of the markers used.

Official Title
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Evaluation of Fetuin-A and hsCRP Levels in Gingival Crevicular Fluid in Periodontitis Diabetic and Non-Diabetic Patients

Conditions
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Periodontitis, Adult

Intervention / Treatment
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* Diagnostic Test: GCF levels of fetuin A and hsCRP evaluation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: both gender periodontitis stage III Exclusion Criteria: Smoking, Systemic diseases (except DM in group B), Periodontitis patients having periodontal treatment recently (past 6 months), Pregnancy, lactation, contraceptive pills, Anti-inflammatory/ immunosuppressive drugs.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group I<br>15 healthy patients with generalized periodontitis stage III. | Diagnostic Test: GCF levels of fetuin A and hsCRP evaluation<br>* ELIZA kits are used for GCF levels of fetuin A and hsCRP evaluation<br>|
| Group II<br>15 patients suffering from type 2 diabetes mellitus with generalized periodontitis stage III | Diagnostic Test: GCF levels of fetuin A and hsCRP evaluation<br>* ELIZA kits are used for GCF levels of fetuin A and hsCRP evaluation<br>|
| Group III<br>15 systemically healthy patients without periodontitis | Diagnostic Test: GCF levels of fetuin A and hsCRP evaluation<br>* ELIZA kits are used for GCF levels of fetuin A and hsCRP evaluation<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| GCF levels of fetuin A and hsCRP evaluation in all groups | ELIZA kits | levels of fetuin A and hsCRP are measured at baseline after the completion of all GCF sample collection carried at the day of enrollment of subjects in the investigation after diagnosis confirmation |