Role of IL-5R Signaling in Non-eosinophil Upper Airway Cells in CRSwNP

Study Overview
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Brief Summary
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The overarching hypothesis of this proposal is that IL-5 acts on multiple sinus tissue cell types, including plasma cells and epithelial cells, to promote immune dysregulation, and that inhibition of IL-5 affects several relevant effector pathways that lead to clinical benefit.

Detailed Description
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Further characterization of the role of IL-5Rα expression and function on human plasma cells will determine whether inhibiting IL-5 signaling on these cells offers therapeutic promise in other diseases related to plasma cell proliferation. Further characterization of human upper airway epithelial cell IL-5Rα expression and function will determine whether or not inhibiting IL-5 signaling on these cells offers therapeutic promise in other diseases related to epithelial dysfunction. IL-5Rα expression has been identified on several relevant sinus tissue effector cells, including on nasal polyp plasma cells and epithelial cells, and the aim of this study is to further the field by determining the consequences of IL-5 signaling on those cells.

Official Title
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Role of Interleukin 5 (IL-5) and IL-5R Signaling in Non-eosinophil Upper Airway Cells in Chronic Rhinosinusitis With Nasal Polyps

Conditions
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Nasal Polyps, Aspirin-exacerbated Respiratory Disease

Intervention / Treatment
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* Other: Non-Interventional


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: AERD, CRSwNP, healthy controls Exclusion Criteria: Not on any systemic steroids or respiratory biologics at time of surgery

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Aspirin-exacerbated respiratory disease (AERD)<br>Aspirin-exacerbated respiratory disease (AERD) | Other: Non-Interventional<br>* No intervention.<br>|
| Aspirin-tolerant chronic rhinosinusitis with nasal polyps (CRSwNP)<br>Aspirin-tolerant chronic rhinosinusitis with nasal polyps (CRSwNP) | Other: Non-Interventional<br>* No intervention.<br>|
| Healthy controls<br>Individuals who do not have AERD or CRSwNP | Other: Non-Interventional<br>* No intervention.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Nasal polyp plasma cell transcriptome | Transcriptional profile of nasal polyp plasma cells in nasal polyps from patients with AERD and aspirin-tolerant CRSwNP | At baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Function of IL-5Ra on nasal polyp plasma cells. | Functional significance of IL-5Rα expression and IL-5 signaling on the plasma cells in nasal polyp tissue | At baseline | 
| IL-5Ra signaling on nasal epithelial cells | Define the IL-5-elicited molecular features of upper airway nasal epithelial tissue from subjects with healthy sinus mucosa, CRSwNP, and AERD. | At baseline |