Oral Microbiota and Oral Status of Adult Patients With Autism Spectrum Disorders (MICAA: MICrobiota Autism Adults)

Study Overview
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Brief Summary
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The goals of this pilot study are to assess the presence of harmful germs for gums and to assess oral health in adults with autism spectrum disorder.

Detailed Description
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Participants or their tutors will fulfill a form with general health information and daily habits. Investigators will : collect saliva, gum fluid and/ or dental plaque to characterise germs assess gum health and oral hygiene register decays, filled and missing teeth.

Official Title
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Characterisation of the Oral Microbiota and Oral Status of a Population of Adult Patients With Autism Spectrum Disorders: a Pilot Study

Conditions
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Autism Spectrum Disorder

Intervention / Treatment
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* Other: collection of saliva, gum fluid and/or dental plaque samples


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: A person (or legal representative) who has received full information about the organisation of the research and accept to participate and the use of his or her data. Adult with autism spectrum disorder, men or women aged between 18 and 60. Member of a social security scheme or beneficiary of a medical insurance Exclusion Criteria: Children under 18 with autism spectrum disorder Use of medication or situations that may affect the microbiota (use of antibiotics, probiotics, steroidal or non-steroidal anti-inflammatory drugs in the last 3 months, strict mouth breathing) Smoking Very lingual position of the teeth hindering clinical assessment of the periodontium Patients who have had periodontal treatment for less than 6 months Persons covered by articles L. 1121-5 to L. 1121-7 and L1122-2 of the French Public Health Code: Pregnant women, women in labour or breast-feeding mothers Persons deprived of their liberty by a judicial or administrative decision Persons unable to give their consent Person under psychiatric care by virtue of articles L. 3212-1 and L. 3213-1 of the Public Health Code Minor (not emancipated)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Intervention Model: Single Group Assignment
Interventional Model Description: Pilot study concerning adults with autism spectrum disorder
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Adults with Autism Spectrum Disorder<br>Adults with autism spectrum disorder Aged between 18 and 60 years old Dental cares and follow-up in Nancy hospital | Other: collection of saliva, gum fluid and/or dental plaque samples<br>* Non-invasive sampling of dental plaque, crevicular fluid and/or saliva, using an examination probe, paper tips and saliva swabs<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Presence of periodontopathogenic bacteria (harmful for gums) | Technique used = Polymerase Chain Reaction (PCR) Measure unit = Colony-Forming Unit (CFU) | Collection on Day 1 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Assessment of the presence of parasites Entamoeba gingivalis subtype 1 (ST1) and subtype 2 (ST2) and Trichomonas tenax | Technique = Polymerase Chain Reaction (PCR) Unit = Colony-Forming Unit (CFU) | Collection on Day 1 | 
| Assessment of the periodontal status of patients with Autism Spectrum Disorder | Index used = the Dutch Periodontal Index 0 = minimum value, better outcome 4 = maximum value, worse outcome | Day 1 | 
| Assessment of the dental condition | Dental data will be collected using the Decay-Missing-Filled/Teeth (DMFT) score 0 = minimum value, best outcome 28 = maximum value, worst outcome | Day 1 | 
| Assessment of oral hygiene | Oral hygiene will be assessed using the Silness and Löe plaque index and the lifestyle questionnaire 0 = minimum value, best outcome 3 = maximum value, worst outcome | Day 1 | 
| Collection of data about lifestyle habits (oral and dental hygiene, diet, activity, etc.) and drug treatments that may influence the clinical parameters observed | Data collection via a form fulfilled by the participant or the patient's legal guardian | Before samples collection and clinical examination | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Autism Spectrum Disorder, Oral microbiota, Oral Health