The Influence of Endurance Sports Activity on the Oral Microbiota of Young Adults.

Study Overview
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Brief Summary
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The oral cavity is an easily accessible anatomical site that accurately reflects the health of the individual. The mouth is considered an early indicator of biological aging, leading to the identification of oral biomarkers predictive of future alterations. Current data show that the oral health of high level athletes is globally altered with an increase in cariogenic indexes, an increased incidence of periodontitis, dental trauma and dry mouth syndromes. Few studies have focused on the oral microbiota compared to mouthguards used in rugby or boxing which seem to induce dysbiosis, a recent study shows an effect of ketogenic diet on bacterial changes in the mouth of endurance athletes. This project focuses on the impact of intensive sport practice on oral health and the identification of oral biomarkers associated with physical activity. The objective of this study is to investigate the impact of the amount of endurance sport practice on oral microbiota and oral health in young adults. the hypothesis is that a sedentary lifestyle and high-level sport induce depletion and/or changes in the oral microbiota compared to regular physical activity.

Official Title
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Sport and Health a Pilot Study: Impact of Endurance Physical Activity on the Microbiological Homeostasis of the Oral Cavity of Young Adults

Conditions
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Microbial Colonization

Intervention / Treatment
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* Other: salivar and gum sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Be between 18 and 30 years old If practicing sports, it must be an endurance sport as main activity (cycling or running) Exclusion Criteria: Practice more than 3 hours of swimming per week Have taken anti-inflammatory drugs, less than 48 hours before the study Have taken antibiotics and/or probiotics, less than 3 weeks before the study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: sedentary<br>Participant does not meet WHO's recommendations with the physical activity | Other: salivar and gum sampling<br>* Saliva will then be collected by salivary spit and a mucosal smear will be taken using a swab passed at the junction of the tooth and the gum<br>|
| Other: OMS recommandations<br>Participant does not meet WHO's recommendations (2.5-5 hours per week of moderate physical activity OR 1.25-2.5 hours per week of vigorous physical activity); | Other: salivar and gum sampling<br>* Saliva will then be collected by salivary spit and a mucosal smear will be taken using a swab passed at the junction of the tooth and the gum<br>|
| Other: More than the OMS recommandations<br>Participant is practicing physical activity more than the WHO recommendations | Other: salivar and gum sampling<br>* Saliva will then be collected by salivary spit and a mucosal smear will be taken using a swab passed at the junction of the tooth and the gum<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Analysis of the oral microbiota in healthy individuals engaged in endurance physical activity at different levels. | Microbiological screening will be performed using an innovative methodology based on microfluidic PCR (BiomarkHD) (number of bacterial colonies) | 3 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Quantification of salivary inflammatory markers in healthy individuals who perform endurance physical activity at different levels. | numer and type of salivary inflammatory markers will performed using multiplex analysis technology developed by the firm Meso Scale Diagnostic (MSD). | 3 years |