Dose Response Relationship Between Fat Ingestion and Metabolism

Study Overview
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Brief Summary
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The goal of this clinical trial is to compare features of metabolism in healthy, young adults after they consume four meals of differing fat quantity. The main question this trial aims to answer is how does increasing fat quantity impact glucose tolerance, glucose and insulin metabolism, and hormones involved in hunger. Participant will consume four meals consisting of either 20, 40, 60, or 80% energy from fat.

Detailed Description
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The long-term consumption of high-fat foods can lead to unhealthy changes in the functioning of the body, giving rise to metabolic diseases such as type 2 diabetes. Similarly, short-term high-fat diets have also been shown to disrupt how the body performs but there is a lack of information regarding how a single high-fat meal can disrupt metabolism. Comparing meals of different fat quantity, without changing the overall energy intake (calories), will help us understand the short-term effect of fat ingestion on the functioning of the body and whether increasing the amount of fat in a meal is related to the level of dysfunction in the body. Therefore, the investigators will provide participants with four meals of varying fat quantity and in the period following the meal, the investigators will take blood samples to determine any changes that occur in the body in response to different levels of fat.

Official Title
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Investigating the Effect of Acute Fat Ingestion on Glucose Metabolism in Young Healthy Adults: a Dose Response Study

Conditions
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Glucose Tolerance Impaired

Intervention / Treatment
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* Other: 20% fat meal
* Other: 40% fat meal
* Other: 60% fat meal
* Other: 80% fat meal


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI between 18.5 and 30.0 kg/m2 Weight stable for the past 6 months (± 2kg) VO2max values within a below average to above average range (38-50 mL/kg and 35-47 mL/kg for 18-25 year old males and females respectively. 35-48 mL/kg and 34-45 mL/kg for 26-35 year old males and females respectively) Fasting blood glucose <6.0 mM Resting blood pressure <140/90 mmHg Taking second generation oral contraceptives (females only) Exclusion Criteria: Smoking Diabetes, cancer, or other metabolic disorders Cardiac or gastrointestinal problems Infectious disease Barium swallow or nuclear medicine scan in the previous 3 weeks Follow a strict vegan diet Pregnant or breastfeeding (females only) Diagnosis of polycystic ovary syndrome (females only)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Each participant will be required to consume four breakfast meals (containing 20, 40, 60, or 80% energy from fat) in a randomized order with a washout period between meals
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: 20% fat meal<br>This meal will contain 15 kcal/kg of body weight and consist of 20% fat, 65% carbohydrates, and 15% protein. | Other: 20% fat meal<br>* Provided to participants once throughout duration of study, contains 20% fat<br>|
| Experimental: 40% fat meal<br>This meal will contain 15 kcal/kg of body weight and consist of 40% fat, 45% carbohydrates, and 15% protein. | Other: 40% fat meal<br>* Provided to participants once throughout duration of study, contains 40% fat<br>|
| Experimental: 60% fat meal<br>This meal will contain 15 kcal/kg of body weight and consist of 60% fat, 25% carbohydrates, and 15% protein. | Other: 60% fat meal<br>* Provided to participants once throughout duration of study, contains 60% fat<br>|
| Experimental: 80% fat meal<br>This meal will contain 15 kcal/kg of body weight and consist of 80% fat, 5% carbohydrates, and 15% protein. | Other: 80% fat meal<br>* Provided to participants once throughout duration of study, contains 80% fat<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glucose tolerance | A 2-hour intravenous glucose tolerance test will be performed 4-hours after meal consumption | Two hours (taking place following the 4-hour postprandial period) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glucose and lipid metabolites | Postprandial availability of glucose (mM) | During the 4-hour postprandial period | 
| Glucose and lipid metabolites | Postprandial availability of insulin (µIU/mL) | During the 4-hour postprandial period | 
| Glucose and lipid metabolites | Postprandial availability of total cholesterol (mmol/L) | During the 4-hour postprandial period | 
| Glucose and lipid metabolites | Postprandial availability of low-density lipoprotein (mmol/L) | During the 4-hour postprandial period | 
| Glucose and lipid metabolites | Postprandial availability of high-density lipoprotein (mmol/L) | During the 4-hour postprandial period | 
| Glucose and lipid metabolites | Postprandial availability of non-esterified fatty acids (mmol/L) | During the 4-hour postprandial period | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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High-fat, Glucose, Insulin, Metabolism, Nutrition