ESWT on Human Gingival Fibroblasts

Study Overview
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Brief Summary
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The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis. The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, these cells were assessed for Cell - Cell interaction and Cell Viability. In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed

Detailed Description
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BACKGROUND: Improved understanding of human biology at the molecular level, scientific and technologic advances are emerging aspects in dentistry. The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis. AIM: The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival tissue derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, fibroblast cells were assessed for Cell - Cell interaction and Cell Viability. MATERIALS AND METHODS: In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed using wound scratch assay which was used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay was used to determine Cell viability after exposure to shockwaves.

Official Title
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Application of Extracorporeal Shockwaves on Human Gingival Fibroblasts in Vitro Study

Conditions
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Periodontal Attachment Loss

Intervention / Treatment
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* Device: extracorporeal shockwave device
* Other: Fibroblast cell lines not subjected to shockwaves from extracorporeal shockwave device


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Viable human gingival fibroblast Healthy gingival tissue Exclusion Criteria: contaminated samples
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Health Services Research
Allocation: Non-Randomized
Intervention Model: Sequential Assignment
Interventional Model Description: PCS-201-018 Fibroblast cell line (n=10) would be exposed to shockwave generator depending on Test group. Control group Fibroblast cell line (n=10) would be assessed without shockwave exposure. Cell proliferation and Cell viability will be assessed for both Test and Control Fibroblast cell lines using Scratch assay, MTT analysis and ATP analysis, accordingly.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Shockwave treated group<br>Effect of Extracorporeal shockwaves on Fibroblast cell proliferation and cell viability | Device: extracorporeal shockwave device<br>* Fibroblast cell lines (n=10) were subjected to Shockwaves from extracorporeal shockwave device<br>|
| Placebo Comparator: No shockwave group<br>Fibroblast cell lines assessed for proliferative rate and viability without shockwave exposure. | Other: Fibroblast cell lines not subjected to shockwaves from extracorporeal shockwave device<br>* Fibroblast cell lines (n=10) without shockwave exposure<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Fibroblast cell lines exposed to shockwave from extracorporeal shockwave device | Cell proliferation and Cell viability | 74 hrs | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Cell culture, ESWT, Cell proliferation, Cell viability