The Effect of Sugar Load in IBS Patients Depending on Sucrase-isomaltase Genes

Study Overview
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Brief Summary
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The investigators know that many patients with irritable bowel syndrome (IBS) have functional variants of genes coding for sucrase-isomaltase enzymes. The investigators will now examine whether these functional variants are associated with defect degradation of sucrose and associated gastrointestinal symptoms

Detailed Description
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The investigators have in a previous study included IBS patients for treatment with a starch- and sucrose reduced diet (SSRD). The investigators found a great improvement of symptoms. The investigators gene-tested those patients, and now have the full identification of all functional variants encoding sucrase-isomaltase enzymes. The investigators will invite patients from this previous study with normal genes and functional variants of genes. The patients will come fasting over night to the department of clinical research. The participants will ingest 75 g sugar dissolved in 0.8 dl water and flavored with lemon, to ingest during 5 minutes. After that, glucose will determined repeatedly up to 2 hours afterwards. At the same time, the participants have to assess their gastrointestinal sympotms on visual analogue scales (VAS). The investigators will compare the raise in b-glucose and symptoms between the groups of patients, divided into gene expression.

Official Title
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Comparison of Sugar Load Between Normal and Functional Variants of Sucrase-isomaltase Genes in IBS

Conditions
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Sucrase-Isomaltase Deficiency, Irritable Bowel Syndrome

Intervention / Treatment
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* Dietary Supplement: Sugar


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Participants of our previous clinical trial with SSRD diet (NCT03306381) Exclusion Criteria: Difficulties in taking blood samples

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Intervention Model: Single Group Assignment
Interventional Model Description: Patients with normal genes and with functional variants of sucrase-isomaltase genes
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Sugar load<br>Sugar dissolved in water will be ingested during 5 minutes | Dietary Supplement: Sugar<br>* 75 g sugar dissolved in 0.8 dl water and flavored with lemon. Ingested during 5 minutes<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes from baseline of blood glucose levels | Measurement of glucose levels in blood drawn from an intravenous needle and analyzed at the Department of Clinical Chemostry | From distribution repeatedly up to 2 hours | 
| Change from baseline of gastrointestinal symptoms | Measurment of gastrointestinal symptoms on a visual analog scale measuring symptoms, range 0-100 mm, repeatedly at the same time points as blood glucose is measured. The higher scores, the worse symptoms. | From baseline and up to 2 hours, at the same time points as blood glucose is measured | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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gastrointestinal symptoms, glucose