Effect of a Physical Exercise Session Performed During a 24-34 Hour Fasting Period on Energy Metabolism and Cognitive Function in Healthy Adults

Study Overview
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Brief Summary
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This study explores the impact of high-intensity exercise during fasting on energy metabolism and cognitive function in healthy adults. Changes in respiratory exchange ratio, blood substances, and cognitive performance will be measured after 24 and 34 hours of fasting with and without exercise in a randomized crossover design.

Official Title
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Efecto de Una sesión de Ejercicio físico Realizada Durante un Periodo de Ayuno de 24-34 Horas Sobre el Metabolismo energético y la función Cognitiva en Adultos Sanos: Estudio Piloto

Conditions
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Energy Metabolism

Intervention / Treatment
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* Behavioral: 34-hour fasting in sedentary conditions
* Behavioral: 34-hour fasting with high-intensity exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18-40 years old. Body mass index: 18.5-30.0 Kg/m2. Stable body weight (5kg range within the last 3 months) Being able to consume all the food items provided as part of the study. Not having change the time zone or have shifted sleeping hours equivalent to more than 3 hours on the previous 2 weeks. Exclusion Criteria: Diagnosed with any chronic condition that, in the research team's opinion or based on the American College of Sports Medicine guidelines, could contraindicate the implementation of 34-hour fasting periods and/or high-intensity exercise. Diagnosed with a chronic endocrine, digestive, rheumatic, neurological, cardiac, pulmonary, autoimmune, or infectious condition that affects energy metabolism. Presenting any signs or symptoms of illness that, in the research team's opinion or based on the American College of Sports Medicine guidelines, could contraindicate the implementation of 34-hour fasting periods and/or high-intensity exercise. Pregnant or breastfeeding. Using medications or supplements that may alter energy metabolism. Currently undergoing or having undergone any type of nutritional intervention or treatment in the past 3 months. Engaging in more than 5 hours per week of habitual moderate-to-vigorous cycling. Any other condition that, in the research team's opinion, discourages participation in the study.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Fasting, then fasting+exercise<br> | Behavioral: 34-hour fasting in sedentary conditions<br>* Participants will fast for 34h after a standardized breakfast, refraining from physical activity.<br>Behavioral: 34-hour fasting with high-intensity exercise<br>* Participants will fast for 34h after a standardized breakfast, performing a high-intensity exercise session 6-10 hours aprox. after starting the fasting period.<br>|
| Experimental: Fasting + Exercise, then fasting<br> | Behavioral: 34-hour fasting in sedentary conditions<br>* Participants will fast for 34h after a standardized breakfast, refraining from physical activity.<br>Behavioral: 34-hour fasting with high-intensity exercise<br>* Participants will fast for 34h after a standardized breakfast, performing a high-intensity exercise session 6-10 hours aprox. after starting the fasting period.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Respiratory Exchange Ratio |  | 24-hour after starting the fasting period | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Respiratory Exchange Ratio |  | 34-hour after starting the fasting period | 
| Serum insulin concentration |  | 24-hour after starting the fasting period | 
| Serum insulin concentration |  | 34-hour after starting the fasting period | 
| Serum glucose concentration |  | 24-hour after starting the fasting period | 
| Serum glucose concentration |  | 34-hour after starting the fasting period | 
| Dimensional Change Card Sort Test (NIH toolbox) |  | 24-hour after starting the fasting period | 
| Dimensional Change Card Sort Test (NIH toolbox) |  | 34-hour after starting the fasting period | 
| List Sorting Working Memory Test (NIH toolbox) |  | 24-hour after starting the fasting period | 
| List Sorting Working Memory Test (NIH toolbox) |  | 34-hour after starting the fasting period | 
| Flanker Test (NIH toolbox) |  | 24-hour after starting the fasting period | 
| Flanker Test (NIH toolbox) |  | 34-hour after starting the fasting period |