The Effect of a Plant-blend Protein Isolate on Post-exercise Myofibrillar Protein Synthesis

Study Overview
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Brief Summary
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Dietary protein ingestion is required to maximise the anabolic response during the recovery from resistance exercise. Whey protein is considered the optimal dietary strategy to maximise post-exercise muscle protein synthesis, but animal-protein production and consumption is associated with growing environmental and ethical concerns. Plant-based protein sources are considered of lesser anabolic quality than isonitrogenous boluses of animal-derived protein attributed to, at least in part, deficiencies in key essential amino acid. Blending different protein sources may overcome amino acid deficiencies and potentiate the post-exercise anabolic response. In the present study the investigators assessed the post-exercise muscle protein synthetic response following the ingestion of a novel plant-based protein isolate when compared with an isonitrogenous bolus of whey protein in healthy young, resistance trained women and men.

Official Title
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The Effect of a Novel Plant-blend Protein Isolate on Post-exercise Myofibrillar Protein Synthesis When Compared to Whey Protein in Healthy Young Adults

Conditions
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Muscle Metabolism

Intervention / Treatment
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* Behavioral: Bilateral resistance exercise
* Dietary Supplement: Whey protein
* Dietary Supplement: Plant-blend protein


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI between 18 - 30 Resistance trained (>3 times per week structured resistance exercise training for at least 3 months) Exclusion Criteria: Any metabolic impairment Any cardiovascular impairment Smoking Lactose intolerance Allergies to products containing dairy, meat or nuts Prescribed intake of over the counter pharmaceuticals

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Whey protein isolate<br>Following a bout of bilateral resistance exercise, participants will ingest 32 g protein from whey protein isolate | Behavioral: Bilateral resistance exercise<br>* A bout of bilateral resistance exercise consisting of barbell back squat, leg leg press and leg extension<br>Dietary Supplement: Whey protein<br>* Following execution of bilateral resistance exercise, participants will ingest 32 g protein from whey protein isolate<br>|
| Experimental: Plant-blend isolate<br>Following a bout of bilateral resistance exercise, participants will ingest 32 g protein from a novel plant-blend protein isolate | Behavioral: Bilateral resistance exercise<br>* A bout of bilateral resistance exercise consisting of barbell back squat, leg leg press and leg extension<br>Dietary Supplement: Plant-blend protein<br>* Following execution of bilateral resistance exercise, participants will ingest 32 g protein from plant-blend protein isolate<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Post-exercise myofibrillar protein synthesis following protein ingestion | The fractional synthetic rate of myofibrillar proteins | 4 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Post-exercise myofibrillar protein synthesis during the early postprandial period | The fractional synthetic rate of myofibrillar proteins | 2 hours | 
| Post-exercise myofibrillar protein synthesis during the late postprandial period | The fractional synthetic rate of myofibrillar proteins | 2 hours | 
| Plasma amino acid response | Post-exercise and postprandial change in plasma amino acid concentrations and availability | 4 hours | 
| Serum insulin response | Post-exercise and postprandial change in serum insulin concentrations | 4 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Myofibrillar Protein Synthesis, Resistance Exercise, Plant Protein, Stable Isotopes