The Anabolic and Catabolic Effect of Loaded and Unloaded Exercise on Articular Cartilage Health

Study Overview
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Brief Summary
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The serum concentration of cartilage oligomeric matrix protein (sCOMP) has been considered a mechano-sensitive biomarker of articular cartilage turnover, and N-propeptide of type II collagen (PIIANP) is a proposed biomarker of type II collagen synthesis. Few studies have investigated both the anabolic and turnover response of articular cartilage as a result of acute changes in body weight mass during exercise. Using a repeated measures cross-over design, fifteen healthy adults (age 18-30 years) performed three, 30-minute bouts of treadmill walking exercise under 3 loading conditions: (1) control (no alteration to body mass); (2) loaded (12% increase in body mass using a weighted vest); and (3) unloaded (12% decrease in body mass using lower body positive pressure). Venous blood was collected before, immediately after, and 15 and 30 minutes after exercise to investigate cartilage turnover (sCOMP) and anabolism (PIIANP).

Official Title
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A Preliminary Study on the Effect of Loaded and Unloaded Exercise on Anabolic and Turnover Activity of Articular Cartilage in Healthy Young Adults

Conditions
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Healthy

Intervention / Treatment
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* Other: Control Condition
* Other: +12% Body Mass
* Other: -12% Body Mass


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy 18-30 years old Exclusion Criteria: Traumatic injury to the lower extremity in the past year Hip, knee, or ankle surgery Radiographic evidence of joint degeneration Cardiovascular disease Ankylosing spondylitis Psoriatic arthritis Chronic reactive arthritis Renal problems requiring peritoneal dialysis or hemodialysis Not currently using any prescription or non-prescription medications and/or nutritional supplements

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Control<br>The control condition did 30 minutes of walking exercise with their own body mass. | Other: Control Condition<br>* Control (no alteration to body mass).<br>|
| Experimental: +12% Body Mass<br>The +12% body mass condition did 30 minutes of walking exercise wearing a weighted vest with +12% of their body mass applied. | Other: +12% Body Mass<br>* Loaded walking exercise (12% increase in body mass using a weighted vest).<br>|
| Experimental: -12% Body Mass<br>The -12% body mass condition did 30 minutes of walking exercise using a lower body positive pressure treadmill with -12% of their body mass applied. | Other: -12% Body Mass<br>* Unloaded walking exercise (12% decrease in body mass using lower body positive pressure).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serum cartilage oligomeric matrix protein (sCOMP) | The systemic blood serum concentration of sCOMP was evaluated in all participants under the 3 conditions in a crossover trial. | sCOMP was measured at baseline, immediately post, and 15- and 30-minutes post exercise. | 
| N-propeptide of type II collagen (PIIANP) | The systemic blood serum concentration of PIIANP was evaluated in all participants under the 3 conditions in a crossover trial. | PIIANP was measured at baseline, immediately post, and 15- and 30-minutes post exercise. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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articular cartilage, walking exercise, sCOMP, PIIANP