Ex-vivo Evaluation of the Effect of 2 Probiotic Solutions (Lactibiane ATB and Lactibiane Enfant) and 5 Classes of Drugs on the Intestinal Microbiota

Study Overview
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Brief Summary
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The objective is to test ex-vivo the effects of two probiotic solutions (Lactibiane ATB and Lactibiane Enfant) and five classes of drugs (antibiotic, proton pump inhibitor, non-steroidal anti-inflammatory, anxiolytic and anti-depressant) on microbial microcosms derived from human stool samples (from adults and children) re-cultured under anaerobic conditions.

Official Title
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Ex-vivo Evaluation of the Effect of 2 Probiotic Solutions (Lactibiane ATB and Lactibiane Enfant) and 5 Classes of Drugs on the Intestinal Microbiota

Conditions
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Dysbiosis

Intervention / Treatment
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* Other: Stool sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adult Mother population: Women aged 25-40 years; In good general health, i.e. no chronic pathology and not taking any medication at the time of inclusion and/or long-term treatment, With a regular bowel movement frequency (at least once every 2 days) BMI between 18.5 and 25 Able and willing to participate in the research by complying with the protocol procedures Not objecting to the collection and processing of their personal data Child Daughter population Girl between 1 and 2 years old In good general health, i.e. no chronic pathology and not taking any medication at the time of inclusion and/or on a long-term basis, With a regular stool frequency (at least once every 2 days) Whose mother does not object to the collection and processing of personal data Exclusion Criteria: Adult Mother population : Pregnant or breastfeeding women, Under antibiotic treatment, or having stopped it for less than a month. Under dietary supplementation (prebiotics or probiotics) or having stopped it less than one month before On antidepressants and/or anxiolytics in the month before inclusion Taking a non-steroidal anti-inflammatory drug (NSAID) the month before inclusion. On PPI, or having stopped it less than one month before. Persons under court protection, Person participating in another research study with an ongoing exclusion period, Child Daughter population: Outside the WHO normal growth charts (weight for height) for girls aged 0-2 years (below the 3rd percentile or above the 97th percentile) Undergoing antibiotic treatment, or having stopped it less than a month ago. Having taken a non-steroidal anti-inflammatory drug (NSAID) in the month prior to inclusion. On dietary supplements (prebiotics or probiotics) or having stopped them within the last month Subjects participating in another research study with an ongoing exclusion period

Ages Eligible for Study
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Minimum Age: 1 Year
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Mother<br>Healthy women | Other: Stool sampling<br>* 1 stool sample is taken during the study with a stool sample kit<br>|
| Daughter<br>Healthy daughter from Mother subjects | Other: Stool sampling<br>* 1 stool sample is taken during the study with a stool sample kit<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Transcriptional activity of microbiomes | Transcriptional activity of microbiomes by sequencing of 16S rRNA vs. the gene encoding 16S rRNA | 10 months | 
| Transcriptomic profiles of the communities | Transcriptomic profiles of the communities assessed by meta-transcriptomics | 10 months | 
| Metabolites produced and microbial metabolic pathways | Metabolites produced and microbial metabolic pathways will be assessed by global metabolomics | 10 months |