Investigating Age-dependent Effects of Egg Intake on HDL and Immune Profiles

Study Overview
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Brief Summary
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The goal of this intervention study is to determine whether consumption of different fractions of chicken eggs, including egg whites, egg yolks, and whole eggs, confer different changes in markers of HDL function and T cell profiles in younger vs. older men and women. The study will address the following objectives: Objective 1: Determine if daily consumption of egg fractions differentially alter HDL profiles across age groups. Objective 2: Determine if daily consumption of egg fractions differentially alter immune cell profiles across age groups. Participants will be asked to consume egg whites, egg yolks, and whole eggs on a daily basis for 4-weeks each, and avoid eating eggs for a total of 8 weeks at different points in the study. Participants will additionally be provided guidance on following a generally healthy diet, and will be asked to complete surveys about dietary intake and physical activity, as well as provide blood samples throughout the course of the study. Researchers will compare whether daily consumption of egg whites, egg yolks, and whole eggs differentially alter markers of HDL function and T cell profiles in younger vs. older adults.

Official Title
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Investigating Age-dependent Effects of Egg Intake on HDL and Immune Profiles

Conditions
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Lipoproteins, Inflammatory Response

Intervention / Treatment
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* Other: Whole eggs
* Other: Egg yolks
* Other: Egg whites


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18-30 years old, or 60-70 years old (at time of screening) Body mass index (BMI) < 30 kg/m2 Willing to consume whole eggs, egg whites, or egg yolks on a daily basis during study periods, and refrain from eating eggs during other study periods Do not fit any exclusion criteria Exclusion Criteria: <18 years old; 31-59 years old; >70 years old BMI ≥ 30 kg/m2 or body weight < 110 pounds Weight changes > 10% over the last 4 weeks Self-reported history of diabetes, heart disease, stroke, renal or liver disease, cancer, eating disorders, autoimmune diseases, chronic inflammatory diseases, chronic infections, scleroderma, blood clotting disorders, intravenous drug use, or current pregnancy or lactation Allergy or intolerance to eggs, egg components, or egg products Implanted medical device (e.g., pacemaker) or other health condition that would prevent measurement of body composition by bioelectrical impedance Highly elevated fasting lipid and glucose levels (triglyceride levels higher than 500 mg/dL, fasting glucose higher than 126 mg/dL) Currently taking lipid-lowering medications (e.g. statins, fibrates), anti-inflammatory medications (e.g., NSAIDs, corticosteroids), or medications that primarily affect blood clotting (e.g., warfarin)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Egg whites<br>3 large egg equivalent of liquid egg whites per day for 4 weeks | Other: Egg whites<br>* 3 large egg equivalent of egg whites per day for 4 weeks<br>|
| Experimental: Egg yolks<br>3 large egg equivalent of liquid egg yolks per day for 4 weeks | Other: Egg yolks<br>* 3 large egg equivalent of egg yolks per day for 4 weeks<br>|
| Experimental: Whole eggs<br>3 large whole eggs per day for 4 weeks | Other: Whole eggs<br>* 3 large whole eggs per day for 4 weeks<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in serum concentration of large HDL particles between diet periods | Measurement of serum large HDL particles (nmol/L) at the egg-free run-in period and the end of each intervention arm. | 4 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Lipoproteins, T-Lymphocytes, Eggs, Aging, Diet