High Nucleotide Meal Consumption in Uric Acid and Metabolic Health

Study Overview
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Brief Summary
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The production of protein rich animal products is associated with a large environmental burden, and with ethical considerations. Additionally, the over-consumption of meat might be associated with detrimental effects to health. Using mycoprotein, a food ingredient derived from the fermentation of a fungus, as a meat substitute could result in environmental benefits, and could have a role in managing obesity, diabetes and improving metabolic health. However, naturally produced mycoprotein has a high nucleotide content and little is known about the impact of nucleotides in uric acid levels and other health markers. The aim of this study is to investigate the effect of a meal based on nucleotide rich mycoprotein on uric acid concentrations and other markers of health.

Official Title
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The Acute Effect of a High Nucleotide Mycoprotein Meal in Uric Acid Concentrations and Other Markers of Health

Conditions
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Healthy

Intervention / Treatment
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* Other: Low nucleotide meal
* Other: High nucleotide meal


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI between 18 and 30 Exclusion Criteria: On medication (except contraception) Smokers Diagnosed with any metabolic or cardiovascular conditions BMI < 18 or > 30

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Low nucleotide meal<br>Mixed meal containing mycoprotein with a reduced nucleotide content (  2% nucleotides) | Other: Low nucleotide meal<br>* The participants will consume a mixed meal (sandwich) containing a nucleotide depleted mycoprotein product (low in nucleotides)<br>|
| Experimental: High nucleotide meal<br>Mixed meal containing mycoprotein with a high nucleotide content (  10% nucleotides) | Other: High nucleotide meal<br>* The participants will consume a mixed meal (sandwich) containing a non nucleotide depleted mycoprotein product (high in nucleotides)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in serum uric acid concentrations over a 24 h period |  | Baseline; 15, 30, 45, 60, 90, 120, 150, 180, 210 and 240 minutes after meal; 8, 12 and 24 hours after meal | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood glucose |  | Baseline; 15, 30, 45, 60, 90, 120, 150, 180, 210 and 240 minutes after meal; 24 hours after meal | 
| Serum insulin |  | Baseline; 15, 30, 45, 60, 90, 120, 150, 180, 210 and 240 minutes after meal | 
| Branched chained amino-acids |  | Baseline; 15, 30, 45, 60, 90, 120, 150, 180, 210 and 240 minutes after meal |