Investigation of Supragingival Plaque Microbiome in Early Childhood Caries

Study Overview
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Brief Summary
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The purpose of this study was to characterize the supragingival plaque microbiome in children with early childhood caries and identify potential microorganisms associated with caries.

Detailed Description
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Sixty children with and without early childhood caries were recruited from Trakya University Faculty of Dentistry Department of Pediatric Dentistry. Oral examinations were performed by the same examiner according to diagnostic criteria of World Health Organisation (WHO) and The International Caries Detection and Assessment System (ICDAS) . Mothers' completed a structured questionnaire on the socio-demographic informations, child's feeding practices and oral hygiene habits. After the oral examination, supragingival plaque samples were collected from the posterior tooth surfaces. Supragingival plaque samples were examined with the Illumina MiSeq new generation sequencing method.

Official Title
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Investigation of Supragingival Plaque Microbiome Using Next Generation Sequencing in Early Childhood Caries

Conditions
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Early Childhood Caries

Intervention / Treatment
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* Genetic: Collection of supragingival plaque from caries free children


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 36-71 months old children No history of any medical problem No history of antibiotic in the three months prior to sampling Any sign of early childhood caries, Caries free children Mothers accepted to complete a structured questionnaire Children who cooperate for dental examination and sample collection Exclusion Criteria: Children older than 71 months Medically compromised Children with antibiotic use history at last three months Children refused dental examination and plaque collection Mothers' refused to complete questionnare

Ages Eligible for Study
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Minimum Age: 36 Months
Maximum Age: 71 Months

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Caries-free<br>Caries-free children | Genetic: Collection of supragingival plaque from caries free children<br>* Supragingival plaque samples were collected from posterior molar teeth<br>* Other names: Collection of supragingival plaque from children with early childhood caries;|
| Early childhood caries<br>Children with early childhood caries | Genetic: Collection of supragingival plaque from caries free children<br>* Supragingival plaque samples were collected from posterior molar teeth<br>* Other names: Collection of supragingival plaque from children with early childhood caries;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| V4-16S rRNA and ITS1 rRNA sequencing | Supragingival plaque samples were collected from the tooth surfaces using a sterile periodontal curette. DNA extraction was performed at Sugenomik Biotechnology Laboratory for library preparation and paired-end Illumina MiSeq PE250 sequencing of the V4 region of bacterial 16S rRNA and fungal ITS1 (internal transcribed spacer 1) rRNA genes. | 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Early childhood caries, Next generation sequencing, Oral Microbiome