Effects of Snuff and/or Red Wine om Metabolic Rate

Study Overview
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Brief Summary
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About 14 healthy participants, consume a standardized breakfast combined with either using regular or nicotine-free moist snuff. The metabolic rate is measured every hour for four hours on each occasion starting in the morning. Participants are also randomized to get red wine or non-alcoholic red wine to the meal.

Detailed Description
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About 14 healthy participants, consume a standardized breakfast combined with either using regular or nicotine-free moist snuff. The metabolic rate is measured measured before the meal and then every hour for a total of four hours on each occasion that starts in the morning. Participants are also randomized to get red wine or non-alcoholic red wine to the meal. Since there are four possible combinations of the wine and the snuff, all participants do the test on four occasions. Except for metabolic rate we will also analyze hormone responses regarding appetite or hunger.

Official Title
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Metabolic Effects of Snuff and Red Wine

Conditions
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Metabolic Rate, Hunger, Insulin Sensitivity

Intervention / Treatment
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* Other: Wine with or without alcohol and snuff with or without nicotine


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: No significant disease - Exclusion Criteria: known illnesses, thyroid disease, need to take prednisolone, psychiatric disease,inability to understand Swedish -

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: red wine and snuff<br>regular red wine and moist snuff | Other: Wine with or without alcohol and snuff with or without nicotine<br>* Two by two factorial of wine and snuff with meals<br>|
| Active Comparator: red wine and nicotine-free snuff<br>regular red wine and nicotine-free snuff | Other: Wine with or without alcohol and snuff with or without nicotine<br>* Two by two factorial of wine and snuff with meals<br>|
| Active Comparator: non alcoholic red wine and regular moist snuff<br>non alcoholic red wine and regular moist snuff with nicotine | Other: Wine with or without alcohol and snuff with or without nicotine<br>* Two by two factorial of wine and snuff with meals<br>|
| Placebo Comparator: non-alcoholic red wine and nicotine-free snuff<br>Non-alcoholic red wine and nicotine-free snuff | Other: Wine with or without alcohol and snuff with or without nicotine<br>* Two by two factorial of wine and snuff with meals<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| level of metabolic rate | Measurement of metabolic rate by registration of CO2 in breath and the oxygen uptake | 4 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| sense of appetite | determination of the hormones ghrelin and GLP-1 in serum | 4 hours |