Releasing of Inflammatory Mediators During Retreatment of Root Canals

Study Overview
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Brief Summary
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Nonsurgical root canal retreatment may become essential when the initial endodontic treatment fails because of the persistent intracanal or extracanal infections.The aim of this thesis study is to evaluate the effect of ProTaper Universal Retreatment (PTUR), Reciproc blue and XP-endo Finisher R file systems, which are used in the removal of root canal filling materials during retreatment with different motion kinetics on the release of Substance P, Calcitonin Gene-Related Peptide (CGRP), IL-6 and IL-10 inflammatory mediators in the periapical region

Detailed Description
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Seventy-five individuals with asymptomatic and chronic apical periodontitis teeth with retreatment indication were included in the study. Individuals were randomly divided into three groups according to the file system used to remove root canal filling materials (n = 25): ProTaper Universal Retreatment, Reciproc blue and XP-endo Finisher R.After the removal of the previous root canal filling materials, reshaping and disinfection of the root canals, periapical fluid samples were taken from the root tip of the teeth in all three groups. The amount of Substance P, CGRP, IL-6 and IL-10 mediators that cause pain, swelling and flare-up in periapical fluid samples were measured by ELISA test.

Official Title
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The Effect of Different File Systems Used in Retreatment on the Release of Inflammatory Mediators

Conditions
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Inflammatory Response, Root Canal Infection

Intervention / Treatment
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* Other: Protaper Universal Retreatment
* Other: Reciproc
* Other: XP-Endo finisher R


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients who had single root canal treated teeth with chronic periapical lesion Exclusion Criteria: Patients who are immunocompromised Patients under antibiotic or analgesic medication

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 68 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Retreatment system using in rotational motion<br>Previous root canal filling materials were removed with D1-D2-D3 retreatment files with using an endodontic motor in rotational motion | Other: Protaper Universal Retreatment<br>* D1-D2-D3 Retreatment files were used in 500 rpm and 3 Ncm with X-Smart endomotor<br>|
| Experimental: Retreatment system using in reciprocal motion<br>Previous root canal filling materials were removed with Reciproc 25 file with using an endodontic motor in reciprocal motion | Other: Reciproc<br>* R25 retratment files were used with X-Smart endomotor in reciprocal motion<br>|
| Experimental: Retreatment system using in additional rotational motion<br>Previous root canal filling materials were removed with XP-endo finisher r file with using an endodontic motor in rotational motion | Other: XP-Endo finisher R<br>* XP-Endo finisher r file were used in 1000 rpm 1 Ncm with X-Smart endomotor<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change of inflammatory mediator level | The samples were collected from periapical exudate at the end of the root canal retreatment procedure. The samples were analyzed with ELISA method and change of inflammatory mediator levels were evaluated in pg/ml. | 24 hours |