Evaluation of the Possible Relationship Between Preeclampsia and Periodontal Disease

Study Overview
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Brief Summary
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The primary objective of the study is to assess the incidence and severity of the periodontal infection of patients with preeclampsia. The secondary objective of the study is to analyze the relationship between preeclampsia and periodontal infection, using clinical, biochemical and microbiological methods.

Detailed Description
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Preeclampsia is identified as an important cause for mother and newborn mortality. Inspite of extensive research, the exact etiological relations have not been established. Periodontal disease is a subclinical and persistent infection source which induces the systemic inflammatory reactions and increases the risk of preeclampsia. It is believed that the results will set light to the etiology of preeclampsia and will help to develop strategies to prevent the disease.

Official Title
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Evaluation of the Possible Relationship Between Preeclampsia and Periodontal Disease With Clinical Periodontal, Biochemical and Microbiological Data

Conditions
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Preeclampsia, Periodontal Disease

Intervention / Treatment
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* Diagnostic Test: preeclampsia
* Diagnostic Test: control


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: pregnant women with preeclampsia 37. weeks of pregnant women with no history of preterm labor Exclusion Criteria: Genitourinary infection, pregnancy or pre-pregnancy hypertension, HIV infection, diabetes mellitus, multiple pregnancy, preterm labor / low birth weight, smoker and alcohol use history, IVF pregnancy, placental, cervical and uterine anomalies, pregnancy with intrauterine growth retardation, pregnancies with congenital or chromosomal fetal anomalies, significant vaginal bleeding, infertility stories, drug addicts, poor socioeconomic level

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 34 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| preeclampsia<br>18-40 aged diagnosed with preeclampsia | Diagnostic Test: preeclampsia<br>* liver function tests, kidney function tests and platelets measures the ratio of protein to creatinine<br>* Other names: blood sample, urine test;Diagnostic Test: control<br>* liver function tests, kidney function tests and platelets measures the ratio of protein to creatinine<br>* Other names: blood sample, urine test;|
| control<br>18- 40 aged healthy females over 37. weeks of pregnant with no history of preeclampsia | Diagnostic Test: preeclampsia<br>* liver function tests, kidney function tests and platelets measures the ratio of protein to creatinine<br>* Other names: blood sample, urine test;Diagnostic Test: control<br>* liver function tests, kidney function tests and platelets measures the ratio of protein to creatinine<br>* Other names: blood sample, urine test;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| prostaglandin E2 | prostaglandin E2 levels will be evaulated in salivary, serum and placenta samples using ELISA test Normal value:29-97 pg / ml | 1 Day | 
| TNF-Alpha (Tumor necrosis factor -Alpha) | TNF-Alpha levels will be evaulated in salivary, serum and placenta samples using ELISA test Normal value:42-203pg/ml | 1 day | 
| IL-1beta (Interleukin 1 beta) | IL-1beta levels will be evaulated in salivary, serum and placenta samples using ELISA test Normal value:3-227pg/ml | 1 day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Porphyromonas gingivalis | Presence of Porphyromonas gingivalis will be checked by rPCR method. Score 1: Present Score 2: None Tannerella Forsythia (+,-) | 1 Day | 
| Tannerella Forsythia | Presence of Tannerella Forsythia will be checked by rPCR method. Score:1 Present. Score 2: None | 1 Day |