The Number and Function of Regular T Cell in Patients With Obstructive Sleep Apnea

Study Overview
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Brief Summary
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To compare the number and function of regular T cell of patients with obstructive sleep apnea (OSA) versus that of age-, gender- and BMI (body mass index)-matched controls

Detailed Description
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Patients with obstructive sleep apnea (OSA) experience intermittent hypoxemia and arousal during sleep due to recurrent collapse of upper airway. Epidemiologic evidence showed they were at higher risk for autoimmune diseases. The regular T cell (Treg) has a crucial role in controlling autoimmunity. In this study, 40 OSA patients and 40 age-, gender- and BMI-matched controls will be recruited. Peripheral venous blood of the participants is drawn for numeration of CD4+CD25+FoxP3+ regular T cells by flow cytometry. As well, the CD4+CD25+ regular T cells are enriched by MACS and tested for their suppressive functions on proliferation assays. Th12+ CD4+ cells are also measured.

Official Title
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Impact of Intermittent Hypoxia on the Number and Function of Regular T Cell in Patients With Obstructive Sleep Apnea

Conditions
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Sleep Apnea Syndromes

Intervention / Treatment
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* Biological: OSA


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: OSA patients diagnosed with a polysomnography Agree to participate the study and sign informed consent Exclusion Criteria: Do not agree to participate the study and sign informed consent Has received any treatment or OSA With major comorbidities (such as a diabetes, heart disease, lung diseases, renal diseases, autoimmune diseases, but hypertension excluded) History of sarcoidosis and tuberculosis On treatment of steroid and immunosuppressant

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| OSA<br>The study group enrolled patients with OSA, fulfilling the following requirements 20 60 years ago, female or males Agree participate the study and sign informed consent Has not received any treatment for OSA No obvious comorbidities (including autoimmune diseases) No history of sarcoidosis and tuberculosis No use of steroid and immunosuppressant | Biological: OSA<br>* This study aims to compare the difference in the number and function of regulatory T cells between OSA patients and controls. The investigators will draw peripheral venous blood samples from both groups and measure the number and function of their regulatory T cells. However, some, NOT ALL, of OSA patients may receive continuous positive airway pressure (CPAP) treatment-the standard therapy for severe-to-moderate OSA. Use of CPAP is based on physician's suggestion and patients' decision, but not for this study. The devices are provided by patients themselves and its type/brand is not limited or prespecified. For those with CPAP therapy, their blood sample will be re-collected 26 weeks later to repeat measurement for subgroup analysis.<br>|
| Control<br>The control group enrolled age-, gender- and BMI-matched individuals without OSA, fulfilling the following requirements 20 60 years ago, female or males Agree participate the study and sign informed consent No obvious comorbidities (including autoimmune diseases) No history of sarcoidosis and tuberculosis No use of steroid and immunosuppressant | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The number and function of regular T cells in peripheral venous blood |  | 0-26 week | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The number of Th12+ CD4+ cells |  | 0-26 week | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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sleep apnea, intermittent hypoxia, regulatory T cell, autoimmunity