Chronic Presence of Epstein Barr Virus in Epithelial Cells From Gingiva is Associated With Periodontitis

Study Overview
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Brief Summary
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The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.

Conditions
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Aggressive Periodontitis, Presence / Absence of EBV, Chronic Periodontitis

Intervention / Treatment
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* Procedure: dental root planing
* Procedure: dental root planing
* Procedure: dental root planing


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 10 patients affected by chronic periodontitis 10 patients affected by aggressive periodontitis 10 healthy patients (orthodontics extraction) Subject who have read and understood the information note relative under investigation and who have signed the informed consent major patient Exclusion Criteria: Patient with no contre-indication to periodontal treatment Patient with serious blood disease Patient who exhibits systemic condition incompatible with realization of the study Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants Patient who presents a buccal carcinoma treated with radiotherapy Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy Patient who presents a linguistic or psychic incapacity to understand information

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Health Services Research
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: chronic periodontitis<br> | Procedure: dental root planing<br>* The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus,[1] thus helping to establish a periodontium that is free of disease.[2]<br>|
| Active Comparator: aggressive periodontitis<br> | Procedure: dental root planing<br>* The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus, thus helping to establish a periodontium that is free of disease.<br>|
| Active Comparator: healthy patient (orthodontics extraction)<br> | Procedure: dental root planing<br>* The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus, thus helping to establish a periodontium that is free of disease.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Percentage of gingival epithelial cells infected by EBV (by immunofluorescence and In situ Hybridization) |  | at time=0 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Epithelial tissue infected by EBV (by immunofluorescence and in situ hybridization) |  | at time = 0 | 
| Presence / absence of latency and / or lytic proteins (by immunofluorescence and in situ hybridization) |  | at time = 0 |