Biomarkers in Tissue Samples From Patients Who Have Undergone Neck Dissection for Oral Cancer

Study Overview
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Brief Summary
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RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at tissue samples in predicting oral cancer in patients who have undergone neck dissection for oral cancer.

Detailed Description
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OBJECTIVES: To test preliminary data from node-positive oral squamous cell carcinoma (OSCC) signatures for their ability to predict lymph-positive primaries using a cohort of OSCC tumors. To perform lymph node prediction using sentinel lymph node-biopsied primaries. To validate the preliminary data node-positive OSCC signature and test its ability to predict nodal status. OUTLINE: Patient tissue samples are screened using polymerase chain reaction (PCR) for human papilloma virus-specific primers. Samples are analyzed to identify a nodal-metastasis signature for oral squamous cell carcinoma. Samples also undergo microarray analysis to quantify expression levels for targeted genes. Initial data analysis is performed using Affymetrix® Microarray Suite 5.0 to quantify expression levels for targeted genes.

Official Title
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Molecular Mechanisms of OSCC Tumor Invasion

Conditions
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Head and Neck Cancer

Intervention / Treatment
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* Genetic: microarray analysis
* Genetic: allele-specific oligonucleotide real-time quantitative polymerase chain reaction
* Other: diagnostic laboratory biomarker analysis


Participation Criteria
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Eligibility Criteria
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Have a primary tumor specimen banked by the American College of Surgeons Oncology Group (ACOSOG) as part of clinical trial ACOSOG-Z0360 * All tumor specimens are squamous cell carcinomas of the tongue, floor of the mouth, or gingiva All patients have undergone neck dissections * Pathologically confirmed nodal stage (negative or positive)
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group 1<br>Patient tissue samples are screened using polymerase chain reaction (PCR) for human papilloma virus-specific primers. Samples are analyzed to identify a nodal-metastasis signature for oral squamous cell carcinoma. Samples also undergo microarray analysis to quantify expression levels for targeted genes. Initial data analysis is performed using Affymetrix® Microarray Suite 5.0 to quantify expression levels for targeted genes. | Genetic: microarray analysis<br> <br> * Other names: gene expression profiling;Genetic: allele-specific oligonucleotide real-time quantitative polymerase chain reaction<br> <br> * Other names: ASO RQ-PCR;Other: diagnostic laboratory biomarker analysis<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| overall survival |  | Up to 5 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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stage I squamous cell carcinoma of the lip and oral cavity, stage II squamous cell carcinoma of the lip and oral cavity, tongue cancer