Identify Bacteria Associated With Metabolic Diseases That Reside Both in Intestinal Crypts and in Blood

Study Overview
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Brief Summary
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The investigators will compare 16sRDNA profile in cases and controls in order to identify bacteria associated with metabolic diseases that reside both in intestinal crypts and in blood.

Detailed Description
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Experimental evidence suggests that bacterial translocation from gut to tissue could be an initial step leading to diabetes. To test this hypothesis, a bacterial component that is highly conserved between different species of bacteria, the 16S rDNA gene will be analysed both in intestinal crypt and in blood in patients with type 2 diabetes, obese patients and control.

Official Title
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Identify Bacteria Associated With Metabolic Diseases That Reside Both in Intestinal Crypts and in Blood.

Conditions
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Obesity

Intervention / Treatment
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* Other: obese patients
* Other: diabetic patients
* Other: patients free of obesity and diabetes
* Other: patients free type 2 diabetes


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Between 35 and 75 years old Colonoscopy and biopsies required Diabetes population: Type 2 diabetes Obese population: Android obesity Exclusion Criteria: Inflammatory bowel disease Colonic cancer Bariatric surgery Previous administration of antibiotic (within 15 days) Pregnancy

Ages Eligible for Study
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Minimum Age: 35 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: obese patients<br>Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies | Other: obese patients<br>* collection of blood sampling and biopsies<br>|
| Experimental: Diabetic patients<br>Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies | Other: diabetic patients<br>* collection of blood sampling and biopsies<br>|
| Experimental: Patients free of obesity and diabetes<br>Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies | Other: patients free of obesity and diabetes<br>* collection of blood sampling and biopsies<br>|
| Experimental: patients free type 2 diabetes.<br>Total blood sample, and 4 biopsies : collection of 2 colonic and 2 ileal biopsies | Other: patients free type 2 diabetes<br>* collection of blood sampling and biopsies<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| rDNA comparison between cases and controls | Comparison of 16sRDNA profile in cases and controls in order to identify bacteria associated with metabolic diseases that reside both in intestinal crypts and in blood. | 36 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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gut, bacterial, type 2 diabetes., 16S rDNA gene, intestinal crypt