Clinical and Biochemical Evaluation of Dental and Periodontal Conditions in Children With Juvenile Idiopathic Arthritis

Study Overview
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Brief Summary
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Aimed to be done in the planned thesis to evaluate the dental and periodontal health of patients with newly diagnosed JIA and healthy-periodontal problems with cytokines from saliva and oxidative stress markers non-invasively, and thus, to determine the markers' evaluability in terms of markers in determining the state of inflammation among individuals with and without the disease.

Detailed Description
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Periodontal diseases and inflammatory diseases such as arthritis show similar clinical pathologies such as connective tissue deterioration and bone destruction. They carry the risk of being seen together and the possibility of mutual influence. It is important whether these patients have a difference in cytokine profile from individuals who do not have the disease. In the literature, it has been determined that pro and anti-inflammatory cytokines, which are involved in the pathogenesis of periodontitis, are also involved in the pathogenesis of juvenile idiopathic arthritis (JIA). Cytokines play an undeniable role in the pathogenesis of both diseases. The cytokine profile in periodontal disease is distinctly similar to that of rheumatoid arthritis (RA). As in RA, the level of pro-inflammatory cytokines such as interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) increase in periodontal disease. Again, it is stated that oxidative stress assessment is an important factor in evaluating the development of the disease. Saliva evaluation can be made with new oxidative stress markers that can be used in this sense. On the subject of the proposed thesis; It is to determine the profile of dental and periodontal health in a specific group in terms of biochemical and microbiology using different body fluids together with clinical findings and has an interdisciplinary character. Aimed to be done in the planned thesis; It was aimed to evaluate the dental and periodontal health of patients with newly diagnosed JIA and healthy-periodontal problems with cytokines from saliva and oxidative stress markers non-invasively, and thus, to determine the markers' evaluability in terms of markers in determining the state of inflammation among individuals with and without the disease.

Official Title
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Clinical and Biochemical Evaluation of Dental and Periodontal Conditions in Children With Juvenile Idiopathic Arthritis

Conditions
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Juvenile Idiopathic Arthritis, Gingivitis, Decay, Dental

Intervention / Treatment
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* Diagnostic Test: Humans ELISA kits


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healty patients Exclusion Criteria: used antibiotics within the last 3 months

Ages Eligible for Study
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Minimum Age: 5 Years
Maximum Age: 16 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| patient with juvenile idiopathic arthritis and gingivitis<br>It is a group of patients between the ages of 5 and 16 who have recently been diagnosed with juvenile idiopathic arthritis and gingivitis, have not used medication yet. | Diagnostic Test: Humans ELISA kits<br>* ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.<br>|
| healthy patient group with gingivitis<br>It is a group of healthy patients with gingivitis between the ages of 5-16 who have not taken antibiotics in the last 3 months. | Diagnostic Test: Humans ELISA kits<br>* ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.<br>|
| healthy patient group without gingivitis<br>It is a group of healthy patients without gingivitis between the ages of 5-16 who have not taken antibiotics in the last 3 months. | Diagnostic Test: Humans ELISA kits<br>* ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.<br>|
| patient with juvenile idiopathic arthritis and without gingivitis<br>It is a group of patients between the ages of 5 and 16 who have recently been diagnosed with juvenile idiopathic arthritis and without gingivitis, have not used medication yet. | Diagnostic Test: Humans ELISA kits<br>* ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| biochemical evaluation of saliva in juvenile idiopathic arthritis | Saliva samples were taken from the patients and the levels of interleukin-17, TNF alpha and Total antioxidant status in the saliva were measured with ELISA kits. | baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Evaluation of oral hygiene in patients with juvenile idiopathic arthritis | The number of caries in the patient was evaluated with the DMFT-dft index. | baseline | 
| Evaluation of periodontal health in patients with juvenile idiopathic arthritis | Silness-Loe plaque index (PI) was used to assess periodontal health. | baseline | 
| Examination of periodontal health in patients with juvenile idiopathic arthritis | Gingival index (GI) was also used to examination periodontal health. | baseline | 
| periodontal health in patients with juvenile idiopathic arthritis | Bleeding on prob index were used for periodontal health examination. | baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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pediatric dentistry, periodontal status, juvenile idiopathic arthritis