Infections and Pregnancy Loss: Correlation Between Abortion and Silent Infections.

Study Overview
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Brief Summary
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Correlation between the presence of intracellular viruses/bacteria and the incidence of miscarriage during the first trimester of pregnancy.

Detailed Description
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In this study we will analyse chorionic villi derived from abortion during the first trimester of pregnancy (spontaneous abortion vs voluntary pregnancy interruption), in order to correlate the presence of infective viruses/bacteria and the incidence of miscarriage. The first objective of this project will be to find the viral DNA of HPV, BK and JC polyomaviruses and bacterial DNA of Chlamydia trachomatis, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis in the aborted tissues collected from spontaneous abortions, and to compare the prevalence of these DNAs to those derived from elective specimens. Acronyms: DNA: deoxyribonucleic acid HPV: human papillomavirus PCR: polymerase chain reaction HPyV: human polyomavirus PBMC: peripheral blood mononucleated cell BKV: BK polyomavirus JCV: JC polyomavirus

Official Title
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Silent Intracellular Infections and Early Pregnancy Loss

Conditions
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Pregnancy Loss

Intervention / Treatment
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* Diagnostic Test: Spontaneous miscarriage
* Diagnostic Test: Voluntary pregnancy interruption


Participation Criteria
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Eligibility Criteria
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I.Inclusion and exclusion criteria Inclusion criteria: age of the patients range from 18 to 42 years old; gestational age ranging in the first 12 weeks. Exclusion criteria: medical history or laboratory tests positive for sexually transmitted diseases (HIV, HBV, HCV, LUE) during the last year; Congenital or Acquired Immunodeficiency diseases, or immunosuppressive therapies during the last year; Therapeutic abortion (voluntary interruption of pregnancy, law 194 art 6 comma b) well known causes responsable for spontaneous abortions such as genetic, severe uterine or hormonal abnormalities and use of teratogenic drugs.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 42 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Spontaneous miscarriage<br>The study group will be recruited at admission for uterine cavity revision planned for spontaneous abortion. | Diagnostic Test: Spontaneous miscarriage<br>* Viral DNA and RNA analysis of HPyV and HPV, BKV and JCV. Bacterial DNA and RNA analysis of Chlamydia trachomatis, Ureaplasma parvum, U. urealyticum, Mycoplasma hominis.<br>Diagnostic Test: Voluntary pregnancy interruption<br>* Viral DNA and RNA analysis of HPyV and HPV, BKV and JCV. Bacterial DNA and RNA analysis of Chlamydia trachomatis, Ureaplasma parvum, U. urealyticum, Mycoplasma hominis.<br>|
| Voluntary pregnancy interruption<br>The control group will be recruited at admission for uterine cavity revision planned for voluntary pregnancy interruption. | Diagnostic Test: Spontaneous miscarriage<br>* Viral DNA and RNA analysis of HPyV and HPV, BKV and JCV. Bacterial DNA and RNA analysis of Chlamydia trachomatis, Ureaplasma parvum, U. urealyticum, Mycoplasma hominis.<br>Diagnostic Test: Voluntary pregnancy interruption<br>* Viral DNA and RNA analysis of HPyV and HPV, BKV and JCV. Bacterial DNA and RNA analysis of Chlamydia trachomatis, Ureaplasma parvum, U. urealyticum, Mycoplasma hominis.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Individuation of DNA of intracellular agents (viral und bacterial) in chorionic tissue und blood sample. | The primary outcome of this project is to find the DNA of viral (HPV, HPyV,BK and JC), or bacterial agents (Chlamydia trachomatis, Ureaplasma parvum, U. urealyticum, Mycoplasma hominis) in samples obtained from spontaneous abortion or elective interruption of pregnancy. | December 2014 - April 2017 |