Tissue-specific Effects of Insufficient Sleep

Study Overview
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Brief Summary
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This study plans to learn more about how sleep loss impacts the way the body responds to sugar. This work will have important implications for development of treatments and countermeasures for people who are not able to get enough sleep for various reasons.

Detailed Description
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Participants will stay in the hospital for 6 days and will not be able to leave. Sleep habits will be measured for 7 days before the study. A research diet that is designed to maintain weight will be provided for 3 days before the study and during the study. Multiple tests will be performed, including metabolic testing and muscle and fat biopsies, both when participants first arrive and have had normal sleep and again after 4 nights of only 5 hours time in bed. The visit takes place at the Clinical Translational Research Center (CTRC) at the University of Colorado Denver, Anschutz Medical campus. It will take approximately 2 weeks to complete this study.

Official Title
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Tissue-specific Effects of Insufficient Sleep

Conditions
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Healthy

Intervention / Treatment
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* Behavioral: Insufficient sleep


Participation Criteria
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Eligibility Criteria
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Inclusion criteria: Healthy, lean participants. No regular physical activity (<20 minutes/day). No exercise will be allowed for 3 days prior to study start. Habitual sleep duration between 7-9.25h. Subjects must also be willing and able to keep a 9h time-in-bed schedule for 1 week prior to study admission. Potential subjects must have lived at Denver altitude or higher for at least 3 months to inpatient study. Exclusion Criteria: Current or history of any clinically significant medical, psychiatric, or sleep disorder. Use or history of any drugs, medications, supplements, caffeine, and alcohol. Current or history of shiftwork in six months prior to laboratory study. Travel more than one time zone in three weeks prior to laboratory study. Blood donation in the 30 days prior to inpatient study.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Interventional Model Description: The project is a within-subject consecutive design examining 18 healthy individuals after baseline and after 4 nights of insufficient sleep.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Insufficient sleep<br>Each participant will receive 4 nights of insufficient sleep. | Behavioral: Insufficient sleep<br>* Four days of insufficient sleep<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in insulin sensitivity | Insulin sensitivity measured by hyperinsulinemic euglycemic clamp. | One week | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in macrovascular function | Macrovascular function will be assessed with flow-mediated dilation | One week | 
| Changes in microvascular function | Macrovascular function will be assessed with EndoPAT | One week | 
| Changes in cognitive abilities | Cognitive computer tests performed during study | One week | 
| Changes in circadian rhythms | Salivary dim-light melatonin offset will be assessed | One week | 
| Changes in metabolic tissue function | Tissue biopsies will be performed | One week | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Sleep, Blood Sugar