Microbiome Involvement With Barrett's Esophagus and Progression to Esophageal Adenocarcinoma

Study Overview
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Brief Summary
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This study will examine the hypothesis that altered esophageal microbiome leads to the development of esophageal adenocarcinoma (EAC). The aims of the project are, firstly the confirmation of relational data on the study cohort, using the methods developed and applied at the laboratory at University of Queensland Diamantina Institute (UQDI). Second, to generate a tissue microarray resource future tissue in-situ validation of microbes. And finally, to develop a biobank of clinical isolates of relevant esophageal microbes for future functional studies.

Detailed Description
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Gastroesophageal reflux disease, GERD, can lead to a change of the esophagus lining to a protective cell type, termed Barrett's esophagus (BE). A diagnosis of BE greatly increases the risk of developing esophageal adenocarcinoma (EAC). The incidence of EAC has been rapidly rising in the last 30 years, and the cancer has a high mortality rate. Understanding the involvement of the esophageal microbiota could lead to prevention strategies to this increasing health concern. This study will examine the hypothesis that altered esophageal microbiome leads to the development of esophageal adenocarcinoma (EAC). The aims of the project are, firstly the confirmation of relational data on the study cohort, using the methods developed and applied at the laboratory at University of Queensland Diamantina Institute (UQDI). Second, to generate a tissue microarray resource future tissue in-situ validation of microbes. And finally, to develop a biobank of clinical isolates of relevant esophageal microbes for future functional studies.

Official Title
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Microbiome Involvement With Barrett's Esophagus and Progression to Esophageal Adenocarcinoma

Conditions
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Barrett Esophagus, Esophageal Cancer

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male or females >18 years of age with known or suspected GERD, Barrett's Esophagus and or esophageal adenocarcinoma or from patients who have a history of either condition. Exclusion Criteria: None

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| (GERD) with no Barrett's Esophagus<br>Squamous epithelium from patient with gastroesophageal reflux disease (GERD) with no BE or EAC diagnosed will be collected and a control sample (area with no disease). | |
| Barrett's Esophagus<br>BE/columnar epithelium from patient diagnosed with BE and a control sample (area with no disease). | |
| Cancer Tissue<br>Cancer tissue, and a control squamous epithelium from the same patient. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Microbial community profiling | 16S Ribosomal RNA (rRNA)gene sequencing | 9 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Generate a tissue microarray | After retrieval of blocks, an experienced pathologist will mark the relevant tissue areas to process into microarray | 9 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Microbiome