Uptake and Utilization of Amino Acids by Splanchnic Bed

Study Overview
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Brief Summary
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Splanchnic uptake and metabolism contributes significantly to the differences in flux seen between the intravenous and intragastric ingestion of certain indispensable amino acids. This is the third in a series of studies designed to increase the investigators knowledge of the metabolism of two indispensable amino acids, threonine and lysine, by the splanchnic bed. In the initial two studies the investigators established the neonatal parenteral requirement of threonine and lysine. In the current study, the investigators will be examining the differences in uptake and metabolism of threonine and lysine across the splanchnic bed.

Detailed Description
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We propose that we will see a smaller amount of enterally fed isotopes reach the plasma as compared to the amount in plasma from the parenterally delivered isotopes. We also believe that the amount of enterally delivered threonine isotope that reaches the plasma will be significantly less than the amount of enterally fed lysine isotope.

Official Title
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Determination of the Difference in Uptake and Utilization of Two Amino Acids by the Splanchnic Bed

Conditions
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Healthy Males

Intervention / Treatment
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* Other: Threonine Experimental: Study Period 1
* Other: Lysine Experimental: Study Period 2


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy Males Exclusion Criteria: History of inborn error of metabolism Presence of a chronic disease Have unusual dietary practices Has recently lost weight Receiving pharmacological therapy or hormonal treatment

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Study Period 1<br>AA given by parenteral and enteral routes with tracer 1-13C phenylalanine given to observe differences | Other: Threonine Experimental: Study Period 1<br>* Enteral and parenteral infusion of threonine isotope<br>* Other names: Tracer Isotope - L-[1-13C]threonine;|
| Experimental: Study Period 2<br>AA given by parenteral and enteral routes with tracer 1-13C phenylalanine given to observe differences | Other: Lysine Experimental: Study Period 2<br>* Both intravenous (iv) and intragastric (ig) Lysine isotopes.<br>* Other names: Tracer isotope - L-[1-13C]lysine;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Breath VCO2 |  | -30min, -20min, -10min, +2.5hrs, +3 hrs, +3.5hrs, +4hrs,+4.5hrs, +5hrs | 
| Blood CO2 |  | -30min, -20min, -10min, +2.5hrs, +3 hrs, +3.5hrs, +4hrs,+4.5hrs, +5hrs | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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splanchnic bed, theronine and lysine metabolism uptake, isotopes, metabolism of two indispensable amino acids, threonine and lysine, by the splanchnic bed by Healthy Males