Metabolomic Analysis of Body Fluid of Medulloblastoma in Children

Study Overview
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Brief Summary
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Based on a new mass spectrometry system, body fluid samples were analyzed to verify the role of metabolite analysis in the diagnosis of pediatric medulloblastoma.

Detailed Description
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Screening specific molecular metabolic markers for medulloblastoma after radiotherapy can improve the prediction of clinical diagnosis and prognosis of patients.The purpose of this study is to validate the role of metabolite analysis in the diagnosis of pediatric medulloblastoma based on the analysis of body fluid samples by a new mass spectrometry system.Based on the metabolic markers found by screening, the pathogenic mechanism of metabolic pathway blockade was explained, and the scientific basis for later treatment was put forward.

Official Title
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Metabolomic Analysis of Body Fluid of Medulloblastoma in Children Based on New Mass Spectrometry and Its Application in Clinical Diagnosis and Recurrence Monitoring

Conditions
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Pediatric Medulloblastoma

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The samples are previous biological sample database data; Sample collection time was 2017.05-2019.05; The patient's age is over 3 years old; KPS score ≥50 at the first diagnosis; The patient was a medulloblastoma patient who had received surgical treatment and had not received radiotherapy or chemotherapy before. Exclusion Criteria: Recurrent patients; Patients suffered from other malignant tumors during radiotherapy; The body fluid samples or related clinical data are not perfect; Storage failure of body fluid samples such as lipid dissolution and hemolysis occurred.

Ages Eligible for Study
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Minimum Age: 3 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolic marker(Uracil in Da) | 1. By comparing the expression of small molecule metabolites in body fluids between normal control group and patients with medulloblastoma before radiotherapy, to find specific markers. 2. To evaluate the expression of this metabolic marker in medulloblastoma patients before and after radiotherapy. | Changes of expression profiles of specific metabolic markers before and after radiotherapy,up to 3 months. | 
| Metabolic marker(4-Hydroxyproline in Da) | 1. By comparing the expression of small molecule metabolites in body fluids between normal control group and patients with medulloblastoma before radiotherapy, to find specific markers. 2. To evaluate the expression of this metabolic marker in medulloblastoma patients before and after radiotherapy. | Changes of expression profiles of specific metabolic markers before and after radiotherapy,up to 3 months. | 
| Metabolic marker(Creatine in Da) | 1. By comparing the expression of small molecule metabolites in body fluids between normal control group and patients with medulloblastoma before radiotherapy, to find specific markers. 2. To evaluate the expression of this metabolic marker in medulloblastoma patients before and after radiotherapy. | Changes of expression profiles of specific metabolic markers before and after radiotherapy,up to 3 months. | 
| Metabolic marker(Glutamic acid in Da) | 1. By comparing the expression of small molecule metabolites in body fluids between normal control group and patients with medulloblastoma before radiotherapy, to find specific markers. 2. To evaluate the expression of this metabolic marker in medulloblastoma patients before and after radiotherapy. | Changes of expression profiles of specific metabolic markers before and after radiotherapy,up to 3 months. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Pediatric Medulloblastoma, Metabolomics, Radiation Therapy