Analysis of the Molecular Composition of Tubal Cilia in Patients With or Without Ectopic Pregnancy

Study Overview
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Brief Summary
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Primary Ciliary Dyskinesia associated with abnormalities of lateralization of organs (with existence of a situs inversus in 50% of cases) and secondary fertility disorders related in humans to abnormalities of mobility of sperm but very little data on the structure and function of tubal cilia in women

Detailed Description
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Primary Ciliary Dyskinesia (DCP) is a rare genetic disease (1 in 15,000 individuals at birth), which manifests itself in chronic respiratory infections (bronchopneumopathies and rhinosinusitis type) associated with abnormalities of lateralization of organs (with existence of a situs inversus in 50% of cases) and secondary fertility disorders related in humans to abnormalities of sperm mobility. However, there is very little data in the literature on the structure and function of tubal cilia in women with DCP. Before considering a study of tubal cilia in patients with DCP, it seems necessary to specify in DCP patients or not DCP, the molecular and morphological composition of tubal cilia and to look for ciliary abnormalities in case of GEU

Official Title
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Analysis of the Molecular Composition of Tubal Cilia in Patients With or Without Ectopic Pregnancy

Conditions
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Salpingectomy, Hysterectomy, Primary Ciliary Dyskinesia, Ectopic Pregnancy

Intervention / Treatment
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* Procedure: salpingectomy
* Procedure: hysterectomy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients with scheduled salpingectomy (Ectopic pregnancy, hysterectomy for prolapsus, adenomyosis or myomectomy) Major patients ( >18 years) Exclusion Criteria: Patient not willing to participate in the study

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Salpingectomy for Extra-Uterine Pregnancy<br>patients with salpingectomy for Ectopic Pregnancy | Procedure: salpingectomy<br>* Removal of one of the fallopian tubes.<br>|
| Sham Comparator: hysterectomies<br>patients with hysterectomy for prolapsus, adenomyosis or myomectomy | Procedure: hysterectomy<br>* remove the uterus, whole or only part. It may also involve the removal of the cervix, ovaries, fallopian tubes and other surrounding structures.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| morphological of tubal cilia | Molecular compositor of tubal ciliar by immunofluorescence study of 14 proteins in vivo and in vitro (tissue cells from patient fallopian tubes) | through study completion, an average of 6 week after surgery | 
| Molecular difference in tubal cilia between patients with or without GEU salpingectomy | compares molecular compositor of tubal ciliar by immunofluorecence study of 14 proteins in vivo and in vitro between patients with or without GEU salpingectomy | through study completion, an average of 6 week after surgery | 
| molecular tubal cilia | Ultrastructure morphology of tubal cell cilia by electron microscopy | through study completion, an average of 6 week after surgery | 
| functional composition of tubal cell by video microscopy | amplitude of moment by video microscopy | through study completion, an average of 6 week after surgery | 
| functional composition of tubal cell by video microscopy | frequency of moment by video microscopy | through study completion, an average of 6 week after surgery | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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miscarriage, tubal cilia