Comparison of Nylon Flocked Swabs and Saline Aspirates for Detection Respiratory Viruses

Study Overview
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Brief Summary
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Collection of nasal secretions from infants and toddlers for viral testing is usually done using the nasal washing technique described by Hall in 1975. This is cumbersome. Previous attempts to use swabs have been unsuccessful because the swabs didn't work well. A newly designed swab may work better and in this study we compare the new swab with the old style nasal washing. .

Detailed Description
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Collection of nasal secretions from infants and toddlers for viral testing is typically performed using the nasal saline aspirate technique described by Hall in 1975. Nylon flocked swabs (NFS) and universal transport medium for room temperature (UTM-RT) (Copan Medical, Murrieta, CA) storage media have been found to be an effective collection and transport method for bacteria causing sexually transmitted infections. We adapted these swabs and storage medium to collect respiratory viruses from children less than18 months old and compared detection rates using NFS and traditional nasal aspirates. We will determine the relative roles of the UTM-RT and NFS release and therefore measured viral detection rates of common respiratory pathogens in traditional saline aspirates stored in UTM-RT. Our primary hypothesis is that nasal secretions collection using NFS stored in UTM-RT will lead to a higher detection rate of the respiratory viruses we arestudying; namely RSV, Influenza and human metapneumovirus from than collection of unpreserved saline nasal aspirates in children less than 18 months of age.

Official Title
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Comparison of Nylon Flocked Swabs and Saline Aspirates for Detection Respiratory Viruses

Conditions
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Respiratory Syncytial Virus, Human Metapneumovirus, Influenza, Bronchiolitis, Pediatric

Intervention / Treatment
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* Device: Nylon Flocked swab (Nasal secretion sampling)
* Device: Nylon Flocked swab (Nasal secretion sampling)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Treating clinican ordered RSV antigen testing Exclusion Criteria: Refusal of consent Age > 18 months

Ages Eligible for Study
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Maximum Age: 18 Months

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: 1<br>Nylon Flocked swab Left Nasal Wash right | Device: Nylon Flocked swab (Nasal secretion sampling)<br>* Nasal Aspirate Nylon Flocked swab<br>Device: Nylon Flocked swab (Nasal secretion sampling)<br>* Nylon flocked swab Nasal Wash<br>|
| Experimental: 2<br>Nylon Flocked swab R Nasal Wash L | Device: Nylon Flocked swab (Nasal secretion sampling)<br>* Nasal Aspirate Nylon Flocked swab<br>|
| Experimental: 3<br>Nasal Wash Left Nylon Flocked swab Right | Device: Nylon Flocked swab (Nasal secretion sampling)<br>* Nasal Aspirate Nylon Flocked swab<br>|
| Experimental: 4<br>Nasal Wash R Nylon flocked swab L | Device: Nylon Flocked swab (Nasal secretion sampling)<br>* Nasal Aspirate Nylon Flocked swab<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Viral detection rate by PCR |  | 0 not applicable | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Respiratory Syncytial virus, Bronchiolitis, Pediatric