The Role of Histamine in Muscle Protein Synthesis Following Resistance Training

Study Overview
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Brief Summary
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Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses the following research question: What is the influence of histamine H1 signaling on training adaptations following ten weeks of resistance training in human skeletal muscle. Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.

Official Title
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The Role of Histamine in Muscle Protein Synthesis Following Chronic Resistance Training

Conditions
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Histamine

Intervention / Treatment
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* Drug: Placebo
* Drug: H1 blockade: Telfast: 180mg Fexofenadine
* Behavioral: Resistance training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male 18-45 years Not to medium physically active Exclusion Criteria: Smoking Chronic disease High blood pressure Supplement or medication intake Seasonal allergies Vegetarian / vegan Resistance trained

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: Parallel groups
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: 1: Placebo<br>Lactose | Drug: Placebo<br>* Oral placebo<br>Behavioral: Resistance training<br>* Resistance training: lower body exercise<br>|
| Experimental: H1 blockade: Telfast (180mg Fexofenadine)<br>H1: Telfast (180mg Fexofenadine) | Drug: H1 blockade: Telfast: 180mg Fexofenadine<br>* H1 blockade: oral blockade with 180mg Fexofenadine<br>Behavioral: Resistance training<br>* Resistance training: lower body exercise<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle volume | Change in muscle volume | Before and after the 10 weeks of resistance training | 
| Maximal dynamic strength of leg press, bench press, bicep curl and leg curl | Change in maximal dynamic strength of leg press, bench press, bicep curl and leg curl | Before and after the 10 weeks of resistance training | 
| Muscle fiber hypertrophy in muscle biopsies | Change in muscle fiber hypertrophy in muscle biopsies | Before and after the 10 weeks of resistance training | 
| Matsuda index, to estimate whole body glucose metabolism, will be calculated using an oral glucose tolerance test (OGTT: concentration of glucose and insulin in the blood will be measured before and after drinking 75g glucose) | Change in Matsuda index, to estimate whole body glucose metabolism, will be calculated using an oral glucose tolerance test (OGTT: concentration of glucose and insulin in the blood will be measured before and after drinking 75g glucose) | Before and after the 10 weeks of resistance training | 
| Vascular function measured with a single passive leg movement (sPLM: change in femoral blood flow measured with Doppler ultrasound before and after one passive flexion/extension of the leg) | Change in vascular function with a single passive leg movement (sPLM: change in femoral blood flow measured with Doppler ultrasound before and after one passive flexion/extension of the leg) | Before and after the 10 weeks of resistance training | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood pressure (systolic and diastolic) | Chance in Blood pressure (systolic and diastolic) | Before and after the 10 weeks of resistance training | 
| Resting heart rate | Chance in Resting heart rate | Before and after the 10 weeks of resistance training |