Post-exercise Dietary Protein Strategies

Study Overview
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Brief Summary
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Protein ingestion increases the rate at which the body builds new proteins in skeletal muscle (muscle protein synthesis. This study is designed to examine how the pattern of feeding affects muscle protein synthesis following resistance exercise. There is reason to believe that the large rapid increase in blood amino acid concentrations that accompanies the ingestion of a bolus of protein is important to increasing muscle protein synthesis. Thus, we hypothesize that the consumption a bolus of protein will elevate muscle protein synthesis to a greater extent than the consumption of an equivalent amount of protein that is consumed in small divided doses.

Detailed Description
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The rapid appearance into the blood of essential amino acids, and leucine in particular, may act as an important signal to stimulate muscle protein synthesis after resistance exercise. This may explain why consuming rapidly-absorbed whey protein may have an anabolic edge over slowly-absorbed proteins such as casein. Previous investigations into importance of the rate of absorption to muscle protein synthesis that have used 'fast' and 'slow' proteins have been confounded by differences in amino acid composition. The present study addresses this issue by administering the same protein source, whey, as either a bolus or in small divided 'pulse' doses to achieve divergent amino acid profiles after a bout of resistance exercise. This study is being conducted in young (18-35) men. Our outcome measures include: blood amino acid concentrations, rates of myofibrillar protein synthesis, anabolic intracellular signalling markers

Official Title
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The Effect of the Pattern Post-exercise Aminoacidemia on Myofibrillar Protein Synthesis

Conditions
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Optimal Anabolic Nutrition Interventions

Intervention / Treatment
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* Other: whey protein bolus
* Other: whey protein pulses


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Non-obese men (BMI <27) between the age of 18 and 35 yrs. Exclusion Criteria: Type II diabetes or other known diseases Use of medication Female Other ages or BMI than indicated above Resistance training > 3X/wk

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: protein feeding<br>Participants will complete 2 trials in a cross-over fashion in which they will consume whey protein either as a single bolus or as 10 small divided doses | Other: whey protein bolus<br>* single dose of 25 g whey protein<br>Other: whey protein pulses<br>* 10 2.5 g pulses of whey protein<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Rate of myofibrillar protein synthesis | Rates of myofibrillar protein synthesis will be measured from muscle biopsy samples obtained from subjects participating in the study protocol. | 4 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Myocellular protein phosphorylation | Protein phosphorylation of target proteins will be measured from Western blot analysis of muscle biopsy samples. | 4 months |