Gene Expression Profiles in Chronic Periodontitis

Study Overview
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Brief Summary
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Current concepts of the causes of periodontitis implicate a bacterial infection as the primary cause of the disease. Several bacterial species residing in a biofilm on tooth surfaces are closely associated with periodontitis. Chronic periodontitis (CP) is initiated and sustained by bacterial plaque, but host defense mechanisms play an integral role in its pathogenesis. Gene expression profiling is a powerful means of generating comprehensive genome-level data sets on diseases such as cancer, asthma, rheumatoid disorders and periodontitis, and provides significant insight information for these diseases. Gene expression profiling may provide an evidence for involving genes in the pathogenesis of CP and generate further information other than clinical signs and symptoms of CP. The aim of this study was to identify gene-expression patterns of patients with chronic periodontitis by whole-transcriptome gene-expression analyses.

Official Title
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Gene Expression Profiles in Chronic Periodontitis: A Gene Network-based Microarray Analysis

Conditions
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Chronic Periodontitis

Intervention / Treatment
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* Genetic: Genom-based whole-genome transcriptomic


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: CP patients had at least 20 teeth exhibiting >30% of measured sites with >5mm clinical attachment loss. Had bleeding on probing (BOP) at >50% of the proximal sites. Exclusion Criteria: Had any known systemic diseases that can influence the periodontal status, oral diseases other than chronic periodontitis and ongoing orthodontic therapy. History of hepatitis, HIV infection, or diabetes; requirement for antibiotic prophylaxis, pregnancy or lactation; and long-term use of anti-inflammatory drugs. Should be free of systemic diseases Individuals had to be older than 18 years of age.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients with Chronic Periodontitis<br>All subjects were free of systemic diseases and to be included, individuals had to be older than 18 years of age. Chronic periodontitis was diagnosed according to American Academy of Periodontology 1999 Consensus Report. | Genetic: Genom-based whole-genome transcriptomic<br>* Gene expression profiling is a powerful means of generating comprehensive genome-level data sets.<br>|
| Control Patients<br>Control subjects had no radiographic evidence of bone loss or any sign of past and present periodontitis. All subjects were free of systemic diseases and to be included, individuals had to be older than 18 years of age. | Genetic: Genom-based whole-genome transcriptomic<br>* Gene expression profiling is a powerful means of generating comprehensive genome-level data sets.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| mRNA quantification |  | 2 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Gen networking map |  | 2 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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chronic periodontitis, genetics, microarray analysis, gingiva, genes, inflammation