Resistance Training and Milk Supplementation

Study Overview
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Brief Summary
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Post-resistance training high protein milk supplementation may be a viable method to elicit muscular gains. The investigators hypothesis is that high protein milk supplementation would improve skeletal regulatory markers, hormonal responses and body composition in resistance-trained men.

Detailed Description
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The aim of this investigation was to compare the effects of high protein milk supplementation (daily and after training sessions) during 6 weeks of resistance training on skeletal muscle regulatory markers, hormonal responses and body composition in resistance-trained men. Specific aims of the study are to: To investigate the extent to which high protein milk supplementation will improve anabolic factors, lean body mass, fat mass and decrease catabolic markers after 6 weeks of resistance training in resistance-trained men.

Official Title
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Effects of High-protein Milk Supplementation on Muscular Strength and Power, Body Composition, and Skeletal Muscle Regulatory Markers Following Heavy Resistance Training in Resistance-trained Men

Conditions
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Muscular Hypertrophy

Intervention / Treatment
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* Dietary Supplement: high protein milk
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Aged 18-35 years of age Healthy Males Experienced at resistance training (at least 1 year of prior structured resistance training 3 x week). Exclusion Criteria: smoking chronic alcohol consumption having any medical issues and diseases lactose intolerance the consumption of any dietary supplements (including vitamins or protein supplements) or medications for 6 months prior to participation

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: High protein milk<br>Experimental: High protein milk consumption This arm involved High protein milk whole consumption concomitant with 6 weeks of resistance training. Subject ingested consumed 1 x 250 mL immediately after resistance training and 1 x 250 mL half an hour before bedtime. | Dietary Supplement: high protein milk<br>* This intervention consisted of high protein milk consumption concomitant with 6 weeks of resistance training. Subject ingested consumed 1 x 250 mL immediately after resistance training and 1 x 250 mL half an hour before bedtime.<br>Dietary Supplement: Placebo<br>* This intervention consisted of the consumption of a maltodextrin drink with a 9% solution with a vanilla flavor concomitant with 6 weeks of resistance training. Subject ingested consumed 1 x 250 mL immediately after resistance training and 1 x 250 mL half an hour before bedtime.<br>|
| Placebo Comparator: Placebo<br>This arm involved consumption of a maltodextrin drink with a 9% solution with a vanilla flavor concomitant with 6 weeks of resistance training. Subject ingested consumed 1 x 250 mL immediately after resistance training and 1 x 250 mL half an hour before bedtime. drink r | Dietary Supplement: high protein milk<br>* This intervention consisted of high protein milk consumption concomitant with 6 weeks of resistance training. Subject ingested consumed 1 x 250 mL immediately after resistance training and 1 x 250 mL half an hour before bedtime.<br>Dietary Supplement: Placebo<br>* This intervention consisted of the consumption of a maltodextrin drink with a 9% solution with a vanilla flavor concomitant with 6 weeks of resistance training. Subject ingested consumed 1 x 250 mL immediately after resistance training and 1 x 250 mL half an hour before bedtime.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Circulatory Myokines levels | Fasting blood samples were collected from the antecubital vein using standard procedures and then analyzed to establish concentration of myostatin and follistatin. | 6 weeks | 
| Circulatory hormone levels | Fasting blood samples were collected from the antecubital vein using standard procedures and then analyzed to establish concentrations of testosterone, IGF-1, growth hormone, insulin and cortisol | 6 weeks | 
| Body composition | Lean body mass and body fatness will be evaluated using bioelectrical impedance | 6 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscular Strength | Using a 1 repetition maximum test (defined as the maximal weight an individual can lift for only one repetition) for the bench press and back squat exercises | 6 weeks | 
| Muscular Power | Using the Wingate test | 6 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Hypertrophy, body composition, resistance training, milk