Efficacy of Platelet Rich Plasma (PRP) on Mouth Opening and Pain After Surgical Extraction of Mandibular Third Molars.

Study Overview
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Brief Summary
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Wisdom tooth extraction is the most common surgical procedure being carried out in oral surgical departments. The complications carried by post operative period include pain and trismus and may affect patient's quality of life. PRP is an autologous concentrate of platelet in plasma and accelerate healing by production of growth factors. PRP is prepared from patient's own blood and later packed with gel sponge (Spongistone) in the experimental group immediately after surgical extraction of mandibular third molar teeth, followed by suture placement to close the surgical site.

Detailed Description
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Wisdom tooth extraction is the most common surgical procedure being carried out in oral surgical departments. The complications carried by post operative period includes pain, swelling, trismus , along with disturbance in post extraction wound healing which may significantly affect patient's quality of life. PRP is an autologous concentrate of platelet suspended in plasma and accelerate healing by production of various growth factors. PRP is prepared from patient's own blood and later packed with gel sponge (Spongistone) in the experimental group immediately after surgical extraction of mandibular third molar teeth, followed by suture placement to close the surgical site. PRP speeds up healing by concentration of growth factors which can lessen the inflammation and decreases trismus and pain.

Official Title
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Efficacy of Platelet Rich Plasma (PRP) on Mouth Opening and Pain After Surgical Extraction of Mandibular Third Molars.

Conditions
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Tooth Extraction

Intervention / Treatment
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* Procedure: Platelet Rich Plasma (PRP)
* Procedure: Surgical Extraction


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age between 18-45 iyears Either gender Patients requiring extraction of mandibular 3rd molars ASA grade1 Nonsmokers & non alcoholics Not allergic to any medicines No pain before the extraction procedure No trismus, i.e. normal mouth opening before the extraction procedure - Exclusion Criteria: a) Systemic diseases b) Compromised immune system c) Platelet count less than 1.5 lacs/cmm d) Allergy to drugs e) Patients not willing to participate in the study f) Pregnant females/Lactating mothers g) Presence of pericoronitis, periapical infection or any associated lesion. -

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Control Group<br> | Procedure: Surgical Extraction<br>* Surgical extraction procedure under Local anesthesia on dental chair, followed by spongstone (gel sponge) and suture placement.<br>|
| Experimental: Study Group<br> | Procedure: Platelet Rich Plasma (PRP)<br>* PRP was prepared by withdrawing 8-10 ml of patients own blood (IV from antecubiodal area and collected in a vacuum tube coated with anticoagulant (Na citrate). For activation 1 ml of calcium chloride was added to Platelet Rich Plasma. This layer of approximately 2 ml of PRP was then taken into the residual bone cavity along with gel sponge to stimulate regeneration in wound healing after surgical extraction of mandibular third molar tooth followed by sutures<br>Procedure: Surgical Extraction<br>* Surgical extraction procedure under Local anesthesia on dental chair, followed by spongstone (gel sponge) and suture placement.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Visual Analogue Scale (VAS) | Visual Analogue Scale (VAS) used to quantify pain | 1 day | 
| Visual Analogue Scale (VAS) | Visual Analogue Scale (VAS) used to quantify pain | 3rd post op day | 
| Visual Analogue Scale (VAS) | Visual Analogue Scale (VAS) used to quantify pain | 7th post op day | 
| Trismus | Trismus was quantified/measured using Vernier Calliper. | 1 day | 
| Trismus | Trismus was quantified/measured using Vernier Calliper. | 3rd post op day | 
| Trismus | Trismus was quantified/measured using Vernier Calliper. | 7th post op day |