Cell Free Preimplantation Genetic Testing

Study Overview
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Brief Summary
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This study aims to develop preimplantation genetic testing methods from embryo culture media (cell free). Genetic testing of culture media will be compared to conventional methods based on embryo biopsies.

Detailed Description
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Consenting patients will receive standard clinical IVF and PGT. Culture media from embryos will be collected for analysis. Collection of media will include laser assisted collapse of the blastocyst on day 5, 6, or 7 in order to release blastocoel fluid into the culture media. Embryos diagnosed as aneuploid or monogenic disorder affected (abnormal embryos) will be donated for additional analysis with patient consent. Results obtained from culture media will not be provided to the patients. The information obtained from cf-PGT will be compared to results obtained from embryo biopsies obtained as part of routine PGT and evaluated for concordance and reliability. Abnormal embryos will be rebiopsied ( 3 to 4 times) to evaluate concordance with cf-PGT and the original clinical trophectoderm biopsy PGT results. All cases will be labelled using a 5-digit barcode and embryo number or saliva source code. Because PGT will be conducted under clinical regulatory approval (CAP and CLIA), patient information will be protected under HIPAA guidelines, including sending reports in encrypted secure email per standard operating procedures. All publications and presentations will maintain de-identification of any patient data used. Patients may withdraw from the study at any time by contacting the principal investigator of their respective recruiting site. They will continue to receive standard of care through their treating IVF center. A recruitment target of 200 patients is intended for this study.

Official Title
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Evaluation of Cell Free Preimplantation Genetic Testing

Conditions
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Infertility, Female, Infertility, Male

Intervention / Treatment
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* Other: Spent media collection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients undergoing IVF electing to utilize LifeView testing (PGT) will be offered participation in this study. Exclusion Criteria: None
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients undergoing IVF<br> | Other: Spent media collection<br>* Embryo spent culture media, otherwise discarded as per standard of care, is collected once embryos have completed in vitro culture.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Concordance with PGT | Concordance rates with PGT diagnosis from trophectoderm biopsies | 14 dyas | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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PGT, Noninvasive