Comparison of Plant and Animal Proteins on Amino Acid Concentrations

Study Overview
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Brief Summary
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The study purpose is to assess various protein blends, which differ in protein quality, and the impact on blood amino acid response in male subjects.

Official Title
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Randomized Clinical Trial in Healthy Men Evaluating the Impact of Dairy and Plant-based Protein Blends on Plasma Amino Acids

Conditions
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Healthy Resistance-Trained Males

Intervention / Treatment
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* Other: Animal Proteins (crossover)
* Other: Plant-based protein blends type 1 (crossover)
* Other: Plant-based protein blends type 2 (crossover)
* Other: Plant-based protein blends type 3 (crossover)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy, resistance-trained adult men 18-35 years of age, inclusive, at the screening visit. BMI between 18.5 and 29.9 kg/m² inclusive at sceening Self-reported resistance training experience of no less than two years , and have participated in resistance training of at least 1h two days per week for the past six months. Willing to abstain from any protein supplements for 1 day prior to the study intervention phase (i.e. from the randomised visit, Visit 2) Exclusion Criteria: -

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Animal proteins (crossover)<br> | Other: Animal Proteins (crossover)<br>* Animal proteins mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 1 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 2 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 3 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>|
| Experimental: Plant-based protein blends type 1 (crossover)<br> | Other: Animal Proteins (crossover)<br>* Animal proteins mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 1 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 2 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 3 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>|
| Experimental: Plant-based protein blends type 2 (crossover)<br> | Other: Animal Proteins (crossover)<br>* Animal proteins mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 1 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 2 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 3 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>|
| Experimental: Plant-based protein blends type 3 (crossover)<br> | Other: Animal Proteins (crossover)<br>* Animal proteins mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 1 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 2 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>Other: Plant-based protein blends type 3 (crossover)<br>* Plant-based protein blends mixed with water and consumed as a beverage once by the subject in a 4x4 Williams square crossover design<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma essential amino acids amounts | Following an overnight fast, blood amino acids will be measured over 4 hours | 4 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Kinetic response of the branch chain amino acids | Following an overnight fast, plasma branch chain amino acids amounts (area under the curve) will be measured over 4 hours at fasting | 4 hours | 
| Maximum amount of plasma branch chain amino acid amounts | The maximum plasma branch chain amino acid amount will be measured over 4 hours | 4 hours |