Collecting and Storing Tissue and DNA Samples From Patients Undergoing a Donor Stem Cell Transplant

Study Overview
=================
Brief Summary
-----------------
RATIONALE: Collecting and storing samples of blood, urine, and tissue from patients undergoing a donor stem cell transplant to test in the laboratory may help the study of graft-versus-host disease in the future. PURPOSE: This research study is collecting and storing tissue and DNA samples from patients undergoing a donor stem cell transplant.

Detailed Description
-----------------
OBJECTIVES: To establish a DNA bank from donor and recipient (prior to transplant) peripheral blood for SNP studies. To establish a tissue bank for gene expression studies using donor and recipient (post-transplant) peripheral blood samples. To establish a tissue bank for proteomic studies using donor and recipient blood and urine samples. To establish a tissue bank for various studies using biopsy specimens. To establish a tissue bank for flow cytometric studies using recipient (post-transplant), and sample from stem cell product. OUTLINE: Peripheral blood samples are collected from donors prior to stem cell mobilization and from patients prior to starting preparative regimen. Samples are studied by single nucleotide polymorphism (SNP) analysis, SNP array, proteomic analysis, gene expression, and other immunological laboratory methods. Blood, urine, and tissue samples are preserved for future studies. Extra skin biopsies and gastrointestinal biopsies are performed at diagnosis of graft-vs-host disease and preserved in a tissue bank for flow cytometric studies.

Official Title
-----------------
Tissue Bank for Studies Related to Graft-Versus-Host Disease (GVHD)

Conditions
-----------------
Leukemia, Breast Cancer, Chronic Myeloproliferative Disorders, Gestational Trophoblastic Tumor, Graft Versus Host Disease, Lymphoma, Multiple Myeloma and Plasma Cell Neoplasm, Myelodysplastic Syndromes, Myelodysplastic/Myeloproliferative Neoplasms, Neuroblastoma, Ovarian Cancer, Testicular Germ Cell Tumor

Intervention / Treatment
-----------------
* Genetic: gene expression analysis
* Genetic: microarray analysis
* Genetic: polymorphism analysis
* Genetic: proteomic profiling
* Other: flow cytometry
* Other: immunologic technique
* Other: laboratory biomarker analysis
* Procedure: biopsy


Participation Criteria
=================
Eligibility Criteria
-----------------
DISEASE CHARACTERISTICS: Approved for allogeneic stem cell transplantation Sample of the stem cells from donor must meet 1 of the following criteria: Total peripheral blood stem cell dose > 5 X10^6 CD34+ cells/kg Marrow cell dose > 3 X 10^8 nucleated cells/kg PATIENT CHARACTERISTICS: Platelet count > 50,000/mm³ (for patients undergoing endoscopic biopsies) INR < 1.5 (for patients undergoing endoscopic biopsies) PRIOR CONCURRENT THERAPY: See Disease Characteristics

Ages Eligible for Study
-----------------
Minimum Age: 18 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
No

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Recipients of stem cells with graft versus host disease<br> | Genetic: gene expression analysis<br>* blood collection<br>Genetic: microarray analysis<br>* Blood collection<br>Genetic: polymorphism analysis<br>* Blood collection<br>Genetic: proteomic profiling<br>* Blood collection<br>Other: flow cytometry<br>* Blood collection<br>Other: immunologic technique<br>* Blood collection<br>Other: laboratory biomarker analysis<br>* Blood collection<br>Procedure: biopsy<br>* Blood collection<br>|
| Recipients of stem cells at risk of graft versus host disease<br> | Genetic: gene expression analysis<br>* blood collection<br>Genetic: microarray analysis<br>* Blood collection<br>Genetic: polymorphism analysis<br>* Blood collection<br>Genetic: proteomic profiling<br>* Blood collection<br>Other: flow cytometry<br>* Blood collection<br>Other: immunologic technique<br>* Blood collection<br>Other: laboratory biomarker analysis<br>* Blood collection<br>Procedure: biopsy<br>* Blood collection<br>|
| Donator of stem cells<br> | Genetic: gene expression analysis<br>* blood collection<br>Genetic: microarray analysis<br>* Blood collection<br>Genetic: polymorphism analysis<br>* Blood collection<br>Genetic: proteomic profiling<br>* Blood collection<br>Other: flow cytometry<br>* Blood collection<br>Other: immunologic technique<br>* Blood collection<br>Other: laboratory biomarker analysis<br>* Blood collection<br>Procedure: biopsy<br>* Blood collection<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| DNA bank from donor and recipient (prior to transplant) peripheral blood for single nucleotide polymorphism studies |  | Completion of transplant | 
| Tissue bank for gene expression studies using donor and recipient (post-transplant) peripheral blood samples |  | End of treatment | 
| Tissue bank for proteomic studies using donor and recipient blood and urine samples |  | End of treatment | 
| Tissue bank for flow cytometric studies using recipient (post-transplant), and sample from stem cell product |  | End of treatment |