Telomeres Evaluation in Endometriosis

Study Overview
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Brief Summary
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The purpose of the study is to assess the telomere array of different endometriosis tissue and endometrium from women with endometriosis compared to healthy women. Our hypothesis is that telomere shortening and high telomerase activity will be found in tissues from women with endometriosis.

Detailed Description
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The purpose of the study is to assess the telomere array of different endometriosis tissue and endometrium from women with endometriosis compared to healthy women. Our hypothesis is that telomere shortening and high telomerase activity will be found in tissues from women with endometriosis, compared to women without endometriosis. A prospective study that compares telomeres and telomerase levels in different lesions of endometriosis and endometrial tissue of women with endometriosis and healthy women without endometriosis. The study group will consist of women with suspected endometriosis facing surgical treatment and the control group will consist of healthy women facing gynecologic surgery for different indication. We will sample endometrial tissue in both groups. In the study group we additionally will sample endometriosis lesion. The samples collected will test for histological diagnosis of endometriosis and verification tests will be done to characterize the telomeres array and telomerase levels and other characteristics for genomic instability such as spontaneous aneuploidy. expect telomere shortening and high telomerase activity in endometrial tissue and in endometriosis tissue sample, that won't characterize samples from women without endometriosis.

Official Title
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Telomeres Evaluation in Endometriosis

Conditions
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Endometriosis

Intervention / Treatment
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* Procedure: tissue biopsy during surgical treatment


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: women with suspected endometriosis facing surgical treatment Exclusion Criteria: no endometriosis on histology malignant finding on histology

Ages Eligible for Study
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Minimum Age: 15 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| endometriosis<br>The study group will consist of women with suspected endometriosis facing surgical treatment | Procedure: tissue biopsy during surgical treatment<br>* The samples collected will test for histological diagnosis of endometriosis and verification tests will be done to characterize the telomeres array and telomerase levels and other characteristics for genomic instability such as spontaneous aneuploidy.<br>* Other names: endometrial tissue biopsy;|
| no endometriosis<br>The control group will consist of healthy women facing gynecologic surgery for different indication | Procedure: tissue biopsy during surgical treatment<br>* The samples collected will test for histological diagnosis of endometriosis and verification tests will be done to characterize the telomeres array and telomerase levels and other characteristics for genomic instability such as spontaneous aneuploidy.<br>* Other names: endometrial tissue biopsy;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| characterize the telomeres array and telomerase levels and other characteristics for genomic instability such as spontaneous aneuploidy in endometriosis tissue. |  | the tissue sample will colect at time of surgery | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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endometriosis, telomere, telomerase