Analysis of Dentine Sialophosphoprotein (DSPP) for Root Resorption

Study Overview
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Brief Summary
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This study was conducted after obtaining approval from UKM Research Ethics Committee. Gingival Crevicular Fluid (GCF) from Orthodontic and non-orthodontic patients was collected with the consent of patients to detect Dentine sialophosphoprotein. Spectrometry analysis of Gingival Crevicular Fluid was conducted in collaboration with MIMOS Berhad.

Detailed Description
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Thirty patients were recruited having met the following inclusion criteria of (1) good general health, (2) good periodontal health, (3) caries-free, (4) good oral hygiene. The subjects were divided into 3 groups based on a radiographic assessment on the level of root resorption; normal (no root resorption), mild (less than 2 mm), and severe (more than 2 mm). The GCF samples were taken from the permanent central incisors of untreated subjects in the normal control group (n=14), while in the mild group, samples were collected from the permanent central incisors with mild root resorption (<2 mm) in patients undergoing active orthodontic treatment after 6 months with radiographic evidence (mild group, n=5). Child patients with severe root resorption (>2 mm) were those with primary second molars undergoing physiological root resorption (severe group, n=11). The GCF samples were collected from the mesial and the distal gingival crevicular sulcus of the involved teeth using gingival fluid collection strips (Periopaper, Oraflow, Smithtown, N.Y.). The paper strips were placed within the sulcus (intrasulcular method) approximately 1 to 2 mm into the gingival sulcus and left in situ for 60 seconds. Every sample collection was taken 3 times at the same site with one-minute resting intervals to allow GCF to be replenished into the gingival sulcus.

Official Title
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Dentine Sialophosphoprotein (DSPP) Quantification for Root Resorption Prediction Using Multivariate Versus Univariate Spectrum Analysis

Conditions
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Root Resorption

Intervention / Treatment
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* Biological: DSPP level in GCF


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: good general health, good periodontal health, caries-free, good oral hygiene Exclusion Criteria: Consumption of anti-inflammatory drugs and antibiotics

Ages Eligible for Study
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Minimum Age: 10 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| normal<br>Patients with non-orthodontic treatment and have no root resorption | Biological: DSPP level in GCF<br>* quantification of DSPP<br>* Other names: Root resorption;|
| mild to moderate<br>Patients with orthodontic treatment and have mild to moderate root resorption | Biological: DSPP level in GCF<br>* quantification of DSPP<br>* Other names: Root resorption;|
| severe<br>Child patients with non-orthodontic treatment and have severe root resorption | Biological: DSPP level in GCF<br>* quantification of DSPP<br>* Other names: Root resorption;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| value of wavelength of DSPP in GCF from non-orthodontic patient with no root resorption | The absorbance values at a single wavelength at 450 nm | 1 month | 
| value of wavelength of DSPP in GCF from orthodontic patient with mild to moderate root resorption | The absorbance values at a single wavelength at 450 nm | 1 month | 
| value of wavelength of DSPP in GCF from child patient severe root resorption | The absorbance values at a single wavelength at 450 nm | 1 month | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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DSPP