Shake It Up: Lipidomics of Lipoproteins and Diet

Study Overview
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Brief Summary
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The purpose of this research is to understand how dietary fat is related to cardiovascular disease (CVD). This study tests the effect of different dietary fats on the level of fat in the blood and the particles that transport fat in the blood. The researchers want to know if these particles change after eating meals made with different fats.

Detailed Description
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The overall objective of this work is to discover how the lipidomic composition of postprandial lipoproteins responds to dietary fats of differing fatty acid composition by comparing meals rich in saturated fatty acid (SFA) (from dairy cream) versus polyunsaturated fatty acids (PUFA) (from soybean oil). The information derived from these studies will provide mechanistic insight into postprandial lipid metabolism and will support efforts to identify optimal dietary oil intakes for people.

Official Title
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Shake It Up: Lipidomics of Lipoproteins and Diet

Conditions
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Overweight

Intervention / Treatment
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* Other: Heavy Cream
* Other: Soybean Oil


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Normal to overweight (BMI 18.0-29.9 kg/m^2 Willing to comply with the demands of the experimental protocol Exclusion Criteria: Inability to provide consent Have fasting blood glucose <60 mg/dL or >126 mg/dL Have fasting blood cholesterol >300 mg/dL Have fasting blood triglyceride <100 mg/dL or >300 mg/dL Have uncontrolled hypertension (blood pressure >140/90 mm Hg) Take prescription medications for blood sugar or lipid management or anti-inflammatory steroids (e.g. cortisone) Over the counter use of supplements (fish oil, flax, plant sterol or sterol esters) and unwilling to discontinue use for 6 weeks prior to study initiation Have a history of an eating disorder, unusual dietary pattern (extreme nutrient or food group restriction; erratic meals due to shift work, etc.) Have established cardiovascular, pulmonary, and/or a metabolic disease such as diabetes Have cancer Use tobacco products or nicotine in any form including snuff, pills, and patches, or e-cigarettes/vaping in the past 6 weeks Have alcohol, anabolic steroids, or other substance abuse issues Consume more than 3 alcoholic drinks/week Are lactose intolerant or have an allergy to dairy foods

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Heavy Cream<br>Participants will receive a meal containing 30 g of fat prepared with heavy cream. | Other: Heavy Cream<br>* Participants will receive a meal in the form of a fruit-containing shake containing 30 g of fat prepared with heavy cream.<br>|
| Experimental: Soybean Oil<br>Participants will receive a meal containing 30 g of fat prepared with soybean oil. | Other: Soybean Oil<br>* Participants will receive a meal in the form of a fruit-containing shake containing 30 g of fat prepared with soybean oil.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in lipidomic composition of plasma post-prandial lipoproteins as assessed by incremental area under the curve (iAUC) |  | 0, 1, 2, 4, 6, 8 hours post meal intake | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Normal weight