Leptin and Visfatin in Diabetic Patients With Periodontitis Before and After Periodontal Therapy

Study Overview
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Brief Summary
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Are adipokines involved in pathogenesis of periodontal disease in diabetic patients? Does their level change after periodontal therapy?

Detailed Description
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Patients with chronic periodontitis only and patients with chronic periodontitis and diabetes were evaluated for levels of leptin and visfatin in the gingival tissues before and after non-surgical periodontal therapy.

Official Title
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Effect of Non-surgical Periodontal Therapy on Leptin and Visfatin Expression in Gingival Tissues of Chronic Periodontitis With and Without Type 2 Diabetes Mellitus: A Study Utilizing ELISA and Real Time PCR

Conditions
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Chronic Periodontitis, Type 2 Diabetes Mellitus

Intervention / Treatment
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* Procedure: non-surgical periodontal therapy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: diabetic patients having diabetes mellitus ≥5 years prior to the study. chronic periodontitis with clinical attachment loss > 5 mm. otherwise systemically healthy subjects. Exclusion Criteria: any known systemic diseases other than diabetes. pregnancy or lactation. smokers. receiving periodontal treatment in the last 6 months. taking any drugs or medications other than anti-diabetic drugs.

Ages Eligible for Study
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Minimum Age: 35 Years
Maximum Age: 54 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Intervention Model: Single Group Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: non-surgical therapy<br>non-surgical periodontal therapy with ultrasonic scalers and periodontal curettes | Procedure: non-surgical periodontal therapy<br>* supra and subgingival debridement<br>* Other names: scaling and root planing;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| visfatin levels in gingival tissues | estimation of protein and gene expression of visfatin in patients with chronic periodontitis only and diabetic patients with periodontitis before and after non-surgical periodontal therapy.. | 3 months | 
| leptin levels in gingival tissue | estimation of protein and gene expression of leptin in patients with chronic periodontitis only and diabetic patients with periodontitis before and after non-surgical periodontal therapy.. | 3 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| pocket depth | estimation of pocket depth in patients with chronic periodontitis only and diabetic patients with periodontitis before and after non-surgical periodontal therapy. | 3 months | 
| clinical attachment level | estimation of clinical attachment level in patients with chronic periodontitis only and diabetic patients with periodontitis before and after non-surgical periodontal therapy. | 3 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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leptin, visfatin, chronic periodontitis, diabetes mellitus, non-surgical periodontal therapy