Serum and Semen Vitamin D Level With Semen Parameters

Study Overview
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Brief Summary
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vitamin D may have multiple functions, one of them is modulating reproductive processes and the reproductive role of vitamin D is highlighted by expression of the vitamin D receptors in testis, male reproductive tract and sperms

Detailed Description
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Semen analysis is the corner stone of infertility evaluation as it provides information on the functional status of the seminiferous tubules, epididymis and accessory sex glands. The methods on how the semen should be evaluated are provided by the World Health Organization Vitamin D is synthesized mainly in the skin, where ultraviolet ray B radiation converts 7-dehydrocholesterol to Vitamin D3. Then, Vitamin D3 undergoes two hydroxylation processes first in the liver and second in the kidney Serum 25(OH)D is the major circulating metabolite of vitamin D and reflects vitamin D inputs from cutaneous synthesis and dietary intake. The serum 25(OH)D level is the standard clinical measure of vitamin D status Vitamin D deficiency has been linked to various health disorders including boneheallth, cardiovascular, infectious, oncologic, musculoskeletal, neuropsychologic and reproductive disorders, as well as to overall mortality

Official Title
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Correlation of Serum and Semen Vitamin D Level With Semen Parameters in Males

Conditions
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Vitamin D Deficiency

Intervention / Treatment
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* Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria -males with different semen parameter finding Age: 20-50 years Exclusion Criteria: Varicocele. Genital tract infections. Primary and secondary hypogonadism Hormonal therapy. Calcium and vitamin D supplement. Hepatic or renal failure Diseases or drugs affecting vitamin D absorption -

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| First group: semen parameter showing normospermic<br>group will include 30 male Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA . | Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level<br>* Serum and semen samples will be collected from all participant males and ELISA method used to evaluate the level of vitamin D in serum and semen<br>* Other names: semen analysis;|
| second group: semen parameter asthenozoospermic<br>group will include 30 male Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA . | Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level<br>* Serum and semen samples will be collected from all participant males and ELISA method used to evaluate the level of vitamin D in serum and semen<br>* Other names: semen analysis;|
| Third group: semen parameter oligozoospermic<br>group will include 30 male Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA . | Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level<br>* Serum and semen samples will be collected from all participant males and ELISA method used to evaluate the level of vitamin D in serum and semen<br>* Other names: semen analysis;|
| Forth groupsemen parameter: astheno-teratozoospermic,<br>group will include 30 male Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA . | Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level<br>* Serum and semen samples will be collected from all participant males and ELISA method used to evaluate the level of vitamin D in serum and semen<br>* Other names: semen analysis;|
| Fifth group semen parameter: oligo asthenoteratozoospermia<br>group will include 30 male Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA . | Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level<br>* Serum and semen samples will be collected from all participant males and ELISA method used to evaluate the level of vitamin D in serum and semen<br>* Other names: semen analysis;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| correlation of serum and semen vitamin D level with semen parameters in males | evaluation the level of vitamin D in serum and semen by using ELISA method and correlate it with semen parameters | A year |