The Effect of Various External Pressure on Blood

Study Overview
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Brief Summary
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Rapid infusion of blood is needed occasionally for control massive bleeding. For rapid infusion, external pressure can be used. The investigators will study correlation between external pressure and hemolysis and deformability of red blood cell.

Detailed Description
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Rapid infusion of blood is needed occasionally for control massive bleeding. For rapid infusion, external pressure can be used which cause hemolysis and alteration of red blood cell. The investigators will study correlation between variable external pressure and hemolysis and deformability of red blood cell. Differ from previous study, the investigator will use scanning electron microscope for observation red blood cell.

Official Title
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Comparison of the Effect of Various External Pressure on Blood During Rapid Infusion

Conditions
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Hemorrhage

Intervention / Treatment
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* Device: Auto PC(Acemedical, Goyang-si, Korea) - 0mmHg(control)
* Device: Auto PC(Acemedical, Goyang-si, Korea) - 150mmHg
* Device: Auto PC(Acemedical, Goyang-si, Korea) - 200mmHg
* Device: Auto PC(Acemedical, Goyang-si, Korea) - 250mmHg
* Device: Auto PC(Acemedical, Goyang-si, Korea) - 300mmHg


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy volunteers, without any underlying disease. Exclusion Criteria: history of delivery, abortion in past 6 months. weight : male under 50kg, female under 45kg heart rate under 50 or over 100 fever : over 37.5 blood pressure : over 180/100

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: 5days<br>5 days after blood sampling, various external pressure test will be done. Blood wil be stored in refrigerator(Celsius 4 degree) until study day. External pressure 0, 100, 150, 200, 250, 300mmHg will be applied to each blood sample. | Device: Auto PC(Acemedical, Goyang-si, Korea) - 0mmHg(control)<br>* Blood pass through infusion set without external pressure.<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 150mmHg<br>* Blood pass through infusion set under external pressure (150mmHg)<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 200mmHg<br>* Blood pass through infusion set under external pressure (200mmHg)<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 250mmHg<br>* Blood pass through infusion set under external pressure (250mmHg)<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 300mmHg<br>* Blood pass through infusion set under external pressure (300mmHg)<br>|
| Active Comparator: 35days<br>35 days after blood sampling, various external pressure test will be done. Blood wil be stored in refrigerator(Celsius 4 degree) until study day. External pressure 0, 100, 150, 200, 250, 300mmHg will be applied to each blood sample. | Device: Auto PC(Acemedical, Goyang-si, Korea) - 0mmHg(control)<br>* Blood pass through infusion set without external pressure.<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 150mmHg<br>* Blood pass through infusion set under external pressure (150mmHg)<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 200mmHg<br>* Blood pass through infusion set under external pressure (200mmHg)<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 250mmHg<br>* Blood pass through infusion set under external pressure (250mmHg)<br>Device: Auto PC(Acemedical, Goyang-si, Korea) - 300mmHg<br>* Blood pass through infusion set under external pressure (300mmHg)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Hemolysis and alteration of deformability of red blood cell caused by external pressure |  | 1hour |