Quantitative Analysis of Global Proteome in Bone Samples From Patients With Osteoporotic and Nonosteoporotic Fracture

Study Overview
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Brief Summary
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RATIONALE: Collecting and storing bone samples from patients with osteoporotic and nonosteoporotic fracture to quantitatively analyze the differentially expressed proteins to further explain the relationship between bone iron content and bone mineral density (BMD). PURPOSE: This research study is looking at changes of iron content and differentially expressed proteins in bone samples from patients with osteoporotic and nonosteoporotic. fracture.

Detailed Description
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OBJECTIVES: Establish the iron content in bone specimen in patients with hip fracture. Explore the correlation of BMD and differentially expressed proteins. OUTLINE: Collect human bone specimens from patients with osteoporotic and nonosteoporotic Fracture. Provide a repository for storage of tissue and make these specimens available for approved projects by laboratory-based investigators. Collect clinical data on these patients including bone mineral density. To examine the bone iron content using ICP/MS. Investigate the differentially expressed proteins through quantitative analysing the global proteome of osteoporotic and nonosteoporotic fracture bone specimens.

Official Title
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Collection of Bone Specimens for Quantitative Analysis of Global Proteome and Iron Content Detection

Conditions
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Femoral Neck Fractures

Intervention / Treatment
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* Procedure: total hip arthroplasty


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Ages Eligible for Study: 55-90 Years Genders Eligible for Study: Woman Accepts Healthy Volunteers: NO Study Population: Female patients with hip fracture. Exclusion Criteria: Malignancy or benign ovarian cysts Known chronic or systemic diseases Hormone therapy in the previous 3 months Bone metabolism and drug therapy

Ages Eligible for Study
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Minimum Age: 55 Years
Maximum Age: 90 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: osteoporotic fractures<br>patients with osteoporotic fractures, including femoral neck fracture and pertrochanteric fracture. Total hip arthroplasty or proximal femoral nails was needed. | Procedure: total hip arthroplasty<br>* Bone samples from Hip fracture were collected from the femoral head which has been replaced<br>|
| Sham Comparator: nonosteoporotic fractures<br>patients with nonosteoporotic fractures, including femoral neck fracture and pertrochanteric fracture. Total hip arthroplasty or proximal femoral nails was needed. | Procedure: total hip arthroplasty<br>* Bone samples from Hip fracture were collected from the femoral head which has been replaced<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| the iron content in human bone samples | Bone samples were preprocess and decomposed by microwave.Icp-ms is used for quantitative detection of samples. | 3 month | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The value of bone mineral density | Bone mineral density was measured by double energy X-ray absorptiometer (DXA) in a week before surgery. | one week | 
| Quantitative Analysis of Global Proteome in bone samples | Find the differentially expressed proteins in nonosteoporotic or osteoporotic bone sample through using an integrated approach involving TMT labeling and LC-MS/MS to quantify the dynamic changes of the whole proteome of Human Species. | 5 month |