Assessment of the Relative Increase in Bioavailability of (Non-) Nutrients From a Mixed Salad by Adding Fat Containing Dressing

Study Overview
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Brief Summary
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The hypothesis will be tested that fat can also increase absorption of (non)-nutrients from a salad. Therefore we designed the current study to show an increase in relative absorption (bioavailability) of (non-) nutrients from a mixed salad by adding fat containing dressing.

Official Title
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Assessment of the Relative Increase in Bioavailability of (Non-) Nutrients From a Mixed Salad by Adding Fat Containing Dressing

Conditions
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Healthy Subjects

Intervention / Treatment
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* Other: Test product: Salad with a high dosage fat
* Other: Control product: Salad with low dosage fat


Participation Criteria
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Eligibility Criteria
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Main inclusion Criteria: Body mass index (BMI) ≥ 18.5 and ≤ 30.0 kg/m2. Apparently healthy: no medical conditions, no use of prescribed or over the counter drugs, which might affect study measurements (as judged by study physician) Haemoglobin within normal reference range as judged by the study physician. Total cholesterol, LDL cholesterol, HDL cholesterol and fasting triglycerides within the normal reference range, as judged by the study physician. Main exclusion Criteria: Reported use of any medically- or self-prescribed diet at the moment of screening, or the intention to use any kind of diet in the period between screening and the end of the study. Reported weight loss or gain ≥ 10 % of bodyweight during a period of six months before screening. A known food allergy or intolerance. A dislike to the foods supplied during the study. Use of vitamins or supplements enriched with carotenoid and/or vitamin A and/of vitamin K. Known hypothyroidism or hyperthyroidism.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Test product<br>Test product: Salad with high dosage fat | Other: Test product: Salad with a high dosage fat<br> <br> Other: Control product: Salad with low dosage fat<br> <br> |
| Other: Control product<br>Control product: Salad with low dosage fat | Other: Test product: Salad with a high dosage fat<br> <br> Other: Control product: Salad with low dosage fat<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Area under the curve (AUC in nmol/l*h) of (non) nutrients | Area under the curve (AUC in nmol/l*h) of (non) nutrients. The AUCs are constructed from (non-) nutrient levels measured in plasma chylomicrons at the selected time-points. The size of the areas under the curves will be compared between the test and control dressing. | July 2013 |