Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities

Study Overview
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Brief Summary
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The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.

Official Title
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Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities

Conditions
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Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities, Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile, Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities

Intervention / Treatment
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* Behavioral: Low intensity exercise session
* Behavioral: Moderate intensity exercise session
* Behavioral: High intensity exercise session


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age:18-40 Body Mass Index: 20-30 kg/m2 Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session Women must have regularly occurring menses and must be premenopausal Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository Exclusion Criteria: Evidence/history of cardiovascular or metabolic disease. Taking anti-coagulate medications or medications known to affect metabolism or inflammation Weight instability ≥ ±3kg in the last 6 months Tobacco or e-cigarette users Women must not be pregnant or actively lactating

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: low-intensity continuous exercise<br>subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes | Behavioral: Low intensity exercise session<br>* subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes<br>|
| Experimental: moderate-intensity continuous exercise<br>subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes | Behavioral: Moderate intensity exercise session<br>* subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes<br>|
| Experimental: high-intensity interval exercise<br>subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between | Behavioral: High intensity exercise session<br>* subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| post-translational modifications of skeletal muscle oxidative enzymes | Western blot | 30 minutes before exercise | 
| post-translational modifications of skeletal muscle oxidative enzymes | Western blot | immediately after exercise | 
| post-translational modifications of skeletal muscle oxidative enzymes | Western blot | 60 minutes after exercise | 
| post-translational modifications of Adipose tissue metabolic proteins | Western blot | 15 minutes before exercise | 
| post-translational modifications of Adipose tissue metabolic proteins | Western blot | 75 minutes after exercise | 
| mRNA expression of skeletal muscle oxidative enzymes | qPCR | 30 minutes before exercise | 
| mRNA expression of skeletal muscle oxidative enzymes | qPCR | immediately after exercise | 
| mRNA expression of skeletal muscle oxidative enzymes | qPCR | 60 minutes after exercise | 
| mRNA expression of adipose tissue metabolic enzymes | qPCR | 15 minutes before exercise | 
| mRNA expression of adipose tissue metabolic enzymes | qPCR | 75 minutes after exercise |