Neurocognitive Functions of Recovered COVID-19 Patients

Study Overview
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Brief Summary
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This study is carried on in Wuchang Hospital in Wuhan, China. The investigators plan to recruit 80 patients with COVID-19 and 80 matched healthy control. Using the design of case-control study, the study aims to assess the neurocognitive functions such as executive function and attentional bias in recovered patients with COVID-19 under normal and stress conditions, and to analyze the influencing factors of neurocognitive functions, such as mental health conditions, inflammation indicators and cardiopulmonary function.

Official Title
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An Investigation of Neurocognitive Functions and the Influencing Factors in Recovered Patients With COVID-19

Conditions
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Cognition, Covid-19

Intervention / Treatment
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* Behavioral: Trier Social Stress Test


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Recovered COVID-19 patients or uninfected residents in Wuhan, with an education level above elementary school and are able to complete cognitive tasks; Willing to participate in this study and sign informed consent. Exclusion Criteria: Subjects with body temperature> 38°C; Subjects who are in menstruation and pregnancy; Subjects suffering from cardiopulmonary diseases before the new coronary pneumonia epidemic (such as heart disease, pulmonary hypertension, congestive heart failure, etc.); Subjects with severe disturbances in consciousness, cognitive dysfunction, mental disorders, visual and hearing disorders, bone and joint diseases, etc.; Subjects with abnormal immune system and severe organ dysfunction (heart, liver, kidney, etc.); Subjects who are unwilling to participate in this study and cannot complete all surveys and cognitive function tests.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| COVID-19<br>Recovered COVID-19 patients in Wuhan | Behavioral: Trier Social Stress Test<br>* 8-min oral calculation and 5-min speech<br>|
| Healthy Control<br>Uninfected people in Wuhan | Behavioral: Trier Social Stress Test<br>* 8-min oral calculation and 5-min speech<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| working memory | assessed by the n-back task, including 0-back, 1-back and 2-back | 10 minutes | 
| Cognitive flexibility | assessed by the trail-making task | 3 minutes | 
| Inhibitory control | assessed by the stroop task | 10 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Attention Bias | assessed by the dot-probe task | 15 minutes | 
| Serum TNFalpha | Concentration of serum TNFalpha in ng/ml will be assessed by ELISA | 1 day | 
| Serum IL-6 | Concentration of serum IL-6 in ng/ml will be assessed by ELISA. | 1 day | 
| CRP | hypersensitive C-reactive protein(hs-CRP) in mg/L will be assessed by Turbidimetric inhibition immuno assay. | 1 day | 
| Depression | assessed by Self-Rating Depression Scale, which is consist of 20 items scoring 1 to 4. | 3-5 minutes | 
| Anxiety | assessed by Self-Rating Anxiety Scale, which is consist of 20 items scoring 1 to 4. | 3-5 minutes | 
| PTSD | assessed by PTSD Checklist for DSM-5 (PCL-5) | 3-5 minutes | 
| Sleep | assessed by Pittsburgh sleep quality index (PSQI) | 3-5 minutes | 
| General cognition | assessed by Montreal - Cognitive Assessment (MoCA) | 8-10 minutes | 
| Cardiopulmonary function 1 | maximal inspiration pressure（MIP), maximal expiration pressure（MEP） | 15 minutes | 
| Cardiopulmonary function 2 | Assessed by the 6-minutes' walk test | 10 minutes |