Expression of DMBT1 in Colorectal Cancer Patients

Study Overview
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Brief Summary
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Although colorectal cancer is a preventable and curable disease if early stage tumors are removed, it is still the fourth cause of cancer worldwide and the second leading cause of death in many industrialized countries. The 5-year survival is about 55% often due to a late detection. Then, the identification of sensitive and specific molecular markers is therefore a major challenge for early diagnosis and prognosis of this disease. Preliminary work have reported variations in the expression of DMBT1 (deleted in malignant brain tumor 1), a glycoprotein co-secreted with mucins in the light of the glands, during several stages of colon carcinogenesis. The goal of this study is to study by mass spectrometry (MS), alterations in the repertoire of glycosylation of mucins from colorectal tumors of various stages, grades, and recurrence status.

Official Title
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Modification of the Expression and the Localization of DMBT1 (Deleted in Malignant Brain Tumor 1) in Colorectal Cancer

Conditions
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DMBT1 Protein, Human, Colorectal Cancer

Intervention / Treatment
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* Other: Detection of the expression of DMTB1 by immunohistochemistry


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: for colon cancer: patients surgically treated for colorectal cancer at the Group of hospitals of the Catholic Institute of Lille. for adenomatous polyps : patients treated for adenomatous polyps at the Group of hospitals of the Catholic Institute of Lille. for controls: patients surgically treated for diverticular disease at the Group of hospitals of the Catholic Institute of Lille. Exclusion Criteria: none

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients with colorectal cancer<br>Fresh tissue samples from individuals with colorectal carcinoma taken from the tumor as well as from resection margins will be used for the detection of a potential marker of carcinogenesis. | Other: Detection of the expression of DMTB1 by immunohistochemistry<br> <br> |
| Patients with adenomatous polyps<br>Fresh tissue samples from individuals with adenomatous polyps will be used for the detection of a potential marker of carcinogenesis. | Other: Detection of the expression of DMTB1 by immunohistochemistry<br> <br> |
| Control patients<br>Fresh tissue samples from individuals without colorectal carcinoma will be used as control. | Other: Detection of the expression of DMTB1 by immunohistochemistry<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Semi-quantitative determination of the histological expression of DMBT1 | The expression of DMTB1 will be determined by observation of the slides after immunohistochemistry using an anti-DMBT1 antibody | at baseline | 
| Determination of the histological localisation of DMBT1 | The localisation of DMTB1 will be determined by observation of slides after immunohistochemistry using an anti-DMBT1 antibody | at baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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DMBT1 expression, Colorectal cancer