Beetroot Juice Doses and Anaerobic Performance

Study Overview
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Brief Summary
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The purpose of this study is to examine the effect of varying nitrate doses of beetroot juice supplementation on dynamic, multijoint resistance exercise performance in active, trained individuals. Performance will be assessed based on parameters including neuromuscular efficiency, anaerobic performance capability, oxygen consumption, plasma nitrate/nitrite levels, specific muscle tissue biomarkers including lactate, and anthropometric measurements of select muscle groups.

Official Title
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Evaluation of Various Doses of Beetroot Juice on Anaerobic Performance

Conditions
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Anaerobic Exercise, Dietary Supplementations

Intervention / Treatment
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* Dietary Supplement: Beet It Sport Shot
* Other: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Minimum 6 consecutive months experience in resistance training, engaging at least 2 times per week. Possess sufficient mobility to properly execute movements required in testing protocol Exclusion Criteria: Usage of any banned dietary supplements or performance enhancing drugs (thermogenics, anabolic steroids, prohormones, etc.) Any physical condition or malady that would impede participant from performing exercise or by doing so, endanger the individual

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: High Dosage<br>Subjects will receive approximately 8 mmol of dietary nitrate per day delivered as a commercially available beetroot juice supplement for 7 days. | Dietary Supplement: Beet It Sport Shot<br>* Commercially available concentrated beetroot juice supplement intended for athletes.<br>|
| Active Comparator: Moderate Dosage<br>Subjects will receive approximately 0.5 mmol of dietary nitrate per day delivered as a commercially available beetroot juice supplement for 7 days. | Dietary Supplement: Beet It Sport Shot<br>* Commercially available concentrated beetroot juice supplement intended for athletes.<br>|
| Placebo Comparator: Placebo<br>Subjects will receive a placebo beverage containing negligible nitrate for 7 days. | Other: Placebo<br>* Fruit juice cordial containing negligible nitrate<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle activation and fiber recruitment | Muscle activation/fiber recruitment to be measured in millivolts (mV) using electromyography (EMG) sensor during maximum voluntary contractions (MVC) and compared between study arms. | 5 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma nitrate/nitrite levels | Plasma nitrate and nitrite levels will be measured at baseline and after 7 days of supplementation and compared between study arms. | 5 weeks | 
| Number of repetitions performed | Total number of repetitions in each session and number of repetitions performed at each respective % of 1 repetition maximum during each session will be recorded and compared between study arms. | 5 weeks | 
| Oxygen consumption | Total oxygen consumption during testing sessions will be recorded and compared between study arms. | 5 weeks |