Metabolomic Response to Meal Ingestion

Study Overview
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Brief Summary
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The investigators have recently shown that postprandial sensations correlate with changes in circulating metabolites after a meal ingestion; however this phenomenon was demonstrated with a meal load up to the level of tolerance which involved an unpleasant fullness sensation. The aim of the present study is to determine changes in circulating metabolites of a satiating pleasurable meal. Healthy men (n = 32) will be evaluated after a 5 h fast. Perception measurements and blood samples will be taken before and 20 minutes after ingestion of a palatable probe meal (juice and warm ham and cheese sandwich, total 300 ml, 440 kcal). NMR spectroscopy will be performed to determine plasmatic Low Molecular Weight Metabolites (LMWM) and to characterize lipoprotein profiles (number of particles, lipid content and size of the main three lipoprotein classes (VLDL, LDL and HDL). Homeostatic (satiety, fullness) and hedonic sensations (digestive well-being, mood) will be measured by 10 cm analogical scales.

Official Title
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Factors to Determine the Responses to Meal Ingestion: Circulating Metabolites

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Normal meal


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: non obese Exclusion Criteria: history of gastrointestinal symptoms prior obesity use of medications history of anosmia and ageusia current dieting alcohol abuse psychological disorders eating disorders

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Meal ingestion<br> | Dietary Supplement: Normal meal<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolomic response in serum |  | 30 min | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in digestive well-being measured after the test meal | Change in average well-being measured by 10 score scales at the end of the test meal. | 1 day | 
| Change in satiety measured after the test meal | Change in average satiety measured by 10 score scales at the end of the test meal | 1 day | 
| Change in fullness sensation measured after the test meal | Change in average fullness measured by 10 score scales at the end of the test meal | 1 day | 
| Change in abdominal discomfort/pain sensation measured after the test meal | Change in average abdominal discomfort/pain measured by 10 score scales at the end of the test meal | 1 day | 
| Change in mood measured after the test meal | Change in average mood measured by 10 score scales at the end of the test meal. | 1 day | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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meal ingestion, postprandial sensations, circulating metabolites