n3 PUFA and Muscle-disuse Atrophy in Young Women

Study Overview
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Brief Summary
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This study will examine the influence of n3 PUFA supplementation on the rate of muscle atrophy in women undergoing 2 weeks of unilateral limb immobilization. Assessments in skeletal muscle strength and skeletal muscle volume will also me made before, after and in recovery from immobilization.

Detailed Description
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Biological aging is associated with the loss of skeletal muscle mass and strength resulting in compromised metabolic function and mobility. Throughout life, individuals will also experience periods of reduced physical activity/muscle disuse that independently lower muscle mass and strength accelerating the aging process. The losses in muscle mass with aging and disuse are underpinned by feeding-induced declines in rates of muscle protein synthesis. Thus, strategies to enhance muscle protein synthesis could have clinical implications for those who wish to maintain metabolic health and function during times of muscle disuse. Supplementation with n3 PUFA-enriched fish oil has been shown to potentiate rates of muscle protein synthesis in response to simulated feeding in both younger and older adults. Fish oil supplementation also has been efficacious in enhancing skeletal muscle strength during a period of resistance exercise training. However, no study has examined the impact of fish oil supplementation to enhance muscle protein synthesis and offset declines in muscle mass/strength during a period of immobilization.

Official Title
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Effects of n3 PUFA Supplementation on the Attenuation of Muscle Disuse Atrophy in Young Women

Conditions
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Muscle Atrophy

Intervention / Treatment
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* Dietary Supplement: n3 PUFA-enriched fish oil
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Female Aged 18-30 years Exclusion Criteria: Take any analgesic or anti-inflammatory drugs(s), prescription or non-prescription, chronically will be excluded A history of neuromuscular problems or muscle and/or bone wasting diseases Any acute or chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other metabolic disorders-all ascertained through medical history screening questionnaires Use medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications) Fish allergy

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: n3 PUFA<br>Intervention: n3 PUFA (3000mg of Eicosapentaenoic acid per day and 1800mg of Docosahexaenoic acid per day) | Dietary Supplement: n3 PUFA-enriched fish oil<br>* 3000mg of Eicosapentaenoic acid per day and 1800mg of Docosahexaenoic acid per day<br>|
| Placebo Comparator: Placebo<br>Intervention: Organic Sunflower Oil 5000mg per day | Dietary Supplement: Placebo<br>* Organic Sunflower Oil 5000mg per day<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle Volume | Change in Muscle Volume Measured by Magnetic Resonance Imaging | Baseline (0 days), After (14 days) of unilateral limb immobilization and at 14 days post immobilization | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Integrated Rates of Muscle Protein Synthesis | Change in Muscle Protein Synthesis | Before (0 days), During (0-14 days inclusive), and After (14-28 days inclusive) of unilateral limb immobilization | 
| Skeletal Muscle Strength | Change in Skeletal Muscle Strength | Before (0 days), After (14 days) of unilateral limb immobilization and at 14 days Post immobilization | 
| Muscle Lipid Composition | Change in Muscle Lipid Composition | Baseline (-28 days), and at Before (0 days), After (14 days) of unilateral limb immobilization and at 14 days Post immobilization | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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n3 PUFA