Protein Ingestion Before Training Low

Study Overview
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Brief Summary
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A randomized interventions study using a crossover design. Ten well trained triathletes or bikeriders will be included for to undergo two trail separated by about 3 weeks. The night before the trail each subject will performe a high intense interval trainings session on an ergometer bike. during the night an infusion of aminoacid tracers will be initiated. The subject is woken at 6.30 a.m. Blood samples and a musclebiopsy will be collected prior to the morning bike ride at 8.00a.m. Immediately before the morning bike ride is started the subject will ingest a drink of 0,5g protein or a non-caloric placebo (random order). During the bike ride and the following 4,5 hours blood will be drawn with regular intervals. at time point 0, 60 and 180 min after the training session biopsies are collected.

Official Title
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Nutritional Strategies for Optimizing Adaptations and Recovery Following Training 'Low'.

Conditions
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Protein Metabolism

Intervention / Treatment
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* Other: Protein ingestion
* Other: Non-caloric placebo ingestion


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy well-trained triathletes or cyclists VO2max > 55 ml O2/kg/min Training and has been training more than 7 times a week the last 6 months. Exclusion Criteria: VO2max < 55 ml O2/kg/min Has chronic disease has incjected with tracer on a previous occasion.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Health Services Research
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Protein ingestion<br>Subjects ingested protein drinks | Other: Protein ingestion<br>* A protein drink was ingested prior to the 90min bike ride in one of the completed trails (order randomised).<br>|
| Placebo Comparator: Placebo<br>Subjects ingested a non-caloric placebo drink | Other: Non-caloric placebo ingestion<br>* A placebo drink was ingested prior to the 90min bike ride in one of the completed trails (order randomised).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein fractional synthetic rate (FSR) |  | FSR was measure over at time period of six hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Tracer kinetics dilution model | A-V tracer dilution model was applied across the forearm. | six hours | 
| Muscle signalling | Muscle signalling will be determined using western blotting in each muscle biopsy | Determined in biopsies sampled before and immediatly after 90min exercise in addition to one and four hours after exercise. | 
| Nitrogen excretion | nitrogen excretion is determined by Plasma and urinary carbide analysis as well as plasma NH4+. | Urine is collected during two periods. Collection period one: 12 hours prior to morning exercise. Collection period 2: During and four hours following exercise. Blood samples is drawn repeatedly from before to four hours after exercise. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Dietary protein, Endurance exercise