Association Between Mitochondrial DNA Content and Risk of Acute Leukemia

Study Overview
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Brief Summary
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Compelling epidemiological evidence indicates that alterations of mitochondrial DNA, including mutations and abnormal content of mitochondrial DNA (mtDNA), are associated with the initiation and development of acute lymphoblastic leukemia (ALL).The aim of this study was to explore association between mtDNA content in peripheral blood cells could be used as a risk predictor for ALL.

Official Title
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Association Between Mitochondrial DNA Content and Risk of Acute Leukemia in Young Adult

Conditions
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Lymphoblastic Leukemia in Children

Intervention / Treatment
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* Diagnostic Test: Relative copy number of mitochondrial DNA


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 1.histological confirmed chronic lymphocytic leukemia; 2.preparing for chemotherapy; 3.no preoperative anticancer treatment. Exclusion Criteria: 1.history of other malignancy; 2.blood transfusion within one month or prior bone marrow transplantation; 3.patients who reluctant to sign informed consent.

Ages Eligible for Study
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Maximum Age: 16 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| acute leukemia group<br>150 patients were recruited, who have diagnosed with acute leukemia by bone marrow biopsy | Diagnostic Test: Relative copy number of mitochondrial DNA<br>* Mitochondria are responsible for multiple cellular functions including regulation of energy production, modulation of oxidation-reduction status, generation of reactive oxygen species and apoptosis. Each mitochondrion possesses multiple copies of a mitochondrial genome comprised of independently replicating double stranded DNA (mtDNA).<br>|
| Control group<br>The 50 healthy controls without previous cancer history were recruited from individuals who visited investigator's hospital for physical examination during the same time period as the case enrollment. | Diagnostic Test: Relative copy number of mitochondrial DNA<br>* Mitochondria are responsible for multiple cellular functions including regulation of energy production, modulation of oxidation-reduction status, generation of reactive oxygen species and apoptosis. Each mitochondrion possesses multiple copies of a mitochondrial genome comprised of independently replicating double stranded DNA (mtDNA).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Relative copy number of mitochondrial DNA | The ratio of mitochondrial DNA contents to hemoglobin contents was calculated for each sample from standard curves. After that, the ratio for each sample was normalized to a calibrator DNA in order to standardize between different runs, and then defined as the measurement of relative mtDNA contents.Relative expression of mtDNA were measured in young adults suffered from chronic lymphocytic leukemiawhen compared with control group. | From date of admission until the date of discharging from hospital, assessed up to 5 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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mitochondrial DNA