Effects of Coffee on Hepatic Steatosis Induced by a High Fructose Diet

Study Overview
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Brief Summary
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This study will assess whether coffee consumption protects against fructose-induced hepatic steatosis in healthy humans whether the protective effect of coffee is dependent on it's antioxidant composition

Detailed Description
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Epidemiological studies suggest that coffee consumption improves glucose homeostasis in insulin resistant subjects. An increase in intrahepatic lipids (hepatic steatosis) is highly prevalent in patients with the metabolic syndrome and may be used as a marker of altered hepatic lipid metabolism. Such an increased hepatic lipids content can be experimentally produced in healthy humans by a 6-day high fructose diet. The purpose of this study is to evaluate whether coffee prevents hepatic lipid deposition in healthy male subjects fed a fructose-rich hypercaloric diet. Both caffeine and antioxidants (yet unspecified) may be involved.. To sort out the role of caffeine and antioxidants, we will test 3 different soluble coffee, ie fully torrefied decaffeinated coffee , partially torrefied decaffeinated coffee, and partially torrefied caffeinated coffee.

Official Title
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Effects of Coffees With Various Compositions of Antioxidants on Hepatic Steatosis Induced by a High Fructose, Hypercaloric Diet

Conditions
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Hepatic Steatosis

Intervention / Treatment
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* Dietary Supplement: Ctl
* Dietary Supplement: High fructose diet; no coffee
* Dietary Supplement: fully torrefied, caffeine-free coffee
* Dietary Supplement: partially torrefied, caffeine-free coffee
* Dietary Supplement: Partially torrefied, caffeinated coffee


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI between 19 and 15 kg/m2 less than 30 min physical activity /day habitual coffee consumption less than three cupy /day consumption of caffeine-containing sodas less than 2 servings/day non-smoker Exclusion Criteria: consumption of alcohol more than 40g/day presence of metallic foreign bodies history of eye surgery family history of diabetes mellitus history of food intolerance vegetarians

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Factorial Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Ctl<br>control isocaloric diet; no coffee | Dietary Supplement: Ctl<br>* Control, isocaloric diet; no coffee<br>|
| Placebo Comparator: HF<br>Hypercaloric. high fructose diet; no coffee | Dietary Supplement: High fructose diet; no coffee<br>* Hypercaloric, high fructose diet; no coffee<br>|
| Experimental: C1<br>Hypercaloric, high fructose diet; caffeine-free, torrefied coffee | Dietary Supplement: fully torrefied, caffeine-free coffee<br>* Hypercaloric, high fructose diet + coffee<br>|
| Experimental: C2<br>Hypercaloric, high fructose diet; caffeine-free, partially torrefied coffee | Dietary Supplement: partially torrefied, caffeine-free coffee<br>* Hypercaloric, high fructose diet + coffee<br>|
| Experimental: C3<br>Hypercaloric, high fructose diet; caffeinated, partially torrefied coffee | Dietary Supplement: Partially torrefied, caffeinated coffee<br>* Hypercaloric, high fructose diet + coffee<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| intra-hepatocellular lipid (IHCL) concentration |  | will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| fasting plasma triglycerides |  | will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement | 
| fasting net lipid oxidation |  | will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement | 
| fasting net carbohydrate oxidation |  | will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement | 
| whole body ketone bodies turnover and oxidation (13C 3-hydroxybutyrate) |  | will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement | 
| whole body glucose turnover (6,6 2H2 glucose) |  | will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement | 
| whole body glycerol turnover (2H5 glycerol) |  | will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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coffee, hepatic steatosis, fructose, lipids