Study of Skin Parameters in the PROOF Cohort

Study Overview
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Brief Summary
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This study aims to evaluate skin aging of an homogeneous population of 77 years old patient selected among the PROOF cohort who has been continuously investigated by cardiological examination. Skin aging will be explored by macroscopic and microscopic evaluation. In particular, skin aging will be measured by an index of clinical aging (SCINEXA) and other complementary measures such as facial photos, standard and High Definition dermoscopy, elastography, sebumetry, in vivo and ex vivo confocal microscopy, and classical histological examinations.

Official Title
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Study of Skin Parameters in the PROOF Cohort

Conditions
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Skin Aging

Intervention / Treatment
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* Other: Dermatological clinical examination
* Other: Biopsy
* Other: Confocal microscopy in vivo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: included in the PROOF cohort Form consent signed Exclusion Criteria: Past history of surgery and / or treatment of aesthetic medicine neurological disorders insulin-dependent diabetes, corticosteroids or retinoids (isotretinoin, ...) > 1 year

Ages Eligible for Study
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Minimum Age: 75 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| PROOF<br>Healthy inhabitants of the city of Saint-Etienne, France, and aged 65 years at the inclusion date. (NCT00759304) | Other: Dermatological clinical examination<br>* Clinical pictures, Dermatoscopy, Elastography, Sebumetry<br>Other: Biopsy<br>* Skin biopsy for examinations by confocal microscopy ex vivo and histopathology<br>Other: Confocal microscopy in vivo<br>* Examination by confocal microscopy in vivo<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| SCINEXA score | The aging of the skin is evaluated by a semi-quantitative composite of 19 clinical variables : the score SCINEXA | Day 1 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| in vivo confocal microscopy | Skin aging evaluated by in vivo confocal microscopy | Day 1 | 
| ex vivo confocal microscopy | Skin aging evaluated by ex vivo confocal microscopy ( thickness of the epidermis with and without the stratum corneum, dermis thickness , number of sweat glands per unit volume). | Day 1 | 
| histology | Skin aging evaluated by conventional histology (thickness of the epidermis and dermis, presence and degree of elastosis , flattening of the dermo- epidermal junction) | Day 1 | 
| RAMAN | The composition of subcutaneous fat measured by ex vivo RAMAN spectroscopy | Day 1 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Skin Surface Microscopy, Skin Aging, PROOF