Pilot Study Characterizing Adipose Tissue Leukocytes by Flow Cytometry/Microscopy in Lean, Obese and Psoriatic Subjects

Study Overview
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Brief Summary
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Obesity is an insulin resistance-associated metabolic disorder which is a hallmark of and risk factor for type 2 diabetes and the metabolic syndrome, often linked to cardiovascular disease, certain cancers and inflammatory diseases.The phenotyping of subcutaneous adipose tissue (SAT) hematopoetic cells from obese subjects by flow cytometry, microscopy and gene expression will enable us to identify inflammation in this tissue and may help us to understand the causes and consequences of obesity in order to determine how these cells might be implicated in the initiation and/or progression of the aforementioned diseases.

Detailed Description
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Obesity is an insulin resistance-associated metabolic disorder which is a hallmark of and risk factor for type 2 diabetes and the metabolic syndrome. It is also often linked to cardiovascular disease, certain cancers (e.g., colorectal, esophageal, endometrial, and ovarian), and inflammatory diseases (e.g., psoriasis, osteoarthritis,inflammatory bowel disease).The phenotyping of subcutaneous adipose tissue (SAT) hematopoetic cells from obese subjects by flow cytometry, microscopy and gene expression will enable us to identify inflammation in this tissue and may help us to understand the causes and consequences of obesity in order to determine how these cells might be implicated in the initiation and/or progression of the aforementioned diseases.

Official Title
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A Pilot Study to Characterize Adipose Tissue Leukocytes by Flow Cytometry and Microscopy in Lean, Obese and Psoriatic Subjects (Lean/Obese)

Conditions
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Psoriasis, Obesity

Intervention / Treatment
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* Procedure: Adipose tissue biopsy (fat biopsy)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Psoriatic lean cohort (BMI between 18-24.9 Kg/m2): moderate to severe plaque-type, at least 5% of BSA Psoriatic obese cohort (BMI between 30-40 Kg/m2): moderate to severe plaque-type, at least 5% of BSA Lean/non-psoriatic cohort: BMI between 18-24.9 Kg/m2 Overweight/obese non-psoriatic cohort: BMI between 30-40 Kg/m2 Subjects must be 18-65 years of age Exclusion Criteria: Having received any systemic treatment for psoriasis within the last 30 days history of bleeding disorder weight loss of 10 pounds in the last four weeks current smoker Known diagnosis of any unrelated autoimmune disease or inflammatory disease ( i.e. lupus, atopic dermatitis, rheumatoid arthritis). Currently taking NSAIDS, aspirin, (if > once a week, stopped <30 days ago). Aspirin 81mg may be permitted if the Framingham Risk Score is < 10 Having received any anti-inflammatory medication within the last 30 days Current use of any anti-coagulants LFTs > 2 x upper normal limits HIV infection Pregnant Less than 6 weeks post partum history of cardiovascular disease (MI, CHF, CVA) Any cancer diagnosis within the last 5 years Symptoms of acute illness such as upper respiratory infection, bronchitis, gastroenteritis, or fever within 3 days of Visit 1 Any medical, psychological or social condition that, in the opinion of the Investigator, would jeopardize the health or well-being of the participant during any study procedures or the integrity of the data ingestion of DHA or fish oil within last 90 days hypertension as defined as > 140 systolic and > 90 diastolic after 10 minutes of resting on 2 antihypertensives Use of statins within the last 30 days

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| lean (BMI≤ 24.9 Kg/m2)<br>Adipose tissue biopsy (fat biopsy) in 9 lean (BMI≤ 24.9 Kg/m2) | Procedure: Adipose tissue biopsy (fat biopsy)<br>* An adipose tissue biopsy will be performed after a 10-12 hour overnight fast. A small sample of fat tissue will be removed from the subject for gene expression analysis. A subcutaneous fat biopsy ( 3 ml) will be obtained from the lower abdomen following local anesthesia.<br>|
| obese (BMI= 30-40 Kg/m2)<br>9 obese (BMI= 30-40 Kg/m2) | Procedure: Adipose tissue biopsy (fat biopsy)<br>* An adipose tissue biopsy will be performed after a 10-12 hour overnight fast. A small sample of fat tissue will be removed from the subject for gene expression analysis. A subcutaneous fat biopsy ( 3 ml) will be obtained from the lower abdomen following local anesthesia.<br>|
| psoriatic lean<br>9 psoriatic lean | Procedure: Adipose tissue biopsy (fat biopsy)<br>* An adipose tissue biopsy will be performed after a 10-12 hour overnight fast. A small sample of fat tissue will be removed from the subject for gene expression analysis. A subcutaneous fat biopsy ( 3 ml) will be obtained from the lower abdomen following local anesthesia.<br>|
| psoriatic obese<br>9 psoriatic obese | Procedure: Adipose tissue biopsy (fat biopsy)<br>* An adipose tissue biopsy will be performed after a 10-12 hour overnight fast. A small sample of fat tissue will be removed from the subject for gene expression analysis. A subcutaneous fat biopsy ( 3 ml) will be obtained from the lower abdomen following local anesthesia.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Biomarkers for phenotyping a new type of leukocyte | Biomarkers for phenotyping a new type of leukocyte | 5 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Psoriasis, Obesity