The Different Response of Endocrine Hormones to Hypoglycemia in Patients With Type 1 or Type 2 Diabetes

Study Overview
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Brief Summary
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Endocrine hormones, not only blood glucose responsive hormones, but also sexual hormones, thyroid hormones, growth hormones and so on, may have response and activites in hypoglycemia. However, the different responses of endocrine hormones to hypoglycemia in patients With diabetes remain unclear.

Detailed Description
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This study will observe the changes of insulin, C-peptide, glucagon, growth hormone, IGF-1, ACTH, cortisol, thyroid function, sexual hormone in newly diagnosed men with type 2 and type 1 diabetes mellitus using hyperinsulinaemic hypoglycaemic clamp technique

Official Title
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A Study of Endocrine Hormone Changes in Newly Diagnosed Patients With Type 2 and Type 1 Diabetes Using Hyperinsulinaemic Hypoglycaemic Clamp Technique

Conditions
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Diabetes

Intervention / Treatment
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* Diagnostic Test: The hyperinsulinaemic hypoglycaemic clamp


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Patients newly diagnosed male patients with type 1 or type 2 diabetes (diagnostic criteria of World Health Organization) aged 18-50 years has not been treated with any hypoglycemic drugs HbA1c>9% Normal subjects: healthy and had no known illness Exclusion Criteria: patients with insulin allergy. any history of hypoglycemic agent intake or were presently taking hypoglycemic agents any abnormality in liver or kidney function on blood and urine investigations any history of systemic corticosteroid use in 3 months any recent infections or acute medical events any other obvious conditions or associated diseases determined by the researcher: such as severe cardiopulmonary diseases, endocrine diseases, neurological diseases, tumors and other diseases, other pancreatic diseases, history of mental diseases.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Newly diagnosed male obese patients with type 2 diabetes<br> | Diagnostic Test: The hyperinsulinaemic hypoglycaemic clamp<br>* Glucose will be clamped at 5.0 mmol/L for approximately 30 minutes, and then be lowered to 3.0 mmol/L for 30 minutes<br>|
| Newly diagnosed male non-obese patients with type 2 diabetes<br> | Diagnostic Test: The hyperinsulinaemic hypoglycaemic clamp<br>* Glucose will be clamped at 5.0 mmol/L for approximately 30 minutes, and then be lowered to 3.0 mmol/L for 30 minutes<br>|
| Newly diagnosed male patients with type 1 diabetes<br> | Diagnostic Test: The hyperinsulinaemic hypoglycaemic clamp<br>* Glucose will be clamped at 5.0 mmol/L for approximately 30 minutes, and then be lowered to 3.0 mmol/L for 30 minutes<br>|
| Healthy, non-obese men<br> | Diagnostic Test: The hyperinsulinaemic hypoglycaemic clamp<br>* Glucose will be clamped at 5.0 mmol/L for approximately 30 minutes, and then be lowered to 3.0 mmol/L for 30 minutes<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| endocrine hormone changes | changes of endocrine hormones during hyperinsulinaemic hypoglycaemic clamps | 20 minutes |