Evaluation of the Acute Effect of Water-Pipe Smoking on the Respiratory System

Study Overview
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Brief Summary
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Title: The acute effect of water pipe smoking on exhaled nitric oxide (eNO) and exhaled breath condensate (EBC) pulmonary function tests in healthy volunteers Objectives: To evaluate the acute effect of one cession of water pipe smoking on airway inflammation as assessed by exhaled nitric oxide (eNO) and exhaled breath condensate (EBC) in healthy volunteers. Design: Prospective study evaluating these parameters before and after 30 minutes of water pipe smoking . The changes in inflammatory parameters pre and post smoking will be evaluated blindly. Sample size: 100 participants Participant selection: Adults subjects who regularly smoke water pipe . Intervention: Each subject will undergo evaluation including a respiratory questionnaire , pulmonary function tests , exhaled nitric oxide (eNO) and exhaled breath condensate (EBC) and carboxy- hemoglobin levels . All measurements will be evaluated before and after one cession of 30 minutes water pipe smoking Primary outcome parameter: Change in carboxy- hemoglobin Secondary outcome parameter:Change in peripheral eosinophils count, pulmonary function tests, change in FeNO, and in inflammatory parameters in EBC before and after water pipe smoking

Conditions
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Carboxyhemoglobin, Vital Signs, Fractional Exhaled NO, Exhaled Breath Condensate

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age > 18 Y.O. Have smoked Water-pipe at least once before. Exclusion Criteria: Breast feeding Women. S/P Bacterial Or Viral infection, Last 2 weeks P.O. Steroids Water-pipe smoking, last 24 hours Cigarette smoking, last 6 hours Intensive exposure to smoke (fire), last 24 hours S/P Intensive Care Hospitalization, last 3 months

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| carboxy- hemoglobin | blood test | before and immediatly after 30 min of smoking | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Eosinophils count | blood test | before and immediatly after 30 min of smoking | 
| heart rate | heart beats count | before and immediatly after 30 min of smoking | 
| Respiratory rate | Respiratory rate | before and immediatly after 30 min of smoking | 
| Blood pressure | Non Invasive Blood pressure mesaurement | before and immediatly after 30 min of smoking | 
| Spirometry | Pulmonary function test | before and immediatly after 30 min of smoking | 
| Visual Analog Score 1-10 (1=bad 10=good) | Questionnaire | before and immediatly after 30 min of smoking | 
| Exhaled breath Condensate | R-tube tests | before and immediatly after 30 min of smoking | 
| Fractional Exhaled NO | Exhaled NO test | before and immediatly after 30 min of smoking | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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water pipe smoking acute effect