The Effect of Non-surgical Periodontal Therapy on the Gingival Crevicular Fluid Levels of Prolactin Hormone in Patients With Periodontitis.

Study Overview
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Brief Summary
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The present study will be conducted on fifty participants. All participants will receive a full-mouth clinical examination, and the following periodontal parameters will be recorded: Plaque Index (PI). Gingival Index (GI), probing depth (PD), and clinical attachment level (CAL). All of these measurements will be recorded at six sites at baseline for all participants and 3 months after non-surgical periodontal therapy for the study group. GCF samples will be collected from the buccal aspects of teeth that had the highest signs of inflammation and attachment loss for periodontitis group at baseline and 3 months after non-surgical periodontal therapy. As for controls, the samples will be collected from buccal aspect of upper first molar at baseline. The samples will be assayed for prolactin by using an enzyme linked immunosorbent assay kits (Elisa) according to the manufacturer instruction.

Official Title
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The Effect of Non-surgical Periodontal Therapy on the Gingival Crevicular Fluid Levels of Prolactin Hormone in Patients With Periodontitis.

Conditions
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Periodontitis, Prolactin in GCF

Intervention / Treatment
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* Procedure: non-surgical periodontal therapy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subjects with moderate to severe chronic periodontitis, with probing pocket depth (PPD) ≥ 4-6 mm and clinical attachment level ≥ 4-5 mm, according to the American Academy of Periodontology.7 Highly motivated and cooperative patients Exclusion Criteria: The presence of any systemic disease. Taking medications for the last 3 months before conducting this study. Pregnancy and lactation. Any type of previous periodontal treatment (surgical or non-surgical) in the preceding 6 months. Smoking (former or current).

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: periodontitis group<br>in this group, prolactin in GCF will be assessed at baseline and after 3 months of receiving non-surgical periodontal treatment | Procedure: non-surgical periodontal therapy<br>* scaling and root planing<br>|
| No Intervention: control group<br>in this group of systemically and periodontally healthy participants, prolactin in GCF will be assessed at baseline only | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| prolactin in GCF | prolactin level in GCF by ELISA | 3 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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periodontitis, Prolactin, GCF, non-surgical periodontal treatment