Study of the Physiology of Pre-eclampsia and Vascular IUGR With Constitution of a Biological Collection

Study Overview
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Brief Summary
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Preeclampsia and intrauterine growth retardation (IUGR) are serious and frequent pathologies, specific to pregnancy. They represent 70 000 new cases a year, or 9% of pregnancies and cause 50,000 premature births per year in France. The consequences in terms of morbidity and perinatal morbidity and the medical and economic costs make it an issue public health. Pre-eclampsia associates maternal hypertension with dysfunction kidney. There is no cure for pre-eclampsia or IUGR vascular during pregnancy. These pathologies invariably evolve towards a maternal and / or fetal aggravation sometimes very fast. Primary prevention and secondary education and screening for these pathologies are still insufficient. A better understanding of the pathophysiology of these placental vascular pathologies is necessary for the development of supported medical, obstetric and pediatric that will improve the state of health maternal and neonatal

Official Title
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Study of the Physiology of Pre-eclampsia and Vascular Intrauterine Growth Restriction With Constitution of a Biological Collection

Conditions
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Pre-Eclampsia

Intervention / Treatment
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* Other: collect of placenta, blood and urinary samples at delivery


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: pregnant women With or without vascular pathology Exclusion Criteria: refusal to participate multiple pregnancy major fetal malformation diagnosed during pregnancy follow-up

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Vascular group<br>Pregnant women with vascular pathology | Other: collect of placenta, blood and urinary samples at delivery<br>* collect of placenta, blood and urinary samples at delivery<br>|
| Control group<br>Pregnancy without any vascular complication Delivery before or after 37 weeks of gestation (GW) In case of delivery after 37GW: birth by cesarean delivery | Other: collect of placenta, blood and urinary samples at delivery<br>* collect of placenta, blood and urinary samples at delivery<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| genomic analysis on placenta | by chromatin immunoprecipitation-sequencing (ChIP-seq) | day of delivery | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| genomic analysis on blood | by ChIP-seq | day of delivery | 
| genomic analysis on placenta | by immunohistochemistry an polymerase chain-reaction | day of delivery | 
| VEGF blood level |  | day of delivery | 
| PLGF blood level |  | day of delivery | 
| sFlt1 blood level |  | day of delivery | 
| sKDR blood level |  | day of delivery |