Exploring Muscle Breakdown During Exercise Recovery

Study Overview
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Brief Summary
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This study will allow researchers to explore how muscle responds to heavy exercise. The researchers will characterise rates of muscle protein breakdown and synthesis 24hours after heavy exercise with a post exercise protein polyphenol or placebo supplementation. This will inform strategies to help people recover from heavy exercise.

Detailed Description
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It is well known that resistance exercise increases rates of muscle protein synthesis and breakdown, with the rise in synthesis greater in magnitude and duration. The time course of rates of muscle protein synthesis increase dramatically 24-72h following eccentric exercise, even in a non-exercised control leg. This data is consistent with a much larger increase in muscle protein breakdown than previously thought. However, the response of muscle protein breakdown following damaging eccentric exercise, and its relationship with muscle protein synthesis, has not yet been elucidated. Moreover, it is unclear whether a protein and polyphenol nutritional intervention influence rates of muscle protein breakdown to accelerate recovery. This study will allow researchers to test the hypothesis that eccentric exercise increases rates of muscle protein breakdown independently of muscle contraction per se. If this hypothesis is supported, it will highlight that recovery strategies from muscle damage should also target supporting muscle protein breakdown.

Official Title
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Exploring Muscle Breakdown During Exercise Recovery

Conditions
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Muscle Damage, Healthy

Intervention / Treatment
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* Dietary Supplement: Recover Vegan Protein Supplement
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Recreationally active 18-40 years of age Exclusion Criteria: Regular and structured involvement in resistance training Inactive participants Habitual dietary protein intake <0.8g/kg bw/day and >2.0g/kg bw/day Regular use of nutritional supplements known to suppress inflammation or modulate protein metabolism Any diagnosed metabolic conditions (diabetes), cardiovascular disease or hypertension Current musculoskeletal injury Chronic use of anti-inflammatory medicines Any known disorders in muscle metabolism Individuals with an allergy to egg, fish, wheat or soy ingredients as the product is manufactured in a facility that contains these ingredients Allergy to lidocaine Having received or ingested a stable isotope tracer containing 15N in the past A personal or family history of epilepsy, seizures or schizophrenia Stomach bleeding or stomach ulcer Kidney failure Liver problems, such as fibrosis, cirrhosis or failure Crohns disease or ulcerative colitis Chickenpox or shingles Pregnant or trying to get pregnant Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension. Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes) Recent (within the last 6 months) or current musculoskeletal injury (leg fracture)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Vegan Protein<br> | Dietary Supplement: Recover Vegan Protein Supplement<br>* Post exercise protein supplement (26g pea protein)<br>|
| Placebo Comparator: Placebo<br> | Dietary Supplement: Placebo<br>* Post exercise placebo supplement (32g maltodextrin)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein breakdown rate | Muscle protein breakdown rate (FBR, measured in %/h) 24hours post eccentric exercise. | 1hour | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein synthesis rate | Muscle protein synthesis rate (FSR, measured in %/h) 24hours post eccentric exercise. | 3hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Skeletal muscle, Postabsorptive, Muscle protein breakdown, Muscle protein synthesis