Genomic Maps of Endometrial Tissues of Patients With Endometriosis and Healthy Controls

Study Overview
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Brief Summary
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Endometriosis is a chronic inflammatory disease that affects 10-17% of women in childbearing age, 35-60% of women with chronic pelvic pain and can cause infertility, costing as much as 2.8 billion dollars per year. For this reason, every step taken in diagnosis and treatment is of great importance both materially and spiritually. Patients can be diagnosed about 8-9 years after the onset of their complaints due to inadequacy of diagnostic methods In the study, it is decided to look at the basis of the disease, endometrial cells. And aimed to find the difference between the two women who are thought to be no different from each other among our current knowledge but one has endometriosis while other have not.

Detailed Description
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Endometriosis is the appearance of a tissue similar to the endometrium in the ovaries, large intestine, and many other organ walls, except the uterus. The reason is unknown yet. Severe periodic pain, infertility, and pain during intercourse, affects approximately 2 million women in Turkey on average, 10% of women around the world. Endometriosis is a chronic inflammatory disease that affects 10-17% of women in childbearing age, 35-60% of women with chronic pelvic pain and can cause infertility, costing as much as 2.8 billion dollars per year. For this reason, every step taken in diagnosis and treatment is of great importance both materially and spiritually. Patients can be diagnosed about 8-9 years after the onset of their complaints due to the inadequacy of diagnostic methods In the study, it is decided to look at the basis of the disease, endometrial cells. And aimed to find the difference between the two women who are thought to be no different from each other among our current knowledge but one has endometriosis while others have not. For this reason, mapping of all genomes with the nanoclonal technique, the most advanced genetic screening method currently available from endometrial samples of patients and healthy women, is to identify the possible copy numbers, translocation, insertion and inversion anomalies, and to name them functionally by proteomic analysis. We believe that the results obtained from our study will guide the diagnostic tests and possible gene therapy in the future.

Official Title
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Comparison of Genomic Maps of Endometrial Tissues of Patients With Endometriosis and Healthy Controls Via Nanochannel Technique and Proteomic-genomic Analyzes.

Conditions
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Endometriosis, Endometrioma

Intervention / Treatment
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* Diagnostic Test: genetic testing with nanoclonal technique


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: surgically diagnosed with endometriosis disease no diagnosıs for malignancy gave informed consent for application to the study enrolle as patient group females between 18-45 years old without any health conditions applied to the clinic for routine checkups for control group Exclusion Criteria: being diagnosed with any kind of malignancy having trouble with endometrial cavity sampling

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| control<br>does not have endometriosis or related diseases and not under any drug therapy does not have any benign or malign disease | Diagnostic Test: genetic testing with nanoclonal technique<br>* endometrial sampling<br>|
| patient<br>has laparoscopically proven endometriosis | Diagnostic Test: genetic testing with nanoclonal technique<br>* endometrial sampling<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| diagnostic testing | finding a genetic difference which specifically preserved in endometriosis patients but not in healthy controls. To analyse the different region on chromosomes and name the pathophysiology underlying it | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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genetic analysis, endometriosis, nanoclonal, copy number