Kisspeptin Levels in Early Pregnancy

Study Overview
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Brief Summary
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Kisspeptins are a family of neuropeptides that are critical for the puberty initiation and female fertility. The investigators aimed to investigate in this study kisspeptin levels in early pregnancy, ectopic pregnancy, and early pregnancy loss.

Detailed Description
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Clinicians can easily do a differential diagnosis of early pregnancy, miscarriage, and ectopic pregnancy when the human chorionic gonadotropin (hCG) values higher than 2000 mIU/mL with transvaginal ultrasound. However, in cases where the hCG value is below the critical threshold of 1500 to 2000 mIU/mL (often called the discrimination level) and ultrasound is not likely to be diagnostic, the pregnancy is of unknown location. Plasma or serum kisspeptin is mainly derived from the placenta during pregnancy and plasma kisspeptin levels significantly increase across pregnancy. Plasma kisspeptin levels could be used as a potential biomarker for the discrimination of miscarriage and ectopic pregnancy. All statistics will be carried out with the use of SPSS (ver. 21, IBM). A P value of <.05 is considered to be statistically significant. Pearson chi-square test will be used to evaluate between-group differences by pregnancy outcome for categoric data. Kisspeptin differences will be calculated between groups with one way ANOVA.

Official Title
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Can Kisspeptin be Used for Differential Diagnosis of Early Pregnancies?

Conditions
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Early Pregnancy, Ectopic Pregnancy

Intervention / Treatment
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* Diagnostic Test: blood test


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Women aged between 18-37 years presented with between 5-6 weeks of gestation by last menstrual period. Exclusion Criteria: Women older than 37 years or younger than 18 years Women with a molar pregnancy, multiple gestations, gestational age past 6 weeks at the time of blood draw, or if pregnancies were assisted conceptions

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 37 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group 1<br>Ectopic pregnancy | Diagnostic Test: blood test<br>* blood test (serum samples) for biochemical examination<br>|
| Group 2<br>Early viable pregnancy | Diagnostic Test: blood test<br>* blood test (serum samples) for biochemical examination<br>|
| Group 3<br>incomplete miscarriage | Diagnostic Test: blood test<br>* blood test (serum samples) for biochemical examination<br>|
| Group 4<br>Healthy women | Diagnostic Test: blood test<br>* blood test (serum samples) for biochemical examination<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Kisspeptin levels in early pregnancy | Kisspeptin levels can be used for discrimination between ectopic pregnancy and incomplete miscarriage. | January 2019-January 2020 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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kisspeptin, ectopic pregnancy