The Metabolic Effects of Breaks in Sedentary Time

Study Overview
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Brief Summary
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The purpose of this study is to determine whether acute bouts of sedentary behaviour (with or without breaks and/or structured physical activity) result in measurable changes in metabolic health in children and youth.

Detailed Description
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North American children spend the majority of their waking hours engaging in sedentary behavior (e.g. sitting). Research in adults and animal models suggests that even short bouts of uninterrupted siting may result in acute changes increases in cardiometabolic risk, although to date no studies have investigated this relationship in children and youth. The purpose of this study is to assess the feasibility of a protocol which will expose participants aged 10-14 years to a full day of uninterrupted sitting, and to determine the impact of this protocol on markers of cardiometabolic risk (insulin, glucose, triglyceride, HDL-C and LDL-C) as well as subsequent food intake and physical activity. The impact of uninterrupted sitting will be compared to the impact of interrupted sitting, with and without the inclusion of moderate physical activity.

Official Title
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The Metabolic Effects of Breaks in Sedentary Time

Conditions
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Insulin, Feasibility, Glucose, Lipids, Eating

Intervention / Treatment
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* Other: Sedentary
* Other: Sedentary With Breaks
* Other: Sedentary With Breaks and Physical Activity


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 10-14 Normal weight or obese Exclusion Criteria: Overweight Overt diabetes or heart disease (or taking medications to treat this conditions) Being unambulatory or otherwise unable to perform the study protocol

Ages Eligible for Study
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Minimum Age: 10 Years
Maximum Age: 14 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Sedentary<br>In this condition, participants will engage in 8 hours of uninterrupted sedentary behaviour. | Other: Sedentary<br>* In this condition, participants will engage in 8 hours of uninterrupted sedentary behaviour.<br>|
| Experimental: Sedentary With Breaks<br>In this condition participants will engage in 8 hours of sitting, although the sitting will be interrupted every 20 minutes. During these interruptions participants will spend 2 minutes walking at an intensity equivalent to 30% of VO2peak. | Other: Sedentary With Breaks<br>* In this condition participants will engage in 8 hours of sitting, although the sitting will be interrupted every 20 minutes. During these interruptions participants will spend 2 minutes walking at an intensity equivalent to 30% of VO2peak.<br>|
| Experimental: Sedentary With Breaks and Physical Activity<br>In this condition participants will engage in 8 hours of sitting, although the sitting will be interrupted every 20 minutes. During these interruptions participants will spend 2 minutes walking at an intensity equivalent to 30% of VO2peak. Participants will also engage in 20 minutes of structured physical activity at an intensity of 60% of VO2peak in both the morning and afternoon. | Other: Sedentary With Breaks and Physical Activity<br>* In this condition participants will engage in 8 hours of sitting, although the sitting will be interrupted every 20 minutes. During these interruptions participants will spend 2 minutes walking at an intensity equivalent to 30% of VO2peak. Participants will also engage in 20 minutes of structured physical activity at an intensity of 60% of VO2peak in both the morning and afternoon.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in Insulin Net Incremental Area-Under-The-Curve (iAUC) | Insulin levels will be assessed multiple times throughout the day, and the resulting values will be used to calculate AUC. | Baseline, 1.5, 3, 4.5, 6, and 7.5 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in Triglyceride Net Incremental Area-Under-The-Curve (iAUC) | Blood draws will be taken several times throughout the day, and the resulting values will be used to calculate AUC. | Baseline, 1.5, 3, 4.5, 6, and 7.5 hours | 
| Change in HDL-Cholesterol (HDL-C) Net Incremental Area-Under-The-Curve (iAUC) | Blood draws will occur throughout the day, and the resulting values will be used to calculate AUC. | Baseline, 1.5, 3, 4.5, 6, and 7.5 hours | 
| Change in LDL-Cholesterol (LDL-C) Net Incremental Area-Under-The-Curve (iAUC) | Blood draws will occur throughout the day, and the resulting values will be used to calculate AUC. | Baseline, 1.5, 3, 4.5, 6, and 7.5 hours | 
| Change in Glucose Net Incremental Area-Under-The-Curve (iAUC) | Blood draws will occur throughout the day, and the resulting values will be used to calculate AUC. | Baseline, 1.5, 3, 4.5, 6, and 7.5 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Sedentary Behavior, Cardiometabolic Risk, Pediatrics