Glycemic Response of Co-ingesting Carbohydrate and Amino Acids

Study Overview
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Brief Summary
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The study will investigate if consumption of amino acid mixtures results a change in glycemic response of carbohydrate (white rice) and insulinemic response at different doses and timing (before consumption of carbohydrate).

Detailed Description
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Healthy male subjects will be served with amino acid mixtures in combinations of two doses and three time points along with white rice as control. The combinations were either low or high levels of amino acid mixtures. This was consumed together or 15 min, 30 min before consumption of white rice. Postprandial blood glucose and plasma insulin concentrations were measured at fasting and every 15 min after consumption of treatment meal until 60 min after the consumption of white rice. Subsequent blood sample were taken at 30 min interval until 210min. The glucose and insulin in the blood will be analysed.

Official Title
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Glycemic Response of Co-ingesting Carbohydrate and Amino Acids

Conditions
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Metabolic Diseases

Intervention / Treatment
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* Other: Rice and Amino Acid mixtures


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male Chinese ethnicity Age between 21-50 years Body mass index (BMI) between 18-24.9 kg/m2 Waist circumference ≤ 90 cm for men Normal blood pressure (<120/80 mmHg) Fasting blood glucose < 6 mmol/L Exclusion Criteria: dislike the consumption of Essence of Chicken participating in competitive sports allergic to amino acids and rice metabolic diseases (such as diabetes, hypertension and the metabolic syndromes) Glucose-6-Phosphate-Dehydrogenase (G6PD) Deficiency on prescription medication smokers

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: White rice control<br>Subjects will consume white rice as control (50g carbohydrate) | Other: Rice and Amino Acid mixtures<br>* Rice and amino acid mixture<br>|
| Experimental: co-ingest low dose of amino acid<br>Subjects will receive 68 ml of amino acid mixture together with white rice | Other: Rice and Amino Acid mixtures<br>* Rice and amino acid mixture<br>|
| Experimental: co-ingest high dose of amino acid<br>Subjects will receive 136 ml of amino acid mixture together with white rice | Other: Rice and Amino Acid mixtures<br>* Rice and amino acid mixture<br>|
| Experimental: pre-load low dose15 min<br>Subjects will receive 68 ml of amino acid mixture 15 min before consumption of white rice | Other: Rice and Amino Acid mixtures<br>* Rice and amino acid mixture<br>|
| Experimental: pre-load low dose 30 min<br>Subjects will receive 68 ml of amino acid mixture 30 min before consumption of white rice | Other: Rice and Amino Acid mixtures<br>* Rice and amino acid mixture<br>|
| Experimental: pre-load high dose 15 min<br>Subjects will receive 136 ml of amino acid mixture 15 min before consumption of white rice | Other: Rice and Amino Acid mixtures<br>* Rice and amino acid mixture<br>|
| Experimental: pre-load high dose 30 min<br>Subjects will receive 136 ml of amino acid mixture 30 min before consumption of white rice | Other: Rice and Amino Acid mixtures<br>* Rice and amino acid mixture<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glycemic response | Blood glucose will be assessed via finger prick during fasting and every 15 minutes for the first 60 min after the consumption white rice and subsequently every 30 min interval until 210 min | 210 minutes post consumption of carbohydrate | 
| Insulinemic response | Plasma insulin will be assessed via finger prick during fasting and every 15 minutes for the first 60 min after the consumption white rice and subsequently every 30 min interval until 210 min | 210 minutes post consumption of carbohydrate |