Alcohol Exposure and Airway Hyperresponsiveness

Study Overview
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Brief Summary
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Alcohol has consequences including increased risk for upper respiratory tract infections, pneumonia, acute respiratory distress syndrome (ARDS), and alcohol-induced asthma. The investigators have established that airways are specifically impacted by alcohol exposure because the airways are heavily exposed to the vapor phase of alcohol during drinking. These preliminary studies demonstrate that brief alcohol administration significantly attenuates airway hyperresponsiveness (AHR) in a mouse model leading to the hypothesis that alcohol exposure modifies airway hyperresponsiveness through a cAMP/NO- dependent mechanism.

Detailed Description
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Alcohol has well-established consequences in the lung including increased risk for upper respiratory tract infections, pneumonia and acute respiratory distress syndrome (ARDS). There have even been a few reports of alcohol-induced asthma. Data from the investigators' laboratory have established that the airways are specifically impacted by alcohol exposure. Because the airways are heavily exposed to the vapor phase of alcohol during drinking and airway motor tone is modulated by cAMP, the investigators speculated that airway bronchial motor function would be altered in mice fed alcohol. The investigators' preliminary studies demonstrate that brief alcohol administration significantly attenuates airway hyperresponsiveness (AHR) in a mouse model. This novel finding has led us to hypothesize that: Alcohol exposure modifies airway hyperresponsiveness through a cAMP/NO- dependent mechanism.

Official Title
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Alcohol Exposure and Airway Hyperresponsiveness

Conditions
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Healthy

Intervention / Treatment
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* Other: ethanol


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: male must be of legal drinking age in the state of Nebraska (≥ 21) be between the ages of 21-65 be non-smokers be able to dedicate 3-4 hours on two consecutive days (including waiting at least 2 hours after the alcohol ingestion) able to provide informed consent Exclusion Criteria: female inability to give informed consent any history of lung or allergic disease any alcohol intake for the week prior to the experiment self-identified history of chronic heavy drinking or alcoholism or psychiatric disorder If an otherwise qualifying participant has previously undocumented or unidentified asthma as indicated by the baseline methacholine challenge, that subject will be excluded from the remainder of the study and replaced by another subject

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Post-alcohol change in airway hyperresponsiveness.<br>Participants will ingest 3 ounces of vodka mixed with fruit juice within 30 min. Then provocative concentration causing a 20% fall in forced expiratory volume in 1 s (PC20FEV1) will be measured. A one-half concentration difference in the PC20FEV1 will be considered a statistically significant change in airway hyperresponsiveness. | Other: ethanol<br>* subjects will ingest 3 ounces of vodka mixed with fruit juice within 30 min.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in airway hyperresponsiveness. | A one-half concentration difference in the PC20FEV1 will be considered a statistically significant change in airway hyperresponsiveness. | 2 hours |