Effect of Beta-blocker on Cardioprotective Effect of Remote Ischemic Conditioning

Study Overview
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Brief Summary
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Remote ischemic conditioning has been shown to protect myocardium from ischemia-reperfusion injury during cardiac intervention or cardiac surgery. However, effect of beta-blocker, commonly used cardiovascular medication in patients with cardiac diseases such as hypertension or angina pectoris, on cardioprotective role of remote ischemic conditioning has not been well documented. The purpose of the study is to investigate the effect of beta-blocker on remote ischemic conditioning in healthy volunteers.

Detailed Description
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This study is prospective cross-over study investigating effect of beta-blocker on cardioprotective role of remote ischemic conditioning. Eleven male healthy volunteers are going to take oral beta-blocker (carvedilol, 12.5 mg once) or not before undergoing remote ischemic conditioning (consisting of 4 cycles of 5-min ischemia and subsequent 5-min reperfusion of upper arm), separated by 6-day wash-out period. To evaluate cardioprotective effect of remote ischemic conditioning, blood samples will be obtained before and after remote ischemic conditioning. Form the samples, human dialysate will be obtained and be perfused to rat heart through Langendorff apparatus before ischemia-reperfusion injury to the rat heart. Changes of infarct size of the rat heart will be compared between the beta-blocker and control groups.

Official Title
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Effect of Beta-blocker on Cardioprotective Effect of Remote Ischemic Conditioning

Conditions
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Myocardial Infarction

Intervention / Treatment
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* Drug: Beta Blockers Carvedilol Phosphate


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy male volunteer Not taking any medication Exclusion Criteria: BMI < 18 kg/m2 or > 30 kg/m2 Allergic history of any medication Baseline SBP > 150 mmHg or < 100 mmHg Baseline DBP > 100 mmHg or < 50 mmHg Strenuous exercise, excessive caffeine or alcohol, smoking 24 h prior to experiment Cannot undergoing remote ischemic conditioning for any reason Refuse to enroll

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Beta Blockers Carvedilol Phosphate<br>Subjects are going to take beta-blocker (carvedilol, 12.5 mg once) before undergoing remote ischemic conditioning. Intervention: taking beta-blocker | Drug: Beta Blockers Carvedilol Phosphate<br>* Subjects are going to take Beta Blockers Carvedilol Phosphate (12.5mg once) before undergoing remote ischemic conditioning.<br>|
| No Intervention: Control<br>Subjects are going to undergo remote ischemic conditioning without taking any drug. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in infarct size of rat heart perfused with human dialysate after taking beta-blocker or not to evaluate the effect of beta blocker on remote ischemic conditioning performed to healthy volunteers | Infarct size measurement after perfusing the rat heart with human serum-derived dialysate and comparison before and after remote ischemic conditioning performed to healthy volunteers | 24 hour after remote ischemic conditioning to healthy volunteers | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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remote ischemic conditioning, beta-blocker