Anti-inflammatory Markers in Endometrioma

Study Overview
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Brief Summary
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Investigators aimed to measure the serum levels of adropin, salusin-α, netrin-1, and nesfatin-1, anti-inflammatory effects of which have been demonstrated previously, in endometriosis patients and to find out any association of them with insulin resistance.

Detailed Description
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Endometriosis is defined as the presence of endometrial gland and stroma outside the endometrial cavity affecting almost 10% of the women of reproductive age. Although benign, it disrupts the quality of life of the inflicted patients through symptoms such as dysmenorrhae, dyspareunia, chronic pelvic pain, and infertility. The pathogenesis of endometriosis has not yet been clearly elucidated, however, recent papers implies an underpinning role of inflammation There is a limited number of studies about the levels of these parameters in endometriosis and to date, no report is available on the levels of adropin and netrin-1 in endometriosis. Likewise, according to the investigator's literature search, no research explaining the association of endometriosis with insulin resistance has yet been published. In present study, in an attempt to describe inflammatory pathways playing a role in endometriosis pathogenesis, Investigators aimed to measure the levels of serum adropin, salusin-α, netrin-1, and nesfatin-1, anti-inflammatory effects of which have been priorly demonstrated, and to find out any association of them with insulin resistance.

Official Title
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The Role of Serum Adropin, Salusin-α, Netrin-1, and Nesfatin-1 in Endometrioma and Their Association With Insulin Resistance

Conditions
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Endometrioma, Inflammation

Intervention / Treatment
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* Diagnostic Test: Adropin (ng/L)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: ·Histopathologically confirmed diagnosis of endometrioma Exclusion Criteria: Any patients with DM, chronic kidney disease (CKD), CAD, heart failure (HF), cerebrovascular accident (CVA), malignancy, rheumatoid disorder, liver disease, and active infections were excluded from the study.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Endometrioma<br>Endometrioma (n=23) | Diagnostic Test: Adropin (ng/L)<br>* Thawed samples were measured on a Molecular Devices SpectraMax i3 Multi-Mode, Microplate Reader (batch number: SER 35 370-1448, made in Austria) using enzyme linked immunosorbent assay (ELISA).<br>* Other names: Nesfatin-1 (ng/mL);|
| Healthy controls<br>Healthy controls Healthy volunteers n=25 | Diagnostic Test: Adropin (ng/L)<br>* Thawed samples were measured on a Molecular Devices SpectraMax i3 Multi-Mode, Microplate Reader (batch number: SER 35 370-1448, made in Austria) using enzyme linked immunosorbent assay (ELISA).<br>* Other names: Nesfatin-1 (ng/mL);|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serum Adropin level | Serum Adropin level in ng/L | preoperative in the morning or Day 1 in outpatient clinic,preprandial for healthy volunteers | 
| Salusin-α | Serum Salusin-α level in pg/mL | preoperative in the morning or Day 1 in outpatient clinic, preprandial for healthy volunteers | 
| Netrin-1 | Serum Netrin-1 level in pg/mL | preoperative in the morning or Day 1 in outpatient clinic, preprandial for healthy volunteers | 
| Nesfatin-1 | Serum Nesfatin-1 level in ng/L | preoperative in the morning or Day 1 in outpatient clinic, preprandial for healthy volunteers | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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salusin-α, endometrioma, adropin, netrin-1, nesfatin-1, insulin resistance