High Dose Peripheral Blood Stem Cell Transplantation With Post Transplant Cyclophosphamide for Patients With Chronic Granulomatous Disease

Study Overview
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Brief Summary
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Chronic granulomatous disease (CGD) affects white blood cell function. Currently, the only curative treatment is bone marrow transplant to replace the abnormal stem cells with new ones (donor cells) capable of making a normal immune system. Transplant problems include graft versus host disease (GvHD) and graft rejection. With GvHD, donor cells attack the recipient s normal tissue. Researchers want to use preparation drugs and a high cell dose to increase graft success. They want to use 2 immunosuppressive drugs (cyclophosphamide and sirolimus) to lessen the risk of GvHD.

Detailed Description
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Study Description: Alemtuzumab, targeted busulfan, and TBI, with a 10/10 related or MUD donor graft or a 9/10 single HLA mismatch graft followed by post-transplant cyclophosphamide. Primary Objectives: To determine engraftment rates with the use of high cell doses, without increasing the risk of GvHD by using post-transplant cyclophosphamide and sirolimus in conjunction with a busulfan based conditioning regimen. We will compare the incidence of graft rejection/failure and GvHD to the incidence obtained from Protocol 07-I-0075. Secondary Objectives: To measure the engraftment rate and the engraftment kinetics using such a regimen. To assess the level and kinetics of immune reconstitution (via chimerism) when using post- transplant cyclophosphamide. To further elucidate the factors involved in the development of GvHD and graft rejection/failure. To evaluate the risk of viral infections in the setting of Alemtuzumab (Campath-1H) and post-transplant cyclophosphamide. Primary Endpoint: Reduced incidence of graft failure or rejection (as defined by >20% engraftment by oxidase- positive neutrophils in at least 95% of participants by Day 100, 6 months, and 1 year post BMT) will be assessed as event-free survival (EFS). Graft failure will result in disease recurrence. This will be assessed in a composite form along with GvHD (see Biostatistical Considerations section 17). Secondary Endpoints: Same or reduced rate of grade 3-4 aGvHD of <20% . Establish stable mixed chimerism. Improve rapidity of immune reconstitution. Overall survival. Tertiary Endpoints: Evaluation of inflammatory markers as risk factors for engraftment syndrome

Official Title
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High Dose Peripheral Blood Stem Cell Transplantation With Post Transplant Cyclophosphamide for Patients With Chronic Granulomatous Disease

Conditions
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Chronic Granulomatous Disease Transplant

Intervention / Treatment
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* Drug: Alemtuzumab
* Drug: Busulfan
* Drug: Sirolimus
* Drug: Cyclophosphamide
* Radiation: Total Body Irradiation
* Biological: Peripheral blood stem cells


Participation Criteria
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Eligibility Criteria
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INCLUSION CRITERIA: Must have confirmed Chronic Granulomatous Disease. Must have sufficient complications from underlying disease to warrant undergoing transplantation (either a history of or ongoing inflammation/CGD related autoimmunity OR a CGD related infection while on prophylaxis) OR or have a Quartile 1 and/or 2 residual oxidase production level. Ages 4 years - 65 years HLA-matched family donor graft or an HLA matched unrelated peripheral blood stem cell (PBSC) graft (10/10 or 9/10 mismatch) available Must be HIV negative When discharged from the hospital must be able to stay within one hour s travel of the NIH for the first 3 months after transplantation and have a family member or other designated companion to stay with during the post transplant period. Must provide a durable power of attorney for health care decisions to an appropriate adult relative or guardian in accordance to NIH -200 NIH Durable Power of Attorney for Health Care Decision Making . If of child-bearing potential, must agree to consistently use contraception from one month prior to, and throughout, study participation, and for 3 months post-study. Acceptable forms of contraception are: Contraceptive pills or patch, Norplant , Depo-Provera , or other FDA-approved contraceptive method Male partner has previously undergone a vasectomy. Male participants will be advised to consistently use contraception throughout study participation and for 3 months post-transplant. EXCLUSION CRITERIA: Eastern Cooperative Oncology Group (ECOG) or equivalent performance status greater than or equal to 3 (See Supportive Care guidelines, available at http://intranet.cc.nih.gov/bmt/clinicalcare) Left ventricular ejection fraction < 40% Transaminases > 5x upper limit of normal based on the participant s clinical situation and at the discretion of the investigator Psychiatric disorder or mental deficiency severe enough as to make compliance with the HSCT treatment unlikely, and/or making regulatorily and legally effective informed consent impossible Major anticipated illness or organ failure incompatible with survival from AlloPBSC transplant Pregnant or lactating HIV positive Uncontrolled seizure disorder Individuals older than 65 are excluded. It is known from standard transplantation that these participants have a higher risk of morbidity and mortality related to transplantation. Given the investigational nature of this protocol, the risk benefit ratio is not warranted to include these participants at this time. Any condition or circumstance which the PI feels would create difficulty in maintaining compliance with the requirements of this protocol. Individuals who are not willing to submit their information as part of the Alemtuzumab (Campath) Distribution Program application or participants whom the Distribution Program committee has determined are not qualified to receive alemtuzumab. --NOTE: Alemtuzumab (Campath-1H) (intravenous [IV] formulation) is no longer distributed commercially. In order to receive product, the physician must contact the program for the patient. If the patient is not willing to consent to submit their info (demographics, contact information, and rationale for use) to the program such that we can obtain the drug, then we cannot proceed with conditioning; therefore no transplant will occur on this protocol. Patients with a CRP greater than 100 mg/L within 30 days of anticipated transplant. If the underlying inflammation is controlled for one month with repeat CRP testing showing a level of less than 100 on at least two separate testings, the patient will be reconsidered for transplant. If during this time period a CRP of greater than 100 is measured, then the patient would no longer be eligible for transplant.

Ages Eligible for Study
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Minimum Age: 4 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: 1<br>There is only one treatment arm for this study | Drug: Alemtuzumab<br>* Transplant Conditioning Drug: Monoclonal antibody that targets recipient and donor T-cells to prevent graft verses host disease. Not an IND. This is a well studied drug, and is not under an IND.<br>Drug: Busulfan<br>* Transplant Conditioning Drug: Chemotherapy to create space in the patient's bone marrow so that the donor peripheral blood stem cells can repopulate in the patient's bone marrow. This is a well studied drug, and is not under an IND.<br>Drug: Sirolimus<br>* Immunosuppressant to prevent donor peripheral blood stem cell rejection and graft versus host disease. This is a well studied drug, and is not under an IND.<br>Drug: Cyclophosphamide<br>* Post transplant cyclophosphamide given to prevent graft verses host disease. This is a well studied drug, and is not under an IND.<br>Radiation: Total Body Irradiation<br>* Transplant Conditioning Total Body Radiation (300cGy in 2 fractionated doses), given only to patient receiving matched unrelated donor cells, to create space in the patient's bone marrow so that the donor peripheral blood stem cells can repopulate in the patient's bone marrow.<br>Biological: Peripheral blood stem cells<br>* Donor Peripheral blood stem cells, either matched unrelated donor or matched related relative to replace the patient's immune cells with functional immune cells. The peripheral blood stem cells are not regulated by the FDA.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To determine engraftment rates with the use of high cell doses, without increasing the risk of GvHD by using post-transplant cyclophosphamide and sirolimus in conjunction with a busulfan- based conditioning regimen. We will compare the incidence... | This study is still recruiting patients. | 5 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To measure the engraftment rate and the engraftment kinetics using such a regimen | This study is still recruiting patients | 5 years | 
| To assess the level and kinetics of immune reconstitution (via chimerism) when using post- transplant cyclophosphamide | This study is still recruiting patients | 5 years | 
| To further elucidate the factors involved in the development of GvHD and graft rejection/failure | This study is still recruiting patients | 5 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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The National Marrow Donor Program (NMDP), Matched Unrelated Donor (MUD), HLA Matched Related Donor