Macrophage Migration Inhibitory Factor (MIF)- Cardiac Surgery

Study Overview
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Brief Summary
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It has been repeatedly demonstrated that cardiac surgery with the use of extracorporeal circulation elicits a systemic inflammatory response and provokes ischemia-reperfusion related oxidative stress which could further lead to the development of organ failure in critically ill patients. Perioperative levels of macrophage migration inhibitory factor (MIF) and other inflammatory mediators could be involved in adverse outcomes in cardiac surgery.

Detailed Description
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The trace element selenium (Se) serves as an essential co-factor for the antioxidant enzymes Thioredoxin - reductase (TRR) and many other antioxidant enzymes, which protect tissues from oxidative stress. In septic patients it has previously been shown that thioredoxin, a TRR regulated Redox Enzyme, has the ability to suppress the synthesis of MIF. Aim of the present study is therefore to investigate the relation between perioperative selenium decrease and the reduction of TRR activity with a possible release of MIF during the inflammatory response after cardiac surgery.

Official Title
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Macrophage Migration Inhibitory Factor (MIF) as a Marker of Perioperative Inflammatory Reaction During Heart Surgery

Conditions
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Heart Surgery

Intervention / Treatment
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* Procedure: Blood sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: more than 18 years of age open heart surgery and use of heart-lung-device informed consent has been obtained Exclusion Criteria: under 18 years of age emergency cases informed consent cannot be obtained pregnancy or patient is breast-feeding

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Study Group<br>A single group of 100 consecutive patients will undergo additional blood sampling at different time points | Procedure: Blood sampling<br>* Blood sampling at various time points. Additional 92 mL blood.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Pilot study: correlation between clinical and lab parameters | To assess the importance of the MIF ligand/receptor family in a large cohort of patients (n=100) undergoing cardiac surgery and to characterize the underlying molecular events. Possible correlation between several clinical and laboratory parameters will be analyzed. This is a pilot study to generate hints at future items to be studied in controlled trials. | two months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Secondary Outcome | Correlation between perioperative MIF and MIF-2 levels and postoperative outcome Measurement of sCD74/MIF complex Determination of MIF polymorphism and its significance on clinical outcome perioperative inflammation organ injury | two months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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heart surgery