Macroscopic and Molecular Changes in Knee Muscular-tendon Unit in Response to Acute Resistant Load

Study Overview
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Brief Summary
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The aim of this study is to determine the influence of acute resistance exercises on anatomical and biological changes in the quadriceps muscle and tendons of the knee

Official Title
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Macroscopic and Molecular Changes in Knee Muscular-tendon Unit in Response to Acute Resistant Load

Conditions
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Quadriceps Muscle

Intervention / Treatment
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* Other: Acute resistant load exercises
* Behavioral: Background Questionnaire
* Other: Pain Questionnaire
* Genetic: DNA and RNA test
* Diagnostic Test: Ultrasound
* Diagnostic Test: Percutaneous Needle Core Biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Non-professional athletes Pass heart/lung endurance tests Pass ergometrics testing Exclusion Criteria: Have been injured Had physiotherapy for back or lower limbs in past 6 months Orthopedic surgery in lower limbs

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: One acute resistance load exercise<br>One hundred volunteers will participate in one acute resistance load exercises to their quadriceps | Other: Acute resistant load exercises<br>* Leg bench and leg press acute resistant load exercises Leg-bench and leg-press acute resistant load exercises<br>Behavioral: Background Questionnaire<br>* Background questionnaire will be filled out by participants<br>Other: Pain Questionnaire<br>* Pain questionnaire regarding pain in the knee during previous 3 months<br>Genetic: DNA and RNA test<br>* DNA and RNA tests will be sent to a genetic laboratory for analysis<br>Diagnostic Test: Ultrasound<br>* Ultrasound of the knee joint and the quadriceps muscle and the pica tendon<br>Diagnostic Test: Percutaneous Needle Core Biopsy<br>* Core Biopsy will be taken from the quadriceps femoris muscle<br>|
| Active Comparator: Three acute resistance load exercises<br>One hundred volunteers will participate in three acute resistance load exercises to their quadriceps | Other: Acute resistant load exercises<br>* Leg bench and leg press acute resistant load exercises Leg-bench and leg-press acute resistant load exercises<br>Behavioral: Background Questionnaire<br>* Background questionnaire will be filled out by participants<br>Other: Pain Questionnaire<br>* Pain questionnaire regarding pain in the knee during previous 3 months<br>Genetic: DNA and RNA test<br>* DNA and RNA tests will be sent to a genetic laboratory for analysis<br>Diagnostic Test: Ultrasound<br>* Ultrasound of the knee joint and the quadriceps muscle and the pica tendon<br>Diagnostic Test: Percutaneous Needle Core Biopsy<br>* Core Biopsy will be taken from the quadriceps femoris muscle<br>|
| Active Comparator: Control Group<br>One hundred volunteers will participate in study as a control group | Behavioral: Background Questionnaire<br>* Background questionnaire will be filled out by participants<br>Other: Pain Questionnaire<br>* Pain questionnaire regarding pain in the knee during previous 3 months<br>Genetic: DNA and RNA test<br>* DNA and RNA tests will be sent to a genetic laboratory for analysis<br>Diagnostic Test: Ultrasound<br>* Ultrasound of the knee joint and the quadriceps muscle and the pica tendon<br>Diagnostic Test: Percutaneous Needle Core Biopsy<br>* Core Biopsy will be taken from the quadriceps femoris muscle<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Genetic Changes in Knee Muscle and Tendon | Comparison of results of genetic testing both before and after interventions between the 3 groups | One year | 
| Ultrasound Changes in Knee Muscle and Tendon | Comparison of results of pre- and post ultrasound changes after interventions between the 3 groups | One year | 
| Biopsy Changes in Knee Muscle and Tendon | Comparison of changes in biopsies taken before and after interventions between the 3 groups | One year |