Mitochondrial Capacity in Pregnant Women

Study Overview
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Brief Summary
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This Pilot & Feasibility study will test the hypothesis that pregnant women who are more active will have better functioning mitochondria in vivo (higher ATPmax, via 31P-MRS), which will be positively related to maternal and infant mitochondrial function in vitro (higher oxygen consumption rates in primary myotubes and umbilical cord derived mesenchymal stem cells, respectively). Twenty pregnant women (10 active and 10 sedentary) will be recruited in the first trimester of pregnancy determined from self-reported physical activity and assessment by activity monitors. Placental biospecimens will be collected during delivery for other molecular mitochondrial analyses.

Official Title
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The Effect of Physical Activity on in Vivo and in Vitro Mitochondrial Capacity in Pregnant Women

Conditions
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Pregnancy Related, Physical Activity

Intervention / Treatment
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* Behavioral: Active
* Behavioral: Sedentary


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Pregnant female BMI between 18.5 kg/m2 and 30.0 kg/m2 prior to the current pregnancy (determined by self report and confirmation of pregravid BMI of the index pregnancy from the prenatal record) Medically cleared for participation in the study by primary care obstetrician or midwife Medically cleared for participation by the Medical Investigator Medical record release (prenatal record, hospital delivery record) for study staff to access information in the medical record related to the current and if applicable, the prior pregnancy. Exclusion Criteria: Risk of early delivery (eg. history of preterm birth, spontaneous abortion, evidence of gestational hypertension (SBP >160 mmHg & DBP >110 mmHg) or pre-eclampsia in the current pregnancy) History of intrauterine growth-restriction Persistent second- or third-trimester bleeding Placenta previa after 26 weeks gestation Incompetent cervix or cerclage Severe anemia High-order pregnancy (e.g. triplets) Family history of diabetes Heart or lung disease HIV or AIDS (self-reported) Individuals with contraindication to magnetic resonance spectroscopy such as having magnetic metallic objects in their body Unwillingness for placental biospecimens to be collected at delivery

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active<br>Physically active will be defined by self-report and confirmed by step counts >8,000 per day from activity monitoring. | Behavioral: Active<br>* No behavioral intervention included but the exposure is level of physical activity. Individuals in the Active group will be categorized by their own routine physical activity levels.<br>|
| Sedentary<br>Sedentary lifestyle will be defined by self-report and confirmed by step counts <5,000 per day from activity monitoring. | Behavioral: Sedentary<br>* No behavioral intervention included but the exposure is level of physical activity. Individuals in the Sedentary group will be categorized by their own routine physical activity levels.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Infant in vitro mitochondrial capacity (by oxygen consumption rate) | Infant in vitro mitochondrial capacity measured by oxygen consumption rates in umbilical cord derived mesenchymal stem cells at delivery | 1 day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Relationship between in vivo mitochondrial capacity and infant in vitro mitochondrial capacity (by oxygen consumption rates) | Relationship between in vivo and in vitro mitochondrial capacity will be measured with in vivo mitochondrial capacity measured by 31P-MRS and infant in vitro mitochondrial capacity measured by oxygen consumption rates in umbilical cord derived mesenchymal stem cells | About 20 weeks | 
| Relationship between in vivo mitochondrial capacity and maternal in vitro mitochondrial capacity (by oxygen consumption rate) | Relationship between in vivo and in vitro mitochondrial capacity will be measured with in vivo mitochondrial capacity measured by 31P-MRS and maternal in vitro mitochondrial capacity measured by oxygen consumption rates in maternal primary myotubes | About 15 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Pregnancy, Mitochondrial metabolism, Placenta