Diet Oil Induced Stimulation of GLP-1

Study Overview
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Brief Summary
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Activation of G protein-coupled receptor GPR119 stimulates glucagon-like peptide-1 (GLP-1)release from the intestinal L-cells. Previously, administration of 2-oleyl-glycerol (2-OG) to humans significantly increased plasma GLP-1. In the present study we want to test the effect in patients with type 2 diabetes. The hypothesis is that we will expect to find a significant increased plasma GLP-1 following a meal containing of 2-OG when compared to meals containing of olive oil or carbohydrates alone.

Detailed Description
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Activation of G protein-coupled receptor GPR119 stimulates glucagon-like peptide-1 (GLP-1)release from the intestinal L-cells. Previously, administration of 2-oleyl-glycerol (2-OG) to humans significantly increased plasma GLP-1. In the present study we want to test the effect in patients with type 2 diabetes. The hypothesis is that we will expect to find a significant increased plasma GLP-1 following a meal containing of 2-OG when compared to meals containing of olive oil or carbohydrates alone. The aim of the study is to elucidate the effect of 2-OG, 'diet oil' on GLP-1 secretion in patients with type 2 diabetes and to explain the mechanism behind the known stimulation of GLP-1 release by dietary fat.

Official Title
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Diet Oil Induced Stimulation of GLP-1

Conditions
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Type 2 Diabetes Mellitus

Intervention / Treatment
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* Dietary Supplement: Diet oil
* Dietary Supplement: Olive oil
* Dietary Supplement: Carrot


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients with type 2 diabetes mellitus according to the WHO criteria caucasians age above 18 years BMI 20-30 Exclusion Criteria: kidney disease liver disease retinopathy anaemia use of insulin use of GLP-1 analogues use of DPP-4 inhibitors

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Diet oil<br> | Dietary Supplement: Diet oil<br>* Carrot and diet oil (containing 2-OG) and 1½ g of paracetamol<br>|
| Active Comparator: Olive oil<br> | Dietary Supplement: Olive oil<br>* Carrot and olive oil and 1½ g of paracetamol<br>|
| Placebo Comparator: Carrot<br> | Dietary Supplement: Carrot<br>* Carrot and 1½ g of paracetamol<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma GLP-1 | Glp-1 response is measured during and three hours after ingestion of the different mealtest | 3 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma insulin | Plasma insulin is measured during and 3 hours after mealtest | 3 hours | 
| Plasma glucose | Plasma glucose is measured during and 3 hours after meal test | 3 hours | 
| Plasma GIP | Plasma GIP is measured during and 3 hours after meal test | 3 hours | 
| Plasma glucagon | Plasma glucagon is measured during and 3 hours after meal test | 3 hours | 
| Plasma PYY | Plasma PYY is measured during and 3 hours after meal test | 3 hours | 
| Plasma paracetamol | Plasma paracetamol is measured during and 3 hours after meal test | 3 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Glucagon-like peptide-1, Glucose-dependant insulinotropic polypeptide, Glucagon, Peptide PYY, 2-oleyl glycerol