People Living With HIV/AIDS, Nitrate, Endotelial Function, Arterial Stiffness and Skeletal Muscle Oxygenation

Study Overview
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Brief Summary
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Administration of antiretroviral therapy has brought benefits for infective human immunodeficiency virus-1 (HIV-1)-patients, reducing the number of AIDS-related morbidity and mortality. However, it is responsible for intensifying complications triggered by the chronic inflammatory state associated with virus infection, such as cardiovascular diseases. The current study evaluated effects of single dose (140mL) of Beetroot Juice (BJ) on macro and microvascular endothelial function in HIV-1-patients undergoing antiretroviral terapy, once BJ was shown to be effective in improving these physiological parameters in uninfected individuals.

Official Title
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Effect of Ingestion of Beet Juice on Endothelial Function, Arterial Stiffness and Muscle Oxygenation Parameters in People Living With HIV / AIDS Using Antiretroviral Therapy

Conditions
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HIV/AIDS

Intervention / Treatment
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* Dietary Supplement: Beetroot Juice
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: HIV infection for a period of 1 year or greater Regular use of high-potency antiretroviral therapy for at least one year Exclusion Criteria: Individuals who are physically active Individuals who are allergic to some component of beet Individuals who use nutritional and / or pharmacological ergogenics Individuals receiving antibiotics or who have osteomioarticular problems

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 59 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Beetroot juice<br>Beetroot Juice cotaining on average 9mmol of nitrate per dose | Dietary Supplement: Beetroot Juice<br>* 140ml of Beetroot Juice containing on avarege 9 mmol of nitrate<br>|
| Placebo Comparator: Placebo beet juice (Nitrate depleted)<br>Beetroot juice nitrate depleted | Dietary Supplement: Placebo<br>* 140ml of Beetroot Juice (nitrate free)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Skeletal Muscle Oxygenation | Changes in skeletal muscle oxygenation in response to exercise will be evaluated by using a near-infrared spectroscopy device. | Baseline and approximately after 150 minutes of beetroot juice consumption | 
| Brachial artery flow-mediated dilatation | The brachial artery flow-mediated dilation will be evaluated by using an ultrasound device. | Baseline and approximately after 150 minutes of beetroot juice consumption | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Urinary nitrate concentrations | Analysis of nitrate in urine will be performed by using high-performance liquid chromatography system. | Baseline and approximately 30, 90, and 150 minutes after beetroot juice consumption | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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HIV/AIDS, Arterial stiffness, Flow mediated-dilatation, Muscle oxygenation parameters, Microcirculation, Dietary Nitrate