Smoker Extracellular Vesicles Influence on Human Bronchial Epithelial Cells

Study Overview
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Brief Summary
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Cigarette smoking is a habit that has spread all over the world and is a significant risk factor for many diseases including cardiovascular disease, chronic obstructive pulmonary disease(COPD),asthma and lung cancer. Evaluation and understanding of tobacco health effects are of major interest worldwide and answer to important societal concerns. Identification of new biomarkers of exposure to tobacco smoke potentially implicated in COPD or lung carcinogenesis would allow a better observation of tobacco exposed population, thanks to screening establishment at reversible stages of pathological processes. In this study, we question whether cigarettes smoking alters miRNA profiles of extracellular vesicles (EVs) present in human broncho alveolar lavages (BALs), which could affect surrounding normal bronchial epithelial cells status.

Detailed Description
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Extracellular vesicles (EVs) include a variety of nanoscale membranous vesicles (exosomes, microvesicles, microparticles). EVs are released into the interstitial fluid from a wide variety of normal or diseased cells. Analysis of EVs and their content maybe useful as disease biomarkers as they reflect the contents of cells of origin, differ between normal and diseased tissue and can be reliably detected. EVs may thus act as biomarkers of diverse pathologies like cancer, and detection of these biomarkers maybe applied to early diagnosis or assessment of prognosis in patients with cancer. EVs indeed contain both mRNAs and non-coding RNAs, such as small regulatory microRNAs (miRNAs).

Official Title
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Particulate Air Pollution : Smoker Extracellular Vesicles Influence on Human Bronchial Epithelial Cells

Conditions
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Human Bronchial Epithelial Cells, Lung Pathogenesis, Biomarkers, Smokers

Intervention / Treatment
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* Diagnostic Test: Broncho Alveolar Lavages (BAL)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: >18 year-old Exclusion Criteria: Lack of informed consent Drug treatment Professional exposure Evolutive pregnancy Bradycardia Respiratory assistance required, Diagnosed respiratory distress (e.g. COPD, asthma) Infectious pneumopathy Bronchial cancer

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Smokers<br>Smokers outpatients at the Pneumology service from the HôpitalSaint-Philibert (Lomme, France) to whom a bronchoalveolar lavage will be performed | Diagnostic Test: Broncho Alveolar Lavages (BAL)<br>* BEAS-2 B cells are exposed to EVs isolated from BAL of smokers and non-smokers.<br>|
| Active Comparator: Non smokers<br>Non smokers outpatients at the Pneumology service from the HôpitalSaint-Philibert (Lomme, France) to whom a bronchoalveolar lavage will be performed | Diagnostic Test: Broncho Alveolar Lavages (BAL)<br>* BEAS-2 B cells are exposed to EVs isolated from BAL of smokers and non-smokers.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Quantification of target mRNA expression in human bronchial epithelial cells (BEAS-2 B) exposed to EVs isolated from LBA of patients | Quantitative polymerase chain reaction (RT-qPCR) for determination of mRNA expression | Through the study completion, an average of 48 months |