Circulating Non-coding RNA in Acute Ischemic Stroke (AISRNA)

Study Overview
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Brief Summary
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AISRNA is to analyze the expression pattern of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA) by next-generation sequencing in patients with acute ischemic stroke and healthy control. The candidate circRNA/miRNA/lncRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke.

Detailed Description
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Noncoding RNAs have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of this protocol will investigate the expression pattern of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA) by next-generation sequencing in patients with acute ischemic stroke and healthy control. The candidate circRNA/miRNA/lncRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke. Distinctive expression patterns of circRNA/miRNA/lncRNA will be identified by the next-generation sequencing and individual quantitative real time polymerase chain reaction (qRT-PCR). A diagnostic or predictive model will be established using logistic regression. The panel of these altered ncRNAs may be associated with acute IS and could serve as a novel diagnostic or predictive method.

Official Title
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Clinical Significance of Circulating Non-coding RNA in Acute Ischemic Stroke (AISRNA)

Conditions
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Ischemic Stroke, Acute Stroke

Intervention / Treatment
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* Genetic: Sequencing of circRNA/miRNA/lncRNA


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Aged 18 years or older Confirmed acute ischemic stroke by a diffusion-weighted imaging-position lesion on magnetic resonance imaging (MRI) and a new lesion on a brain computed tomography (CT) scan Within 72 hours of symptom onset Good performance status Signed an approved informed consents Exclusion Criteria: -a history of hemorrhagic infarction, chronic kidney/liver diseases, peripheral arterial occlusive disease, active malignant disease, and inflammatory or infectious diseases

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| AIS group<br>This group includes patients with acute ischemic stroke (AIS). | Genetic: Sequencing of circRNA/miRNA/lncRNA<br>* Next generation sequencing of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA)<br>* Other names: Quantitative Real-time polymerase chain reaction;|
| HC group<br>This group includes healthy controls (HC). | Genetic: Sequencing of circRNA/miRNA/lncRNA<br>* Next generation sequencing of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA)<br>* Other names: Quantitative Real-time polymerase chain reaction;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Differential expression pattern of circRNA/miRNA/lncRNA | Differential expression pattern of circular RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA) will be compared between AIS group and HC group, thus candidate circRNA/miRNA/lncRNA will be verified as biomarkers for the detection and prognosis of AIS. | 90 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Prognostic value of circRNA/miRNA/lncRNA in acute ischemic stroke | The prognosis of AIS patients will be analyzed between differential expressed candidate miRNA/lncRNA after they have completed the systematic therapy. | 90 days | 
| Correlation of circRNA/miRNA/lncRNA and inflammatory factors in acute ischemic stroke | Correlation of circRNA/miRNA/lncRNA and inflammatory factors on admission will be explored in acute ischemic stroke. | 7 days | 
| Correlation of circRNA/miRNA/lncRNA and stroke-associated infection | Correlation of circRNA/miRNA/lncRNA and stroke-associated infection will be explored in acute ischemic stroke. | 30 days | 
| Dynamic changes of circRNA/miRNA/lncRNA during the follow-up period | Dynamic changes of circRNA/miRNA/lncRNA will be explored before and after endovascular therapy | 90 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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acute ischemic stroke, noncoding RNA, clinical significance, outcome