Effect of Cock's Comb Extract in the Treatment of Arsenical Skin Lesion

Study Overview
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Brief Summary
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Cock's comb extract is rich in hyaluronic acid, which is used as viscosupplementation injection and oral supplementation for osteoarthritis. Hyaluronic acid acts as humectants and topical moisturizing agent to the skin due to excellent moisturizing property. It has anti-inflammatory effect and used in the treatment of ulcer healing and to reduce the intensity of radioepithelitis. It is also used as a topical vehicle for the delivery of drug to the skin in actinic keratosis. In the case of arsenical keratotic nodule, topical application of hyaluronic acid may tends to decrease keratosis by repeated hydration of the skin. Therefore, the study will be conducted to determine any beneficial effect of topical application of cock's comb extract in the treatment of the patients with severe palmar arsenical keratosis.

Detailed Description
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Cock's comb extract is rich in hyaluronic acid, which is used as viscosupplementation injection and oral supplementation for osteoarthritis. Hyaluronic acid is extensively utilized in many cosmetic products because of its moisturizing property as a topical moisturizing agent. It is also used as dermal fillers for wrinkle correction. It has anti-inflammatory effect and used in the treatment of different type of ulcers. In case of acute radioepithelitis, 0.2% hyaluronic acid is used to reduce inflammation. Hyaluronic acid has found important application in drug delivery system. Recently 3% diclofenac in 2.5% hyaluronic acid gel has been approved for the treatment of actinic keratoses as a unique topical vehicle for the localized delivery of drug to the skin. It is also used as a vehicle for various anticancer drugs. As it has many effective topical applications, it can be used in arsenical keratosis. In the case of arsenical keratotic nodule, topical application of hyaluronic acid may cause repeated hydration of the skin as a result keratosis tends to soften and disappear. Therefore, the study will be conducted to identify the outcome of cock's comb extract in the treatment of arsenical skin lesion. Biological activity and cytotoxicity of cock's comb extract have been identified after performing brine shrimp bioassay test. Twenty-five patients with severe palmar (>5 mm) arsenical keratosis will be recruited on the basis of inclusion and exclusion criteria. Before the study, water and nail samples will be collected to confirm arsenicosis. Blood samples will be collected for liver and kidney function test to observe any adverse effects of topically applied cock's comb extract both before and after the study. The cream will be prepared with 0.01% cock's comb extract mixed with olive oil, bee wax and benzoic acid for topical application 2 days prior visit of the recruited patients. The patient will be applied the cream on the affected area with a clean fingertip by rubbing gently at twice daily for 12 weeks without any interruption. Primary parameters will be the measurement of keratotic nodular size by slide calipers and perception scoring of the patients by Likert scale before and 12 weeks after the study. Secondary parameters will be the measurement of arsenic level in drinking water and nails of the recruited patients.

Official Title
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Effect of Cock's Comb Extract in the Treatment of Arsenical Skin Lesion

Conditions
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Chronic Arsenic Poisoning

Intervention / Treatment
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* Drug: Cock's comb extract 0.01%


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: (Patients) Age: 18-60 years Sex: Both male and female Arsenical keratosis: Presence of severe keratosis (>5mm) in both palms Drinking arsenic contaminated water (>50 µg/L) for at least more than 6 months Patient voluntarily agreed to participate Patient who understood the instruction of applying drug and could apply drug as per as instruction Exclusion Criteria: Age <18 and >60 years Pregnant and nursing mother Palmar psoriasis Eczema Any kind of systemic disease, inflammatory disease and infectious condition that affect skin for example diabetes mellitus, rheumatoid arthritis, systemic lupus erythrematosus, hepatitis Hypersensitivity to avain protein Malignancy Impaired renal function - if creatinine level >1.3 mg/dl incase of men and >1.1 mg/dl in case of women Patient who received any treatment of arsenicosis within last three months

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Interventional Model Description: Topical application of cock's comb extract in the treatment of palmar arsenical keratosis
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Cock's comb extract 0.01%<br>25 Patients Cock's comb extract 0.01% Applied topically twice daily for 12 weeks | Drug: Cock's comb extract 0.01%<br>* Applied topically twice daily for 12 weeks<br>* Other names: Cock's comb extract cream;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in palmer arsenical keratosis | Size of keratotic lesion will be decreased | [0 week (baseline), 12 weeks (end)] [ Safety Issue: No]] [Safety Issue: No] | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in arsenic level in nail | Arsenic level in nail will be decreased | [0 week (baseline), 12 weeks (end)] [ Safety Issue: No]] [Safety Issue: Yes] |