VEGF Signaling Promotes Cell Growth and Metastasis in Intrahepatic Cholangiocarcinoma in a VEGF Receptor Mediated Pathway

Study Overview
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Brief Summary
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The investigators study the VEGF signaling in ICC cell lines／human tissues and its mechanism in HCC growth, proliferation and apoptosis.

Detailed Description
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Several studies have documented the elevated expression of VEGF and its receptors in ICC cell lines and tumor tissues. Another study observed that the elevation of VEGF promotes the growth. Clinical analyses have discovered the high expression of VEGF is correlated with tumor progression, vascular invasion, distal metastasis and poor prognosis. However, few studies have been conducted to investigate the VEGF signaling in ICC cells and its possible mechanism in regulating ICC growth.Therefore,we try to clarify the mechanism of VEGF signal in ICC growth,proliferation and apoptosis.

Official Title
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VEGF Signaling Promotes Cell Growth and Metastasis in Intrahepatic Cholangiocarcinoma in a VEGF

Conditions
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Intrahepatic Cholangiocarcinoma

Intervention / Treatment
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* Procedure: surgical treatment


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: hepatocellular patients diagnosed through biopsy;or either dynamic imagine with a diagnosis of intrahepatic cholangiocarcinoma and CA199>100U/L；or two or more dynamic imagine with a diagnosis of intrahepatic cholangiocarcinoma Child-Pugh A or B well preserved renal and hematopoietic Function receive complete surgical therapy achieve complete section accessed by contrast-enhanced CT Exclusion Criteria: incomplete section Child-Pugh C combination with other hepatobiliary disease suffer from other tumors concurrently or in last five years

Ages Eligible for Study
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Minimum Age: 16 Years
Maximum Age: 90 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| ICC patients<br>patients with intrahepatic cholangiocarcinoma treated by surgical treatment | Procedure: surgical treatment<br>* all the patients in the study must have been treated by the surgery at the first time and have been confirmed as 'complete section' by the post-surgery radiological image.<br>* Other names: hepatectomy;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| the variation of the quantity of VEGF signal pathway genes in normal liver tissue,peri tumor,tumor and metastasis. | the sections would be collected as previously described.Western blot,immunohistochemistry,PCR would be conducted to find out the variation between the normal liver tissue and tumor,tumor and meta(if have). | the day when conducting surgery (day 1) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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intrahepatic cholangiocarcinoma, icc, vegf signal pathway