Relationship Between NLR and Prealbumin Levels With Diaphragm Thickness

Study Overview
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Brief Summary
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A study to find the relationship between neutrophil to lymphocyte ratio (NLR) with diaphragm thickness of critical patients in ICU.

Detailed Description
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Weaning from ventilator sometimes can be failed and it happened due to several factors. One of the causes was Ventilator Induced Diaphragm Dysfunction (VIDD). This VIDD process occurred due to thinning process of the diaphragm thickness or decreased diaphragm muscle mass. The whole process happened due to inflammatory reaction that usually appeared on patients in Intensive Care Unit (ICU). Thus, this study was aimed to find the relationship between neutrophil to lymphocyte ratio (NLR) with diaphragm thickness of critical patients in ICU.

Official Title
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Relationship Between Neutrophil to Lymphocyte Ratio and Prealbumin Serum Levels With Diaphragm Thickness in Critical Patients

Conditions
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Inflammatory Response

Intervention / Treatment
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* Diagnostic Test: Ratio of Neutrophil to Lymphocyte
* Diagnostic Test: Level of Prealbumin Serum


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Critical patients treated in intensive care unit using mechanical ventilation from day 0. Patients are clinically predicted to be treated for more than 5 days (qSOFA and clinical assessment) Exclusion Criteria: History of suffering from neuromuscular disease and or diaphragmatic malformations Used non-invasive ventilation before starting to use mechanical ventilation History of tracheostomy Patients admitted to ICU within 12 months before this study

Ages Eligible for Study
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Minimum Age: 17 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Normal Diaphragm<br>Diaphragm thickness is 2 mm or more | Diagnostic Test: Ratio of Neutrophil to Lymphocyte<br>* Each patient was examined for a blood sample of 6 ml of venous blood taken from the cubital vein using a vacuum tube without anticoagulants. Blood samples were centrifuged at a speed of 3500 rpm for 20 minutes. Samples are processed with abbott® devices, to obtain neutrophil and serum lymphocyte levels. Plasma samples are stored in the refrigerator -20 C. The patient's diaphragm thickness was measured on days 0, 3, 5 and then the measurement was compared. Diaphragm thickness was measured in patients using mechanical ventilation in intensive care rooms.<br>Diagnostic Test: Level of Prealbumin Serum<br>* Each patient was examined for a blood sample of 6 ml of venous blood taken from the cubital vein using a vacuum tube without anticoagulants. Blood samples were centrifuged at a speed of 3500 rpm for 20 minutes. Samples are processed with abbott® devices, to obtain neutrophil and serum lymphocyte levels. Plasma samples are stored in the refrigerator -20 C. The patient's diaphragm thickness was measured on days 0, 3, 5 and then the measurement was compared. Diaphragm thickness was measured in patients using mechanical ventilation in intensive care rooms.<br>|
| Thinning Diaphragm<br>Diaphragm thickness is less than 2 mm | Diagnostic Test: Ratio of Neutrophil to Lymphocyte<br>* Each patient was examined for a blood sample of 6 ml of venous blood taken from the cubital vein using a vacuum tube without anticoagulants. Blood samples were centrifuged at a speed of 3500 rpm for 20 minutes. Samples are processed with abbott® devices, to obtain neutrophil and serum lymphocyte levels. Plasma samples are stored in the refrigerator -20 C. The patient's diaphragm thickness was measured on days 0, 3, 5 and then the measurement was compared. Diaphragm thickness was measured in patients using mechanical ventilation in intensive care rooms.<br>Diagnostic Test: Level of Prealbumin Serum<br>* Each patient was examined for a blood sample of 6 ml of venous blood taken from the cubital vein using a vacuum tube without anticoagulants. Blood samples were centrifuged at a speed of 3500 rpm for 20 minutes. Samples are processed with abbott® devices, to obtain neutrophil and serum lymphocyte levels. Plasma samples are stored in the refrigerator -20 C. The patient's diaphragm thickness was measured on days 0, 3, 5 and then the measurement was compared. Diaphragm thickness was measured in patients using mechanical ventilation in intensive care rooms.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Level of Prealbumin Serum |  | 5 days | 
| Ratio of Neutrophil to Lymphocyte |  | 5 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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diaphragm thickness, neutrophil to lymphocyte ratio, mechanical ventilation