Microbial Epidemiology and Chlorhexidine Suscebtibily of Oropharyngeal and Intestinal Colonization

Study Overview
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Brief Summary
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In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in healthy subjects, and their susceptibility to chlorehexidine. We plan to recruit 100 healthy volunteers secondary endpoints are to determine the phylogentic characteristics of E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal of predominant GNB colonization.

Detailed Description
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In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in 100 healthy subjects, and their susceptibility to chlorehexidine. Each participant will undego a oropharyngeal swab collection and a rectal swab at day 0. The Gram-negative bacilli colonization will be identified, E. coli isolates will be studied in terms of phylogeny; gram-negative isolates will be studied in terms of antimicrobial and chlorhexidine susceptibility. The primary objective is to determine the prevalence of oropharyngeal Gram-negative bacilli colonization. The secondary objectives are to determine the chlorhexidine and antimicrobial susceptibility of such colonization; to determine the phylogentic characteristics of oropharyngeal and rectal predominant E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal E. coli isolates.

Official Title
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Comparative Microbial Epidemiology and Chlorhexidine Suscebtibily of Oropharyngeal and Intestinal Colonization of Healthy Adults Subjects

Conditions
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Oropharyngeal Gram-negative Bacilli Colonization

Intervention / Treatment
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* Other: oropharyngeal swabbing
* Other: rectal swabbing


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age > 18 yo personal history of neck or face irradiation no history of gastrointestinal disease no symptoms of immunosuppression no antibiotic therapy in the previous month no hospitalization in the 3 months preceding inclusion medical exmination prior to inclusion Exclusion Criteria: affiliated to the social security The refusal of a patient to participate in a study

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| healthy adult subject<br>oropharyngeal and rectal swabbing to collect Gram-negative bacilli | Other: oropharyngeal swabbing<br>* microbiological sampling will be performed on buccal mucosa with standard swab. only one sample will be done<br>Other: rectal swabbing<br>* same process as oropharyngeal swabbing will be used to collect rectal microbiological sample<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| proportion of patients with an oropharyngeal gram-negative colonization |  | Day 1 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| incidence of the a GNB oropharyngeal colonization |  | Day 1 | 
| comparision of the predominant fecal flora with GNB oropharyngeal colonization |  | Day 1 | 
| characterization of antimicrobial and chlorhexidine susceptibility of Gram negative bacteria from oropharyngeal colonization |  | Day 1 | 
| phylogenetic and virulence factor content of E. coli oropharyngeal and rectal colonization |  | Day 1 |