Study to Evaluate the Effect of Botanical Dietary Supplements on Inflammation in Healthy People

Study Overview
=================
Brief Summary
-----------------
The purpose of the study is to evaluate the effect of botanical dietary supplements on inflammation in healthy people with different genetic responses to inflammation.

Detailed Description
-----------------
The impact of nutrition and lifestyle have long been recognized as key determinants of risk for chronic degenerative diseases such as atherosclerosis, osteoporosis, rheumatoid arthritis, etc. While it is clear that reducing nutrition and lifestyle risk factors for a particular disease reduces risk in populations, individuals differ significantly in the degree to which presumed beneficial changes in these risk factors confer risk or lack thereof. This individual response is increasingly thought to be due genetic differences. Developing in parallel with the research on genetic impact and chronic disease risk is a strengthening body of data suggesting that inflammation is common to many chronic degenerative diseases. A key cytokine regulator of the inflammatory response, interleukin-1 (IL-1), has emerged as playing a particularly important role at the genetic level in determining the degree to which the inflammation pathway is turned on. The strong influence of IL-1 over the inflammation pathway follows from its functional role as one of the initiating cytokine signals in the inflammatory pathway. Recent research has identified polymorphisms in the IL-1 gene that lead to over expression of IL-1 and elevated levels of the inflammation biomarker, c-reactive protein (CRP). Individuals with selected polymorphisms associated with over expression of IL-1 appear to be at increased risk for selected chronic degenerative diseases. The mechanistic role of IL-1 in the overall inflammatory response and the detrimental impact of IL-1 over-expression may create a need for IL-1 genotype-directed nutritional, lifestyle and dietary supplement interventions that address the individual genetic risk for inflammation associated diseases by reducing chronic inflammation. The current protocol draws participants from a pre-existing database containing information on health and lifestyle, IL-1 genotype, and inflammation biomarkers from approximately 2300 people. The database was created under a separate IRB-approved protocol. Participants will randomly receive one of three botanical formulas or a placebo once a day for 20 weeks. The effects of the botanical formulas on recognized markers of inflammation, e.g., CRP, as well as surrogate readouts of the inflammation pathway, ex vivo IL-1 production, and in vivo expression of the IL-1 gene will be evaluated.

Official Title
-----------------
Double-Blind Trial to Evaluate the Effect of Botanical Dietary Supplements on C-Reactive Protein, Ex-Vivo IL-1 Production, and In-Vivo IL-1 Gene Expression in Healthy Human Subjects

Conditions
-----------------
Healthy

Intervention / Treatment
-----------------
* Drug: Rose hips extract + blueberry/blackberry extract + resveravine
* Drug: Rose hips extract + blueberry/blackberry extract + Aframomum melegueta extract
* Drug: Rose hips extract + resveravine + Aframomum melegueta extract


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Non-smoking adults aged 18 and up Individual should be judged to be in good general health on the basis of an interview, vitals assessment, and physical examination Individual is willing to maintain their normal dietary and exercise habits throughout the duration of the trial Individual understands the procedures and agrees to participate in the study Individual is able and willing to provide written informed consent Average serum CRP level between 2 and 10 mg/L inclusively. Exclusion Criteria: Current smoker or history of tobacco use within the past year Use of dietary supplements within one week of Day 1. Supplements include any vitamins, minerals, and herbal products, including herbal drinks Presence of, or clinical significant history of, autoimmune, blood, cancer, cardiovascular, endocrine, kidney, liver, lung, gastrointestinal, metabolic disorder, and/or any other chronic health condition identified from the findings of the interview Currently treated for uncontrolled hypertension or blood pressure > 170 mm Hg systolic or > 100 mm Hg diastolic during seated, resting measurement on two consecutive occasions during visit 1 Therapeutic use of nitroglycerin, alpha1- or beta-adrenergic blockers, Apresoline, Loniten, calcium channel blockers, ACE inhibitors, and/or any other drug that may alter blood pressure Therapeutic use of coumadin, aspirin, or other medications that influence blood coagulation Current use of NSAIDS, including COX-2 inhibitors Therapeutic use of cholesterol-lowering medications such as HMG CoA reductase inhibitors, bile acid binding agents, bile acid binding resins, nicotinic acid, and fibric acid derivatives Known allergy to rose hips, blueberries, blackberries, resveravine, and/or A. melegueta Current use of any form of steroid drug (prescription or non-prescription), including inhalers for asthma Current use of any form of hormone replacement therapy (HRT and ERT) Participation in another clinical trial within 30 days of enrollment into the study History or current abuse of drugs or alcohol, or intake > 2 alcoholic beverages per day Pregnant or lactating women, or women of child-bearing potential unwilling to use a medically approved form of birth control Any condition that the Principal Investigator believes may put the subject at undue risk Serum CRP less than 3 or higher than 10 mg/L. If a subject has a CRP > 10 mg/L at screening, the measurement will be repeated two weeks later to determine whether the subject should be excluded from the protocol

Ages Eligible for Study
-----------------
Minimum Age: 18 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Intervention/Treatment |
| --- |
|Drug: Rose hips extract + blueberry/blackberry extract + resveravine|nan|
|Drug: Rose hips extract + blueberry/blackberry extract + Aframomum melegueta extract|nan|
|Drug: Rose hips extract + resveravine + Aframomum melegueta extract|nan|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| - Serum C-reactive protein levels |  |  | 
| - In vivo IL-1 gene expression |  |  | 
| - Ex vivo IL-1 production |  |  | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| - Cytokine Inflammatory Panel (IL-1-alpha, TNF-alpha, IL-6, IL-8, IL-10, and IL-12) |  |  | 
| - Serum Amyloid A (SAA) |  |  | 
| - Serum Intracellular Adhesion Molecule-1 (ICAM-1) |  |  | 
| - Fibrinogen |  |  | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
Inflammation, Interleukin-1, C-reactive protein, Genotype, Rose Hips, Blueberry, Blackberry, Aframomum melegueta, Resveravine