Comparison of Acute Tart Cherry Supplement Formulation and Dose on Inflammation and Oxidative Capacity

Study Overview
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Brief Summary
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A comparison of acute tart cherry formations (juice vs. powdered) and doses (single vs. twice daily) on inflammation and oxidative capacity.

Detailed Description
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Participants will be randomly allocated to a group: one tart cherry capsule daily, two tart cherry capsules daily, one 8oz. bottle of tart cherry juice daily, two 8oz. bottles of tart cherry juice taken 8 hours apart, one placebo pill, and one 8oz. bottle of placebo. Participants will arrive to the lab at 8 am after an overnight fast of 10 hours. They will provide a blood sample and then ingest their study treatment. Participants will stay in the lab for a further 2 hours for additional blood draws then return to the lab in 6 hours for another blood draw. At this time if they are in a twice daily group they will receive their second treatment for the day. All participants will come back to the lab 24 hours later where they will give a blood sample and receive their next treatment. If they are in the twice daily group they will be provided with their next dose and given instructions to consume it in 8 hours. Participants will arrive 24 hours later for a final blood draw with no supplementation. Blood samples will be measured pre-ingestion, 1 hour, 2 hours, 8 hours, 24 hours and 48 hours post-ingestion. Samples will be analyzed for plasma oxygen radical absorbance capacity (ORAC), uric acid (UA), and C-reactive protein (CRP).

Official Title
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Comparison of Acute Tart Cherry Supplement Formulation and Dose on Inflammation and Oxidative Capacity

Conditions
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Inflammation, Oxidative Stress

Intervention / Treatment
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* Dietary Supplement: tart cherry
* Dietary Supplement: placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: free of cardiovascular, metabolic and inflammatory conditions non-smokers no known allergy to cherries or cherry juice not be taking food supplements (turmeric/curcumin, cherry products, greens, etc.). Exclusion Criteria: quit smoking less than one year ago. currently being treated for arthritis or an inflammatory condition. currently being treated for uncontrolled cardiovascular disease, high blood pressure, diabetes, fibromyalgia, or irritable bowel syndrome. pregnant. regularly consume cherries or are allergic to cherries or dairy. currently use anti-inflammatory medications, have used corticosteroids in the last two months.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: single dose tart cherry capsule<br>single dose tart cherry capsule | Dietary Supplement: tart cherry<br>* Either tart cherry juice or freeze dried powdered tart cherry in capsule was given<br>|
| Experimental: double dose tart cherry capsule<br>double dose tart cherry capsule | Dietary Supplement: tart cherry<br>* Either tart cherry juice or freeze dried powdered tart cherry in capsule was given<br>|
| Experimental: single dose tart cherry juice<br>single dose tart cherry juice | Dietary Supplement: tart cherry<br>* Either tart cherry juice or freeze dried powdered tart cherry in capsule was given<br>|
| Experimental: double dose tart cherry juice<br>double dose tart cherry juice | Dietary Supplement: tart cherry<br>* Either tart cherry juice or freeze dried powdered tart cherry in capsule was given<br>|
| Experimental: single placebo capsule<br>single placebo capsule | Dietary Supplement: placebo<br>* either cornstarch capsule or kool-aid<br>|
| Experimental: single placebo juice<br>single placebo juice | Dietary Supplement: placebo<br>* either cornstarch capsule or kool-aid<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in c-reactive protein | c-reactive protein (mg/L) measured via assay | 1 hour, 2 hours, 8 hours, 24 hours, and 48 hours post ingestion | 
| Change in uric acid | uric acid (mg/dL) measured via assay | 1 hour, 2 hours, 8 hours, 24 hours, and 48 hours post ingestion | 
| Change in oxidative capacity | oxygen radical absorbance capacity (uM Trolox equivalents) measured via assay | 1 hour, 2 hours, 8 hours, 24 hours, and 48 hours post ingestion |