Z-Endoxifen Hydrochloride in Treating Patients With Metastatic or Locally Recurrent Estrogen Receptor-Positive Breast Cancer

Study Overview
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Brief Summary
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This phase I trial studies the side effects and the best dose of Z-endoxifen hydrochloride in treating patients with estrogen receptor-positive (ER+) breast cancer that has spread to other places in the body (metastatic) or has come back at or near the same place as the original tumor (locally recurrent). Estrogen can cause the growth of breast cancer cells. Hormone therapy using Z-endoxifen hydrochloride may fight breast cancer by blocking the use of estrogen by tumor cells.

Detailed Description
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PRIMARY OBJECTIVES: l. To determine either the maximum-tolerated dose (MTD) of Z-endoxifen hydrochloride or the dose level associated with endoxifen steady state concentration (Css) of at least 2 uM in women with metastatic estrogen-receptor positive (ER+) breast cancer. (Dose Escalation Cohort) II. To describe the safety profile of Z-endoxifen (Z-endoxifen hydrochloride) at each of the doses examined. (Dose Escalation Cohort) III. To evaluate changes in vision after 2 cycles of treatment. (Dose Escalation Cohort) IV. To gather preliminary data on the clinical benefit in terms of tumor response rate and progression-free survival. (Dose Escalation Cohort) V. To evaluate the changes in the frequency and severity of hot flashes after 2 cycles of treatment. (Expansion Cohort) VI. Evaluate changes in irritability scale using a validated irritability questionnaire. (Expansion Cohort) VII. To evaluate changes in markers of bone formation and absorption after 2 cycles of treatment. (Expansion Cohort) XIII. To evaluate changes in vision after 2 cycles of treatment. (Expansion Cohort). IX. To further characterize the safety profile of Z-endoxifen. (Expansion Cohort) SECONDARY OBJECTIVES: I. To characterize the plasma pharmacokinetics and urinary excretion of Z-endoxifen at each of the doses examined. II. For patients beginning in dose level 7 as well as the expansion cohorts, we will describe any changes in tumor expression of ER (both full length and truncated forms), progesterone receptor (PR), steroid receptor co-activator (SRC)1, SRC3, as well as the insulin-like growth factor receptor (IGF)1R/phosphatidylinositol 3 kinase (PI3K)/v-akt Murine Thymoma Viral Oncogene Homolog 1 (AKT)/mammalian target of rapamycin (mTOR) pathway and proliferation-related Ki-67 antigen (Ki67) after 1 cycle of treatment (approximately 28 days). III. To determine the frequency of estrogen receptor 1 (ESR1) mutations and the presence of antitumor activity (response rate and progression free survival [PFS]) in all patients whose tumors harbor ESR1 alterations. IV. To determine whether the ESR1 mutations identified in pre-treatment tumor biopsies can be detected in matched plasma cell free deoxyribonucleic acid (DNA) from the same patients. OUTLINE: This is a dose-escalation study followed by an expansion cohort study. Patients receive Z-endoxifen hydrochloride orally (PO) on days 1-28. Courses repeat every 28 days in the absence of disease progression or unacceptable toxicity. After completion of study treatment, patients are followed up for 30 days and then at 3 months.

Official Title
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Phase I Study of Z-Endoxifen as a Hormonal Therapy for Breast Cancer

Conditions
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HER2/Neu Positive, Recurrent Breast Carcinoma, Stage IIIC Breast Cancer AJCC v7, Stage IV Breast Cancer AJCC v6 and v7

Intervention / Treatment
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* Drug: Endoxifen Hydrochloride
* Other: Laboratory Biomarker Analysis
* Other: Pharmacological Study
* Other: Questionnaire Administration


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Histologically confirmed diagnosis of metastatic or locally recurrent breast cancer ER positive defined as > 1% nuclear staining on the biopsy that was obtained at the confirmation of metastatic or locally recurrent disease Lesion type of either evaluable or measurable disease Pre- or post-menopausal female For the expansion cohorts: tumor that is accessible for biopsy Eastern Cooperative Oncology Group (ECOG) performance status (PS) 0 or 1 Life expectancy > 16 weeks Capable of understanding investigational nature, potential risks and benefits of the study and able to provide written informed consent Absolute neutrophil count (ANC) >= 1,000/uL Platelet count >= 75,000/uL Total bilirubin =< 1.5 x institutional upper limit of normal (ULN) Aspartate aminotransferase (AST) (serum glutamic oxaloacetic transaminase [SGOT]) and alanine aminotransferase (ALT) (serum glutamate pyruvate transaminase [SGPT]) =< 2.5 x institutional ULN (< 5 x institutional ULN if liver function test [LFT] elevations due to liver metastases) Creatinine =< 1.5 x institutional ULN Women with human epidermal growth factor (HER)-2 positive disease must have received and progressed on at least one prior anti-HER-2 directed regimen (trastuzumab, lapatinib) for their metastatic disease For dose escalation cohort: Any number of prior systematic therapy regimens is allowed NOTE: prior systematic therapy in the adjuvant setting is not required At least one prior hormone containing regimen in the metastatic setting (tamoxifen if pre-menopausal; aromatase inhibitor if post-menopausal) NOTE: exception: patients that fail (defined as the development of metastatic disease while receiving an adjuvant hormone containing regimen of tamoxifen for premenopausal and aromatase inhibitor for postmenopausal) are eligible and not required to receive additional hormonal containing regimens prior to enrollment At least one prior chemotherapy containing regimen in adjuvant and/or metastatic setting For the expansion cohort(s): At least one prior hormone containing regimen in the metastatic setting (tamoxifen if pre-menopausal; aromatase inhibitor if post-menopausal) NOTE: exception: patients that fail (defined as the development of metastatic disease while receiving an adjuvant hormone containing regimen of tamoxifen for premenopausal and aromatase inhibitor for postmenopausal) are eligible and not required to receive additional hormonal containing regimens prior to enrollment NOTE: a prior hormone containing regimen in the adjuvant setting is not required; a hormonal regimen containing everolimus is allowed Either 1 or 2 prior chemotherapy regimens are allowed but not required such that both are in the metastatic setting or one is in the adjuvant setting and one in the metastatic setting (note, an anthracycline and taxane based regimen delivered in the adjuvant setting would be considered one regimen) Willingness to return to Mayo Clinic Rochester, Arizona, or Florida during treatment phase of the trial Dose Escalation cohort only: Mandatory Translational Research Components Willingness to provide biologic specimens (blood and urine) Dose Escalation cohorts beginning at 160 mg/day: Mandatory Translational Research Components Willingness to provide biologic specimens (tissue) Dose Expansion cohort(s): Mandatory Translational Research Components Willingness to provide biologic specimens (blood, tissue and urine) Note: The goals of this study include assessment of the biologic effects on surrogate markers of Z-endoxifen and therefore, are contingent upon availability of the biologic specimens Women of childbearing potential only: negative serum pregnancy test done =< 48 hours prior to registration Capable of swallowing 20-mg capsules Exclusion Criteria: Any of the following therapies prior to registration: Chemotherapy =< 3 weeks Immunotherapy =< 3 weeks Biologic therapy =< 3 weeks Hormonal therapy =< 3 weeks Monoclonal antibodies =< 3 weeks Radiation therapy =< 3 weeks Anti-Her-2 directed therapy =< 3 weeks Prior endoxifen therapy Prior history of: Stroke =< 6 months prior to registration Seizures =< 3 months prior to registration Deep vein thrombosis (DVT) or pulmonary embolism (PE) =< 12 months prior to registration Two or more episodes of DVT and/or PE =< 5 years prior to registration Crystalline retinopathy Abnormal uterine bleeding =< 1 year prior to registration Personal history of coagulopathy Active DVT and/or PE requiring anti-coagulant therapy Patients who are on anti-coagulant therapy for maintenance are eligible as long as the DVT and/or PE was > 12 months prior to enrollment and there is no evidence for active thrombosis (either DVT or PE) Clinically symptomatic cataracts requiring imminent surgery Note: patients that have cataracts that do not require surgery are eligible Other invasive malignancy that has been diagnosed or has recurred < 2 years prior to registration; EXCEPTIONS: non-melanotic skin cancer or carcinoma-in-situ of the cervix Any co-morbid systemic illnesses or other severe concurrent disease which, in the judgment of the investigator, would make the patient inappropriate for entry into this study or interfere significantly with the proper assessment of safety and toxicity of the prescribed regimens Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, hypertension, or psychiatric illness/social situations that would limit compliance with study requirements Failure to fully recover from acute, reversible effects of prior chemotherapy regardless of interval since last treatment; EXCEPTION: neuropathies - if grade 2 neuropathies have been stable for at least 3 months since completion of prior treatment patient is eligible Tumors involving the spinal cord or heart Uncontrolled brain metastases Note: brain metastases are not permitted on study unless the metastases have been treated by surgery or radiotherapy, and the patient has been neurologically stable and off steroids for >= 12 weeks Plans to begin bisphosphonates or denosumab after registration or began a bisphosphonate or denosumab regimen < 90 days before registration Note: patients on a stable dose of bisphosphonates or denosumab for > 90 days prior to registration are eligible Any of the following: Pregnant women Nursing women Women of childbearing potential who are unwilling to employ adequate contraception Other concurrent chemotherapy or anti HER2 therapy, immunotherapy, radiotherapy, or any ancillary therapy considered investigational (utilized for a non-Food and Drug Administration [FDA]-approved indication and in the context of a research investigation)

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Treatment (Z-endoxifen hydrochloride)<br>Patients receive Z-endoxifen hydrochloride PO on days 1-28. Courses repeat every 28 days in the absence of disease progression or unacceptable toxicity. | Drug: Endoxifen Hydrochloride<br>* Given PO<br>* Other names: Z-Endoxifen Hydrochloride;Other: Laboratory Biomarker Analysis<br>* Correlative studies<br>Other: Pharmacological Study<br>* Correlative studies<br>Other: Questionnaire Administration<br>* Ancillary studies<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| MTD defined as the highest dose level where at most 1 of 6 patients develops a dose limiting toxicity and 2 or more of the 3-6 patients treated at the next higher dose level develop a dose limiting toxicity | Assessed using the National Cancer Institute Common Terminology Criteria for Adverse Events version 4.0. The maximum grade of each type of toxicity will be recorded for each patient. For each toxicity reported by dose level, the percentage of patients developing any degree of that toxicity as well as the percentage of patients developing a severe degree (grade 3 or higher) will be determined. | 28 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Progression-free survival |  | From study entry to the documentation of disease progression, assessed up to 3 months | 
| Overall survival |  | From study entry to death due to any cause, assessed up to 3 months | 
| Change in hot flash scores graded using a hot flash diary and the hot flash interference scale | The number and severity of hot flashes will be examined. Times series plots of hot flash scores will be constructed to visually assess changes across time within a dose level and between dose levels. In particular, for each expansion dose level, a 90% confidence interval will be constructed for the proportion of patients whose hot flash scores increase at least 2 fold from baseline after one course of treatment. | Baseline to day 28 |