Surface Roughness of a Dental Restorative Material and Biofilm Formation

Study Overview
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Brief Summary
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In this study we intend to find out the pattern of biofilm accumulation in vivo on various surface finishings. Our intention is to prepare Polymethylmetacrylate squers on orthodontical brackets,then prepare the surface of the sample and measure the surface roughness with a profilimeter and get the mean value in Ra units, then place the brackets on lower premolars and molars for 12 hours, remove them and examine the biofilm formed with confocal scanning microscope.

Detailed Description
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Several studies in the past to assess the effects of surface roughness on the amount of plaque accumulation in vitro, whereas only few performed a modern in vitro assessment of the plaque accumulation. In this study we intend to find out the pattern of biofilm accumulation in vivo on various surface finishings. Our intention is to prepare Polymethylmetacrylate squers on orthodontical brackets,then prepare the surface of the sample and measure the surface roughness with a profilimeter and get the mean value in Ra units, then place the brackets on lower premolars and molars for 12 hours, remove them and examine the biofilm formed with confocal scanning microscope. Statistical evaluation of pilot study determined the need to perform this research on twelve(12) candidates. It is our intention to check two materials in each candidate: composite resin and polymethylmetacrylate.

Official Title
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In Vivo Study of the Relationship Between Surface Roughness of a Dental Restorative Material and Biofilm Formation

Conditions
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Diabetic, Antibiotic Intake

Intervention / Treatment
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* Procedure: dental restorative material surface preparation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: any patient with intact buccal surfaces of lower molars and premolars Exclusion Criteria: patients that used antibiotics in the three months prior to the experiment

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: Single


Arms and Interventions

| Intervention/Treatment |
| --- |
|Procedure: dental restorative material surface preparation|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| confocal scanning laser microscope evaluation of biofilm formation |  |  | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| roughness analysis of the samples with profilometer |  |  | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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biofilm formation, dental plaque, surface roughness, dental restoration, dental materials