Influence of Protein Hydrolysis on Dietary Protein Digestibility and Metabolism in Healthy Subjects

Study Overview
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Brief Summary
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Hydrolysis of dietary protein may impact their gastrointestinal kinetics and further metabolism. The primary goal of this work was to measure the metabolic fate of intact or hydrolyzed protein using a protein of good nutritional quality, i.e., casein. A second aim of this study was to assess the true ileal digestibility of dietary protein using different methods including the hydrolyzed casein method and to measure its possible effect on endogenous intestinal N losses in humans.

Official Title
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Influence of Protein Hydrolysis on Dietary Protein Digestibility and Metabolism in Healthy Subjects

Conditions
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Healthy

Intervention / Treatment
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* Other: Intestinal and metabolic exploration
* Dietary Supplement: 15N-labelled intact casein
* Dietary Supplement: 15N-labelled hydrolyzed casein
* Dietary Supplement: mixture of AA


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy subjects BMI between 18 and 25 Exclusion Criteria: allergy to dairy proteins positive serology to AgHbS, HCV or HIV pregnancy abusive drug or alcohol consumption.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Parallel Assignment


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Intact casein<br>Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled intact casein | Other: Intestinal and metabolic exploration<br>* Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.<br>Dietary Supplement: 15N-labelled intact casein<br>* standard mixed meal containing 15% of energy as 15N-labelled intact casein<br>|
| Experimental: Hydrolyzed casein<br>Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein | Other: Intestinal and metabolic exploration<br>* Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.<br>Dietary Supplement: 15N-labelled hydrolyzed casein<br>* standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein<br>|
| Experimental: AA<br>Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine | Other: Intestinal and metabolic exploration<br>* Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.<br>Dietary Supplement: mixture of AA<br>* standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Postprandial metabolic fate of dietary N |  | 0h-1h-2h-3h-4h-5h-6h-7h-8h postprandial | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Differences in ileal digestibility determination according to the method used |  | 8h postprandial |