Effects of Anesthetic Methods on Hepatic Cancer Cell Malignancy

Study Overview
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Brief Summary
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Numerous studies found that anesthetic methods can influence the recurrence of tumor and the overall survival of patients after primary cancer surgery. Radiofrequency (RF) ablation is now widely used in clinic for treatment of hepatocellular carcinoma (HCC). Currently, diverse anesthetic methods, including general anesthesia (GA), epidural anesthesia and local anesthesia (LA), are used for RF ablation surgery. Using serum from HCC surgery patients randomized to receive either GA or LA during surgery, we investigated the effects of anesthetic methods on proliferation, migration and metastasis in HepG2 hepatic cancer cells in vitro.

Official Title
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Effects of Serum From Hepatocellular Carcinoma Surgery Patients Under Different Anesthetic Methods on Hepatic Cancer Cell Malignancy in Vitro

Conditions
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Hepatocellular Carcinoma

Intervention / Treatment
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* Procedure: anesthetic methods


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: patients with a single primary liver tumor of 3cm or smaller, who are scheduled for RF ablation surgery Exclusion Criteria: less than 18 or more than 65 years old; ASA Physical Status 4 or greater; previous surgery in liver (including radiofrequency ablation); severe systemic disease (heart, lung, kidney, or immune system); INR>1.5 or platelet count <45,000 cells/mm3; a history of addiction to opioids; with known extension beyond the liver; Child-Pugh Class C.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: general anesthesia<br>Patients in this group will have RF ablation for treatment of HCC under general anesthesia. | Procedure: anesthetic methods<br>* Patients will receive standard GA or LA for RF surgery.<br>|
| Active Comparator: local anesthesia<br>Patients in this group will have RF ablation for treatment of HCC under local anesthesia. | Procedure: anesthetic methods<br>* Patients will receive standard GA or LA for RF surgery.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| cell proliferation | HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA for 24h and cell proliferation will be measured | 1 hour post-surgery | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| cell migration | HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell migration will be measured | 1 hour post-surgery | 
| cell metastasis | HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell metastasis will be measured | 1 hour post-surgery |