Protein Nutrition for Muscle Hypertrophy in Older Adults

Study Overview
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Brief Summary
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The primary objective is to determine the effect of reduced activity on the stimulation of myofibrillar FSR with protein feeding in the elderly.

Detailed Description
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The primary objective is to determine the effect of reduced activity on the stimulation of myofibrillar FSR with protein feeding in the elderly

Official Title
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Efficacy of Isolated Whey and Micellar Whey Protein (+/- Citrulline) to Stimulate Protein Synthesis in 'Healthy' and 'Anabolically Resistant' Elderly Muscle

Conditions
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Healthy Elderly Muscle, Anabolically Resistant Elderly Muscle

Intervention / Treatment
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* Dietary Supplement: isolated whey protein
* Dietary Supplement: micellar whey protein
* Dietary Supplement: glycine
* Dietary Supplement: citrulline


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Must habitually complete a minimum of 5000 steps per day Having obtained informed consent Non-obese Non-smoker Exclusion Criteria: Aged >80 years Inability to comply with study procedures Currently participating or having participated in another infusion trial during last 12 months Any acute or chronic llness History of neuromuscular problems Medications known to affect protein metabolism Individuals who complete fewer than 5000 steps per day Individuals who have metal implants

Ages Eligible for Study
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Minimum Age: 65 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: 20g isolated whey protein + 30g carb, 8,5g fat, 15g glycine<br>20g isolated whey protein + 30g carb, 8,5g fat, 15g glycine | Dietary Supplement: isolated whey protein<br>* isolated whey protein<br>Dietary Supplement: glycine<br>* glycine<br>|
| Experimental: 20g micellar whey protein + 30g carb, 8.5g fat, 15g glycine<br>20g micellar whey protein + 30g carb, 8.5g fat, 15g glycine | Dietary Supplement: micellar whey protein<br>* micellar whey protein<br>Dietary Supplement: glycine<br>* glycine<br>|
| Experimental: 20g micellar whey protein + 30g carb, 8.5g fat, 5g citrulline<br>20g micellar whey protein + 30g carb, 8.5g fat, 5g citrulline | Dietary Supplement: micellar whey protein<br>* micellar whey protein<br>Dietary Supplement: citrulline<br>* citrulline<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in myofibrillar fractional synthetic rate (FSR) |  | one day and 2 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in non-myofibrillar fractional synthetic rate (FSR) |  | baseline and one day of feeding | 
| Capillary dilatation and blood flow | Determine if inactivity reduces the feeding-induced increase in capillary dilation and nutrient delivery and whether citrulline rescues this in resistant muscle or augments it in the healthy muscle as measured by Definity infusion and contrast enhanced ultrasound (CEUS) | 2 weeks |