Study of Cytokines Serum Levels in Chronic Low Back Pain

Study Overview
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Brief Summary
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The purpose of this study is to: evaluate if there is an association between elevated levels of cytokines and chronic pain due to herniated disk disease measured cytokines levels in chronic low back pain and in healthy subjects.

Detailed Description
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23 patients with at least three months of back pain due to herniated disk disease were selected Diagnosis was confirmed by magnetic resonance imaging (MRI) or computerized tomography (CT) imaging of the spine for all patients. In addition, pain severity had to be ³ 5 points on the Numerical Rating Scale (NRS) ranging from zero (no pain) to 10 (worst imaginable pain). The exclusion criteria were defined as one or more of the following: psychiatric disorders, systemic or inflammatory disease, history of allergy, presence of motor deficits, history of blood dyscrasia, pregnancy, active infection, tumor, use of analgesic drugs in the week before or inability to come to the hospital for evaluation.

Official Title
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Analytical Transversal Study of Cytokines Serum Levels in Chronic Low Back Pain Due to Herniated Disk

Conditions
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Interleukines

Intervention / Treatment
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* Other: blood collect


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 23 patients least three months of back pain due to herniated disk disease pain severity had to be 5 points on the Numerical Rating Scale (NRS) 10 healthy volunteers: without previous history of back pain Exclusion Criteria: One or more of the following: psychiatric disorders systemic or inflammatory disease history of allergy presence of motor deficits history of blood dyscrasia pregnancy active infection, tumor use of analgesic drugs in the week before inability to come to the hospital for evaluation.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Health Services Research
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: cytokine levels<br>Serum levels of pro-inflammatory cytokines were measured using the Enzyme Linked Immuno Sorbent Assay (ELISA) technique in 23 patients with pain due to herniated disk disease (G1) as well as in 10 control healthy subject | Other: blood collect<br>* Five milliliters of venous blood was drawn in the morning from subjects and immediately centrifuged.<br>* Other names: serum levels;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| cytokines levels in patients with chronic low back pain and in healthy subjects |  | 30 days after collect | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Cytokines, Low back pain, Intervertebral disk displacement, Interleukins, Tumor necrosis factor-alpha