Immunologic Biomarker Profile of Cerebrospinal Fluid

Study Overview
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Brief Summary
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The aim of this study was the describe the Immunologic biomarker profile of cerebrospinal fluid in multiple sclerosis patients

Official Title
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Immunologic Biomarker Profile of Cerebrospinal Fluid

Conditions
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Multiple Sclerosis, Neuromyelitis Optica

Intervention / Treatment
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* Other: Biomarker analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: patients who have had neuromyelitis ptica with anti-AQP4 antibodies, or anti- Myelin Oligodendrocyte Glycoprotein antibodies, or double seronegative antibodies. Neuromyelitis Optica patients have been prospectively included in the OFSEP collection Multiple sclerosis patients, isolated myelitis or optic neuritis patients, and symptomatic controls whose samples will come from the NEURO collection of the Hospital Biological Collections Center, Patients have given their free consent for use of their samples in research
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Neuromyelitis optica with anti-MOG<br> | Other: Biomarker analysis<br>* Detection of biomarker in cerebrospinal fluid<br>|
| Patients with Neuromyelitis optica with anti-AQP4<br> | Other: Biomarker analysis<br>* Detection of biomarker in cerebrospinal fluid<br>|
| Seronegative patients with Neuromyelitis optica<br> | Other: Biomarker analysis<br>* Detection of biomarker in cerebrospinal fluid<br>|
| Patients with recurrent-remitting multiple sclerosis<br>Patients with recurrent-remitting multiple sclerosis with medullary or optic involvement | Other: Biomarker analysis<br>* Detection of biomarker in cerebrospinal fluid<br>|
| Progressive multiple sclerosis patients<br> | Other: Biomarker analysis<br>* Detection of biomarker in cerebrospinal fluid<br>|
| Symptomatic controls<br> | Other: Biomarker analysis<br>* Detection of biomarker in cerebrospinal fluid<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Level of glial fibrillar acidic protein in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of chitinase-3-like protein 1 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of neurofilament in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin-6 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin-8 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of C-X-C motif ligand (CXCL-13) in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of Matrix metalloproteinase-9 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of Soluble CD27 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin 10 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin 17A in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin 1B in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of monocyte chemoattractant protein-1 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of soluble intercellular adhesion molecule-1 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 
| Level of soluble vascular cell adhesion molecule-1 in cerebrospinal fluid in patients with Neuromyelitis Optica with anti- Myelin Oligodendrocyte Glycoprotein | enzyme-linked immunosorbent assay | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Level of glial fibrillar acidic protein in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of chitinase-3-like protein 1 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of neurofilament in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin-6 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin-8 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of C-X-C motif ligand (CXCL-13) in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of Matrix metalloproteinase-9 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of Soluble CD27 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin 10 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin 17A in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of Interleukin 1B in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of monocyte chemoattractant protein-1 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of soluble intercellular adhesion molecule-1 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline | 
| Level of soluble vascular cell adhesion molecule-1 in cerebrospinal fluid in each group | enzyme-linked immunosorbent assay | Baseline |