Prolonged Sitting on Responses to Short-Term Exercise Training

Study Overview
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Brief Summary
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Prolonged sitting has been shown to effect the response to a high fat tolerance test (HFTT) after acute exercise. This study will evaluate the effect of prolonged sitting on response to a HFTT after a short term training period.

Official Title
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Effect of Prolonged Sitting on Metabolic and Cardiovascular Responses to Short-Term Exercise Training

Conditions
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Atherosclerosis, Metabolic Syndrome

Intervention / Treatment
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* Behavioral: Prolonged sitting with exercise
* Behavioral: Active walking with exercise.


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 to 35 years Exclusion Criteria: cardiovascular problems (e.g. pre-existing heart issues, coronary artery disease, hypertension, etc.) respiratory problems musculoskeletal problems that prevent prolonged sitting or exercise obesity susceptibility to fainting pregnancy

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: Subjects will be randomly assigned to either the sitting plus exercise or walking plus exercise.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Sitting with Exercise<br>Subjects will complete a short term training protocol while sitting >11 hours per day. This training will be preceded by a VO2max test to aid in setting the correct intensity for training. Subjects will be asked to complete three high fat tolerance tests. One to establish a baseline, the second to determine the acute effects of training, and a third at the completion of training to determine the cumulative effect of exercise training. | Behavioral: Prolonged sitting with exercise<br>* Subjects will commit to sitting more than 11 hours per day and taking less than 5000 steps per day while participating in exercise training.<br>|
| Active Comparator: Walking with exercise<br>Subjects will complete a short term training protocol while sitting <5 hours per day. This training will be preceded by a VO2max test to aid in setting the correct intensity for training. Subjects will be asked to complete three high fat tolerance tests. One to establish a baseline, the second to determine the acute effects of training, and a third at the completion of training to determine the cumulative effect of exercise training. | Behavioral: Active walking with exercise.<br>* Subjects will commit to sitting less than 5 hours per day and taking more than 10000 steps per day while participating in exercise training.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma Triglycerides | Areas under the curve for triglyceride concentration will be calculated for hourly samples from a 6-hour lipid tolerance test | 6 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma Insulin | Areas under the curve for insulin concentration will be calculated for hourly samples from a 6-hour lipid tolerance test | 6 hours | 
| Plasma glucose | Areas under the curve for glucose concentration will be calculated for hourly samples from a 6-hour lipid tolerance test | 6 hours |