Effect of Daily Steps on Fat Metabolism

Study Overview
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Brief Summary
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Reduced ambulatory activity has been shown to effect the response to a high fat tolerance test (HFTT) after acute exercise. This study will evaluate the effect of varying levels of daily ambulation on response to a HFTT after an acute aerobic exercise bout.

Official Title
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Effect of Daily Ambulatory Activity on the Responses to Acute Aerobic Exercise.

Conditions
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Atherosclerosis, Metabolic Syndrome

Intervention / Treatment
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* Behavioral: Very Low Steps
* Behavioral: Low Steps
* Behavioral: Moderate Steps


Participation Criteria
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Eligibility Criteria
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Exclusion Criteria: Cardiovascular problems (e.g. pre-existing heart issues, coronary artery disease, hypertension, etc.) Respiratory problems Musculoskeletal problems that prevent prolonged sitting or exercise Obesity Susceptibility to fainting Pregnancy

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Subjects will complete three experimental conditions which include 2 days of reduced step count (2,500, 5,000, and 7,500 steps/day) with 1-hour of exercise at 65% VO2max.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Very Low Steps<br>Subjects will be asked to undergo reduced daily stepping to a level of 2,500 steps/d for 2 days. On the evening of day 2, they will be asked to run at 65% of VO2max for 1-hour. | Behavioral: Very Low Steps<br>* Subjects will have a 2-day control period during which step count and diet will be controlled for and recreated for the other trials. Following this 2-day period, they will undergo the Very Low Stepping trial with the acute bout of exercise on day 2. Day 3 will consist of the lipid tolerance test to determine the ability of the body to clear triglycerides.<br>|
| Experimental: Low Steps<br>Subjects will be asked to undergo reduced daily stepping to a level of 5,000 steps/d for 2 days. On the evening of day 2, they will be asked to run at 65% of VO2max for 1-hour. | Behavioral: Low Steps<br>* Subjects will have a 2-day control period during which step count and diet will be controlled for and recreated for the other trials. Following this 2-day period, they will undergo the Low Stepping trial with the acute bout of exercise on day 2. Day 3 will consist of the lipid tolerance test to determine the ability of the body to clear triglycerides.<br>|
| Experimental: Moderate Steps<br>Subjects will be asked to undergo reduced daily stepping to a level of 7,500 steps/d for 2 days. On the evening of day 2, they will be asked to run at 65% of VO2max for 1-hour. | Behavioral: Moderate Steps<br>* Subjects will have a 2-day control period during which step count and diet will be controlled for and recreated for the other trials. Following this 2-day period, they will undergo the Moderate Stepping trial with the acute bout of exercise on day 2. Day 3 will consist of the lipid tolerance test to determine the ability of the body to clear triglycerides.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 6-Hour Plasma Triglyceride Area Under the Curve | Areas under the curve for triglyceride concentration will be calculated for hourly samples from a 6-hour lipid tolerance test. | 6-hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 6-Hour Plasma Insulin Area Under the Curve | Areas under the curve for insulin concentration will be calculated for hourly samples from a 6-hour lipid tolerance test | 6-hours | 
| 6-Hour Plasma Glucose Area Under the Curve | Areas under the curve for glucose concentration will be calculated for hourly samples from a 6-hour lipid tolerance test. | 6-hours |