Effect of Muscle Damage After Downhill Running on Postprandial Lipids

Study Overview
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Brief Summary
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All participants underwent three experimental trials, namely downhill running (DR), level running (LR), and the control (CON) in a cross-over design. Each trial lasted for 2 days. The participants were fed the same breakfast and lunch on day 1 in each trial. The participants arrived at the laboratory at 18:00 on day 1 and ran on the treadmill at 60% VO2max (downhill and level surface trials) or rested (CON trial) for 30 min. Subsequently, they were fed a standardized dinner (A meal box containing chicken, vegetables, and rice, 692 kcal, with 50% energy from carbohydrate, 32% from fat, and 18% from protein) within 20 min. The participants returned to the laboratory at 07:30 on day 2 after an overnight fast. After baseline blood and gas samples were collected, the participants were fed a high-fat meal that included cereal, white bread, whipping cream, cheese, and butter. The high-fat meal provided fat 1.2 g/kg (65% energy), carbohydrate 1.1 g/kg (27% energy), protein 0.33 g/kg (8% energy), and 16.5 kcal/kg. A 10-mL blood sample was collected from a forearm vein into nonheparinized tubes before and immediately after exercise or rest on day 1. On day 2, postprandial blood samples were collected from forearm veins into nonheparinized tubes by using an indwelling venous needle and a three-way stopcock. A 10-mL blood sample was collected before (0 h) and 0.5, 1, 2, 3, 4, 5, and 6 h after the high-fat meal.

Official Title
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Effect of Muscle Damage After Downhill Running on Postprandial Lipids

Conditions
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Nutritional and Metabolic Diseases, Lipemia

Intervention / Treatment
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* Behavioral: Exercise
* Behavioral: Rest


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: generally healthy, had not engaged in regular exercise or received any medication in the past 6 months Exclusion Criteria: history of cardiovascular disease, hypertension, hyperlipidemia, diabetes, atherosclerosis, or osteoporosis, free of any musculoskeletal injury during the study

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Downhill running<br>Running at 60% VO2max on -15% slope for 30 min on a treadmill | Behavioral: Exercise<br>* Running<br>|
| Experimental: Level running<br>Running at 60% VO2max on level surface for 30 min on a treadmill | Behavioral: Exercise<br>* Running<br>|
| Placebo Comparator: Control<br>Rest 30 min | Behavioral: Rest<br>* sit and rest<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| TG AUC | area under the curve for serum triglyceride concentrations within 6 hours after the high-fat meal | 6 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| NEFA AUC | area under the curve for serum non-esterified fatty acid concentrations within 6 hours after the high-fat meal | 6 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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triglyceride, non-esterified fatty acids, eccentric exercise, cardiovascular disease