Human Gut Microbiota and Prebiotic Response

Study Overview
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Brief Summary
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This study is designed to examine the shift in gut microbiota and their metabolism after consumption of a dietary supplement (prebiotic) by healthy participants. The investigators will provide participants with a panel of over the counter prebiotics and monitor their gut microbiome response in stool. All prebiotics will be commercially available, over-the-counter food, food additives, or dietary supplements, without any known market-use adverse effects beyond minimal discomfort.

Official Title
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Human Gut Microbiota and Prebiotic Response

Conditions
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Healthy Gut Microbiota

Intervention / Treatment
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* Dietary Supplement: Prebiotic A - inulin
* Dietary Supplement: Prebiotic B - galactooligosaccharides(GOS)
* Dietary Supplement: Prebiotic C - wheat dextrin


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Provide Stool Samples at no risk to yourself Exclusion Criteria: No oral antibiotic treatments within previous 1 month No known allergy to milk products or lactose intolerance no known allergy to wheat or gluten intolerance no history of irritable bowel syndrome no history of inflammatory bowel disease no history of type-2 diabetes no history of chronic kidney disease or reduced kidney function no current intestinal obstructions no current untreated colorectal cancer not currently pregnant or breastfeeding

Ages Eligible for Study
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Minimum Age: 17 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Prebiotic A<br> | Dietary Supplement: Prebiotic A - inulin<br>* Pre-weighed packages will be given twice daily for 5 days.<br>Dietary Supplement: Prebiotic B - galactooligosaccharides(GOS)<br>* Pre-weighed packages will be given twice daily for 5 days.<br>Dietary Supplement: Prebiotic C - wheat dextrin<br>* Pre-weighed packages will be given twice daily for 5 days.<br>|
| Experimental: Prebiotic B<br> | Dietary Supplement: Prebiotic A - inulin<br>* Pre-weighed packages will be given twice daily for 5 days.<br>Dietary Supplement: Prebiotic B - galactooligosaccharides(GOS)<br>* Pre-weighed packages will be given twice daily for 5 days.<br>Dietary Supplement: Prebiotic C - wheat dextrin<br>* Pre-weighed packages will be given twice daily for 5 days.<br>|
| Experimental: Prebiotic C<br> | Dietary Supplement: Prebiotic A - inulin<br>* Pre-weighed packages will be given twice daily for 5 days.<br>Dietary Supplement: Prebiotic B - galactooligosaccharides(GOS)<br>* Pre-weighed packages will be given twice daily for 5 days.<br>Dietary Supplement: Prebiotic C - wheat dextrin<br>* Pre-weighed packages will be given twice daily for 5 days.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in levels of SCFAs in stool samples |  | Baseline and 6 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in levels of cytokines in stool samples |  | Baseline and 6 weeks |