DSM265 Prophylaxis of Plasmodium Falciparum Malaria

Study Overview
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Brief Summary
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The study is a single centre, randomised, placebo-controlled, double-blind study with DSM265 including up to two cohorts of healthy male and female volunteers aged 18 to 45 years. The study will be conducted into two sequential parts (Cohort 1 and Cohorts 2a and 2b).

Detailed Description
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Cohort 1: Controlled human malaria infection on Day 0 (Direct Venous Inoculation of 3200 cryopreserved Plasmodium falciparum sporozoites) Drug administration on Day -3 8 subjects: 6 DSM265 400 mg: 2 matching placebo Clinical safety review of Cohort 1 in order to proceed to Cohorts 2a and 2b Cohort 2a: Controlled human malaria infection on Day 0 (Direct Venous Inoculation of 3200 cryopreserved Plasmodium falciparum sporozoites) Drug administration on Day -X (to be defined following safety and pharmacokinetic data) 8 subjects: 6 DSM265 400 mg: 2 matching placebo Cohort 2b: Controlled human malaria infection on Day 0 (infected mosquito bite) Drug administration on Day -X (to be defined following safety and pharmacokinetic data) 8 subjects: 6 DSM265 400 mg: 2 matching placebo

Official Title
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Proof-of-concept, Single Center Study in Healthy Adult Volunteers to Investigate Safety, Tolerability, Pharmacokinetics and Prophylactic Activity of Single Dose DSM265 in a Controlled Human Malarial Infection Challenge Either by Direct Venous Inoculation of Plasmodium Falciparum Sporozoites or Mosquito-borne Plasmodium Falciparum

Conditions
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Healthy

Intervention / Treatment
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* Drug: DSM265
* Drug: Placebo
* Biological: Sporozoites
* Biological: Infective mosquito bite


Participation Criteria
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Eligibility Criteria
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Good health based on medical history and physical examination body mass index >18 and <30kg/m2 Laboratory results without clinically significant findings within 60 days Males must use medically acceptable form of contraception from the dose of DSM265/Placebo for 120 days Women must be of non-child bearing potential or willing and able to practice one of the required continuous acceptable methods of contraception and have negative pregnancy tests Able & willing to comply with all study requirements throughout the study Agree to undergo all study procedures, attend all study visits and stay overnight for observation if required, to last follow-up visit Willing to undergo a controlled human malaria infection by mosquito bites / inoculation Able and willing to answer all questions on informed consent correctly, and to sign the informed consent form Reachable constantly by mobile phone or email throughout the study period Refrain from blood donation throughout the study Willing to take atovaquone-proguanil, Chloroquine or other registered antimalarial Exclusion Criteria: Any history of malaria; any severe reactions to mosquito or insect bites; any history of anaphylaxis Plans to travel to malaria endemic region during the study period Previous participation in any malaria vaccine study or controlled human malaria infection study Participation in any other clinical study within 30 days of enrollment or plan to participate in another investigational vaccine/drug research during the study Breastfeeding or planning to become pregnant during the time of the study Positive Human Immunodeficiency Virus, Hepatitis B or C tests Any confirmed/suspected immunosuppressive or immunodeficient state History of serious psychiatric condition that may affect participation in the study, or history of convulsions, seizures, or of severe head trauma Symptoms, physical signs or laboratory values suggestive of systemic disorders History of cancer (except basal cell carcinoma of the skin) History of arrhythmias or documented prolonged QTcF-interval (>450 msec) or 12-lead ECG demonstrating specific abnormalities Moderate risk or higher for cardiovascular event within 5 years (>10%) Positive family history in 1st or 2nd degree relative <50 years for cardiac disease, or history of, or known active cardiac disease history of: psoriasis or porphyria which may be exacerbated by chloroquine; splenectomy, or sickle cell anaemia or other red blood cell disorders; allergy or contra-indication to chloroquine or atovaquone-proguanil Treatment with an investigational drug within 28 days or 5 half-lives of study medication; use of any drugs or dietary supplements within 7 days or 5 half-lives prior to study medication. Herbal supplements must be discontinued at least 28 days prior to study medication. As an exception, acetaminophen or ibuprofen may be used as approved by the investigator Use of any prohibited medication Grapefruit or product containing grapefruit from28 days before study medication or throughout the study History of drug or alcohol abuse within 12 months prior to dosing, or laboratory evidence of abuse Current smoking of more than 10 cigarettes or equivalent per day Plan for major surgery between enrolment and study completion

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Cohort 1 Active DSM265<br> | Drug: DSM265<br>* DSM265 400 mg single oral dose<br>Biological: Sporozoites<br>* Direct venous inoculation of 3200 cryopreserved Plasmodium falciparum (NF54 strain) sporozoites<br>* Other names: PfSPZ;|
| Placebo Comparator: Cohort 1 Placebo<br> | Drug: Placebo<br>* Placebo to match DSM265, single oral dose<br>Biological: Sporozoites<br>* Direct venous inoculation of 3200 cryopreserved Plasmodium falciparum (NF54 strain) sporozoites<br>* Other names: PfSPZ;|
| Experimental: Cohort 2a Active DSM265<br> | Drug: DSM265<br>* DSM265 400 mg single oral dose<br>Biological: Sporozoites<br>* Direct venous inoculation of 3200 cryopreserved Plasmodium falciparum (NF54 strain) sporozoites<br>* Other names: PfSPZ;|
| Placebo Comparator: Cohort 2a Placebo<br> | Drug: Placebo<br>* Placebo to match DSM265, single oral dose<br>Biological: Sporozoites<br>* Direct venous inoculation of 3200 cryopreserved Plasmodium falciparum (NF54 strain) sporozoites<br>* Other names: PfSPZ;|
| Experimental: Cohort 2b Active DSM265<br> | Drug: DSM265<br>* DSM265 400 mg single oral dose<br>Biological: Infective mosquito bite<br>* Five single bites by laboratory reared female Anopheles stephensi mosquitoes carrying Plasmodium falciparum<br>|
| Placebo Comparator: Cohort 2b Placebo<br> | Drug: Placebo<br>* Placebo to match DSM265, single oral dose<br>Biological: Infective mosquito bite<br>* Five single bites by laboratory reared female Anopheles stephensi mosquitoes carrying Plasmodium falciparum<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of subjects with positive parasitemia | positive parasitemia detected by qRT-PCR | 28 days | 
| Time interval between challenge and the first positive parasitemia | Time from infection to peripheral parasitemia (qRT-PCR) in DSM265 / placebo treated subjects in each cohort given as a geometric mean [days] | 28 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Controlled, Human, Malaria, Infection, Healthy, Volunteer