Nutrient Supplementation Influences Mitochondria Adaptation

Study Overview
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Brief Summary
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Twenty-four well trained runners (VO2max 60.73.7 ml O2 Kg-1 min1) completed a six week block randomized controlled intervention period. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO- CHO group ingested a protein beverage (0.3g kg-1) before and protein-carbohydrate beverage (0.3g protein kg-1 and 0.1g carbohydrate kg-1) after each exercise session. The CHO group ingested energy matched carbohydrate. Post-absorptive muscle biopsies were obtained pre and post intervention for analysis HAD, CS and PGC-1. Subjects completed two physical tests 6 km performance test (pre, week 1 and week 6) and aVo2max test (pre and post intervention).

Official Title
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Effect of Whey Protein Hydrolysate on Adaptions to Endurance Training in Well-trained Runners

Conditions
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Performance, Protein, Nutrition

Intervention / Treatment
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* Dietary Supplement: PROTEIN-CARBOHYDRATE
* Dietary Supplement: CARBOHDYRATE


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: maximal oxygen uptake (VO2max) of >50ml O2 kg-1 min-1 for women and >55ml O2 kg-1 min-1 for men, running at least 3 times a week and running being the primarily training form Exclusion Criteria: use of medicine diagnosed metabolic diseases injuries which hindered running and body mass index (BMI) < 25 kg m-2

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 55 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: block randomized controlled intervention
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: PROTEIN-CARBOHYDRATE<br>Intervention group receiving protein and carbohydrate supplement | Dietary Supplement: PROTEIN-CARBOHYDRATE<br>* PRO before and PRO-CHO after training<br>|
| Placebo Comparator: CARBOHYDRATE<br>Intervention groups receiving carbohydrate supplement | Dietary Supplement: CARBOHDYRATE<br>* CHO before and after training<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Maximal oxygen uptake | maximal oxygen uptake (L O2/min) | Change from week 1 to week 6 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| VDAC | Mitochondria target measured by western blotting | Change from week 1 to week 6 | 
| CytC | Mitochondria enzyme measured by western blotting | Change from week 1 to week 6 | 
| Performance | 6 km trial test | Change from week 1 to week 6 | 
| COX-IV | Mitochondria target measured by western blotting | Change from week 1 to week 6 | 
| HSP60 | Mitochondria target measured by western blotting | Change from week 1 to week 6 | 
| CD36 | Fat transporter measured by western blotting | Change from week 1 to week 6 |