IL-33, Endocan and Endothelial Cells

Study Overview
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Brief Summary
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The study aims to investigate the inflammatory response of endothelial cells to various stimulations, in particular the production of IL33 and of endocan in response to allergens, agonists of microorganisms and pollutants. For that purpose, this project attempts to set up a biological collection of lung and umbilical cord endothelial cells. Lung endothelial cells are resected from a surgical specimen, resulting from a lung cancer surgery. Umbilical cord-derived endothelial cells are taken from the umbilical cord collected during the delivery.

Official Title
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Regulation of IL33, Endocan and Their Targets in Primary Human Endothelial Cells

Conditions
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Endothelial Cell

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age > 18 years signed consent Pregnant woman for umbilical cord-derive-endothelial cells Exclusion Criteria: Age < 18 years Refusal to participate to the study Endothelial cord-drived endothelial cells : significant materno-foetal disorder (for example: meconium-stained amniotic fluid, chorioamnionitis, placental thrombosis, eclampsia etc.) infection for HIV, VHB, VHC unknown status for HIV, VHB, VHC the day of the childbirth.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Lung-derived endothelial cells<br>Every patient of more than 18 years undergoing lung cancer surgery in the department of thoracic surgery of the University Hospital of Lille. | |
| Umbilical cord-derived endothelial cells<br>Every patient of more than 18 years giving birth in the University Hospital of Lille in the absence of significant materno-foetal disorder (for example: meconium-stained amniotic fluid, chorioamnionitis, placental thrombosis, eclampsia etc.) or of infection for HIV, VHB, VHC or if unknown status for HIV, VHB, VHC the day of the childbirth. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells. |  | change from Baseline at 48 hours after stimulation | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours after stimulation | 
| change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human umbilical cord-derived endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation | 
| change in IL33 protein concentration between stimulated and unstimulated primary human lung endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation | 
| change in IL33 protein concentration between stimulated and unstimulated primary human umbilical cord-derived endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation | 
| change in the level of messenger RNA expressed as endocan / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation | 
| change in the level of messenger RNA expressed as endocan / GAPDH ratio between stimulated and unstimulated primary human umbilical cord-derived endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation | 
| change in endocan protein concentration between stimulated and unstimulated primary human lung endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation | 
| change in endocan protein concentration between stimulated and unstimulated primary human umbilical cord-derived endothelial cells. |  | change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Endothelial cells, inflammation, endothelial dysfunction, IL-33, Endocan