Inflammatory Mediators Associated With Infection by Respiratory Syncytial Virus

Study Overview
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Brief Summary
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Infection with human respiratory syncytial (RS) virus is the most common cause of hospital stay due to pediatric lower respiratory tract infection. An exaggerated immune response contributes to the pathogenesis and small children may have over reactive airways for a long time after an infection. New research has shown that polymorphonuclear leukocytes (PMNs) are stimulated by the virus. Besides fighting the infection they also cause collateral damage to the host. Among other mechanisms PMNs stimulates mucus formation that affects breathing. They also secrete enzymes, toxic proteins and free radicals that may cause harm to lung tissue and airways. The current project strives towards identifying and quantifying inflammatory mediators in sputum, urine and blood of children with severe RS-virus infection. The ultimate aim of the project is to, in detail, describe proteins contributing to the pathogenesis of the disease.

Official Title
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Inflammatory Mediators Associated With Infection by Respiratory Syncytial Virus

Conditions
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Respiratory Tract Infections, Respiratory Syncytial Virus Infections

Intervention / Treatment
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* Other: RS-virus infection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Admission to pediatric intensive care unit Clinical need for invasive ventilation Clinical need for intravascular catheterization Clinical need for urine bladder catheterization Patients with verified or suspected RS-virus-infection or no respiratory tract infection (control group) Exclusion Criteria: • Chronic inflammatory lung disease
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| RS-virus infection with mechanical ventilation<br>Patients admitted to the pediatric ICU with verified or suspected RS-virus infection that are mechanically ventilated. | Other: RS-virus infection<br>* The intervention consists of lower respiratory tract infection due to RS-virus<br>|
| Non-RS-virus infection with mechanical ventilation<br>Patients admitted to the pediatric ICU due to a cause other than a verified or suspected respiratory tract infection. | Other: RS-virus infection<br>* The intervention consists of lower respiratory tract infection due to RS-virus<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Levels of inflammatory mediators in sputum | Simultaneous detection and quantification of hundreds of potential mediators using mass-spectrometry | Up to three weeks | 
| Levels of inflammatory mediators in blood | Simultaneous detection and quantification of hundreds of potential mediators using mass-spectrometry | Up to three weeks | 
| Levels of inflammatory mediators in urine | Simultaneous detection and quantification of hundreds of potential mediators using mass-spectrometry | Up to three weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Disease severity as measured by sequential organ failure assessment score (SOFA-score) |  | Up to 30-days | 
| Lung function as measured in respirator |  | Up to 30-days | 
| Lung function as measured by spirometry |  | Within 1 year | 
| Lung function as measured by spirometry |  | Within 10 years |