Time Lag for Oocyte Denudation: A Better Choice for ICSI Outcome?!

Study Overview
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Brief Summary
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This study aiming to investigate if difference in the timing of cumulus removal post retrieval would have any critical impact on fertilization and embryonic development.

Detailed Description
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Patients under 37 year old who were treated with controlled ovarian stimulation for ICSI were randomly divided into 2 groups; Group A: Oocytes were denudated immediately after oocyte retrieval , Group B: Oocytes were denudated 2 hours after retrieval and both groups were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups.

Official Title
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Time Lag for Oocyte Denudation:Is it a Better Choice for ICSI Outcome?!

Conditions
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Infertility, Female

Intervention / Treatment
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* Procedure: denudation time lag


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Women 24-37 years old With good ovarian reserve Induction using Long agonist protocol unexplained infertility Exclusion Criteria: severe male factor Testicular samples for ICSI poor ovarian reserve no of oocytes collected less than 4

Ages Eligible for Study
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Minimum Age: 24 Years
Maximum Age: 37 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group A (immediate denudation)<br>Oocytes were denudated immediately after oocyte retrieval and were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups. | Procedure: denudation time lag<br>* The time between ovum pickup and denudation of oocyte<br>|
| Group B ( 2hs denudation after ovum pickup)<br>Oocytes were denudated 2 hours after retrieval and were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups. | Procedure: denudation time lag<br>* The time between ovum pickup and denudation of oocyte<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Day 5, good quality blastocyst | Assessment were done according to gardener classification | 120 hour post ovum pickup | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| clinical pregnancy | Assessment was done by observation of a gestational sac at 6 wks | 6 weeks from the last normal menstrual period | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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denudation timing ICSI timing oocyte aging