Relationship Between the VEGF Gene Polymorphism and Cardiometabolic Risk Factors

Study Overview
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Brief Summary
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The aim of the study is to determine the relationship between VEGF gene expression, VEGF gene polymorphism and serum leptin concentration in the Polish population in people with excessive body weight. In addition, the aim of the study is to look for relationships between the VEGF gene polymorphism and anthropometric and biochemical factors of cardiovascular risk and endothelial dysfunction such as body weight, waist circumference, serum total cholesterol, LDL, HDL, triglycerides, glucose and the occurrence of cardiovascular diseases in the family of a patient with excessive body weight in the Polish population.

Detailed Description
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400 people (250 - study group; 150 control group) were subjected to subjective and objective action. Information on the occurrence of diseases and cardiovascular risk in the participant and his family was collected from research studies. Anthropometric parameters were measured (body weight, height, BMI, waist circumference, neck circumference) as well as blood pressure and pulse measurement. In addition, fasting venous blood was collected and secured. In venous blood currently marked with the following concentration: glucose, required cholesterol, LDL cholesterol, HDL cholesterol and triglycerides. In addition, a method of salting out and protecting DNA was developed from blood. Furthermore the polymorphisms of VEGF genes (in positions: -2578 and -634) using the HMR (High Resolution Melt) method was determined. Also VEGF and leptin by ELISA was determined. After obtaining the results of comparative analysis of the correlation between the occurrence of VEGF gene polymorphisms, serum levels of leptin and VEGF, and anthropometric and biochemical parameters of cardiovascular risk.

Official Title
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Relationship Between the VEGF Gene Polymorphism and Cardiometabolic Risk Factors in People With Excessive Body Weight in the Polish Population

Conditions
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Polymorphism, Restriction Fragment Length

Intervention / Treatment
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* Genetic: gene expression


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: informed consent in writing stable body mass (+/- 1 kg) excessive body mass (BMI ≥ 25 kg/m2- study group) proper body mass (BMI < 25 kg/m2- control group) Exclusion Criteria: age <18 years secondary obesity pregnancy, lactation other conditions that in the opinion of researchers may pose any risk to the patient during the study.
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Study group<br>women and men with excess body mass | Genetic: gene expression<br>* determination of VEGF gene expression, VEGF gene polymorphism and serum leptin concentration<br>|
| Control group<br>women and men with proper body mass | Genetic: gene expression<br>* determination of VEGF gene expression, VEGF gene polymorphism and serum leptin concentration<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| VEGF gene expression | VEGF gene expression | through study completion, an average of 1 year | 
| VEGF gene polymorphism | VEGF gene polymorphism | through study completion, an average of 1 year | 
| serum leptin concentration | serum leptin concentration | through study completion, an average of 1 year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Body mass (BM) | Body mass | through study completion, an average of 1 year | 
| Waist circumference (WC) | Waist circumference | through study completion, an average of 1 year | 
| total cholesterol (TCH) | Blood concentration of total cholesterol | through study completion, an average of 1 year | 
| low density lipoprotein (LDL) | Blood concentration of low density lipoprotein | through study completion, an average of 1 year | 
| high density lipoprotein (HDL) | Blood concentration of high density lipoprotein | through study completion, an average of 1 year | 
| triglycerides | Blood concentration of triglycerides | through study completion, an average of 1 year | 
| glucose | Blood concentration of glucose | through study completion, an average of 1 year | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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VEGF gene expression, VEGF gene polymorphism, leptin