Impact of COPD on Muscle Regeneration

Study Overview
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Brief Summary
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Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD) and can affect both respiratory and peripheral muscles. Impaired muscle regeneration is thought to be implicated in the process of muscle atrophy. In this study the investigators will evaluate muscle regenerative potential of the Vastus Lateralis (VL) and diaphragm of patients with COPD, compared to patients with normal lung function.

Detailed Description
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Preliminary results suggest that the activation and differentiation processes of satellite cells isolated from the VL of patients with COPD could be altered. The investigators propose to compare the regenerative potential of the VL and the diaphragm in 15 patients with COPD and 15 individuals with normal lung function undergoing a thoracic surgery. From muscle biopsies, satellite cells will be isolated and cultured to assess their proliferation and differentiation rates, and the expression level of myogenic effectors (MyoD, Myf5, myogenin, and MRF4). Alterations in these measurements will confirm the hypotheses that the regenerative potential is lower in both muscle groups in patients with COPD compared to controls and that in COPD, the regenerative potential is lower in VL compared to the diaphragm.

Official Title
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Impact of Chronic Obstructive Pulmonary Disease on Peripheral and Respiratory Muscle Regeneration

Conditions
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COPD

Intervention / Treatment
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* Procedure: Muscle biopsies


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient with a scheduled thoracic surgery ≥ 40 years Smoking cessation ≥ 1 month Smoking history ≥ 10 pack-year Exclusion Criteria: Subjects who received either chemiotherapy or radiotherapy Hypoxemia Body weight loss ≥ 10% Use of systemic corticosteroid in the last month

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| COPD patients<br>Patients with mild to moderate COPD having a scheduled lung surgery. Vastus lateralis and diaphragm muscle biopsies performed during surgery | Procedure: Muscle biopsies<br>* Muscle biopsies will allow acces to samples for observational studies. Vastus latetalis: needle biopsy (Bergstrom technique). Diaphragm: open biopsy performed by thoracic surgeon during lung surgery.<br>|
| Patients with normal lung function<br>Patients with normal lung function having a scheduled lung surgery. Vastus lateralis and diaphragm muscle biopsies performed during surgery. | Procedure: Muscle biopsies<br>* Muscle biopsies will allow acces to samples for observational studies. Vastus latetalis: needle biopsy (Bergstrom technique). Diaphragm: open biopsy performed by thoracic surgeon during lung surgery.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle satellite cell proliferation index | Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the proliferation capacity | Sequential analyses performed over a period of 2 weeks after tissue collection. | 
| Muscle satellite cell differentiation index | Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the differentiation capacity | Sequential analyses performed over a period of 2 months after tissue collection. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Muscle regeneration