Effect of Lumacaftor-ivacaftor on Glucose Handling and Tolerance in Cystic Fibrosis Phe508del

Study Overview
=================
Brief Summary
-----------------
The purpose of this research study is to find out if the combined therapy lumacaftor-ivacaftor effects how people with cystic fibrosis respond to an oral glucose tolerance test, a test for diabetes.

Detailed Description
-----------------
This is a single center, open label study with crossover in patients with Cystic Fibrosis (CF). Patients will have 2-3 visits at the Diabetes Center at Massachusetts General Hospital (MGH). The participants will have been previously screened clinically to make sure they are candidates for starting the combination drug, lumacaftor-ivacaftor. These patients will be contacted prior to their first visit to discuss enrollment in the study. At the first study visit (Visit 1), the participant will come to the Diabetes Center after an overnight fast of at least 8 hours. The following will occur at this study visit: informed consent; brief medical history; weight and height; vital signs and blood pressure; blood draw for DNA extraction, and an extra research tube for storage; administration of 75g Glucola load as per a standard oral glucose tolerance test (OGTT) protocol; and blood work for glucose and insulin at 30, 60, 90 and 120 minutes after the glucose load. This will be scheduled at a time that is convenient to the patient, with an attempt to coordinate with the patient's visit to the CF clinic prior to starting lumacaftor-ivacaftor combination drug. At the 2nd study visit (Visit 2), which will take place 3 months after starting the combination drug, the participant will again come to the Diabetes Center after an overnight fast of at least 8 hours. The participant will undergo a second OGTT as in the first visit. The 3rd study visit (Visit 3) will be 6 months after initiation of the drug, and will have a repeat administration of an OGTT. If a participant starts the combination drug before enrolling in the study, he/she can still participate in the study as long as he or she has had a clinical OGTT performed within 6 months of starting the combination drug. In these cases, the informed consent, brief medical history, weight and height and vital signs and blood pressure, as well as blood draw for DNA will occur on the Visit 2, which will be the first study visit for these participants. If a clinical OGTT had been performed prior to but within 6 months of starting the combination therapy, this OGTT can be used in analysis of the data, although will not have the full amount of data as the study OGTT.

Official Title
-----------------
A Study of the Effect of Combination Lumacaftor and Ivacaftor on Glucose Tolerance in Persons With Cystic Fibrosis Who Are Homozygous for the Phe508del Cystic Fibrosis Transmembrane Conductance Regulator Mutation.

Conditions
-----------------
Diabetes, Cystic Fibrosis

Intervention / Treatment
-----------------
* Drug: Lumacaftor-ivacaftor
* Other: OGTT


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Age 18 years old or greater Patients diagnosed with CF, genotype homozygous PheDel508 Subject is planning on starting lumacaftor-ivacaftor for clinical reasons, with no contraindication for starting the drug* OR subject is taking combination drug and had an OGTT done at a partners facility in the 6 months prior to initiating the drug. Contraindications for taking drug include abnormal liver enzyme tests, renal dysfunction, pregnancy or nursing mothers Exclusion Criteria: Currently taking any medications for diabetes (including oral or injectable antihyperglycemic agents and/or insulin). Had an admission for CF exacerbation less than 2 weeks prior to staring the medication. This will be defined as requiring new IV or PO antibiotics different than those used in maintenance therapy. Is currently taking oral glucocorticoids or has been on oral or IV glucocorticoids in the past 2 weeks.

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
No

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Screening
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Lumacaftor Ivacaftor<br>Subjects will get an OGTT before and after starting the combination therapy lumacaftor-ivacaftor. | Drug: Lumacaftor-ivacaftor<br>* Subjects who are planning on starting the combination therapy (lumacaftor-ivacaftor) will participate in OGTTs before and after starting the medication.<br>* Other names: Orkambi;Other: OGTT<br>* A subject is given an oral glucose load and insulin and glucose measurements are taken at specified time periods.<br>* Other names: Oral Glucose tolerance test;|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in Fasting Glucose | This will be compared from baseline to 3 months after starting the medication | 3 months | 
| Change in Fasting Glucose | This will compare baseline to 6 months after starting the medication | 6 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Genetic risk score | We will examine how the OGTT data is dependent on genotype at variants associated with type 2 diabetes using a genetic risk score | 6 months | 
| Pulmonary function test (PFT) FEV1 measurements | We will compare how PFT measurement of FEV1 are related to changes in OGTT | 6 months | 
| Change in Fasting Insulin | This will be compared from baseline to 3 months after starting the medication | 3 months | 
| Change in Fasting Insulin | This will be compared from baseline to 6 months after starting the medication | 6 months | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
Genetics