Evaluating MMPs in Burns

Study Overview
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Brief Summary
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The objective of this study is to determine whether our chemical assay can be used to reliably predict graft take or failure in patients undergoing autologous skin grafting for treatment of acute burn injury.

Detailed Description
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This is an observational pilot study of patients treated at UF Health Shands Burn Center adult service. This is a study to determine the predictive value of quantifying concentrations of specific MMPs. Coupling this with a specialized collection sample collection system and a calibrated fluorimetry allows for the rapid assessment of MMP concentrations. This assay has been validated as a predictor of failed wound healing in a published clinical study involving chronic open wounds.

Official Title
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Evaluating a Point-of-Care, Quantitative Matrix Metalloproteinase Assay as a Predictor of Successful Graft Take in Patients Undergoing Cutaneous Auto Grafting for Acute Burn Injury- A Pilot Study

Conditions
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Burns, Thermal Burn, Thermal Injury

Intervention / Treatment
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* Procedure: Graft


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 years of age or older Admitted with acute burns injuries (flame, contact or scald) requiring at least one surgical excision and grafting operation will be considered for inclusion Burn injury less than one week old Exclusion Criteria: Electrical, chemical or cold-induced burn injury Total body surface area burn >50 % Pre injury diagnosis of chronic renal insufficiency, liver failure, refusal to accept blood transfusion or withdrawal of care within three days of admission will also be exclusion criteria Patients not expected to survive Patients presenting with re-injury to previously burned and treated areas. Patients who present after receiving burn excision surgery out an outside facility for the current acute injury

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 98 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients admitted to the burn center<br>Patients admitted to the Burn Service with acute burn injuries requiring at least one surgical excision and grafting operation. | Procedure: Graft<br>* Autogenous skin grafting is the only definitive treatment for the full thickness burn injuries, and thus represents the centerpiece of modern burn care.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Predictability of graft take | Determine whether chemical assay can be used to reliably predict graft take or failure in patients undergoing autologous skin grafting for treatment of acute burn injury. | 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Wound healing | Assess the predictive value of serial MMP assays in identifying wounds transitioning from likely-to-fail to likely-to-succeed | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Matrix Metalloproteinases