Autologous Tolerogenic Dendritic Cells for Rheumatoid Arthritis (AutoDECRA)

Study Overview
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Brief Summary
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This is a study which will look at safety, feasibility and acceptability of a new therapy called tolerogenic dendritic cells (TolDC), derived from the patient's own white blood cells, which will be injected into the knee joints of rheumatoid arthritis patients, using a procedure called arthroscopy (a camera examination of a joint). The investigators are also looking to see if the drug has any effect on the disease activity (if it can help in RA) and whether the drug can affect the immune system. The investigators aim to treat 12 patients in total, 9 with TolDC and 3 with a control treatment. Three doses of TolDC will be tested, 3 patients per dose. Subjects will have RA and at least one swollen knee joint. They will undergo a knee ultrasound scan, fill in a series of questionnaires, have their knee aspirated (fluid taken out) and finally undergo a procedure called leukapheresis (removal of white blood cells) from which the treatment will be manufactured. Subsequently they will undergo 3 arthroscopies (camera examination of the knee joint) over a period of about 12 weeks. On the first arthroscopy they will have the TolDC injected into their knee joint. They will then spend the night at the Clinical Research facility for observation. Over the next 5 days they will be telephoned daily by the study doctor to check how they are, and will be reassessed if needed. About 2 weeks later they will have their second arthroscopy to look for effects of treatment, and the third will take place at 13 weeks (end of study) or sooner if the knee appears to get worse.

Official Title
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Phase 1 Study of Autologous Tolerogenic Dendritic Cells for Rheumatoid Arthritis

Conditions
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Rheumatoid Arthritis

Intervention / Treatment
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* Drug: TolDC
* Drug: Arthroscopy & saline irrigation alone


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Able and willing to give informed consent and to comply with the study protocol At least 6 month's disease duration ACR Functional Class I-III Age 18 years or over Active disease, including an inflamed (native) knee joint Failure (or intolerance of) at least one disease modifying anti-rheumatic drug (DMARD), including current therapy Morning stiffness in the target joint ≥ 30 minutes Willing and able to undergo arthroscopic procedures under local anaesthetic Stable dose of non-steroidal anti-inflammatory drug (NSAID) or corticosteroid (prednisolone≤10mg) for ≥4 weeks No intramuscular glucocorticoid administration for ≥ 6 weeks Stable dose of disease-modifying anti-rheumatic drug (DMARD) for ≥8 weeks Exclusion Criteria: Use of other investigational medicinal products within 30 days prior to study entry (defined as date of recruitment into study) Patients who have received rituximab therapy and whose B-cell count remains below the normal range. Patients who have received any other cell depleting therapies and whose cell counts have not returned to the normal range, at the discretion of the principal investigator. Serious or unstable co-morbidity deemed unsuitable by PI, eg. COPD, cardiac failure History of malignancy (except treated basal cell carcinoma of skin) Known active infection at screening visit or at baseline (except fungal nail infection) Infection requiring hospitalization or IV antibiotics within 6 weeks of baseline Immunization with live vaccine within 6 weeks of baseline History of recurrent or chronic infection History of hepatitis B or C, syphilis, HIV or HTLV-1/2 infections Injection of target joint with glucocorticoids within 6 weeks of baseline Hb<10g/dL; neutrophils< 2.00 x109/L; platelets <150x109/L; ALT/ALP>2x upper limit of normal; elevated serum creatinine at screening visit. Major surgery within 8 weeks of baseline or planned within 3 months from baseline Pregnancy, or women planning to become pregnant within the study period, or women who are breast feeding Females or males of child bearing potential unwilling to use adequate contraception for duration of study Patients taking anticoagulants Known hypersensitivity to local or systemic corticosteroid therapy or local anaesthetic. Poor venous access or medical condition precluding leukapheresis

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: TolDC<br>Experimental arm - TolDC administered arthroscopically | Drug: TolDC<br>* Tolerogenic dendritic cells (TolDC), derived ex vivo from autologous monocytes, administered arthroscopically into an inflamed knee following saline irrigation.<br>|
| Placebo Comparator: Control<br>Arthroscopy & saline irrigation alone | Drug: Arthroscopy & saline irrigation alone<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The proportion of patients experiencing AEs and SAEs following the intra-articular administration of TolDC. |  | within 3 months of injection | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| • The proportion of RA patients who enter the study, from whom GMP-grade TolDC of sufficient quality can be prepared (the success rate of the TolDC preparation procedure). |  | Within one week of donation of blood for extraction of TolDC | 
| • The proportion of patients who grade the trial and its related procedures as acceptable. | Trial participants will assess acceptability of study specific procedures via an 'acceptability questionnaire' administered at the last study visit | At final study visit, 3 months after study intervention. | 
| Systemic RA disease activity | Disease Activity Score using 28 joints (DAS28) | At baseline and at 7, 14 and 91 days post intervention | 
| Knee function | Function will be assessed using the Health Assessment Questionnaire (HAQ), a standard instrument used in RA | At baseline and at 7, 14 and 91 days post-intervention | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Arthritis, RA, Auto-immune disorders