Impact of Chronic Rhinosinusitis on the Index of Ciliary Beat Efficiency Using Fluorescent Nanosticks: (R-IMPAC)

Study Overview
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Brief Summary
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Impact of chronic rhinosinusitis on the index of ciliary beat efficiency using fluorescent nanosticks

Detailed Description
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To date, the assessment of ciliary beat is only possible ex vivo on epithelial cells obtained from upper airway brushing. A previous prospective study (I-IsBac) showed a change in ciliary beat (in terms of coordination and frequency) in bacterial rhinosinusitis. The study of ex-vivo ciliary movement appears to be an interesting tool to understand the pathophysiology of CSRs and to guide and evaluate treatment. A new tool to evaluate the effectiveness of the ex-vivo lash beat has been developed. This tool measures shear stress by tracking balls along the ciliary margin. This measurement of ex-vivo shear stress by bead tracking is a validated technique. However, microbead tracking is limited by its low spatial and temporal resolution, long measurement time and heavy post-processing of acquisition data, making this method difficult to use in clinical routine. Monitoring ex-vivo fluorescent nano-batons could represent a simpler alternative for the clinician. This measurement is now made possible by Phosphate Lanthanum Lanthanum Nano-batons (LaPO4) whose luminescence is directly proportional to the shear. The objective of this research project is to validate this new tool in patients with CSR by comparing it to a group of control subjects free of nasal inflammation.

Official Title
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Impact of Chronic Rhinosinusitis on the Index of Ciliary Beat Efficiency Using Fluorescent Nanosticks

Conditions
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Chronic Rhinosinusitis (Diagnosis)

Intervention / Treatment
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* Procedure: nasal brushing and bacteriological sample


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient ≥ 18 years old Having chronic rhinosinusitis (RCS) requiring surgical treatment. Or With an indication for endonasal surgery for nasal obstruction (turbinoplasty and septoplasty) or for an endonasal surgical approach Acceptance to participate in the protocol with signed informed consent Affiliated or beneficiary of a social security scheme Exclusion Criteria: Refusal to participate in the study No one who does not speak or understand French Pregnant or breastfeeding woman Persons who are incapable of reaching the age of majority, under guardianship, guardianship or protection of justice. Simulated participation in another intervention research

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Patient with Chronic Rhinosinusitis (CRS)<br>Patients with endonasal surgery scheduled under general anesthesia for an indication of Chronic Rhinosinusitis (CRS) | Procedure: nasal brushing and bacteriological sample<br>* A nasal brushing as well as a bacteriological sample using a swab under general anesthesia at the beginning of the endonasal surgery procedure<br>|
| Other: Patient without Chronic Rhinosinusitis (CRS)<br>Patients with endonasal surgery scheduled under general anesthesia for an indication of endonasal surgery for nasal obstruction or patients requiring an endonasal surgical approach such as pituitary adenomas for example. | Procedure: nasal brushing and bacteriological sample<br>* A nasal brushing as well as a bacteriological sample using a swab under general anesthesia at the beginning of the endonasal surgery procedure<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The shear stresss by tracking nano-batons (mPa) | The value of the shear stress by tracking nano-batons in mPa | Day of the surgical procedure under General Anesthesia | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Mucosal inflammation score (0 to 10) | Mucosal inflammation score and presence of polyps during endoscopy | Inclusion Visit | 
| Presence of pus at endoscopy | Presence of pus at endoscopy | Inclusion Visit | 
| Frequency of ciliary beat | Frequency of the ciliary beat | Day of the surgical procedure under General Anesthesia | 
| Shear stress by Tracking microbeads (mPa) | Value of shear stress by tracking microbeads in mPa | Day of the surgical procedure under General Anesthesia | 
| Presence of polyps at endoscopy | Presence of polyps at endoscopy | Inclusion Visit | 
| coordination of cillary beat | Cillary beat coordination evaluated by microscopy | Day of the surgical procedure under General Anesthesia |