The Lung Microbiome in Sarcoidosis

Study Overview
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Brief Summary
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The objective of this study is to determine the presence of bacterial species, and the immunologic and molecular mechanisms of granuloma formation that might be involved in the pathogenesis of sarcoidosis. The investigators will collect peripheral blood and bronchoalveolar lavage fluid samples to isolate bacterial DNA and blood monocytes and lymphocytes. Specimens will be obtained from patients with sarcoidosis, with non-sarcoid lung disease, and healthy volunteers to directly compare the bacterial species present and the characteristics of cells thought to be involved in granuloma formation.

Official Title
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The Lung Microbiome in Sarcoidosis

Conditions
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Sarcoidosis

Intervention / Treatment
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* Other: Blood draw
* Procedure: Bronchoscopy
* Behavioral: Questionnaires


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Clinical diagnosis of sarcoidosis (30 subjects) or Clinical diagnosis of non-sarcoid interstitial lung disease (10 subjects) or Age-matched controls (30 subjects) Ability to provide informed consent Exclusion Criteria: Any disease condition (such as anemia, unstable cardiac disease, etc.) that in the opinion of the subject's primary physician would put the patient at risk of harm from having 60 cc of blood drawn or for undergoing bronchoscopy for this study. Patients who are undergoing bronchoscopy for presumed infection. Pregnant women, women who may be pregnant, and women who are breastfeeding. Patients who have undergone lung transplantation. Persons who are unable or unwilling to undergo bronchoscopy, including those unable to be accompanied by a designated driver to the procedure. Persons who have taken antibiotics in the 90 days prior to consent. Known HIV, HCV, alcohol, or other substance abuse history.

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Blood only<br>20 sarcoidosis subjects and 20 healthy controls will complete lifestyle questionnaires and have blood drawn for microbiome analyses. | Other: Blood draw<br> <br> Behavioral: Questionnaires<br> <br> |
| Blood and BAL<br>10 sarcoidosis subjects, 10 healthy controls, and 10 subjects with non-sarcoid interstitial lung disease will complete lifestyle questionnaires and undergo blood sampling for microbiome analyses as well as bronchoscopy for bronchoalveolar lavage microbiome analyses. | Other: Blood draw<br> <br> Procedure: Bronchoscopy<br> <br> Behavioral: Questionnaires<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Microbiome analysis | To assess the microbiome profile in lung lavage fluid and peripheral blood of subjects | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measures of immunologic phenotype | To determine whether the immune cell profile of sarcoidosis patients differs from healthy controls and other interstitial lung disease patients in blood and BAL fluid | Baseline |