The Effect of Menstrual Cycle Phase and a Protein-polyphenol Drink on Muscle Protein Synthesis

Study Overview
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Brief Summary
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Amino acid (building blocks of protein) and polyphenol supplements are commonly consumed post resistance exercise in order to aid muscle recovery. Both supplements have been shown to be beneficial in isolation, however, there is little known about the effect on recovery of combining the two. Muscle recovery is related to the ability to build new muscle protein from amino acids (muscle protein synthesis). This response is thought to be impacted in females by hormones that fluctuate across different phases of the menstrual cycle. However, the effect of menstrual cycle phase on muscle protein synthesis during recovery is not known.

Official Title
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The Effect of Menstrual Cycle Phase on Muscle Protein Synthesis in Response to Exercise and a Protein-polyphenol Drink in Young Females

Conditions
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Muscle Protein Synthesis

Intervention / Treatment
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* Dietary Supplement: Bolus ingestion of protein
* Dietary Supplement: a blend of protein and polyphenol


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI between 18 and 30 Recreationally active Regular menstrual cycle Exclusion Criteria: Smoking Metabolic disease Any motor disorder Use of over-the-counter pharmaceuticals, during the study. Pomegranate, milk or lidocaine allergy Taken contraceptives in the past 3 months Irregular menstrual cycle (less than 9 cycles per year and a length not between 21-35 days) Post-menopausal women Pregnancy and lactation

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Protein and early<br> | Dietary Supplement: Bolus ingestion of protein<br>* Different phases of the menstrual cycle<br>|
| Experimental: Protein and late<br> | Dietary Supplement: Bolus ingestion of protein<br>* Different phases of the menstrual cycle<br>|
| Experimental: Protein-polyphenol and early<br> | Dietary Supplement: a blend of protein and polyphenol<br>* a blend of protein and polyphenol<br>|
| Experimental: Protein-polyphenol and late<br> | Dietary Supplement: a blend of protein and polyphenol<br>* a blend of protein and polyphenol<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein synthesis | The rate of synthesis of new muscle protein | 7.5 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma amino acid kinetics | The appearance of amino acids in the circulation. | 7.5 hours | 
| Serum insulin concentrations | Basal and postprandial concentrations of serum insulin | 7.5 hours | 
| Blood flow | Basal and postprandial blood flow using ultrasound | 7.5 hours | 
| Sex hormone concentrations | Serum oestrogen and progesterone | 5 minutes |