Diagnostic Study of Gene Alterations in Patients With Acute Myeloid Leukemia

Study Overview
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Brief Summary
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RATIONALE: Diagnostic procedures, such as genetic testing, may improve the ability to detect acute myeloid leukemia and determine the extent of disease. PURPOSE: Diagnostic study to try to detect changes in the genes of patients who have acute myeloid leukemia.

Detailed Description
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OBJECTIVES: Determine the frequency of partial tandem duplication of ALL1 (MLL) in patients with acute myeloid leukemia (AML). Determine the predictive value of partial tandem duplication of ALL1 to identify a subset of AML patients who have a short duration of complete remission. OUTLINE: All samples are obtained from specimens collected on CALGB-9665. No additional blood or bone marrow samples are collected. Samples are examined by Southern blot analysis for gene rearrangement at 11q23. Samples showing evidence of ALL1 gene rearrangement are further analyzed by reverse transcription PCR amplification and/or cytogenetic analysis to detect partial tandem duplication of ALL1.

Official Title
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Assessment of the Partial Tandem Duplication of ALL1 (MLL) in Patients With Acute Myeloid Leukemia- A CALGB Leukemia Tissue Bank Project

Conditions
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Leukemia

Intervention / Treatment
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* Genetic: reverse transcriptase-polymerase chain reaction


Participation Criteria
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Eligibility Criteria
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DISEASE CHARACTERISTICS: Prior registration on protocol CALGB-9665 Diagnosed with acute myeloid leukemia (AML) Treated on a CALGB AML treatment protocol Diagnostic bone marrow or blood specimen available PATIENT CHARACTERISTICS: Age: 15 and over

Ages Eligible for Study
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Minimum Age: 15 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| blood or bone marrow samples<br>All samples are obtained from specimens collected on CALGB-9665. No additional blood or bone marrow samples are collected CALGB-9769. Samples are examined by Southern blot analysis for gene rearrangement at 11q23. Samples showing evidence of ALL1 gene rearrangement are further analyzed by reverse transcription PCR amplification and/or cytogenetic analysis to detect partial tandem duplication of ALL1. | Genetic: reverse transcriptase-polymerase chain reaction<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| frequency of partial tandem duplication of ALL1 (MLL) |  | Up to 10 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| predictive value of partial tandem duplication of ALL1 |  | Up to 10 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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recurrent adult acute myeloid leukemia, adult acute myeloid leukemia in remission