Impact of Neutrophil Activation in Acute Ischemic Stroke Patients Treated With Endovascular Therapy

Study Overview
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Brief Summary
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Clinical and experimental data suggest that neutrophil activation and extravasation are deleterious in acute ischemic stroke (AIS) involving an increased risk of unfavorable outcome and hemorrhagic transformation (HT). However, clinical trials targeting neutrophil recruitment in AIS patients were negative. Recently, an experimental study has shown that neutrophil activation and transmigration begin immediately after the occlusion. Inhibition of neutrophil recruitment several hours after the start of ischemia appears therefore too late to have a clinical relevance. The objective is to study the time dependent impact of neutrophils in AIS and the predominant mediators in each time point to identify the appropriate therapeutic target and time window.

Official Title
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Neutrophil Activation in Acute Ischemic Stroke Patients Treated With Endovascular Therapy

Conditions
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Acute Stroke

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age >= 18 years old acute ischemic stroke secondary to a large vessel occlusion. Patients hospitalized for endovascular therapy. Exclusion Criteria: Pregnant or breast feeding patient patient under legal protection Patient opposition to participate in this study

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| neutrophil counts | Plasma neutrophil activation bio-markers | before recanalization | 
| neutrophil counts | Plasma neutrophil activation bio-markers | immediately after recanalization | 
| neutrophil counts | Plasma neutrophil activation bio-markers | 24 hours after recanalization | 
| neutrophil protease counts |  | before recanalization | 
| recanalization score | Thrombolysis in Cerebral Infarction classification (TICI) | immediately after endovascular procedure | 
| acute ischemic stroke etiology | as defined by the Trial of Danaparoid (ORG10172) in Acute Stroke Treatment (TOAST classification) | within 24 hours after endovascular procedure | 
| severity of neurologic symptoms | National Institutes of Health Stroke Scale (NIHSS) | baseline | 
| disruption of the blood-brain-barrier | diagnosis on cerebral Computerized Tomography | within 24 hours after endovascular procedure | 
| brain haemorrhage | National Institutes of Health Stroke Scale (NIHSS) | 24 hours after endovascular procedure | 
| Recovery of motor function after stroke | modified Rankin scale | 3 months | 
| mortality |  | 3 months | 
| adhesion molecules counts |  | before recanalization | 
| adhesion molecules counts |  | immediately after recanalization | 
| adhesion molecules counts |  | 24 hours after recanalization | 
| free plasma DNA counts |  | before recanalization | 
| free plasma DNA counts |  | immediately after recanalization | 
| free plasma DNA counts |  | 24 hours after recanalization | 
| neutrophil protease counts |  | immediately after recanalization | 
| neutrophil protease counts |  | 24 hours after recanalization |