Heart and Kidney Ketones Metabolism

Study Overview
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Brief Summary
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Participants will undergo 3 Positron Emission Tomography (PET) scans in 3 different conditions : Fasting without exogenous ketone salt Fasting with exogenous ketone salt supplement Post-Prandial with exogenous ketone salt supplement.

Detailed Description
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This study will help to quantify heart and kidney ketone uptake with or without an exogenous ketone salt with or without food intake in middle-aged adults. Participants will have 3 different PET scan to perform : Fasting without exogenous ketone salt Fasting with exogenous ketone salt supplement Post-Prandial with exogenous ketone salt supplement.

Official Title
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Exogenous Ketone Salt on Heart and Kidney Ketone Metabolism Measured by Positron Emission Tomography

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Fasting with exogenous ketone salt supplement
* Dietary Supplement: Post-Prandial with exogenous ketone salt supplement


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Aged between 45 and 64 Generally Healthy BMI between 18.5 and 27 Exclusion Criteria: All drugs taken on a daily basis Diabetes or pre-diabetes Digestive dysfunction Pregnancy or lactation Allergy to the supplement Participate in an intensive physical exercise training (more than three times a week) Being on a ketogenic diet or consumption of ketogenic supplement. More than 2 alcoholic beverages each day Any clinically significant anomaly in the blood profile Smoking Claustrophobia Being enrolled in another interventional research project or in a PET research project Unable to lie dorsally supine for at least 60 minutes

Ages Eligible for Study
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Minimum Age: 45 Years
Maximum Age: 64 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Each participant will have to do the 3 acute interventions
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: Control<br>Fasting without exogenous ketone salt supplement | |
| Experimental: Fasting with exogenous ketone salt supplement<br>Fasting with exogenous ketone salt supplement | Dietary Supplement: Fasting with exogenous ketone salt supplement<br>* Participants will have to take 2 doses of supplement, one 75 minutes before the scan and one 30 minutes before the scan.<br>* Other names: Post-Prandial with exogenous ketone salt supplement;|
| Experimental: Post-Prandial with exogenous ketone salt supplement<br>Post-Prandial with exogenous ketone salt supplement | Dietary Supplement: Post-Prandial with exogenous ketone salt supplement<br>* Participants will have to take 2 doses of supplement, one 75 minutes before the scan and one 30 minutes before the scan and they will have a liquid meal 20 minutes before the scan.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 11C-Acetoacetate Heart Ketone uptake | Heart ketone uptake measured by PET scan | 60 minutes | 
| 11C-Acetoacetate Kidney ketone uptake | Kidney ketone uptake measured by PET scan | 60 minutes | 
| Cardiac Functions, telediastolic volume | telediastolic volume | 60 minutes | 
| Cardiac Functions, telesystolic volume | telesystolic volume | 60 minutes | 
| Cardiac functions, ejection fraction | Ejection fraction | 60minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma ketone | Plasma ketone concentration | 60 minutes |