The Correlation of Periostin and Renal Pathology in Chronic Kidney Disease Patients

Study Overview
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Brief Summary
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The purpose of this study is to determine the location of periostin and urine periostin level in patients with lupus nephritis and IgA nephropathy.

Detailed Description
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Total of 50 lupus nephritis and IgA nephropathy patients who met the inclusion criteria were included in this study. The information sheet and informed consent will be obtained before collecting the patient's data. All patients were interviewed for collecting demographic data and laboratory testing were reviewed from patient's record. Kidney tissue and urine sample were collected at the biopsy date from patients.Kidney tissue were stained with hematoxylin and eosin (H&E), Masson's trichrome solution and immunohistochemistry for periostin. The location of periostin staining in kidney tissue were assessed by renal pathologist. The total of 50 urine samples from patients and 50 urine samples from healthy controls were measured for periostin level by enzyme-linked immunosorbent assay. The Mann-Whitney rank-sum test was used for comparing urine periostin level between patients and healthy controls and also other variables between patients with urine periostin detection and without urine periostin detection. Spearman correlation coefficients were used test correlations between urine periostin level in patients and other variables. Receiver operating characteristic curves was generated to calculate the area under the curve and find the best cutoff value of urine periostin level for distinguishing patients from healthy controls. P-values < 0.05 were considered statistically significant.

Official Title
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Potential of Periostin as a Tissue and Urinary Biomarker in Lupus Nephritis and IgA Nephropathy Patients

Conditions
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Lupus Nephritis, IgA Nephropathy

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: aged over 18 years at least three glomeruli obtained at biopsy Exclusion Criteria: Patients with urinary tract obstruction Patients with urinary tract infection Patients with kidney transplant Patients with cancer diseases Patients with asthma Patients with advanced heart disease Pregnancy and lactation

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Chronic kidney disease<br>Patients with lupus nephritis and IgA nephropathy | |
| Control<br>Healthy controls | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Urine periostin level in lupus nephritis and IgA nephropathy patients compared with healthy controls |  | 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Correlation of urine periostin level in lupus nephritis and IgA nephropathy patients and other variables |  | 2 years | 
| The best cutoff value of urine periostin level for distinguishing patients from healthy controls |  | 2 years | 
| The variables that involved in urine periostin detection in lupus nephritis and IgA nephropathy patients |  | 2 years | 
| Periostin staining in kidney tissue from lupus nephritis and IgA nephropathy patients |  | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Periostin staining, Urine periostin level, Lupus nephritis, IgA nephropathy