Analysis of SARS-CoV2 Urine Viral Particles and Association With Proximal Tubular Dysfunction

Study Overview
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Brief Summary
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The primary goal is to detect Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV2) urine viral particles in patients in intensive care units, hospitalized for coronavirus Coronavirus Disease-19 (COVID-19) infection, and correlate the presence of the virus in the urine with proximal tubular dysfunction (defined by the association of at least 2 abnormalities: tubular proteinuria, renal phosphate leak, uricosuria, normoglycemic glycosuria, amino aciduria)

Official Title
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Molecular Characterization and Cellular Infection Capacity of Urinary Viral Particles Isolated From SARS-CoV-2 and Association With Proximal Tubular Dysfunction, in Hospitalized Patients With COVID-19

Conditions
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SARS-CoV2 Infection, Proximal Tubule Dysfunction

Intervention / Treatment
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* Other: RT-PCR SARS-COV2 in urine


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Person and / or trustworthy person who has received complete information on the organization of the research and who has not opposed their participation and the use of their data Hospitalized for ≥ 48 hours and ≤ 10 days SARS-CoV-2 positive nasopharyngeal sample Severity criteria: Lung involvement described as compatible with SARS-CoV-2 on radiography or scanner and partial pressure of oxygne (PaO2) / Fraction of inspired oxygen (FiO2) ≤ 300 mmHg and / or respiratory rate (RR) ≥ 30 / min Exclusion Criteria: Known chronic kidney disease, or initial Glomerular Filtration Rate (GFR) <60 ml / min / 1.73m2 or Renal Transplant Known proteinuria Known proximal tubulopathy Treatment with Forxiga

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: RT-PCR SARS-COV2 in urine|proteinuria/renal phosphate leak/uricosuria/glycosuria/ aminoaciduria)|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| SARS-CoV 2 viruria and its correlation with proximal tubule dysfunction | Is the detection of viral particles in urine associated with proximal tubule dysfunction, defined by two or more of the following : proteinuria, phosphate leak, aminoaciduria, hyperuricuria, and/or glycosuria | During the first 10 days of hospitalization | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Capacity of infection of urine viral particles | Study of the capacity for cell infection in vitro by viral particles detected in urine. | During the first 10 days of hospitalization | 
| Sequencing viral particles to identify possible compartmentalization of the virus | Study by sequencing of the compartmentalization of urinary and nasopharyngeal variants of SARS-CoV-2 in patients with positive urine for SARS-CoV-2. | During the first 10 days of hospitalization |