Genetics of Obesity in Chinese Youngs

Study Overview
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Brief Summary
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The purpose of this study is to explore the pathogenesis and genetic susceptibility of obese subjects,providing a convincing argument for further treatment of obesity and metabolic syndrome.

Detailed Description
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Obesity has become a major worldwide challenge to public health, owing to an interaction between the obesogenic environment and a strong genetic contribution.Previous studies found that genetic factors determine 40%-70% of obese phenotype.Under such circumstances, the screening of obesity susceptibility gene is particularly important for society or family to take measures to prevent obesity. Recent extensive genome-wide association studies(GWASs) have identified numerous single nucleotide polymorphisms associated with obesity,but these loci together account for only a small fraction of the known heritable component.Two studies in 2010 Nature,for the first time, put rare copy number variation (CNV)in association with severe early-onset obesity.Their significance lie not only in the discovery of the pathogenic genes of severe early-onset obesity but,more importantly,in providing new strategies for finding out genes that cause complex diseases. Obese patients and healthy lean controls proved by a series of blood biochemical examinations will be enrolled in this study.The present study intends to use the techniques such as enzyme-linked immunosorbent assay, real-time fluorescence quantitative PCR,gene chip,construction of viral vectors,transfection, taking advantage of the established database,by means of serum assays and functional tests, associate copy number variation with obesity phenotype to explain the root cause of obesity.Meanwhile biomarkers, gut flora and genetic risk factors will be evaluated in the study subjects.

Official Title
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Study of Clinical Characteristics and Genetic Susceptibility in Chinese Obese Youngs

Conditions
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Obesity, Metabolic Syndrome

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: body mass index (BMI)≥ 30kg/m2 predominantly east China to minimize population stratification willing and able to provide informed consent Exclusion Criteria: pregnancy/lactation Cushing syndrome Hypothyroidism obesity caused by pituitary and hypothalamic lesions drug related obesity history of major psychiatric illness

Ages Eligible for Study
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Minimum Age: 14 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy lean control group<br>The healthy lean controls consisted of age-, sex- and geography-matched young volunteers, or golden standard lean controls from China Cardiometabolic Disease and Cancer Cohort Study (4C Study). Healthy volunteers: BMI:18.5-22.9kg/m2; Age:14-30 years old; To be proved normal by the examinations of liver and kidney function,blood lipids profile,FBG, PBG, fasting insulin and HbA1c. Controls from 4C Study: 17.0≤BMI≤23.0kg/m2 and waist circumference <85cm for males and <80cm for females; 1.8≤LDL-c≤3.4mmol/l; FBG<6.1, PBG<7.8; HOMA-IR<2.5; BP <140/90mmHg; eGFR>60mL/(min·1.73 m2), ALT<40IU/L, AST<40IU/L; Excluding the history of hyperthyroidism, digestive system diseases, malignant tumors, infectious diseases (hepatitis B) and other systemic wasting diseases; Severe dysfunction of heart, lung and kidney; Not using antihypertensive, hypoglycemic and lipid-lowering drugs； No history of smoking. | |
| obesity group<br>BMI ≥30kg/m2; Age:14-30 years old; obesity group 4000, lean healthy control group 4000 | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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obesity, metabolic syndrome, genetic risk markers, copy number variation