Interleukin-38 Levels in Individuals With Periodontitis

Study Overview
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Brief Summary
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Interleukin (IL)-38 is the newest member of the IL-1 family. It can bind to receptors through various pathways and regulate the formation and function of inflammatory cytokines. Periodontitis is a chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. The aim of this study is to compare the IL-38, IL-1β and IL-10 levels of healthy and periodontitis individuals.

Detailed Description
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Periodontitis is a destructive chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. More than any other cytokine family, the interleukin (IL)-1 family (IL-1α, IL-1β, IL-18, IL-33, IL-36α, IL-36β, IL-36γ, IL-1Ra, IL-36Ra, IL-37, IL-38) includes key signaling molecules that trigger and perpetuate periodontal inflammation. Interleukine -1β ( IL-1β ) levels are associated with periodontal destruction in the pathogenesis of periodontal disease. Interleukine-10 (IL-10) has been reported to decrease periodontal disease progression rate. Gingival crevicular fluid shows the cellular response in the periodontium. The most important host-related ingredients are inflammatory markers, including cytokines, enzymes, and interleukins. Saliva analysis is used in the diagnosis of systemic conditions as well as in the diagnosis of oral pathologies. In recent studies, IL-38 expression has been reported to be abnormal (abnormal) in chronic inflammatory diseases, especially in rheumatoid diseases such as systemic lupus erythematosus, rheumatoid arthritis, psoriasis, and inflammatory bowel disease. Therefore, this relationship of this inflammatory cytokine with chronic inflammatory diseases draws attention to the regulatory effect of IL-38 in these diseases. The aim of this study is to compare the IL-38, IL-1β and IL-10 levels of healthy and periodontitis individuals.

Official Title
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Evaluation of Interleukin-38 Levels in Individuals With Healthy and Periodontal Disease

Conditions
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Periodontitis, Gingivitis, Healthy

Intervention / Treatment
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* Other: Saliva
* Other: gingival crevicular fluid


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: systemically healthy individuals who does not have a disease under treatment, individuals without regular systemic medication Exclusion Criteria: smoking receiving periodontal treatment in the last 6 months pregnancy lactation

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Periodontitis<br>Individuals with Periodontitis | Other: Saliva<br>* individuals' saliva samples will be collected and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.<br>* Other names: sampling;Other: gingival crevicular fluid<br>* GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes.Until biochemical analysis is carried out will be stored in -80ºC.<br>* Other names: sampling;|
| Gingivitis<br>Individuals with Gingival Inflammation | Other: Saliva<br>* individuals' saliva samples will be collected and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.<br>* Other names: sampling;Other: gingival crevicular fluid<br>* GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes.Until biochemical analysis is carried out will be stored in -80ºC.<br>* Other names: sampling;|
| Healthy<br>Individuals with Periodontally Healthy | Other: Saliva<br>* individuals' saliva samples will be collected and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.<br>* Other names: sampling;Other: gingival crevicular fluid<br>* GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes.Until biochemical analysis is carried out will be stored in -80ºC.<br>* Other names: sampling;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| IL-38 levels | saliva and DOS levels | initial examination, baseline | 
| IL-1β levels | saliva and DOS levels | initial examination, baseline | 
| IL-10 levels | saliva and DOS levels | initial examination, baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plaque Index | Silness-Löe | initial examination, baseline | 
| Probing Depth | millimeter | initial examination, baseline | 
| Bleeding on Probe | Ainamo&Bay | initial examination, baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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periodontal disease, IL-38