Pathomechanism of MicroRNA-29 in Shoulder Stiffness

Study Overview
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Brief Summary
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The team integrates experimental analyses of clinical and basic medicine and transgenic mice models. miR-29a acts a potent protective factor against excessive fibrosis in myofibroblasts of subacromial bursa tissue. Gain of miR-29a stabilizes tendon and synovial tissue homeostasis that alleviates tissue stiffness and maintains function.

Detailed Description
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Aims of first: The team will evaluate the associations among the mR-29a, miR-29b, miR-29c expression in subacromial bursa and subacromial fluid, incidence of shoulder stiffness, Constant scores, and VAS. Aims of second: The team will isolate primary myofibroblast from subacromial bursa tissue as in vitro models and investigate the molecular events in the IL-1β modulation of miR-29a expression and the molecular mechanism underlying miR-29a inhibition of fibrotic matrix accumulation and apoptotic reactions in myofibroblast cultures.

Official Title
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Pathomechanism of MicroRNA-29 in Shoulder Stiffness

Conditions
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Stiffness of Shoulder, Not Elsewhere Classified

Intervention / Treatment
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* Biological: miR 29a


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: aged 18 to 80 years receiving surgery for open acromioplasty Exclusion Criteria: shoulder disorders caused by traumatic fracture previous surgery osteoarthritis malignant disorders hepatic disorders renal disorders

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: miR-29a precursor oligonucleotide<br>Effects of IL-1β, miR-29a precursor oligonucleotide treatment on the expressions of miR-29a, miR-29b or miR-29c in cell cultures are quantified | Biological: miR 29a<br>* Effects of IL-1β, miR-29a precursor or antisense oligonucleotide treatment on the expressions of miR-29a, miR-29b or miR-29c in cell cultures are quantified<br>|
| Experimental: miR-29a antisense oligonucleotide<br>Effects of IL-1β, miR-29a antisense oligonucleotide treatment on the expressions of miR-29a, miR-29b or miR-29c in cell cultures are quantified | Biological: miR 29a<br>* Effects of IL-1β, miR-29a precursor or antisense oligonucleotide treatment on the expressions of miR-29a, miR-29b or miR-29c in cell cultures are quantified<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The microRNA expression, mRNA expression as a measure | Differences among incidence, microRNA expression, mRNA expression of shoulder stiffness are analyzed using non-parametric student's t-test. P value of < 0.05 is considered statistically significant. | 48hr | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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MicroRNA-29, shoulder stiffness, mir-29a