Biological Markers Evaluation of Accelerated Tooth Movement

Study Overview
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Brief Summary
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Gingival Crevicular Fluid samples will be collected using periopapers after accelerated tooth movement technique, results will be assessed with biomarkers levels done using ELISA Assays

Detailed Description
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For this prospective study, 22 individuals were selected, with skeletal discrepancies randomly divided into two groups: 1 - accelerated tooth movement technique (ATMT) and 2 - Control. Orthodontic records done in two times, initial and preoperative; Gingival Crevicular Fluid samples will be collected using periopapers in 7 times (1, 2, 7, 14, 28 days and after space closure- end of orthodontic treatment movement activation). Flapless cortical perforations will be made in ATMT Group after dental extraction. Surgical protocol will be flapless to preserve periodontal health. After extraction space closure, results will be assessed by clinical effects with biological markers levels: CCL3/MIP-1 alpha, CCL5/RANTES, CXCL8/IL8, IL-1 alpha, IL-1 beta, IL6, TNF-alpha, TRANCE/RANK L, OCN OPG IL-10 VEGF TGF-ℬ Those biomarkers levels will be performed using ELISA Assays. After collecting data, the results will be statistically analyzed using Shapiro-Wilk and, posteriorly, T-student or Wilcoxon.

Official Title
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Biomarkers Expression in Gingival Crevicular Fluid and Accelerated Tooth Movement

Conditions
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Cytokine Measurements, Orthodontic Space Closure, Tooth Movement

Intervention / Treatment
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* Procedure: micro-osteoperforation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adult subjects ages 18-40 years old, in good general health, with adult complete dentition, regardless of presence of third molars. Indication for orthognathic surgery Tooth extraction indication for orthodontic treatment Do not smoke Periodontal and teeth healthy Do not use bisphosphonates or corticosteroids Exclusion Criteria: Surgically assisted rapid palatal expansion indication Cleft lip and palate Craniofacial syndrome Mental disorder Metabolic diseases

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: Control<br>Control group, with traditional orthodontic mechanics for anterior retraction. Crevicular fluid will be collected during anterior retraction mechanics to identified biomarkers in the inflammatory process | |
| Experimental: Acceleration<br>Group with micro-osteoperforation for accelerated tooth movement during anterior retraction, Crevicular fluid will be collected during anterior retraction mechanics to identified biomarkers in the inflammatory process | Procedure: micro-osteoperforation<br> <br> * Other names: accelerated tooth movement;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Inflammatory Cytokines ad Chemokines Detection Assay | Measure the different levels of listed cytokines and chemokines after starting the anterior retraction | 8 months | 
| Bone remodeling markers in GCF samples | Measure the different levels of resorption biomarkers and bone formation after starting the anterior retraction | 8 monnths |