Expression of microRNA-133a and microRNA-208b in Acute Myocardial Infarction

Study Overview
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Brief Summary
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Cardiac-enriched micro-RNAs (miRNAs), micro RNA 208b and 133a(MiR-208b, MiR-133a)) corresponds to the health and disorders of the cardiovascular system. An intron of the cardiac myosin heavy chain gene MYH7 encodes miR-208b. It is found on chromosome 14 in humans. Identify new diagnostic biomarkers based on miRNAs, researchers examine the expression of miR-133a and 208b at various time points (04 hours, 08 hours, 12 hours, 24 hours, 48 hours) following the development of the infarct and compared it to the traditional myocardial infarction biomarkers cardiac troponine (cTnl) and Creatine kinase-MB (CK-MB).

Detailed Description
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The 98% of human genes are non-coding RNA, which is further divided into long non-coding RNA (lncRNA, > 200 bp) and short non-coding RNA (200 bp, including microRNA (miRNA) and circular RNA). More than 2000 human miRNAs have been discovered, and it seems that they regulate around 60% of human genes.Through a number of pathways, miRNAs control the development, re-modelling, and regeneration of the heart as well as endothelial function, vasculo-genesis, and neoangiogenesis. 30% of the genes in the human genome are regulated by about 1000 miRNAs. Endogenous miRNAs are 19-22 nucleotide single-stranded RNA molecules that frequently target the 3' untranslated regions (3'-UTR) of mRNAs to regulate gene expression at the post-transcriptional level in physiological processes, disease development, and conditions like cancer, autoimmune and inflammatory diseases, neuro-degenerative diseases, and cardiovascular diseases. Cardiac-enriched miRNAs, MiR-208 in health and disorders of the cardiovascular system are investigated. Identify new diagnostic biomarkers based on miRNAs, researchers examine the expression of miR-133a and 208b at various time points (04 hours, 08 hours, 12 hours, 24 hours, 48 hours) following the development of the infarct and compared it to the traditional myocardial infarction biomarkers cTnl and CK-MB.

Official Title
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Expression of microRNA-133a and microRNA-208b in Acute Myocardial Infarction Egyptian Patients

Conditions
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Myocardial Infarction, Acute

Intervention / Treatment
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* Other: No intervention


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients diagnosed with ST-Elevated Myocardial Infarction will be identified at the Coronary Care Unit at Ain-shams University hospital Acute episode of Myocardial Infarction With No history of previous Myocardial Infarction Treatment Exclusion Criteria: Patients not diagnosed with ST-Elevated Myocardial Infarction and healthy at the Coronary Care Unit at Ain-shams 2. University hospital Previously Known patients of Myocardial infacrtion Previous medication

Ages Eligible for Study
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Minimum Age: 35 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Test Group<br>The study group included 10 patients, men and women between age of 35-60 year chest pain lasting for at least 30 minutes, electrocardiogram (ECG) finding that display ST-segment elevation (STEMI) | Other: No intervention<br>* Expression of miR-133a &208b at 4, 8, 12, 24 and 48 hours from the first appearance of myocardial infarction (MI) symptoms<br>* Other names: Expression of miR-133a & 208b;|
| Control Group<br>The study group included 10 patients, men and women between age of 35-60 year having no complaint of health issue, | Other: No intervention<br>* Expression of miR-133a &208b at 4, 8, 12, 24 and 48 hours from the first appearance of myocardial infarction (MI) symptoms<br>* Other names: Expression of miR-133a & 208b;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Expression of miR-133a | Expression during episodes of myocardial infarction | 04 hours from the onset of the infarct | 
| Expression of miR-133a | Expression during episodes of myocardial infarction | 08 hours from the onset of the infarct | 
| Expression of miR-133a | Expression during episodes of myocardial infarction | 12 hours from the onset of the infarct | 
| Expression of miR-133a | Expression during episodes of myocardial infarction | 24 hours from the onset of the infarct | 
| Expression of miR-133a | Expression during episodes of myocardial infarction | 48 hours from the onset of the infarct | 
| Expression of miR-208b | Expression during episodes of myocardial infarction | 04 hours from the onset of the infarct | 
| Expression of miR-208b | Expression during episodes of myocardial infarction | 08 hours from the onset of the infarct | 
| Expression of miR-208b | Expression during episodes of myocardial infarction | 12 hours from the onset of the infarct | 
| Expression of miR-208b | Expression during episodes of myocardial infarction | 24 hours from the onset of the infarct | 
| Expression of miR-208b | Expression during episodes of myocardial infarction | 48 hours from the onset of the infarct |