The Effect of Chewing the Tapioca Pearls in Bubble Tea Drinks

Study Overview
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Brief Summary
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Bubble tea drinks contain tea and tapioca pearls. Chewing tapioca pearls in bubble tea drinks may increase salivary components. Because of its proteins, inorganic components, and enzymes, saliva plays an important role in the body's defense against bacteria and viruses. This study aims to analyze the effect of chewing the tapioca pearls in bubble tea drinks on salivary C-reactive protein (CRP) and calcium (Ca) levels.

Detailed Description
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Sixty saliva samples were collected from 15 subjects. The inclusion criterion was 18-25 years of age. The exclusion criteria were receiving medication, using dentures, a history of dry mouth, smoking and systemic disease. In the first week of the experiment, subjects drank bubble tea as the intervention group. In the second week, the same subjects drank tea without pearls as the control group. Each subject drank the bubble tea for 5 minutes per day over 3 days. Saliva samples were collected on the first day before bubble tea consumption (pretest) and on the third day after tea consumption (posttest). Saliva collection was performed in the morning (09:00 a.m.-12:00 p.m.) for 1 minute. Salivary CRP levels were measured using an ELISA kit, and Ca levels were determined using a test kit.

Official Title
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Quality Improvement of Saliva by Chewing Tapioca Pearls in Bubble Tea Drinks

Conditions
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Saliva

Intervention / Treatment
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* Other: chewing tapioca pearls in bubble tea drinks
* Other: drink tea without chewing tapioca pearls


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: good Oral Hygiene Index-Simplified (OHI-S) score Exclusion Criteria: taking medication a history of dry mouth smoking systemic disease

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 25 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: In the first week of the experiment, subject chewing tapioca pearl inthe bubble tea. In the second week, the same subjects drink tea without chewing tapioca pearl
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: chewing tapioca pearls in the bubble tea drinks<br>In the first week, the subjects drink as much as 100 ml of bubble tea over a span of 5 minutes once a day for 3 days | Other: chewing tapioca pearls in bubble tea drinks<br>* Saliva was collected on the first day before bubble tea consumption (pretest) and on the third day after bubble tea consumption (posttest); collection was conducted in the morning (09:00 a.m.-12.00 p.m.).<br>|
| Placebo Comparator: drink tea without chewing tapioca pearls<br>In the second week, the subjects drink tea without tapioca pearls as much as 100 ml for 5 minutes per day for 3 days. | Other: drink tea without chewing tapioca pearls<br>* Saliva was collected on the first day before drink tea without tapioca pearl (pretest) and on the third day after tea consumption (posttest); collection was conducted in the morning (09:00 a.m.-12.00 p.m.).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Salivary C-reactive protein (CRP) level | Salivary CRP level was measured on the first day before chewing tapioca peals in the bubble tea drinks and drink tea. Salivary CRP level was measured on the third day after drink bubble tea and tea without tapioca pearls | 3 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Calcium (Ca) level | Ca level was measured on the first day before chewing tapioca peals in the bubble tea drinks and drink tea. Ca level was measured on the third day after drink bubble tea and tea without tapioca pearls | 3 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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bubble tea, salivary C-reactive protein, salivary calcium