Plasma Triglyceride Extraction by The Muscle

Study Overview
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Brief Summary
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Increased accumulation of fat into the muscles is associated with what is called insulin-resistant state, which is a pre-diabetic state. The purpose of this research is to find out how fat circulating in the blood following fat consumption is taken up by the muscles in healthy people as well as people that are insulin-resistant. The investigators are specifically interested in how a hormone called insulin is involved in this process. Findings from this research will contribute to our understanding of why insulin-resistant people have increased accumulation of fat in their muscles, and ultimately help to design appropriate interventions to prevent type 2 Diabetes.

Official Title
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Regulation of the Plasma Triglyceride Extraction in Muscle in Insulin Resistance

Conditions
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Obesity, Insulin Resistance

Intervention / Treatment
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* Drug: Insulin
* Drug: NG-monomethyl-L-arginine


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Body mass index (BMI): < 25 kg/m2 or > 30 kg/m2; Insulin sensitivity index (ISI): insulin-sensitive ISI ≥ 7, insulin-resistant ISI ≤ 5. Exclusion Criteria: Medication or supplements known to affect either endothelial function or lipid metabolism (i.e. arginine, protein, fish oil) Acute illness Uncontrolled metabolic disease, including liver or renal disease Atrial fibrillation, history of syncope, limiting or unstable angina, congestive heart failure, known or suspected right-to-left, bi-directional or transient right-to-left cardiac shunts Cardiac pacemaker or other medical device implanted in the body Pulmonary hypertension or other unstable cardiopulmonary conditions ECG documented abnormalities or valvular disease Low hemoglobin or hematocrit (i.e., lower than accepted laboratory values) History of hypertension or elevated blood pressure (systolic, >140 mmHg or a diastolic, >95 mmHg) History of hyperlipidemia or plasma triglyceride concentration >200 mg/dl Diagnosed diabetes, or 2-h plasma glucose >200 mg/dl during an oral glucose tolerance test Current participation in a weight-loss regimen, including extreme dietary practices Use of anabolic steroids or corticosteroids (within 3 months)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Insulin-Sensitive<br>One group consists of insulin sensitive volunteers. | Drug: Insulin<br> <br> Drug: NG-monomethyl-L-arginine<br> <br> |
| Experimental: Insulin-Resistant<br>One group consists of insulin resistant volunteers. | Drug: Insulin<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Triglyceride extraction across the forearm |  | Measured during a 7-hour infusion study | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle microvascular blood volume |  | Selected 15-minute periods during the 7 hour infusion study |