Side of Embryo Biopsy Interfering Implantation Potential

Study Overview
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Brief Summary
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Selection of side of embryo biopsy that will not interfering with implantation power of developed blastocyst embryo during biopsy procedure arranged for PGT.

Detailed Description
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During process of embryo biopsy, it was indicated to avoid contacting cells closer to inner cell mass as it can decrease the quality of embryo and its structural composition. However, recommendation for collection from the trophectoderm (TE) side is indicated. Absence of differentiation during cleavage-stage or morula stage at day 3 and day 4 respectively, will present the needful to focus a specific location for embryo biopsy during pre-blastocyst stages for embryo going to do genetic testing before implantation or embryo transfer. In articulation around the second polar body or the animal pole of embryo, collection of the cell from the vegetal pole in crospondence to avoide collection form the animal pole is to be compare the results of implantation for biopsy done using the animal pole side or the random non organized biosy procedure.

Official Title
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Effect of Embryo Biopsy Side on Post Biopsy Blastocyst Structurs and Implantation

Conditions
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Preimplantation Diagnosis

Intervention / Treatment
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* Procedure: Vegetal Pole blastomer embryo
* Procedure: Animal Pole blastomer embryo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: grade 1 or 2 embryos at eight cell stage or 8-1/ 8-2 embryo quality. Exclusion Criteria: grade 3 embryo at day 3, or 8-3 embryos.
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Allocation: Non-Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Vegetal Pole blastomer embryo<br>for day 3 embryo biopsy / cleavage stage: Blastomere collection from opposite side to the 2nd polar body or from vegetal pole. | Procedure: Vegetal Pole blastomer embryo<br>* for day 3 embryo biopsy / cleavage stage<br>* Other names: Biopsy embryo from sides considered far from ICM location.;Procedure: Animal Pole blastomer embryo<br>* for day 3 embryo biopsy / cleavage stage<br>* Other names: Random biopsy without considering specific location;|
| Experimental: Animal Pole blastomer embryo<br>for day 3 embryo biopsy / cleavage stage: Blastomere collection from and side around the 2nd polar body and not the vegetal pole. | Procedure: Vegetal Pole blastomer embryo<br>* for day 3 embryo biopsy / cleavage stage<br>* Other names: Biopsy embryo from sides considered far from ICM location.;Procedure: Animal Pole blastomer embryo<br>* for day 3 embryo biopsy / cleavage stage<br>* Other names: Random biopsy without considering specific location;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| blastocyst location of ICM at day 5 | describing the location of the inner cell mass (ICM) in location to the zona peluceda (ZP) drilling opening. | 2 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Implantation power | calculating the pregnancy rate for cases after biopsy. | 2 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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embryo biopsy, preimplantation genetic testing, animal pole, vegetal pole, day 3 embryo biopsy, embryo implantation, Blastomeres