Detection of Chlamydia DNA by Polymerase Chain Reaction in Primary Tubal and Ovarian Cancers Tissues : a Pilot Study

Study Overview
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Brief Summary
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PCR for chlamydia DNA is done in paraffin blocks of cases of primary tubal cancer and is compared to cases of high grade serous ovarian cancer and normal tubes .

Detailed Description
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The investigators shall review the early cancer detection unite pathology records for the past 10 years , for cases diagnosed with primary Fallopian tube cancers , cases of high and low grade serous ovarian cancer and cases underwent hysterectomy and salpingectomy for non oncological reasons. Paraffin blocks for suitable selected patients will be examined histologically to confirm the primary diagnosis . Chlamydia DNA in these paraffin blocks of the Fallopian tubes and ovarian tissues will be examined using PCR techniques . The possible correlation between the presence of Chlamydia DNA and the ovarian serous tumours and primary tubal cancer will be analyzed statistically .

Official Title
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Detection of Chlamydia DNA by Polymerase Chain Reaction ( PCR ) in Tumours Tissue of Primary Tubal and Ovarian Cancers : a Pilot Study

Conditions
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Malignant Neoplasm of Fallopian Tube

Intervention / Treatment
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* Other: PCR for chlamydia DNA in primary tubal cancer


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: any patient with primary tubal cancer and high grade serous cancer and normal tubes. Exclusion Criteria: clinical pelvic inflammatory disease salpingectomy for ectopic

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| primary tubal cancer group<br>Paraffin blocks of cases of primary tubal cancer | Other: PCR for chlamydia DNA in primary tubal cancer<br>* PCR for presence of chlamydia DNA in paraffin blocks retrieved for tubal and ovarian tissues in all groups<br>|
| high grade serous ovarian cancer group<br>Paraffin blocks of cases of high grade serous ovarian cancer | Other: PCR for chlamydia DNA in primary tubal cancer<br>* PCR for presence of chlamydia DNA in paraffin blocks retrieved for tubal and ovarian tissues in all groups<br>|
| Low Grade Serous Ovarian Cancer<br>Paraffin blocks of cases of low grade serous ovarian cancer | Other: PCR for chlamydia DNA in primary tubal cancer<br>* PCR for presence of chlamydia DNA in paraffin blocks retrieved for tubal and ovarian tissues in all groups<br>|
| normal tubes group<br>Paraffin blocks of tubes of hysterectomies due to non-oncologic causes | Other: PCR for chlamydia DNA in primary tubal cancer<br>* PCR for presence of chlamydia DNA in paraffin blocks retrieved for tubal and ovarian tissues in all groups<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Presence of chlamydia DNA in tubal and ovarian tissues |  | Up to 24 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Presence of concomitant tubal cancer with high grade serous ovarian cancer |  | Up to 8 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Fallopian tubal cancer, high grade serous , ovarian cancer