Muscle Response to High Intensity Interval Resistance Training (HIIRT)

Study Overview
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Brief Summary
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Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Detailed Description
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Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30 rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Official Title
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Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training

Conditions
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Exercise, Resistance Training

Intervention / Treatment
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* Other: High Intensity Interval Resistance Training
* Other: Traditional Resistance Training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: moderate active experience with resistance training Exclusion Criteria: history of recent myocardial infarction, severe cardiac arrhythmia, unstable angina, poorly controlled hypertension, poorly controlled diabetes mellitus, frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: High Intensity Interval Resistance Training (HIIRT)<br>HIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30 rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. | Other: High Intensity Interval Resistance Training<br>* Subjects trained one leg with HIIRT<br>|
| Active Comparator: Traditional Resistance Training (TRT)<br>TRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75 of rest between sets. | Other: Traditional Resistance Training<br>* Subjects trained the other leg with HIIRT<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| pAMPKThr172 in skeletal muscle tissue measured by immunoblot | measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy | 24 hours | 
| pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot | measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy | 24 hours | 
| pS6Ser235/236 in skeletal muscle tissue measured by immunoblot | measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy | 24 hours | 
| p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot | measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy | 24 hours | 
| ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot | ACC phosphorylation in skeletal muscle tissue taken by biopsy | 24 hours | 
| IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | IGF-1 mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | IGF-1ea mRNA in skeletal muscle tissue taken by biopsy | 24 hoursn | 
| MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | MGF mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | STARS mRNA in skeletal muscle tissue taken by biopsy | 24 hoursn | 
| Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | Myogenin mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | IL-6 mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | PGC1a4 mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | PGC1a mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | Atrogin mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | Beclin mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 
| Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | Myostatin mRNA in skeletal muscle tissue taken by biopsy | 24 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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resistance training, recovery, muscle hypertrophy, muscle signalling