Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

Study Overview
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Brief Summary
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To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients. To test the effect of ischemic postconditioning on ischemia reperfusion injury in healthy volunteers, using Annexin binding after repetitive handgripping.

Detailed Description
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Recently, ischemic postconditioning has been identified as a protective intervention against ischemia-reperfusion injury. In animal studies, the signalling pathway of this (impressive) protective phenomenon is very similar to ischemic preconditioning. It opens a new avenue of post-reperfusion interventions with drugs that have been shown to mimic ischemic preconditioning. Before we can study this phenomenon in our forearm ischemia-reperfusion model, we need additional validating experiments. The purpose of this study proposal is to provide these data.

Official Title
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Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

Conditions
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Cardiovascular Disease, Ischemia-reperfusion Injury, Postconditioning

Intervention / Treatment
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* Procedure: repetitive short ischemia and reperfusion of non dominant fore arm after 10 minute ischemic exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 18-50 years No physical limitation to perform ischemic exercise Informed consent Exclusion Criteria: Diabetes (fasting glucose > 7,0mmol/l, or random glucose >11,0mmol/l) hyperlipidemia (random total cholesterol > 6.5 mmol/l) Hypertension (supine SBP/DBP > 140/90 mmHg at screening) Any cardiovascular disease Drug abuse Concomitant chronic use of medication Administration of radioactivity in research setting during the last 5 years Participation to any drug-investigation during the previous month as checked with VIP check according to CRCN standard procedures.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Intervention/Treatment |
| --- |
|Procedure: repetitive short ischemia and reperfusion of non dominant fore arm after 10 minute ischemic exercise|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Annexin A 5 targeting in the non dominant thenar muscle after (ischemic exercise), as a indicator for ischemia reperfusion injury. |  |  | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Workload during (ischemic) exercise. |  |  | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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cardiovascular disease, ischemia-reperfusion injury, postconditioning, annexin targeting