The Effect of Beetroot Juice on Plasma Trimethylamine N-oxide Concentration

Study Overview
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Brief Summary
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The primary aim of the current study is to determine the effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration. A secondary aims: exploring the effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status, and measurement of changes in the skin nicotinamide-adenine dinucleotide reduced form (NADH) content during transient ischemia and reperfusion.

Detailed Description
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In a random order subjects will consume beetroot juice (140ml), or equimolar nitrates (NaNO3). Antecubital vein blood samples will be taken at the beginning, and every hour for 4 hours following consumption. Plasma trimethylamine N-oxide, total antioxidant status, and nitrite/nitrate concentrations will be determined. Using Flow Mediated Skin Fluorescence skin NADH content determined during controlled ischemia triggered by total occlusion of the brachial artery by the brachial blood pressure cuff and during reperfusion after deflation of the blood pressure cuff 2 and 4 hours following consumption.

Official Title
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Analysis of Electromyography Activation Trunk Muscles and the Effect of Beetroot Juice Intake on the Results in Strength Exercises: Lateral Medicine Ball Throw and Sled Pushing

Conditions
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Hypertension

Intervention / Treatment
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* Dietary Supplement: beetroot juice
* Other: nitrate


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy volunteers Exclusion Criteria:

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: beetroot juice<br>single intake of beetroot juice containing 800mg nitrates | Dietary Supplement: beetroot juice<br>* single intake of beetroot juice containing 800mg nitrates<br>* Other names: 140 ml of Beet It juice;|
| Active Comparator: nitrates<br>single intake of 800mg nitrates (NaNO3) | Other: nitrate<br>* single intake of 800mg nitrates<br>* Other names: NaNO3;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration | Determination of plasma trimethylamine N-oxide concentration following the beetroot juice consumption using ultra performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) method. | 4 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status | Determination of plasma total antioxidant status following the beetroot juice consumption using commercially available kit. | 4 hours |