Muscle Training Induced Angiogenesis in COPD

Study Overview
=================
Brief Summary
-----------------
COPD patients experiment a peripheral muscle dysfunction which impact their exercise tolerance and health-related quality of life. The capillary to fiber interface is reduced and impact the exercise capacity of the patients. While the muscle capillary creation in response to exercise training is blunted in COPD patients, the maturation of the neo-capillaries could also be blunted and contribute to the impaired aniogionenesis in patients. Because the capillary maturation is a sensitive and dynamic process, only different modalities of exercise training and multiple time-points of measures would allow to capture this microvascular adaptation. Aim of the study : Compare the muscle capillary maturation in response to training at 5 and 10 weeks, in sedentary healthy subject trained at the intensity of the ventilatory threshold (60-65% of VO2max), versus :- COPD patients trained at a similar intensity (60-65% of VO2max)- COPD patients trained at a similar absolute intensity (90% of VO2max).

Official Title
-----------------
Impact of Exercise Training on Skeletal Muscle Capillary Maturation During Angiogenesis in COPD Patients

Conditions
-----------------
Peripheral Muscle Dysfunction, COPD

Intervention / Treatment
-----------------
* Procedure: Muscular biopsy
* Other: 5-10 weeks sport training


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: COPD patients : FEV1<80%pred. non exacerbation during the past 4 weeks no oxygen therapy, - Healthy subjects: <150min/week of moderate-to-vigorous physical activity, Voorrips score <9 Exclusion Criteria: Unstable comorbidity Long term oxygen therapy or ventilation Rehabilitation program during the past 12 months nutritional supplementation during the past 4 weeks allopurinol or N-acetylcystein during the past 4 weeks long corticosteroid treatment during the past 6 months hyperreactivity to xylocain anticoagulant or platelet aggregation inhibitors treatment participation to an other research study-pregnancy or impossibility to provide informed consent, ...

Ages Eligible for Study
-----------------
Minimum Age: 40 Years
Maximum Age: 78 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: COPD Patients  90% VO2max<br>COPD Patients  90% VO2max | Procedure: Muscular biopsy<br> <br> |
| Active Comparator: COPD patients  60-65% VO2max<br>COPD patients  60-65% VO2max | Procedure: Muscular biopsy<br> <br> |
| Placebo Comparator: healthy volunteers<br>healthy volunteers | Procedure: Muscular biopsy<br> <br> Other: 5-10 weeks sport training<br> <br> |

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps |  | 5 weeks | 
| Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps |  | 10 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| quadriceps endurance |  | 5 weeks | 
| quadriceps endurance |  | 10 weeks | 
| Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps |  | 5 weeks | 
| Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps |  | 10 weeks | 
| Effect of the serum of COPD patients and SHS on pericyte morphology and in vitro |  | 10 weeks | 
| Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vitro |  | 10 weeks | 
| Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vivo |  | 10 weeks | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
COPD, skeletal muscle, angiogenesis, exercise training, intensity