Myo-inositol/D-chiro-inositol Ratio in Follicular Fluid

Study Overview
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Brief Summary
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Considering the importance assumed by follicular microenvironment for a proper oocyte development, this study is a prospective observational clinical trial which aims to evaluate whether the embryo blastocyst quality may be affected by different Myo-inositol (MI) and D-chiro-inositol (DCI) levels in the follicular fluid (FF) of the aspired oocytes. The study includes egg donors and egg receiving couples participating in the oocyte donation program. MI/DCI is calculated in FF and related with blastocyst grade.

Official Title
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Altered Myo-inositol/D-chiro-inositol Ratio in Follicular Fluid of Women Undergoing in Vitro Fertilization Has Detrimental Effects on Oocyte and Embryo Quality

Conditions
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Women Infertility

Intervention / Treatment
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* Other: Dosage of myo-inositol
* Other: Dosage of D-chiro-inositol


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: clear follicular aspirate obtained during oocyte retrieval; each specimen of follicular fluid containing only one oocyte. Exclusion Criteria: hyperglycaemic women; PCOS women also if euglycaemic; diabetic subjects having low concentrations of detectable MI in association with an anomalous MI transport.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 28 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Follicular Fluids<br>Follicular fluids obtained by 8 oocyte donors | Other: Dosage of myo-inositol<br>* Quantitative analysis of myo-inositol was performed by gas chromatography-mass spectrometry (GC-MS)<br>Other: Dosage of D-chiro-inositol<br>* Quantitative analysis of D-chiro-inositol was performed by gas chromatography-mass spectrometry (GC-MS)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Myo-inositol concentration (µmol/l) in follicular fluid | After transvaginal aspiration of oocyte and follicular fluid and subsequent ICSI, at the day 5 the cultured embryos were graded according to Gardner's grading system for blastocysts. Then follicular fluids specimens were divided following the different scores given to the respective blastocysts, and their content in myo-inositol was quantified. In each sample of follicular fluid myo-inositol levels were assessed. The quantitative analysis was performed by means of gas chromatography-mass spectrometry (GC-MS) and the values were expressed as µmol/l. This value was related to the respective blastocyst grade. | 5th day from pick up | 
| D-chiro-inositol concentration (µmol/l) in follicular fluid | After transvaginal aspiration of oocyte and follicular fluid and subsequent ICSI, at the day 5 the cultured embryos were graded according to Gardner's grading system for blastocysts. Then follicular fluids specimens were divided following the different scores given to the respective blastocysts, and their content in D-chiro-inositol was quantified. In each sample of follicular fluid D-chiro-inositol levels were assessed. The quantitative analysis was performed by means of gas chromatography-mass spectrometry (GC-MS) and the values were expressed as µmol/l. This value was related to the respective blastocyst grade. | 5th day from pick up |