Bioavailability of Plant Protein Amino Acids

Study Overview
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Brief Summary
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This study aims to inform of the mechanism of action of plant-based protein by measuring the bioavailability (i.e. rate of appearance and magnitude of concentration in the circulation following oral ingestion) of key amino acid and metabolic products that regulate skeletal muscle protein synthesis (MPS) in humans.

Detailed Description
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Plant-based proteins contain several amino acids that are essential to signal muscle growth/repair and act as a substrate during muscle protein synthesis. However, compared to animal protein or free-amino acids, the bioavailability of certain essential amino acids may be compromised. Following selective processing (e.g. removal of carbohydrate and/or other anti-nutritive factors) food grade plant-based protein concentrates possibly confer greater bioavailability and bioactivity in vivo. This project explores the bioavailability and bioactivity of selected plant-based protein concentrates following oral ingestion in young, healthy men and women.

Official Title
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Bioavailability of Amino Acids and Metabolic Products Following Oral Ingestion of Plant-Based Proteins in Young, Healthy Men and Women Aged 18-35y

Conditions
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Bioavailability of Amino Acids Following Oral Ingestion of Plant-Based Proteins in Young, Healthy Men

Intervention / Treatment
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* Dietary Supplement: Pea-protein concentrate
* Dietary Supplement: Fava-bean protein concentrate
* Dietary Supplement: Chickpea protein concentrate
* Dietary Supplement: Red lentil protein concentrate
* Dietary Supplement: Non-essential amino acid blend
* Dietary Supplement: Whey protein concentrate


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: No known intolerance to any item contained in the protein products. No blood borne disease risk, current illness or medication that would adversely affect participation No known adverse reaction to venepuncture Exclusion Criteria: n/a

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Subjects who volunteer for this study will undertake SIX controlled feeding trials, each trial separated by 7 days.Subjects will be assigned to a randomised sequence of six protein isolates including: (1) pea-protein concentrate; (2) fava-bean protein concentrate; (3) chickpea protein concentrate; (4) red lentil protein concentrate; (5) non-essential amino acid blend (negative control);( 6) whey protein concentrate. Protein is dosed at 0.33 g/kg body mass in 500 ml of water.
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Pea-protein concentrate<br>Pea-protein concentrate dosed at 0.33 g/kg body mass in 500 ml of water. | Dietary Supplement: Pea-protein concentrate<br>* Pea-protein concentrate<br>|
| Experimental: Fava-bean protein concentrate<br>Fava-bean protein concentrate dosed at 0.33 g/kg body mass in 500 ml of water. | Dietary Supplement: Fava-bean protein concentrate<br>* Fava-bean protein concentrate<br>|
| Experimental: Chickpea protein concentrate<br>Chickpea protein concentrate dosed at 0.33 g/kg body mass in 500 ml of water. | Dietary Supplement: Chickpea protein concentrate<br>* Chickpea protein concentrate<br>|
| Experimental: Red lentil protein concentrate<br>Red lentil protein concentrate dosed at 0.33 g/kg body mass in 500 ml of water. | Dietary Supplement: Red lentil protein concentrate<br>* Red lentil protein concentrate<br>|
| Experimental: Non-essential amino acid blend<br>Non-essential amino acid blend dosed at 0.33 g/kg body mass in 500 ml of water. | Dietary Supplement: Non-essential amino acid blend<br>* Non-essential amino acid blend<br>|
| Experimental: Whey protein concentrate<br>Whey protein concentrate dosed at 0.33 g/kg body mass in 500 ml of water. | Dietary Supplement: Whey protein concentrate<br>* Whey protein concentrate<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma amino acids | Plasma amino acids measured by HPLC | 3 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Bioavailability of Amino Acids Following Oral Ingestion of Plant-Based Proteins in Young, Healthy Men