Predictive Value of Maternal Serum Pentraxin 3 (PTX 3) and Heparin-binding Protein (HBP) for Chorioamnionitis in Preterm Premature Rupture of Membranes

Study Overview
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Brief Summary
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Objective: The aim of this study was to investigate the predictive value of maternal serum pentraxin 3 (PTX3) and heparin-binding protein (HBP) for chorioamnionitis in preterm premature rupture of membranes (PPROM). Method: This observational prospective cohort study included a total of 180 pregnant women at 24-40 gestational weeks. There were 60 cases of term premature rupture of membranes (TPROM), 60 cases of preterm premature rupture of membranes（PPROM）and 60 cases of healthy women. The concentrations of PTX3 and HBP were measured in maternal blood and amniotic fluid using an enzyme-linked immunosorbent assay (ELISA). Western immunoblotting was used to analyze the expression of PTX3 and HBP in placental tissue. The localization and immunoreaction of PTX3 and HBP in placenta were determined via immunohistochemistry (IHC).

Official Title
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Predictive Value of Maternal Serum Pentraxin 3 (PTX 3) and Heparin-binding Protein (HBP) for Chorioamnionitis in Preterm Premature Rupture of Membranes

Conditions
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Preterm Premature Rupture of Membrane

Intervention / Treatment
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* Other: The concentrations of PTX3 and HBP were monitored in maternal blood, placental tissue and amniotic fluid.


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The pregnant woman is born at a gestational age of 24-40 weeks; The pregnant woman has a single child; Regular obstetric examination during pregnancy with complete obstetric examination data was diagnosed as premature rupture of membranes (PROM). Exclusion Criteria: Pregnancy complicated with medical and surgical diseases, such as thyroid dysfunction and chronic hypertension; Insufficiency of vital organ function or complicated tumor; Pregnant women with other pregnancy complications besides PROM, such as preeclampsia, etc.; Neonates had major congenital malformations (congenital anal atresia, congenital biliary atresia, congenital heart disease and so on, whether prenatal suspicion or diagnosis postpartum) Neonatal septicemia, hemolytic disease or other diseases affecting bilirubin metabolism.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| healthy pregnant woman<br>healthy pregnant woman | Other: The concentrations of PTX3 and HBP were monitored in maternal blood, placental tissue and amniotic fluid.<br>* The concentrations of PTX3 and HBP were monitored in maternal blood, placental tissue and amniotic fluid.<br>|
| pregnant women with TPROM<br>pregnant women with term premature rupture of membranes | Other: The concentrations of PTX3 and HBP were monitored in maternal blood, placental tissue and amniotic fluid.<br>* The concentrations of PTX3 and HBP were monitored in maternal blood, placental tissue and amniotic fluid.<br>|
| pregnant women with PPROM<br>pregnant women with preterm premature rupture of membranes | Other: The concentrations of PTX3 and HBP were monitored in maternal blood, placental tissue and amniotic fluid.<br>* The concentrations of PTX3 and HBP were monitored in maternal blood, placental tissue and amniotic fluid.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| PTX3 and HBP concentrations in maternal blood | PTX3 and HBP concentrations in maternal blood | 24 weeks to 40 weeks gestation | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| PTX3 and HBP concentrations in placental tissue | PTX3 and HBP concentrations in placental tissue | 24 weeks to 40 weeks gestation | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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pentraxin 3, heparin-binding protein, chorioamnionitis, preterm premature rupture of membranes, term premature rupture of membranes