A Multi-omics Study of Adenomyosis

Study Overview
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Brief Summary
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This study aims to analyze the multi-omics results between eutopic endometrium, adenomyosis and endometriosis of patients diagnosed of adenomyosis with and without endometriosis. The multi-omics profiles include whole exome sequencing, analysis of transcriptomics and metabolomics. A comprehensive multi-omics will reveal the pathogenesis of adenomyosis.

Official Title
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A Multi-omics Study on the Tumorigenesis of Adenomyosis

Conditions
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Adenomyosis, Endometriosis, Ectopic Endometrium, Eutopic Endometrium, Genomics, Transcriptomics, Metabolomics

Intervention / Treatment
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* Combination Product: A multi-omics analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Confirmed adenomyosis and/or endometriosis Signed an approved informed consents Feasible for biopsy Exclusion Criteria: Not meeting all of the inclusion criteria

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group of adenomyosis<br>Patients with adenomyosis with or without endometriosis | Combination Product: A multi-omics analysis<br>* A multi-omics analysis including whole exome sequencing, transcriptomics and metabolomics for eutopic and ectopic endometrium<br>|
| Group of endometriosis<br>Patients with endometriosis without adenomyosis | Combination Product: A multi-omics analysis<br>* A multi-omics analysis including whole exome sequencing, transcriptomics and metabolomics for eutopic and ectopic endometrium<br>|
| Group of control<br>Patients without adenomyosis or endometriosis | Combination Product: A multi-omics analysis<br>* A multi-omics analysis including whole exome sequencing, transcriptomics and metabolomics for eutopic and ectopic endometrium<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Frequencies of somatic driving mutations | The differences of distributions and frequencies of somatic driving mutations will be compared between eutopic endometrium and ectopic endometrium by whole exome sequencing. | Two years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Frequencies of alteration of RNA expression | The alteration of RNA expression, including mRNA, miRNA, and lncRNA, will be compared between eutopic endometrium and ectopic endometrium by transcriptome sequencing. | Two years | 
| Frequencies of alteration of protein expression and signal pathway | The alteration of protein expression and signal pathway will be compared between eutopic endometrium and ectopic endometrium by metabolomic sequencing | Two years | 
| Progression-free survival | Progression-free survival between patients with differential expressed multi-omics will be compared. | Five years |