Estimation of Sialic Acid and IL10 Levels in Stage 1 and 2 Periodontitis Patients

Study Overview
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Brief Summary
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Sialic acid (SA) is the generic term given to a family of acetylated derivatives of neuraminic acid. SA is a 9 carbon monosaccharide. An important function of host SA is to regulate innate immunity. SA is present in several acute phase proteins which are known to be associated with periodontitis. There exists a need for a biomarker, for early detection of disease evolution and more robust therapy efficacy measurements. Till date, there is very little data regarding simultaneous estimation and comparison of total SA content in saliva. IL-10, an anti-inflammatory cytokine, regulates the synthesis of pro-inflammatory cytokines such as IL-1, -2, -6 and stimulates protective antibody production. IL-10 is expressed in both healthy and diseased human periodontal tissues and is reported to be reduced in patients infected with Aggregatibacter actinomycetemcomitans. The present study was therefore undertaken with an aim, to not only assess the levels of TSA(Total sialic acid levels),but also of IL 10 levels in saliva from stage 1(mild) to stage 2(moderate) periodontitis patients

Detailed Description
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Selection of patients by grading the patients according to criteria stage 1 and 2 Periodontitis Obtaining consent from patients participating in the study Collection of saliva-unstimulated whole saliva, the samples will be stored at -20 degree Centigrade and later placed in a 1.5ml centrifuge tubes which will be placed in a vacuum cool box indicated for laboratory samples and transported to the lab for investigations. It will be centrifuged at 2500 rpm for 10 mins,stored at -80 deg C. SA(sialic acid) levels will be determined by thiobarbutic acid method of aminoff and ELISA methods. SA levels will be measured in mg/dl,IL10 as in direct measurement.

Official Title
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Estimation of Sialic Acid and IL10 Levels in Stage 1 and 2 Periodontitis Patients

Conditions
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Periodontal Disease

Intervention / Treatment
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* Diagnostic Test: sialic acid
* Diagnostic Test: IL10


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: All the subjects should be within the age group of 18 to 55 years subject should have atleast 18 natural teeth excluding third molars. Exclusion Criteria: History of periodontal debridement or periodontal surgery in preceding six months or any antimicrobial therapy, anti-inflammatory drugs. corticosteroids, in previous three months systemically compromised individuals smokers pregnant and lactating women Medical history for absence of systemic disorders such as diabetes mellitus cardiovascular diseases pneumonia tuberculosis rheumatoid arthritis

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 55 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Diagnostic Test: sialic acid|sialic acid level estimation in salivary samples|
|Diagnostic Test: IL10|IL10 estimation in salivary samples|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Sialic acid levels in stage 1 periodontitis | Sialic acid is measured by taking salivary samples(unstimulated) from stage 1 periodontitis patients | From baseline to 3 months | 
| Sialic acid levels in stage 2 periodontitis | Sialic acid is measured by taking salivary samples(unstimulated) from stage 2 periodontitis patients | From baseline to 3 months | 
| IL10 levels in stage 1 periodontitis | IL10 is measured by taking salivary samples(unstimulated) from stage 1 periodontitis patients | From baseline to 3 months | 
| IL10 levels in stage 2 periodontitis | IL10 is measured by taking salivary samples(unstimulated) from stage 2 periodontitis patients | From baseline to 3 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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stage 1 periodontal disease, stage 2 periodontal disease, sialic acid, IL10