Biomarkers of Meats and Potatoes Intake

Study Overview
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Brief Summary
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This cross-over meal study is a four-way intervention with 12 volunteers randomized at the same time to four meals with fried meats (beef, pork, chicken and amino-acid matched non-meat control) and to four meals with potato products (boiled, chips, fries, and a control with boiled white rice) to search for biomarkers of intake.

Detailed Description
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In a two-dimensional, cross-over meal study, 12 healthy volunteers consumed in a randomized sequence four test meals: chicken, pork, beef, and a control made of egg white and pea. At the same time they were independently randomized to four starchy foods to be consumed together with the meats: boiled potatoes, fried potatoes, potato crisps or boiled white rice. Fasting and postprandial urine samples were collected to cover 48 h after each meal and samples were profiled by untargeted LC-ESI-qTOF-MS metabolomics. The profiles following the meal challenges were explored by univariate and multivariate analyses and single and combined markers of intake identified chemically by MS/MS fragmentation experiments and statistically by ROC curves and error rates as well as FDR statistics.

Official Title
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Biomarkers of Meats and Potatoes Intake: a Meal Study in Healthy Men and Women - the MEPO Study

Conditions
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Nutrition Disorders

Intervention / Treatment
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* Other: Meat arm
* Other: Starchy foods arm


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy Exclusion Criteria: non-pregnant, no abuse of alcohol or drugs, no chronic medication except p-pills

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Factorial Assignment
Interventional Model Description: Subjects were randomised to four meals with meats and starchy foods in such a way that all volunteers had each meat and each starchy food once and so that all combinations of meats and starchy foods were equally common. Each volunteer was also randomised to the sequence of the meals.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Meat meals<br>This arm contains a 4-way cross-over intervention study with meats | Other: Meat arm<br>* Randomised sequence of pork, beef, chicken meat, and an egg white/pea combination<br>|
| Experimental: Starchy meals<br>This arm contains a 4-way cross-over intervention study with | Other: Starchy foods arm<br>* Randomised sequence of boiled potato, fried potato, potato crisps, and boiled white rice<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| meat intake biomarkers | To identify intake biomarkers of general meat, red meat and poultry | 0-48 hours | 
| Potato intake biomarkers | To identify intake biomarkers of general potato, fried potato and white rice | 0-48 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Biomarkers of food intake