Proteome Remodelling

Study Overview
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Brief Summary
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The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training

Official Title
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Proteome Remodelling and Dynamics in Human Skeletal Muscle: Effect of Training

Conditions
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Skeletal Muscle Physiology

Intervention / Treatment
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* Other: Endurance training
* Other: Resistance training
* Other: Sprint training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy men and women VO2max > 55 mL/min/kgLM for men and > 50 for women (only applicable for endurance and sprint training interventions) BMI < 26 Exclusion Criteria: Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes smoking Use of prescription medicine deemed by the medical to affect study outcomes Pregnancy

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Endurance<br>Endurance training | Other: Endurance training<br>* Subjects perform a period of endurance training<br>|
| Experimental: Resistance<br>Resistance training | Other: Resistance training<br>* Subjects perform a period of resistance training<br>|
| Experimental: Sprint<br>Sprint training | Other: Sprint training<br>* Subjects perform a period of sprint training<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Skeletal muscle proteome signature | Pathway enrichment analysis of the muscle proteome | Change from baseline at week 6 or 8 depending on the intervention | 
| Anabolic signaling | Western blotting of proteins related to anabolic signaling | Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Skeletal muscle mitochondrial respiration | High resolution respirometry | Change from baseline at week 6 following endurance and sprint training | 
| Maximal oxygen consumption | VO2max during incremental exercise | Change from baseline at week 6 following endurance and sprint training | 
| Body composition | Lean and fat mass during dual X-ray absorptiometry | Change from baseline at week 6 or 8 depending on the intervention | 
| Myonuclei | Immunohistochemistry to determine number of myonuclei | Change from baseline at week 8 following resistance training | 
| Satellite cells | Immunohistochemistry to determine number of satellite cells | Change from baseline at week 8 following resistance training | 
| Fiber size | Immunohistochemistry to determine fiber size | Change from baseline at week 8 following resistance training |