Copeptin and Psychological Stress of Medic During COVID-19 Pandemic

Study Overview
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Brief Summary
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Psychological stress (PSS) is one of the most common problems among healthcare providers during COVID-19 pandemic. PSS influences the homeostatic equilibrium of the body, involving activation of the sympathetic nervous system and hypothalamus pituitary adrenal (HPA) axis. Copeptin; C-terminal portion of Vasopressin (AVP) precursor is stable. Nevertheless, Evidence about influence of PSS on copeptin levels is lacking. The reason we are doing this research is to determine the level of psychological stress among healthcare providers exposed to at the time of work in intensive care unit (ICU) during COVID-19 pandemic; They will be appraised every assembly for psychological stress level; before start of duty shifts (first time), one week after start (second time) and two weeks after departure from shift duties in ICU (third time) for assessment of psychological stress level and stress hormones.

Detailed Description
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A total of 70 healthcare personnel volunteers participated; 35 physicians and 35 nurses. All healthcare providers' volunteers are in good physical health, Exclusion criteria included hypertension, diabetes mellitus, obesity BMI ≥30, subjects with serum sodium ≤135 or ≥ 145 mmol /L at baseline or females receiving contraceptive pills. During the research participants will answer a questionnaire as well as three blood samples are taken. In the first meeting, evaluation of participant general condition; determining BMI, blood pressure. Then a small amount of blood, equal to about two millimeters, will be taken from participant arm with a syringe. This blood will be tested for serum copeptin, cortisol (fasting morning sample). The investigator will ask participant few questions to evaluate the level of stress (as anxiety, insomnia, fear of infection through questionnaire) The second meeting, one week after work in ICU, another blood sample will be taken from participant and determine level of psychological stress. The third meeting, two weeks after leave from ICU participant blood sample will be taken from participant to determine stress hormones and determine level of psychological stress. Duration The research takes place over six months in total.

Official Title
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Evaluation of Serum Copeptin and Psychological Stress Level Among Healthcare Providers During COVID-19 Pandemic

Conditions
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Psychological Stress, Hemostatic Disorder

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: physicians and nurses under age of 37 years in good health Exclusion Criteria: body mass index above 30 hypertension Diabetes mellitus females receiving contraceptive pills

Ages Eligible for Study
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Minimum Age: 24 Years
Maximum Age: 37 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| control group<br>25 healthcare personnel volunteers not working in quarantine hospitals of matched age | |
| healthcare providers worked in Intensive Care Units<br>35 physicians (28 males and 7 females) and 35 nurses (10 males and 25 females). All volunteers were in good physical health Exclusion criteria included hypertension, diabetes mellitus, obesity BMI ≥30, subjects with serum sodium ≤135 or ≥ 145 mmol /L at baseline or females receiving contraceptive pills. Assigned participants were clinically evaluated for as hypertension, DM, dyslipidemia, renal function. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| evaluation of psychological stress | - Primarily outcome determination of psychological stress among doctors and nurses working in ICU through a questionnaire before duty shifts [first time] and re-evaluate it after one week of work in ICU [second time], and lastly two weeks after departure from shift duties [third time]. | four weeks for each participant. | 
| determine stress hormones in serum cortisol and copeptin | Second to determine stress hormones copeptin and cortisol (possible stress biomarkers) concurrently with questionnaire. | four weeks for each participant.. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| correlation of psychological stress with stress hormone copeptin | correlate the level of psychological stress calculated from provided questionnaire in the three assemblies with stress biomarkers copeptin and cortisol in the three measurements. | four weeks for each participant.. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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psychological stress, copeptin, hormones