Validity and Reproducibility of Dietary Assessment Against Objective Measures

Study Overview
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Brief Summary
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A cross-sectional study with repeated measurements among healthy Danish adults to assess the validity and reproducibility of a self-administered 7-day web-based dietary assessment tool, myfood24® tested against objective biomarkers.

Detailed Description
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Healthy volunteers (age 35-70 yrs; BMI 22-32) will be recruited for the study. Informed consent will be obtained and participants will complete a self-administered web-based 7-day 24-hour dietary recall tool (myfood24®).Validity of this dietary registration tool will be assessed by comparing the estimated intake of selected nutrients (protein, energy) and selected food groups (fruit and vegetables, whole grain, coffee, alcoholic beverages) against objective biomarkers obtained from a 24-hour urine sample, through a measurement of resting energy metabolic rate, and from a fasting blood sample, respectively. Reproducibility will be assessed by comparison of the results from two self-administered web-based 7-day 24-hour dietary recalls obtained 4 weeks (± 1 weeks) apart.

Official Title
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Validity and Reproducibility of A Self-administered Web-based 24-hour Dietary Assessment Tool Among Danish Adults Against Objective Measures

Conditions
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Validation Study

Participation Criteria
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Eligibility Criteria
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Inclusion criteria: Apparently healthy adults between 35 and 70 years old Body mass index (BMI) between 22-32 kg/m2 Weight stable defined as gain or loss of < 2.5% body weight during the past 3 months; Willingness to maintain current dietary and physical activity habits throughout the study period. Have high-speed internet access; Able to speak, read and write Danish; Willing to visit the Department of Nutrition, Exercise, and Sports (NEXS). Exclusion Criteria: Clinically diagnosed chronic disease, use of corticosteroids or other medication that would potentially affect the nutrient metabolism or excretion of biomarkers of interest, presence of food allergy or intolerance that requires a special diet, pregnancy and lactation, excess physical activity (>5h/week of high intensity), current participation in another trial

Ages Eligible for Study
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Minimum Age: 35 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Validation of protein intake | Agreement between mean protein intake per day assessed by the self-administered 7-day web-based dietary assessment tool (myfood24) and nitrogen excretion in a 24-hr urine sample (urea corrected for creatine). | 7 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Validation of energy intake | Agreement between mean energy intake per day assessed by the self-administered 7-day web-based dietary assessment tool (myfood24) and resting metabolic rate (measured by ventilated hood). | 7 days | 
| Validation of vegetable and fruit intake | Agreement between mean intake of vegetable and fruit per day assessed by the self-administered 7-day web-based dietary assessment tool (myfood24) and concentration of folate in a fasting blood sample. | 7 days | 
| Validation of whole grain intake | Agreement between intake of whole grain per day assessed by the self-administered 7-day web-based dietary assessment tool (myfood24) and excretion of alkylresorcinols in a fasting blood sample. | 7 days | 
| Validation of alcohol intake | Agreement between mean alcohol intake pr day assessed by the self-administered 7-day web-based dietary assessment tool (myfood24) and excretion of ethanol and ethyl conjugates in a 24-hr urine sample. | 7 days | 
| Validation of coffee intake | Agreement between mean coffee intake per day assessed by the self-administered 7-day web-based dietary assessment tool (myfood24) and excretion of trigonelline and cyclo(isoleucylprolyl) in a 24-hr urine sample. | 7 days | 
| Reproducibility of dietary registration | Agreement between two dietary intake measurements assessed by the self-administered 7-day web-based dietary assessment tool (myfood24) obtained 4 (± 1 week) apart. | Two times 4 weeks (+/- 1 week) apart |