Study the Effect of Oral Zinc Supplementation on High Molecular Weight Zinc Binding Protein in Semen

Study Overview
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Brief Summary
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There are several causes leading to male infertility, like oxidative stress, and nutritional deficiency of trace elements like zinc. Zinc in human seminal plasma was divided into three types of ligands which are high (HMW), intermediate (IMW), and low molecular weight ligands (LMW). The present study was conducted to study the effect of Zn supplementation on the quantitative and qualitative characteristics of semen along with Zinc Binding Protein levels in semen of patients with asthenozoospmia.

Detailed Description
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Elevation of ROS levels lead to an increase the oxidation of HMW-Zn binding proteins in seminal plasma of asthenozoospermic subjects. Zinc supplementation restores HMW-Zn% in seminal plasma of asthenozoospermic subjects to normal value. LMW-Zn% is elevated in seminal plasma of asthenozoospermic patients. It may be because increment the levels of semenogelin in seminal plasma of asthenozoospermic subjects. Zinc supplementation elevates LMW-Zn% in seminal plasma of asthenozoospermic subjects to more than normal value, this may be because of its enhancement of the synthesis of metallothioneins (Low molecular weight zinc binding protein).

Official Title
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Study the Effect of Oral Zinc Supplementation on High Molecular Weight Zinc Binding Protein in Semen

Conditions
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Asthenozoospermia

Intervention / Treatment
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* Dietary Supplement: zinc sulfate


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The inclusion criteria were the presence of asthenozoospermia in the semen sample. Exclusion Criteria: the absence of endocrinopathy, varicocele, and female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels.

Ages Eligible for Study
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Minimum Age: 27 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Single group<br>Single group: all participants receive same intervention throughout study (Non-randomised) | Dietary Supplement: zinc sulfate<br>* every participant took two capsules of zinc sulfate per day for three months (each one 220mg)<br>* Other names: infertility;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| high molecular weight zinc binding protein | elevated levels of high molecular weight zinc binding protein to normal value | at the end of three months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| qualitative semen parameters | Volume of semen, progressive sperm motility percentage and total normal sperm count increased after zinc sulfate supplementation. | at the end of three months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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zinc binding protein