Evaluation of Inflammatory Markers and Periodontal Viruses on Periodontitis Patients With CAD

Study Overview
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Brief Summary
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To assess and compare the demographic variables, periodontal parameters, cardiac parameters and the expression of periodontal viruses (CMV, HSV and EBV) and inflammatory biomarkers(PTX-3, TLR-2 & TLR-4) in periodontally healthy and generalized periodontitis patients with and without coronary artery disease(CAD).

Detailed Description
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A total of 240 subjects will be recruited based on the power of the study. The selected subjects will be divided into four groups based on the inclusion and exclusion criteria. GROUP I: 60 healthy subjects ( No generalised periodontitis and no CAD) GROUP II: 60 generalised periodontitis subjects without CAD GROUP III: 60 CAD subjects without generalised periodontitis GROUP IV: 60 subjects with Generalised periodontitis and CAD Sub-gingival plaque samples will be collected from the subjects of all the four groups. Coronary artery plaque samples will be collected from the patients who were indicated for coronary artery bypass graft surgery (CABG) among group III and group IV. The demographic variables , periodontal parameters , cardiac parameters , expression of periodontal viruses and inflammatory biomarkers will be measured in all four groups ,

Official Title
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Evaluation of Demographic Variables, Clinical Parameters, Inflammatory Markers and Viruses in Periodontitis Patients With Coronary Artery Diseases

Conditions
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Periodontitis, Coronary Artery Disease

Intervention / Treatment
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* Diagnostic Test: Molecular analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients willing to participate in the study. Patients with 30 to 65 years of age group Both genders Patients should have minimum of 10 natural teeth. In case of group III and IV patients with coronary heart disease to be included. Exclusion Criteria Patients who had undergone periodontal therapy within the previous 6 months. Type I and II Diabetes mellitus Sarcoidosis patients. Carcinoma patients. Rheumatic arthritis patients. Tuberculosis patients. Immunosuppressive conditions. Patients with other inflammatory diseases.

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group I<br>Healthy group ( No generalised periodontitis and no CAD) | Diagnostic Test: Molecular analysis<br>* RT- PCR<br>|
| Group II<br>Generalised periodontitis patients without CAD | Diagnostic Test: Molecular analysis<br>* RT- PCR<br>|
| Group III<br>CAD patients without generalised periodontitis | Diagnostic Test: Molecular analysis<br>* RT- PCR<br>|
| Group IV<br>Generalised periodontitis with CAD | Diagnostic Test: Molecular analysis<br>* RT- PCR<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Periodontal Virus and Inflammatory Markers expression in periodontitis patients with coronary artery disease | Expression of EBV, CMV, HSV, Pentraxins, TLR 2 and TLR 4 using PCR | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Ebstein Barr Virus, Cytomegalovirus, Herpes Simplex Virus, Pentraxins, Toll Like Receptors