Strength Training Induced Alterations in Markers of Immune Function

Study Overview
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Brief Summary
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Exercise has been used to help prevent or slow the progression of inflammation-related disease; however, the mechanism by which this activity may lower concentrations of inflammatory markers remains unclear. The melanocortin receptors 1,3 and 5 (MC1R, MC3R and MC5R) have been shown to function in an anti-inflammatory manner and have the potential to mediate the positive immune adaptations associated with regular physical activity. Preliminary data suggest that MC3R gene expression increases in whole blood after chronic exercise training. The primary aim of the current study is to explore whether this change in gene expression translates into alterations in MC1R, MC3R, or MC5R monocyte surface expression. The secondary aim is to examine the relationship between surface expression of these receptors and circulating inflammatory profiles. The investigators will recruit 42 untrained, healthy males and females aged 18-35 yrs. Half of the group will be placed on an exercise program for 15 weeks. The other half will serve as untrained control subjects. In addition to basic anthropometric measures, the investigators will measure concentrations of inflammatory and anti-inflammatory cytokines (ELISA) and cell surface expression of MC1R, MC3R, and MC5R on monocytes (flow cytometry).

Detailed Description
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Abstract: Chronic exercise reduces inflammation, but the mechanisms involved are unclear. Recent studies have shown that stimulation of melanocortin 1 and 3 receptors (MC1R and MC3R) on immune cells increases anti-inflammatory cytokine production. PURPOSE: To examine the influence of 12 weeks of resistance training (RT) on body composition, monocyte cell-surface expression of MC1R and MC3R and circulating markers of inflammation. METHODS: Healthy, active males and females (age 20-27 yr) were recruited into a RT group (RE; n = 23) and an active control group (AC; n = 19). RE completed 12 weeks of progressive, periodized RT 3d/wk while AC maintained normal activity habits. Measures of body composition (DXA) were taken and blood was collected prior to (PRE) and following the intervention period (POST). Blood samples were analyzed for monocyte cell-surface expression MC1R, MC3R, MC5R and C-reactive protein (CRP) and the plasma cytokines interleukin 6 (IL-6) and interleukin 10 (IL-10) using flow cytometry and ELISAs respectively.

Official Title
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Strength Training Induced Alterations in Markers of Immune Function

Conditions
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Inflammation

Intervention / Treatment
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* Behavioral: Strength Training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: All Participants Must be Healthy The Strength Training Group Members Must Be Approved For Participation by a Licensed MD Exclusion Criteria: Females may not be pregnant Unhealthy participants (those with existing conditions)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Strength Training<br>The strength training group completed 12 weeks of progressive, periodized resistance training 3d/wk. | Behavioral: Strength Training<br>* Each Member of this group completed 12 weeks of progressive, periodized resistance training for 3d/wk.<br>|
| No Intervention: Active Control Group<br>The active control group was instructed to maintain normal activity and eating habits. They participated in all pre and post intervention measures. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Melanocortin 1 Receptor Expression on Monocytes | Blood samples were analyzed for monocyte cell-surface expression of the melanocortin 1 receptor using flow cytometry. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects. | Before and After 12 Week of Intervention Period | 
| Plasma CRP | Blood samples were analyzed for C-reactive protein using ELISA. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects. | Before and After the 12 Week Intervention Period | 
| Body Composition (Fat Tissue Amount) | Body composition was assessed using DXA. Pre measures were completed in Jan. 2010 and post measures were completed in May 2010 for all subjects. | Before and After the 12 Week Intervention Period | 
| Plasma IL-6 | Blood samples were analyzed for IL-6 using ELISA. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects. | Before and After the 12 Week Intervention Period | 
| Plasma IL-10 | Blood samples were analyzed for IL-10 using ELISA. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects. | Before and After the 12 Week Intervention Period | 
| Melanocortin 3 Receptor Cell Surface Expression on Monocytes | Blood samples were analyzed for monocyte cell-surface expression of the melanocortin 3 receptor using flow cytometry. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects. | Before and After the 12 Week Intervention Period | 
| Melanocortin 5 Receptor Cell Surface Expression on Monocytes | Blood samples were analyzed for monocyte cell-surface expression of the melanocortin 5 receptor using flow cytometry. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects. | Before and After the 12 Week Intevention Period | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Melanocortin Receptor, Monocyte, Resistance Training, Inflammation, Markers of Inflammation