Gene Expression Changes of Brain Tissue in Human Temporal Lobe Epilepsy With Hippocampal Sclerosis

Study Overview
=================
Brief Summary
-----------------
Epilepsy is a common neurological disease, manifested in the sudden abnormal discharge of neurons leading to short-term brain dysfunction, has become the neurology after headache the second most common disease. In China, the prevalence of epilepsy is about 4.7-8.5 per 1,000, and more than 400,000 new cases of epilepsy are developed each year. Of these, 30% of patients were treated with ineffective medication, developing into a drug-incurable epilepsy that required surgery and other treatments. The most common type of epilepsy is temporal lobe epilepsy, while the common complication in temporal lobe epilepsy is hippocampal sclerosis, which often requires surgical removal. The incidence of inner temporal lobe epilepsy associated with hippocampal sclerosis is increasing, but its exact cause and specific pathogenesis are still unclear, so clarifying its pathogenesis will contribute to the understanding of temporal lobe epilepsy and the improvement of surgical procedures. This study is intended to get single-cell transcriptome as well as spatial transcriptome data of temporal lobe and hippocampus samples. By studying gene expression change associated with epilepsy and hippocampus sclerosis, we intended to find possible prognostic-related molecules and to deepen understanding of pathological changes in epilepsy at the molecular level.

Official Title
-----------------
Gene Expression Changes of Brain Tissue in Human Temporal Lobe Epilepsy With Hippocampal Sclerosis

Conditions
-----------------
Epilepsy, Hippocampal Sclerosis

Intervention / Treatment
-----------------
* Other: RNA sequencing


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Patients confirmed in our hospital as having temporal lobe epilepsy and intending surgery to remove temporal lobe and hippocampal tissue; Volunteer to participate in this clinical trial, comply with the requirements of this clinical trial and sign an informed consent form. Exclusion Criteria: Chronic diseases of the central nervous system other than temporal lobe epilepsy; Other patients that are not suitable for this clinical trial determined by the researchers;
Sexes Eligible for Study
-----------------
All

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| epilepsy with HS<br> | Other: RNA sequencing<br>* Samples in both groups will be sequenced by single-cell sequencing and spatial transcription sequencing.<br>|
| epilepsy without HS<br> | Other: RNA sequencing<br>* Samples in both groups will be sequenced by single-cell sequencing and spatial transcription sequencing.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| RNA sequencing data | Single-cell sequencing and spatial transcription sequencing data of samples. | up to 24 weeks | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
Epilepsy, Hippocampal Sclerosis