Proteomic Analysis of Crohn's Disease Macrophages in Response or Not to AIEC

Study Overview
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Brief Summary
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The M2iSH laboratory showed with two previous clinical trials that Crohn's Disease (CD) macrophages present i) a defect to control Adherent-Invasive Escherichia coli (AIEC) infection related to polymorphisms associated with CD; ii) a CD - specific cytokine secretion profile after an AIEC infection and intestinal inflammation dependent; iii) a modification of the response of CD macrophages at a basal state and after the AIEC infection. These results consolidate the hypothesis of a defect specific to CD macrophages. That's why, the primary purpose of this study is to realize a proteomic analysis of macrophages of CD patients infected or not with AIEC and to compare them to Ulcerative Colitis (UC) patients and healthy volunteers.

Detailed Description
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The macrophages characterization will be realized at a basal state and in response or not to AIEC. Investigator aimed to 1) better understand the differences between macrophages of CD patients, UC patients and healthy volunteers but also the impact of AIEC infection on these macrophages to provide key informations to detect and characterize the defect of these macrophages; 2) highlight one or several protein in CD macrophages that could represent, in a long term, a potential therapeutic target of CD.

Official Title
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Proteomic Analysis of Crohn's Disease Macrophages in Response or Not to Adherent-Invasive Escherichia Coli

Conditions
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Crohn's Disease, Ulcerative Colitis

Intervention / Treatment
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* Other: Proteomic analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Crohn's Disease or ulcerative colitis or healthy volunteers Age > 18 years Patients benefiting from the health insurance plan Patients who can read and attest to understanding the information note and informed consent Exclusion Criteria: Pregnant or breastfeeding woman Under guardianship or curatorship Refusing the genetic part of the study

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Crohn's Disease patients group<br>44 patients | Other: Proteomic analysis<br>* Proteomic analysis of Crohn's disease macrophages in response or not to Adherent-Invasive Escherichia coli<br>|
| Ulcerative Colitis patients group<br>22 patients | Other: Proteomic analysis<br>* Proteomic analysis of Crohn's disease macrophages in response or not to Adherent-Invasive Escherichia coli<br>|
| Healthy volunteers group<br>22 patients | Other: Proteomic analysis<br>* Proteomic analysis of Crohn's disease macrophages in response or not to Adherent-Invasive Escherichia coli<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Compare the proteomic profile of macrophages of patients with CD to patients with UC and healthy subjects in the basal state | Compare concentrations of different protein types in the supernatant of macrophages from patients with CD through proteomic approaches, compared with patients with UC or controls. | at day 1 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Comparison of concentrations of different protein types in the supernatant of macrophages |  | at day 1 | 
| presence of subgroups of patients with CD based on the proteomic profile of their macrophages. |  | at day 1 | 
| association between these subgroups of patients with CD and biological parameters. |  | at day 1 | 
| associations between the proteomic profiles of macrophages of patients with CD, UC or control subjects and fecal AIEC status |  | at day 1 | 
| associations between the levels of entry, survival and multiplication of AIEC bacteria |  | at day 1 | 
| impact of anti-TNF treatment on the proteomic profile (s) observed for macrophages of patients with CD |  | at day 1 | 
| associations between the Proteomic profile (s) of the macrophages derived from monocytes and their AIEC status |  | at day 1 | 
| associations between the Proteomic profile (s) of tissue macrophages and their AIEC status |  | at day 1 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Macrophages, Crohn's Disease, Ulcerative colitis, Adherent-Invasive Escherichia coli, Proteomic analysis