Effect of Oral Zinc Sulfate Supplementation on Enzymes of Urate Pathway

Study Overview
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Brief Summary
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Although many reports have investigated the relationship between subfertility and uric acid levels in semen, to the best of author knowledge, there is no study have been published to yet focus on the effects of asthenospermia treatments, such as given an oral zinc supplementation, on the activity of urate-related enzymes, which are important in fertility of human.

Detailed Description
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The present study was designed to investigate the effect of zinc supplementation on the qualitative and quantitative characteristics of semen, along with uric acid levels and urate-related enzymes in the seminal plasma and spermatozoa of asthenospermic males.

Official Title
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Effect of Oral Zinc Sulfate Supplementation on Enzymes of Urate Pathway in Spermatozoa and Seminal Plasma of Iraqi Asthenospermic Patients

Conditions
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Infertility Unexplained

Intervention / Treatment
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* Dietary Supplement: Zinc Sulfate


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The presence of asthenozoospermia in the semen sample. Must be unable to reproduce. Exclusion Criteria: the absence of endocrinopathy, varicocele, and female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels.

Ages Eligible for Study
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Minimum Age: 26 Years
Maximum Age: 36 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: Semen volume after treatment.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Zinc sulfate<br>60 subfertile (age 32.5±3.23 year) men with asthenozoospermia was treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg). | Dietary Supplement: Zinc Sulfate<br>* The subfertile group treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg).<br>|
| No Intervention: Healthy control<br>60 fertile (age 31.6±3.3 year) men, no treatment. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurement the uric acid level in spermatozoa and seminal plasma by using spectrophotometer. | Clinical parameter | up to three months. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurement the adenosine deaminase activity in spermatozoa and seminal plasma by using spectrophotometer. | Enzyme | up to three months. | 
| Measurement the xanthine oxidase activity in spermatozoa and seminal plasma by using spectrophotometer. | Enzyme | up to three months. | 
| Measurement the 5'-nucleotidase activity in spermatozoa and seminal plasmaby using the spectrophotometer. | Enzyme | up to three months. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Uric acid, Adenosine deaminase, Zinc Sulfate, 5'-nucleotidase, Asthenospermia., Xanthine oxidase