The Role of Endothelial Dysfunction in Adult Polycystic Kidney Disease

Study Overview
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Brief Summary
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This pilot study will compare endothelial function in patients with ADPKD with matched healthy volunteers and normotensive chronic kidney disease stage 1 & 2 patients. Patients will undergo a single assessment of endothelial function and measurement of plasma and urine levels of biomarkers of endothelial function.

Detailed Description
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Following recruitment we will record the following baseline demographics; age, gender, smoking status, body mass index, medical history. Participants will undergo a noninvasive vascular assessment, venepuncture and will provide a urine sample.This will be done during a single visit to the CRF. Noninvasive vascular assessment will consist of: Peripheral Arterial Tonometry This will be measured using the EndoPAT system (Itamar Medical) Endothelial shear stress flow stimulus will be provided by inflation of a blood pressure cuff around the upper non-dominant arm to suprasystolic pressure (but <300mmHg) for 5 minutes. Following release of the cuff the resulting digital microcirculation vasodilatory response to ischaemic hyperaemia, quantified as the pulse wave amplitude, will be monitored over 5 minutes. The peak pulse wave amplitude measured at 90-150s post cuff deflation relative to baseline will be indexed to the changes in the opposite arm to give the reactive hyperaemia index. This measure of endothelial function constitutes the outcome measure. Blood Pressure Assessment Brachial artery blood pressure will be measured in the left arm in the supine position using an automated device (Dinamap) and in accordance with the clinical research facility SOP. Blood samples and urine samples Samples for plasma will be centrifuged and stored at -80C. Biochemical analysis will be performed by the laboratory of the STH NHS Trust for standard tests and by validated assays for non-routine markers (as detailed below) in the Academic Nephrology Unit. Participants will be asked to collect a 24h urine sample from the day before the study visit and to provide a spot urine sample on the day. This will be analysed at the laboratory of the STH NHS Trust. Biochemical Analysis Serum samples will be analyzed for Full blood count, Urea, Electrolytes, Glucose, Insulin, Lipid Profile, Homocysteine, (Hs) CRP and Creatinine Levels. Serum and plasma will also be stored for future analysis of relevant biomarkers of endothelial function or nitric oxide metabolism as indicated by preliminary results. Urine samples will be analysed initially for protein creatinine ratio (PCR). The rest will be frozen for future analysis of urinary biomarkers of endothelial function or nitric oxide metabolism.

Official Title
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Endothelial Dysfunction in Autosomal Dominant Polycystic Kidney Disease

Conditions
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Polycystic Kidney, Autosomal Dominant

Intervention / Treatment
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* Diagnostic Test: Peripheral (digital) arterial tonometry


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age >18yrs, <50yrs Able to understand spoken/written English sufficiently to give informed consent and to take part in clinical assessment Clinical diagnosis of ADPKD eGFR >60ml/min/1.73m2 Exclusion Criteria: No history of cardiovascular disease or hypertension Not diabetic Not smoking Not breastfeeding Not pregnant Not taking any regular medication (except oral contraceptives) No musculoskeletal conditions contraindicating inflation of a blood pressure cuff to suprasystolic pressures around the forearm (as part of EndoPAT measurements) Not morbidly obese: BMI<35 (May contribute to endothelial dysfunction) eGFR <60ml/min/1.73m2

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| ADPKD<br>ADPKD patients | Diagnostic Test: Peripheral (digital) arterial tonometry<br>* This will be measured using the EndoPAT system (Itamar Medical)<br>|
| Controls<br>Healthy volunteers | Diagnostic Test: Peripheral (digital) arterial tonometry<br>* This will be measured using the EndoPAT system (Itamar Medical)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Reactive hyperemia index (RHI) | EndoPAT values (Endoscore) | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Systolic and diastolic Blood pressure | mm Hg | Baseline | 
| Proteinuria | Protein creatinine ratio | Baseline |