The Association of Transgelin-2, Metallothionein-2 and Ezrin With Asthma

Study Overview
=================
Brief Summary
-----------------
Asthma is one of the most common chronic respiratory diseases. Previous study found a series of differentially expressed genes and proteins (transgelin-2, metallothionein-2, ezrin) in asthmatic rat. The aim of the study is to investigate the changes and associations of these gene with asthma in human.

Detailed Description
-----------------
This study will investigate the changes and associations in asthma. In this project, the investigators will explore the changes of gene expression of transgelin-2, metallothionein-2 and ezrin in asthma and investigate their correlation with current diagnosis markers of asthma, including pulmonary function, fractional exhaled nitric oxide,eosinophil counts,immunoglobulin E, interleukin-5, etc.

Official Title
-----------------
The Association of Transgelin-2, Metallothionein-2 and Ezrin With Asthma: A Case-control Study

Conditions
-----------------
Asthma

Intervention / Treatment
-----------------
* Diagnostic Test: No intervention


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Males or females, aged 14-75 years; Patients with a definite diagnosis of bronchial asthma; The diagnosis of asthma according to the Global Initiative for Asthma (GINA), with positive bronchodilator responsiveness (reversibility) test; Healthy volunteers without respiratory symptoms such as cough, wheezing, chest tightness or dyspnea; Agree with all procedures in this trial by signing a written informed consent form. Exclusion Criteria: Patients with chronic obstructive pulmonary disease or other airway disease; Complicated with other severe primary diseases (including hypertension, cancer, hyperthyroidism, bronchiectasia, cardiac insufficiency) and conditions that would prevent participation in the trial or put the participant at risk; Women who are known to be pregnant or breastfeeding; Not willing to participate; Psychiatric history and neurological disorders can not work normally; Other conditions that the researcher considers inappropriate to participate in this study.

Ages Eligible for Study
-----------------
Minimum Age: 14 Years
Maximum Age: 75 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Asthma<br>Previously disgnosed asthma patients | Diagnostic Test: No intervention<br>* No intervention<br>|
| Control<br>Healthy participants | Diagnostic Test: No intervention<br>* No intervention<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Difference in gene expression of transgelin-2, metallothionein-2 and ezrin between controls and asthmatic patients | PCR test of gene expression of transgelin-2, metallothionein-2 and ezrin between controls and asthmatic patients | Two year | 
| Association of transgelin-2, metallothionein-2 and ezrin with asthma | Correlation analysis between gene expressions of transgelin-2, metallothionein-2, ezrin and current asthmatic tests (pulmonary function, fractional exhaled nitric oxide,immunoglobulin E, serum eosinophil counts, interleukin-5, etc) | Two year |