Preliminary Study to the Manufacturing of a Non-stick Membrane for Surgery Using Patient's Own Cells (MESO-PATCH)

Study Overview
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Brief Summary
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The objective of this study, which will be realised in vitro on human samples, is to test 2 cell sources isolated from peritoneum for adult mesothelial cells (peritoneal samples) and from adipose tissue for the adipose stem cells (adipose tissue samples). The latter will be differentiated towards the mesothelial phenotype and morphotype. The 2 routes will be compared and the most satisfactory one will be chosen to make a non-stick patch.

Detailed Description
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Patient information and collection of a signed informed consent Clinical data collection: surgery date, age, weight, height, medical and surgical history Prospective collection of peritoneal and adipose biopsies of patients during their surgery at the Oscar Lambret Center (Dr L. BRESSON) Transportation of the samples to the 1008 INSERM Unit. Isolation of cells from the samples : mesothelial cells from the peritoneum and adipose stem cells from adipose tissue Cell culture and expansion of mesothelial cells; and cell culture and differentiation of adipose stem cells Identification by the morphotype and phenotype of mesothelial cells Comparison of the results according to the cell source, peritoneum or adipocyte, to the purity of cells and to the efficacy of the expansion

Official Title
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Non-stick Patch of Mesothelial Cells in a Hydrogel Matrix : Exploratory Analysis Assessing the Morphotype and Mesothelial Phenotype After Culture From 2 Cells Sources of Human Origin.

Conditions
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Cancer

Intervention / Treatment
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* Procedure: Biological samples collection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Aged more than 18 years old, Planned surgery For peritoneal samples : abdominal surgery (laparotomy or coelioscopy) For adipose tissue samples : breast reconstructive surgery (lipomodelage) Informed and signed consent Exclusion Criteria: Pregnant or breastfeeding woman Patient under guardianship or tutorship For peritoneal samples : previous abdomino-pelvic radiotherapy or abdominal surgery ; peritoneal carcinosis

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Biological samples collection<br>-Peritoneal samples : will be collected during abdominal surgery A biopsy from 1 to 2 cm2 will be performed in healthy peritoneum -Adipose tissue samples : will be collected during surgery for breast reconstruction | Procedure: Biological samples collection<br>* -Peritoneal samples : will be collected during abdominal surgery A biopsy from 1 to 2 cm2 will be performed in healthy peritoneum - Adipose tissue samples : will be collected during surgery for breast reconstruction<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Morphotype/Phenotype expression | Mesothelial morphotype and phenotype expression of the 2 types of cells after culture | 1 year | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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reconstructive surgery, abdominal surgery