Fluid Balance, Hormones and Urine Proteomics in Nephrotic Syndrome in Childhood

Study Overview
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Brief Summary
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The purpose of this study is to describe the hormones controlling fluid balance in pediatric patients with nephrotic syndrome. Further more, an analysis of the urinary and plasma proteins will be done using proteomics. Different composition of proteins in the urine or plasma might indicate if the patients will respond to treatment or not.

Detailed Description
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Nephrotic syndrome represents the association of proteinuria, hypoalbuminemia, oedema and hyperlipidemia. The pathogenesis of the oedemas remains controversial. The underfill theory is the traditional explanation where massive proteinuria leads to low plasma albumine and a subsequent decrease in intravascular osmotic pressure leading to edema formations. Because most patients are normotensive and have normal intravascular pressure the overfill theory has been proposed suggesting a primary defect in renal sodium handling being responsible for oedema formation. Ten percent of the children with nephrotic syndrome do not respond to standard steroid treatment and a significant proportion of these patients progress towards end-stage renal failure. At initial presentation it cannot be said if a patient will respond to treatment or not. The purposes of the sudy: To describe changes in the hormones, Aldosterone, Atrial Natriuretic Peptide (ANP), Arginin Vasopressin (AVP), Renin and Angiotensin II in patients with idiopathic nephrotic syndrome and to analyse to what extend the change in these hormones reflected a underfill or overfill situation. To describe changes in the urine concentration of the water channel AQP II and the sodium channel ENaC during the course of nephrotic syndrome. To test the hypothesis that urine and plasma proteomics from patients with steroid resistant nephrotic syndrome differs from patients with steroid sensitive nephrotic syndrome. 50 pediatric patients with nephrotic syndrome will be included after informed consent from both parents. At day 1, 2, 3, 30 and 120 blood samples for hormones will be taken together with creatinin, albumine, Na+, K+, Hgb. An Echocardiography will be performed at day 1 and day 30 to determine v. cava inferior index. At day 1,30 and 120 urine and plasma will be collected for proteomics and measurement of AQP II and ENaC concentrations. Further more a clinical examination will be performed at day 1, 2, 3, 30 and 120 and weight, blood pressure and response to treatment will be recorded.

Official Title
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Fluid Balance, Hormones and Urine Proteomics in Nephrotic Syndrome in Childhood

Conditions
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Nephrotic Syndrome

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Idiopathic Nephrotic syndrome, age below 15 years Exclusion Criteria: Systemic disease e.g. Henoch-Schonleins purpura, Lupus erythematosus, diabetes mellitus Age over 15 years Treatment with diuretics prior to baseline blood samples

Ages Eligible for Study
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Minimum Age: 1 Year
Maximum Age: 15 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Nephrotic syndrome, Underfill and overfill theory, Steroid resistance, Urine proteomics, AVP, ANP, Angiotensin II, Renin and Aldosterone