Garlic With Lime Juice and Chlorhexidine Mouthwash

Study Overview
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Brief Summary
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the study about herbal mouthwash. it is clinical trial .the investigator will compare between chlorhexidine mouthwash and garlic with lime juice mouthwash.and their effect on gingival bleeding and plaque and salivary bacteria.

Detailed Description
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the study about herbal mouth wash. it is clinical trial .the investigator will compare between chlorhexidine mouthwash and garlic with lime juice mouthwash.and show their effect on gingival bleeding and strepococcus bacteria present in saliva and plaque. Preparation of garlic with lime juice will occur under aseptic condition.and give it to the first group. provide chlorhexidine to the second group. Collection of plaque and saliva samples will be obtained from children who fulfilled the inclusion criteria. Asses gingival condition by asking patient. Subjects will be instructed to rinse for 14 days, twice daily. Morning after breakfast and night before going to bed. With 10 ml (undiluted) of the assigned mouthwash for 30 s and then expectorate the rinse. Again after 14 days, the saliva and plaque samples will be taken and inoculated on blood agar plates to determine the colony count and the gingival index by loe and illness will be assessed.

Official Title
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Evaluation of Garlic With Lime Juice and Chlorhexidine Mouthwash on Gingival Bleeding in Group of Egyptian Children: A Randomized Clinical Trial

Conditions
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Gingival Bleeding

Intervention / Treatment
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* Drug: garlic with lime mouth wash
* Drug: chlorhexidine


Participation Criteria
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Eligibility Criteria
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Inclusion criteria: Children aged between 9 and 12 years. Children are having normal occlusion (no crowding), non-compromised oral health (brushed their teeth once-daily) using toothbrush and non-fluoridated toothpaste. And practicing no other oral hygiene measures. Medically free history. Absence of orthodontic appliances. Exclusion criteria: History of current or recent (at least for the past 1 month) antibiotic usage or other medications. Presence of Abscess, draining sinus, cellulitis, or other conditions requiring emergency dental treatment. not willing to comply with the study.

Ages Eligible for Study
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Minimum Age: 9 Years
Maximum Age: 12 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: A Randomized Clinical Trial parallel
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: garlic with lime juice mouthwash<br>garlic with lime juice mouthwash is herbal antimicrobial .children were instructed to rinse for 14 days, twice daily, ,10 ml (undiluted) for 30 second and then expectorate . To prepare garlic with lime mouth rinse, 100 g of fresh, washed garlic cloves was macerated in a sterile, ceramic mortar and water was added to obtain a homogenate which was then filtered off with a sterile muslin cloth. the final concentration of the solution was determined to be 1 g/100 ml. About 100 ml of lime juice was extracted from fresh lemons using a juice extractor and added to the garlic extract. | Drug: garlic with lime mouth wash<br>* garlic with lime juice is herbal antimicrobial 10 ml (undiluted) for 30s and then expectorate for 14 days.o prepare garlic with lime mouth rinse, 100 g of fresh, washed garlic cloves was macerated in a sterile, ceramic mortar and water was added to obtain a homogenate which was then filtered off with a sterile muslin cloth. the final concentration of the solution was determined to be 1 g/100 ml. About 100 ml of lime juice was extracted from fresh lemons using a juice extractor and added to the garlic extract.<br>* Other names: herbal mouthwash;|
| Active Comparator: chlorhexidine mouthwash<br>chlorhexidine is antimicrobial, antiplaque mouthwash 10 ml (undiluted) for 30 second twice daily and then expectorate for 14 days. it is commercial mouthwash . | Drug: chlorhexidine<br>* hlorhexidine is antimicrobial, antiplaque mouthwash 10 ml (undiluted) for 30 second twice daily and then expectorate for 14 days.<br>* Other names: antimicrobial mouthwash;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| gingival bleading | bleeding of gingiva asses by yes or no | 15 day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Streptococcal count in saliva and plaque | Streptococcal count in saliva and plaque | 15 day |