Plasma Biomarkers of Cardiopulmonary Bypass Induced Acute Respiratory Distress Syndrome (CPB-ARDS)

Study Overview
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Brief Summary
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The investigators aimed to establish a prospective cohort of patients undergoing cardiopulmonary bypass (CPB) in cardiac surgery from April 2021 to September 2022, in Wuhan. The ARDS events, ventilation time, time of extubation, oxygenation index for 3 days after operation were observed. Plasma samples were collected before CPB, and several time points after CPB. Dynamic differential proteins of ARDS after CPB were screened by DIA (Data independent acquisition) proteomics. Quantitative protein marker concentration was used to predict the occurrence of ARDS after operation, the model discrimination and calibration was assessed.

Official Title
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Explore Potential Plasma Biomarkers of Acute Respiratory Distress Syndrome (ARDS) Using Proteomics Analysis in Patients Undergoing Cardiopulmonary Bypass

Conditions
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Acute Respiratory Distress Syndrome

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Selective operation; Cardiac surgery with cardiopulmonary bypass; Adult patients over 18 years old; Signed informed consent Exclusion Criteria: patients who refuse to sign the informed consent form or the attending physician refuses the patient to join the study; non elective surgery (surgery at non-elective time or emergency surgery); preoperative pulmonary insufficiency, pulmonary hypertension and pulmonary inflammation; the absence of any specimen and clinical data; patients who failed the operation, needed extracorporeal membrane oxygenation support or underwent CPB operation again within 3 days after operation;

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients undergoing cardiopulmonary bypass (CPB) with postoperative ARDS<br> | |
| Patients undergoing cardiopulmonary bypass (CPB) without postoperative ARDS<br> | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of participants with postoperative ARDS events as assessed by Berlin Definition. | The Berlin Definition of Acute Respiratory Distress Syndrome: Timing: Within 1 week of a known clinical insult or new or worsening respiratory symptoms; Chest imaging: Bilateral opacities-not fully explained by effusions, lobar/lung collapse, or nodules; Origin of edema: Respiratory failure not fully explained by cardiac failure or fluid overload. Need objective assessment (eg, echocardiography) to exclude hydrostatic edema if no risk factor present; Oxygenation: Mild 200 mm Hg <PaO2/FIO2≤ 300 mm Hg with PEEP or CPAP ≥5 cm H2O; Moderate 100 mm Hg <PaO2/FIO2≤ 200 mm Hg with PEEP ≥5 cm H2O Severe PaO2/FIO2≤ 100 mm Hg with PEEP ≥5 cm H2O | 3 days after operation | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Acute Respiratory Distress Syndrome, Biomarkers, Proteomics, Cardiopulmonary Bypass