Biomarkers of Atrial Fibrillation Complicated With Stroke Based on Proteomics and Metabolomics

Study Overview
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Brief Summary
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Thrombus shedding in patients with atrial fibrillation (AF) can lead to cerebral artery embolism. Stroke caused by AF is very dangerous, which not only threatens the life of patients, but also seriously affects the quality of life. This study aims to explore the biomarkers of stroke in patients with AF by integrating proteomics and metabolomics data, and establish the network relationship of stroke in patients with AF, so as to reveal the molecular mechanism of stroke in patients with AF.

Official Title
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Biomarkers of Atrial Fibrillation Complicated With Stroke Based on Proteomics and Metabolomics

Conditions
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Atrial Fibrillation, Stroke

Intervention / Treatment
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* Diagnostic Test: Proteomics
* Diagnostic Test: Metabolomics


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 18 years or above Admission with atrial fibrillation or clinic visit for atrial fibrillation Receive routine anticoagulant therapy; Signing the consent form Exclusion Criteria: Pregnant women; Lactating women; Severe mitral stenosis; Severe impairment of liver function; Severe renal insufficiency; Thyroid dysfunction requiring treatment.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| AF<br>Patients with AF | Diagnostic Test: Proteomics<br>* Proteomic data were obtained by LC-MS/MS Analysis-DIA platform.<br>Diagnostic Test: Metabolomics<br>* Metabolomic data were obtained by UPLC-QTOF/MS platform.<br>|
| AF and stroke<br>Patients with AF complicated with stroke | Diagnostic Test: Proteomics<br>* Proteomic data were obtained by LC-MS/MS Analysis-DIA platform.<br>Diagnostic Test: Metabolomics<br>* Metabolomic data were obtained by UPLC-QTOF/MS platform.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Biomarkers of atrial fibrillation complicated with stroke based on proteomics and metabolomics | By combining proteomics and metabolomics data, the investigators were able to identify the common pathways involved in AF with stroke. | 1 day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Quantitative changes of individual metabolites and proteins were calculated and graphed using the KEGG mapping tools. |  | 1 day | 
| By combining proteomics and metabolomics data, the investigators were able to identify the common pathways involved in AF with stroke. |  | 1 day | 
| All of the omics data were used to compare proteins/enzymes with metabolic pathways. |  | 1 day | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Atrial Fibrillation, Stroke, metabolomics, proteomics, Biomarkers