Effect of Passive Ultrasonic Irrigation on Bacteria That Persist After Root Canal Preparation

Study Overview
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Brief Summary
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This study aims to evaluate the effectiveness of ultrasonic irrigation in improving root canal disinfection after chemomechanical procedures.

Detailed Description
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Although chemomechanical procedures promote a drastic bacterial reduction, many root canals remain infected, which points to the need for complementary procedures to improve root canal disinfection. Thus, this clinical study aims to evaluate the effect of passive ultrasonic irrigation as a supplementary disinfection procedure after root canal preparation. Microbiological samples of the root canals of 30 single-rooted teeth with apical periodontitis will be taken at different stages of the endodontic treatment: before preparation (S1), after chemomechanical procedures (S2) and after passive ultrasonic irrigation (S3). The samples will be submitted to the quantitative polymerase chain reaction for bacterial quantification and to the reverse transcription quantitative polymerase chain reaction for bacterial activity analysis. Data will be analyzed using the Wilcoxon test for paired samples (p < 0.05).

Official Title
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Effect of Passive Ultrasonic Irrigation on Bacteria That Persist After Root Canal Preparation in Teeth With Apical Periodontitis

Conditions
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Bacterial Infections

Intervention / Treatment
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* Procedure: Ultrasonic irrigation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Teeth with necrotic pulps and asymptomatic apical periodontitis Exclusion Criteria: patients who had received antibiotics during the previous 3 months or had any general disease, teeth that could not be properly isolated with rubber dam, non-restored teeth, periodontal pockets depths greater than 4 mm, previous endodontic treatment, open apex, crown/root fracture root resorption or calcifications.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Microbiological analysis of root canals after endodontic procedures<br>Bacterial levels and activity in root canal samples after chemomechanical procedures and ultrasonic irrigation. | Procedure: Ultrasonic irrigation<br>* Ultrasonic irrigation will be used as a complementary step to chemomechanical procedures to test whether it can improve root canal disinfection.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Bacterial Activity | The metabolic activity of total bacteria in root canal samples after ultrasonic irrigation | through study completion, an average of 3 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Bacterial Levels | Quantitative data of total bacteria determined by quantitative polymerase chain reaction after endodontic procedures | through study completion, an average of 3 years |