Effects of Faba Bean Fractions as Ingredients in Novel Food Products on Glycemia, Appetite and Metabolic Control

Study Overview
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Brief Summary
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To describe the effect and mechanisms of action faba bean powder and fractions (protein, starch and fiber) on glucose and insulin homeostasis, food intake, satiety and metabolic regulation in young adults (18 to 30 years).

Detailed Description
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The overall objective is to describe the effect and mechanisms of action of faba bean powder and fractions (protein, starch and fiber) on glucose and insulin homeostasis, food intake, satiety and metabolic regulation in young adults (18 to 30 years). The specific objectives are to test the acute effects of different faba bean ingredient smoothie on: 1) post-prandial blood glucose, appetite, satiety hormone and insulin for 200 minutes, and 2) food intake 120 minutes following consumption of the faba bean smoothie. Two short-term acute studies will be conducted to meet these objectives. Both acute studies will follow a randomized, double blinded, balanced, repeated measures design and the same protocol; except one will have an ad libitum pizza meal (study 1), while the other will have a fixed pizza meal (study 2) at 120 minutes. Half the participants will have blood drawn by IV and the other half will be finger prick.

Official Title
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Effects of Faba Bean Fractions as Ingredients in Novel Food Products on Glycemia, Appetite and Metabolic Control

Conditions
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Faba Bean, Post-prandial Blood Glucose, Appetite, Satiety Hormones, Insulin

Intervention / Treatment
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* Other: Faba bean powder smoothie
* Other: Faba bean protein concentrate smoothie
* Other: Faba bean starch smoothie
* Other: Faba bean protein isolate smoothie
* Other: Non-pulse smoothie smoothie


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy non-smoking males aged 20-30 years (min/max age) a body mass index between 20 and 25 kg/m2 (the range generally accepted as ideal/healthy by Health and Welfare Canada) Exclusion Criteria: individuals diagnosed with diabetes those on medication breakfast skippers on an energy restricted diet this study excludes females because appetite and blood glucose during the female menstruation cycle changes due to fluctuations in hormones

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Control smoothie<br>Control smoothie with 32 g corn maltodextrin without added faba bean fractions. | Other: Faba bean powder smoothie<br>* Breakfast smoothie with 32 g whole faba bean powder (300g).<br>Other: Faba bean protein concentrate smoothie<br>* Breakfast smoothie with 32 g faba bean protein concentrate.<br>Other: Faba bean starch smoothie<br>* Breakfast smoothie with 33 g faba bean starch.<br>Other: Faba bean protein isolate smoothie<br>* Breakfast smoothie with 32 g faba bean protein isolate.<br>|
| Experimental: Faba Bean powder smoothie<br>Breakfast smoothie with 32 g whole faba bean powder. | Other: Faba bean protein concentrate smoothie<br>* Breakfast smoothie with 32 g faba bean protein concentrate.<br>Other: Faba bean starch smoothie<br>* Breakfast smoothie with 33 g faba bean starch.<br>Other: Faba bean protein isolate smoothie<br>* Breakfast smoothie with 32 g faba bean protein isolate.<br>Other: Non-pulse smoothie smoothie<br>* Control smoothie with 32 g corn maltodextrin without added faba bean fractions.<br>|
| Experimental: Faba bean protein concentrate smoothie<br>Breakfast smoothie with 32 g faba bean protein concentrate. | Other: Faba bean powder smoothie<br>* Breakfast smoothie with 32 g whole faba bean powder (300g).<br>Other: Faba bean starch smoothie<br>* Breakfast smoothie with 33 g faba bean starch.<br>Other: Faba bean protein isolate smoothie<br>* Breakfast smoothie with 32 g faba bean protein isolate.<br>Other: Non-pulse smoothie smoothie<br>* Control smoothie with 32 g corn maltodextrin without added faba bean fractions.<br>|
| Experimental: Faba bean starch smoothie<br>Breakfast smoothie with 33 g faba bean starch. | Other: Faba bean powder smoothie<br>* Breakfast smoothie with 32 g whole faba bean powder (300g).<br>Other: Faba bean protein concentrate smoothie<br>* Breakfast smoothie with 32 g faba bean protein concentrate.<br>Other: Faba bean protein isolate smoothie<br>* Breakfast smoothie with 32 g faba bean protein isolate.<br>Other: Non-pulse smoothie smoothie<br>* Control smoothie with 32 g corn maltodextrin without added faba bean fractions.<br>|
| Experimental: Faba bean protein isolate smoothie<br>Breakfast smoothie with 32 g faba bean protein isolate. | Other: Faba bean powder smoothie<br>* Breakfast smoothie with 32 g whole faba bean powder (300g).<br>Other: Faba bean protein concentrate smoothie<br>* Breakfast smoothie with 32 g faba bean protein concentrate.<br>Other: Faba bean starch smoothie<br>* Breakfast smoothie with 33 g faba bean starch.<br>Other: Non-pulse smoothie smoothie<br>* Control smoothie with 32 g corn maltodextrin without added faba bean fractions.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| blood glucose | measured by finger prick blood samples by glucose meters | 0-200 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| appetite | measured by VAS questionnaire | 0-200 minutes | 
| satiety hormones | measured by enzyme-linked immunosorbent assay (ELISA) | 0- 200mins | 
| insulin | measured by ELISA | 0-200mins | 
| food intake | energy intake at ad-libitum pizza meal | 120 minutes following consumption of the faba bean smoothie | 
| physical comfort | measured by VAS questionnaire | 0-200 minutes | 
| energy/fatigue | measured by VAS questionaire | 0-200 minutes |