A Comparison Between Conventional Surgery and Piezosurgery

Study Overview
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Brief Summary
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Evaluation of the effect of conventional and piezo-surgery on the secretion of Heat Shock Protein (HSP70) and on the vial bone cells at the peripheral bone cut sides after the removal of impacted mandibular third molars on both sides of the jaw.

Detailed Description
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The study is about measuring the amount of HSP70 using Elisa kit for detecting HSP70 and mason trichrome for histological findings in which the study samples are patients who have bilateral impacted mandibular third molar to be extracted with bone removal. First we collect bone sample for mason trichrome with either method then we collect the second samples after removing most of the bones cover the impacted third molar for checking HSP70 levels. Then the bone samples are treated using a special protocol to eliminate the protein from bone samples for the Elisa analysis. The other bone samples are going to be studied under the microscope.

Official Title
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A Histo-immunological Comparative Study of Bone Cutting by Conventional Surgery and Piezosurgery on the Secretion of Heat Shock Protein 70 (HSP70) and on the Bone Cells

Conditions
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Third Molars Extraction

Intervention / Treatment
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* Device: Piezosurgery


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: bilateral identical impacted third molar that need at least 3 mm of bone removal Exclusion Criteria: Pregnancy Smokers Infectious diseases Infections around third molars (i.e. pericoronitis). Alcoholic patients

Ages Eligible for Study
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Minimum Age: 16 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: Conventional Surgery<br>No intervention in this arm. The patients are going to undergo normal surgical intervention. Conventional surgery will be performed to extract the impacted third molars. | |
| Experimental: Piezosurgery<br>Patients here will undergo a piezosurgery to extract their third molars on both sides. | Device: Piezosurgery<br>* This intervention is dependent on use of piezosurgery to conduct the required bone cuts to remove third molars.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Levels of Heat Shock Protein 70 | This will be measured using ELISA methodology | Five minutes before third molar removal with the last removed layer of bone | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Percentage of viable bone cells | Percentage of viable bone cells will be calculated based on the counts of viable bone cells observed under the microscope. | This variable will be measured from the specimens taken within the first 15 minutes of bone cutting to remove third molars |