Analysis of Lipid Profiles in Samples Sperm and Seminal Plasma Under Artificial Insemination

Study Overview
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Brief Summary
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Identify, by Lipidomics approaches, biochemical markers of fertility / infertility in sperm and seminal plasma linking the success or failure of the artificial insemination (IUI)

Detailed Description
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Analyze lipid profiles in samples of sperm and seminal plasma of three populations: male sperm donors men assigned to a program of assisted reproduction with normal sperm / slightly abnormal successfully in the IUI; attached to a program of assisted reproduction with male semen Normal / slightly abnormal unsuccessfully in IUI Establish partnerships between the composition of fatty acids, especially fatty acids polyunsaturated (PUFA) of the omega-3 (w3) series of samples analyzed parameterssemen and results of the IA to identify biochemical markers of fertility. Assess markers found by checking the increase in the rate of success in the IA.

Official Title
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Analysis of Lipid Profiles in Samples Sperm and Seminal Plasma Under Artificial Insemination

Conditions
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Primary Infertility

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria Group A women donor group Fallopian unobstructed Men donor group Appear as sperm donor Age between 18-35 years Sexual abstinence 3-5 days Exclusion Criteria Group A: Uterine malformations (fibroids, adenomyosis, uterine malformations or acquired congenital) and fallopian tubes (tubal occlusion) Obesity BMI (Body Mass Index)> 30 kg / m2 Presence of altered tubal toxic habits (smoking> 15 cigarettes per day, alcohol or drug abuse). Inclusión / Exclusion Criteria Group B and C: ≤ 38 years Natural cycles and stimulated The criteria inclusion / exclusion group A Time sterility not exceeding three years.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 38 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Male donor<br>42 samples of semen from male donor attached to a donation program Normal sperm count as WHO 2010. | |
| IUI positive pregnancy<br>42 samples of semen from a program attached to patients for insemination Male indication Normal / slightly abnormal sperm count or infertility source unknown and have obtained positive pregnancy in any of the first 4 treatments IUI | |
| IUI unsuccessful pregnancy<br>42 samples of semen from a program attached to patients for insemination Male indication Normal / slightly abnormal sperm count or infertility source unknown and after undergoing maximum 4 cycles have unsuccessfully IUI indication of IVF / ICSI. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Identify, by Lipidomics approaches, biochemical markers of fertility / infertility sperm and seminal plasma linking the success or failure of the artificial insemination | Levels of docosahexaenoic acid (DHA) and acid eicosapentaenoic (EPA) in 42 semen samples: With positive pregnancy in any of the first 4 treatments of artificial insemination. Gestacion Negative in artificial insemination Assigned to the program-donors donation | 18 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Descriptive analysis of semen parameters versus DHA and EPA levels | Correlation analysis of seminal variables: sperm count, motility and morphology percent compared to the levels of DHA and EPA obtained. Assuming normal distribution continuous variables, ANOVA test apply. If the normality assumption failed an Kruskal-Wallis test was applied. | 18 months |