Endothelium Dependent and Non-dependent Vasodilatation in Human Gingiva

Study Overview
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Brief Summary
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The aim of this study is to compare the endothelim-dependent and non-dependent vasodilatation between genders in the human gingiva.

Detailed Description
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Gender differences were observed in studies according to gingival microcirculation between physiological circumstances and during wound healing as well. For blood flow measurement Laser Spackle Contrast Imaging is a reliable tool. It has good reproducibility, it doesn't get in touch with the measured surface, it's easy to use and it can measure on a bigger surface in same time. Two wells were fabricated on the labial surface of the right lateral incisor (test tooth) and on the labial surface of the left central incisor (control tooth). These wells are opened towards the high-prermeability gingival sulcus, so the dropped solutions nitroglycerine vs physiological saline or nitroglycerine vs physiological saline can esasily penetrate to the gingival tissues. After applying the solutions, the blood flow was monitored fot 15 minutes.

Official Title
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Investigation of the Endothel Dependent and Independent Vasodilatation in Human Gingiva: Comparison of the Effect of NO and ACH

Conditions
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Vasodilatation, Oral Soft Tissue Conditions

Intervention / Treatment
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* Drug: Nitroglycerin
* Drug: Acetylcholine


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: general health minimum 5 mm keratinized gingiva at the upper front teeth good oral hygiene Exclusion Criteria: pregnancy and breast-feeding smoking medication insufficient marginal integrity periodontitis caries

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: nitroglycerin<br>NO donor (Nitropohl) vs pgysiological saline 1mg/ml nitorhlycerin | Drug: Nitroglycerin<br>* Vasodilatator solution is dropped on the labial surface on the lateral incisor, so it can penetrate through the sulcus.<br>|
| Active Comparator: acethylcholine<br>10 mg/ml acetylcholine vs physiological saline | Drug: Acetylcholine<br>* Vasodilatator solution is dropped on the labial surface on the lateral incisor, so it can penetrate through the sulcus.<br>* Other names: Ach;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood flow change after applying the test solutions | The change in blood flow will be measeured after applying the test solutions. | 20 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Comparison the change in blood flow between the two solutions | The blood flow values will be evaluated after each applied solutions. | 30 minutes | 
| Comparison the change in blood flow in genders | The maximal blood flow changes assessed in primary outcome will be split into two groups based on gender. The change will be compared between males and females. | 20 minutes | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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spreading vasodilatation, gingial microcirculation, LSCI