A Comparison of Clinical Outcomes of Warmed Embryo Vitrified at 2PN Stage and Fresh Embryo Transfers

Study Overview
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Brief Summary
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The aim of this study was to compare the clinical outcomes between fresh embryo and frozen embryo transfer in in vitro fertilization (IVF). All frozen embryo was vitrified at pronuclear stage (2PN) and cultured to cleavage embryos for transfer. This is a retrospective study. All IVF cycles were included in this study and further divided into fresh and frozen embryo transfer groups. The collection data including: stimulation protocols, medicines, blood test results, pregnancy test and all clinical data. The primary outcome is pregnancy rate and secondary outcome is implantation rate.

Detailed Description
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Study design Study progress: Stage 1: collection of all data from frozen embryo transfer and fresh embryo transfer groups. Stage 2: selection data and check the delivery outcomes Stage 3: analysis data and presentation. Including criteria: all patients who underwent IVF. Excluding criteria: No. Statistics analysis: chi-test, t-test Assessment point: Primary endpoint: pregnancy rate Secondary endpoint: implantation rate Withdraw criteria: not application. Treatments: not application. Collection number: 500 patients. Scheduled trial period: 2017.05.01 2018.04.30 Expected research results: The implantation rate in frozen embryo transfer group which vitrified at 2PN stage is higher than fresh embryo transfer. The pregnancy rate in frozen embryo transfer group which vitrified at 2PN stage is higher than fresh embryo transfer. This study is only data analysis, no additional collection of samples. This study does not involve vulnerable groups.

Official Title
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Chung Shan Medical University Hospital

Conditions
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Infertility, Female, Reproductive Disorder

Intervention / Treatment
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* Diagnostic Test: progesterone
* Diagnostic Test: day 3 embryo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: all patients with in vitro fertilization protocols Exclusion Criteria: none

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 55 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| fresh embryo transfer<br>patients with fresh day 3 embryo transfer after oocyte pick up, the hormone including FSH, LH, E2 and progesterone was test before embryo transfer for analysis. The clinical outcomes including implantation and pregnancy were checked and recorded. | Diagnostic Test: progesterone<br>* according progesterone level to decide fresh or frozen embryo transfer<br>Diagnostic Test: day 3 embryo<br>* according day 3 embryo to decide fresh or frozen embryo transfer<br>|
| frozen embryo transfer<br>freeze-all embryo was performed after oocyte fertilization, the hormone including FSH, LH, E2 and progesterone was test before oocyte pick up and embryo transfer for analysis. vitrification of pronuclear stage embryo (zygote) and frozen embryo transfer | Diagnostic Test: progesterone<br>* according progesterone level to decide fresh or frozen embryo transfer<br>Diagnostic Test: day 3 embryo<br>* according day 3 embryo to decide fresh or frozen embryo transfer<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| pregnancy rate | HCG test | HCG test at day 15 after embryo transfer or day 18 after oocyte pick up |