Microflora and Metabolomics of Crohn's Disease Patients

Study Overview
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Brief Summary
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The purpose of this study is to explore the microflora characteristics of different ecological loci and multiple metabolic pathways in Crohn's disease patients with different disease course states.

Detailed Description
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This study aims to investigate the characteristics of microflora in the oral and tongue base, small intestinal stomy and lower respiratory tract, as well as the characteristics of multiple metabolic pathways such as bile acid metabolism and amino acid metabolism in Crohn's disease patients with different course states.

Official Title
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A Study Focused on the Microflora and Metabolomics of Crohn's Disease Patients

Conditions
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Crohn Disease

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: individuals who were clear and signed the informed consent. Male and female. Exclusion Criteria: Any one who can not provide accurate information. Any one who denies the study.
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Crohn's disease group<br>Patients diagnosed with Crohn's disease after comprehensive evaluation of clinical symptoms, endoscopic features, radiological manifestations and histological features were recruited, and patients with CDAI score ≥ 150 were included in this group. | |
| Crohn's disease remission group<br>Patients diagnosed with Crohn's disease after comprehensive evaluation of clinical symptoms, endoscopic features, radiological features and histological features were recruited, and patients with CDAI score < 150 were included in this group. | |
| Healthy control group<br>Normal healthy people were randomly recruited as the control group | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oral microbiota structure and function difference between active stage of Crohn's disease, remission of Crohn's disease and healthy controls | The results were based on the 16S rRNA sequencing | 3 months | 
| Small intestinal microbiota structure and function difference between active stage of Crohn's disease, remission of Crohn's disease and healthy controls | The results were based on the 16S rRNA sequencing | 3 months | 
| Sputum microbiota structure and function difference between active stage of Crohn's disease, remission of Crohn's disease and healthy controls | The results were based on the 16S rRNA sequencing | 3 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolic difference of blood samples between active stage of Crohn's disease, remission of Crohn's disease and healthy controls | The metabolites of the blood samples by LC-MS will be detected | 3 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Crohn's disease, microbiota, metabonomics