Drinking Effect of Electrolyzed Alkaline Reduced Water on Oxidative Stress and Fatigue After Intense Exercise

Study Overview
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Brief Summary
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The goal of this clinical trial is to test the drinking effect of electolyzed alkaline reduced water (ARW) generated from alkaline ionizer (CGM MWPI-2101) on oxidative stress and fatigue after high-intensity exercise in healthy people. The main question[s] it aims to answer are: [question 1] Can pH 9.5 EARW reduce oxidative stress compared to purified water (PW) [question 2] Can pH 9.5 EARW decrease fatigue markers in blood Participants will drink water from the experimental device after high-intensity exercise. Blood sample will be collected before exercise, after highly intensive exercise, and after then 15 min. of drinking water. Researchers will compare EARW group and PW group to see the anti-oxidative and anti-fatigue effects.

Detailed Description
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This study was designed as a randomized, controlled, crossover, double-blind clinical trial with a single intervention of ARW intake (pH 9.5, 10 mL/kg body weight) after highly intense exercise. The participants were divided into two groups, wherein they consumed either puriﬁed water (PW group) or EARW (EARW group). Blood samples were collected before exercise, immediately after exercise, and 15 min after drinking water. For the anti-oxidative effect, ROS, NO, GPx, and MDA levels were measured. For the anti-fatigue effect, latate, lactate dehydrogenase, phosphate and calcium were measured.

Official Title
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Effects of Alkaline-reduced Water Generated From Electrolyzed Device (CGM MWPI-2101) on High-intensity Exercise-induced Oxidative Stress and Fatigue in Young Male Healthy Adults: Controlled, Randomized, Double-blind Clinical Trial

Conditions
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Oxidative Stress

Intervention / Treatment
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* Device: Electrolyzed alkaline reduced water generator


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy male participants aged 19-25 years physically active with a normal body mass index (BMI) had not smoked for 3 months or consumed alcohol for more than a week prior to the start of the study. Exclusion Criteria: any kind of visible or known disease hypertension, musculoskeletal injuries in the previous three months, breathing difﬁculties, metabolic diseases, autoimmune disease, rash, urticaria.

Ages Eligible for Study
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Minimum Age: 19 Years
Maximum Age: 25 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Screening
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: The participants were randomly divided into two groups (n = 12) according to the sequence of random numbers obtained through an online number generator. After the first clinical trial, A wash-out period of one week was applied to prevent carry-over before crossing over, and the second clinical trial was performed.
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: EARW group<br>This group drink electrolyzed alkaline reduced water (EARW, pH 9.5) 10 mL/kg body weight in 10 min after exercise. | Device: Electrolyzed alkaline reduced water generator<br>* Participants drink water (10 mL/kg body weight) generated from the device after intensive excercise.<br>* Other names: Electrolyzed alkaline ionizer (CGM MWPI-2101;|
| Sham Comparator: PW group<br>PW is purified water generated from sham device, and PW group drink 10 mL/kg body weight in 10 min after exercise. | Device: Electrolyzed alkaline reduced water generator<br>* Participants drink water (10 mL/kg body weight) generated from the device after intensive excercise.<br>* Other names: Electrolyzed alkaline ionizer (CGM MWPI-2101;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| EARW consumption is effective in reducing oxidative stress | Reactive oxygen species, nitric oxide, glutathione peroxidase, malondialdehyde | 15 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The effects of EARW consumption on exercise-induced fatigue markers in healthy young adults | Lactate levels, phosphate levels, cytokines | 15 minutes |