Elevated FFA and Skeletal Muscle Lipid Content

Study Overview
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Brief Summary
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There is increasing evidence that skeletal muscle lipid content (IntraMyoCellular Lipid, IMCL) markedly increases the risk of metabolic complications, including insulin resistance and cardiovascular events. The investigator hypothesizes that skeletal muscle is passively taking up FFAs when the availability is high, thereby leading to an increased storage. To test this hypothesis, the investigator wants to manipulate FFA levels, by means of exercise, and monitor intramuscular lipid content. Therefore the objective is to examine the effect of an exercise-induced elevation of FFA on skeletal muscle lipid content in healthy lean men. To this end, skeletal muscle lipid content will be investigated at baseline and after an exercise protocol and again after a four-hour recovery period from exercise, once in a condition with high FFA concentration, once with low FFA concentration. To achieve high- versus low FFA concentrations, an exercise protocol was chosen and participants had to perform this protocol once with a glucose supplementation and once without. Skeletal muscle lipid content will be determined before, directly after exercise and 4 h post exercise (from muscle biopsies) with or without glucose supplementation.

Official Title
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Effects of Acute Elevation of Circulating Fatty Acids on Skeletal Muscle Lipid Accumulation and Metabolism in Healthy Lean Young Men

Conditions
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Diabetes, Exercise Physiology

Intervention / Treatment
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* Other: Exercise
* Other: Glucose
* Other: Fasted


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male sex Age 18-30 years Lean, BMI 18-25 kg/m2 Healthy Stable dietary habits No medication use Exclusion Criteria: Female sex Engagement in programmed exercise > 2 hours total per week Cardiac problems, such as angina pectoris, cardiac infarction and arrhythmias First degree relatives with type 2 diabetes mellitus Any medical condition requiring treatment and/or medication use Unstable body weight (weight gain or loss > 3 kg in the past three months) Participation in other biomedical study within 1 month prior to the screening visit Subjetcs, who do not want to be informed about unexpected medical findings, or do not wish that their treating physician is informed, cannot participate in the study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Exercise & Glucose<br>2 hour exercise while getting glucose-supplementation | Other: Exercise<br> <br> Other: Glucose<br> <br> |
| Experimental: Exercise & Fasted<br>2 hour exercise while staying fasted | Other: Exercise<br> <br> Other: Fasted<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in IntraMyoCellularLipid (IMCL)content | A muscle biopsy will be withdrawn before, after 2 hours of exercise and again 4 hours post-exercise for later analysis of IMCL. | Baseline, directly postexercise and 4 hours post-exercise | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in substrate metabolism | Substrate metabolism will be measured for 15 min every 30 min of exercise and 20 min every hour of recovery for later analysis of fat- and glucose oxidation. | Measured several time points during the test day | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Exercise, Ectopic fat accumulation