The Impact of Red Meat and Whole-grains Intake on the Colonic Mucosal Barrier

Study Overview
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Brief Summary
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This study evaluates the impact of red meat and whole-grain intake on the colonic mucosal barrier and the dietary impact of these groups on the induced low-grade inflammation

Detailed Description
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The mucus layer in the colon is a continuous barrier separating the epithelial cells from faecal enzymes, bacteria, toxic and other components. The colonic mucus layer in patients with Crohn's Disease and Ulcerative Colitis was previously demonstrated to be thinner and more permeable compared to healthy subjects. The colonic mucus is composed of glycoproteins held together trough unstable disulfide bridges. The digestion of red meat is associated with monosulfide production, which could bind to the disulfide molecules, make more stable trisulfide molecules, and thus destroy the mucus architecture. Moreover, different bacteria were previously linked to red meat intake and were associated with a degradation of the colonic mucus such as akkermansia muciniphila. From the other side, the fermentation of the undigested fibres, primarily in whole-grains, is associated with the production of short-chain fatty acids, which was related to a local anti-inflammatory effect. In this study, we hypothesise: High consumption of red meat is associated with a thinner colonic mucus layer; High consumption of whole-grain fibres is associated with a thicker colonic mucus layer; Mucin-2 gene expression is different between patients with high red meat consumption vs low red meat consumption; Mucin-2 gene expression is different between patients with high whole-grain consumption vs low whole-grain intake; The level of inflammatory markers in blood IL-1beta, IL-4, IL-6, IL-10, Hs-CRP, and TNF-alfa is higher in subjects with high red meat and low whole-grain intake.

Official Title
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The Impact of Red Meat and Whole-grains Intake on the Colonic Mucus' Thickness, Colonic MUC-2 Expression Rate and the Inflammatory Markers in the Blood.

Conditions
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Mucus Colitis, Inflammation, Colon, Dietary Exposure

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subjects referred to a colonoscopy in the Hospital of Southern Denmark Subjects able to read and understand Danish Mentally habile subjects Subjects who accept to be a part of the project Exclusion Criteria: History of active cancer A recent colonoscopy (less than 3 months ago ) Anamnesis of Inflammatory bowel diseases.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 99 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| BELIEVE<br>Consists of subjects referred to a colonoscopy between June 1, 2017, and December 1, 2019, in the hospital of Southern Denmark | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Colonic mucus thickness | We will evaluate the thickness of the inner mucus layer in 38 biopsies of subjects, at the upper and lower quartiles of red meat and whole-grain consumption | November 30,2019 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muc-2 gene expression | Using real time PCR, we will examine the association between the expression of mucin 2 gene in relation to red meat and whole grain fiber intake | December 10, 2019 | 
| Inflammatory markers in blood (IL-1beta, IL-4, IL-6, IL-10, Hs-CRP, and TNF-alfa) | These markers will be evaluated in relation to red meat and whole-grain intake | January 1-2020 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Colonic mucus, Mucosal barrier, Red meat, Whole grain, Dietary fibers, Inflammation, Muc-2