Anesthesia and Lymphocytes Apoptosis

Study Overview
=================
Brief Summary
-----------------
Most researches revealed that volatile anesthetic sevoflurane has a more marked cardioprotective effect against ischemia compared with intravenous agent propofol.However, propofol has been suggested to be more benefit for attenuation of surgery-induced immunosuppression.Thus, the aim of this study was to investigate under the specific OPCAB surgical stress, the influence of 3 established anesthetic techniques: sevoflurane, propofol and combine of sevoflurane and propofol maintained anesthesia on the apoptosis of circulating lymphocytes.

Official Title
-----------------
Different Effects of Sevoflurane, Propofol and Combine of Sevoflurane and Propofol Maintained Anesthesia on Peripheral Blood Lymphocytes During Off-pump Coronary Artery Bypass Graft Surgery

Conditions
-----------------
Anesthesia

Intervention / Treatment
-----------------
* Drug: Sevoflurane
* Drug: Propofol
* Drug: combine of sevoflurane and propofol


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: male adults aged 18-80 yr undergoing elective OPCAB surgery containing left anterior descending artery bypass Exclusion Criteria: a previous unusual response to any of the experimental anesthetics severe cardiac dysrhythmias or ejection fraction below 30% hemodynamic instability previous surgical coronary artery repair anemia, abnormal leukocytes or coagulopathy severe hypertension severe hepatic (albumin<30g,ascites), renal (serum creatinine greater than 2.0 mg/dl) or pulmonary (preoperative pulmonary function tests moderate-severe) dysfunctions concomitant surgical procedures and psychiatric disorders Insulin-dependent diabetics

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
-----------------
Male

Accepts Healthy Volunteers
-----------------
No

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Sevoflurane<br> | Drug: Sevoflurane<br>* anesthesia was maintained with sevoflurane (end-tidal concentration 1.0-1.5 minimum alveolar concentration)<br>|
| Experimental: Propofol<br> | Drug: Propofol<br>* anesthesia was maintained with propofol (2-4 μg/ml via target-controlled infusion)<br>|
| Experimental: Combine of sevoflurane and propofol<br> | Drug: combine of sevoflurane and propofol<br>* anesthesia was maintained with a combine of propofol (1 μg/ml via target-controlled infusion) and sevoflurane (end-tidal concentration 0.7-1.0 minimum alveolar concentration)<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Lymphocyte Count | Blood samples were obtained 24 h after the surgery for routine blood examination. This analysis was performed in the hospital laboratory using routine laboratory procedures. | 1 day after surgery | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Intensive Care Unit Staying Days |  | participants will stay in intensive care unit after surgery, an expected average of 2 days | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
troponin I, creatine kinase mb, hospital days, lymphocyte apoptosis, intensive care unit days