Gingival Crevicular IL-34 and CSF-1 Levels in Patients With Periodontitis and Uncontrolled Type 2 Diabetes

Study Overview
=================
Brief Summary
-----------------
Type 2 diabetes mellitus is a metabolic disorder. There is a bidirectional association between periodontal disease and DM. Currently there is no data relationship between CSF-1 and IL-34, periodontal disease and diabetes.The aim of this study to investigate GCF levels of IL-34 and CSF-1 in periodontitis subjects with diabetes.

Detailed Description
-----------------
Periodontally healthy (H), periodontitis, and periodontitis with diabetes patients, were recruited for this study. Inclusion criteria for H groups: Individuals with clinically healthy gingiva, no attachment and bone loss, and less than 10% bleeding on probing were included. Inclusion criteria for periodontitis groups: Systemically healthy individuals with interdental CAL ≥ 5 mm and PPD ≥ 6 mm were included. Inclusion criteria for periodontitis with diabetes groups: Type 2 diabetes patients with interdental CAL ≥ 5 mm, PPD ≥ 6 mm, and HbA1c ≥ 6.5% were included. The plaque index (PI), gingival index (GI), presence of bleeding on probing (BOP), PD, and clinical AL were used for clinical periodontal assessments. All assessments were done with a manual probe. GCF samples were obtained and analyzed using an enzyme-linked immunosorbent assay.

Official Title
-----------------
Usak University Faculty of Dentistry

Conditions
-----------------
Diabetes, Periodontitis

Intervention / Treatment
-----------------
* Diagnostic Test: Gingival crevicular fluid


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: have 20 teeth have diabetes . Exclusion Criteria: Individuals have systemic disease smokers; pregnant those using drugs;

Ages Eligible for Study
-----------------
Minimum Age: 20 Years
Maximum Age: 65 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy control groups<br>individuals with clinically healthy gingiva BOP score less than 10% and PD≤3mm | Diagnostic Test: Gingival crevicular fluid<br>* GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.<br>|
| Periodontitis group<br>interdental AL ≥5 mm | Diagnostic Test: Gingival crevicular fluid<br>* GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.<br>|
| Periodontitis with diabetes group<br>HbA1c ≥6.5% | Diagnostic Test: Gingival crevicular fluid<br>* GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| It was aimed to compare IL-34 levels by taking gingival crevicular fluid in systemic healthy, periodontitis and diabetic patients with periodontitis groups. | GCF was taken interproximal region. | GCF samples taken within the 1. week after the clinical examination of the patient | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| It was aimed to compare CSF-1 levels by taking gingival crevicular fluid in systemic healthy, periodontitis and diabetic patients with periodontitis groups. | GCF was taken interproximal region. | GCF samples taken within the 1. week after the clinical examination of the patient | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
Diabetes,, periodontitis, cytokines