Evidence of Spreading Vasoconstriction in Human Gingiva

Study Overview
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Brief Summary
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The aim of this study is to observe the mechanism of spreading vasoconstriction in human healthy gingiva. Epinephrine solution is applied on the attached gingiva in group A and on the surface of the tooth next to the ginvial sulcus in group B. The different placement of the solution causes different effect in the microcirculation.

Detailed Description
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Spatial regulation of gingival microcirculation has not been revealed yet. Although this could be an important mechanism to protect gingiva as it is exposed to mechanical, chemical, thermal etc. irritation during whole life. It is especially important during flap surgery. Laser Speckle Contrast Imaging (LSCI) is novel non-invasive method with high spatio-temporal resolution, therefore it allows us to study remote effect of local vasoconstriction such as spreading vasoconstriction in human gingiva. The epinephrine is a daily used vasoconstrictor material in dentistry and it will be used to trigger remote effect on gingiva. Epinephrine in 1 mg/ml concentration. Solution will be dropped on the gingiva at tooth 12 in a fabricated leakage proof well attached to 2mm above the marginal gingiva in group A. The solution will be dropped on the surface of tooth 12 next to the sulcus into the well in case of group B. The changes of the blood flow will be monitor at the test side with surrounding gingival area by LSCI. The control side in each case will be - appropriately to the test side - at tooth 21 with phisiologocal saline.

Official Title
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Evidence of Spreading Vasoconstriction in Human Gingiva

Conditions
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Vasoconstriction, Oral Soft Tissue Conditions, Dental and Gingival Conditions

Intervention / Treatment
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* Drug: Epinephrine


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: at least 5 mm keratinized gingiva at the upper front teeth general health Exclusion Criteria: pregnancy, breast-feeding any medication smoking, gingivitis, caries and coronal restoration with insufficient marginal integrity

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Epinephrine effect throught keratinized ginigva<br>1 mg/ml epniephrine vs physiologocal saline | Drug: Epinephrine<br>* Vasoconstrictor solution is applied on the surface of the attached gingiva and on the gingival sulcus.<br>* Other names: retrograde vasoconstriction;|
| Experimental: Epinephrine effect throught gingival sulcular epithelium<br>1 mg/ml epniephrine vs physiologocal saline | Drug: Epinephrine<br>* Vasoconstrictor solution is applied on the surface of the attached gingiva and on the gingival sulcus.<br>* Other names: retrograde vasoconstriction;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood flow change after application of epinephrine | The change in blood flow will be measured after locally applied epinephrine at the site fo application and at the remote sites | 30 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Comparsion of the change in blood flow between genders | The maximal blood flow changed assessed in primary outcome will be split into two groups based on gender. The change will be compared between males and females. | 30 minutes | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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spreading vasoconstriction, gingival microcirculation