The Effects of Exercise of Secreted Factors From Muscle and Adipose Tissue

Study Overview
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Brief Summary
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The purpose of this study is to find out whether exercise leads to changes in the blood that are produced by exercised muscles and if these changes produce new hormones that affect the body's regulation of sugar and body weight.

Detailed Description
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Recent work in the laboratory at the Joslin Diabetes Center and elsewhere has shown that when small animals exercise they improve their muscles and improve their blood sugar levels. Also, these animals show changes in their abdominal fat tissue, compared to animals that did not exercise. Our data suggest that factors released from exercised muscle and fat tissue from exercised mice also has a positive effect on other tissues in the same animal. Therefore, there could be new factors or hormones, which come from the trained animals' muscles or fat tissue and which exert a positive effect on the animals' blood sugar levels. Large studies with human volunteers have shown that exercise can improve blood sugar uptake into skeletal muscle and lower blood sugar levels, thereby preventing type 2 diabetes. The purpose if this study is to evaluate if a single exercise bout leads to changes in circulating factors in the blood. We plan to detect if there are hormones being produced in the body that have a beneficial effect elsewhere in the body.

Official Title
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Investigation of Exercise-Induced Myokines and Adipokines

Conditions
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Healthy

Intervention / Treatment
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* Other: Exercise bout


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Age between 18-35. Body mass index (BMI) must be ≥20 and ≤26 kg/m2. HbA1c values ≤5.7% Exclusion Criteria: - Age <18 and >35; HbA1c ≥ 5.7%; heart or lung disease; acute systemic infection accompanied by fever, body aches, or swollen lymph glands; BMI ≥ 26 kg/m2; current dieting or weight loss efforts; current pregnancy or breastfeeding; known history of HIV/AIDS; cancer; biochemical evidence of renal or hepatic dysfunction; renal or liver disease; demyelinating diseases such as multiple sclerosis or amyotrophic lateral sclerosis; recent blood donation; clinical history of stroke; hypertension (systolic > 140 mmHg or diastolic > 90 mmHg); type 1 or 2 diabetes; history of keloid formation inability to exercise at 50% of predicted heart rate (HR) reserve at baseline. Participants taking beta-blockers Participants who screen positive for The American Heart Association's contraindications to exercise testing

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Exercise intervention<br>Subjects will undergo an acute bout of exercise for 45 mins at 75% of peak aerobic capacity | Other: Exercise bout<br>* Participants will exercise for 45min at 75% of VO2peak<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in myokines/adipokine concentration |  | baseline, and 15, 45 and 1h:45 mins after exercise session. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Exercise