New Biomarkers in the Intracystic Liquid of Inflammatory and Non-inflammatory Pancreatic Cysts

Study Overview
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Brief Summary
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Due to the advances made in the field of the imaging techniques and their common use, pancreatic cysts are more and more incidentally found. Pancreatic pseudocysts can be misdiagnosed as cystic neoplasms, in particular when there is no clinical history of acute or chronic pancreatitis. Pancreatic cystic neoplasms on the other hand are rare, but are difficult to diagnose accurately. Neutrophil gelatinase-associated lipocalin (NGAL) , interleukin 1 Beta (IL1beta) and High Mobility Group AT-Hook 2 (HmgA2) are molecules implicated in the process of inflammation and tumour development. The diagnostic value of their concentration in pancreatic cysts is not established yet. Study aim to asses the significance of NGAL,IL1Beta and HMGA2 concentration in cystic fluid obtained by endoscopic ultrasound (EUS) with EUS-guided fine-needle aspiration (EUS-FNA) and serum level for discriminating between inflammatory and non-inflammatory pancreatic cyst.

Official Title
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New Biomarkers in the Intracystic Liquid of Cystic Pancreatic Lesions

Conditions
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Pancreatic Cyst

Intervention / Treatment
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* Diagnostic Test: Endoscopic ultrasound fine needle aspiration (EUS-FNA)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: patients aged 18-90 years with an unclear specific diagnosis of pancreatic cysts pancreatic cyst >15mm on CT or MRI patients with pseudocysts with indication for drainage Exclusion Criteria: refusal to participate platelet level < 50000/mm3 and International normalized ratio (INR >1.5) duodenal stenosis severe chronic pancreatitis history of pancreatic cancer or major upper abdominal surgery congestive heart failure the liquid sample < 1ml;

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 90 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Diagnostic Test: Endoscopic ultrasound fine needle aspiration (EUS-FNA)|Patients with pancreatic cysts who underwent endoscopic ultrasound and endoscopic fine needle aspiration for diagnostic and therapeutic purpose. A sample of the cyst fluid and frozen serum was collected from the patients and stored at minus 80 degrees.|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Role of the cyst fluid concentrations of the specific biomarkers for differentiation the pancreatic cystic lesions | Measurement the cyst fluid concentration of NGAL, IL1beta, HMGA2, expressed in nanograms/dl | through study completion, an average of 1 year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The Correlation between cyst fluid concentration and serum level of the specific biomarkers | coefficient of correlation between the two values | through study completion, an average of 1 year | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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pancreatic cyst, EUS-FNA, intracystic fluid