Serum Levels of Apelin, Chemerin and Adiponectin Adipokines in Irritable Bowel Syndrome and Healthy Subjects

Study Overview
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Brief Summary
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In this observational case-control study investigators aimed to assess the serum levels of adipokines such as Apelin, Chemerin and adiponectin in patients with irritable bowel syndrome (IBS), and compare it with healthy controls. Furthermore, investigators evaluated the possible correlation of these adipokines with IBS-QoL, IBSSS, GI symptoms and other clinical and psychological disorders.

Official Title
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Evaluation of the Serum Levels of Apelin, Chemerin and Adiponectin Adipokines in Patients With Irritable Bowel Syndrome and Healthy Subjects

Conditions
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Irritable Bowel Syndrome

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Men and women between the ages of 18, and 80 years. IBS patients diagnosed according to the Rome III Diagnostic Criteria for Functional GI Disorders. Exclusion Criteria: Any evidence of abdominal surgery or radiation celiac disease, or other primary GI illnesses GI infection obscuring IBS symptoms, using total parenteral nutrition therapy in the last 6 months pregnancy and lactation alcohol consumption concurrent chronic diseases such as diabetes, renal failure diagnosed and/or treated malignancy in the past 5 years anti-inflammatory drugs usage intake of vitamin D, omega-3, vitamin E, and calcium supplements being on a special diet or medication regimen during the last 6 months

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Case<br>patients with irritable bowel syndrome | |
| Control<br>Healthy subjects without any gastrointestinal disorders, chronic diseases and malignancy. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serum levels of Apelin, Chemerin and adiponectin |  | in recruitment | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| IBS-Quality of life (IBS-QoL) | The Quality of life was assessed at the baseline and at the end of the study using a self-report QoL measure specific to IBS (IBS-QoL) with 34 items | in recruitment | 
| IBS severity score system (IBSSS) | Irritable bowel syndrome severity score system is validated for use in IBS patients, which included five clinically relevant items during a 10-day period: (i) severity of abdominal pain, (ii) frequency of abdominal pain, (iii) severity of abdominal distention or tightness, (iv) dissatisfaction with bowel habits, and (v) interference of IBS with life in general. Each item was scored on a scale from 0 to 100, and the sum of these five items was the score of IBS severity (range 0-500). Scores of 75-175, 175-300, and >300 indicated mild, moderate, and severe cases, respectively. | in recruitment | 
| Gastrointestinal symptoms |  | in recruitment | 
| Somatic Symptom |  | in recruitment | 
| Personality | Big Five Inventory Questionnaire | in recruitment | 
| Perceived Stress Scale | The Perceived Stress Scale (PSS) is the most widely used psychological instrument for measuring the perception of stress. It is a measure of the degree to which situations in one's life are appraised as stressful.Higher PSS scores were associated with a greater vulnerability to stressful life-event-elicited depressive symptoms. PSS scores are obtained by reversing responses (e.g., 0 = 4, 1 = 3, 2 = 2, 3 = 1 & 4 = 0) to the four positively stated items (items 4, 5, 7, & 8) and then summing across all scale items. A short 4 item scale can be made from questions 2, 4, 5 and 10 of the PSS 10 item scale. | in recruitment |