Proteins From Duckweed Trial

Study Overview
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Brief Summary
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This study aims to study the digestibility of isolated duckweed protein. The digestibility of the isolated duckweed protein will be compared to an isolated benchmark protein whey. Objective: The primary objective is to investigate the digestibility of isolated duckweed protein and the isolated benchmark protein whey. Study design: The study is a cross-over, double blind, controlled trial in which study participants will visit the research facility on two occasions under fasting conditions. Subjects will receive two protein sources in randomized order with a washout period of one week. Blood will be collected via a catheter before and up-to 3 hours after protein consumption. Study population: We aim to include 12 healthy volunteers aged 18-50 years. Intervention: Study participants will receive 20 grams of isolated duckweed protein and the isolated benchmark protein whey. Main study parameters/endpoints: The main study parameter is blood amino acids measured before and after consumption of 20 g protein.

Official Title
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Proteins From Duckweed Trial

Conditions
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Degree of Digestilbility

Intervention / Treatment
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* Dietary Supplement: Duckweed protein
* Dietary Supplement: Whey protein


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Body mass index (BMI) between 18 and 25 kg/m2 Having veins suitable for blood sampling via a catheter (judged by study nurse/ medical doctor) Exclusion Criteria: Any metabolic, gastrointestinal, inflammatory or chronic disease (such as diabetes, anaemia, hepatitis, cardiovascular disease) History of gastro-intestinal surgery or having (serious) gastro-intestinal complaints History of liver dysfunction (cirrhosis, hepatitis) or liver surgery Kidney dysfunction (self-reported) Use of medication that may influence the study results, such as gastric acid inhibitors, laxatives, stomach protectors and drugs that can affect intestinal motility Anaemia (Hb values <7.5 for women and <8.5 for men) Reported slimming, medically prescribed, vegan/vegetarian or other extreme diets or the use of protein supplements Not willing to give up blood donation during the study Current smokers Alcohol intake ≥4 glasses of alcoholic beverages per day Pregnant, lactating or wishing to become pregnant in the period of the study (self-reported) Abuse of illicit drugs Food allergies for products that we use in the study Participation in another clinical trial at the same time Being an employee of the department Consumer Science & Health group of Wageningen Food & Biobased Research

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Duckweed protein<br>20 gram of isolated protein | Dietary Supplement: Duckweed protein<br>* 20 grams isolated protein<br>|
| Experimental: Whey protein<br>20 gram of isolated protein | Dietary Supplement: Whey protein<br>* 20 grams isolated protein<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in blood free amino acids (e.g. Degree of digestibility) | postprandial test, determine free amino acids in blood samples collected before and after a postprandial challenge test | 20 gram protein will be consumed and blood samples will be taken before and up to 3 hours after protein consumption | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in plasma insulin | before and after a postprandial challenge test | 20 gram protein will be consumed and blood samples will be taken before and up to 3 hours after protein consumption | 
| change in plasma glucose | before and after a postprandial challenge test | 20 gram protein will be consumed and blood samples will be taken before and up to 3 hours after protein consumption | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Duckweed protein, protein, degree of digestibility, whey protein