Genetic Variants and Iron Absorption

Study Overview
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Brief Summary
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The two specific aims of this study are: 1) to assess the impact of a genetic variant on iron status; 2) to assess the impact of a genetic variant on non-heme absorption among Asian women. The investigators hypothesize that the genetic variant could enhance iron status and iron absorption in Asian women.

Detailed Description
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To determine the HFE genotype of female Asian study volunteers, we collected venous blood samples and extracted DNA from these blood samples. HFE genotype was determined as CC, CT, and TT and used to assess possible differences in iron status as a function of genotype. To measure the impact of genotype on non-heme Fe absorption, a sub-group of women with the CC (n=10) or TT (n=11) genotype were invited to return for an Fe absorption study. Each volunteer consumed stable 57Fe (as ferrous sulfate). Two weeks after ingesting this tracer, a blood sample was collected from each woman and the amount of non-heme (57Fe) iron incorporated into red blood cells was measured with magnetic sector thermal ionization mass spectrometry. Possible associations between iron status and iron absorption as a function of genotypes were explored. Data from this study will provide information that aims to improve human health by better understanding the iron requirements of individuals with different genetic background.

Official Title
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Metabolic Adaptation to Plant-based Diets in Asian Populations

Conditions
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Iron Absorption

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: non-pregnant and between the ages of 18 35 y East Asian descent with both maternal and paternal grandparents from East Asia, including China, Japan, South Korea, Vietnam, Thailand, Malaysia and Singapore Exclusion Criteria: taking or planing to take any vitamin or mineral supplements during the study period pre-existing medical problems that might impact inflammation or Fe status, including malabsorption, blood disorders, ulcers, joint diseases and asthma taking any prescribed medications known to affect iron homeostasis

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Genotype at SNP rs9366637 | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining genotype. | 2 weeks after the participant's visit to the laboratory | 
| Hemoglobin | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of hemoglobin. | Up to 2 days after the participant's visit to the laboratory | 
| Serum Ferritin | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum ferritin. | Up to 6 months after the participant's visit to the laboratory | 
| Serum transferrin receptor | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum transferrin receptor. | Up to 6 months after the participant's visit to the laboratory | 
| Non-heme Iron absorption in women with 2 HFE genotypes | Women donated a blood sample for genotyping. Women with particular HFE genotypes were invited to return for an absorption study. Two weeks after women consume the iron tracer, a blood sample was obtained to measure the amount of iron absorbed. | one month for screening with genotype then 2 weeks for iron absorption study | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Folate | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of folate. | Up to 6 months after the participant's visit to the laboratory | 
| Vitamin B-12 | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of vitamin B-12. | Up to 6 months after the participant's visit to the laboratory | 
| C-reactive protein | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of C-reactive protein. | Up to 6 months after the participant's visit to the laboratory | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Non-heme iron absorption; HFE