Mitochondrial Bioenergetics and Role in Cellular Damage in Ischemic Myocardium

Study Overview
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Brief Summary
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Cardiac ischemia is a common pathological condition, known to elicit multiple pathological processes at the cellular level. One of the most affected is thought to be cellular metabolism, key for the adequate cardiac function. The aim is to study mitochondrial bioenergetic function, interaction with other cellular systems and influence of several co-morbidities in myocardium of the affected patients.

Detailed Description
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Coronary artery disease, one of the most common pathologies in the developed world, causes hypoperfusion of myocardial tissue, usually evident by the presence of anginal pain. This myocardial ischemia elicits alterations in normal cardiomyocyte physiology, which gradually deteriorate cellular function, affecting the performance of the entire organ. The condition is frequently further complicated (and aggravated) by the presence of various co-morbidities, such as diabetes mellitus. The primary aim of this study is to investigate the cardiomyocyte bioenergetics and the consequences of potentially reduced mitochondrial metabolic function in ischemic heart, and evaluate the potential contribution of other conditions, primarily ones affecting metabolic homeostasis.

Official Title
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Pathophysiological Mechanisms Elicited by Ischemia and Metabolic Co-morbidities in Myocardium of Patients With Coronary Artery Disease

Conditions
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Coronary Artery Disease, Diabetes Mellitus, Mitochondrial Metabolism Disorders

Intervention / Treatment
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* Other: Standard therapy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Coronary artery disease Indication for coronary artery bypass grafting surgery Exclusion Criteria: Emergency patients Patients with LV ejection fraction (LVEF) below 30% Patients with severe renal, hepatic or pulmonary disease

Ages Eligible for Study
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Minimum Age: 50 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: Standard therapy|Patients with therapy prescribed independent of the study|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Mitochondrial oxygen consumption (rate of oxygen consumption per milligram of left ventricular tissue) | Mitochondrial respiration in nmol O2/minute/mg of tissue weight will be measured as an indicator of mitochondrial ATP production capacity, using different metabolic substrates | 2016-2020 | 
| Myocardial production of reactive oxygen species | Myocardial production of reactive oxygen species will be measured using electron paramagnetic resonance and fluorometry | 2016-2020 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Anti-cardiolipin antibodies in the blood of patients with symptoms of cardiac ischemia | Anticardiolipin antibodies assessed in venous blood of patients with angina pectoris undergoing coronary angiography | 2016-2020 |