Chronic Periodontitis and Polycystic Ovary Syndrome

Study Overview
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Brief Summary
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Background: The aim of this study was to investigate the levels of malondialdehyde (MDA), total antioxidant status (TAS), 8-hydroxy-2´- deoxyguanosine (8-OHdG) in blood serum and saliva samples in female patients with chronic periodontitis (CP) and to compared healthy individuals. Materials and Methods: A total of 88 women were into four groups each which were consisted of twenty-two subjects. Groups were designed as periodontally and systemically healthy women (PCOS-CP-); periodontally healthy women with polycystic ovary syndrome (PCOS) (PCOS+CP-), systemically healthy women with CP (PCOS-,CP+) and women with PCOS and CP (PCOS+CP+). Serum and salivary samples were obtained, clinical periodontal parameters were recorded. MDA, TAS, 8-OHdG levels were measured as biochemically.

Detailed Description
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RESULTS: Salivary 8-OHdG levels in the PCOSCP and CP groups were statistically higher than those in both the PCOSPH and the PH groups (P < 0.05). There was no statistical difference between the PCOSCP, CP, and PCOSPH groups with regard to salivary MDA and TAS levels (P > 0.05). Highest serum 8-OHdG and MDA levels and lowest serum TAS levels were seen in the PCOSCP group (P < 0.05). Serum 8-OHdG and MDA levels in the PCOSPH group were higher than those in both systemically healthy groups (PH and CP) (P < 0.05). Salivary TAS levels were highest (P < 0.05) in the PH group. There was no statistical difference between the CP and PCOSPH groups, but serum TAS levels were lower than those in the PH group (P < 0.05). CONCLUSIONS: CP, which led to an increase in serum and salivary 8-OHdG and MDA levels and a decrease in serum TAS levels in patients with PCOS, contributed to increased OS. This effect was more prominent in serum levels than in salivary levels.

Official Title
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Evaluation Of Lipid Peroxidation, Oxidative DNA Damage And Total Antioxidant Status In Patients With Periodontitis And Polycystic Ovary Syndrome: A Cross Sectional Study

Conditions
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Polycystic Ovary Syndrome, Chronic Periodontitis

Intervention / Treatment
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* Other: no intervention provided


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Newly diagnosed non-obese PCOS patients Never smokers Had no history of systemic disease BMI<25 kg/m2 Participants had ≥20 teeth present. Exclusion Criteria: Pregnancy Lactation Hemoglobin A1c (HbA1c) ≥ 6.5% 2-h oral glucose tolerance test (OGTT-2h) ≥200 Cushing syndrome, non-classic congenital adrenal hyperplasia, hyperprolactinemia, thyroid dysfunction, and androgen-secreting tumors Any drug use within the past 6 months Periodontal treatment within the past 6 months

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| (PCOS-CP-)<br>systemically and periodontally healthy participants | Other: no intervention provided<br> <br> |
| (PCOS-CP+)<br>systemically healthy participants with CP | Other: no intervention provided<br> <br> |
| (PCOS+CP-)<br>PCOS participants with periodontally healthy | Other: no intervention provided<br> <br> |
| (PCOS+CP+)<br>PCOS participants with CP | Other: no intervention provided<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 8-OHdG levels (nanogram per mililiter(ng/mL)) | by ELISA test | appoximately 2 years | 
| MDA levels (micromole per liter (µmol/L)) | by ELISA test | appoximately 2 years | 
| TAS levels (millimolar per liter (mmol /L)) | by ELISA test | appoximately 2 years | 
| Plaque Index (PI) | Silnes-Löe Periodondal İndex | appoximately 2 years | 
| Gingival Index (GI) | Löe-Silness Periodontal Index | appoximately 2 years | 
| Bleeding on probing (BOP) | Gİngival Bleeding Index | appoximately 2 years | 
| Clinical attachment level (CAL) | by measuring the distance between the cementoenamel junction and the periodontal pocket base | Patients were seen one time | 
| Probing pocket depth (PD)) | distance between free gingival margin and periodontal pocket base by usin periodontal probe | appoximately 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| number of individuals with chronic periodontitis | individuals in the group | appoximately 2 years | 
| numberer of individuals with polycystic ovary syndrome | individuals in the group | appoximately 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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8- hydroxy -2´- deoxyguanosine, Malondialdehyde, Total antioxidant status, Oxidative stres