Evaluation the Association of Microbiome Between Respiratory Tract Samples and Stool Samples in Pneumonic Patients Accompanied by Respiratory Failure

Study Overview
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Brief Summary
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A prospective observational study. Enrolled participants admitted to ICU due to pneumonia and respiratory failure need mechanical ventilator support. Investigators collected the residual specimens, such as sputum from endotrachea aspiration, bronchoalveolar lavage fluid in those participants as the usual care in the ICU. Those residual samples were sent to extract RNA and sequence by using high-throughput sequencing (next-generation sequencing) method. Investigators will compared the microbiome feature between lower respiratory tract and stool specimens in those participants diagnosed as pneumonia with respiratory failure.

Official Title
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Department of Critical Care Medicine, Taichung Veterans General Hospital

Conditions
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Microbiota, Pneumonia, Bacterial, Respiratory Failure, Lower Resp Tract Infection

Intervention / Treatment
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* Diagnostic Test: 16s RNA sequence of sputum, bronchoalveolar lavage fluid (options), stool


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adults aged than 40 years old Diagnosed as pneumonia Respiratory failure with mechanical ventilator support Exclusion Criteria: Less than 40 years old Has been treated with antibiotics more than 7 days within two weeks before enrolling into study Suspected or active pulmonary tuberculosis infection

Ages Eligible for Study
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Minimum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Pneumonia patient with respiratory failure<br>Sputum and stool sampling day 1, 3 and 7 after enrolling into study | Diagnostic Test: 16s RNA sequence of sputum, bronchoalveolar lavage fluid (options), stool<br>* We will check the microbiome in lower respiratory samples (sputum and bronchoalveolar lavage fluid) and stool by using 16S RNA sequencing<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The dynamics of the airway bacterial microbiome in the lower respiratory tract specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation) | Uses high-throughput sequencing (next-generation sequencing) to sequence the 16S rRNA sequence of bacteria in residual samples | Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The dynamics of the gut bacterial microbiome in the stool specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation) | uses high-throughput sequencing (next-generation sequencing) to sequence the 16S | Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7 | 
| The association of the microbiome between lower respiratory tracts and stool specimens in pneumonic patient with respiratory failure | Uses high-throughput sequencing (next-generation sequencing) to sequence the 16S | Compared the association of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Microbiota, Respiratory Failure, Pneumonia, Lower Respiratory Tract Infection