Effect of Basis™ and High Intensity Interval Training (HIIT) on Muscle Metabolism and Exercise Performance

Study Overview
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Brief Summary
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This is a single-center, randomized, placebo-controlled pilot study to evaluate the effects of high intensity interval training (HIIT) and daily administration of BasisTM for 3 weeks, in 18-35 year old males. The study will consist of a screening visit, baseline visit, followed by treatment and HIIT phase (3 weeks) and a post-supplementation, follow-up visit (this will occur 48 h (or more) after the final HIIT session). Following the screening phase, subjects who meet entry criteria will be randomly assigned to 1 of 2 treatment groups: recommended dose of BasisTM (250 mg Nicotinamide Riboside and 50 mg Pterostilbene) per day or matching-placebo in a 1:1 ratio.

Official Title
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The Effect of Basis™ and High Intensity Interval Training (HIIT) on Muscle Metabolism and Exercise Performance in Healthy 18 - 35 Year Olds

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: NRPT
* Dietary Supplement: Placebo oral capsule


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 to 35 year old males Body mass index between 18.5-30 kg.m-2 Ability to give informed consent Recreationally active (defined in the present study as exercising once a week or more). Exclusion Criteria: Those regularly conducting HIIT or highly trained in endurance events (e.g. triathlete) Currently taking supplements known to affect metabolism (e.g. ibuprofen, stimulants, vitamins, NR) Taking prescribed medication Consuming a vegan diet Consuming a high fat, low carbohydrate diet Habitual consumption of protein and/or dairy supplements (e.g. whey protein) Smokers

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: 1X dose of NRPT<br> | Dietary Supplement: NRPT<br>* Subjects randomly assigned to the active group will receive Nicotinamide Riboside and Pterostilbene (each capsule contains 125 mg NR and 25 mg PT, total daily dose is 2 capsules) for 3 weeks<br>* Other names: BasisTM;|
| Placebo Comparator: Placebo<br> | Dietary Supplement: Placebo oral capsule<br>* Subjects randomly assigned to the Placebo group will receive placebo oral capsules for 3 weeks<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Mitochondrial function | Measured via high-resolution respirometry using the Oroboros Oxygraph | 3 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Exercise Capacity | To measure maximal endurance capacity measured via VO2max | 3 weeks | 
| Muscle strength | To measure maximal voluntary contraction using a force transducer strength chair | 3 weeks | 
| Muscle power | Determine the effects of HIIT with and without NRPT supplementation on muscle power producing capabilities measured by the 30 second Wingate test | 3 weeks | 
| Mitochondrial proteins | To determine whether HIIT with NRPT supplementation improves mitochondrial function more so than HIIT alone, using Western blotting | 3 weeks | 
| Body Mass | To determine the effects of HIIT with and without NRPT supplementation on body composition by measuring body mass using a bioelectrical impedance analysis scale | 3 weeks | 
| Percent Body Fat | To determine the effects of HIIT with and without NRPT supplementation on body composition by measuring percent body fat using a bioelectrical impedance analysis scale | 3 weeks | 
| Recovery via POMS | To determine the effects of HIIT with and without NRPT supplementation on recovery via profile of mood states (POMS) | 3 weeks | 
| Recovery via RESTQ | To determine the effects of HIIT with and without NRPT supplementation on recovery via recovery stress (RESTQ) questionnaire | 3 weeks | 
| Physical activity levels using FitBit | To characterize volunteer's habitual physical activity levels. | 3 weeks | 
| Height | Measure height using a stadiometer, to characterize demographics of recruited volunteers the recruited volunteers | 3 weeks | 
| Blood pressure | Measure blood pressure using Dinamap Pro monitor to characterize demographics of recruited volunteers the recruited volunteers | 3 weeks |