Cryosurvival of Embryos From Dysmorphic Oocytes

Study Overview
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Brief Summary
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Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Conditions
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Cryopreservation, Blastocyst

Intervention / Treatment
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* Procedure: Cryopreservation, thawing and blastocyst culture


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure. Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle. Exclusion Criteria: Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture. Embryos obtained from abnormally fertilized zygotes.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Female

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Normal<br>Embryos derived from morphologically normal MII oocytes | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Irregular Shapes<br>Embryos derived from irregularly shaped oocytes. | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Large PVS<br>Embryos derived from oocytes with large perivitelline space. | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Dark Zona<br>Embryos derived from oocytes with dark zona pellucida | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Dark cytoplasm<br>Embryos derived from oocytes with dark cytoplasm | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Vacuolar cytoplasm<br>Embryos derived from oocytes with vacuolated cytoplasm | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Central granulation<br>Embryos derived from oocytes with centrally granulated cytoplasm | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Double extra<br>Embryos derived from oocytes with double extracytoplasmic abnormalities | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Double combined<br>Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Double cytoplasmic<br>Embryos derived from oocytes with any two cytoplasmic anomalies | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Triple extra<br>Embryos derived from oocytes with triple extracytoplasmic anomaly | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|
| Triple combined<br>Embryos derived from oocytes with triple combined anomalies | Procedure: Cryopreservation, thawing and blastocyst culture<br>* Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |