Analyses of Bone Marrow and Blood Samples From Healthy Volunteers: Focus on the redOx Metabolism

Study Overview
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Brief Summary
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Oxidative stress is defined by an excess of reactive oxygen species (reactive oxygen species or ROS) in cells. ROS are essential to cell life (proliferation, differentiation), including kinase activity via inhibition of phosphatases. Very low levels of ROS are observed in quiescent cells, including hematopoietic stem cells. Conversely, an excess of ROS can induce DNA damage that may lead to the destruction of the cell. The level of ROS in cells is the result of production (mainly the NADPHoxydase and the mitochondrial respiratory chain) and their disposal via antioxidant enzymes. The hematology team of the university hospital of Tours (France) found that certain antioxidant enzymes are essential for the self-renewal of normal and leukemic cells such as glutathione peroxidase-3 (Herault O et al, J Exp Med 2012, 209:895). Their expression is modified in hematological diseases (patent WO2012085188A1 / 2012-06-28 and FR1000267310 / 2014-11-27). Age potentially affects the level of expression of antioxidant genes, since it was established a decrease in glutathione peroxidase activity in women over 65 years (Sara E, J Gerontol A Biol Sci Med Sci 2008 63: 505). The study HEALTHOX will determine the impact of age on the oxidative metabolism of normal hematopoietic cells, and in particular the expression of antioxidant genes. It will provide a reference for analyzing disturbances of oxidative metabolism in blood diseases. The main objective is the comparison of peripheral blood and bone marrow cells depending from 3 groups of healthy volunteers: 18-39 years, 40-59 years and 60-85 years. The level of ROS and the expression of genes encoding the major antioxidant enzymes involved in the regulation of oxidative stress will be studied. The secondary objectives are to create a cell bank (blood and marrow), a blood plasma bank and a cDNA library (blood and marrow) from healthy volunteers.

Official Title
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Analyses of Bone Marrow and Blood Samples From Healthy Volunteers: Focus on the redOx Metabolism - HEALTHOX

Conditions
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redOx Metabolism

Intervention / Treatment
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* Procedure: Blood test
* Procedure: Medullar test


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy volunteers Age ≥ 18 years old and ≤ 85 ans Informed and written consent Affiliation to Social Security Exclusion Criteria: Subject with an history of hematologic disease Subject with an history of tumoral disease with chemotherapy or radiotherapy Subject with an history of infectious or inflammatory disease Subject taking a treatment in prevention of blood clots Subject under guardianship or protection measure Subject with in the last 30 days food supplement rich in antioxidant : ascorbic acid, trace elements, etc… Subject with in the last 30 days treatment with known effect on oxydative metabolism : N-Acetyl-Cysteine, etc… Participant to a drug study Pregnant woman

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Healthy volunteer<br>Blood test Medullar test | Procedure: Blood test<br>* Blood test of 30mL<br>Procedure: Medullar test<br>* Medullar test for 2-5mL and a smear<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of oxygene reactive species | Number of oxygene reactive species by flow cytometry | 12 months |