Medium Chain Triglycerides and Aerobic Exercise on Ketone Production in Women With or Without Prediabetes

Study Overview
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Brief Summary
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The aim of this study is to compare the effect on ketone production of dietary an MCT supplement combined or not with physical exercise in two groups of women (N=10/group), healthy adults and patients with prediabetes. After an evaluation of the metabolism (a 4-hour visit with multiple blood samples) at the beginning of the study, all the participants are taken for one-week the MCT supplement alone follow by a one-week period of taking the MCT supplement in combination with physical exercise. At the end of each period of time, the 4-hour visit for the evaluation of the metabolism is repeated.

Official Title
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Stimulating the Production of Ketones Through the Combined Use of Aerobic Exercise (AE) and Dietary a Medium Chain Triglycerides (MCT) Supplement

Conditions
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Prediabetic State

Intervention / Treatment
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* Dietary Supplement: MCT
* Dietary Supplement: MCT+ aerobic exercise
* Dietary Supplement: Aerobic exercise
* Dietary Supplement: Control day


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy women group: fasting blood glucose < 6.1 mmol/L and HbA1c < 6.0 %. Women with prediabetes: fasting blood glucose between 6.1 and 6.9 mmol/L and HbA1c between 6.0 and 6.4 % Exclusion Criteria: Structured physical activity (more than 2 times per week) Inability to practice physical exercise Diabetes (fasting glucose ≥ 7.0mmol/L or HbA1c ≥ 6.5%) Uncontrolled dyslipidemia or hypertension Use of medications known to affect the metabolism of lipids and carbohydrates (steroids, beta-blockers, diuretics, insulin sensitizers, etc.) Severe infections or inflammations

Ages Eligible for Study
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Minimum Age: 60 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Healthy women<br>Arm: Evaluation of MCT, aerobic exercise alone and MCT+ aerobic exercise on glucose/ketone/lipid/insulin metabolism in 4-hour visits with repeated blood sampling. Interventions: A control day with no MCT and no aerobic exercise. A 5 day consecutive MCT intake of 30 g/day. 30 minutes of aerobic exercise at 70% to 80% HRR of intensity. A 5 day consecutive MCT intake of 30 g/day in combination with 30 minutes of aerobic exercise (70% to 80% HRR)/ day for the five days. | Dietary Supplement: MCT<br>* A 5 day consecutive MCT intake of 30 g/day.<br>Dietary Supplement: MCT+ aerobic exercise<br>* A 5 day consecutive MCT intake of 30 g/day in combinaison with 30 minutes of aerobic exercise (70% to 80% HRR) per day for the five days.<br>* Other names: MCT+AE;Dietary Supplement: Aerobic exercise<br>* A single period of 30 minutes of aerobic exercise (70% to 80% HRR).<br>* Other names: AE;Dietary Supplement: Control day<br>* No MCT or aerobic exercise<br>* Other names: CTL;|
| Experimental: Women with prediabetes<br>Arm: Evaluation of MCT, aerobic exercise alone and MCT+ aerobic exercise on glucose/ketone/lipid/insulin metabolism in 4-hour visits with repeated blood sampling. Interventions: A control day with no MCT and no aerobic exercise. A 5 day consecutive MCT intake of 30 g/day. 30 minutes of aerobic exercise at 70% to 80% HRR of intensity. A 5 day consecutive MCT intake of 30 g/day in combination with 30 minutes of aerobic exercise (70% to 80% HRR)/ day for the five days. | Dietary Supplement: MCT<br>* A 5 day consecutive MCT intake of 30 g/day.<br>Dietary Supplement: MCT+ aerobic exercise<br>* A 5 day consecutive MCT intake of 30 g/day in combinaison with 30 minutes of aerobic exercise (70% to 80% HRR) per day for the five days.<br>* Other names: MCT+AE;Dietary Supplement: Aerobic exercise<br>* A single period of 30 minutes of aerobic exercise (70% to 80% HRR).<br>* Other names: AE;Dietary Supplement: Control day<br>* No MCT or aerobic exercise<br>* Other names: CTL;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma Ketone Concentrations | Acetoacetate (umol/L) and beta-hydroxybutyrate (umol/L); Called Ketones (µmol/L) when combine together The area under the curve (AUC) was calculated according to the trapezoidal method from 0 to 4 h during the visits (interventions) CTL = Metabolic study day (Day 1) MCT = Metabolic study day after 5 days of supplementation (Day 5) AE= Metabolic Study day after acute aerobic exercise (Day 6) AE + MCT = Metabolic Study day after 5 days of aerobic exercise and mct supplementation (Day 11) | 5 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma Glucose Concentrations | Glucose (mmol/L) measured in the plasma The area under the curve (AUC) was calculated according to the trapezoidal method from 0 to 4 h during the visits (interventions) CTL = Metabolic study day (Day 1) MCT = Metabolic study day after 5 days of supplementation (Day 5) AE= Metabolic Study day after acute aerobic exercise (Day 6) AE + MCT = Metabolic Study day after 5 days of aerobic exercise and mct supplementation (Day 11) | 5 days | 
| Plasma Triglyceride Concentrations | Triglycerides (mmol/L) measured in the plasma The area under the curve (AUC) was calculated according to the trapezoidal method from 0 to 4 h during the visits (interventions) CTL = Metabolic study day (Day 1) MCT = Metabolic study day after 5 days of supplementation (Day 5) AE= Metabolic Study day after acute aerobic exercise (Day 6) AE + MCT = Metabolic Study day after 5 days of aerobic exercise and mct supplementation (Day 11) | 5 days | 
| Plasma Free Fatty Acid Concentrations | Free fatty acids (mmol/L) measured in the plasma The area under the curve (AUC) was calculated according to the trapezoidal method from 0 to 4 h during the visits (interventions) CTL = Metabolic study day (Day 1) MCT = Metabolic study day after 5 days of supplementation (Day 5) AE= Metabolic Study day after acute aerobic exercise (Day 6) AE + MCT = Metabolic Study day after 5 days of aerobic exercise and mct supplementation (Day 11) | 5 days | 
| Plasma Insulin Concentrations | Insulin (nmol/L) measured in the plasma The area under the curve (AUC) was calculated according to the trapezoidal method from 0 to 4 h during the visits (interventions) CTL = Metabolic study day (Day 1) MCT = Metabolic study day after 5 days of supplementation (Day 5) AE= Metabolic Study day after acute aerobic exercise (Day 6) AE + MCT = Metabolic Study day after 5 days of aerobic exercise and mct supplementation (Day 11) | 5 days |