Influence of EPICardial Adipose Tissue in HEART Diseases: EPICHEART Study

Study Overview
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Brief Summary
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This translational study was designed to explore the association of the quantity and quality of epicardial adipose tissue (EAT) with coronary artery disease (CAD), left atrial remodeling and postoperative atrial fibrillation in a high cardiovascular disease-risk population. The investigators expect to identify new biochemical factors and biomarkers in the crosstalk between the epicardial adipocytes, coronary plaques and atrial cardiomyocytes that are involved in the pathogenesis of atherosclerosis and atrial fibrillation, respectively.

Detailed Description
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Background: EAT has emerged as a new independent, and, potentially, modifiable cardiovascular risk factor for CAD. EAT volume assessed by computed tomography (CT) was independently associated with the presence of coronary stenosis, coronary calcification and myocardial ischemia in cross-sectional studies, and, prospectively, with major adverse cardiovascular events. Most of these clinical studies were, however, derived from community-based patients with low-to intermediate-risk profile and the role of EAT in high-risk patients is currently unclear. Accumulation of EAT has been also associated with left atrial (LA) dilation, presence, chronicity, and recurrence of atrial fibrillation (AF). Although there is evidence suggesting that EAT may be a major determinant of the LA vulnerable substrate of AF, the mechanisms in the causal pathway between the EAT and LA remodeling are not completely elucidated. Aims: The main aims are to investigate if the volume of the EAT on CT and EAT proteome assessed by SWATH-mass spectrometry are associated with extent, distribution and complexity of coronary stenosis and coronary artery calcification, left atrial strain and incidence of postoperative atrial fibrillation in patients with symptomatic severe aortic stenosis. Methods: This a prospective study enrolling symptomatic severe aortic stenosis patients referred to aortic valve replacement. The protocol includes preoperative detailed clinical and nutritional evaluations, echocardiography, CT, cardiac magnetic resonance imaging and invasive coronary angiography. During cardiac surgery, biopsies from the EAT, mediastinal and subcutaneous thoracic adipose tissues will be performed to undergo analysis of proteome using SWAT-mass spectrometry. Samples from the pericardial fluid, circulating and coronary sinus blood samples will be collected as well in order to find local and peripheral adipose tissue-derived biomarkers of the disease.

Official Title
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Association of the Volume and Proteome of Epicardial Adipose Tissue With Coronary Artery Disease, Left Atrial Remodelling and Atrial Fibrillation in Severe Aortic Stenosis Patients

Conditions
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Coronary Artery Disease, Coronary Arteriosclerosis, Atrial Fibrillation, Left Atrial Abnormality, Severe Aortic Stenosis

Intervention / Treatment
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* Other: Aortic valve replacement


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: symptomatic severe aortic stenosis patients (defined as aortic valve area of < 1 cm2 or 0.6 cm2/m2 by transthoracic echocardiography) referred to aortic valve replacement. Exclusion Criteria: diagnosis of acute coronary syndrome in the last 3 months. prior history of persistent or permanent atrial or flutter fibrillation. coexisting moderate to severe aortic valve regurgitation or moderate to severe mitral valve disease, bicuspid aortic valve. left ventricular dilatation [end-diastolic volume index >75 mL/m²]. left ventricular ejection fraction <55%. chronic renal failure stage 3 to 5 defined as glomerular filtration rate GFR estimated by Cockcroft-Gault formula adjusted for body surface area < 30 mL/min/1.73m². moderate to severe chronic obstructive pulmonary disease defined as forced expiratory volume in one second <50% according to the 2011 Global Initiative for Chronic Obstructive Pulmonary Disease guidelines. active malignancy (i.e. With no evidence of recurrence and no longer receiving active treatment).

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: Aortic valve replacement|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| New onset atrial fibrillation | Incidence of atrial fibrillation after aortic valve replacement | Intra-hospital (i.e. from surgery until hospital discharge which means 7 days on average) | 
| Left atrial remodelling by transthoracic echocardiography and magnetic resonance imaging | Change in left atrial strain and volumes | 6-month following aortic valve replacement | 
| Frailty syndrome according to Fried et al. scale | Change in frailty syndrome classification | 6-month following aortic valve replacement | 
| Coronary artery disease according to the presence of coronary stenosis and/or calcification | Prevalent coronary artery stenosis and coronary calcification | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Left ventricular hypertrophy by transthoracic echocardiography and magnetic resonance imaging | Regression of left ventricular mass after aortic valve replacement | 6-month following aortic valve replacement | 
| Right ventricular structure and function by transthoracic echocardiography and magnetic resonance imaging | Changes in right ventricular structure and function after aortic valve replacement | 6-month following aortic valve replacement | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Epicardial adipose tissue, Coronary artery disease, Atrial fibrillation, Severe aortic stenosis, Proteome, Mass spectrometry, Computed tomography