Microarchitecture of Vitreoretinal Contact

Study Overview
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Brief Summary
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Investigation of the vitreoretinal contact microarchitecture in the macular and paramacular zones of the human retina.

Detailed Description
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Purpose: to study the features of the macular ILM, to study the features of the preretinal structures of the vitreous body of the Central retinal region, morphofunctional features of preretinal hyalocytes, and the mechanism of vitreoretinal adhesion. Study Protocol: examination of donor human eyes obtained from an eye Bank after sampling material for corneal transplantation. Research method: scanning electron microscopy (SEM) of the central retina (macular and paramacular zone): ILM, preretinal vitreous. Scientific hypothesis: vitreoretinal adhesion is provided not by laminin and fibronectin, but by the interweaving of vitreous collagen fibers between the epiretinal vitreous (more firmly fixed to the retinal surface) and the cortical vitreous. Expected results: determination of ILM thickness in the macular zone, microanatomy of preretinal vitreous strutcures, and possible mechanism of vitreoretinal adhesion, establishing the possibility of intravital synthesis of collagen fibers synthesis in the adult vitreous.

Official Title
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Microarchitecture of Vitreoretinal Contact

Conditions
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Vitreoretinal Detachment, Vitreo-Retinal Adhesion

Intervention / Treatment
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* Other: scanning electron microscopy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: eyes from donors without concomitant multiple organ pathology and ophthalmic pathology Exclusion Criteria: eye injury, age over 45 years, concomitant multiple organ pathology, previous eye diseases

Ages Eligible for Study
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Minimum Age: 35 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: cadaver eyes<br>The Central region of the retina and preretinal structures of cadaver eyes are examined | Other: scanning electron microscopy<br>* scanning electron microscopy of the сentral retina and preretinal structures<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| the ILM thickness in the macular zone (foveola) | the ILM thickness in the macular zone (foveola) in mkm by scanning electron microscopy data | within 3 days after receiving the cadaver eyes | 
| superficial microstructure of ILM | by scanning electron microscopy data | within 3 days after receiving the cadaver eyes | 
| features of the structural microarchitecture of the preretinal vitreous in the macular zone | by scanning electron microscopy data | within 3 days after receiving the cadaver eyes | 
| morphofunctional features of preretinal hyalocytes. | by scanning electron microscopy data | within 3 days after receiving the cadaver eyes | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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vitreomacular adhesion, vitreoretinal contact, vitreoretinal interface, hyalocytes, collagen, internal limiting membrane, human vitreous