Immune Profile of Acute VKH Patients PBMC

Study Overview
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Brief Summary
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The investigators will collect blood samples of different courses of VKH patients for investigating immune profile, observe major immune cells number ,functional and membrane molecular changes in the course of treatment, to investigate pathogenesis of VKH. Meanwhile, the investigators will collect clinical data of VKH patients to observe choroid and retina change in different courses.

Official Title
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Clinical Observational Research on Changes of Peripheral Blood Mononuclear Cell（PBMC） Immunity and Expression Profile in Peripheral Blood of Different Course of of Vogt-Hoyanagi-Harada（VKH） Patients.

Conditions
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VKH Syndrome

Intervention / Treatment
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* Drug: Steroids


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Acute VKH patients, meet a criterion of VKH, no recurrence. Age range from 18 to 50 years old. Exclusion Criteria: Patients with Severe Cardiovascular and Cerebrovascular Diseases Patients allergic to steroid or contrast agents; A history of ocular trauma or intraocular surgery within 3 months; Vaccinated; History of infectious diseases having a history of heredity, immune system correlation, neuroendocrine and digestive system diseases Patients who take health care products for a long time and have heavy alcohol and tobacco addiction.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| VKH patients<br>acute VKH patients | Drug: Steroids<br>* IV and oral steroids<br>|
| control<br>health people age/sex match to the VKH patients | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| T/B /NK(natural killer )/monocyte...etc, cell numbers of blood samples in VKH patients | use flow cytometry to count T/B /NK/monocyte...etc, cell numbers | one year | 
| T/B /NK/monocyte...etc cell subtype proportion in blood samples in VKH patients | use flow cytometry to measure T/B /NK/monocyte...etc cell subtype proportion | one year | 
| T/B /NK/monocyte...etc cell functional change in VKH patients | use ELISA(enzyme linked immunosorbent assay) to measure inflammatory cytokines level in peripheral blood | one year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| choroid and retinal thickness change in different course of VKH patients | Use OCT（optical coherence tomography） to measure choroid and retina thickness changes in macular zone of different course VKH patients | one year | 
| Use OCTA(optical coherence tomography angiography) to measure macular and optic disc vascular index | Use OCTA to measure macular and optic disc vascular index in different course VKH patients | one year | 
| Use Maia perimeter to measure macular integrality | Use Maia perimeter to measure macular integrality including light threshold and central fixation. | one year | 
| measure the Injury degree of choroid and retinal vessels | Use FFA(fluorescein fundus angiography)/ICG(Indocyanine Green Angiography) to measure the Injury degree of choroid and retinal vessels | one year | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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VKH; immune profile;