Electrochemistry Measurement of Skin Hydration Parameters

Study Overview
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Brief Summary
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To date measuring the effect of dermocosmetic products on the main identified factors of aging and alteration of the skin barrier is based on invasive and expensive experiments. Electrochemistry enables to measure a specific signal for a substance of interest e.g. Vitamin C using an surface contact with an electrode. Therefore, this study will evaluate the reliability and feasibility of measurements of skin's hydration parameters such as NMF and squalene using electrochemistry. These study will be made on three groups of individuals with different skin types: dry skin i.e. atopic dermatitis patients, oily skin i.e. acne skin and a control group of individual without facial dermatosis. Collects of parameters of interest will be made by using patch using electrochemistry (contact with an electrode and potentiostat to detect an electric signal) . It is a simple method that relies on a sensor / electrode pair that allows a study of the surface molecules of the skin. Application in the measurement of vitamin C in food products has already been validated. On a second hand, a collect by chromatography of the parameters of interest will be carried out in order to compare the new method with the reference method. This procedure has been developed via a procedure including collecting surface parameters using a patch an developping special electrodes and miniaturized detecting signal tool (potentiostat)

Official Title
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Pilot Study Assessing the Feasibility and Reliability of Measurements of Skin's Parameters Hydration by Electrochemistry

Conditions
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Acne, Atopic Dermatitis, Skin Electrochemistry

Intervention / Treatment
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* Other: Collects of skin's hydration


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 ≤ Age ≤ 70 Subjects with past history or follow-up for acne or rosacea stage I ou II, atopic dermatitis mild to moderate without immunosuppressive treatment Subjects enabling to perform a one day visit at the hospital Written consent according to local regulatory requirements Affiliated to a social security scheme Exclusion Criteria: Pregnant women or breastfeeding Patient under guardianship or curatorship, legal protection or protection of justice Participation in other interventional research on skin moisturizer

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: This study will include sixty subjects separate into three groups of twenty subjects based on the skin disease: acne, atopic dermatitis and a group control. This is a study validating the feasibility of a measure collected by a tool at the prototype stage with a view to pre-industrialization. The following process is: Measurement by patches and sensors of substances of interest on 2 areas of the face separated by the midline (forehead / cheek). Collection on the skin surface by 2 swabs repeated on 2 areas
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Atopic dermatitis patients<br> | Other: Collects of skin's hydration<br>* Collects of skin's hydration will be made by using patch using electrochemistry.<br>|
| Experimental: Patient with oily acne skin<br> | Other: Collects of skin's hydration<br>* Collects of skin's hydration will be made by using patch using electrochemistry.<br>|
| Active Comparator: Control group of patient without facial dermatosis<br> | Other: Collects of skin's hydration<br>* Collects of skin's hydration will be made by using patch using electrochemistry.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Feasibility of collecting an electrochemical measure of skin hydration parameter (natural moisturizer factor electric signal) at two different sites of the face at T0 using patch and electrode | Feasibility of collecting an electrochemical measure of skin hydration parameter (natural moisturizer factor electric signal) at two different sites of the face at T0 using patch and electrode. Signal detection (mVolt) or not using a patch to collect skin surface parameter applied on an electrode to collect and electric signal. | Day 0 or Day 1 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Validation of the measure process for different skin type e.g: dry, oily, normal | Rate of failure by site or skin type, obtained measure mean and SD for all and regarding each group | Day 0 or Day 1 | 
| Determine the correlation between electrochemistry measures or skin hydration (level of electrical signal mvolt ) and reference method: corneometry or chromatography. | Those two methods are routinely used to asses skin hydration using a swab contact then migration in a chromatography column or use of sebumeter Correlation coefficient between level of electrochemical measure (signal) regarding skin types and measures issued from reference methodology (corneometry or chromatography) | Day 0 or Day 1 | 
| Describe individuals' habits or daily routine for skin care using a questionnairemethodology (corneometry or chromatography) | Descriptive statistics: smoke, sun exposure, moisturizer use | Day 0 or Day 1 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Electrochemistry, Squalene, NMF, Skin