An Analysis of Peripheral Blood T Cell Subsets on Rheumatoid Arthritis

Study Overview
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Brief Summary
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To study the effects of T cell in peripheral blood of patients with RA undergoing selective B cell depletion have not been studied. We analyze the B and T cell subsets in patients with active RA treated undergoing this form of treatment with rituximab.

Detailed Description
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In this open labeled prospective study, we evaluate the therapeutic efficacy and peripheral blood cellular response of rituximab in 10 patients with active RA despite conventional DMARDs therapy. Patients are taking stable dose of methotrexate and at least 10mg folic acid per week for at least 4 weeks. Rituximab is administrated as 1000mg intravenous infusion on day 1 and day 15. Premedication as standard prescription consists of methylprednisolone 100mg IV, Chlorpheniramine maleate(piriton 10mg IV and oral paracetamol 500mg to be given 30 minutes before each infusion of rituximab. Oral prednisolone 60mg is tob e given from day 1-6 after rituximab infusion and 30mg from day 7-13. In patients who relapses following the first cycle can be repeated with the second cycle must fulfill the following conditions: Patients must have responded clinically to the first infusion with improvement Other disease modifying anti-rheumatic drugs (DMARDs) are not appropriate as determined by the investigators, either they have been on them before or are ineffective or due to side effects, Patients have a disease activity score DSA>2.6 At least 24 weeks since the first infusion Neutrophil count of >1.5 X 103/mcL The second infusion consists of rituximab 1000mg intravenous infusion on day 1 and day 15 as before and the same protocol of followed up every 4 weeks up to 52 weeks from the day of the first infusion.14 Premedication as standard prescription (consists of methylprednisolone 100mg IV, Chlorpheniramine maleate(piriton) 10mg IV and oral paracetamol 500mg to be given 30 minutes before each infusion of rituximab.

Official Title
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B Cell Depletion Therapy in Rheumatoid Arthritis: An Analysis of Peripheral Blood T Cell Subsets

Conditions
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Rheumatoid Arthritis

Intervention / Treatment
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* Drug: rituximab


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 21 or above Fulfilled the 1978 American college of Rheumatology(ACR) criteria for RA Seropositive for RF with RF>20 IU/ml Active disease despite treatment with at least 2 stable dose of DMARDs for at least 16 weeks, including MRX>10mg weekly 4 or more swollen and/or tender joints Stable dose of prednisolone<=12.5mg/day or NASID for at lease 4 weeks MTX>10mg/wk and folic acid>10 mg/wk for at lease 4 weeks Exclusion Criteria: Little or no ability for self-care Used a DMARD other than MTX(Leflunomide should be wash-out with cholestyramine 4 weeks prior screening) Received intra-articular,intramuscular, or intravenous corticosteroids in the last 4 weeks Concurrent treatment with any biologics within 8 weeks Infected joint prosthesis during the previous 5 years Autoimmune disease other than RA(except concurrent Sjogren's syndrome), active rheumatoid vasculitis, and history of systemic disease associated with arthritis, chronic fatigue syndrome. Serious infections, such as hepatitis, pneumonia, pyelonephritis in the previous 3 months Any chronic infectious disease such as renal infection, chest infection with bronchiectasis or sinusitis Recurrent bacterial infections with encapsulated organisms, primary or secondary immunodeficiency Active tuberculosis requiring treatment within the previous 3 years Opportunistic infection such as herpes zoster within the previous 2 months Any evidence of active cytomegalovirus;active Pneumocystis Jirovecl;or drug-resistant atypical mycobacterial infection Known hypersensitivity to murine proteins Current signs or symptoms of severe,progressive,or uncontrolled renal,hepatic,haematological,gastrointestinal,endocrine,pulmonary,cardias,neurological,or cerebral disease A history of lymphoproliferative disease including lymphoma or signs suggestive of disease,such as lymphadenopathy of unusual size or location(ie,lymphadenopathy nodes,in the posterior tangle of the neck,infraclavicular epitrochlear,or periaortic areas);splenomegaly Any know malignant disease except basal cell carcinoma currently or in the last 5 years

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: 1<br>Rituximab is administrated as 1000mg intravenous infusion on day 1 and day 15. | Drug: rituximab<br>* Patients are taking stable dose of methotrexate and at least 10mg folic acid per week for at least 4 weeks. Rituximab is administrated as 1000mg intravenous infusion on day 1 and day 15. Premedication as standard prescription consists of methylprednisolone 100mg IV, Chlorpheniramine maleate(piriton 10mg IV and oral paracetamol 500mg to be given 30 minutes before each infusion of rituximab. Oral prednisolone 60mg is tob e given from day 1-6 after rituximab infusion and 30mg from day 7-13.<br>* Other names: Mabthera;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The proportion of patients who achieved a response according to the ACR 20 response criteria at 24 weeks and 54 weeks after the initial infusion |  | wk52 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The proportion of patients who achieved a response according to ACR50,ACR70, physician's assessment of disease activity,patient's assessment of physical function by means of a health-assessment questionnaire(HAQ),quality of life measure by SF-36 |  | wk52 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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B-cell, rituximab, Rheumatoid Arthritis