Chronic Mycoprotein Consumption and Nucleotide Content in Metabolic Health

Study Overview
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Brief Summary
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Compare chronic (one week) of a 'normal' meat diet with one week of mycoprotein consumption, with or without a reduced nucleotide content, on markers of metabolic health, such as uric acid concentrations, blood glucose control and blood lipid profiles.

Official Title
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Effect of Chronic Mycoprotein Consumption, and of Its Nucleotide Content, in Uric Acid Concentrations and Other Markers of Metabolic Health in Adults

Conditions
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Healthy

Intervention / Treatment
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* Other: Animal protein based diet
* Other: Low-nucleotide mycoprotein based diet
* Other: High-nucleotide mycoprotein based diet


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI between 18 and 30 Exclusion Criteria: On medication (except contraception) Smokers Diagnosed with any metabolic or cardiovascular conditions BMI < 18 or > 30 Dietary restrictions (vegetarians/vegans, etc.)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Animal protein based diet<br>Seven day fully controlled normocaloric diet (1.2 g/ kg body weight of protein per day), with the main protein source at the two main meals (lunch and dinner) being provided by animal products (meat and fish). | Other: Animal protein based diet<br> <br> |
| Active Comparator: Low-nucleotide mycoprotein based diet<br>Seven day fully controlled normocaloric diet (1.2 g/ kg body weight of protein per day), with the main protein source at the two main meals (lunch and dinner) being provided by nucleotide-depleted mycoprotein products (commercial Quorn products). | Other: Low-nucleotide mycoprotein based diet<br> <br> |
| Active Comparator: High-nucleotide mycoprotein based diet<br>Seven day fully controlled normocaloric diet (1.2 g/ kg body weight of protein per day), with the main protein source at the two main meals (lunch and dinner) being provided by nucleotide-rich mycoprotein products. | Other: High-nucleotide mycoprotein based diet<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serum uric acid concentrations |  | Measured at baseline; days 2, 4 and 6 of the intervention; and after the 1 week intervention (day 8) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glycaemia and serum insulin - insulin sensitivity (oral glucose tolerance test) |  | Measured at baseline and after 1 week intervention (day 8) | 
| IL-6 |  | Measured at baseline and after 1 week intervention (day 8) | 
| 24 h blood glucose homeostasis - measured using a Dexcom CGMS |  | Measured 5 days at baseline (habitual data) and 5 days during the intervention (days 3 - 7) | 
| Metabolomics (using Nuclear Magnetic Resonance - NMR) |  | Measured at baseline; days 2, 4 and 6 of the intervention; and after the 1 week intervention (day 8) |