Effect of Oocyte Denudation Time and Intracytoplasmic Sperm Injection Time on Embryo Quality

Study Overview
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Brief Summary
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An observational analytic retrospective study was conduct using cross-sectional study. The subject were oocytes from in-vitro fertilization procedures using the ICSI method at the assisted reproductive technology clinic in a private hospital in Bandung for the period 2017 - 2019. Three variables were oocyte denudation time, ICSI time and embryo quality collected from samples that met the research criteria.

Detailed Description
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This study analyses the different oocyte denudation time after ICSI on all embryos generated during IVF procedures between 1 Januaryr 2017 and 31 December 2019. All oocytes arer categorised on how many hours their denudation times were and these were then assessed for their qualities.

Official Title
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The Effect of Oocyte Denudation Time and Intracytoplasmic Sperm Injection Time on Embryo Quality at Assisted Reproductive Technology Clinic

Conditions
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Fertility Disorders

Intervention / Treatment
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* Procedure: intracytoplasmic sperm injection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: oocytes collected from in-vitro fertilisation procedures involving intracytoplasmic sperm injection Exclusion Criteria: oocytes from patients >40 years old oocytes from primary infertility >5 years endometriosis polycystic ovarian syndrome

Ages Eligible for Study
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Minimum Age: 3 Hours
Maximum Age: 8 Hours

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| 3-4 hours denudation<br>oocytes with 3-4 hours denudation time | Procedure: intracytoplasmic sperm injection<br>* injection of sperm into an oocyte in assisted reproductive technology<br>* Other names: ICSI;|
| 4-5 hours denudation<br>oocytes with 4-5 hours denudation time | Procedure: intracytoplasmic sperm injection<br>* injection of sperm into an oocyte in assisted reproductive technology<br>* Other names: ICSI;|
| 5-6 hours denudation time<br>oocytes with 5-6 hours denudation time | Procedure: intracytoplasmic sperm injection<br>* injection of sperm into an oocyte in assisted reproductive technology<br>* Other names: ICSI;|
| 6-7 hours denudation time<br>oocytes with 6-7 hours denudation time | Procedure: intracytoplasmic sperm injection<br>* injection of sperm into an oocyte in assisted reproductive technology<br>* Other names: ICSI;|
| more than 7 hours denudation time<br>oocytes with more than 7 hours denudation time | Procedure: intracytoplasmic sperm injection<br>* injection of sperm into an oocyte in assisted reproductive technology<br>* Other names: ICSI;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oocyte denudation time | time measured for oocyte denudation | 3 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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infertility, assisted reproductive technology, embryo quality, oocyte denudation, intracytoplasmic sperm injection