Regeneration of Posterior Cruciate Ligament Injury Using Hypoxic Conditioned Allogenic Adipose Mesenchymal Stem Cell and Condition Medium

Study Overview
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Brief Summary
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Transplantation of Allogenic Adipose Mesenchimal Stem cell in hypoxic cultur condition with Ligament- derived conitioned medium can enhance regeneration of posterior cruciate ligament rupture

Detailed Description
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this study is in vivo study to prove effect of transplantation of Allogenic Adipose Mesenchimal Stem cell in hypoxic cultur condition with Ligament- derived conitioned medium in regeneration of posterior cruciate ligament rupture

Official Title
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Regeneration of Posterior Cruciate Ligament Injury Using Hypoxia Conditioned Allogenic Adipose Mesenchymal Stem Cell (AdMSC) and Ligament Derived Condition Medium (Secretome)

Conditions
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Adypose Mesenchimal Stem Cell, Ligament Rupture, Ligament Derived Conditined Medium

Intervention / Treatment
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* Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: white male new zealand rabbit weight 2500 - 3000 gram Exclusion Criteria: have no disease

Ages Eligible for Study
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Minimum Age: 2 Months
Maximum Age: 12 Months

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: control group<br>rabbit with posterior cruciate ligament rupture and treated with fibrin glue (sacffold) | Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium<br>* rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture and ligament derived conditioned medium<br>|
| Active Comparator: conditioned medium group<br>rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with ligament derived conditioned medium | Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium<br>* rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture and ligament derived conditioned medium<br>|
| Active Comparator: stem cells group<br>rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture condition | Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium<br>* rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture and ligament derived conditioned medium<br>|
| Experimental: composite group<br>rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture condition and ligament derived conditioned medium | Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium<br>* rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture and ligament derived conditioned medium<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| tensile strength of posterior cruciate ligament | measurement of tensile strength of posterior cruciate ligament | 6 weeks | 
| Thrombosite of Growth Factor - beta 1 | measurement value of Thrombosite Growth Factor - beta 1 | 6 weeks | 
| basic fibroblast of growth factor | measurement value of basic fibroblast of growth factor | 6 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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adypose mesenchimal stem cell hypoxic culture conditioned, Ligament Rupture, ligament derived conditined medium