Pathogenic Study of Adult Immune Enteropathies

Study Overview
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Brief Summary
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The study focuses the mechanisms underlying the loss of intestinal homeostasis in celiac disease, refractory celiac disease and other immune diseases such as monogenic enteropathy, inflammatory bowel diseases or drug induced intestinal diseases. Mechanisms of transformation of lymphocytes leading to onset of lymphomatous complications of immune enteropathies will be investigated. Mechanisms of loss of hepatic lymphocytic homeostasis will also be assessed in liver associated diseases.

Official Title
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Pathogenic Study of Adult Immune Enteropathies

Conditions
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Celiac Disease, Autoimmune Enteropathy, Inflammatory Bowel Diseases

Intervention / Treatment
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* Genetic: Blood sample collection; gastrointestinal biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 years or over affiliated to social insurance signed informed consent Exclusion Criteria: pregnancy under guardianship not able to sign informed consent

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active coeliac patients<br>Patients with active coeliac disease | Genetic: Blood sample collection; gastrointestinal biopsy<br>* Targeted Next generation sequencing and whole exome sequencing Phenotyping of intestinal lymphocytes, single cell analyses, cytokines dosage...<br>|
| Treated coeliac patients<br>Patients with coeliac disease on gluten free diet | Genetic: Blood sample collection; gastrointestinal biopsy<br>* Targeted Next generation sequencing and whole exome sequencing Phenotyping of intestinal lymphocytes, single cell analyses, cytokines dosage...<br>|
| Sprue type I<br>Patients with refractory coeliac disease of type I | Genetic: Blood sample collection; gastrointestinal biopsy<br>* Targeted Next generation sequencing and whole exome sequencing Phenotyping of intestinal lymphocytes, single cell analyses, cytokines dosage...<br>|
| Sprue type II<br>Patients with refractory coeliac disease of type II | Genetic: Blood sample collection; gastrointestinal biopsy<br>* Targeted Next generation sequencing and whole exome sequencing Phenotyping of intestinal lymphocytes, single cell analyses, cytokines dosage...<br>|
| Intestinal Lymphoproliferations<br>Patients with intestinal lymphoproliferations | Genetic: Blood sample collection; gastrointestinal biopsy<br>* Targeted Next generation sequencing and whole exome sequencing Phenotyping of intestinal lymphocytes, single cell analyses, cytokines dosage...<br>|
| Non coeliac enteropathies<br>Patients with non coeliac immune-mediated enteropathy | Genetic: Blood sample collection; gastrointestinal biopsy<br>* Targeted Next generation sequencing and whole exome sequencing Phenotyping of intestinal lymphocytes, single cell analyses, cytokines dosage...<br>|
| Patients without neoplastic or inflammatory intestinal disease<br>Patients without neoplastic or inflammatory intestinal disease | Genetic: Blood sample collection; gastrointestinal biopsy<br>* Targeted Next generation sequencing and whole exome sequencing Phenotyping of intestinal lymphocytes, single cell analyses, cytokines dosage...<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Intestinal Lymphocytes count | Lymphocytes isolation | Baseline | 
| DNA sequencing | Targeted Next Generation sequencing | Baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Celiac Disease, Autoimmune enteropathy, Primary immunodeficiency, Intestinal lymphoma, Liver autoimmune diseases