Metabolomic Embryo Profiles of Obese in Vitro Fertilization (IVF) Patients and Their Relationship With Polycystic Ovary Syndrome (PCO)

Study Overview
=================
Brief Summary
-----------------
The present study aims to elucidate if there is a metabolomic profile alteration in the embryos of obese women in order to understand if the reduced implantation rate observed in these patients is directly related to this factor. Furthermore, the investigators seek to establish if there is any difference between obese women with Polycystic Ovary Syndrome (PCO) and without PCO. The investigators compare these metabolomic profile embryos with embryos of egg-donation programme.

Conditions
-----------------
Infertility, Obesity

Intervention / Treatment
-----------------
* Other: Culture medium metabolomic analysis


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: BMI > 30 kg/m2 Age < 38 years old Non smokers First IVF cycle (groups 2 & 3, not Control group) Normal uterus (seen by ultrasound) normal basal hormonal profile(FSH,LH,estradiol,testosterone,androstenedione, insulin, SHBG, glucose, AMH) Antral follicle count(> 5 FA for each ovary) Menses every 25-35 days. Exclusion Criteria: < 5 or > 20 oocytes retrieved after stimulation cycle estradiol >3000 pg/ml or progesterone > 1,5 ng/ml on the hCG day. < 2 evolutive embryos in the culture medium.

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 38 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| CONTROL<br>Fertile women (egg-donors) | Other: Culture medium metabolomic analysis<br>* During the embryo transfer at day 3, the investigators keep and freeze a small drop of the culture medium of each embryo transferred. Each drop of culture medium will have a volume of 40-50 microliters, before freezing is spun at 10.000 rps to avoid any possible contamination. The frozen media culture is analyzed for the metabolomic profile.<br>|
| WITH PCO<br>Obese women with Polycystic Ovarian Syndrome | Other: Culture medium metabolomic analysis<br>* During the embryo transfer at day 3, the investigators keep and freeze a small drop of the culture medium of each embryo transferred. Each drop of culture medium will have a volume of 40-50 microliters, before freezing is spun at 10.000 rps to avoid any possible contamination. The frozen media culture is analyzed for the metabolomic profile.<br>|
| NO PCO<br>Obese women without Polycystic Ovarian Syndrome | Other: Culture medium metabolomic analysis<br>* During the embryo transfer at day 3, the investigators keep and freeze a small drop of the culture medium of each embryo transferred. Each drop of culture medium will have a volume of 40-50 microliters, before freezing is spun at 10.000 rps to avoid any possible contamination. The frozen media culture is analyzed for the metabolomic profile.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolic profile of culture media of Day 3 embryos | Determine differences in the metabolomic profile of the culture media of day 3 embryos, obtained after in vitro fertilization, according to the presence of obesity associated or not with polycystic ovary syndrome. | 3 days |