Vasopressin and V2 Receptor in Meniere's Disease

Study Overview
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Brief Summary
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Summary: Some of sicknesses are well known to be provoked by inadequate adaptation to physical and/or psychogenic stress in their daily life. Meniere's disease is also a common inner ear disease accompanied with vertigo, hearing loss and tinnitus especially in civilized people under stressed life style. Its oto-pathology was firstly revealed in 1938 to be inner ear endolymphatic hydrops through the temporal bone study. To elucidate the neuroscientific relationship between stress and inner ear, we examined plasma vasopressin (the anti-diuretic stress hormone) and its receptor, V2R in the endolymphatic sac (the inner ear endo-organ for endolymph absorption) in Meniere's patients.

Detailed Description
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Methods: Between 1998 and 2006, we enrolled and assigned 105 intractable Meniere's patients to examine plasma vasopressin during remission of vertigo attacks (controls: 30 patients with chronic otitis media). Using the real-time PCR method, we examined V2R mRNA expression in the endolymphatic sac in 12 patients (controls: 6 patients with acoustic neurinoma). By means of cell culture and cyclic AMP assay, we also examined cyclic AMP activity in the endolymphatic sac in 3 patients (controls: 3 patients with acoustic neurinoma).

Official Title
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Vasopressin and V2 Receptor in Meniere's Disease

Conditions
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Meniere Disease

Intervention / Treatment
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* Genetic: vasopressin, V2 receptor and cyclic AMP


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients were eligible for enrollment if they had received a clinical diagnosis of Meniere's disease according to the 1995 AAO-HNS criteria (Committee, 1995). These criteria can be briefly described as follows: Repeated attacks of vertigo: A definitive spell is spontaneous vertigo lasting at least 20 minutes. A mixed type of spontaneous nystagmus is observed during attacks. Fluctuating cochlear symptoms: The hearing test usually reveals a marked fluctuation of the threshold in the low and middle tone range. Exclusion Criteria: Any other neuro-otologic diseases.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: 1. Meniere's disease<br>Patients were eligible for enrollment if they had received a clinical diagnosis of Meniere's disease according to the 1995 AAO-HNS criteria (Committee, 1995). These criteria can be briefly described as follows: 1) Repeated attacks of vertigo: A definitive spell is spontaneous vertigo lasting at least 20 minutes. A mixed type of spontaneous nystagmus is observed during attacks. 2) Fluctuating cochlear symptoms: The hearing test usually reveals a marked fluctuation of the threshold in the low and middle tone range. | Genetic: vasopressin, V2 receptor and cyclic AMP<br>* plasma vasopressin, V2 receptor, cyclic AMP<br>* Other names: cyclic AMP;|
| No Intervention: 2. Acoustic neurinoma<br>Diagnosed by CT and/or MRI | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| plasma vasopressin level and V2 receptor mRNA in the endolymphatic sac in Meniere's disease vs controls |  | prospective | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| cyclic AMP activity in the endolymphatic sac in Meniere's disease vs controls |  | prospective | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Meniere's disease, stress, vasopressin, endolymphatic sac, V2 receptor, cyclic AMP, Plasma vasopressin level and V2R mRNA expression level in the endolymphatic sac both were significantly higher in patients with Meniere's disease., In Meniere's disease, there were significant negative co-relationships between vasopressin and V2R., The cyclic AMP activity was basically up-regulated in the endolymphatic sac in Meniere's disease.., The cyclic AMP sensitivity for vasopressin was significantly elevated in the endolymphatic sac in Meniere's disease.