Oxidative Stress as an Acute Exercise-induced Mechanism of Stem and Progenitor Cell Mobilization

Study Overview
=================
Brief Summary
-----------------
It is already known from literature that exercise mobilizes stem and progenitor cells into the peripheral blood. However, the exact mechanisms thereof remain to be fully elucidated. The investigators hypothesize that exercise-induced oxidative stress could be at least one of the responsible mechanisms and therefore want to study the exercise-induced stem and progenitor cell mobilization in a group of healthy young men when they exercise with, compared to when they exercise without antioxidative supplementation. The primary outcome is numbers of stem and progenitor cells in the peripheral blood after an acute bout of exercise. As a secondary outcome, numbers of apoptotic mature and immature cells in the blood will be analysed.

Official Title
-----------------
Effect of Oxidative Stress on Acute Exercise-induced Mobilization and Apoptosis of Hematopoietic, Endothelial and Mesenchymal Stem and Progenitor Cells and Mature Endothelial Cells in Healthy Young Male Subjects

Conditions
-----------------
Healthy

Intervention / Treatment
-----------------
* Dietary Supplement: Antioxidative supplementation


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Non-smoking Healthy Normal Body-Mass-Index (BMI): 18.5-24.9 kg∙m-2 Active (fulfill World Health Organization-guidelines for recommended levels of physical activity for adults aged 18 - 64 years) Willing to adhere to the study rules Exclusion Criteria: Known or suspected non-compliance, drug or alcohol abuse Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders etc. of the participant Previous enrolment into the current study Enrolment of the investigator, his/her family members, employees and other dependent persons Regular intake of medication Regular intake of antioxidant supplementations

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
-----------------
Male

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Interventional Model Description: Each participant enrolled in the study will be part of the supplementation and control condition group in a non-randomized order
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Antioxidative Supplementation<br>Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation | Dietary Supplement: Antioxidative supplementation<br>* Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in exercise-dependently mobilized stem and progenitor cell numbers in the peripheral blood with antioxidative supplementation | Numbers of hematopoietic, endothelial and mesenchymal stem and progenitor cells and mature endothelial cells in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation. | On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in numbers of exercise-induced apoptotic cells and oxidative stress markers in the peripheral blood with antioxidative supplementation | Numbers of apoptotic mononuclear cells, apoptotic hematopoietic, endothelial and mesenchymal stem and progenitor cells and apoptotic mature endothelial cells, as well as in oxidative stress markers in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation. | On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week) |