In Vivo Assessment of Glutamine Utilization by Bone Marrow Plasma Cells

Study Overview
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Brief Summary
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This pilot study will first establish the feasibility of an in vivo methodology of assessing the utilization of glutamine into the TCA cycle of normal bone marrow plasma cells from healthy subjects while comparing it to an ex vivo approach

Detailed Description
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Twenty healthy adult study participants between the ages of 18 and 60 years will be recruited to participate in this pilot study that will include two groups: a) Ex Vivo group (10 volunteers) and b) In Vivo group (10 volunteers). Volunteers will be recruited to either group in a sequential alternating fashion. In vivo 13C SIRM studies evaluating the utilization of glutamine into the TCA cycle of normal bone marrow plasma cells.

Official Title
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In Vivo Assessment of Glutamine Utilization by Bone Marrow Plasma Cells in Healthy Subjects Using Stable Isotope Resolved Metabolomics: A Pilot Study

Conditions
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Healthy

Intervention / Treatment
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* Other: 5-13C-Glutamine
* Other: Bone marrow aspiration


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Ages 18-60 Able to provide written consent Exclusion Criteria: Renal Failure Pregnancy Active steroid use Active liver Disease Anemia (Hemoglobin < 12.5 g/dL in men and < 11.5 g/dL in women) H/O alcohol use (average > 2 drinks per day) BMI > 30

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: In Vivo vs. Ex Vivo assessment of 5-13C-Glutamine incorporation by plasma cells via a bone marrow aspiration.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: In Vivo<br>Infusion of 5-13C-Glutamine intravenously in healthy subjects prior to undergoing a bone marrow aspiration. | Other: 5-13C-Glutamine<br>* Intravenous infusion of 5-13C-Glutamine<br>Other: Bone marrow aspiration<br>* Bone marrow aspiration of bone marrow aspirate<br>|
| Placebo Comparator: Ex Vivo<br>Healthy subjects will undergoing a bone marrow aspiration and then the plasma cells acquired will be cultured ex vivo in cell culture media containing 5-13C-Glutamine. | Other: Bone marrow aspiration<br>* Bone marrow aspiration of bone marrow aspirate<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Percentage of enrichment of 13-Carbon molecules in the TCA cycle metabolites of plasma cells measured by gas chromatography mass spectrometry. | The plasma cells obtained from the bone marrow aspiration will be processed to determine the percent of 13-carbon enrichment in the various molecules of the TCA cycle within the plasma cells. The outcomes will be reported as a percentage. E.G. 5% of glutamine is labeled with 13-Carbon; e.g. 4% of glutamate is labeled with 13-carbon etc. | Through study completion, an average of 1 hour |