Investigating the Effect of Obesity on Eosinophil Recruitment in Human Adipose Tissue

Study Overview
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Brief Summary
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Researchers are trying to better understand the relationship between inflammation in adipose tissue (AT) and insulin resistance.

Official Title
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Investigating the Effect of Obesity on Eosinophil Recruitment in Human Adipose Tissue

Conditions
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Obese, Healthy

Intervention / Treatment
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* Procedure: Fat Tissue Biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adults ages 30-50 years Non-smokers Lean subjects with a BMI of 25 or less, gender specific normal body fat, Obese, non-diabetic subjects with a BMI between 30 and 50 and taking no medications affecting glucose metabolism or lipid metabolism subjects scheduled for an elective laparoscopic or laparotomy abdominal surgery Exclusion Criteria: Receiving corticosteroid therapy or have a history of asthma, COPD or atopic syndrome taking any biologic treatment for any autoimmune disorder Taking any leukotriene receptor antagonist acting medications (ex. Montelukast) smoking

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Lean, Healthy Controls<br>Subjects with a body mass index (BMI) of 25 or less will have a fat tissue biopsy collected | Procedure: Fat Tissue Biopsy<br>* If subjects are scheduled for a surgical procedure a fat sample will be collected during the beginning of the surgery. If subjects are not scheduled for a surgical procedure, a small sample of subcutaneous (superficial) fat tissue from subjects abdomen area will be collected.<br>|
| Experimental: Obese, Non-Diabetic<br>Subject with a BMI between 30 and 50 kg/m2 will have fat tissue biopsy collected | Procedure: Fat Tissue Biopsy<br>* If subjects are scheduled for a surgical procedure a fat sample will be collected during the beginning of the surgery. If subjects are not scheduled for a surgical procedure, a small sample of subcutaneous (superficial) fat tissue from subjects abdomen area will be collected.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of eosinophils in adipose tissue | eosinophil number will be measured by flow cytometry in subcutaneous (superficial) adipose tissue | baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Correlation between eosinophil content and body mass index (BMI) | Measured as a linear regression between BMI (defined as the weight in Kg/height in square m; kg/m2) and eosinophil number in the fat | baseline |