Relationship Between LRG and Periodontal Disease

Study Overview
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Brief Summary
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This study aimed to investigate gingival crevicular fluid (GCF) and serum levels of LRG, interleukin (IL)-6 and tumor necrosis factor (TNF)-α in patients with periodontitis before and after non-surgical periodontal treatment.

Detailed Description
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Twenty-five stage 3 periodontitis and twenty-five periodontally healthy individuals were enrolled in the study. Clinical periodontal measurements were recorded; periodontitis patients received non-surgical periodontal treatment, and GCF and serum samples were obtained at baseline and at 6 weeks after treatment. LRG, IL-6 and TNF-α were determined by ELISA.

Official Title
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Leucin-rich alpha2 Glycoprotein (LRG): A Novel Acute Phase Protein Related With Stage 3 Periodontitis

Conditions
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Periodontitis, Acute-Phase Response, Inflammation

Intervention / Treatment
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* Other: Non-surgical periodontal treatment
* Other: Oral hygiene instructions


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 25-50 years of age having at least 20 natural teeth, excluding third molars having no systemic disease non-smokers criteria for healthy control group: BOP < 10% and PD ≤ 3 mm without clinical attachment loss or radiographic sign of alveolar bone destruction criteria for stage 3 periodontitis group: interdental CAL ≥ 5 mm at least 2 non-adjacent teeth, PD ≥ 6 mm and radiographic bone loss extending to the mid-third of the root or beyond. Exclusion Criteria: having any systemic diseases, smoking current pregnancy or lactation a history of periodontal treatment in the past 6 months using antibiotic, anti-inflammatory drugs or any other drugs within the past 6 months.

Ages Eligible for Study
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Minimum Age: 26 Years
Maximum Age: 49 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: stage 3 periodontitis<br>GCF and serum samples were collected before and after treatment from periodontitis patients. | Other: Non-surgical periodontal treatment<br>* Scaling and root planing (SRP), in four clinical visits<br>Other: Oral hygiene instructions<br>* Oral hygiene instructions includes brushing with the modified Bass technique, interdental cleaning with dental floss and interdental brushes.<br>|
| Placebo Comparator: Periodontally healthy controls<br>GCF and serum samples were collected from periodontally healthy controls at baseline for once. | Other: Oral hygiene instructions<br>* Oral hygiene instructions includes brushing with the modified Bass technique, interdental cleaning with dental floss and interdental brushes.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in the levels of biochemical parameters | The levels LRG, IL-6 and TNF-α were determined to define the diagnostic and prognostic potential as a biomarker in periodontitis. | Baseline and 6 weeks after treatment | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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periodontitis, gingival crevicular fluid, serum