Determination of the Bioavailability of Short Chain Fatty Acids in Healthy Humans

Study Overview
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Brief Summary
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The purpose of the study is to evaluate the bioavailability of acetate, propionate and butyrate in healthy humans using a stable isotope technology. In addition the level of acetate, propionate and butyrate production from inulin will be determined using the principle of isotope dilution.

Official Title
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Evaluation of the Bioavailability and Production of Short Chain Fatty Acids in the Colon. A Stable Isotope Study in Healthy Humans.

Conditions
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Short Chain Fatty Acids Bioavailability

Intervention / Treatment
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* Dietary Supplement: 13C-labeled Acetate
* Dietary Supplement: 13C-labeled propionate
* Dietary Supplement: 13C-labeled butyrate
* Dietary Supplement: Inulin


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy volunteers Regular dietary pattern (3 meals/day) Age: 18-65y BMI: 18,5-27 kg/m2 Exclusion Criteria: Intake of antibiotics 1 month prior to the study Abdominal surgery in the past, with the exception of appendectomy Intake of medication influencing the gastro-intestinal system 14 days prior to the study In treatment at a dietician Intake of pre- and/or probiotics Exposure to radioactivity 1 year prior to the study Serious chronic disease of the gastrointestinal tract Use of medication that affects the gastro-intestinal tract during the last 2 weeks prior to the study Pregnancy, pregnancy desire or lactation Blood donation during the last 3 months prior to the study Diabetes (type 1 or 2) Aberrant hemoglobin level (Hb) in blood. Normal between 14.0 and 18.0 g/dL for men and between 12.0 and 16.0 g/dL for women

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: 13C-labeled acetate<br> | Dietary Supplement: 13C-labeled Acetate<br>* 400 mg of sodium acetate 1-13C (2 colon delivery capsules with 200 mg)<br>|
| Experimental: 13C-labeled propionate<br> | Dietary Supplement: 13C-labeled propionate<br>* 340 mg of sodium propionate 1-13C (2 colon delivery capsules with 170 mg)<br>|
| Experimental: 13C-labeled butyrate<br> | Dietary Supplement: 13C-labeled butyrate<br>* 990 mg sodium butyrate 1-13C (2 colon delivery capsules with 495 mg)<br>|
| Experimental: Inulin<br> | Dietary Supplement: Inulin<br>* 15 g of inulin dissolved in 200 ml of water<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Concentrations of the short chain fatty acids (acetate, propionate and butyrate) in plasma and urine samples | Participants performed each 4 test days and they were followed each test day for 12 hours, with sample collections at regular time points. Blood samples were collected every hour during the first 4 hours and afterwards every 20 minutes during 8 hours. Urine samples were collected during 24h in different fractions: 0-4h, 4-8h, 8-12h, 12-24h. | 12 hours each test day and 4 test days/volunteer | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Short chain fatty acids, Acetate, Propionate, Butyrate, Bioavailability