Phase I / II Study of Enadenotucirev by Sub-acute Fractionated IV Dosing in Cancer Patients

Study Overview
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Brief Summary
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This is a multicentre, open-label, Phase I/II study of enadenotucirev in patients with either solid tumour of epithelial origin not responding to standard therapy or for whom no standard treatment exists (Phase I dose escalation stage Single cycle), mCRC not responding to standard therapy (Phase I dose escalation Repeat cycle cohort expansion stage ), mCRC not responding to standard therapy or advanced or metastatic bladder cancer not candidate for chemotherapy (Phase Ib) or mCRC in stable disease or partial response after 3-4 months of first line standard of care chemotherapy (Phase II).

Official Title
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A Clinical Study Of Enadenotucirev Administered by Sub-Acute Fractionated Intravenous Injection: Dose Escalation in Metastatic Epithelial Solid Tumours and Randomised Controlled Trial in Metastatic Colorectal Cancer

Conditions
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Solid Tumours of Epithelial Origin, Metastatic Colorectal Cancer, Metastatic Bladder Cancer

Intervention / Treatment
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* Biological: Enadenotucirev


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients must provide written informed consent Age ≥ 18 years and the patient must be at least the legal age limit to be able to give consent within the jurisdiction the study is taking place. ECOG performance status 0 or 1 Predicted life expectancy of 3 months or more Ability to comply with study procedures in the Investigator's opinion Recovered to Grade 1 from the effects (excluding alopecia) of any prior therapy for their malignancies Adequate renal function Creatinine ≤ 1.5 mg/dL or calculated creatinine clearance using the Cockcroft-Gault formula ≥ 60 mL/min, or measured creatinine clearance ≥ 60 mL/min, Haematuria: dipstick ≤ 2+ Proteinuria: dipstick ≤ 2+ Adequate hepatic function Serum bilirubin < 1.5 mg/dL Aspartate transaminase (AST) and alanine transaminase (ALT) ≤ 3x upper limit of normal (ULN) Adequate bone marrow function: Absolute neutrophil count (ANC) ≥ 1.5 x 109/L Platelets ≥ 100 x 109/L Haemoglobin ≥ 100 g/L for UCC and ≥ 90 g/L for other cancers Adequate coagulation tests: international normalised ratio (INR) ≤ 1.5 x ULN For females of childbearing potential (defined as <2 years after last menstruation or not surgically sterile), a negative serum pregnancy test must be documented within 14 days prior to first administration of study treatment For women who are not postmenopausal (24 months of amenorrhea) or surgically sterile (absence of ovaries and/or uterus): agreement to use two adequate methods of contraception, during the treatment period and for at least 3 months after the last dose of study drug For men: agreement to use a barrier method of contraception during the treatment period and for at least 6 months after the last dose of study drug At least 3 weeks since any dose of IV systemic chemotherapy and at least two weeks since any oral dose of capecitabine at the time of first administration of ColoAd1. Phase I Specific Inclusion Criteria: Dose Escalation Stage only (except Repeat Cycle Cohort): - Solid tumour of epithelial origin not responding to standard therapy or for whom no standard treatment exists Dose Expansion Stage Single Cycle and Dose Escalation Stage Repeat Cycle Cohort: mCRC not responding to standard therapy ≤ 3 prior lines of systemic therapy for advanced disease OR ≤ 4 prior lines of systemic therapy for advanced disease if one of the 4 lines was an anti EGFR therapy given as a single agent or combined to a previously administered chemotherapy regimen Phase Ib:mCRC not responding to standard therapy no more than 3 prior lines of systemic therapy for advanced disease OR no more than 4 prior lines of systemic therapy for advanced disease if one of the 4 lines was an anti EGFR therapy given as a single agent or combined to a previously administered chemotherapy regimen Advanced or metastatic UCC, who have received a maximum of one chemotherapy-containing regimen and a maximum of one other systemic treatment with biologic agents only. Phase II Specific Inclusion Criteria: mCRC Have received 3 - 4 months of first line chemotherapy with either FOLFOX, FOLFIRI or CAPOX, with or without bevacizumab At least one measurable lesion according to RECIST 1.1 criteria Documented partial response or stable disease Eligible to receive chemotherapy with FOLFOX or CAPOX after a short chemotherapy interruption (3 to 4 weeks) Exclusion Criteria for all Patients: Pregnant or breast feeding females; Known history or evidence of significant immunodeficiency due to underlying illness (e.g. human immunodeficiency virus [HIV]/acquired immunodeficiency syndrome [AIDS]) and/or medication (e.g. systemic corticosteroids, or other immunosuppressive medications including cyclosporine, azathioprine, interferons, within the past 4 weeks) Splenectomy Prior allogeneic or autologous bone marrow or organ transplantation Active infections requiring antibiotics, physician monitoring, or recurrent fevers >38.0 degrees centigrade associated with a clinical diagnosis of active infection Active viral disease or known positive serology for HIV, hepatitis B or hepatitis C; Use of the following anti-viral agents: ribavirin, adefovir, lamivudine or cidofovir within 7 days prior to day 1; or pegylated interferon (PEG-IFN) (within 14 days prior to first administration of ColoAd1) Administration of an investigational drug within 28 days prior to first dose of ColoAd1 Major surgery within 4 weeks or radiotherapy within 3 weeks prior to first dose of ColoAd1 Another primary malignancy within the past 3 years (except for non melanoma skin cancer or cervical cancer in situ) Central nervous system (CNS) metastasis that is symptomatic and/or requires treatment Any condition or illness that, in the opinion of the Investigator or the medical monitor, would compromise patient safety or interfere with the evaluation of the safety of the drug Known allergy to treatment medication or its excipients Any other medical or psychological condition that would preclude participation in the study or compromise ability to give informed consent Phase II Specific Exclusion Criteria: Progression on first line therapy A complete response on first line therapy Use of first line therapy for longer than 4 months Use of any first line treatment with a chemotherapy regimen other than FOLFOX, FOLFIRI or CAPOX (each with or without bevacizumab) More than 6 weeks since the last administration of 5 FU, capecitabine, oxaliplatin or irinotecan

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Enadenotucirev<br> | Biological: Enadenotucirev<br>* Oncolytic virus<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Phase 1 - Maximum Tolerated Dose | - Maximum tolerated dose (MTD) / maximum feasible dose (MFD) of enadenotucirev when administered by sub-acute fractionated IV injection (phase I Dose Escalation) and recommended dose for phase II. | Up to Day 22 | 
| Phase 1b - Selection of suitable schedule for repeat cycle IV administration | Open label assessment of 2 repeat cycle schedules, with expansion cohort at the MTD or MFD with best repeat cycle schedule in advanced/metastatic UBC patients. | Up to Day 134 |