The Effect of Resistance Exercise on Inflammation in Young and Older Adults

Study Overview
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Brief Summary
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Healthy young and older adults will complete two trials in a randomized crossover counter-balanced order, including resistance exercise and sedentary control. During each trial, blood samples will be collected. The investigators hypothesized that an acute bout of resistance exercise exert different inflammation responses in young and older adults.

Official Title
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The Acute Effect of High-load Resistance Exercise on Inflammation in Young and Older Adults

Conditions
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Aging, Inflammatory Response

Intervention / Treatment
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* Other: Resistance exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age: 20-80 Healthy male Without experience of resistance training Exclusion Criteria: No acute or chronic musculoskeletal symptoms Smoking Alcohol or drug abuse Fail to conduct resistance exercise

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: High-load resistance exercise<br>Subjects in high-load trial performed 3 sets per exercise, 8 repetitions with load of 70%-1RM with 90 sec of rest between sets, followed by a fourth set to voluntary failure. | Other: Resistance exercise<br>* 4 total resistance exercises that target all major muscle groups.<br>|
| No Intervention: Sedentary control<br>Subjects in control trial stayed sedentary during the period. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in biomarkers of inflammation | Biomarkers of inflammation will be measured such as IL-10 in pg/mL, IL-6 in pg/mL, TNF-α in pg/mL. | 24-hour during each study intervention | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in glucose | glucose in mg/dL | 24-hour during each study intervention | 
| Changes in metabolic parameters | Metabolic parameters will be measured such as TC in mg/dL, TG in mg/dL, and HDL in mg/dL. | 24-hour during each study intervention | 
| Changes in biomarkers of muscle damage | Biomarkers of muscle damage will be measured such as CK in U/L and LDH in U/L. | 24-hour during each study intervention | 
| Changes in molecular markers in peripheral blood mononuclear cells (PBMC) | Molecular markers in PBMC will be measured such as STAT3 in arbitrary units, IKK in arbitrary units, IkB in arbitrary units, p65 in arbitrary units, SOCS3 in arbitrary units, AMPK in arbitrary units, and SIRT1 in arbitrary units. | 24-hour during each study intervention | 
| Changes in complete blood count (CBC) | The white blood cell count and the count of each white blood cell type will be measured. | 24-hour during each study intervention | 
| Borgs Ratings of Perceived Exertion (RPE) | Borgs RPE is a method of estimating exertion rating, based on a 6 to 20 rating scale (6 being the no exertion at all, 20 being maximal exertion). | 1-hour during each exercise intervention |