Blood, Muscle and Exercise Responses to Sodium Bicarbonate Supplementation

Study Overview
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Brief Summary
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The aim of this study is to evaluate the changes in activity of transporters of H+ (monocarboxylate transporters; MCT1, MCT4, NHE) following sodium bicarbonate supplementation and subsequent exercise performance. Fifteen healthy men (age 18-35 years) will be invited to attend the laboratory on five separate visits to a randomized, double-blind, placebo-controlled study. Participants will be supplemented with a 0.3 g/kg dose of sodium bicarbonate or placebo (Analytical Pharmacy, Brazil) in opaque gelatin capsules. One hour after supplementation they will perform a 1-km time trial and every 10 minutes a blood sample will be collected. A muscle biopsy will be taken prior to supplementation, immediately prior to exercise, and immediately post-exercise. A questionnaire will be also used to evaluate the side-effects associated with sodium bicarbonate supplementation. Muscle and blood samples will be analysed for acidity and H+ and muscle samples will be analysed for H+ transporters (MCT1, MCT4, NHE).

Official Title
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Blood and Muscle Responses to Sodium Bicarbonate Supplementation and Subsequent Exercise Performance

Conditions
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Sports Nutritional Physiological Phenomena

Intervention / Treatment
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* Dietary Supplement: Sodium bicarbonate
* Dietary Supplement: Maltodextrin


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy males Exclusion Criteria: Smokers Chronic disease Prior or current use of steroids Use of creatine in the last 6 months Use of beta-alanine in the last 6 months

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Double-blind crossover
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Sodium bicarbonate<br>0.3 g∙kg-1body mass of sodium bicarbonate | Dietary Supplement: Sodium bicarbonate<br>* Sodium bicarbonate in powder form ingested in gelatine capsules. This leads to an increase bicarbonate concentration in blood, increasing buffering capacity which may lead to improvements in exercise<br>|
| Placebo Comparator: Placebo<br>Individuals will ingest 0.3 g/kg maltodextrin before undergoing exercise | Dietary Supplement: Maltodextrin<br>* Maltodextrin will be used as a placebo as it will not lead to any increases in circulating bicarbonate and, at this dose, will not likely have any physiological effect.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| H+ transporter activity | Change in activity of transporters related to H+ | 5 minutes Post-exercise | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Exercise performance | Change in 1 km cycling time-trial performance | 1 hour Post-supplementation | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Physiological Phenomena