The Effect of Water Intake on Glucose Regulation

Study Overview
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Brief Summary
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There is a growing body of evidence suggesting low water intake and elevated levels of the hormone vasopressin exacerbate glucose regulation. This project will examine the physiological mechanism by which low water intake impairs glucose homeostasis. In the current proposal we aim to: 1) quantify the degree of glucose impairment as a response to elevated vasopressin due to low water intake and 2) identify the physiological mechanism by which elevated vasopressin, as a response to low water intake, impairs glucose regulation

Official Title
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The Effect of Water Intake on Glucose Regulation: A Pilot Study

Conditions
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Glucose Metabolism Disorders, Dehydration (Physiology), Water Deprivation

Intervention / Treatment
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* Other: Water intake manipulation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI 27.5-35 Exclusion Criteria: BMI <27.5 or >35 high physical activity diabetes impaired liver or kidney function cardiovascular disease weight change of more than 3 kg in the past month pregnancy previous surgery on digestive tract (except appendectomy) fluid balance will be exclusionary such as serotonin re-uptake inhibitors.

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 55 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: High Water intake<br>5-days of High water intake according to IOM guidelines | Other: Water intake manipulation<br>* for 5 days prior to each experiment subjects will undergo manipulation of their water intake.<br>|
| Experimental: Low Water Intake<br>5-days of Low water intake of 500 mL/day | Other: Water intake manipulation<br>* for 5 days prior to each experiment subjects will undergo manipulation of their water intake.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| amount of glucose metabolized M | amount of glucose metabolized during the euglycemic & Hyperglycemic clamp | 220-240 min & 340-360 min of the protocol | 
| metabolic clearance rate for insulin | metabolic clearance rate for insulin during the euglycemic & Hyperglycemic clamp | 220-240 min & 340-360 min of the protocol | 
| quantity of glucose metabolized per unit plasma insulin concentration | quantity of glucose metabolized per unit plasma insulin concentration during the euglycemic & Hyperglycemic clamp | 220-240 min & 340-360 min of the protocol | 
| Rate of glucose appearance & disappearance | Rate of glucose appearance & disappearance during the euglycemic & Hyperglycemic clamp | 220-240 min & 340-360 min of the protocol | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma copeptin | Plasma copeptin in response to water manipulation | 0 min, 240 min & 360 min of the protocol | 
| glucagon | glucagon in response to water manipulation | 0 min, 240 min & 360 min of the protocol | 
| Cortisol | Cortisol in response to water manipulation | 0 min, 240 min & 360 min of the protocol | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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water, vasopressin