Interest of Molecular Analysis of Cerebral Thrombi in Determining the Prognosis and Etiology of Cerebral Infarction

Study Overview
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Brief Summary
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The MATISSE (Molecular Analysis of Thrombus for Ischemic Stroke prognosis and Etiology) project evaluates the hypothesis that the molecular composition of cerebral thrombus in metabolites, lipids, and proteins conditions the clinical prognosis at 3 months of the infarction and informs on its etiological subtype

Official Title
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Interest of Molecular Analysis of Cerebral Thrombi in Determining the Prognosis and Etiology of Cerebral Infarction

Conditions
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Ischemic Stroke

Intervention / Treatment
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* Other: Thrombi and blood analyses


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male or female 18 years of age or older; Patient with a cerebral infarction documented by brain imaging who received endovascular treatment by mechanical thrombectomy; Patient for whom it was possible to collect cerebral thrombus for LC-MS analysis during mechanical thrombectomy: Patient who was informed of the study and formulated a non-opposition to participation. If not, patient for whom a relative was informed and formulated a non-opposition. Exclusion Criteria: Patient with a cerebral infarction documented by cerebral imaging, having benefited from an endovascular treatment with mechanical thrombectomy that did not allow the extraction of a cerebral thrombus. Protected persons (articles L1121-5, L1121-6 and L121-8 of the Public Health Code): pregnant or breast-feeding women, persons deprived of liberty, under guardianship or curatorship

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patient suffering from ischemic stroke<br> | Other: Thrombi and blood analyses<br>* Thrombi and blood will be collected during thrombectomy<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determination and validation of molecular signatures, obtained by LC-MS analysis of cerebral thrombi, predictive of the excellent clinical prognosis | Rankin score (0 to 6, 6 meaning worse outcome) | 3 months after the cerebral infarction | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determination and validation of molecular signatures, obtained by LC-MS analysis | ASCOD (atherosclerosis, small vessel disease (SVD), cardioembolism, other and dissection) description (0 to 9, 9 meaning insufficient assessment to determine the presence or absence of the disease) | 7 days after the cerebral infarction | 
| Determination and validation of molecular signatures, obtained by LC-MS analysis | ASCOD (atherosclerosis, small vessel disease (SVD), cardioembolism, other and dissection) description (0 to 9, 9 meaning insufficient assessment to determine the presence or absence of the disease) | 3 months after the cerebral infarction | 
| Severe intracranial hemorrhage | Heildeberg classification (0 to 3d, 3d meaning subdural hemorrhage) | 3 months after the cerebral infarction | 
| Recovery of autonomy in walking | Parker score (0 to 9, 9 meaning better outcome) | 3 months after the cerebral infarction | 
| Recovery of autonomy in walking | Parker score (0 to 9, 9 meaning better outcome) | 7 days after the cerebral infarction | 
| Early Neurological Deterioration | Worsening of at least 4 points in the NIHSS (National Institutes of Health Stroke Scale) score (0 meaning better outcome, 0 to 42) | 24 hours after recanalization treatment | 
| Use of thrombolytic therapy in the acute phase | Quantification of thrombolytic therapy | Before thrombectomy | 
| Determination of molecular signatures predictive on successful recanalization after mechanical thrombectomy | Score mTICI (modified Thrombolysis In Cerebral Infarction, 0 to 3, 0 meaning worst outcome) | Right after the thrombectomy | 
| Positive infarct growth after successful recanalization | Quantification of infarctus volume by MRI | Between 24 and 48h after the thrombectomy | 
| Death | Death of the patient | 3 months after cerebral infarction | 
| Patient exposure to peak outdoor air pollution | Quantification of peak outdoor air pollution (identification of the location of the patient and the polluants observed in the areas) | 96 hours before cerebral infarction | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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ischemic stroke, thrombi, metabolites