Study of Leukocyte Populations in Patients With Chronic Inflammatory

Study Overview
=================
Brief Summary
-----------------
Exploration of pathophysiological mechanisms in chronic inflammatory rheumatism and rare systemic autoimmune diseases with the objective of identifying therapeutic targets.

Detailed Description
-----------------
The primary objective is to characterization of the quantitative and qualitative specificities of the different leukocyte sub-populations in patients with chronic inflammatory rheumatism and rare systemic autoimmune diseases. We will perform an exploratory descriptive study whom primary endpoint will be to assess by FACS the phenotype of the specific leukocyte subsets. In addition, we will characterize the protein and transcriptomic signature associated with the conditions for which we have obtained preliminary data showing their potential involvement in autoimmunity (i.e: IL7 pathway, IFN signature).

Official Title
-----------------
Study of Leukocyte Populations in Patients With Chronic Inflammatory

Conditions
-----------------
Systemic Autoimmune Disease, Chronic Inflammatory Rheumatism, Sjogren's Syndrome, Spondyloarthritis, Rheumatoid Arthritis, Systemic Lupus Erythematosus

Intervention / Treatment
-----------------
* Other: blood sample


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Age ≥18 years Patient who did not express his opposition Patients with chronic inflammatory rheumatism and rare systemic autoimmune diseases (pSS, lupus, scleroderma, myositis, unclassified connectivitis) diagnosed according to the usual criteria. Weight> 35 kg Exclusion Criteria: Corticosteroid treatment> 10 mg / day Pregnant patient Patients under legal protection Beneficiary of the state medical aid

Ages Eligible for Study
-----------------
Minimum Age: 18 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
No

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: blood sample|sampling of blood for research during routine care|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Characterization of the quantitative and qualitative specificities of the different leukocyte subpopulations in patients with chronic inflammatory rheumatism and rare systemic autoimmune diseases | Proportion of different leukocytes subset | At the end of the study (5 years) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Protein study (ELISA on serum) : IL7 | levels of IL7 and other cytokines | At the end of the study (5 years) | 
| Transcriptome study (RNA) : mRNA levels | assessement of different mRRNA involved in Il7/IFN pathways by molecular biology techniques (RNAseq, qPCR, nano string) | At the end of the study (5 years) | 
| Genomic study (DNA) : SNPs | Determination of the genotype of different SNPs involved in IL7/IFN pathways | At the end of the study (5 years) | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
leukocytes, macrophages, lymphocytes, rare systemic autoimmune diseases, chronic inflammatory rheumatism