The Importance of Blastocyst Diamater and Its Correlation to Reproductive Outcomes in PGT-A Cases

Study Overview
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Brief Summary
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Morphometric measurement of blastocyst diameter was taken on the day of trophoectoderm biopsy. PGT-A was done using NGS platform, followed by thawing and transfer of euploid blastocyts. Follow up of the Pregnancy and implantation rates is done and recorded to study the correlation between the diameter and reproductive outcomes of the blastocyst.

Detailed Description
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Blastocyst morphological grading proposed by Gardner and Schoolcraft including the expansion degree, inner cell mass, and trophoectoderm assessment has proved its importance. Blastocyst expansion degree that can presented in diameter is linked to the blastocyst implantation and overall success. In order to evaluate the importance of blastocyst diameter in PGT-A cases, measurements will be taken on the day of trophoectoderm biopsy TE (diameter in μm), followed by TE biopsy using flicking technique. Next, PGT-A will be done using NGS platform and results will be confirmed using PGT-ai. Thawing of euploid blastocyst will take place and expansion degree, survival rate will be recorded. Patient will be followed up for the clinical pregnancy and implantation rates.

Official Title
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The Importance of Blastocyst Diamater and Its Correlation to Reproductive Outcomes in PGT-A Cases

Conditions
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Infertility

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Female aged 18-45 years old Male aged 18-60 years old ICSI cases having PGT-A for their blastocysts Must have their blastocysts cultured in time lapse incubator Exclusion Criteria: Presence of any of the endometrial factors that affect embryo implantation such as hydrosalpings, adenomyosis or previous uterine infection Any contradictions to undergoing in vitro fertilization or gonadotropin stimulation Use of donor oocytes or sperm Use of gestational carrier

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blastocoele re-expansion degree | Defined as the degree of blastocoele re-expansion and presented in % | Two hours post thawing | 
| Pregnancy rate | Defined as clinical pregnancy per transfer | 15 days post embryo transfer | 
| Implantation rate | Defined as number of gestational sacs with fetal heart beat, shown by ultrasound in gestational week 6 over number of embryo transferred. | 6-8 weeks of pregnancy | 
| Blastocyst survival rate | Defined as the proportion of blastocysts that showed expansion degree ≥50% and their cells remained intact after thawing and re-expansion | Two hours post thawing | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Blastocyst diameter, PGT-A, Time lapse, TE biopsy, Blastocyst survival