Impact of Bean Polyphenols and Phytic Acid on Iron Absorption

Study Overview
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Brief Summary
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Recently isolated low PA varieties (lpa) in wheat, rice, maize, barley and beans might have the potential to alleviate iron bioavailability problems associated with PA. These plants have normal phosphate levels, but reduced PA phosphate due to various modifications of the biosynthetic pathway of PA. So far lpa crops are in an early stage of development, most of them exhibiting reduced yield and seed germination. However, the lpa bean isolated by Campion and co-workers 2009 by chemical mutagenesis exhibited only 10 % of the native bean PA concentration without any defects in terms of growth and yield. Crossing the lpa variety with bean varieties low in PP and high in iron might lead to the development of a variety which has the potential to improve the iron status of bean consuming populations. The following study will evaluate the relative impact of PA and PP on iron absorption from beans by comparing iron absorption from four different bean varieties. Iron absorption from a bean with normal PA concentration and high PP concentration will be compared to iron absorption from a bean with normal PA and low PP concentration as well as to two lpa varieties, one with high and one with low PP concentration.

Official Title
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A Stable Iron Isotope Study to Investigate the Impact of Phytic Acid and Polyphenols on Iron Absorption From Beans in Humans

Conditions
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Blood Iron Isotope Enrichment

Intervention / Treatment
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* Other: 586/8x87 white
* Other: 586/8x87 brown
* Other: BAT 881
* Other: 586/8


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Non-pregnant, non-lactating women Between 18 and 40 years Below 65kg Exclusion Criteria: Metabolic, chronic and gastro-intestinal disease Long-term medication Blood donation within 6 month before the study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: lpa with high polyphenol<br> | Other: 586/8x87 brown<br>* 2 x 50 g<br>|
| Experimental: lpa with low polyphenol<br> | Other: 586/8x87 white<br>* 2 x 50 g<br>|
| Experimental: control bean with low polyphenol<br> | Other: 586/8<br>* 2 x 50 g<br>|
| Experimental: control bean with high polyphenol<br> | Other: BAT 881<br>* 2 x 50 g<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Shift in iron isotopic composition in blood after consumption of different bean varieties |  | 1 month | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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beans, lpa mutant, polyphenols, phytic acid, stable iron isotopes