Alteration of Symbiosis Intestinal Microbiota on Patients With Anorexia Nervosa

Study Overview
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Brief Summary
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The purpose of this study will be to study the association between the level of psychic symptomatic of anorexia nervosa (AN) (intensity of food restriction, symptoms of anxiety and depression) and alteration of host environment symbiosis and the mechanism (dysbiosis of intestinal microbiota, increase of intestinal permeability, immunity alteration and low-grade inflammation).

Detailed Description
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This is a monocentric study aims to characterise the intestinal microbiota of anorexia nervosa patients with malnutrition, in comparison with the control subject, by DNA sequencing and metagenomic method. The interaction of intetinal microbiota with the host will be studied through the mecanistic studies and various parameters of alteration of symbiosis (intestinal permeability, inflammation) and their association with psychic symptoms of anorexia nervosa. The objective of this approche is to have not only a descrition of genomic of material of sampling, but also an overview of its potential functioning.

Official Title
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Study of Translational Process of Relationship Between Intestinal Microbiota of Dysbiosis and the Psychic Symptoms of Anorexia Nervosa (Eating Disorders and Anxio-depression Disorders)

Conditions
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Anorexia Nervosa, Dysbiosis, Anxious Depression

Intervention / Treatment
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* Biological: Stool sample


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: For patients: Patients aged ≥18 years; Anorexia according to DSM-5 and CIDI (Composite International Diagnostic Interview); Body mass index (BMI) (P/T2) < 15; Hospitalization for nutrition rehabilitation; Covered by a health insurance; Informed consent form signed. For Healthy Volunteers: Aged ≥18 years; 18.5 < BMI (P/T2) < 25; Covered by a health insurance; Informed consent form signed. Exclusion Criteria: For patients: Patients no-responding all criteria of DSM-5 or CIDI scores; Taken of antibiotic treatment 2 months / or laxativ 3 weeks before hospitalization; Somatic comorbidity should perturb intestinal microbiota (Crohn's disease, diabetes and all other chronic inflammatory diseases); Patients under guardianship; Patients covered by french AME scheme. For Healthy Volunteers: Any disease should perturb intestinal microbiota; Recent ponderal variation; Taken of antibiotic treatment 2 months or laxativ 3 weeks before hospitalization; Under guardianship; Covered by french AME scheme.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Anorexia patients<br>Hospitalized anorexia patients with body mass index < 15. | Biological: Stool sample<br>* Stool sample<br>|
| Healthy controls<br>Health volunteers: 18 years or older with body mass index between 18.5 and 25. | Biological: Stool sample<br>* Stool sample<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Eating Disorder | By Eating Disorder Inventory (EDI-3) | at baseline | 
| Anxio-depressive symptomatologic assessements by HAD score | By HAD score | at baseline | 
| Anxio-depressive symptomatologic assessements by scale of Beck BDI 13 | By scale of Beck BDI 13 | at baseline | 
| Anxio-depressive symptomatologic assessements by LSAS Lieboweitz social anxiety scale | By LSAS Lieboweitz social anxiety scale | at baseline | 
| Anxio-depressive symptomatologic assessements by MOCI | By Maudsley Obsessions and compulsions inventory (MOCI). | at baseline | 
| Anxio-depressive symptomatologic assessements | By HAD score, scale of Beck BDI 13, LSAS Lieboweitz social anxiety scale, Maudsley Obsessions and compulsions inventory (MOCI). | at baseline | 
| Physical exercise | By Global physical activity questionnaire (GPAQ) | at baseline | 
| Biological parameter | By serum serotonin level | at baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Diversity indice | By index de Sympson et Shannon | at baseline | 
| Intestinal permeability | By serum zouulin level | at baseline | 
| Immunity alteration | By interleukin 6 level | at baseline | 
| Liver function | Cytolyse analysis in serum | at baseline | 
| BMI | Calculate of BMI | at baseline | 
| Intestinal functional disorders | By Francis scale | at baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Anorexia nervosa, Dysbiosis, Anxious depression, Intestinal microbiota, Body mass index