Changes in Serum Fibroblast Growth Factor 21 (FGF21) Levels Correlated With Exercise

Study Overview
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Brief Summary
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We evaluated the effect of two weeks of intensive supervised physical activity in serum fibroblast growth factor 21 (FGF21) levels.

Detailed Description
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Comparative and longitudinal study. Anthropometric and biochemical evaluation were done before, 1hr and 4hr after a bout of exercise and repeated after two weeks of daily supervised exercise. We studied sedentary young healthy women. A treadmill exercise test (following the Bruce's protocol) was done five times per week (from Monday to Friday) for two weeks. The Department of Endocrinology and Metabolism at the INCMNSZ performed all biochemical laboratory measurements using standardized procedures.

Official Title
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Changes in Serum Fibroblast Growth Factor 21 (FGF21) Levels After a Cardiorespiratory Exercise Program

Conditions
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Sedentary Lifestyle

Intervention / Treatment
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* Other: Supervised Exercising


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Women Aged 18 to 35 years old, Body mass index (BMI) <30 kg/m2 Without contraindication for exercising Exclusion Criteria: Metabolic syndrome Diabetes Dyslipidemia Hypertension Asthma Thyroid disease Treated with fibrates, beta agonists or blockers History or current arrhythmias, murmurs, cardiomegaly, or treatment for cardiovascular diseases - Contraindications for exercise testing were also considered as exclusion criteria (see references)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Supervised exercising<br>A treadmill exercise test (following the Bruce's protocol) was done five times per week (from Monday to Friday) for two weeks to participants. | Other: Supervised Exercising<br>* A treadmill exercise test (following the Bruce's protocol) was done five times per week (from Monday to Friday) for two weeks.<br>* Other names: Bruce's protocol;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change From Baseline Fibroblast Growth Factor 21 (FGF21) Acutely and at Two Weeks | Serum measurement of FGF21 using human ELISA kit was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise. | Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change From Baseline Free Fatty Acids (FFAs) Acutely and at Two Weeks | Serum measurement of free fatty acids (FFAs) was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise. | Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | 
| Change From Baseline Epinephrine Acutely and at Two Weeks | Serum measurement of epinephrine was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise. | Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | 
| Change From Baseline Leptin Acutely and at Two Weeks | Serum measurement of leptin was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | 
| Change From Baseline Total Adiponectin Acutely and at Two Weeks | Serum measurement of total adiponectin was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | 
| Change From Baseline Fasting Glucose Acutely and at Two Weeks | Serum measurement of glucose was done at baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | Baseline, 1hr and 4 hr after a bout of exercise, and repeated after two weeks of daily supervised exercise | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Glucose, Fibroblast growth factor 21 (FGF21), Exercise, Metabolic equivalents (METs), Lipolysis, Free fatty acids (FFAs), Epinephrine