Comparison of the Effect of A-PRF and L-PRF on Outcomes After Removal of Impacted Mandibular Third Molar

Study Overview
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Brief Summary
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In this study, it was aimed to investigating and comparing the postoperative effects of leukocyte- and platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin (A-PRF) in terms of pain, swelling on the cheek, and trismus after mandibular third molar surgery. The study included a total of 27 patients with bilateral impacted mandibular third molar which surgically operated at different times. Patients were evaluated in two randomly separated groups. For the first group; A-PRF and for the second group; L-PRF was applied into the tooth socket. The outcomes variables were; pain, swelling, the number of analgesics taken, and trismus. These variables were also assessed on the basis of; 1st, 2nd, 3rd and 7th days following the operation.

Official Title
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Comparison of the Effect of Advanced Platelet-rich Fibrin and Leukocyte- and Platelet-rich Fibrin on Outcomes After Removal of Impacted Mandibular Third Molar: A Randomised Split-mouth Study

Conditions
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Pain, Postoperative, Trismus, Swelling

Intervention / Treatment
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* Genetic: Advanced Platelet Rich Fibrin (A-PRF)
* Genetic: Leukocyte- and platelet-rich fibrin (L-PRF)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age≥18 the presence of bilaterally symmetrically impacted mandibular third molar, which requires an extraction for prophylactic reasons absence of systemic diseases absence of chronic opioid taken; not being pregnant not smoking and no alcohol usage the absence of allergy to penicillin or any other drugs used during standardized postoperative therapy. Exclusion Criteria: tooth needed to be sectioning during the operation operation time exceeding 30 minutes the cases with severe periodontal disease or acute pericoronitis, the cases using antibiotics for an existing infection the cases not capable of following postoperative instructions

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 26 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: patients with bilateral impacted mandibular third molar which surgically operated at different times
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Advanced Platelet Rich Fibrin (A-PRF)<br>A-PRF was applied into the tooth socket after mandibular third molar surgery. | Genetic: Advanced Platelet Rich Fibrin (A-PRF)<br>* blood samples collected in 10 ml of vacuumed plain glass tubes were centrifuged for 14 minutes at 1500 rpm without anticoagulant agents in order to obtain A-PRF<br>|
| Active Comparator: Leukocyte- and platelet-rich fibrin (L-PRF)<br>L-PRF was applied into the tooth socket after mandibular third molar surgery. | Genetic: Leukocyte- and platelet-rich fibrin (L-PRF)<br>* blood samples collected in 10 ml of glass coated plastic tubes were centrifuged without anticoagulant agents for 10 minutes in order to obtain L-PRF<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Pain Scores Evaluted | For the postoperative period, the pain scores change was evaluated with the help of the visual analog scale (VAS) ranging from 0 (no pain) to 10 (most severe pain). | 1st day after the operation. | 
| Pain Scores Evaluted | For the postoperative period, the pain scores change was evaluated with the help of the visual analog scale (VAS) ranging from 0 (no pain) to 10 (most severe pain). | 2nd day after the operation. | 
| Pain Scores Evaluted | For the postoperative period, the pain scores change was evaluated with the help of the visual analog scale (VAS) ranging from 0 (no pain) to 10 (most severe pain). | 3rd day after the operation. | 
| Pain Scores Evaluted | For the postoperative period, the pain scores change was evaluated with the help of the visual analog scale (VAS) ranging from 0 (no pain) to 10 (most severe pain). | 7th day after the operation. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of Analgesics Taken by the Patients |  | Each patient was evaluated on the 1st, 2nd, 3rd, and 7th days after the operation. | 
| Trismus | Trismus was assessed by measuring the distance between the mesioincisal corner of the maxillary and mandibular right incisors while mouth was fully opened | Each patient was evaluated in order to obtain measurements on the 1st, 2nd, 3rd, and 7th days after the operation. | 
| Swelling on the cheek | In order to record swelling, a modification of the tape measurement method | Each patient was evaluated in order to obtain measurements on the 1st, 2nd, 3rd, and 7th days after the operation. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Leukocyte- and platelet rich fibrin, Advanced platelet rich fibrin, Impacted mandibular third molar surgery