Expression of miR-204 and Consequences on Capillarization in Limb Muscles of Patients With COPD

Study Overview
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Brief Summary
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Limb muscle dysfunction, characterized by loss of capillaries, is amongst the most troublesome systemic consequences of chronic obstructive pulmonary disease (COPD) leading to poor functional status and premature mortality. One prevailing hypothesis stipulates that modification in the expression of miR-204 leads to change the regulation of angiogenesis in vastus lateralis of patients with COPD when compared to controls.

Detailed Description
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The investigators objective is to investigate the impact of COPD on key signaling pathways involved in the muscle angiogenesis. Mild to severe COPD patients and healthy controls will be recruited. All of the investigators patients will undergo a thorough baseline assessment in pulmonary capacity and body composition. Blood sampling and biopsy of the quadriceps will be done. Key proteins and microRNAs of signaling intramuscular pathways involved in angiogenesis will be measured in the quadriceps.

Official Title
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Expression of miR-204 and Consequences on Capillarization in Limb Muscles of Patients With Chronic Obstructive Pulmonary Disease

Conditions
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Chronic Obstructive Pulmonary Disease

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: COPD (FEV1/FVC < 0.70) Healthy subjects: no evidence of airways obstruction (FEV1/FVC > 0.70 and FEV1 > 0.80 predicted) Smoking history > 10 pack-years Ex-smoker >6 months Exclusion Criteria: Chronic hypoxemia and/or hypercapnia (PaO2 < 60 mmHg or SpO2 < 88% at rest and/or PaCO2 > 45 mmHg) Recent exacerbation or oral corticosteroids (< 2 months) Recent cancer (< 3 years) Diabetes Myopathy, neuromuscular, neurological or articular diseases Kidney disease Other respiratory diseases Unstable cardiac disease Physical activity score > 9 in the Voorips questionnaire BMI > 30

Ages Eligible for Study
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Minimum Age: 50 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| COPD patients<br>Quadriceps biopsies will be done. No drug administration. | |
| Healthy controls<br>Quadriceps biopsies will be done. No drug administration. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| miR-204 in limb muscles in patients with COPD | i)To measure miR-204 level in the quadriceps of patients with COPD in comparison to controls. ii) To study possible involvement of miR-204 on pathways regulating angiogenesis and on muscle microcirculation in these individuals. | At baseline (COPD patients in a stable state of the disease) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| microRNAs in limb muscles of patients with COPD | i) To measure microRNAs levels in the quadriceps of patients with COPD in comparison to controls. ii) To study possible involvement of these microRNAs on pathways regulating myogenesis, angiogenesis and on muscle microcirculation in these individuals. | At baseline (COPD patients in a stable state of the disease) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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MicroRNAs, Angiogenesis, Skeletal muscle, COPD