Influence of Daily Activities on Retinal Microcirculation

Study Overview
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Brief Summary
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The daily behaviors such as drinking coffee, wine, reading, exercise and body postural changes may have influence on the function or morphology of the retinal microcirculation in healthy people.A recent study found that the fingertips microvascular bed constricted obviously after the intake of a cup of coffee with caffeine, it significantly reduced blood flow to the fingers; Long reading of electronic products can induce significant dry eye symptoms, affect visual quality, and cause asthenopia. Microcirculation is critical for regulating vascular resistance and blood flow in organs. Fundus blood vessels is the only visible microvascular in vivo, its morphology and function changes not only associated with eye disease, but also with cardiovascular and cerebrovascular diseases. Optical coherence tomography angiography (OCTA) is a new technique which could acquire blood flow information in a non-invasive way without the use of dye. Magnetic resonance imaging (MRI) can quantitatively evaluation of cerebral blood flow and retina-choroid blood flow using the noninvasive arterial spin labeling technique in a large field of view (FOV) without depth limitations.The study tried to detect the influence of daily behaviors such as drinking coffee, red wine, reading ,and exercise on the retinal microcirculation with OCTA and MRI, and provide reference for health behaviors.

Official Title
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The Influence of Daily Activities on Retinal Microcirculation in Normal Adults

Conditions
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Retinal Microcirculation Disorder

Intervention / Treatment
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* Behavioral: Beverage consumption
* Behavioral: Daily reading
* Behavioral: Body motion


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria Healthy adults The age range was from 18 to 25 years old Without another eye disease besides ametropia The spherical equivalent is equal or above minus 3.00 diopters No smoking or drinking Average daily caffeine consumption no more than 1 cup of coffee No diabetes, hypertension, heart disease, liver or kidney dysfunction No medication taken recently Exclusion Criteria Mental disorders such as depression and anxiety Intellectual disabilities Female in menstruation, gestation period, perinatal period or lactation period Unable to cooperate with eye examinations and test procedures Heart disease, hypertension, diabetes, liver and kidney dysfunction and other chronic diseases Patients with fever, acute infectious diseases Other conditions that are not suitable for drinking alcohol or coffee, such as allergic to alcohol or coffee

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 25 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Influence of beverage consumption 30 participants drink coffee, red wine, and water respectively on three mornings interval with a one-week washout period. Each period lasts 2 hours. OCTA and MRI scanning are taken every 30 minutes, and venous blood was collected before MRI scanning. Influence of daily reading 30 participants read paper books, play video games with mobile phones, and non-read respectively on three mornings interval with a one-week washout period. Each period lasts 8 hours. OCTA and MRI scanning are taken every 2 hours. Influence of body motion (exercise and body postural changes) Exercise: 30 participants will be enrolled. Participants undergo to jogging for 10 minutes, 30 minutes, and 1 hour respectively on three mornings interval with a 3-day washout period. OCTA and MRI scanning are taken before and after jogging. Body postural changes: 30 participants will be enrolled. OCTA and MRI scanning are taken before and after body postural changes.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Beverage consumption<br>Drink 300ml red wine (alcohol concentration:12.5%), 300ml coffee (caffeine concentration 72 mg), or 300ml water. | Behavioral: Beverage consumption<br>* The participants drink 300ml wine (alcohol concentration:12.5%)or 300ml coffee (caffeine concentration 72 mg), or water.<br>|
| Experimental: Daily reading<br>Reading paper books, playing video games with mobile phones, or non-reading. | Behavioral: Daily reading<br>* Reading paper books, playing video games with mobile phones, or non-reading for 8 hours.<br>|
| Experimental: Body motion<br>Exercise and body postural changes. | Behavioral: Body motion<br>* Exercise or body postural changes<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Vessel diameter index (VDI) | VDI is calculated using both the vessel area map and the skeletonized vessel map, to yield the averaged vessel caliber. | 3 weeks | 
| Vessel area density (VAD) | VAD is calculated as a unit-less ratio of the total image area occupied by the vasculature to the total image area in the binary vessel maps. | 3 weeks | 
| Flow impairment region (FIR) | FIZ: calculated by summing all 'no-flow' pixels up. | 3 weeks | 
| Retina-choroid blood flow | Blood flow for the entire retinal thickness were determined as a function of the distance from the optic nerve head, where the blood flow was measured at the peaks of the projection profiles. An region of interest outlining the posterior retina with a size of 0.7 to 0.8 mm across the retina-choroid thickness in the parafoveal region was used to obtain the blood flow signal time courses and averaged the values. | 3 weeks | 
| Cerebral blood flow (CBF) | The frontal lobe was selected as the region of interest (ROI) to measure and average the changes in CBF | 3 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Beverage consumption, Reading, Body motion, Retinal microcirculation, Optical coherence tomography angiography, Magnetic resonance imaging