MenPF-1 - A New Vaccine Against Meningococcal Disease

Study Overview
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Brief Summary
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In this study the investigators are testing a new vaccine against Neisseria meningitidis, the leading infective cause of childhood death in the UK. This bug (also known as meningococcus) can infect the lining of the brain (meningitis) or the blood stream (septicaemia) and can affect all ages, but especially children, adolescents and young adults. The bug is classified into different groups based on its outer capsule (or shell), and this study will test a new vaccine to protect against group B meningococcus (MenB) disease, which is the most common type in the UK. Vaccines are given to prepare the immune system to fight an infection. Vaccines work by stimulating the immune system to produce specialised proteins (called antibodies) and white blood cells designed to kill the bug later in life if needed. Vaccines against other types of meningococcus have been developed and saved many lives. However MenB is different because its outer capsule does not stimulate the immune system very effectively. There is therefore no broadly effective vaccine against MenB disease.

Detailed Description
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The vaccine the investigators are testing in this study is known as MenPF-1, and uses two bacterial proteins called PorA and FetA. PorA works to move particles across the bacterial cell wall, and FetA is needed to bind to iron that the bacteria need in order to grow. These proteins are found on almost all meningococcal bugs so they are an excellent target for the immune system, and it is thought that they could be the key to developing a new vaccine against MenB disease. MenPF-1 is a type of vaccine known as an Outer Membrane Vesicle (OMV) vaccine. This means that it has been produced from 'blebs' of the meningococcal bug's outer membrane, released by the meningococcus both during natural infection and in the laboratory. These outer membrane vesicles contain the PorA and FetA proteins, so can be used to make vaccines. The techniques used to produce the OMVs to make MenPF-1 are the same as those used in other very similar safe and effective vaccines. The MenB strain that the investigators use naturally produces PorA protein, and has been genetically modified to produce increased amounts of FetA for the OMV vaccine. MenPF-1 contains: Many proteins, but especially PorA and FetA Aluminium, which is safe and commonly used in many vaccines to better activate the immune system Sugar and water for injection It is worth noting that the vaccine product MenPF-1 is designed to work against MenB and, even if successful, would not protect against all forms of meningitis. Also, it is not a live vaccine and therefore cannot cause a meningitis infection.

Official Title
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A Phase I, Single Centre, Open-label Dose-escalation Study to Assess the Safety and Immunogenicity of Three Doses of 25µg or 50 µg of Meningococcal Serogroup B Outer Membrane Vesicle Vaccine MenPF-1

Conditions
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Serogroup B Meningococcal Disease

Intervention / Treatment
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* Biological: MenPF-1.


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Willing and able to give informed consent for participation in the study Aged between 18 and 50 years In good health as determined by medical history, physical examination and clinical judgment of the investigators (Females) Willing to use effective contraception (such as the oral contraceptive pill, contraceptive implant or barrier methods) from one month prior and for the duration of the study Able to attend the scheduled visits and to comply with all study procedures, including internet access for the recording of diary cards Willing to allow his or her General Practitioner and/or Consultant, if appropriate, to be notified of participation in the study Confirmation from GP that they are aware of the inclusion and exclusion criteria and are satisfied from their knowledge of the volunteer that they are suitable to enrol Exclusion Criteria: History of significant organ/system disease that could interfere with trial conduct or completion Have any known or suspected impairment or alteration of immune function Study significant abnormalities on screening investigations at the discretion of an Investigator Receipt of a live vaccine within 4 weeks prior to vaccination or a killed vaccine within 7 days prior to vaccination Plan to receive any vaccine other than the study vaccine within 4 weeks following vaccination Scheduled procedures requiring general anaesthesia during the study Participant who is terminally ill Receipt of immunoglobulin or any blood product transfusion within 3 months of study start Participation in another research study involving an investigational product in the past 12 weeks, or are planning to do so within the 20 weeks of this study Previously having received a meningococcal B vaccine of any kind Previous occurrence of disease caused by N. meningitidis Inability, in the opinion of the Investigator, to comply with all study requirements Female participants who are pregnant, lactating or planning pregnancy during the course of the study Any other significant disease or disorder which, in the opinion of the Investigator, may Put the participants at risk because of participation in the study Influence the result of the study Impair the participant's ability to participate in the study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Low dose vaccine arm<br>To investigate safety, tolerability and immunogenicity of 25 µg of the serogroup B meningococcal protein vaccine MenPF-1 in healthy adults aged 18 to 50 years of age when given three doses of vaccine with 8 weeks interval. | Biological: MenPF-1.<br>* A new vaccine against meningococcal disease.<br>|
| Experimental: High dose vaccine arm<br>To investigate safety, tolerability and immunogenicity of 50 µg of the serogroup B meningococcal protein vaccine MenPF-1 in healthy adults aged 18 to 50 years of age when given three doses of vaccine with 8 weeks interval. | Biological: MenPF-1.<br>* A new vaccine against meningococcal disease.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To investigate safety and tolerability of 25 µg or 50 µg of the serogroup B meningococcal protein vaccine MenPF-1 | This will be measured by the recording and assessment of the following local and systemic adverse events following administration of each vaccine dose: Tenderness and pain at the injection site Induration Redness Swelling Headache Malaise Myalgia Nausea and/or vomiting Fever Blood parameters Any unsolicited symptom(s) not listed above | 20 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Immunogenicity | Immunological assays to study the immune responses to vaccines, including: Serum bactericidal antibody (SBA) assay. Antibody concentration against vaccine antigens. Quantification of circulating vaccine-induced B-cells. Quantification of vaccine-induced, antigen specific T-cell responses and associated cytokine production. Serum opsonophagocytic activity. Gene expression profile after immunization and DNA storage for investigation of the genetic associations with the immune response. | 20 weeks |