Influence of Early Menopause on Sympathetic Activation and Cardiovascular Function in Older Women

Study Overview
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Brief Summary
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Women who enter menopause early are at a greater risk for developing cardiovascular disease later in life as compared to women with normal onset of menopause. This increased risk may be due to a prolonged length of time with decreased hormone levels post-menopause; however, this health risk remains understudied. The current study plan to study why women with early menopause are at higher risk for cardiovascular disease by evaluating their sympathetic nervous system and heart-blood vessel function. In addition, there is lack of promising treatment plans for cardiovascular disease in post-menopausal women. Therefore, the current study will also test the impact of dietary nitrate on post-menopausal women to determine if it might serve as a potential treatment to reduce risk of cardiovascular disease in older women.

Detailed Description
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The current study is recruiting two groups of women aged between 60-70 years old; 1) women who had early natural/non-surgical menopause (age 40-44 yrs) and 2) women who had normal natural/non-surgical menopause (age 50-54). The investigators are recruiting women who do not have any cardiovascular disease, hypertension, diabetes, and other major health issue. The main hypothesis of the project is that early menopause negatively impacts the heart, blood vessel and nerve system due to the increased length of time with reduced hormone levels; which could explain the relationship between early menopause and greater risk of cardiovascular disease in later life. Sympathetic nerve system and cardiovascular functions will be evaluated in both groups to compare the impact of prolonged hormone deficiency. In addition, current study will examine the impact of inorganic nitrate supplementation (beet root juice) to see if there are any favorable changes after 2 weeks of beet root juice intake. There are 2 main test visits in the current study. Visit 1. Nerve system test (maximally take about 5 hours): Participants will rest in the supine position for ≥ 20 mins. Resting heart rate, breathing rate, finger blood pressure will be monitored. Cardiac output will be assessed using the acetylene gas rebreathing technique (1 hour). IV will be inserted for blood draw. Nerve signal search will be performed using a thin needle behind knee to find a signal from a specific nerve. At the same time, transcranial Doppler probe will be placed on the head to look at brain blood flow using soft head gear (1-2 hour). After acceptable signals are obtained, baseline data collection will begin. It will be followed by handgrip exercise and hand immersion to cold water to examine neural sympathetic response to different stimuli (30 mins). After this, to evaluate nerve response to different degrees of passive standings, a bed will be tilted to 30° and 60° for 8 mins each. Blood samples will be obtained in the supine resting position and at the end of 60° tilt (45 mins). Visit 2. Heart and vessel function assessment (Proximately take 2 hours): All measurements are non-invasive for this visit. Participants will rest in the supine position for ≥ 20 mins. Resting heart function will be assessed by ultrasound echocardiography (30mins). Vascular stiffness measurement will be performed using a probe placed on wrist, neck and leg groin area (30 mins). Vascular endothelial function will be measured using flow-mediated dilation and followed by smooth muscle responsiveness test will be performed by measuring brachial artery diameter changes for 10 min following 0.4 mg sublingual nitroglycerin administration (1 hour). After Visit 1-2, participant will be asked to drink either dietary nitrate supplementation or placebo for 2 weeks (randomly assigned). After 2 weeks intervention, participant will come back for post-intervention tests which are identical to visit 1 and 2. After completion of the first intervention, participants will have at least 2 weeks of wash-out period. After 2 weeks participant will be back to the lab and repeat the visit 1 and 2, as well as 2 weeks of dietary nitrate supplementation or placebo. (For those who received 2 weeks of nitrate supplementation for the first intervention, it will be a placebo for this intervention. For those who received placebo for the first intervention, it will be a nitrate supplementation for this intervention). After intervention participants will repeat the visit 1 and 2 again.

Official Title
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Influence of Early Menopause on Sympathetic Activation and Cardiovascular Function in Older Women

Conditions
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Early Menopause, Menopause

Intervention / Treatment
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* Dietary Supplement: Nitrate supplementation
* Dietary Supplement: placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy normotensive postmenopausal women Current age between 60 and 70 years Who have experienced natural or non-surgical menopause Exclusion Criteria: Any evidence of cardiopulmonary disease History of hormone replacement therapy in the last 5 years Chronic kidney disease Diabetes mellitus Hypertension Current abuse of alcohol or drugs and use of tobacco products Surgical procedure induced menopause

Ages Eligible for Study
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Minimum Age: 60 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: 2 groups of women (early and normal menopause transition) will be enrolled to crossover design study in a fashion of randomized and double-blinded.
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Nitrate supplementation<br>Nitrate rich (6.8 mmol) beet root juice (70ml) twice a day | Dietary Supplement: Nitrate supplementation<br>* Subjects will drink 70 ml of beet root juice twice a day for 2 weeks.<br>|
| Placebo Comparator: Placebo<br>Nitrate depleted (0.04 mmol) placebo juice (70ml) twice a day | Dietary Supplement: placebo<br>* Subjects will drink 70 ml of nitrate depleted placebo juice twice a day for 2 weeks.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle sympathetic nerve activity (MSNA) | Muscle sympathetic nerve activity will be measured using microneurography at the peroneal nerve. | Change from baseline resting MSNA to 2 weeks post nitrate supplementation resting MSNA | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Cardiac Echocardiogram | Systolic and diastolic cardiac function will be measured using high resolution ultrasound echocardiogram | Change from baseline cardiac function to 2 weeks post nitrate supplementation resting cardiac function | 
| Vascular endothelial function | Brachial artery endothelial function will be measured using flow-mediated dilation | Change from baseline brachial artery endothelial function to 2 weeks post nitrate supplementation brachial artery endothelial function | 
| Arterial stiffness/wave properties | Central and peripheral pulse wave velocity and wave reflection will be assessed using arterial tonometry | Change from baseline pulse wave velocity and wave reflection to 2 weeks post nitrate supplementation resting pulse wave velocity and wave reflection | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Neural control, Cardiovascular function, Inorganic nitrate supplementation