Vitamin D and Disease Activity in Ulcerative Colitis

Study Overview
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Brief Summary
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The purpose of this study is to test blood and tissue samples of people with Ulcerative Colitis (UC) to see what effects vitamin D may have on the immune system. This research is being done because it could lead to the development of new treatment for people with inflammatory Bowel Disease (IBD).

Detailed Description
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Patients with UC will be recruited and consented prior to colonoscopy or flexible sigmoidoscopy. All patients will be age 18 or older with a known history of UC that has been confirmed by biopsy and an extent of disease of at least 20cm proximal to the anus. Clinical data will be collected on the patient's age, disease duration, family history of IBD, smoking status and history, as well as current medications for IBD through a questionnaire. Data will also be collected regarding current vitamin D supplementation (if taking) and dose. Prior to the endoscopic procedure, peripheral blood will be drawn into two 10ml red top tubes. At the time of endoscopy, five standard size mucosal pinch biopsies will be obtained from 20cm proximal to the anus. . A bar-coded unique identifier label will be applied to each sample. One biopsy will be placed in formalin. Paraffin samples will be maintained at room temperature. Following to the endoscopic procedure, the patient's physician will complete a Mayo score based on the patients current symptoms and endoscopic disease severity. Comparison will be made between disease activity, serum serum 25 hydroxyvitamin D level, and mucosal expression of tight junction proteins as well as proinflammatory cytokines.

Official Title
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Association Between Vitamin D Level and Disease Activity in Ulcerative Colitis

Conditions
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Ulcerative Colitis

Intervention / Treatment
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* Other: No intervention


Participation Criteria
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Eligibility Criteria
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Inclusion: Age 18 years old and over A diagnosis of ulcerative colitis that has been confirmed by biopsy and an extent of disease of at least 20cm proximal to the anus. Exclusion: Age <18 years old Diagnosis of Crohn's disease Diagnosis of ulcerative colitis with disease extent <20cm proximal to the anus

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: No intervention|This study is not interventional|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 25(OH) Vitamin D level (ng/ml) |  | 1 day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Mayo Endoscopy Score |  | 1 day | 
| mucosal zo-1 expression by real time PCR |  | 1 day | 
| mucosal occludin expression by real time PCR |  | 1 day | 
| mucosal VDR expression by real time PCR |  | 1 day | 
| mucosal claudin-2 expression by real time PCR |  | 1 day | 
| mucosal TNFa expression by real time PCR |  | 1 day | 
| mucosal IL-8 expression by real time PCR |  | 1 day | 
| mucosal VDR expression by immunohistochemistry |  | 1 day | 
| mucosal zo-1 expression by immunohistochemistry |  | 1 day | 
| mucosal occludin expression by immunohistochemistry |  | 1 day | 
| mucosal claudin-2 by immunohistochemistry |  | 1 day |