A Study of Lung Clearance After Hypertonic Saline Delivery Using the tPAD Device

Study Overview
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Brief Summary
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The primary objective of this study is to determine whether a single overnight, eight-hour administration of a 7% NaCl solution delivered by the Parion Sciences transnasal Pulmonary Aerosol Delivery (tPAD) device has a significant effect on mucociliary clearance in subjects with cystic fibrosis, as compared to no treatment. This study will be conducted at the University of Pittsburgh Medical Center.

Detailed Description
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Cystic Fibrosis (CF) lung disease is caused by dehydration of airway secretions that leads to mucus adhesion, infection and airways inflammation. A simple means to restore hydration to CF airway surfaces is to inhale hypertonic (3-7% NaCl) saline, which osmotically draws water onto the airway surface. Rehydration of the lubricant layer of the airway surface liquid facilitates mucociliary clearance (MCC) and therefore the removal of inhaled infectious agents. Recent studies have described (1) the short term (two weeks) beneficial effects of inhaled hypertonic saline (HS) four times daily on pulmonary function, MCC, and quality of life and (2) the long term (one year) benefits of inhaled HS twice daily on lung function and pulmonary exacerbation frequency. Consequently, inhaled HS is now used by  55% of patients with CF nationwide. Due to the large number of medications that CF patients use on a daily basis in conjunction with airway clearance techniques, there is a high treatment burden that results in decreased quality of life. Both the Cystic Fibrosis Foundation and leading CF clinicians support the idea that the use of hypertonic saline is now a standard of care. The investigators believe the use of a specialized transnasal Pulmonary Aerosol Delivery (tPAD) device for administration of HS will improve on that standard of care by reducing the treatment burden during CF patients' waking hours, ensuring greater compliance and potentially improving the efficacy and tolerability of inhaled HS. A previous deposition study with the tPAD, in six healthy adult subjects, demonstrated  38% of the 7% HS aerosol emitted from the nasal cannula is deposited in the adult lungs, with no acute safety or tolerability issues (Parion Sciences Protocol PS-D100-102; Scott Donaldson, PI). This deposition efficiency matched that of the Pari LC Star used via the oral route, which was used as a standard of practice comparator. However, 7% HS nebulization by the tPAD resulted in a more peripheral deposition of the aerosol than the Pari LC Star comparator. Previously, it has been shown that administration of 5 mL of 7% HS QID by the Pari LC Star leads to a significant improvement in the lung function in CF patients. The investigators estimate that this dosing regimen deposits  400 mg of NaCl per day but requires four  18 minute administrations (deposition rate = 5.8 mg/min). Although HS is generally well tolerated in the CF population, intolerance does occur and is largely related to the rate of NaCl delivery to the oropharynx and airway surfaces. As nebulizer devices capable of administering aerosols through a nasal cannula are not currently available, Parion Sciences has designed a customized nebulizer spacer that entrains the aerosol from an approved and marketed Aerogen Aeroneb Pro vibrating mesh nebulizer into a nasal cannula without significant rain out or dripping from the cannula. The tPAD system being utilized has an output rate of  50 ul/min, and so emits  3.5 mg/min of NaCl and deposits  1.3 mg/min in the lung (38% deposition efficiency). If used overnight for 8 hours, the investigators estimate that 640 mg NaCl will be deposited in the lung. Therefore, the investigators project that this novel administration system has the capacity to deliver approximately 50% greater mass of NaCl to the lung when used overnight, compared to 4 times a day treatment with a standard oral nebulizer, thereby potentially increasing efficacy. However, because the lung deposition rate is less than 25% that of the standard oral nebulizer, the investigators anticipate that the tPAD will also be better tolerated and will eliminate the need for daytime HS treatments. In this study, the investigators will explore the safety, tolerability and effect on mucociliary and absorptive clearance rates of a 7% HS solution administered continuously overnight via the transnasal route. The tPAD is a non-significant risk device which is composed of a 510K approved Aerogen Aeroneb Pro vibrating mesh nebulizer with a custom nebulization chamber that allows for connection of a standard nasal cannula. Protocol PS-D201 is funded by NIH Grant 2R44HL110502-02 Hypertonic Saline for Cystic Fibrosis.

Official Title
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A Randomized Crossover Mucociliary Clearance Study of Aerosolized 7% NaCl Solution Administered Overnight by the tPAD Device to Subjects With Cystic Fibrosis

Conditions
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Cystic Fibrosis

Intervention / Treatment
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* Drug: inhaled hypertonic saline (7%)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Has a diagnosis of CF via standard criteria Is aged 18 years or older Is capable of providing written informed consent in English to participate in the study. Has a forced expiratory volume in 1 second (FEV1) >= 40% and < 110% predicted normal for age, gender, and height at Screening. Has a body mass index (BMI) < 30 kg/m2 Can tolerate cessation of treatment with HS for 72 hours and rhDNase for 24 hours prior to each treatment visit until discharge from Visits 2 and 3. Can tolerate cessation of treatment with long-acting beta-agonists (LABAs) for 12 hours and short-acting beta-agonists (SABAs) for 6 hours prior to radioaerosol administration for each MCC measurement and at least until discharge from Visits 2 and 3 Is on a stable medication regimen for at least 28 days before start of dosing and can continue such regimen for duration of study Tolerates the 30 minute administration of 7% HS by the tPAD device at screening without subjective intolerance, oxyhemoglobin desaturation, or significant change in spirometry (>10% reduction from pre-dose value in FEV1, measured 30 minutes after completion of the aerosol administration) Exclusion Criteria: Has evidence of an acute upper or lower respiratory infection or clinically significant illness at screening or within 28 days prior to the start of dosing Required an acute intervention with antibiotics (oral, inhaled, or IV) or systemic corticosteroids within the last 28 days for a respiratory illness Has a history of intolerance to a beta-agonist or hypertonic saline Has evidence of significant nasal obstruction that impairs the ability to breathe through the nose Has a clinical diagnosis of sleep apnea Has current symptoms of allergic rhinitis Is unable to maintain a stable dosage regimen of any concomitant medication throughout the duration of the trial. Has participated in a clinical drug or investigational device trial within the past 28 days Has a history of positive test for Burkholderia cepacia Has a present history of any clinically significant and uncontrolled neurologic, gastrointestinal, renal, hepatic, cardiovascular (including hyper/hypotension and tachy/bradycardia), psychological, pulmonary, metabolic, endocrine, or hematological disorder or disease, or any other major disorder or disease, in the opinion of the investigator Has a history of smoking within the last 12 months Is known to be pregnant, has a positive urine pregnancy test or is nursing (female subjects only) Should not participate in the study, in the opinion of the Principal or Clinical investigator

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: HS/sham<br>Subjects will utilize inhaled hypertonic saline (7%) delivered using the tPAD device during one session and perform a sham treatment with the tPAD during the other. The order will be randomized. | Drug: inhaled hypertonic saline (7%)<br>* Inhaled hypertonic saline delivered by nasal cannula using the Parion transnasal Pulmonary Aerosol Delivery (tPAD) device<br>* Other names: PulmoSal 7% (pH+);|
| Experimental: sham/HS<br>Subjects will utilize inhaled hypertonic saline (7%) delivered using the tPAD device during one session and perform a sham treatment with the tPAD during the other. The order will be randomized. | Drug: inhaled hypertonic saline (7%)<br>* Inhaled hypertonic saline delivered by nasal cannula using the Parion transnasal Pulmonary Aerosol Delivery (tPAD) device<br>* Other names: PulmoSal 7% (pH+);|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| mucociliary clearance | Mucociliary clearance as assessed through the imaging of radiolabeled particles | 6 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| safety and tolerability measurements | clinical adverse events assessment | 12 hours | 
| pulmonary function measurements | One-second forced expiratory volume (FEV1) and forced vital capacity (FVC) will be measured through breathing tests at the beginning and end of both testing visits. | 12 hours | 
| safety and tolerability measurements | Sleep tolerability questionnaire | 12 hours | 
| safety and tolerability measurements | Sino-nasal symptoms questionnaire | 12 hours | 
| safety and tolerability measurements | Assessment Of Device Experience | 12 hours | 
| DTPA absorption | Measurement of the absorptive clearance of Indium111-DTPA from the airways | 6 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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cystic fibrosis, hypertonic saline, nebulizer, nasal cannula