Sargramostim Following Allogeneic Bone Marrow Transplantation in Treating Patients With Chronic Myelogenous Leukemia

Study Overview
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Brief Summary
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RATIONALE: Drugs used in chemotherapy work in different ways to stop cancer cells from dividing so they stop growing or die. Combining chemotherapy with allogeneic bone marrow transplantation may allow the doctor to give higher doses of chemotherapy drugs and kill more cancer cells. Colony-stimulating factors such as sargramostim may increase the number of immune cells found in bone marrow or peripheral blood, and may help a person's immune system recover from the side effects of chemotherapy. PURPOSE: Phase II trial to study the effectiveness of allogeneic bone marrow transplantation followed by sargramostim in treating patients who have chronic myelogenous leukemia.

Detailed Description
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OBJECTIVES: Determine whether the use of sargramostim (GM-CSF) after T-cell depleted, CD34-positive cell-supplemented allogeneic bone marrow transplantation can reduce leukemic relapse in patients with chronic myelogenous leukemia. OUTLINE: Patients receive myeloablation with busulfan and cyclophosphamide on an approved protocol. Allogeneic bone marrow is harvested and treated in vitro with anti-CD34 antibody. T-cell depleted, CD34-positive cell-supplemented bone marrow is infused on day 0. Patients receive high-dose sargramostim (GM-CSF) subcutaneously (SC) beginning on day 5 and continuing until blood counts recover and then low-dose GM-CSF SC continuing until day 60. Donor lymphocyte infusions or second unmodified allogeneic bone marrow transplantation without GM-CSF is considered in case of primary or secondary engraftment failure. Patients are followed every month for 3 months, every 3 months for 1 year, every 6 months for 1 year, and then annually thereafter. PROJECTED ACCRUAL: A total of 40 patients will be accrued for this study within approximately 6-10 years.

Official Title
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GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR (Rhu-GM-CSF) FOR REDUCTION OF LEUKEMIC RELAPSE AFTER T-LYMPHOCYTE DEPLETED ALLOGENEIC BMT FOR CHRONIC MYELOID LEUKEMIA

Conditions
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Leukemia

Intervention / Treatment
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* Biological: sargramostim
* Biological: therapeutic allogeneic lymphocytes
* Procedure: allogeneic bone marrow transplantation
* Procedure: in vitro-treated bone marrow transplantation


Participation Criteria
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Eligibility Criteria
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DISEASE CHARACTERISTICS: Diagnosis of chronic myelogenous leukemia (CML) documented by cytogenetic and molecular analyses at Johns Hopkins Philadelphia chromosome (Ph)-positive or -negative CML Ph-negative CML allowed with presence of either: BCR-ABL rearrangement (on molecular, fluorescent in situ hybridization, or polymerase chain reaction analyses) p210 protein One of the following: Patient age 18 to 65 Disease duration longer than 3 years Accelerated phase CML Accelerated phase diagnosis based on any of the following: More than 10% to less than 30% blasts in blood or bone marrow No hematologic response to prior conventional therapy (hydroxyurea or interferon) Extramedullary disease (e.g., progressive splenomegaly or lymphadenopathy) Basophilia greater than 10% in blood or bone marrow Other cytogenetic abnormalities in addition to a single Ph chromosome Second chronic phase Failure on interferon suggested of patients over age 18 with chronic phase CML, with failure defined as: No detectable Ph-negative metaphases in marrow after 6 months No progressive increase in Ph-negative metaphases in marrow after 6-12 months Less than 50% Ph-negative metaphases after 1 year No complete cytogenetic remission after 2 years Intolerance to interferon therapy No blast crisis CML, chronic myelomonocytic leukemia, or juvenile CML The following conditions are allowed: Leukocyte count abnormalities Fibrosis Anemia Fever or bone pain Thrombocytopenia Bone marrow reticulin Availability of an HLA-identical sibling donor At least 3 years of age (priority given to donors over age 10) Priority given to CMV-negative donor if patient CMV-negative No medical or psychiatric condition that precludes transplant procedure PATIENT CHARACTERISTICS: Age 18 to 65 Performance status ECOG 0-1 Life expectancy Not specified Hematopoietic See Disease Characteristics Hepatic Not specified Renal Not specified Other No history of intolerance to sargramostim (GM-CSF) PRIOR CONCURRENT THERAPY: Biologic therapy See Disease Characteristics Chemotherapy See Disease Characteristics Endocrine therapy Not specified Radiotherapy Not specified Surgery Not specified

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Masking: None (Open Label)


Arms and Interventions

| Intervention/Treatment |
| --- |
|Biological: sargramostim|nan|
|Biological: therapeutic allogeneic lymphocytes|nan|
|Procedure: allogeneic bone marrow transplantation|nan|
|Procedure: in vitro-treated bone marrow transplantation|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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relapsing chronic myelogenous leukemia, chronic phase chronic myelogenous leukemia, accelerated phase chronic myelogenous leukemia, Philadelphia chromosome positive chronic myelogenous leukemia, Philadelphia chromosome negative chronic myelogenous leukemia, atypical chronic myeloid leukemia