Serum Kisspeptin Levels in Infertile Women

Study Overview
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Brief Summary
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The study population is comprise of 90 women those age varies between 18-38 year-old. The first group will comprise of 30 women with polycystic ovaries or anovulatory cycles, the second group will comprise of 30 women with diagnosis of unexplained infertility and the third group will comprise of 30 women those partners with male subfertility. A 2 cc blood sample for Kisspeptin analysis will be drawn from the antecubital vein from each participants. The patients will receive 50 mg clomiphene citrate at the fifth day of the menstrual period and follicular development will be measured with serial ultrasound follow up.

Detailed Description
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The study population is comprise of 90 women those age varies between 18-38 year-old with wish of baby for at least one year despite of unprotected sexual intercourse, patent tubes confirmed in hysterosalpingography , follicle stimulating hormone levels <12 IU. The first group will comprise of 30 women with polycystic ovaries in ultrasound examination or anovulatory cycles, the second group will comprise of 30 women with diagnosis of unexplained infertility, normal menstrual periods and without known male factor infertility and the third group will comprise of 30 women those partners with sperm count between 5x106 -15x106 /mL, type A + type B motility <%32 and Kruger morphology <4%. A 2 cc blood sample for Kisspeptin analysis will be drawn from the antecubital vein from each participants and centrifuged and sera will be kept in -80 C till recruitment has been completed. Kisspeptin, E2, Progesterone, anti-mullerian hormone levels will be measured with ELISA method. The patients will receive 50 mg clomiphene citrate at the fifth day of the menstrual period and follicular development will be measured with serial ultrasound follow up.

Official Title
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Serum Kisspeptin Levels in Infertile Women Due to Anovulatory Cycles, Unexplained Infertility or Male Subfertility: A Prospective Observational Study

Conditions
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Infertility, Female, Unexplained Infertility, Anovulation

Intervention / Treatment
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* Other: Blood sample


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: ages varying between 18-38 year-old with wish of baby for at least one year despite of unprotected sexual intercourse patent tubes confirmed in hysterosalpingography follicle stimulating hormone levels <12 IU no other relevant medical history Exclusion Criteria: ages other than 18-38 year-old with wish of baby for least than one year despite of unprotected sexual intercourse obstructed tubes confirmed in hysterosalpingography follicle stimulating hormone levels >12 IU history thyroid disease increased prolactin levels history of chemo/radiation therapy

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 38 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group 1<br>The first group will comprise of 30 women with polycystic ovaries in ultrasound examination or anovulatory cycles. | Other: Blood sample<br>* Serum analysis<br>* Other names: Serum Kisspeptin levels;|
| Group 2<br>The second group will comprise of 30 women with diagnosis of unexplained infertility, normal menstrual periods and without known male factor infertility. | Other: Blood sample<br>* Serum analysis<br>* Other names: Serum Kisspeptin levels;|
| Group 3<br>The third group will comprise of 30 women those partners with sperm count between 5x106 -15x106 /mL, type A + type B motility <%32 and Kruger morphology <4%. | Other: Blood sample<br>* Serum analysis<br>* Other names: Serum Kisspeptin levels;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The relation of Kisspeptin levels and female infertility |  | 1 year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The relation of Kisspeptin levels and follicular growth |  | 1 year |