Influence of Dietary Fiber-rich Meals on Gene Expression and Postprandial Glucose and Lipid Response

Study Overview
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Brief Summary
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The aim of this study is to Measure the effect on gene expression in leukocytes from a meal rich in oat bran Investigate the postprandial glucose, insulin and triglyceride responses after intake of meals containing fiber from different sources (oat, rye and sugar beet fiber) or a meal containing a mixture of these three fibers

Detailed Description
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Dietary fiber has long been known to give beneficial health effects. Yet, the understanding of how fiber-rich meals regulate molecular events at a gene level is limited. Also, few studies have compared the effects of different fiber sources on postprandial responses and hardly any study the effects of fiber mixtures in the same meal, even though this is more similar to regular eating habits. Healthy subjects will come to the study center after an overnight fast, to ingest breakfasts randomly enriched with different fiber. The meals contains either spray-dried oat drink, rye bran, sugar beet fiber a mixture of these three fibers, oat bran or no added fiber (control). All meals are adjusted to contain the same total amount of available carbohydrates and fat. Blood leukocytes for gene expression profiling were taken before and 2 h after consumption while blood samples for analysis of postprandial glucose, insulin and triglyceride levels were taken every 30 min during 3 h. NuGO Affymetrix Human Genechip NuGO_Hs1a520180 are used for the microarray analysis and analysis is performed with linear mixed models and enrichment analysis to identify functional gene sets that responded to the specific oat bran effect.

Official Title
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The Influence of Dietary Fibre-rich Meals on Gene Expression in Leukocytes and Postprandial Glucose and Lipid Response in Healthy Subjects

Conditions
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Hypoglycemia, Hyperglycemia

Intervention / Treatment
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* Other: Oat bran
* Other: Spray-dried oat drink
* Other: Rye bran
* Other: Sugar beet fiber
* Other: Mixture of fiber
* Other: Control


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI 18-30 Exclusion Criteria: pregnancy breastfeeding diabetes mellitus hepatitis B blood lipid lowering pharmaceuticals intolerance or allergy to cereals or sugar beet fiber

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Factorial Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Mixture of fiber<br>Single intake of a mixture of spray-dried oat drink, rye bran and sugar beet fiber | Other: Mixture of fiber<br>* 38 g spray-dried oat drink, 30 g rye bran and 6 g sugar beet fiber were added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (18 g total fiber).<br>|
| Experimental: Sugar beet fiber<br>Single intake of sugar beet fiber | Other: Sugar beet fiber<br>* 19 g sugar beet fiber was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12 g total fiber).<br>* Other names: Pectin;|
| Experimental: Rye bran<br>Single intake of rye bran | Other: Rye bran<br>* 31 g rye bran was added to 250 ml blackcurrant beverage with pulp to give 1.7 g soluble fiber (12 g total fiber).<br>* Other names: Arabinoxylan;|
| Experimental: Oat bran<br>Single intake of oat bran | Other: Oat bran<br>* 82 g oat bran was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12.6 g total fiber).<br>* Other names: Beta-glucan;|
| Experimental: Spray-dried oat drink<br>Single intake of spray-dried oat drink | Other: Spray-dried oat drink<br>* 62 g spray-dried oat drink was added to 250 ml blackcurrant beverage with pulp to give 2.7 g soluble fiber (3.3 g total fiber).<br>* Other names: Beta-glucan;|
| Placebo Comparator: Control<br>Single intake of a meal with no added fiber | Other: Control<br>* No fiber was added to control meal (250 g black-currant beverage)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changed gene expression profile by fiber-rich meals |  | 2 h after meal intake | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Lowering of postprandial glucose by fiber-rich meals |  | 0-180 min after meal intake | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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gene expression, oats, rye, sugar beet fiber, glucose, insulin, triglyceride, decreased postprandial glucose, decreased postprandial insulin, glycemic index