Intestinal Microbiota and Postoperative Ileus After Colorectal Surgery

Study Overview
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Brief Summary
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Postoperative ileus (POI) after colorectal surgery is frequent and is a burden for national health authority because it increases the morbidity and the length of hospital stay. Some of the stage of the mechanism of POI are known and are now targeted to reduce its occurence but despite these measures, POI still happens in 10-30% of surgeries. The role of the intestinal microbiota in POI is unknown while it could be a new target to reduce its duration or its occurence.

Official Title
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Evaluation of the Role of the Intestinal Microbiota in the Mechanism of Postoperative Ileum After Colorectal Surgery : a Preliminary Study

Conditions
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Postoperative Ileus, Surgery-Complications

Intervention / Treatment
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* Other: Feces collection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: laparoscopic colorectal resection with anastomosis and without diverting stoma Exclusion Criteria: risk of modification of the microbiota (terminal enterostomy, chronic inflammatory bowel disease, oesophageal or gastric surgery, vagal nerves lesion, beta lactamin allergy, bowel cleansing) postoperative complication

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group Ileus<br>Patients that experience POI after colorectal surgery. POI is defined by the presence of 2 of the 5 criteria of Vather from the first postoperative day. The first 10 consecutive patients experiencing POI will be included in the study. | Other: Feces collection<br>* Feces will be collected, at home, by the patient., by using a collection kit. The stools are collected in the 2 last days before surgery. After surgery, the first stool is collected, during the hospitalization, by using the same kit. Stools are conserved in the same condition, before being frozen in our local biologic ressources center.<br>|
| No POI group<br>Patients that do not experience POI. The 10 patients having the fastest recovery of gastro-intestinal functions after surgery during the period of inclusion will be included in the study | Other: Feces collection<br>* Feces will be collected, at home, by the patient., by using a collection kit. The stools are collected in the 2 last days before surgery. After surgery, the first stool is collected, during the hospitalization, by using the same kit. Stools are conserved in the same condition, before being frozen in our local biologic ressources center.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Composition of the feces microbiota before surgery | The composition of the microbiota will be assessed by the analysis of the region V3 V4 of the 16S rRNA, amplificateur by high flow sequencing | within the 2 last days before surgery | 
| Composition of the feces microbiota after surgery | The composition of the microbiota will be assessed by the analysis of the region | up to the first postoperative stools, an average of 3 postoperative days |