Exploratory Study of Natural Killer Cells in Human Skin

Study Overview
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Brief Summary
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Natural Killer (NK) cells play a unique role during innate immune responses as they are able to recognize and eliminate, without specific sensitization, tumors, microbe-infected cells as well as allogeneic cells.In a first time, we will characterize the tissue distribution, the phenotype and the effector functions of NK cells present in the human healthy skin.

Detailed Description
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Then, we will define whether these parameters are qualitatively/ quantitatively modified in the following skin pathologies: a) atopic dermatitis; b) contact dermatitis; c) psoriasis ; d) lichen planus ; e) GVH (graft versus host disease) ; (f) melanoma.

Official Title
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Exploratory Study of Natural Killer Cells in Human Skin

Conditions
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Atopic Dermatitis, Contact Dermatitis, Psoriasis, Lichen Planus, Graft Versus Host Disease, Melanoma

Intervention / Treatment
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* Other: Biopsy and sample of blood
* Other: Biopsy and sample of blood
* Other: Biopsy and sample of blood
* Other: Biopsy and sample of blood
* Other: Biopsy and sample of blood
* Other: Biopsy and sample of blood
* Other: Biopsy and sample of blood


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age ≥ 18 years. Consent obtained after healthy donor or patient information. Patients suffering from one of the following diseases: a) atopic dermatitis ; b) contact dermatitis; c) psoriasis ; d) lichen planus ; e) GVH ; f) melanoma. Exclusion Criteria: Age below 18, pregnant or breastfeeding women, persons under guardianship, persons without social security, prisoners, persons hospitalized without consent on the basis of French laws L. 3212-1 et L. 3213-1. Patients affected by HIV infection, AIDS, Hepatitis A, B or C infection. Neutropenia <1000/mm3. Thrombopenia <50 000/mm3.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: 1<br>Healthy volunteer | Other: Biopsy and sample of blood<br>* Biopsy and sample of blood<br>|
| Other: 2<br>atopic dermatitis patient | Other: Biopsy and sample of blood<br>* Biopsy and sample of blood<br>|
| Other: 3<br>contact dermatitis patient | Other: Biopsy and sample of blood<br>* Biopsy and sample of blood<br>|
| Other: 4<br>psoriasis patient | Other: Biopsy and sample of blood<br>* Biopsy and sample of blood<br>|
| Other: 5<br>lichen planus patient | Other: Biopsy and sample of blood<br>* Biopsy and sample of blood<br>|
| Other: 6<br>GVH patient | Other: Biopsy and sample of blood<br>* Biopsy and sample of blood<br>|
| Other: 7<br>melanoma patient | Other: Biopsy and sample of blood<br>* Biopsy and sample of blood<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| In a first time, we will characterize the tissue distribution, the phenotype and the effector functions of NK cells present in the human healthy skin. |  | 12 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To determine whether the eventual modifications detected in skin pathologies are correlated to qualitative/ quantitative alterations of NK cell subsets present in the peripheral blood. |  | 12 months |