Acute Exercise and Microvascular Function

Study Overview
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Brief Summary
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It is well recognized that chronic exercise protects against the development of cardiovascular diseases. However, the responses of vasculature to acute exercise (AE) are not well known and not consistent in literature. Acute exertional exercise can induce large, transient increases in arterial pressure and development of metabolic acidosis which can be associated with pro-inflammatory response involving oxidant stress and circulating cytokines known to impair endothelial function. The aim of this study is to test the impact of a single exposure to exhausting training on microvascular reactivity in healthy sedentary subjects and athletes.

Official Title
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Influence of Acute Exhausting Exercise on Microvascular Reactivity in Healthy Sedentary Subjects and Athletes

Conditions
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Physical Exertion

Intervention / Treatment
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* Other: Acute Exhausting Exercise (AE)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy volunteers active athletes lean sedentary subjects Exclusion Criteria: drugs that could affect the endothelium overweight hypertension coronary artery disease diabetes hyperlipidaemia renal impairment cerebrovascular and peripheral artery disease

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Sedentary Subjects<br>Healthy lean sedentary subjects. | Other: Acute Exhausting Exercise (AE)<br>* A single progressive rowing training to maximal exhaustion (AE).<br>|
| Experimental: Athletes<br>Active athletes. | Other: Acute Exhausting Exercise (AE)<br>* A single progressive rowing training to maximal exhaustion (AE).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Skin microcirculation post occlusive reactive hyperemia | Cutaneous microvascular blood flow will be measured by Laser Doppler Flowmetry in response to vascular occlusion (post occlusive reactive hyperemia - PORH) before and after acute exhausting training. | Post occlusive reactive hyperemia - PORH, recorded by Laser Doppler Flowmetry will be measured immediately after acute exhausting training. | 
| Skin microcirculation acetylcholine induced dilation | Cutaneous microvascular blood flow will be measured by Laser Doppler Flowmetry in response to iontophoresis of acetylcholine (acetylcholine induced dilation, AChID) before and after acute exhausting training. | Acetylcholine induced dilation (AChID), recorded by Laser Doppler Flowmetry will be measured immediately after acute exhausting training. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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microvascular function, laser Doppler flowmetry, exercise