Effects of Low-density Lipoprotein (LDL) Apheresis on Inflammatory and Lipid Markers

Study Overview
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Brief Summary
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The primary objective of this study is to measure how LDL apheresis affects levels of inflammatory and cholesterol markers in human beings. The investigators will address this question by drawing pre- and post-LDL apheresis blood from patients who are undergoing this procedure. A secondary objective of this study is to learn how specific inflammatory markers behave in our blood in terms of time to rebound back to normal levels. The investigators will address this question by drawing post-LDL apheresis blood at predetermined time intervals.

Detailed Description
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Numerous epidemiological investigations have demonstrated the importance of cholesterol - specifically low density lipoprotein (LDL) - in the development and progression of atherosclerosis. A continuing relationship between cholesterol level and coronary morbidity has been established. The initial approach for managing elevated cholesterol includes lifestyle interventions, namely eating a low fat diet, weight loss in overweight patients, and regular aerobic exercise. Once lifestyle interventions have been applied, pharmacologic therapy becomes a mainstay of therapy, conventionally with a statin followed by adjunctive medicines as indicated. Certain populations that are refractory to aggressive pharmacotherapy, however - such as patients who have familial hypercholesterolemia (FH) - necessitate alternative means of lipid management. Therapeutic considerations in these patients include LDL apheresis and a number of rare procedures such as partial ileal bypass, liver transplantation, portocaval shunting, and possibly gene therapy in the future. The anti-inflammatory effects of LDL apheresis and its effects on endothelial function are not well known. Considering several pathways of atherogenesis, and inflammation as a central mechanism thereof, LDL apheresis may theoretically provide synergistic benefit of lipid lowering as well as proinflammatory agent lowering that can lead to significantly decreased atherogenesis. This study looks to address these questions by assessing the effects of LDL apheresis on inflammatory and lipid markers.

Official Title
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Inflammatory and Lipid Markers Pre- and Post-LDL Apheresis: A Multicenter Experience

Conditions
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Familial Hypercholesterolemia

Intervention / Treatment
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* Procedure: LDL Apheresis


Participation Criteria
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Eligibility Criteria
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Inclusion criteria: Heterozygous FH with documented CAD and LDL-C ≥ 200 mg/dL Documented CAD may be represented as: Lesion(s) on coronary angiography, history of myocardial infarction, CABG, PTCA, progressive angina demonstrated by stress testing, history of other revascularization procedure (e.g. atherectomy) Homozygous FH and LDL-C > 500 mg/dL Heterozygous FH and LDL-C ≥ 300 mg/dL On stable LDL apheresis therapy for at least 6 months. Exclusion criteria: Patient refusal to participate Inability to attend 2 consecutive LDL apheresis sessions for study duration Subject with advanced renal disease Subject with chronic progressive hepatic disease and demonstrated deficient synthetic function Subject with acute hepatic process Subject with current malignancy Subject with diagnosis of amyloidosis Subject with diagnosis of rheumatoid arthritis Any subject with acute flare of chronic disease Subject with recent ethanol ingestion Subject with significant bone disease

Ages Eligible for Study
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Minimum Age: 3 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Familial hypercholesterolemia<br>Heterozygous FH with documented CAD and LDL-C ≥ 200 mg/dL (Documented CAD may be represented as: Lesion(s) on coronary angiography, history of myocardial infarction, CABG, PTCA, progressive angina demonstrated by stress testing, history of other revascularization procedure) Homozygous FH and LDL-C > 500 mg/dL Heterozygous FH and LDL-C ≥ 300 mg/dL On stable LDL apheresis therapy for at least 6 months | Procedure: LDL Apheresis<br>* LDL Apheresis<br>* Other names: LDL filtration;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Lipid Marker Change | We will measure the level of cholesterol markers in your blood before and after the LDL apheresis procedure with a blood draw. | 1 month | 
| Inflammatory Marker Change | We will measure the level of inflammatory markers in your blood before and after the LDL apheresis procedure with blood draws (for 2 apheresis sessions) | 1 month | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Inflammatory Marker Rebound | We will measure the level of inflammatory markers in your blood after LDL apheresis procedure the following morning, 24 hours after procedure, and on the second morning. | 2 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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LDL apheresis, Familial hypercholesterolemia, Inflammatory markers, Hyperlipidemia, Cardiovascular disease