Isothiocyanates and Metabolic Health

Study Overview
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Brief Summary
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Many sprouts, for example broccoli sprouts, contain substances that have shown to have protective effects, both against oxidative stress and against carcinogenic substances. In this study we will investigate the activation of the body's antioxidant defense system and effect on blood lactate and glucose after 1 week of isothiocyanate rich sprout supplementation. The subjects will perform maximal and submaximal work test, blood sampling, muscle biopsies and blood pressure monitoring post supplementation. We hypothesize that 1 week of supplementation of isothiocyanate rich sprouts alters blood lactate and glucose metabolism and activates the body's antioxidant defense system.

Detailed Description
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Many sprouts, for example broccoli sprouts, contain high levels of glucosinolates, which can be converted to the bioactive substances isothiocyanates. This conversion is assisted by another plant enzyme, myrosinase, which is released when the plant is mechanically processed. It is believed that this reaction is part of the plant's natural defense against pest insects and other animals that attack the plant in the early developmental stage. In cell and animal studies supplementation with isothiocyanates has been shown to induce the protein Nrf2 which is a so-called Master regulator of the body's antioxidant defenses and activates more than 200 genes that together provide a strong protection against oxidative and mutagenic stress. In this study we will investigate Nrf2 activation in human skeletal muscle and effect on lactate-, glucose metabolism after 1 week of isothiocyanate rich sprout supplementation. The study is a double-blinded, randomized, placebo controlled, cross-over study with 4 weeks wash-out between the interventions. We will perform maximal- and submaximal work test and monitor blood glucose and lactate during the tests. Also, in the post intervention phases we will perform muscle biopsies, glucose tolerance tests and monitor blood pressure. The hypothesis is that 1 week of isothiocyanate rich sprout supplementation activates Nrf2 in human skeletal muscle and alters blood lactate and glucose metabolism in rest and during exercise.

Official Title
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Isothiocyanates and Metabolic Health

Conditions
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Metabolic Disturbance

Intervention / Treatment
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* Dietary Supplement: Isothiocyanate rich sprouts
* Dietary Supplement: Placebo Juice


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy volunteer 18-45 years old Exclusion Criteria: Any chronic disease Inability to perform cycling exercise Allergic to anesthesia

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Randomized, placebo controlled, cross-over study
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Isothiocyanate Rich Juice<br>The juices will contain 37,5g isothiocyanate rich sprouts and be delivered frozen to the subjects. The subjects will be instructed to consume two juices per day. | Dietary Supplement: Isothiocyanate rich sprouts<br>* Isothiocyanate rich sprout juice consumption for 1 week<br>|
| Placebo Comparator: Placebo Juice<br>The juices will contain 37,5g alfalfa sprouts and be delivered frozen to the subjects. The subjects will be instructed to consume two juices per day. | Dietary Supplement: Placebo Juice<br>* Sprout juice devoid of isothiocyanates for 1 week<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood lactate | Changes in blood lactate during exercise | Acute during sub maximal exercise | 
| Nrf2 activation | Increased Nrf2 protein in human skeletal muscle | Acute after 1 week of supplementation | 
| Glucose regulation | Improved continuous glucose control | Throughout 1 week of supplementation | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glucose tolerance test | Improved glucose regulation in rest | 120 minutes | 
| Clinic blood pressure | Blood pressure regulation | Acute after 1 week of supplementation | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Isothiocyanates, Blood lactate, Glucose regulation, Nrf2