Analysis of Neutrophil Response in Chronic Periodontitis

Study Overview
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Brief Summary
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Recent scientific evidence suggests that the main defence cell of the body (neutrophil) behaves in a different manner in patients with gum disease; namely how they interact with bacteria and their role in defence systems of the body. This study proposes to examine these responses in periodontitis patients and healthy controls. The proposed study will include patients undergoing periodontal (gum) treatment who will be matched to periodontally healthy controls. Blood, gingival crevicular (gum) fluid and clinical measures will be collected both pre- and post-therapy to measure differences in cell behaviour both before, and following routine therapy. Blood samples will be used to isolate peripheral blood neutrophils prior to analysis of their responses to different bacterial stimuli including oxygen radical, cytokine and extracellular trap release. Gingival Crevicular Fluid samples will be used to measure different biochemical markers that result from the production of Neutrophil Extracellular Traps (NETs). Routine clinical measures will be taken both pre- and post-therapy as a measure of treatment response. Patient volunteers undergoing treatment will be asked to donate a total of four small samples of tissue from the gums whilst they are already anaesthetised for routine treatment. These will be approximately the size of a needle head (2mm2mm) and used to examine NET formation within the tissues and related processes. This will provide novel in-vivo data regarding this recently discovered method of neutrophil defence in innate immunity. This proposal represents a novel study aimed at improving our current understanding of why inflammatory periodontitis develops in some patients but not others, as well as providing pointers to causal/noncausal relationships between periodontitis and important systemic conditions such as diabetes and rheumatoid arthritis. Ultimately, novel treatment approaches and primary prevention (for periodontitis) or secondary prevention (for systemic disease) strategies may emerge.

Official Title
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Analysis of Neutrophil Response in Chronic Periodontitis (Including the Project Entitled 'Pro-Inflammatory Cytokine Production by Neutrophils in Periodontitis Patients')

Conditions
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Chronic Periodontitis

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Be aged 18 or over Have a minimum of 20 teeth Have chronic periodontitis or otherwise healthy volunteers (controls) Be capable of giving informed consent themselves and are able and willing to participate in the study Exclusion Criteria: Patients with aggressive disease Mouthwash use Special dietary needs (e.g. coeliac) Patients with physical or mental disability Pregnant women or those breastfeeding Patients whose medical history may place them at risk of complications from periodontal therapy (e.g. warfarinised volunteers) Patients taking long term anti-microbial or anti-inflammatory drugs Patients unable or unwilling to provide informed consent Taking dietary supplements (e.g. multivitamins, antioxidants, fish oils) Shows unwillingness, inability or lack of motivation to carry out the study procedures, or cannot conform to the protocol A course of antibiotic or anti-inflammatory therapy currently or in the previous 3 months Current orthodontic treatment (e.g. wearing braces or orthodontic devices) Currently participating in another dental trial Diabetics Smokers or have smoked up to within 5 years Those patients who disclose previous or current recreational drug use

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Control volunteers with periodontal health<br>Control volunteers with periodontal health | |
| Patient volunteers with chronic periodontitis<br>Patient volunteers with chronic periodontitis | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Principal Research Question | Do leukocyte (neutrophil) responses differ between patients diagnosed with periodontitis compared to matched healthy controls, and what is the effect of standard periodontal treatment and stabilisation on this response? | Three months for each matched patient and control | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Secondary Research Question | Can markers of the white blood defence cells (neutrophils) be observed and measured in-vivo within the local tissues and if so do they differ between inflamed and non-inflamed sites? | Three months for each matched patient and control | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Periodontitis, Neutrophil, Inflammation