GCF Levels of IL-1β and IL-6 in Gingivitis and Stage III-Grade C Periodontitis

Study Overview
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Brief Summary
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Periodontal diseases are chronic diseases that occur as a result of a violation of the balance between microbial dental plaque and the host response. Gingivitis is a disease characterized by inflammation of the gingiva that occurs in one or more areas without loss of attachments.1 in periodontitis, an inflammatory event that begins in the gingiva along with gingivitis spreads to the periodontal ligament, alveolar bone and soft tissues that support the tooth, causing the destruction of these structures.2 Cytokines are low molecular weight proteins that participate in the initial and active stages of inflammation and immunity. In periodontal disease pathogenesis, cytokine response has been reported to play a very critical role in determining disease progression.3 IL-1beta and IL-6 are key cytokines in chronic inflammatory diseases and have the potential to initiate bone loss and tissue destruction seen in periodontal disease.4the purpose of this study; it is to determine the degree of inflammation and periodontal destruction by determining the levels of IL-1beta and IL-6 cytokines in the gingival crevicular fluid of periodontal healthy and diseased individuals.

Detailed Description
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Periodontal measurement were done at 6 sites per tooth After clinical evaluation, individuals were divided into 3 groups Healthy control groups: clinically healthy gingiva Gingivitis groups: BOP score of 10% or greater and PD≤3mm Periodontitis groups: AL ≥5 mm, PD≥6 mm and tooth loss due to periodontitis of ≤4. GCF samples were collected using strips.

Official Title
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Usak University Faculty of Dentistry

Conditions
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Periodontitis

Intervention / Treatment
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* Diagnostic Test: gingival crevicular fluid


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: have periodontitis have 16 permanent teeth Exclusion Criteria: Systemic disease using drugs

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy control groups<br>clinically healthy gingiva BOP score less than 10% | Diagnostic Test: gingival crevicular fluid<br>* GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.<br>|
| Gingivitis groups<br>BOP score of 10% or greater | Diagnostic Test: gingival crevicular fluid<br>* GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.<br>|
| Periodontitis groups<br>interdental AL ≥5 mm, PD≥6 mm | Diagnostic Test: gingival crevicular fluid<br>* GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurement of IL-1Beta level | GCF was taken interproximal region. | GCF was taken within 1 week after clinical examination | 
| Measurement of IL-6 level in GCF | GCF was taken interproximal region. | GCF was taken within 1 week after clinical examination | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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periodontal disease, periodontitis, gingivitis, cytokines