Sensory Symptoms in Active Vitiligo

Study Overview
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Brief Summary
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This work aims to assess sensory symptoms in early active vitiligo patients (segmental, non-segmental, or mixed) and to measure 3 neuropeptides expression in their lesional skin [neuropeptide Y(NPY), calcitonin gene-related peptide (CGRP), and nerve growth factors (NGF)] to correlate neuropeptide levels, sensory symptoms, and functions, with criteria of disease activity and perceived stress scale.

Detailed Description
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Study Design: Case-control study Population: 45 patients with active vitiligo and 45 patients with stable vitiligo Inclusion criteria: Age: 18 years old and older Sex: both sexes Exclusion criteria: Other hypopigmented diseases such as pityriasis alba, tinea versicolor, lichen sclerosis, post-inflammatory hyperpigmentation, and leprosy as excluded by lack of accentuation by wood's light examination. - Cases who received treatment (systemic or topical) in the last 3 months before sampling. - Neurological or other diseases that cause itching. - Medical history of DM, HTN, or other systemic diseases, history of associated autoimmune diseases. Assessment will be carried as follows: • History of itching or other abnormal neurological sensations as paresthesia and numbness. Aggravating factors (e.g. dryness, hot environment, sun exposure, ..etc), alleviating factors of itching Type of vitiligo (segmental, unilateral, vulgaris). • Assessment of activity by assessing: koebner's phenomenon, confetti depigmentation, trihypochrome areas, poorly defined borders, inflammatory signs (e.g eye inflammation and hearing loss), and leukotrichia. • Assessment of sensory symptoms and signs (fine touch, crude touch, pain, and sweating by starch iodine test) in active vitiliginous patches. • Clinical assessment: the vitiligo area score index (VASI), vitiligo extent scores (VES), vitiligo disease activity score (VIDA) (Feily, 2014) will be assessed in every participant and demographic data

Official Title
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Sensory Symptoms as an Early Manifestation of Active Vitiligo: a Case-control Clinical and Molecular Study

Conditions
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Totest Sensory Symptoms in Active Vitiligo

Intervention / Treatment
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* Diagnostic Test: skin biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Active and stable vitiligo Exclusion Criteria: Diabetic or other associated disease

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active vitiligo<br> | Diagnostic Test: skin biopsy<br>* skin biopsy to be analyzed by ELISA<br>|
| Stable vitiligo<br> | Diagnostic Test: skin biopsy<br>* skin biopsy to be analyzed by ELISA<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Neuropeptide | laboratory test | 3 months |