Effect of Beet-root Juice and PBM Treatments on Muscle Fatigue

Study Overview
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Brief Summary
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The goal of this randomized counterbalanced repeated measures study is to test beet-root juice supplementation and red light therapy augment forearm muscle endurance in apparently healthy older adults over 65 years of age. The main questions seek to answer the following question: Does beet-root juice supplementation improve forearm muscle endurance compared to a placebo supplement? Does red light therapy improve forearm muscle endurance compared to a sham light exposure? Does beet-root juice supplementation in combination with red light therapy improve forearm muscle endurance compared to a placebo-sham light control?

Official Title
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Application of Beet-root Juice Supplementation and Photobiomodulation Treatment on Forearm Muscle Fatigue in Older Adults

Conditions
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Health Care Utilization, Healthy Aging

Intervention / Treatment
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* Dietary Supplement: Beet-root juice
* Radiation: red-light therapy
* Dietary Supplement: Placebo
* Radiation: sham light


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Self-reports performing at least 30 minutes of light to moderate intensity physical activity 3 times per week for the last three months Exclusion Criteria: Currently smoke Have smoked within the last 6 months Are taking taking supplements known to increase blood nitrate levels Have a known kidney disease Have a known liver disease Have a known thyroid condition Have a known gastritis condition Are taking medications that are known to cause complications with nitrate supplementation Have known or reported joint pain within the dominant hand

Ages Eligible for Study
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Minimum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Beet-root juice plus red-light therapy<br> | Dietary Supplement: Beet-root juice<br>* Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a 400mg nitrate once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third 400mg dose of nitrates at least 150 minutes before forearm endurance testing.<br>* Other names: Beet it Sport;Radiation: red-light therapy<br>* Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to 660nm wave length light for 10-minutes.<br>* Other names: MitoMIN;|
| Sham Comparator: Beet-root juice plus sham light therapy<br> | Dietary Supplement: Beet-root juice<br>* Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a 400mg nitrate once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third 400mg dose of nitrates at least 150 minutes before forearm endurance testing.<br>* Other names: Beet it Sport;Radiation: sham light<br>* Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to sham light for 10-minutes.<br>|
| Placebo Comparator: Placebo plus red-light therapy<br> | Radiation: red-light therapy<br>* Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to 660nm wave length light for 10-minutes.<br>* Other names: MitoMIN;Dietary Supplement: Placebo<br>* Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a nitrate depleted placebo once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third dose of the nitrate depleted placebo at least 150 minutes before forearm endurance testing.<br>|
| Active Comparator: Placebo plus sham light therapy<br> | Dietary Supplement: Placebo<br>* Two days prior to the forearm endurance protocol, participants will be provided and instructed to consume a nitrate depleted placebo once per day. On the day of the forearm endurance protocol, the participants will be provided and instructed to consume a third dose of the nitrate depleted placebo at least 150 minutes before forearm endurance testing.<br>Radiation: sham light<br>* Participants will place their dominant forearm 15 centimeters away from the light source and have their dominant forearm exposed to sham light for 10-minutes.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Total forearm work performed | Work is the product of the number of times the forearm is activated multiplied by the amount of force produced by the forearm in kilograms. | During the forearm exercise fatigue protocol lasting up to 45 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Forearm endurance | The duration of time the participant takes to complete the forearm fatigue protocol | During the forearm exercise fatigue protocol lasting up to 45 minutes | 
| Intermuscular coordination | Surface electromyography measurements of muscle activity | During the forearm exercise fatigue protocol lasting up to 45 minutes | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Red light therapy, Forearm muscle endurance, Beet-root juice, Electromyography