Overeating Different Fats and Influence on Muscle Mass and Body Fat Accumulation

Study Overview
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Brief Summary
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To investigate metabolic and molecular response to fatty acid-specific overfeeding in overweight subjects, in relation to changes in ectopic fat, lean tissue mass and insulin sensitivity

Official Title
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Role of Fatty Acids in Skeletal Muscle Hypertrophy and Ectopic Fat Accumulation During Overfeeding (LIPOGAIN-2)

Conditions
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Obesity, Overfeeding, Muscle Mass, Body Composition, Ectopic Fat

Intervention / Treatment
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* Dietary Supplement: PUFA-group
* Dietary Supplement: SFA-group


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Body mass index 25-32 Exclusion Criteria: Type 2 diabetes Type 1 diabetes Kidney disease Liver disease Abnormal clinical chemistry at screening Intense physical exercise > 2 hours per week Use of statins or drugs affecting energy metabolism Use of extreme diets

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Saturated fatty acids (SFA)-group<br>Weight gain created by addition of muffins baked on saturated fat | Dietary Supplement: SFA-group<br> <br> |
| Experimental: Polyunsaturated fatty acids (PUFA)-group<br>Weight gain created by addition of muffins baked on polyunsaturated fat | Dietary Supplement: PUFA-group<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in lean tissue mass | Assessed by magnetic resonance imaging (MRI) and air displacement plethysmography (BodPod) | 10 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in hepatic fat content | Assessed by magnetic resonance imaging (MRI) | 10 weeks | 
| Change in visceral adipose tissue (VAT) | Assessed by magnetic resonance imaging (MRI) | 10 weeks | 
| Change in total adipose tissue | Assessed by magnetic resonance imaging (MRI) | 10 weeks | 
| Change in pancreatic fat | Assessed by MRI | 10 weeks | 
| Change in insulin sensitivity | Assessed by oral glucose tolerance test (OGTT) | 10 weeks | 
| Change in plasma lipids | Plasma cholesterol, low-density lipoprotein, high-density lipoprotein, triglycerides, apolipoprotein B, apolipoprotein A1, free fatty acids, glycerol, proprotein convertase subtilisin/kexin type 9 | 10 weeks | 
| Change in circulating inflammation and coagulation markers | Plasma tumor necrosis factor-receptor 2, Interleukin receptor 1 antagonist, C-reactive protein, fibrinogen, adhesion molecules | 10 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Overfeeding, Weight gain, Liver fat, Pancreatic fat, Ectopic fat, Skeletal muscle, Adipose tissue, Gene expression, DNA methylation, Palmitate, Linoleic acid, Saturated fat, Polyunsaturated fat, Metabolism, Insulin sensitivity