Mitochondrial Remodeling After Exercise

Study Overview
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Brief Summary
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Regulation of mitochondrial health in overweight and obese individuals may be impaired. The purpose of this study is to identify impairments in regulation of mitochondrial health within skeletal muscle and to determine if short-term exercise training (2-weeks) can reverse such impairments. The investigator's hypothesis is that pathways that serve to degrade poorly functioning mitochondria in overweight and obese individuals are down-regulated, but that short-term exercise training can restore these pathways to improve skeletal muscle mitochondrial function.

Official Title
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Mitochondrial Remodeling After Exercise

Conditions
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Overweight and Obesity, Insulin Resistance, Metabolic Disease, Mitochondrial Metabolism, Sedentary Lifestyle

Intervention / Treatment
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* Behavioral: Short-term Exercise Training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 18-45 years Body mass index (BMI) 18-45 kg/m2 Sedentary (<1 hour of planned exercise per week) Exclusion Criteria: Regular exercise (>1 hour of planned exercise per week) Smoking, tobacco or nicotine use within the last 1-year Fasting glucose >126mg/dL Hypertension (systolic pressure >140 mmHg or diastolic pressure >90 mmHg) Chronic metabolic or cardiovascular health conditions Pregnant, nursing, irregular menses or post-menopausal Lidocaine allergy Certain medications Physical limitations that prevent safe or successful exercise completion Diminished capacity for consent

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Participants will be asked to complete 2 screening visits to determine eligibility. If participants remain eligible, they will be asked to complete 2 metabolic study visits, 1 before and 1 after 2 weeks of supervised exercise training on a stationary bike.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: Pre-training Metabolic Study Visit<br>Participants will first complete a pre-training metabolic study visit. | |
| Experimental: Post-training Metabolic Study Visit<br>Participants will complete a second, identical metabolic study visit following 2-weeks of exercise training. | Behavioral: Short-term Exercise Training<br>* Participants will perform 2-weeks of supervised exercise training on a stationary bike.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in skeletal muscle mitochondrial protein degradation peptide products | Skeletal muscle mitochondrial protein degradation peptide products after 2-weeks of exercise training compared with before training. | After 2-weeks of exercise training compared with before training. |