Bioavailability of Chemopreventive and Nutritional Compounds in Broccoli

Study Overview
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Brief Summary
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The aim of this study was to determine the absorption of isothiocyanates (ITC) after ingestion of kailan-hybrid broccoli through the analysis of the correspondent urinary biomarkers. Furthermore, the effect of cooking (microwave) on the mentioned metabolic fate of these ITC was studied comparing to the uncooked vegetable.

Detailed Description
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Background Glucosinolates (sulphur-containing compounds) are hydrolyzed by the plant enzyme myrosinase to ITC, which have chemopreventive properties. No information has been reported about the metabolic fate of ITC in the kailan-hybrid broccoli, which physicochemical characteristics could greatly influence that metabolic fate. Previous studies have shown that cooking process of vegetables may vary the human absorption of the correspondent nutritional and bioactive compounds. Research Methods & Procedures A cross-over-design study of 7 subjects after ingestion of 200-g raw or microwaved kailan-hybrid broccoli was conducted. Total ITC equivalents in the vegetable, urine and blood samples were determined by HPLC as the cyclocondensation product of 1,2-benzenedithiol.

Official Title
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Bioavailability of Bioactive Compounds in Broccoli

Conditions
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Bioavailability

Intervention / Treatment
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* Other: raw broccoli


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subjects with a body mass index between 19 and 28. Subjects age: 22-50 years-old. Good health of subjects: confirmed by medical history, physical examination and clinical laboratory analysis of urine and blood (complete blood count and liver function markers including serum glutamic-pyruvic transaminase bilirubin test and glucose-6-phosphate dehydrogenase). Exclusion Criteria: Tobacco smoking. Use of regular medications. Had taken antibiotics for 4 weeks immediately preceding the study. History of kidney stones, diabetes mellitus, bleeding disorders or family history of iron overload/hemochromatosis.

Ages Eligible for Study
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Minimum Age: 22 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Screening
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Raw<br>Broccoli,200 grams | Other: raw broccoli<br>* To eat this plant material<br>* Other names: Kailan hybrid;|
| Experimental: cooked<br>Microwaved, 200 grams | Other: raw broccoli<br>* To eat this plant material<br>* Other names: Kailan hybrid;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolic fate of ITC of the kailan-hybrid broccoli after human comsuption | Glucosinolates (sulphur-containing compounds) are hydrolyzed by the plant enzyme myrosinase to isothiocyanates (ITC), which have chemopreventive properties. Previous studies have shown that cooking process of vegetables may vary the human absorption of the correspondent nutritional and bioactive compounds. | Up to 4 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Broccoli; Isothiocyanates; Pharmacokinetics; Sulforaphane; Myrosinase