Investigation of Asprosin a Novel Adipokine in Periodontitis

Study Overview
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Brief Summary
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Asprosin, a recently discovered glucogenic adipokine, is mainly synthesized by white adipose tissue and released during fasting. Appetite, glucose metabolism, insulin resistance, cell apoptosis, etc. asprosin is associated with diseases such as diabetes, obesity, polycystic ovary syndrome, and cardiovascular diseases. Periodontal tissue may act as a source of endocrine-like inflammatory mediators (such as TNF-α, IL-6 and IL-1) that are important in periodontal inflammation and can affect glucose and lipid metabolism. Production of TNF-α and IL-6 in adipose tissues strengthens the relationship between obesity, T2DM and periodontitis.we postulated that asprosin may be candidate for explaining the triangular relationship among obesity, T2DM, and periodontal disease.

Detailed Description
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Periodontal disease is a chronic, multifactorial, and infectious disease caused by bacteria. It is characterized by the formation of an inflammatory response in the supporting bone and connective tissue against microbial dental plaque, and the nature of the resulting inflammatory response determines the course of periodontal disease. Type 2 Diabetes Mellitus (T2DM), obesity, and periodontal disease are closely related, presenting a triad association. Obesity is the crucial cause of T2DM and periodontitis is the sixth complication of diabetes. Asprosin circulates in the blood at nanomolar levels and is taken to the liver, where it activates the G protein-cAMP-PKA pathway, causing rapid glucose release into the circulation. Insulin-resistant humans and mice have been reported to have pathologically elevated plasma asprosin levels. It will be investigated whether asprosin, which we know to be effective on glucose metabolism, is affected by the periodontal condition.

Official Title
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Serum and Saliva Asprosin Levels Are Associated With Periodontitis

Conditions
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Periodontitis, Obesity, Diabetes Mellitus

Intervention / Treatment
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* Diagnostic Test: Serum and salivary samples will be collected. Asprosin levels will be determined by biochemical analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - All individuals were generally healthy, non-smoking Exclusion Criteria: Pregnant or breastfeeding women None had undergone periodontal therapy and/or antibiotic therapy in the past 6 months. None has a contagious disease such as HIV or AIDS

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Periodontitis group<br>35 subjects had periodontitis. Periodontitis will be staged by including patients with a periodontal pocket (PD) measurement of 4 mm and above, accompanied by attachment loss and radiographic bone loss. | Diagnostic Test: Serum and salivary samples will be collected. Asprosin levels will be determined by biochemical analysis<br>* Serum and saliva samples will be collected from both groups for biochemical analysis.<br>|
| Healthy group<br>30 subjects had healthy periodontal tissue.Healthy controls included volunteers with clinically healthy gingiva on an intact periodontium who had BOP < 10% and PD ≤ 3 mm, no sites with attachment loss, no radiographic sign of alveolar bone destruction, and no history of periodontitis. | Diagnostic Test: Serum and salivary samples will be collected. Asprosin levels will be determined by biochemical analysis<br>* Serum and saliva samples will be collected from both groups for biochemical analysis.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Asprosin Levels | Determination of asprosin levels by making biochemical analyzes from serum and saliva samples obtained | two weeks |