Tissue Lesions in Exercise Related Ischemia

Study Overview
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Brief Summary
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The purpose of this study is to evaluate the effect of muscle ischemia during claudication on mitochondrial function. Comparison to patients with arterial disease but absence of lower limb claudication (Cardiac group) feasibility study.

Detailed Description
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Assess the feasibility of the study of micro-vascular function and mitochondrial on muscle sample (sartorius) in a group of arterial disease and non arteriopathy group. Analysis of the activity of mitochondrial chain Immunochemistry of tissue markers

Official Title
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Atteinte Tissulaire au Cours de l'ischémie Chronique d'Effort. Validation d'un modèle d'étude du Muscle Sartorius Humain

Conditions
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Ischemia, Peripheral Artery Disease

Intervention / Treatment
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* Other: Muscle Biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Intervention for femoropopliteal bypass (PAD group) Intervention for saphenous withdrawal for coronary bypass surgery (cardiac group) Exclusion Criteria: Participate in another biomedical research Pregnant woman

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Peripheral artery diseased group<br>pad Patients referred for femoro popliteal bypass Muscle biopsy during surgery | Other: Muscle Biopsy<br>* A distal and proximal biopsy of the sartorius muscles ( 0.5 cm3 each) will be performed during surgery for saphenous vein withdrawal (cardiac group) or arterial bypass (PAD group) The samples are then treated with neurovascular and mitochondrial biology laboratory. the activity of the complex 1 is analyzed and compared between the 2 groups.<br>|
| Sham Comparator: Cardiac group<br>Patients referred for saphenous withdrawal for coronary bypass Muscle biopsy during surgery | Other: Muscle Biopsy<br>* A distal and proximal biopsy of the sartorius muscles ( 0.5 cm3 each) will be performed during surgery for saphenous vein withdrawal (cardiac group) or arterial bypass (PAD group) The samples are then treated with neurovascular and mitochondrial biology laboratory. the activity of the complex 1 is analyzed and compared between the 2 groups.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Activity differences in mitochondrial complex I between the distal and proximal levy in group 1 and group 2. | Measurement of mitochondrial complex 1 activity (arbitrary units) on muscle biopsies by the laboratory of biochemistry. Comparison of the results observed on the same miuscle at the proximal level (assumed normal in both groups) and distal level (assumed ischemic in claudicants but not in cardiac patients without limb symptoms. | 1 week |