Neurohumoral Mechanism of Deferent Frequency Transcutaneous Electrical Acupoint Stimulation(TEAS)

Study Overview
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Brief Summary
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The purpose of this study is to explore the neurohumoral mechanism of deferent frequency TEAS pretreatment.

Detailed Description
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Patients were randomly assigned to three groups, receiving TEAS 30min before anesthesia. The frequency of high frequency group is set to 10/50HZ, low frequency group is 2/10HZ, and the fake stimulation group only attach electrodes without electric current. Acupoint selection is bilateral Neiguan points(PC6). Venous blood is collected 4ml before and immediately after TEAS respectively. Heart rate variability is monitored continuously during the 30min TEAS. The adverse events recorded.

Official Title
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Neurohumor Mechanism of Deferent Frequency Transcutaneous Electrical Acupoint Stimulation(TEAS): A Prospective, Randomized Controlled Trial

Conditions
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Hypotension

Intervention / Treatment
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* Device: TEAS (transcutaneous electrical acupoint stimulation)
* Device: TEAS (transcutaneous electrical acupoint stimulation)
* Device: fake stimulation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 18yrs-65yrs ASA 1-2 Scheduled for elective operation under general anesthesia BMI 18-30kg/m2 Informed consented Exclusion Criteria: Contraindications to TEAS Communication difficulty with researchers Addicted to alcohol, analgesics or other drugs Cardiac insufficiency or high blood pressure without controlled Hepatic or renal dysfunction Participate in the other clinical trial 3 month before the enrollment

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: 2/10HZ TEAS pretreatment<br>Patients were given 30min of 2/10HZ TEAS before anesthesia | Device: TEAS (transcutaneous electrical acupoint stimulation)<br>* 2/10HZ Electric stimulation was given through electrode attached to specific acupoints<br>|
| Experimental: 10/50HZ TEAS pretreatment<br>Patients were given 30min of 10/50HZ TEAS before anesthesia | Device: TEAS (transcutaneous electrical acupoint stimulation)<br>* 10/50HZ Electric stimulation was given through electrode attached to specific acupoints<br>|
| Sham Comparator: fake stimulation<br>Patients were only attached electrodes without electric current | Device: fake stimulation<br>* Patients were only attached electrodes without electric current<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma concentration of epinephrine(ng/L) |  | change of TEAS 0 and 30 minutes | 
| Plasma concentration of norepinephrine(ng/L) |  | change of TEAS 0 and 30 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Heart rate variability |  | During 30min TEAS | 
| plasma concentration of glucocorticoid(μg/L) |  | change of TEAS 0 and 30 minutes | 
| plasma concentration of aldosterone(ng/L) |  | change of TEAS 0 and 30 minutes | 
| plasma concentration of renin(ng/L) |  | change of TEAS 0 and 30 minutes | 
| plasma concentration of β-endorphin(ng/L) |  | change of TEAS 0 and 30 minutes |