Nicotine Reinforcement and Smoking-Cue Reactivity: Association With Genetic Polymorphisms

Study Overview
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Brief Summary
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In this study, potential associations between several genetic polymorphisms and nicotine dependence will be examined. The relative reinforcing efficacy of cigarettes using the forced-choice procedure will be assessed. In addition, reactivity to smoking cues using a reliable procedure will be conducted.

Detailed Description
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It is known that cannabinoid and dopamine receptors can be important modulators of response to nicotine and its associated cues. In this study participants will be genotyped to determine whether there is a relationship between CB1 or D3 genes and the reinforcing properties of nicotine or the subjective effects of nicotine-associated cues. In one session, participants will chose between two different cigarettes, each with different properties. In another session, physiological responses and craving will be measured when presented with cues that remind participants of nicotine. At some point during the study, blood will be collected for genotyping and this will processed to study whether CB1 or D3 genotype influences these responses to nicotine or cues.

Official Title
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Nicotine Reinforcement and Smoking-Cue Reactivity: Association With Genetic Polymorphisms

Conditions
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Cigarette Smoking Behavior

Intervention / Treatment
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* Genetic: Genotype dopamine
* Genetic: Genotype cannabinoids


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 19-64 year old males and females smoking at least 10 cigarettes per day for at least 1 year positive urinary cotinine medically and psychologically healthy as determined by screening criteria Exclusion Criteria: definite plan to reduce or quit tobacco use during the course of the study treatment for tobacco dependence in the past 3 months use of nicotine replacement products, bupropion, or varenicline in the past 3 months consumption of more than 15 alcoholic drinks per week during the past month use of any illicit drug more than twice per week during the past month current use of any medication that would interfere with the protocol in the opinion of PI under the influence of a drug or alcohol at any session pregnant, nursing, or become pregnant during the study

Ages Eligible for Study
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Minimum Age: 19 Years
Maximum Age: 64 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Genotype dopamine<br>Genotyping will be conducted at the conclusion of the study | Genetic: Genotype dopamine<br>* Laboratory investigations of nicotine reinforcement<br>Genetic: Genotype cannabinoids<br>* Laboratory investigations of nicotine reinforcement<br>|
| Genotype cannabinoids<br>Genotyping will be conducted at the conclusion of the study | Genetic: Genotype dopamine<br>* Laboratory investigations of nicotine reinforcement<br>Genetic: Genotype cannabinoids<br>* Laboratory investigations of nicotine reinforcement<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of puffs on a cigarette | Puffs taken from either a nicotinized or a denicotinized cigarette | 3 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Craving in response to nicotine cues | Craving scores measured by questionnaires in response to either smoking-related or neutral cues | 3 years | 
| Physiological measures in response to cues | Physiological measures (eg., heart rate and/or blood pressure) in response to smoking-related or neutral cues | 3 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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genes, cues, reward, smoking