Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein (ICADP)

Study Overview
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Brief Summary
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10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin. The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin. Hypotheses: I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period. II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.

Official Title
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Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein

Conditions
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Muscle Protein Synthesis, Amino Acid

Intervention / Treatment
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* Dietary Supplement: Meat Hydrolysate
* Dietary Supplement: Cluster Dextrin
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: young healthy adults, organised sport/training 1-3 times per week Exclusion Criteria: Subject younger than 18 years or above 30 years BMI > 30 Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus. Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week. Participating in systematized exercise more than 3 times per week. Vegetarian. Participating in a special diet which deviates from the standard of young adults.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Meat hydrolysate & Cluster Dextrin<br>Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin. | Dietary Supplement: Meat Hydrolysate<br>* 25 grams of meat hydrolysate to maximally stimulate protein synthesis.<br>Dietary Supplement: Cluster Dextrin<br>* 75 grams of Cluster Dextrin<br>* Other names: Glico Nutrition Co., Ltd.;|
| Active Comparator: Meat hydrolysate & placebo<br>Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose. | Dietary Supplement: Meat Hydrolysate<br>* 25 grams of meat hydrolysate to maximally stimulate protein synthesis.<br>Dietary Supplement: Placebo<br>* 75 grams of Glucose<br>* Other names: Glucose;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Protein hydrolysate amino acid label time to peak appearance in plasma | Difference between the time to peak of protein-derived D5-phenylalanine amino acid appearance in the plasma | From hour 0 to hour 3 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise | Difference between myofibrillar protein fractional synthesis rate after meat hydrolysate with either 75 grams of Cluster Dextrin or Clucose. | from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3. | 
| Protein hydrolysate amino acid label area under the concentration curve in plasma | Difference between the area under the concentration curve of protein-derived D5-phenylalanine amino acid appearance in the plasma | From hour 0 to hour 3 | 
| Insulin concentration | Difference between insulin concentrations after intake of the two carbohydrate types. | From hour 0 to hour 3 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Carbohydrate, Cluster Dextrin, Exercise training, Protein supplement