Alcohol Induced de Novo Lipogenesis in Women

Study Overview
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Brief Summary
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The study is cross-sectional is design. In the first Aim of this study, 5 women and 5 men will be asked to consume 2g/kg of [2H]water (a.k.a. deuterium oxide or heavy water) which incorporates 2H tracers into newly synthesized fatty acids and triglycerides. Deuterated water is not radioactive and has a long history of application in human studies. Researchers will collect blood samples 2 hours before and 5 hours after the participants consume 20 grams of alcohol as vodka to measure alcohol induced hepatic de novo lipogenesis (DNL) in both men in women. In addition, for Aim 2 researchers will recruit an additional 10 women who will be randomized into one of two groups who will consume a beverage containing vodka and sucrose, or sucrose alone. Aim 2 will be identical to the experimental scheme in Aim 2 in order to determine if sucrose enhances the effects of vodka on hepatic DNL.

Official Title
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Alcohol Induced de Novo Lipogenesis in Women

Conditions
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Liver Metabolism

Intervention / Treatment
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* Other: ETOH
* Other: Sucrose


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male or Female Ages 21 - 45 Healthy Light to moderate alcohol consumption (≤1 drink a day ( 14g) in women and ≤2 drinks a day ( 28 g) in men) BMI between 18.5-29.9 Normal Nutritional Status Ability to speak and understand English Exclusion Criteria: BMI >29.9 Comorbid conditions Any regular medications, except Oral Contraceptives Pregnant or Breastfeeding Regular exercise above activities of daily living

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: A. ETOH<br>5 Males and 5 Females will receive deuterated water and drink 2.15 ounces of alcohol in the form of vodka and have de novo Lipogenesis measured at 11 time points during and after. | Other: ETOH<br>* Participants will consume Vodka<br>* Other names: Vodka;|
| Experimental: ETOH + Sucrose<br>5 Females will receive deuterated water and drink 1.72 ounces of alcohol in the form of vodka + 7 grams of sucrose and have de novo Lipogenesis measured at 11 time points during and after. | Other: ETOH<br>* Participants will consume Vodka<br>* Other names: Vodka;Other: Sucrose<br>* Participants will consume sucrose in solution with water<br>* Other names: Sucrose solution;|
| Experimental: C. Sucrose<br>5 Females will receive deuterated water and drink a solution containing water and 35 grams of sucrose, and have de novo Lipogenesis measured at 11 time points during and after. | Other: Sucrose<br>* Participants will consume sucrose in solution with water<br>* Other names: Sucrose solution;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Mean Change in Hepatic De Novo Lipogenesis (DNL) | DNL will be measured by Orbitrap high-resolution-mass-spectrometry (GC-Orbitrap-HRMS) We will measure changes in the incorporation of 2H tracers into plasma triglycerides over time. This data will be analyzed by averaging each time point and looking at change in de novo lipogenesis over time. | 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6, and 7 hours post heavy water consumption |