Transcriptome Profiling and Endometrial Receptivity

Study Overview
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Brief Summary
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The aim of this study is to correlate and identify the biochemical markers which may reflect WOI. Blood samples will be collected and endometrial biopsy will be performed at particular time points.

Detailed Description
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Endometrial receptivity in the midluteal phase of the menstrual cycle, known as the window of implantation (WOI), occurs only for a very short period of time. However, so far, there is no reliable clinical and biochemical indicators of WOI. Therefore, this study is to correlate and identify the biochemical markers which may reflect WOI. participants will be asked to be: performed endometrial biopsy taken blood for a few time points (e.g. pre-conception and being pregnant) and to compare different kinds of biochemical markers.

Official Title
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Transcriptome Profiling and Immune/ Biochemical Markers of Peripheral Blood to Predict Endometrial Receptivity and Successful Implantation

Conditions
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Pregnancy Related

Intervention / Treatment
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* Procedure: endometrial biopsy and blood sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 20-40 years. Exclusion Criteria: Uncorrected uterine anomalies, such as septate or bicornuate uterus, fibroids (submucous/intramural). Intrauterine adhesions. Adenomyosis.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Procedure: endometrial biopsy and blood sampling|patient will be performed endometrial biopsy and collected blood samples at particular time points|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The change of the transcriptome profiling in the peripheral blood | RNA per sample will be prepared into two portions. One is for RNA sequencing and the other is for small RNA sequencing. The next step is hydrolysis of RNA into 200-300 nucleotides prior to reverse transcription. When sequencing RNA other than mRNA the library preparation is modified. The cellular RNA is selected based on the desired size range. For miRNA, the RNA is isolated through size selection. Once isolated, linkers are added to the 3' and 5' end then purified. The final step is cDNA generation through reverse transcription. | From the day of endometrial biopsy through study completion, an average of 2 months | 
| The change of the immune markers in the peripheral blood | Immune markers including cytokines such as leukemia inhibitory factor, macrophage colony stimulating factor, interleukin - 1 beta, transforming growth factor beta-1, Tim-3, galectin-9 and other markers considered to play a role in implantation will also be measured. | From the day of endometrial biopsy through study completion, an average of 2 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Transcriptome, endometrial receptivity