PTEN-AKT-FOXO3 Gene Expression Relation With Low-ovarian Reserve in Endometriomas

Study Overview
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Brief Summary
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In this study, we aimed to investigate the relationship between PTEN-AKT-FOXO3 gene expression and decrease in ovarian reserve in the presence of endometrioma and other benign ovarian pathologies.

Detailed Description
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It is known that the presence of endometrioma negatively affects ovarian reserve. The development of endometriosis and the effect of the presence of endometrioma on ovarian reserve due to decreased PTEN expression are considered. This study was designed to compare ovarian PTEN-AKT-FOXO3 gene expression and ovarian reserve in patients with underwent surgery caused by endometrioma and those who had ovarian pathology due to other benign causes and PTEN-AKT-FOXO3 gene expression level will be evaluated by RT-PCR analysis.

Official Title
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RELATIONSHIP OF LOW-OVER RESERVE WITH PTEN-AKT-FOXO3 GENE EXPRESSION IN THE PRESENCE OF ENDOMETRIOMAS

Conditions
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Ovarian Reserve, Endometrioma, PTEN-AKT-FOXO3 Gene Expression

Intervention / Treatment
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* Genetic: PTEN-AKT-FOXO3 Gene Expression


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 1) To be in the range of 18-40 years 2) Patients with an operation indication with >=5 cm endometrioma 3) Patients with operation plan due to non-endometrioma ovarian pathologies Exclusion Criteria: 1)Patients over the age of 40 2)Patients with suspected malignancy 3)Follow-up patients without indications for operation

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| PATİENCE GROUP<br>To be in the range of 18-40 years Patients with an operation indication with >=5 cm endometrioma Symptomatic patients due to endometrioma (dysmenorrhea, dyspareunia, chronic pelvic pain, etc.) Patients who will be followed for IVF cycle due to infertility | Genetic: PTEN-AKT-FOXO3 Gene Expression<br>* In the patient and patient control groups, 0.5 mm tissue strip will be removed for pathology from the cyst wall removed during surgery. and tissue samples will be stored in phosphate buffered saline at -80 C and PTEN-AKT-FOXO3 gene expression level will be evaluated by RT-PCR analysis.<br>|
| PATİENCE-CONTROL GROUP<br>To be in the range of 18-40 years Patients with operation plan due to non-endometrioma ovarian pathologies Symptomatic patients with benign ovarian pathology (such as chronic pelvic pain, compression, syphilomas, dysmenorrhea, dyspareunia, etc.) Asymptomatic, despite a 6-month follow-up period, increase in cyst size or become symptomatic | Genetic: PTEN-AKT-FOXO3 Gene Expression<br>* In the patient and patient control groups, 0.5 mm tissue strip will be removed for pathology from the cyst wall removed during surgery. and tissue samples will be stored in phosphate buffered saline at -80 C and PTEN-AKT-FOXO3 gene expression level will be evaluated by RT-PCR analysis.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| PTEN AKT FOXO3 gene expression | In the patient and patient control groups, 0.5 mm tissue strip will be removed for pathology from the cyst wall removed during surgery. and tissue samples will be stored in phosphate buffered saline at -80 C and PTEN-AKT-FOXO3 gene expression level will be evaluated by RT-PCR analysis. | 30 minutes |