Adipose Tissue and Polycystic Ovary Syndrome (PCOS)(EIFFEL)

Study Overview
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Brief Summary
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The purpose of this study is to collect data to help understand why some women develop Polycystic Ovary Syndrome (PCOS) associated with decreased lower-body fat.

Detailed Description
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Epigenetics may represent a new regulator mechanism explaining gluteal vs. abdominal fat differences.

Official Title
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Adipose Tissue Angiogenesis in Polycystic Ovary Syndrome (PCOS)

Conditions
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Polycystic Ovary Syndrome, Metabolic Diseases, Overweight

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Female Age > 20 and < 40 Weight stable (change of less than 3 kg in the last 8 weeks) BMI < 40 kg/m2 or > 27 kg/m2 Supplemental inclusion criteria for PCOS women: NIH criteria - confirmed by subjects' medical records. Exclusion Criteria: Male Women who are pregnant or lactating (breast feeding) Post-menopausal women Women with hysterectomy Diagnosed with diabetes, or have a fasting blood sugar > 126 mg/dL. Untreated or symptomatic thyroid disease. Impaired kidney or liver function, as evidenced by your blood work Hypertension/ high blood pressure or are taking blood pressure medications Use of oral contraceptives or hormone replacement therapy. History of drug or alcohol abuse (> 3 drinks per day) in the last 5 years, or psychiatric disease prohibiting adherence to study protocol. History of cancer within the last 5 years. History of organ transplant. History of HIV, active Hepatitis B or C, or Tuberculosis. History of heart attack/ myocardial infarction. Presence of clinically significant abnormalities on EKG. Current smokers (smoking within the past 3 months) Use of any medications known to influence glucose, fat and/or energy metabolism within the last 3 months (e.g., growth hormone therapy, glucocorticoids [steroids], etc.). Metformin for women with PCOS is allowed.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| PCOS group<br>12 women with Polycystic Ovary Syndrome (PCOS) as defined by NIH criteria | |
| Pear shapes<br>12 with an android pattern as defined by a waist-to-hip greater than 0.85 | |
| Apple shapes<br>12 will have a gynoid pattern as defined by a waist-to-hip ratio less than 0.78 | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Difference of angiogenesis capacity in abdominal and gluteal adipose tissue | The angiogenesis capacity of each adipose tissue depot will be determine by an in vitro angiogenesis test. | Day 7 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Presence of biomarkers in abdominal and gluteal adipose tissue in obese and PCOS women | Measurement will be obtained with Fluorescence-activated cell sorting (FACS). Identify different and similar biomarkers in obese and PCOS women, from abdominal and gluteal adipose tissue samples. Total RNA, micro RNA and DNA will be extracted from each type of cells using FACS-cell sorting analysis. | Day 7 | 
| Difference in oxygen content of abdominal and gluteal adipose tissue | Adipose tissue oxygen content will be measured using the Licox CMP microprocessor. | Day 6 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Women, Abdominal Adipose, Gluteal Adipose, Polycystic Ovary Syndrome, Obesity