Phase 1 Study of the Effects of Combining Topical FDA-approved Drugs on Age-related Pathways on the Skin of Healthy Volunteers

Study Overview
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Brief Summary
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This is a phase 1 study in healthy adult volunteers to examine the effects of 3 FDA approved medications on skin aging when applied in topical form. This is an open label, placebo controlled study.

Detailed Description
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The primary endpoint of the study is the profile of differences in transcript levels of age-associated genes such as those in the lamin-A, insulin like growth factor (IGF) and NFKB pathways as well as noncoding RNAs in topical agent-exposed arm skin versus placebo exposed arm skin in healthy volunteers. The secondary endpoints include (1) differences in skin wrinkling using a 4 point Likert scale for wrinkle severity between placebo and topical agent exposed arm skin after 4 weeks of usage; (2) the type and severity of adverse events, both systemic and skin localized after exposure to both topical agent and placebo vehicle cream.

Official Title
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Phase 1 Study of the Effects of Combining Topical FDA-approved Drugs on Age-related Pathways on the Skin of Healthy Volunteers

Conditions
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Aging

Intervention / Treatment
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* Drug: Sirolimus
* Drug: Metformin
* Drug: Diclofenac


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age greater than or equal to 55 years Female All 4 grandparents of European descent Fitzpatrick Skin type 1-3 (fair skin type) Presence of moderate to severe fine wrinkling on arm skin Presence of moderate to severe dyspigmentation on arm skin Normal or overweight body mass index Exclusion Criteria: History of laser treatment or chemical peels to to arm History of topical anti-aging products (includes retinol, tretinoin, tazarotene, azelaic acid, hydroquinone) to skin within 6 weeks of starting study History of surgical procedures to arm skin area including removal of benign or malignant skin cancers in the area of topical study agent application Current skin conditions in the area of arms including seborrheic keratosis, rosacea, eczema that may obscure study assessment History of abnormal scarring Uncontrolled medical problems including concurrent infection or malignancy at time of enrollment Unable to provide and sign written informed consent Unable to comply with study-related procedures including keeping study diary, application of topical study agents, avoidance of direct sun exposure >5 minutes per day or ultraviolet tanning bed usage Not willing to provide two small skin biopsies at end of study Known allergy to sirolimus, diclofenac or metformin. Known immunosuppression including organ transplant, HIV, autoimmune disease, and chronic leukemia or lymphoma Fasting blood sugar above the upper limit of normal for Stanford laboratory Diarrhea Use of greater than one alcoholic beverage per day Stress such as surgery or trauma within 2 weeks of enrollment Liver disease such as hepatitis B or C Planning to embark on dieting, caloric restriction or new exercise regimen during the study to lose weight. Unable to refrain from using any topical agent on arms besides the study agents provided for duration of study. Hematocrit, hemoglobin, platelets, white blood cell count, serum Na, K, Cr, aspartate aminotransferase (AST), alanine aminotransferase (ALT) greater or lesser than 1.5 times limits of normal. History of heart failure, coronary artery disease, including angina, coronary artery bypass graft, pacemaker or stent placement History of bleeding or ulcers of the gastrointestinal tract History of diabetes mellitus Current use of blood thinners (includes heparin, warfarin and aspirin) Current use of strong cytochrome P 450 (CYP) 3A4 inhibitor and/or P-gp (examples include ketoconazole, voriconazole, itraconazole, erythromycin, clarithromycin) or strong inducers of CYP3A4 and/or P-gp (examples include rifampin or rifabutin) Abnormal lipid panel as defined by upper limit of normal at Stanford laboratory

Ages Eligible for Study
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Minimum Age: 55 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Sequential Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Sirolimus, metformin, diclofenac<br>First five enrolled participants | Drug: Sirolimus<br>* Topical sirolimus applied to the skin<br>Drug: Metformin<br>* Topical metformin applied to the skin<br>Drug: Diclofenac<br>* Topical diclofenac applied to the skin<br>|
| Other: Metformin, diclofenac<br>Sixth to tenth enrolled participants | Drug: Metformin<br>* Topical metformin applied to the skin<br>Drug: Diclofenac<br>* Topical diclofenac applied to the skin<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Profile of gene transcript changes | Profile of gene transcript changes | 4 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Wrinkle score | on Likert scale | 4 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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aging