Biomarkers in Stored Tumor Samples From Younger Patients With Liver Cancer

Study Overview
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Brief Summary
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RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at biomarkers in stored tumor samples from younger patients with liver cancer.

Detailed Description
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OBJECTIVES: To characterize, at a molecular level, archived samples of tissue from young patients with fibrolamellar carcinoma and hepatocellular carcinoma in non-cirrhotic livers matched for age and sex. To perform genomic analysis on these tissue samples using array comparative genomic hybridization. To perform targeted gene mutation analysis on these samples by PCR. To perform proteomic profiling on fixed tissues in these samples by various proteomic methods, including IHC and mass spectrometry. To look for association between molecular aberrations and clinicopathologic features in these samples. OUTLINE: Archived tissue samples are collected from the pathology department at Vanderbilt University Medical Center and from the Mayo Clinic in Rochester, Minnesota. Tissue samples are analyzed by genomic analysis using array comparative genomic hybridization, target gene mutation analysis by PCR, and proteomic profiling on fixed tissues using various proteomic methods, including IHC and mass spectrometry. Samples are also examined for association between molecular aberrations and clinicopathologic features found in each disease. Clinical patient data (i.e., age, sex, race, date of diagnosis, risk factors, histology, surgical staging, follow-ups, date of death, and adjuvant therapy) are also collected.

Official Title
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Molecular Analysis of Liver Cancer

Conditions
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Liver Cancer

Intervention / Treatment
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* Genetic: comparative genomic hybridization
* Genetic: molecular genetic technique
* Genetic: mutation analysis
* Genetic: polymerase chain reaction
* Genetic: proteomic profiling
* Other: immunohistochemistry staining method
* Other: laboratory biomarker analysis
* Other: mass spectrometry
* Other: medical chart review


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: DISEASE CHARACTERISTICS: Diagnosis of fibrolamellar carcinoma or hepatocellular carcinoma in a non-cirrhotic liver Archived tumor specimens available for analysis from Vanderbilt University or Mayo Clinic Exclusion Criteria: Not specified PATIENT CHARACTERISTICS: Not specified PRIOR CONCURRENT THERAPY: Not specified
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Genetic: comparative genomic hybridization|nan|
|Genetic: molecular genetic technique|nan|
|Genetic: mutation analysis|nan|
|Genetic: polymerase chain reaction|Not noted|
|Genetic: proteomic profiling|nan|
|Other: immunohistochemistry staining method|not noted|
|Other: laboratory biomarker analysis|not noted|
|Other: mass spectrometry|not noted|
|Other: medical chart review|not noted|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Identification of proteomic profiles and molecular pathways involved in tumor progression | Genomic analysis, targeted gene mutation analysis, immunohistochemistry, and mass spectrometry will be employed to identify proteomic profiles and specific molecular pathways involved in tumor progression of fibrolamellar carcinoma and hepatocellular carcinoma | After collection of tissue samples | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Association between fibrolamellar carcinoma and hepatocellular carcinoma in terms of molecular aberrations and clinicopathologic features | Compare and contrast fibrolamellar carcinoma with hepatocellular carcinoma in terms of the molecular differences, tissue pathologies, and medical histories. | After molecular analysis of tissue and after collection of clinicopathologic data | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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childhood hepatocellular carcinoma, childhood liver cancer, adult primary liver cancer, adult primary hepatocellular carcinoma