Leucine and Beta-hydroxy-beta-methylbutyrate (HMB) as Anabolic Nutrients After 36 Hours of Fasting

Study Overview
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Brief Summary
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The purpose of this study is to investigate metabolic effects of 3 different nutritional compounds. Fasting (36 hours) creates a catabolic state that is comparable with the catabolic state seen in acute illness. This study is a randomized, placebo cross-over study investigating 8 healthy men on 4 separated days (at least 3 weeks between trials). High leucine content drink Low leucine content drink Low leucine content drink + HMB Carbohydrate (isocaloric) The investigators hypothesize that these nutritional supplements may counteract catabolic processes in different ways and degrees.

Official Title
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Leucine and Beta-hydroxy-beta-methylbutyrate (HMB) as Anabolic Nutrients After 36 Hours of Fasting

Conditions
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Fasting, Metabolism, Nutrition Support

Intervention / Treatment
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* Dietary Supplement: low leucine content drink
* Dietary Supplement: high leucine content drink
* Dietary Supplement: low leucine + HMB drink
* Dietary Supplement: Control/carbohydrate
* Behavioral: 36 hour fasting


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: male gender 20<BMI<30 Age > 20 years old Written consent before starting the study Exclusion Criteria: Allergy for soya products ore egg Diabetes Epilepsy Infection Immune defects Heart disease Dysregulated hypertension Participating in other trials using radioactive tracers or x-rays the last year.

Ages Eligible for Study
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Minimum Age: 20 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: control<br>Isocaloric carbohydrate drink | Dietary Supplement: Control/carbohydrate<br> <br> Behavioral: 36 hour fasting<br> <br> |
| Active Comparator: low leucine content drink<br>A soy protein drink | Dietary Supplement: low leucine content drink<br> <br> Behavioral: 36 hour fasting<br> <br> |
| Active Comparator: high content of leucine drink<br>A whey protein drink | Dietary Supplement: high leucine content drink<br> <br> Behavioral: 36 hour fasting<br> <br> |
| Active Comparator: low leucine content + HMB drink<br>Soy protein drink added HMB | Dietary Supplement: low leucine + HMB drink<br> <br> Behavioral: 36 hour fasting<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Protein metabolism after 36 hours fasting receiving different nutritional supplements. | Protein metabolism is measured with amino acid tracer techniques comparing marked amino acids from blood samples. Muscle and fat biopsies are obtained for signaling analysis. Indirect calorimetry measurements are used to compare metabolism in the control period and during the intervention. | 3 control period followed by 4 hours of nutritional drink intake |