The Bioavailability of Polyacetylenes From Carrots Study

Study Overview
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Brief Summary
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Polyacetylenes, compounds found naturally in carrots, have shown promising anti-cancer and anti-inflammatory actions in vitro but have not been tested in vivo. To determine whether the polyacetylenes could have an effect in vivo, this study aims to determine whether they are detectible in human biofluids after the consumption of a portion of carrots and whether a difference is seen between a large and small portion.

Detailed Description
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Volunteers will collect urine samples 24 hours prior to the 'test' day and a stool sample either the night before or the morning of the test session. They will have fasted blood taken before eating a portion of carrots with bread and butter. Further blood samples will be taken at 0.5, 1, 2, 3, 4, 6, 8 and 24 hours. All urine and all stool samples (after 4 hours) will be collected up to 48 hours after the carrot dose. They volunteers will attend two test sessions and will be randomised to receive high and low doses of carrot. At least a week will separate testing sessions.

Official Title
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Absorption of Naturally Present Compounds in Carrots and Their Appearance in Blood and Other Biofluids After Consumption (The Carrot Study)

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Carrot


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy adults, BMI between 18.5 and 30 Exclusion Criteria: Metabolic or gastrointestinal conditions, allergies to carrot or bread, taking any medication that affects metabolism or digestion, smokers

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: High Carrot Dose<br>The volunteer will eat 250g carrot for breakfast with 2 slices of bread and 10g butter | Dietary Supplement: Carrot<br> <br> * Other names: falcarindiol-3-acetate;|
| Experimental: Low Carrot Dose<br>The volunteer will eat 100g of carrot for breakfast with 2 slices of bread and 10g butter | Dietary Supplement: Carrot<br> <br> * Other names: falcarindiol-3-acetate;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Detection of polyacetylene in biofluids | The primary outcome will be whether a change in concentration of polyacetylene is seen between baseline and various time points in the biofluids after eating carrots. | 72 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Detection of a difference between doses of polyacetylene in biofluids | The secondary outcome will be whether there is a difference between the high and low dose concentrations of polyacetylenes at various time points after eating carrots. | 72 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Carrot, Bioavailability, Polyacetylenes, Falcarinol, Falcarindiol, Falcarindiol-3-acetate, Biological Availability