Hemophilia and Bone Metabolism: Study of Monocytic Populations and Inflammatory Proteins

Study Overview
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Brief Summary
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The investigators propose, as part of the study, to carry out for each patient: An analysis of monocytic populations by flow cytometry (CD14, CD16, CD45, CD68, CD115, CCR2, CX3CR1, CD163 and CD206). A population assessment of Myeloid-Derived Suppressor Cells (MDSC). Assays of cytokines and chemokines involved in inflammation by multiplex analyzes: Il-1 (α and β), Il-4, Il-6, Il-10, Il-13, TNF- α, TGF- β, CRP , leptin, IFN- β. Specialized dosages of proteins involved in bone metabolism. RANKL, osteoprotegerin, M-CSF, TRAPCP5.

Official Title
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Hemophilia and Bone Metabolism: Study of Monocytic Populations and Inflammatory Proteins

Conditions
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Hemophilia, Osteopenia, Arthropathy

Intervention / Treatment
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* Other: Observational study


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Major patients followed at the hemophilia treatment reference center, Presenting the diagnosis of severe, moderate or minor Hemophilia A / B, Having a social security system, Obtaining the oral non-objection collection from the patient after information. Exclusion Criteria: Minor patients, under guardianship or curatorship. Causes of secondary osteoporosis: Systemic corticosteroid therapy in progress (≥ 3 consecutive months, at a dosage ≥ 7.5 mg / d of prednisone equivalent). other treatment or condition responsible for osteoporosis: prolonged or medicated hypogonadism, untreated active hyperthyroidism, hypercorticism, primary hyperparathyroidism. Treatment with biphosphonates, biotherapies or hormone therapy in the treatment of prostate cancer.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: Observational study|Observational study|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To study the monocytic populations possibly involved in bone metabolism in hemophiliac patients. | Evaluation of the inflammatory profile of patients by protein assays | 1 year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Find a correlation between the main objective and the measurement of bone density | Evaluation of biochemical parameters involved in bone metabolism | 1 year | 
| Find a correlation between the main objective and the measurement of bone density | Non-invasive bone mineral density measurement | 1 year | 
| Look for a correlation between the main objective and physical activity | Assessment of physical activity by self-questionnaire | 1 year | 
| Look for a correlation between the main objective and the inflammatory profile in hemophiliac patients. | Evaluation of the inflammatory profile of patients by protein assays | 1 year |