Hop Compounds and Immune System

Study Overview
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Brief Summary
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The aim of the present study is to determine the effect of Xanthohumol and Iso-alpha-acids from hops on the immune response of healthy participants.

Detailed Description
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In this placebo-controlled crossover study a study drink enriched with either a placebo, Xanthohumol, Iso-alpha Acids or a combination of latter is given to the participants. A washout phase before intervention and between each intervention is introduced. On day one of each intervention blood is drawn in fasted state from each participant. Subsequently the study drink combined with a standardized breakfast is given to the participants following a second blood drawing one hour after the consumption of the study drink. On the last day of each intervention blood is taken from study participants in the same manner as on day one. To assess the effect of Xanthohumol and Iso-alpha acids or a combination of both, clinical chemical parameters e.g. ALT, AST, γ-GT, blood lipids (triglycerides, total cholesterol, HDL, LDL), blood glucose, uric acid and CRP are determined. Furthermore, PBMC's isolated from blood samples of each time point were stimulated for either 24 or 48 h. Protein levels and mRNA expression of markers of inflammation is measured in PBMC's and supernatant of stimulated cells.

Official Title
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Effect of Iso-alpha-Acids and Xanthohumol on Inflammatory Processes in Human

Conditions
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Inflammatory Response

Intervention / Treatment
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* Other: Dietary Intervention 1: Placebo
* Other: Dietary Intervention 2: Xanthohumol and Iso-alpha acids


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy, normal weight, Exclusion Criteria: food intolerances, food allergies, chronic inflammatory diseases, metabolic diseases, viral or bacterial infections within the last 3 weeks of inclusion, intake of immunosupressive medication

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Placebo<br>Participants receive a study drink supplemented with a placebo over a time period of 5 days. | Other: Dietary Intervention 1: Placebo<br>* Participants receive a study drink supplemented with a placebo for 5 days<br>|
| Experimental: Xanthohumol<br>Participants receive a study drink supplemented with Xanthohumol over a time period of 5 days. | Other: Dietary Intervention 2: Xanthohumol and Iso-alpha acids<br>* Participants receive a study drink supplemented with xanthohumol, Iso-alpha acids or a combination of both for 5 days<br>|
| Experimental: Iso-alpha acids<br>Participants receive a study drink supplemented with Iso-alpha acids over a time period of 5 days. | Other: Dietary Intervention 2: Xanthohumol and Iso-alpha acids<br>* Participants receive a study drink supplemented with xanthohumol, Iso-alpha acids or a combination of both for 5 days<br>|
| Experimental: Xanthohumol/Iso-alpha acids<br>Participants receive a study drink supplemented with a combination of Xanthohumol and Iso-alpha acids over a time period of 5 days. | Other: Dietary Intervention 2: Xanthohumol and Iso-alpha acids<br>* Participants receive a study drink supplemented with xanthohumol, Iso-alpha acids or a combination of both for 5 days<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in parameters of immune response | Parameters of immune response are measured in cell culture supernatant of stimulated cells | 5 days |