Investigating the Consumption of Different Protein Rich Whole Food Sources on Muscle Growth in Trained Males and Females

Study Overview
=================
Brief Summary
-----------------
Background To date, most of the literature investigating the role of dietary protein in muscle growth has focused on isolated protein sources. However, dietary protein is most commonly consumed within a whole food source. Consuming dietary protein within a whole food matrix may additionally stimulate muscle growth. The idea being, there may be other components within food, capable of producing a greater response. Objectives: To assess the effect of consuming a variety of whole food sources, on the stimulation of muscle growth compared to an isolated protein source following a single bout of lower body resistance exercise. Methods Young healthy resistance-trained volunteers will consume a protein rich whole food source, following a bout of lower body resistance exercise. Stable isotope IV infusions and repeated blood and muscle samples will be taken to assess protein digestion and absorption, as well as muscle protein synthesis rates. Value of Research: To determine whether whole food sources potentiate muscle protein synthesis rates (and to what extent across whole food sources) compared to an isolated protein source. In doing so, we can further investigate the nutrients involved that may be contributing to this effect.

Official Title
-----------------
Assessing the Muscle Protein Synthetic Response Following the Ingestion of a Variety of Protein Rich Whole Foods Compared to an Isolated Protein Source.

Conditions
-----------------
Nutritional Intervention Using Protein Rich Whole Food Sources

Intervention / Treatment
-----------------
* Dietary Supplement: whole food sources


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Males and Females BMI 18.5 - 30 kg/m2 Aged 18 - 40 years Structured Resistance training consistently for > 6 months A 5-7 day washout period from any supplementation Exclusion Criteria: Age >40 years Body mass index (BMI) <18.5 or >30 kg/m2 Any metabolic impairments Any cardiovascular impairments High blood pressure (≥140/90 mmHg) Any gastrointestinal disorders Any medications known to affect protein and/or amino acid metabolism A personal or family history of epilepsy, seizures or schizophrenia, motor disorder Chronic over the counter use of pharmaceuticals (> 1 month) Allergic to any of the whole foods included in the study

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Pork<br>Locally sourced | Dietary Supplement: whole food sources<br>* Foods selected are naturally high in protein and consumed by the general population as part of a meal.<br>|
| Experimental: Salmon<br>Locally sourced | Dietary Supplement: whole food sources<br>* Foods selected are naturally high in protein and consumed by the general population as part of a meal.<br>|
| Experimental: Mycoprotein<br>Provided by Marlow Foods | Dietary Supplement: whole food sources<br>* Foods selected are naturally high in protein and consumed by the general population as part of a meal.<br>|
| Experimental: Lentils<br>Red | Dietary Supplement: whole food sources<br>* Foods selected are naturally high in protein and consumed by the general population as part of a meal.<br>|
| Experimental: Egg<br>Local supplier | Dietary Supplement: whole food sources<br>* Foods selected are naturally high in protein and consumed by the general population as part of a meal.<br>|
| Active Comparator: Egg whites<br>Local Supplier | Dietary Supplement: whole food sources<br>* Foods selected are naturally high in protein and consumed by the general population as part of a meal.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Postprandial Muscle protein Synthetic Response following the ingestion of a protein rich whole food source. | 84 participants will undergo a single bout of lower body exercise followed by the ingestion of one of the six protein rich whole food sources. Using stable isotope tracer methodology and muscle biopsy samples, the postprandial muscle protein synthetic response will be able to be calculated. | 2 years |