The Effect of Alginate on Carbohydrate and Fat Digestion in a Mixed Meal

Study Overview
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Brief Summary
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This study will investigate the effect of an extract of brown seaweed, called alginate, on fat digestion. The extract has been cooked into sausages and the alginate sausages will be compared to the same sausages that do not contain alginate. People taking part in the study will come to the study centre twice. Once to have the alginate sausage meal or the normal sausage meal and on the second visit they will have the other meal so then end up having had both types of sausages. The level of fat in their blood will be monitored over the four hours after the meal. The rate and amount of fat that moves into the blood after a meal is an indication of the amount of fat digestion. We believe that less fat will be digested and move into the blood when the volunteers eat the alginate sausages compared to the normal sausages.

Detailed Description
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Alginate is an extract of brown seaweed and has been shown to reduce lipase activity in vitro. Lipase is a valid target for anti-obesity products as shown by the pharmaceutical drug orlistat which targets the same enzyme. Reducing the amount of lipase activity will reduce the amount of triacylglycerol (TAG) that will be digested and therefore absorbed and will be seen as a reduction in the level of circulating TAG in the blood. Adding alginate to a range of food products that are simple to include into the diet could help with the management of obesity. In this study, sausages containing alginate have been manufactured for us along with matched control sausage. The circulating TAG in the blood will be measured via fingerpick blood samples every 30 minutes for four hours after the volunteers have eaten either alginate sausages or normal sausages. The volunteers will come for two visits and consume the other sausage meal at the second so they will have had both types of sausage.

Official Title
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The Effect of Alginate on Carbohydrate and Fat Digestion in a Mixed Meal - A Pilot Trial

Conditions
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Triacylglycerol Digestion

Intervention / Treatment
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* Dietary Supplement: Alginate Sausage
* Other: Control Sausage


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Exclusion Criteria: pregnant or lactating.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Double blind randomised controlled trial
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Alginate then Control<br>This group will be given the alginate then the control sausages | Dietary Supplement: Alginate Sausage<br>* Alginate sausage<br>Other: Control Sausage<br>* Control Sausage<br>|
| Experimental: Control then Alginate<br>This group will be given the control then alginate sausages | Dietary Supplement: Alginate Sausage<br>* Alginate sausage<br>Other: Control Sausage<br>* Control Sausage<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Concentration of circulating TAG in blood | mmol/L | 4 hours after the test meal | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Concentration of circulating glucose in blood | mmol/L | 4 hours after the test meal | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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fat digestion, alginate, sausages