Casein Phosphopeptide--amorphous Calcium Phosphate on Human Enamel Subjected to in Vivo Acid Attacks

Study Overview
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Brief Summary
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40 specimens were prepared from fresh-extracted human molars and further embedded using the acrylic resin. Specimens were randomly divided into 2 groups according to the treatment before in vivo erosion (n=20): CPP-ACP for 3 min (CPP-ACP group) and deionized water for 3 min (control group). 10 healthy volunteers were recruited and customized maxillary appliance containing 4 specimens (2 from CPP-ACP group and 2 from control group) was fabricated for each volunteer. Participants were instructed to drink 150 ml cola in 5 min using the gargling method after placing appliances intraorally for 2 h. The in vivo attacks were performed 4 X 5 min with 1-h interval daily over 7 d. The surface microhardness and surface microstructure of the specimens were evaluated at the baseline and after the treatment. The data were statistically analyzed using three-way ANOVA and post hoc tests. Data were considered statistically significant at a level of P <0.05.

Official Title
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In Situ Effect of Casein Phosphopeptide--amorphous Calcium Phosphate on the Surface Microhardness of Human Enamel Subjected to in Vivo Acid Attacks

Conditions
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Dental Erosion

Intervention / Treatment
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* Drug: CPP-ACP
* Drug: Placebos


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 to 60 years of age and in good general health Dental erosion and wear degree is 0-1 Exclusion Criteria: Systemic diseases or oral mucosal disorders Current orthodontic treatment Pregnancy Known allergies to the experimental drink Had been given drugs which lead to dry oral more regularly within half a year

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: CPP-ACP<br>Tooth mousse (GC, Japan) application on the specimen surface for 3 min. | Drug: CPP-ACP<br>* The surfaces of specimens were pretreated with CPP-ACP for 3 min<br>|
| Placebo Comparator: Deionized water<br>Deionized water application on the specimen surface for 3 min | Drug: Placebos<br>* The surfaces of specimens were pretreated with deionized water for 3 min<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Surface microhardness | a Vickers microhardness tester with microscopic lens was used, with a 100 g load and 30-s dwell time. Three microhardness measurements were obtained on the top surface of each specimen. | baseline | 
| Surface microhardness | a Vickers microhardness tester with microscopic lens was used, with a 100 g load and 30-s dwell time. Three microhardness measurements were obtained on the top surface of each specimen. | 7 days |