Endometriosis Impact on Oocyte Quality

Study Overview
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Brief Summary
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The aim of this study is to evaluate the impact of endometriosis on folliculogenesis and oocyte quality. To do so, a metabolomic approach will be conducted in order to analyze the follicular fluid. This evaluation will be completed by a transcriptomic analysis from the cumulus cells of the oocyte. The normal and pathological oocyte cohort after controlled ovarian stimulation will be also characterized by identifying the oocyte leading to live birth.

Detailed Description
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Endometriosis pathophysiology remains under controversy. Among the various issues raised, that of his involvement in an implantation failure related to an alteration of the endometrium is advanced by some authors. For others, infertility would be linked to an alteration of the oocyte quality responsible for embryonic development impairment leading to a lack of implantation. Several research groups have also mentioned the association of the two mechanisms. The oocyte quality evaluation is also subject to controversy. Indeed, its morphological approach remains the most commonly used in routine at the IVF laboratory. However, this tool remains limited and to date, no correlation between oocyte morphology and ART outcomes have been established. In this context, there is a real need to use functional approaches such as genomics, transcriptomics, proteomics and metabolomics. However, the clinical validation and application of these functional tools have to be evaluated from a human pathology model such as endometriosis.

Official Title
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Characterization of Normal and Pathological Oocyte Cohort After Controlled Ovarian Stimulation: Example of Endometriosis

Conditions
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Endometriosis

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Group 1: case a. Womens with endometriosis i. Deep endometriosis without ovarian localisation ii. Deep endometriosis associated with ovarian localisation iii. Isolated ovarian localisation b. Male partner with normal sperm parameters Group 2 : control Womens with isolated tubal factor Male partner with normal sperm parameters Exclusion Criteria: Other pathologies responsible for female infertility Altered sperm parameters Male or female viral risk Oocyte or sperm donation

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Case : endometriosis<br>Infertile patients with endometriosis that is the only cause of their infertility | |
| Control : tubal factor<br>infertile patients with tubal factor that is the only cause of their infertility | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolic profile | Identification of a possible particular metabolomic profile from the follicular fluid in endometriosis | 2 years | 
| Transcriptomic profile | Identification of a possible particular transcriptomic profile from the cumulus cells in endometriosis | 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oocyte characterization | Identification of a possible transcriptomic profile from the cumulus cells of the oocyte which could be predictive of live birth. Search of correlations between these profiles and (i) oocyte and embryo morphology and (ii) clinical and neonatal outcomes after embryo transfer. | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Endometriosis, Metabolomics, Transcriptomics, Follicular fluid, Cumulus cells, Oocyte quality