A Project to Study Probiotic Beauty Products on Skin Improvement

Study Overview
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Brief Summary
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The aim of the study was to use probiotic beauty products for 4 weeks and evaluate the impact of products on healthy skin.

Detailed Description
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Twenty subjects were recruited and informed to apply the lotion gel twice per day (3 5 mg/cm2 once) for 4 weeks. Two kinds of facial lotiongels, A gel and B gel, were provided to every subject. The A gel (TAC/Collagen lotiongel) was applied on subjects' right face and the B gel (placebo lotion) on their left face (formula showed as Table S2). Ordinary cosmetics usage was permitted but any change of usage was not allowed during the intervention. Outdoor activities (sun exposure) and the usage of sunscreen lotion were not limited in this study. Every subject was required to inspect his/her skin condition on week 0 and 4. Before inspection, the face was washed with water and waited for 30 min in an air-conditioned room (25°C, humility 55 ± 5%). Among these 20 subjects, eight of them had severe acne with at least two acne lesions on each side of face when participating this trial. They were instructed for more visits to evaluate the symptom of acne on week 1 and 2. Skin hydration (Corneometer CM825, Courage + Khazaka Electroni, Germany), sebum (Callegari 1930, Italy), and inflammation detection (Chroma Meter MM-500, Minolta, Japan) of upper cheek were measured in every subject. The improvement in inflammation was positively correlated with a decrease of a* value or an increase of L* value. VISIA® Complexion Analysis (VISIA® Complexion Analysis, U.S.A.) was also employed to measure the skin inflammation, porphyrins numbers and brown spot numbers of whole face. The result was presented as the mean value and the relative percentage (%) to the baseline.

Official Title
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Chia Nan University of Pharmacy & Science

Conditions
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Skin Diseases

Intervention / Treatment
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* Dietary Supplement: TAC/Collagen gel
* Dietary Supplement: placebo lotion


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy volunteers aged above 20 years old Exclusion Criteria: Subject with diseases of the skin, heart, liver, kidney, endocrine and other organs and patients with mental illness (according to medical history). Subjects who have known cosmetic, drug or food allergies, difficulty in digestive tract absorption or disorder. Female who is pregnant or nursing or planning to become pregnant during the course of the study. Received facial laser therapy, chemical peeling or UV overexposure in the past 4 weeks. Involuntary subject People who is taking chronic disease medication

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: A gel (TAC/Collagen gel)<br>The A gel (TAC/Collagen gel) was applied on subjects' right face | Dietary Supplement: TAC/Collagen gel<br>* Apply it twice per day for 4 weeks<br>|
| Placebo Comparator: B gel (placebo lotion)<br>the B gel (placebo lotion) on their left face | Dietary Supplement: placebo lotion<br>* Apply it twice per day for 4 weeks<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Effects of the TAC/Collagen Gel on Human Skin Hydration | Corneometer® CM825 was utilized to measure skin moisture. Units: arbitrary Corneometer® units 0-120 | Subjects will be tested in the 4th week | 
| Effects of the TAC/Collagen Gel on Human Skin Sebum | Callegari 1930 was utilized to measure skin inflammation. Units: arbitrary units. | Subjects will be tested in the 4th week | 
| Effects of the TAC/Collagen gel on Human Skin Inflammation | Chroma Meter MM-500 was utilized to measure skin inflammation. Units: arbitrary units. | Subjects will be tested in the 4th week | 
| Effect of TAC/Collagen Gel on Human Acne Healing and Porphyrins Numbers | VISIA Complexion Analysis System was utilized to measure skin texture. Units: arbitrary units | Subjects will be tested in the 4th week | 
| Effects of the TAC/Collagen Gel on Human Skin Brown Spot Numbers | VISIA Complexion Analysis System was utilized to measure skin texture. Units: arbitrary units | Subjects will be tested in the 4th week |