Intestinal Parasites in Inhabitants of Kuyavian-Pomeranian Province in Poland

Study Overview
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Brief Summary
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The goal of the proposed study is to assess the prevalence of intestinal parasitic diseases in adults and children. These invasions are included to dirty hand diseases (per os invasions), therefore it applies mainly preschool children.

Detailed Description
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It is estimated that 75% of the world's population is infected with parasites and although parasitic diseases are mostly observed in the poor tropical and subtropical countries, there are also many diseases caused by parasites in Poland. In Poland, particular attention is paid to the following parasitic diseases closely related to the digestive system: giardiasis, taeniasis (including cysticercosis), ascariasis, cryptosporidiosis, toxoplasmosis, trichinosis, echinococcosis. Symptoms of such invasions may include: allergic rhinitis of unknown origin, chronic fatigue or food intolerance. Moreover, many of them are asymptotic, therefore the study group will consist of both healthy people and individuals with undiagnosed diseases or/and chronic symptoms.

Official Title
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Diagnostics of Intestinal Parasitosis in Inhabitants of Kuyavian-Pomeranian Province in Poland

Conditions
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Intestinal Disease, Parasitic, Chronic Disease

Intervention / Treatment
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* Diagnostic Test: Tests for diagnosis of intestinal parasitic diseases


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy and ailing individuals men and females adults and children Exclusion Criteria: the participants were not be: incapacitated persons, soldiers, prisoners and persons dependent in any way from the investigators

Ages Eligible for Study
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Minimum Age: 3 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Study participants<br>Both healthy and ailing individuals, both sex, including adults and children. | Diagnostic Test: Tests for diagnosis of intestinal parasitic diseases<br>* Feces testing: fresh fecal samples will be used to preparing direct (saline and iodine wet) mounts using supplementary techniques (flotation, sedimentation), as well as temporary mounts (hematoxylin and trichrome staining). Other technique of fresh fecal samples examination will be ParaSys™ A non-preserved stool samples will be stored below -20°C. Defrosted fecal specimen will be subjected to enzyme-linked immunosorbent assays (ELISA) - Giardia antigen test (coproantigens)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Intestinal parasites | The fecal samples will be assessed qualitavely in terms of life forms of parasites present in human intestine, i.e.: eggs and larvas of flukes, tapeworms and nematodes, as well as protozoa trophozoites and cysts. | 1 day (single evaluation of current samples) | 
| Giardia antigen test | Stool test for Giardia lamblia antigen (ELISA kit) | 1 day (single evalutaion of all samples through study completion) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Per os invasions, Giardiasis, Toxoplasmosis, Taeniasis, Cryptosporidiosis, Trichinosis, Ascariasis, Echinococcosis