Evaluation of Mitochondrial Dysfunction in Severe Burn and Trauma Patients

Study Overview
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Brief Summary
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The purpose of this project is to evaluate the level of mitochondrial dysfunction several patient populations: Burn, trauma, and control group of healthy volunteers. Study hypothesis: Increased plasma concentrations of a newly discovered inflammatory mediated, called mtDNA DAMPS associated with the occurrence of multi-organ dysfunction syndrome in severly injured patients. As the severity of a burn injury or trauma injury increase, so will systemic mitochondrial dysfunction.

Detailed Description
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Increased plasma concentrations of a newly discovered inflammatory mediated, called mtDNA DAMPS associated with the occurrence of multi-organ dysfunction syndrome in severly injured patients. A normal healthy sub-population will be used as a control.

Official Title
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Evaluation of Mitochondrial Dysfunction in Severe Burn and Trauma Patients

Conditions
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Burns, Trauma

Intervention / Treatment
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* Other: trauma: blood collection
* Other: healthy volunteers: blood collection
* Other: Burn: blood collection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Burn injury must be less than 24 hours old at time of initial sampling Burn must be 2nd or 3rd degree and at least 10% TBSA ISS (injury severity score) > 15 Exclusion Criteria: Burn injury/trauma injury > than 24hours old at time of initial sampling Burn < 10% total body surface area. Evidence of active infection on admission in the burn subjects. < 19 years of age and > 70 years

Ages Eligible for Study
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Minimum Age: 19 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Factorial Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Burn: blood collection<br>Procedure: Blood draws as the intervention. | Other: Burn: blood collection<br>* blood collected at designated time intervals<br>|
| Other: trauma: blood collection<br>blood to be collected at different time intervals. Blood draw as the intervention | Other: trauma: blood collection<br>* Blood samples collected at certain timepoints. Time points for burn/trauma subjects: Day0,Day1,Day2,and Day6 and Day7.<br>|
| Other: healthy volunteers: blood collection<br>Blood draws as the intervention | Other: healthy volunteers: blood collection<br>* blood collected at designated time intervals<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma mtDNA DAMPs concentration | Plasma mtDNA DAMPs concentration | approximately 1 year to assess outcome measure | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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burns, trauma