Study of Pomegranate Juice in the Modulation of Oxidative Stress Markers in Endurance-based Athletes

Study Overview
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Brief Summary
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The purpose of this study is to evaluate modifications of plasma protein carbonyls and malondialdehyde levels in endurance-based athletes after three weeks of pomegranate juice consumption.

Conditions
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Oxidative Stress

Intervention / Treatment
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* Dietary Supplement: Pomegranate juice


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Perform specific endurance training sessions More than 1 hour per session and more than 3 sessions per week Have participated recently in a half marathon or similar event Exclusion Criteria: Intake of antioxidant or anti-inflammatory supplementation Present a chronic disorder Non-smoker No-alcoholic beverage consumption

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: Control<br>Arm that followed all the protocols with the exception of the pomegranate juice intake. | |
| Experimental: Pomegranate Juice<br>Arm that followed all the protocols and the pomegranate juice intake. | Dietary Supplement: Pomegranate juice<br>* Intake of 200 ml of pomegranate juice or pomegranate juice dilution during 21 days after exercise.<br>* Other names: Zumo de Granada Vitalgrana;|
| Experimental: Pomegranate Juice Dilution<br>Arm that followed all the protocols and the pomegranate juice dilution (1:1) intake. | Dietary Supplement: Pomegranate juice<br>* Intake of 200 ml of pomegranate juice or pomegranate juice dilution during 21 days after exercise.<br>* Other names: Zumo de Granada Vitalgrana;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Protein carbonyls levels changes in plasma | Protein carbonyl derivatives were determined using an adaptation of the colorimetric method published in Levine et al. (1994) based in the formation of a stable 2,4-dinitrophenyl (DNP) hydrazone product after the addition of 2,4-dinitrophenylhydrazine (DNPH) to tha plasma. Protein carbonyls were calculated as nmoles·mg-1 protein | day 0 and 22 | 
| Malondialdehyde levels changes in plasma | Malondialdehyde was determined by high pressure liquid chromatography (HPLC) with fluorescence detection according to the method described by Laporta et al. (2007). Malondialdehyde levels were calculated as nmoles·g-1 protein | day 0 and 22 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood biochemical parameters changes in plasma | Blood biochemical parameters is a composite outcome measure consisting in circulating plasma glucose (mg·dL-1), HDL-cholesterol (mg·dL-1), triglycerides (mg·dL-1), ferritin (ng·mL-1), lactate (mg·dL-1), sodium (mEq·L-1), potassium (mEq·L-1), creatine kinase (U·L-1), aspartate aminotransferase (U·L-1) and alanine aminotransferase (U·L-1) were analyzed through biochemistry methods. | day 0 and 22 | 
| C-reactive protein levels changes in plasma | Determination by immunoassay of C-reactive protein (ng·mL-1) levels in plasma. | day 0 and 22 | 
| Soluble E-selectin levels changes in plasma | Determination by immunoassay of soluble E-selectin (ng·mL-1) levels in plasma. | day 0 and 22 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Antioxidants, Protein Carbonylation, Malondialdehyde, Punicaceae, Athletic Performance