Orange Juice Consumption and Glucose Metabolism and Antioxidative Status

Study Overview
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Brief Summary
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Aim of the study is to investigate the impact of orange juice consumption (with meals or between meals) as well as sugar sweetened beverage (SSB) consumption (between meals) on glucose homeostasis and antioxidative status.

Detailed Description
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Duration of the study is 3x three weeks including one wash-out week each followed by two weeks of juice/SSB consumption under free living conditions. During wash-out weeks consumption of orange juice or citrus fruits is restricted. During intervention weeks, participants are asked to eat three regular meals per day without snacking. Intervention weeks are (i) orange juice consumption with meals (ii) orange juice consumption between meals and (iii) SSB consumption between meals. Daily energy intake with orange juice/SSB is 20% of individual energy requirement. Additional caloric beverages (SSB, juice, coffee with sugar/milk, alcohol) are not allowed. At the beginning and the end of each intervention phase insulin sensitivity and antioxidative status are assessed.

Official Title
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Impact of Orange Juice Consumption on Glucose Homeostasis and Antioxidative Status in Healthy Subjects

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Orange juice with meals
* Dietary Supplement: Orange juice between meals
* Dietary Supplement: Sugar sweetened beverage between meals


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy Exclusion Criteria: regular use of medication, smoking, chronic disease, vegan/vegetarian diet, fructose intolerance, irregular eating behaviour (less then 3 meals/day)

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Orange juice with meals<br>Effect of orange juice consumption with three meals per day (without snacking) on glucose metabolism and antioxidative status | Dietary Supplement: Orange juice with meals<br>* orange juice (20% of energy requirement/day)<br>|
| Experimental: Orange juice between meals<br>Effect of orange juice consumption between three meals per day (without snacking) on glucose metabolism and antioxidative status | Dietary Supplement: Orange juice between meals<br>* orange juice (20% of energy requirement/day)<br>|
| Experimental: Sugar sweetened beverage between meals<br>Effect of sugar sweetened beverage consumption between three meals per day (without snacking) on glucose metabolism and antioxidative status | Dietary Supplement: Sugar sweetened beverage between meals<br>* Sugar sweetened beverage (20% of energy requirement/day)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| changes in daylong glycaemia between pre and post intervention periods | measured via continuous glucose monitoring [mg/dL] | two weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| changes in uric acid between pre and post intervention periods | measured via uric acid serum levels [mg/dL] | two weeks | 
| changes in insulin sensitivity between pre and post intervention periods | measured via Matsuda-Index from oral glucose tolerance test data | two weeks | 
| changes in antioxidative status between pre and post intervention periods | measured via vitamin c blood levels [mg/L] | two weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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orange juice consumption, glucose homeostasis, antioxidative status