Immune Characterization of Subcutaneous Adipose Tissue

Study Overview
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Brief Summary
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The overall goal of this proposal is to characterize the immune cell profile of subcutaneous abdominal adipose tissue in lean, overweight, obese and lipedema adults, and to evaluate potential associations between different adipose tissue immune cell types and metabolic profile.

Official Title
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Immune Characterization of Subcutaneous Adipose Tissue

Conditions
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Determine Immune Cell Types in Subcutaneous Adipose Tissue

Intervention / Treatment
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* Procedure: Liposuction/lipectomy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Women of all races and ethnicity Age between 18 and 65 years Body Mass Index (BMI) ≥ 18.5 kg/m² Weight stable (+/- 3% during the last 2 months before enrollment) Women already scheduled for above mentioned procedures Exclusion Criteria: Diabetes Mellitus Recent (<5 years) history of cancer (except in situ ductal breast carcinoma) Chemotherapy and/or radiation therapy within 4 months before enrollment Immune system diseases (i.e., autoimmune disorders, acquired immunodeficiency syndrome) Pregnant or breastfeeding Take any medication that might affect immune function Severe organ dysfunction BMI <18.5 kg/m² or size of each breast is >600 grams, if scheduled for bilateral breast reconstruction

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Normal Weight (BMI: 18.5-24.9 kg/m²)<br>Liposuction/lipectomy | Procedure: Liposuction/lipectomy<br>* Subcutaneous adipose tissue will be obtained from liposuction/lipectomy to determine the types of immune cells<br>|
| Overweight (BMI: 25.0-29.9 kg/m²)<br>Liposuction/lipectomy | Procedure: Liposuction/lipectomy<br>* Subcutaneous adipose tissue will be obtained from liposuction/lipectomy to determine the types of immune cells<br>|
| Obese (BMI: >30.0kg/m²)<br>Liposuction/lipectomy | Procedure: Liposuction/lipectomy<br>* Subcutaneous adipose tissue will be obtained from liposuction/lipectomy to determine the types of immune cells<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determine the types of immune cells and the relative prevalence of each type and sub-type, in subcutaneous adipose tissue of normal weight, overweight, obese and lipedema adults. |  | intraoperative | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determine the relationships among specific types and subtypes of immune cells, adiposity and cardiometabolic risk. |  | Baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Subcutaneous adipose tissue, Lipectomy