The Transcriptomic Study of Thai Patients With Atopic Dermatitis by Tape Strips

Study Overview
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Brief Summary
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This study will be use the tape strip technique to evaluate the skin biomarkers of atopic dermatitis among Thai patients to differentiate clinical phenotype.

Detailed Description
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The patients will be enrolled in this study if they have been diagnosed with atopic dermatitis. All participants (AD patients and controls) will be evaluated their skin biomarkers by using tape stripping. The tape strips will be applied to the antecubital fossa to collect the epithelial samples. Then RNA was extracted from the tape strips for mRNA profiling to identify the immune and epidermal barrier genes.

Official Title
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The Transcriptomic Study of Thai Patients With Atopic Dermatitis by Tape Strips

Conditions
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Atopic Dermatitis

Intervention / Treatment
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* Genetic: Tape strips


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Children (age 1-18 years old) with mild atopic dermatitis Children (age 1-18 years old) with moderate to severe atopic dermatitis Children (age 1-18 years old) with moderate to severe atopic dermatitis and food allergy Adult (age 18-60 years old) with atopic dermatitis Healthy individuals (1-60 years old) Patients with asthma (1-60 years old) Exclusion Criteria: Active skin infections Used systemic immunosuppressants within 4 weeks Used topical steroids or immunomodulators within 1 week Used moisturizers within 12 hours before evaluation

Ages Eligible for Study
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Minimum Age: 1 Year
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Children with mild atopic dermatitis<br>age 1-8 years old mild degree of atopic dermatitis | Genetic: Tape strips<br>* Tape strip is a minimally invasive method that captures the stratum corneum. It is use to identify skin biomarkers in atopic dermatitis.<br>|
| Active Comparator: Children with moderate-severe atopic dermatitis<br>age 1-8 years old moderate or severe degree of atopic dermatitis | Genetic: Tape strips<br>* Tape strip is a minimally invasive method that captures the stratum corneum. It is use to identify skin biomarkers in atopic dermatitis.<br>|
| Active Comparator: Children with food allergy and moderate-severe atopic dermatitis<br>age 1-8 years old moderate or severe degree of atopic dermatitis IgE mediated food allergy | Genetic: Tape strips<br>* Tape strip is a minimally invasive method that captures the stratum corneum. It is use to identify skin biomarkers in atopic dermatitis.<br>|
| Active Comparator: Adult with atopic dermatitis<br>age 18-60 years old mild-severe atopic dermatitis | Genetic: Tape strips<br>* Tape strip is a minimally invasive method that captures the stratum corneum. It is use to identify skin biomarkers in atopic dermatitis.<br>|
| Active Comparator: Healthy<br>age 1-60 years old no history of atopic diseases | Genetic: Tape strips<br>* Tape strip is a minimally invasive method that captures the stratum corneum. It is use to identify skin biomarkers in atopic dermatitis.<br>|
| Active Comparator: Healthy with Asthma<br>age 1-8 years old doctor diagnosed asthma no history of chronic or chronic relapsing eczema | Genetic: Tape strips<br>* Tape strip is a minimally invasive method that captures the stratum corneum. It is use to identify skin biomarkers in atopic dermatitis.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Cellular AD biomarkers | Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0 | 1 month | 
| Immune biomarkers | Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0 | 1 month | 
| Barrier biomarkers | chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0 | 1 month | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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tape strips, endotype