The Effect of Desfluran On Global Dna Methylation

Study Overview
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Brief Summary
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The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.

Detailed Description
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Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.

Official Title
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The Effect of Desfluran Administered in General Anesthesia On Global Dna Methylation

Conditions
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DNA Damage

Intervention / Treatment
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* Drug: Desflurane


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Ability to understand and willingness to sign an informed consent form Elective lumbar spinal surgery that will take at least 120 minutes, American Society of Anesthesiologists (ASA) physical status I and II Be between the ages of 18 and 65 Exclusion Criteria: Patients with ASA physical status III or IV disease Smoking or alcohol consumption Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders recently received radiation, chemotherapy malignancy blood transfusion in operation use of antioxidant supplements have received general anesthesia in the last three months Patients with known occupational exposures (operating room personnel, chemical plant workers

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| group 1<br>Peripheral venous blood samples will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). DNA will be obtained from blood samples , and global DNA methylation analysis will be done. | Drug: Desflurane<br>* Peripheral venous blood samplesof patients will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). Anesthesia maintenance will be provided with 40% O₂ and minimum alveolar concentration (MAC) in 60% air with 6% desflurane. DNA will be obtained from blood samples with the help of DNA isolation kit, and global DNA methylation analysis will performed by ELISA method.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Global DNA methylation change 5 minutes before and 120 minutes after anesthesia induction in peripheral blood mononuclear cells | The global DNA methylation level will be measured 5 minutes before and 120 minutes after anesthesia induction. | 5 minutes before induction of anesthesia ,120 minutes after the beginning of anesthesia | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Desflurane,, DNA methylation, epigenetics, inhalation anesthetics