Effect of AT-derived miRNA on the Biology and Insulin Sensitivity of Skeletal Muscle in Humans

Study Overview
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Brief Summary
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The purpose of this study is examine the effect of fat tissue-released miRNA on skeletal muscle and if abnormal fat tissue-released miRNA contributes to insulin resistance in obese individuals. This information will be important for our understanding of how the body's sugar metabolism is regulated and why people who are obese become insulin resistant and are more likely to develop type 2 diabetes.

Detailed Description
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Study Objectives: To establish and optimize the methodology for measuring adipose tissue miRNA release. To establish and optimize the methodology for measuring the effect of adipose tissue-released miRNA on skeletal muscle biology and insulin sensitivity. To profile adipose tissue-released miRNA in lean insulin-sensitive and obese insulin-resistant healthy individuals. To examine the effects of adipose tissue-released miRNA from lean insulin-sensitive individuals and obese insulin-resistant individuals on skeletal muscle biology and insulin sensitivity.

Official Title
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Effect of AT-derived miRNA on the Biology and Insulin Sensitivity of Skeletal Muscle in Humans

Conditions
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Type 2 Diabetes Mellitus

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Able to communicate meaningfully with the investigator and legally competent to provide informed written consent 18-65 years of age BMI of 20-25 kg/m2 (lean subjects); 30-35 kg/m2 (obese subjects) Stable body weight (< 3 kg change in the last 8 weeks) homeostatic model assessment (HOMA)-insulin resistance <2.7 if lean; homeostatic model assessment (HOMA)-insulin resistance ≥2.7 if obese Exclusion Criteria: Lactation or pregnancy, current and/or within last 6 months, per participant's report Female subjects postmenopausal Cardiovascular disease (unstable angina, myocardial infarction or coronary revascularization within 6 months) Liver disease (AST or ALT(alanine aminotransferase)>2.5 times the upper limit of normal) Kidney disease (creatinine >1.6 mg/dl) Anemia (hemoglobin <12 g/dl in men, <11 g/dl in women) Thyroid dysfunction (abnormal TSH) HbA1c ≥6.5% Uncontrolled hypertension (systolic BP>160 mmHg, diastolic BP>100 mmHg) History of coagulopathies History (within the last 5 years) or presence of malignancy, (skin cancers, with the exception of melanoma, may be acceptable) Current or history of drug abuse or alcohol abuse (>2 drinks/day) Prior treatment (within last 3 months) with systemic glucocorticoids (>2 weeks), beta-blockers, drugs for weight loss, niacin or fibrates History of HIV, active Hepatitis B or C, or Tuberculosis (participant reported) Smoke > 5 cigarettes per day

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy lean insulin-sensitive individuals<br>This group will be studied by measuring the adipose tissue miRNA release, adipose tissue-released miRNA will be profiled, and these effects will be examined. | |
| Obese insulin-resistant individuals<br>This group will be studied by measuring the effect of adipose tissue-release miRNA on skeletal muscle biology and insulin sensitivity, adipose tissue-released miRNA will be profiled, and these effects will be examined on obese insulin-resistant individuals on skeletal muscle biology and insulin sensitivity. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Levels of miRNA will be measured as well as the adipose tissue specific miRNA. | The effect of miRNA released by adipose tissue from lean insulin-sensitive and from obese insulin-resistant individuals on skeletal muscle biology and insulin signaling. | 4 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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micro-RNA, insulin resistance