Hemodialysis and Erythrocyte Fatty-Acid Status: a Lipidomics Study

Study Overview
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Brief Summary
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Hypotension with potentially serious consequences for organ perfusion is a common complication in extracorporeal procedures such as hemodialysis / hemofiltration. The exact reasons for this are still insufficiently clarified. Probably periinterventional vasorelaxant released substances play a crucial role in these procedures. These substances could be due to contact of blood cells with the Membrane in the dialyzer / hemofilter arise. In this project the hypothesis will be checked, if EETs / DHETs are released by Erythrocytes during hemodialysis and thus act as potential candidate products for the result of hypotonic phases in the Dialysis. We will determine differences in RBC fatty acids profiling in hemodialysis patients before and after dialysis intervention. Furthermore a matched control group of healthy individuals will be profiled. RBC fatty acids profiling will be achieved by using targeted HPLC-MS mass spectrometry. It is believed that during hemodialysis / HLM / CVVH there is an increase in EETs / DHETs in the serum and in the erythrocytes. It is believed that shear forces play an important role in the release of erythrocyte EETs / DHETs.

Detailed Description
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Differences in Erythrocyte Fatty-Acid Status Profiling in: before and after hemodialysis intervention in hemodialysis patients and healthy individuals Venous blood will be collected from each subject in the sitting position

Official Title
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Hemodialysis and Erythrocyte Fatty-Acid Status: a Lipidomics Study

Conditions
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Fatty Acid Metabolism, Erythrocytes, Lipidomics, Hemodialysis

Intervention / Treatment
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* Other: Blood sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria for hemodialysis patients: Patients who have a history of renal failure and undergo hemodialysis / hemofiltration Age over 18 years Ability to consent There is a written consent of the study participant Exclusion Criteria for healthy control group: chronic illness requiring any medication pregnancy limitations regarding functioning in the hypoxic chamber inability to follow simple instructions relevant or severe abnormalities in medical history, physical examination

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Erythrocyte Fatty-Acid Status<br>Erythrocyte Fatty-Acid Status Profiling of HD patients are studied before and after a single HD using LC-MS / MS | Other: Blood sampling<br>* blood sample before and after a single HD<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Coefficient of variation of relative quantities of erythrocyte fatty-acid parameters related to its dynamic during Hemodialysis, using LC-MS / MS. | EETs / DHETs will be analyzed in the erythrocytes and serum of the recovered blood samples. The determination will made in cooperation with a Berliner Biotech company. This involves liquid chromatography with MS coupling (LC-MS / MS) used. The data analysis is carried out by means of suitable statistical methods to perform erythrocyte fatty-acid status profiling. | 4-5 Months |