Pathophysiological Study of Characteristics of the Changes in the Cutaneous Immune System After Intradermal Antigen Injections

Study Overview
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Brief Summary
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The goal of the vaccination is to induce humoral (antibody) and intracellular (T lymphocyte) responses. Various data show that intradermal vaccination is more efficient than intramuscular vaccination: the humoral response is statistically better after intradermic vaccination, compared to intramuscular vaccination, even in target populations such as older subjects or immunosuppressed patients

Detailed Description
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Realisation of 2 groups of healthy subjects with 2 intradermal injection of 50 µl of tuberculin + 2 intradermal injection of 50 µl of physiological serum. 5 biopsy performed on 2 Tuberculin injection site, 2 physiological serum site and 1 on healthy skin site : Group A : healthy young subjects(18 to 40 years old) : 18 subjects Sub-group A1 (biopsy 30 minutes after injection) : 6 subjects Sub-group A2 (biopsy 4 hours after injection) : 6 subjects Sub-group A3 (biopsy 72 hours after injection) : 6 subjects Group B : healthy old subjects(60 to 75 years old) : 18 subjects Sub-group B1 (biopsy 30 minutes after injection) : 6 subjects Sub-group B2 (biopsy 4 hours after injection) : 6 subjects Sub-group B3 (biopsy 72 hours after injection) : 6 subjects Determination of the best time (30 minutes, 4 hours or 72 hours)for intradermal vaccination caracteristics after group A and B data analysis. From these results, last group of immunosuppressed patients performed:Group C with 6 immunosuppressed patients (18 to 60 years old)

Official Title
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LBP003: Characteristics of the Changes in the Cutaneous Immune System After Intradermal Antigen Injections

Conditions
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Healthy, Immunosuppressed

Intervention / Treatment
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* Biological: Tuberculin antigen


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Both genders eligible for study. Female participants must use a contraceptive method. Tuberculin skin test between 1 and 15mm Participants must be able to understand and sign the Informed Consent, and comply with all aspects of the protocol. Subjects registered in a social security system or with health insurance cover First group: Healthy subjects aged from 18 to 75 years with 2 subgroups: 18-40 and 60-75 year old subjects Second group: Patients with kidney transplants of longer than 6 months on immunosuppressive treatment Exclusion Criteria: Pregnant or lactating women. Previous allergic reaction to tuberculin skin test Active skin disease on testing zone Patients with a clinically significant disease (chronic, recurrent or active) Local or systemic medication which interacts with the outcome measures. Patients deprived of their civic rights, in custody, or under the care of a tutorial, judiciary or administrative body. Patients relevant of a protection measure Patients in a critical medical situation Patients with a personal situation evaluated by the investigator as unable to give an optimal participation in the study, or where it could constitute a risk for the patient Linguistic barrier or psychological profile disabling the patient from signing the consent form Patient still in an exclusion period following participation in another clinical trial Patients having earned more than 4500€ in indemnities following participation in clinical trials during the last 12 months, including this study For group 1 only: Systemic corticotherapy or immunosuppressive treatment within the 2 weeks before first visit or within 2 weeks before the third visit or the biopsies. Systemic non anti-inflammatory drugs the week before the first visit or within 1 week before the third visit or the biopsies

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Intervention/Treatment |
| --- |
|Biological: Tuberculin antigen|2 intradermal injection of 50µl of antigen + 2 intradermal injection of 50µl of physiological serum|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Cellular changes of recruited cells by immunochemical and histological analysis in the three groups of subjects (healthy young subjects, healthy old subjects and immunosuppressed patients) |  | 30 minutes, 4 hours or 72 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Molecular analysis of cytokines and chemokines involved in recruitment and/or function of immunocompetent cells |  | 30 minutes, 4 hours or 72 hours | 
| Comparison of changes in the cutaneous immune system between the three groups of subjects |  | 30 minutes, 4 hours or 72 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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innate immunity, Cutaneous immune system, tuberculin skin tests, intradermal vaccination, pathophysiology, Healthy subjects, Immunosuppressed patients