Prevalence of Bacteria Following Endodontic Treatment

Study Overview
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Brief Summary
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Sampling of accessed root canals with endodontic paper points during treatment and immediately pre-obturation of endodontic root canals was carried out.

Detailed Description
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Following ethical approval (REC ref: 05/Q0705/051), informed consent was obtained from patients undergoing root canal treatment.The teeth included in the study had been referred to a specialist centre for the treatment of endodontic infection. They all responded negatively to vitality tests and presented pre-operative apical radiolucencies. The source population were referred by their general dental practitioner into specialist endodontic clinics of Guy's Hospital, King's College London Dental Institute.Treatments on the teeth were carried out by a total of 19 postgraduate endodontic students.

Official Title
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Prevalence and Phenotype of Oral Enterococcus Faecalis

Conditions
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Root Canal Infection

Intervention / Treatment
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* Diagnostic Test: Detection of residual vital bacteria in the root canal space


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients with single or multi-rooted teeth undergoing root canal treatment. Exclusion Criteria: For certain aspects of the study secondary treatments and vital teeth are excluded.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Primary treatments<br>Patients undergoing primary root canal treatments. Only teeth which have not previously been accessed are included in this cohort.The source population are adults aged 18-65, referred by their general dental practitioner into specialist endodontic clinics of Guy's Hospital | Diagnostic Test: Detection of residual vital bacteria in the root canal space<br>* Detection of residual vital bacteria in the root canal space. No intervention, as it is an observational study. Vital bacteria are looked for in the samples taken from the root canal space.<br>* Other names: Detection of root canal infection;|
| Re-treatments<br>Patients undergoing re-treatments. Only teeth which had a previous root canal treatment which failed are included in this cohort.The source population are adults aged 18-65, referred by their general dental practitioner into specialist endodontic clinics of Guy's Hospital | Diagnostic Test: Detection of residual vital bacteria in the root canal space<br>* Detection of residual vital bacteria in the root canal space. No intervention, as it is an observational study. Vital bacteria are looked for in the samples taken from the root canal space.<br>* Other names: Detection of root canal infection;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Detection of residual bacteria during and post root canal treatment | Using a vital fluorescent stain bacteria sampled from the root canal space were detected during and post root canal treatment. | June 2016 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Bacteria, Root canal treatment, Endodontics