Mushroom Blend on Oxygen Kinetics

Study Overview
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Brief Summary
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Purpose: To determine the acute effects of a mushroom blend vs. placebo on oxygen kinetics, aerobic power and time to fatigue in healthy adults.

Detailed Description
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Participants: Twenty-eight, healthy, active, recreationally-trained adults ages 18-35. Procedures (methods): All subjects will report to the Applied Physiology Laboratory in Fetzer Rm 025 for phase I of the study for a total of 7 visits. A randomly selected subset of 8 participants will continue to phase II, and return to the lab for an additional 3 visits to evaluate feasibility. Visit 1 will consist of a physical, enrollment and consenting. Visits, 2, 3, and 4 will be separated by a minimum of 24 hours. During visit 2, participants will perform a maximal oxygen consumption (VO2max) test on a cycle ergometer. During visit 3 participants will be asked to complete a 6-minute oxygen kinetics test on a cycle ergometer; lactate threshold and oxygen saturation will be measured at baseline, minutes 2, 3 and 6 during the 6 min ride. During visit 4, participants will perform a 3-minute critical power (CP) test and will provide a saliva sample, to be measured for cortisol levels, immediately prior to and after the CP test. Following visit 4, participants will be randomly assigned, in a double-blind fashion, to either a placebo or mushroom blend treatment group. Participants in the placebo and treatment groups will be asked to consume 2 servings, 3 times daily for 7 days. Following supplementation, participants will return to the laboratory for visits 5-7, each of which will be separated by a minimum of 24 hours. The same protocol performed during visits 2-4 will be repeated in the same order for visits 5-7; the VO 2max test, the 6 min oxygen kinetics test, and the CP test with a pre and post saliva sample. The randomly selected subset of 8 participants will continue into phase II (n=4 for treatment; n=4 for placebo). During phase II, the 8 participants in the placebo and treatment groups will be asked to consume 2 servings, 3 times daily for 14 additional days to determine feasibility of a longer supplementation phase. Following supplementation, the same protocol performed during visits 2-4 will be repeated in the same order for visits 8-10, each of which will be separated by a minimum of 24 hours.

Official Title
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Acute Effects of a Mushroom Blend on Oxygen Kinetics, Aerobic Power and Time to Fatigue

Conditions
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Oxygen Utilization, Oxygen Kinetics

Intervention / Treatment
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* Dietary Supplement: Mushroom Blend
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Participant has been involved in exercise program for a minimum of one year prior to recruitment (≥ 3 d/wk) Participant is able to exercise (i.e. no existing musculoskeletal injury) Participant is willing and able to comply with the protocol Participant is apparently healthy and free from disease, as determined by a health history questionnaire and physical Participant agrees to abstain from smoking, caffeine, tobacco, and alcohol before testing days Participant agrees to abstain from exercise 24 hours prior to each testing visit Participant is not allergic to mushrooms Exclusion Criteria: Participant has a BMI ≤ 28 kg/m² (pre-screening inclusion) Having a history of medical or surgical events that may significantly affect the study outcome, including cardiovascular disease, metabolic, renal, hepatic, or musculoskeletal disorders Participant is using, or has used one of the following dietary supplements within 12 weeks prior to enrollment: Beta-alanine, Creatine, Carnosine or Taurine Participant has lost or gained greater than ten pounds within the previous 3 month Participant is currently enrolled in another clinical trial. Participant has a known allergy or sensitivity to any ingredient in the test product or placebo (determined from health history questionnaire)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Mushroom Blend<br>4 grams daily; oral; 7 and 21 days | Dietary Supplement: Mushroom Blend<br>* Scivation<br>* Other names: Cordyceps;|
| Placebo Comparator: Placebo<br>4 grams maltodextrin, oral, 7 and 21 days | Dietary Supplement: Placebo<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oxygen kinetics | oxygen uptake and utilization using indirect calorimetry during a 6 min steady-state exercise bout | during a 6 min | 
| Critical Power | 3 min all-out cycling test to measure anaerobic and aerobic performance | during a 3 min | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Lactate |  | baseline, 2min, 3min, 6min (pre-post supplementation) | 
| Cortisol |  | during a 6 min | 
| Maximal Oxygen Consumption | oxygen consumption and utilization using indirect calorimetry during a graded exercise test | during a graded exercise test to exhaustion | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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cordyceps, aerobic capacity, exercise performance, fatigue