Dental Stem Cells and Bone Tissue Engineering (CELSORDINO)

Study Overview
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Brief Summary
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The main limitation in bone regeneration is the lack of vascularization of the newly shaped tissue. The main objective of this project is to check if the simultaneous differentiation of dental mesenchymal stem cells toward osteoblastic and endothelial lineage permits to obtain a new pre-vascularized tissue engineered bone construct. We will then evaluate the boosting effect of the conditionned medium on cell differentiation and production of a prevascularized bone construct.

Detailed Description
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This project aims to develop a new pre-vascularized tissue engineered bone construct, using human cells of a simple and non invasive tissue source: dental pulp. We will isolate mesenchymal stem cells from dental tissue of wisdom teeth, extracted from patients aged between 13 and 17 years old. The objectives are: (i) to assess in combination the endothelial differentiation and osteoblastic differentiation of dental pulp stem cells (immature third molar); (ii) to study the boosting effect of the conditionned medium on cell differentiation and production of a prevascularized bone construct. The differentiation will be monitored by evaluation of biosynthesized matrix, bone mineralization (immunohistochimie, Westen Blot), histological response (Von Kossa, Alizarin Red) and presence and quantification of pseudo-vascular structures (angiogenesis assay), compared to reference value (commercial differentiation medium).

Official Title
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Dental Stem Cells and Bone Tissue Engineering

Conditions
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Tooth, Impacted

Intervention / Treatment
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* Other: non-interventional study


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: patients aged between 13 and 17 years, who will come to Dental Service of University Regional Hospital Center of Nancy in order to have a tooth avulsion. patient who has received a complete information about research organization and who is not opposed to the use of his own data. patient who the representatives of parental authority have received a complete information about research organization and who are not opposed to the use of children's data. Exclusion Criteria: patients with local or general pathology

Ages Eligible for Study
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Minimum Age: 13 Years
Maximum Age: 17 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: non-interventional study|teeth collection|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Bone mineralization | Measured parameter is equal or superior to reference value (commercial differentiation medium) | Day 28 of cell culture | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| presence and quantification of pseudo-vascular structures | Measured parameter is equal or superior to reference value (commercial differentiation medium) | Day 28 of cell culture | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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bone tissue engineering, dental stem cell differentiation