Clinical Study to Evaluate the Safety and Feasibility of spCART-269 Injection in the Treatment of MM

Study Overview
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Brief Summary
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The trial is a single arm, single-center, non-randomized phase I clinical trial which is designed to evaluate the safety and efficacy of spCART-269 in treatment of relapsed or refractory multiple myeloma patients.

Detailed Description
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This study plans to enroll 10 patients to assess the safety and efficacy of spCART-269. Subjects who meet the eligibility criteria will receive a single dose of spCART-269 injection. The study will include the following sequential phases: Screening, Pre-treatment (Cell product preparation; Lymphodepleting Chemotherapy), Treatment and follow-up.

Official Title
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Clinical Study to Evaluate the Safety and Feasibility of Targeting CD269 Chimeric Antigen Receptor Engineered T Cell (spCART-269) Injection in the Treatment of CD269-positive Multiple Myeloma

Conditions
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Multiple Myeloma

Intervention / Treatment
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* Biological: Targeting CD269 chimeric antigen receptor engineered T cells


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The patient was diagnosed as active MM according to the diagnostic criteria of the International Myeloma Working Group (IMWG) The patient meets any of the following: Have received at least 3 treatment options in the past and include alkylating agents, proteasome inhibitors and immunomodulators; If the patient has received a regimen containing proteasome inhibitor and immunomodulator for at least 2 courses, and the effect is not good (such as disease progression within 60 days of treatment) Voluntary participation in clinical research and signing informed consent Age 18-65, regardless of gender Expected survival time is greater than 12 weeks If the patient has received autologous hematopoietic stem cell transplantation in the past, a 90-day interval is required Normal bone marrow hematopoietic function, blood routine: hemoglobin ≥ 100 g/L; absolute neutrophil ≥ 1.5×10^9/L; platelet count ≥ 100×10^9/L Liver function: serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤ 2.5 (ULN) times the upper limit of normal value (if abnormal liver function is mainly caused by tumor infiltration, it can be ≤ 5 times the upper limit of normal value (ULN) )), bilirubin <2.0 mg/dL Renal function: BUN is 9-20 mg/dL, serum creatinine ≤ 1.5 times the upper limit of normal (ULN), endogenous creatinine clearance rate ≥50 ml/min Serum virus EBV, CMV, HBV, HCV, HIV and syphilis antibodies are negative Heart function: good hemodynamic stability, left ventricular ejection fraction (LVEF) ≥ 45% ECOG physical status score 0-2 Possess apheresis or sufficient venous access for venous blood, and no other contraindications for leukocyte separation T cells can be successfully expanded in vitro Women of childbearing age who provide negative reports of pregnancy tests with serum or urine before reinfusion Adults with fertility requirements, regardless of sex, contraception within one year after treatment Exclusion Criteria: ECOG score ≥ 3 points Female patients during pregnancy or lactation Pathological examination revealed malignant tumor cells with T cell origin Organ failure: Heart failure grade Ⅲ and Ⅳ; liver reaches Child-Turcotte liver function grade C; renal failure and uremia; respiratory failure; consciousness disorder Patients with acute or chronic GVHD after allogeneic hematopoietic transplantation, or using hormones or immunosuppressants within 30 days Patients with HIV infection or active hepatitis There are other uncontrolled active infections Those who may be allergic to cytokines Those who have used any gene therapy products Those who participated in other clinical studies 4 weeks before enrollment (except those who did not receive treatment in clinical studies) Patients with systemic autoimmune diseases or immunodeficiency diseases Definite neuropathy or psychosis, including authors of dementia or epilepsy Those with lung or intestinal tumor infiltration Patients that other researchers think are not suitable for enrollment

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: spCART-269<br>spCART-269 administered by intravenous (IV) infusion | Biological: Targeting CD269 chimeric antigen receptor engineered T cells<br>* Targeting CD269 chimeric antigen receptor engineered T cells, single infusion intravenously<br>* Other names: spCART-269;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Occurrence of study related adverse events | Incidence and severity of Treatment emergent adverse events | 12 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Overall response rate (ORR) |  | 12 months | 
| Duration of remission (DOR) |  | 12 months | 
| Progression free survival (PFS) |  | 12 months | 
| Overall survival (OS) |  | 12 weeks, 6 months, 12 months |