Myocardial Deformation Before and After Birth

Study Overview
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Brief Summary
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Knowledge on the changes in myocardial function in the last weeks before birth and during the first year of life is limited. Through fetal and post-natal echocardiography we intend to describe these changes using myocardial tissue recognition techniques (Speckle tracking echocardiography and Tissue Doppler echocardiography) in healthy neonates, born to term of healthy women after uncomplicated pregnancies. We will compare the findings in this cohort to a cohort of neonates born to term of women with severe pre-pregnancy obesity. Sick neonates in intensive care units with various cardiac and non-cardiac conditions are often exposed to treatment that may affect both their cardiac function and important echo-variables per se. Using echocardiography, we will examine these changes in neonates treated with blood transfusion, catecholamines and in those treated with Ibuprofen due to a haemodynamic significant arterial duct.

Detailed Description
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Before, during and after birth major changes take place in the cardiovascular system. The last weeks prior to birth there is an oxidative stress and after birth, there is normally a large step up in blood oxygen tension when the lungs take over oxidation of the blood. Simultaneously there are changes in the vascular resistance in both the pulmonary- and systemic part of the circulation and ductus venous, the arterial duct, and foramen ovale close. Combined these will significantly alter the physical conditions under which the myocardium is supposed to work. This project will have five arms, all based on measurements of myocardial function and coronary perfusion by speckle tracking echocardiography (STE) in the last trimester of the pregnancy and in newborns with different gestational age and in sick newborns where myocardial function is affected by medical treatment or is an important part of the clinical situation. Obtaining normal values for strain during the last trimester of the pregnancy and the first days after birth in child born to term. Hypothesis: After birth, there will be higher STE values in the left ventricle than before birth. This will increase further the first days post-partum in parallel with an altered hemodynamic situation and oxygen demand of the infant. The STE changes will take place at the same time, as there is an increased Doppler velocity in the aorta, the pulmonary artery and the coronary arteries Mothers (N= 150) will be recruited when they are coming to a routine fetal ultrasound scan. It will be made an echo-examination once per week from week 37 until birth. After birth the degree of placental transfusion (late cord clamping or not) will be noticed. Ventricular size, Doppler velocities in the ascending aorta and pulmonary artery, STE and coronary flow during the first three days of life and one year of age will be assessed Evaluate the effect of maternal obesity on cardiac function in newborns up to one year of age. Hypothesis: Maternal sever obesity will result in reduced cardiac function in their neonates. However, cardiac function will normalize within the first year of life. A reduction in afterload will reduce strain measured by STE and coronary. Mothers (N=75) with sever obesity will be recruited from the outpatient clinic for obese women. First day after birth, the newborn will have a comprehensive echocardiographic examination within the first few hours, then the two following days, at the day equivalent to gestational age 42+0 weeks and finally at 1 year of age. Cardiac morphology, ventricular sizes and Doppler velocities in the ascending aorta and pulmonary artery as well as a strain measured by STE for both ventricles will be assessed. Fetal malformations or diseases will result in exclusion from the study. Measuring deformation when preload is increased by intravenous fluid treatment and blood transfusion Hypothesis: Increased preload in the form of saline infusion or blood transfusion will result in a temporary increase of STE and coronary blood flow. Newborns (N=25 blood transfusion and 25 NaCl-infusion) who need infusion of sodium chloride (NaCl 9mg) / ml or transfusion of packed red blood cellssuspended in 100 mL of saline- adenine-glucose-mannitol (SAGMAN) will be recruited. To obtain rehydration or optimization of intravascular volume it is common to provide NaCl 9 mg/ml, 10-20 ml/kg in 10-120 minutes. Blood transfusion is used in severe anemia and hemolytic diseases. The regular blood transfusion dose is SAGMAN packed red blood cells10-15ml/ kg body weight over 2-3 hours. Children requiring exchange transfusion, with heart failure or congenital heart defects will be excluded. Measuring deformation when afterload and heart rate are increased during infusion of catecholamines. Hypothesis: Dopamine, epinephrine and/ or nor-epinephrine will increase heart rate and blood pressure and due to this increase the strain measured by STE, coronary blood flow, and Doppler velocity in the aorta pulmonary artery. Newborns (N=25) who need dopamine (2.5 to 15 ug/kg /min iv.) and or epinephrine / nor-epinephrine to maintain an adequate blood pressure will be recruited. Only children where an echo-exam before starting treatment is possible will be recruited. If the treatment does not achieve a rise in the blood pressure above the 2.5 percentile for gestational age, the baby will be excluded. Measuring deformation when afterload (volume) is reduced following pharmacological closure of a patent ductus arteriosus. Hypothesis: A reduction in afterload will reduce strain measured by STE and coronary. Patients (N=25) with a suspected patent ductus arteriosus (PDA) are always referred to echocardiography before starting treatment with Indomethacin and after the treatment has stopped. Present clinical procedures for closure of the ductus will be followed, . Time of echocardiography will be standardized to just before and at least 12 hours after the last dose of Ibuprofen has been provided (t½ ≈ 2.5-11 hrs.).

Official Title
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Myocardial Deformation Before and After Birth, and Under Altered Preload, Afterload and Heart Rate in New-borns

Conditions
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Myocardial Dysfunction, Newborn Morbidity, Maternal Obesity

Intervention / Treatment
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* Drug: Blood transfusion, Ibuprofen, or epinephrine, norepinephrine or dopamine


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Normal pregnancies Newborns in need of blood transfusion Newborns in need of medical closure of the arterial duct Maternal obesity Exclusion Criteria: Abnormal pregnancies except maternal obesity Congenital heart defects Other malformation syndromes

Ages Eligible for Study
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Maximum Age: 3 Weeks

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Fetuses of normal weight women<br>Fetuses of normal pregnancies of normal weight mothers are included from gestational week 37 | |
| Fetuses of severely obese women<br>Fetuses of normal pregnancies of severly obese mothers are included from gestational week 37 | |
| New-borns in need of blood transfusion<br>Neonates mainly receive blood due to blood loss, often because of repeated blood sampling | Drug: Blood transfusion, Ibuprofen, or epinephrine, norepinephrine or dopamine<br>* Treatment/ intervention given according to standard protocols at the Neonatal Intensive Care Unit<br>* Other names: Blood pressure elevation;|
| New-borns in need for closure of the arterial duct<br>Neonates mainly receive medication (Ibuprofen) because of symptoms like apnea, due to their patent arterial duct. | Drug: Blood transfusion, Ibuprofen, or epinephrine, norepinephrine or dopamine<br>* Treatment/ intervention given according to standard protocols at the Neonatal Intensive Care Unit<br>* Other names: Blood pressure elevation;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in myocardial function | Per cent change in longitudinal global strain | Maximum 1 year and 5 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Tissue doppler, Spackle tracking echocardiography, Neonates, Infants, Maternal obesity