Factors of Steroid Dependency in Idiopathic Nephrotic Syndrome

Study Overview
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Brief Summary
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The primary purpose of the study is to identify the factors of steroid dependency in childhood idiopathic nephrotic syndrome. The steroid dependency is defined by a relapse of nephrotic syndrome within the 3 weeks that follow the withdrawal of steroid therapy after the first manifestation. Different clinical and biological factors will be analyzed: age of first manifestation, delay of remission, ethnicity, and preceding viral infection, geolocalization in the Parisian area, genoprevalence of herpes viruses and polymorphisms in the genes involved in the response to steroid therapy.

Detailed Description
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Patients with a steroid dependent nephrotic syndrome will be compared to those with a non steroid dependent form of nephrotic syndrome at the end of the first manifestation. An additional blood volume will be sampled in patients and controls during a scheduled biological check-up for the initial disease. Genetics factors will be checked using a DNA chips devoted to the response to steroid therapy and viral genome of EBV, CMV, HHV7 will be searched for using PCR reaction in total blood DNA extract.

Official Title
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Factors of Steroid Dependency in Idiopathic Nephrotic Syndrome

Conditions
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Idiopathic Nephrotic Syndrome

Intervention / Treatment
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* Genetic: DNA chip dedicated to the pharmacogenetic of steroids


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: children below 16 years first manifestation of nephrotic syndrome steroid sensitivity according to the recommendations of the french pediatric society nephrology Exclusion Criteria: patients who don't stay in Paris during the study

Ages Eligible for Study
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Minimum Age: 6 Months
Maximum Age: 16 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| children with idiopathic nephrotic syndrome<br>children below 16 years of age with a steroid dependent nephrotic syndrome | Genetic: DNA chip dedicated to the pharmacogenetic of steroids<br>* direct DNA extraction from peripheral blood cells<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| define pharmacogenetic factors of steroid dependency |  | 28.5 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| parameters of the immune response against herpesvirus, specially the cellular production of interferon induced by specific viral peptides |  | 28.5 months | 
| Dynamic of the epidemiology of idiopathic nephrotic syndrome in the Paris Area | Number of participants with idiopathic nephrotic syndrome in Paris Area to determinate if clusters cases exist. | 28.5 months | 
| Analysis of cortico-dependence factors | Comparison of polymorphisms of cortico-dependent patients with non- cortico-dependent patients | Day 1 | 
| Analysis of susceptibility factors that generate the appearance of the Idiopathic Nephrotic Syndrome | Comparison of polymorphisms of all patients suffering from the Idiopathic Nephrotic Syndrome, with general population | Day 1 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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interferon, Proteinuria, idiopathic, nephrotic syndrome, herpesvirus, epidemiology, immune response, steroid dependency, nephrotic disease: Idiopathic nephrotic syndrome