Influence of Tart Cherry Juice Intervention on Vascular Function

Study Overview
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Brief Summary
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Diets containing fruit rich in anthocyanins have been shown (in meta-analyses) to be beneficial for health, but intervention trials have shown inconsistent results. In this preliminary study we want to investigate the influence of a tart-cherry juice intervention on vascular function and explore metabolite profiles that may offer insight into their mechanism of action

Detailed Description
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Cardiovascular disease (CVD) is the primary cause of morbidity and mortality. Tart cherries (TC) can positively impact major risk factors of CVD, activities thought to be related to their phytochemical content. However, data from human trials is less clear. TC have been shown to reduce systolic blood pressure (SBP) after acute, short-term and chronic intake, and to improve vascular dysfunction, presumably due to their antioxidant and anti-inflammatory activities. Other studies have not shown benefits of TC on SBP or vascular function and studies are difficult to interpret with different populations, dosing and durations being used as well as a lack of understanding for any underlying mechanisms. To account for the latter, the use of metabolomics has become increasingly popular in nutritional research. Therefore, the aim of this preliminary study was to characterise the effects of TC supplementation on vascular function with a 4-week intervention and explore if metabolomics can provide mechanistic evidence and this will inform for future chronic studies with TC juice.

Official Title
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Influence of Tart Cherry Juice Intervention on Vascular Function and the Urinary Metabolite; a Preliminary Study to Inform Study Design

Conditions
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Dietary Exposure, Metabolic Disturbance

Intervention / Treatment
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* Dietary Supplement: Cherry juice
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: aged 19-40 no diagnosed issues with the ability to consume fruit juice no known food intolerances to fruits Exclusion Criteria: participants that are cigarette smokers participants diagnosed with hypertension participants diagnosed with any form of cardiometabolic diseases participants that require any prescribed prescriptions excluding the contraceptive pill participants that are regular consumers of cherries (>100 g/d) participants that consume any antioxidant supplements

Ages Eligible for Study
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Minimum Age: 19 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: Randomised controlled trial
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Placebo<br>Participants supplied with 30 ml of a low fruit (<1%) cordial mixed with maltodextrin and protein diluted in 100 ml water twice daily, once in the morning and evening. | Dietary Supplement: Placebo<br>* A tart cherry juice placebo<br>|
| Experimental: Cherry juice<br>Participants supplied with 30 ml of a tart cherry juice concentrate (CherryActive, United Kingdom; (containing 36.8 mg of anthocyanins) diluted in 100 ml of water twice daily, once in the morning and evening. | Dietary Supplement: Cherry juice<br>* A tart cherry juice intervention<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Systolic and diastolic blood pressure | Measurement of systolic and diastolic blood pressure before and after the intervention | Change in blood pressure following 4 weeks intervention | 
| Arterial pulse wave velocity | Measurement of pulse wave velocity before and after the intervention | Change in pulse wave velocity following 4 weeks intervention | 
| Digital volume pulse | Measurement of digital volume pulse before and after the intervention | Change in digital volume pulse following 4 weeks intervention | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolomics | Ion intensities of ca 5000 metabolites in urine samples before and after the intervention | Changes to urinary metabolite profiles following 4 weeks intervention | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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cherry, anthocyanin, vascular function, metabolomics