Water Intake and Glycemic Response : Preliminary Study

Study Overview
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Brief Summary
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Glycemic index concept is widely used to determine impact of a food to blood glucose. Several studies showed that water content and intake after meal may influence glycemic response of the meal and thus have an effect on the result of GI measurement. Currently these studies found conflicting results. Therefore, this study aims to determine whether water intake, specifically after meal, may influence the glycemic response and glycemic index value of the meal.

Detailed Description
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Glycemic index methods are performed according to Food and Agricultural Organization/WHO recommendations.

Official Title
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Water Intake and Glycemic Response : Preliminary Study

Conditions
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Healthy

Intervention / Treatment
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* Other: Glucose Ref - Std
* Other: Glucose Ref and 450 ml water
* Other: White Bread with 250 ml water
* Other: White Bread and 700 ml water
* Other: White Bread with 125 ml water (twice)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age between 20-40 years fasting blood glucose 70-99 mg/dL Indonesians Exclusion Criteria: having diabetes smoking if women, pregnant or lactating being allergic to food used in this study having gastrointestinal disturbance in regular medication refusal to sign the consent form

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Glucose Ref - Std<br>250 ml glucose solution with 50 gram available carbohydrate per serving, used as reference | Other: Glucose Ref - Std<br>* First, fasting blood glucose was measured. Then, subjects consumed the glucose sample in 5-10 minutes and blood sugar level were measured at 0', 15', 30', 45', 60', 90', and 120'<br>|
| Experimental: Glucose Ref and 450 ml water<br>250 ml glucose solution with 50 gram available carbohydrate per serving. Sample was consumed with additional water intake @150 ml at 45, 75, and 105 minutes after meal consumption | Other: Glucose Ref and 450 ml water<br>* First, fasting blood glucose was measured. Then, subjects consumed the glucose sample in 5-10 minutes. Subjects given additional 150 ml water at 45, 75, and 105 minutes and blood sugar level were measured at 0', 15', 30', 45', 60', 90', and 120<br>|
| Experimental: White Bread with 250 ml water<br>White bread with 50 gram available carbohydrate was consumed with 250 ml water immediately after meal consumption | Other: White Bread with 250 ml water<br>* First, fasting blood glucose was measured. Then, subjects consumed the samples in 10-15 minutes and blood sugar level were measured at 0', 15', 30', 45', 60', 90', and 120'<br>|
| Experimental: White Bread and 700 ml water<br>White bread with 50 gram available carbohydrate was consumed with 250 ml water immediately after meal consumption. Moreover, subjects given additional water intake @150 ml at 45, 75, and 105 minutes after meal consumption | Other: White Bread and 700 ml water<br>* Sample consumed with 250 ml water immediately after meal consumption, and additional 150 ml water at 45, 75, and 105 minutes. First, fasting blood glucose was measured. Then, subjects consumed the samples in 10-15 minutes and blood sugar level were measured at 0', 15', 30', 45', 60', 90', and 120'<br>|
| Experimental: White Bread with 125 ml water (twice)<br>Sample consumed with 125 ml water immediately after meal consumption, and additional 125 ml water after 60 minutes | Other: White Bread with 125 ml water (twice)<br>* Sample consumed with 125 ml water immediately after meal consumption, and additional 125 ml water after 60 minutes First, fasting blood glucose was measured. Then, subjects consumed the samples in 10-15 minutes and blood sugar level were measured at 0', 15', 30', 45', 60', 90', and 120'<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 2 h postprandial blood glucose level (Glycemic Response) |  | fasting 0 min, 15 min, 30 min, 45 min, 60 min, 90 min, 120 min | 
| Glycemic index value of meal (white bread) | The glycemic index is a calculated from measurements of area under the glucose curve after the food is eaten, divided by the corresponding area after the control food is eaten. The value is multiplied by 100 to represent a percentage of the reference food. | fasting 0 min, 15 min, 30 min, 45 min, 60 min, 90 min, 120 min | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Glycemic Index, Water Intake, Glycemic Response