Effects of Roux-en-Y Gastric Bypass on Gastro-intestinal Hormone Secretion and Brain Responses Food Pictures

Study Overview
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Brief Summary
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This is a cross-sectional study enrolling 11 weight-stable, formerly obese female patients 1-5 years after Roux-en-Y gastric Bypass (RYGB) 11 age- and weight-matched female subjects 10 normal weight (Body Mass Index 19-25 kg/m2) subjects The following measurements will be performed in each participants in basal conditions (after an overnight fast) and after ingestion of a standard meal electroencephalographic recordings of visually evoked potential after presentation of food- or non-food pictures plasma concentration of metabolites (glucose, fatty acids), glucoregulatory hormones (insulin, glucagon), gastro-intestinal hormones (Gastric Inhibitory Polypeptide (GIP), Glucagon-like Peptide-1 (GLP1), ghrelin,Cholecystokinin (CCK), and bile acids Aims of this observational study are to evaluate the effects of RYGB on brain perception of food related visual stimulation to search for relationships between RYGB effects on brain responses and gastro-intestinal hormone secretion

Official Title
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Dynamic Effect of Gastrointestinal Hormones on Cerebral Mechanisms Involved in Brain Perception of Food Pictures After Bariatric Surgery

Conditions
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Obesity

Intervention / Treatment
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* Other: standard test meal


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: good apparent health gender: female age 18-50 non-smoker Exclusion Criteria: diabetes or antidiabetic medication consumption of more than 50g alcohol per week vegetarian or any special diet

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| post Roux-en-Y-gastric bypass<br>Formerly obese females 1-5 years after gastric bypass | Other: standard test meal<br> <br> |
| matched controls<br>non-operated females age-and weight-matched to post Roux-en-Y-gastric bypass subjects | Other: standard test meal<br> <br> |
| healthy young controls<br>non overweight (BMI between 19 and 25 kg/m2) healthy females | Other: standard test meal<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes of visually-evoked EEG potentials after ingestion of a meal | Recording and analysis of visual evoked potentials (VEPs) while patients categorize photographs of foods and non-food kitchen utensils | basal, 120 min postprandial and 240 postprandial | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| changes in plasma triglyceride concentration after ingestion of a meal | Measurement of triglyceride concentrations at 30-60 min intervals in basal conditions (after an overnight fast) and over 6 hours after ingestion of a standard meal) | basal, 90 min postprandial and 180 postprandial | 
| Changes in plasma glucoincretins (GLP1 and GIP) concentrations after ingestion of a meal | Measurement of GLP1 and GIP concentrations at 30-60 min intervals in basal conditions (after an overnight fast) and over 6 hours after ingestion of a standard meal) | basal, 90 min postprandial and 180 postprandial | 
| Changes in plasma cholecystokinin concentrations after ingestion of a meal | Measurement of cholecystokinin concentrations at 30-60 min intervals in basal conditions (after an overnight fast) and over 6 hours after ingestion of a standard meal) | basal, 90 min postprandial and 180 postprandial | 
| Changes in plasma bile acids concentrations after ingestion of a meal | Measurement of bile acids concentrations at 30-60 min intervals in basal conditions (after an overnight fast) and over 6 hours after ingestion of a standard meal) | basal, 90 min postprandial and 180 postprandial | 
| changes in plasma glucoregulatory status after ingestion of a meal | Measurement of glucose, insulin, glucagon and non-esterified fatty acid concentrations at 30-60 min intervals in basal conditions (after an overnight fast) and over 6 hours after ingestion of a standard meal) | basal, 90 min postprandial and 180 postprandial | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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roux-en-Y-gastric bypass, brain responses to food, gastrointestinal hormones, bile acids