Effects of Protein Blends on Muscle Protein Synthesis in Healthy Older Adults

Study Overview
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Brief Summary
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During aging there is a tendency for muscle protein synthesis (growth) to become less efficient and the resulting consequence leads to reduced muscle mass (sarcopenia) which can affect strength and mobility. Protein consumption may be one opportunity to alleviate this problem especially when consumed in appropriate amounts following resistance training. This study is designed to determine if a soy dairy protein blend, shown to be effective in younger adults, will produce beneficial muscle protein synthesis in older healthy adults following resistance exercise.

Official Title
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A Randomized, Controlled Double Blind Acute Study: Effects of Protein Blends on Muscle Protein Synthesis and Breakdown in Healthy Older Adults

Conditions
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Muscle Synthesis

Intervention / Treatment
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* Dietary Supplement: Soy Dairy Protein Blend
* Dietary Supplement: Positive Control (Dairy Whey Protein)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 55-70 yr olds Stable Body weight Exclusion Criteria: Exercise training (>2 weekly sessions of moderate to high intensity aerobic or resistance exercise) Significant heart, liver, kidney, blood, respiratory disease or thyroid issues Peripheral vascular disease Orthopedic injury that prohibits participation in the exercise training Diabetes mellitus or other untreated endocrine disease Active cancer (all groups) and history of cancer Acute infectious disease or history of chronic infections (e.g. TB, hepatitis, HIV, herpes) Neurologic Injury or disease Recent (within 6 months) treatment with anabolic steroids, or corticosteroids. Alcohol or drug abuse Tobacco use (smoking or chewing) Malnutrition (BMI < 20 kg/m2, hypoalbuminemia, and/or hypotransferrinemia) Obesity (BMI > 30 kg/m2) Low hemoglobin levels (below normal values) Food allergies Taking dietary supplements such as green tea, omega 3 fatty acids, etc. Females

Ages Eligible for Study
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Minimum Age: 55 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Soy Dairy Protein Blend<br>30 grams of Soy Dairy Protein Blend given as a single beverage following leg resistance exercise | Dietary Supplement: Soy Dairy Protein Blend<br> <br> |
| Active Comparator: Positive Control (Dairy Whey Protein)<br>30 grams of Dairy Whey Protein will be given as a single beverage following leg resistance exercise | Dietary Supplement: Positive Control (Dairy Whey Protein)<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Fractional Synthetic Rate (Muscle Protein Synthesis) | The fractional synthesis rate (FSR) of mixed muscle proteins will be calculated from the incorporation rate of L-[ring-13C6]Phenylalanine into the mixed muscle proteins, and the free-tissue phenylalanine enrichment. | baseline, 3 hrs, 5 hrs | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Anti-inflammatory and Anti-oxidant Markers | Proteins will be assessed that are markers of oxidative damage and inflammation (NFkB, IL-1, IL-6) and proteolysis (MuRF, MAFBx). | baseline, 3 hrs, 5 hrs | 
| Intracellular Signalling Genes and Proteins | Phosphorylation of Mammalian Target of Rapamycin (mTOR) pathway will be measured using Western blot techniques | baseline, 3 hrs, 5 hrs | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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soy, whey, muscle, older, mTOR, sarcopenia