Identification of Epigenetic Markers Common to Obesity and Alzheimer's Disease in Women

Study Overview
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Brief Summary
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The purpose of this study is to compare average methylation of epigenetic markers on genomic DNA and some genes (APP, BACE, LRP, SorL1) involved in cerebral amyloid homeostasis: Of obese young adults and healthy young adults Of obese young adults and individuals affected by Alzheimer's disease (AD) having been obese at young adult age (<50 years old) or individuals affected by Alzheimer's disease (AD) having never been obese. The secondary purpose is to determine if there is an association between the frequency of these epigenetic markers and elements associated to the metabolic syndrome (lipidic and glycemic analysis, leptinemia, inflammation markers) and clinical ones (visceral fat mass, body mass index) in young adults.

Official Title
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Identification of Epigenetic Markers Common to Obesity and Alzheimer's Disease in Women - Pilot Study

Conditions
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Obesity, Alzheimer's Disease

Intervention / Treatment
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* Procedure: Blood sample


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Group 1: 18-50 year old women, age- (10 years) and sociocultural-matched with group 2 Non obese and never been obese 18.5 < BMI < 25 Folstein MMS > 27 Informed consent Affiliation to social security plan Group 2: 18-50 year old women, age- (10 years) and sociocultural-matched with group 1 Waist size > 88cm 30 < BMI < 45 Insulin-resistant patients (Insulin resistance index, HOMA-IR > 3.8) Obesity onset during childhood (pre-puberty period) Folstein MMS > 27 Informed consent Affiliation to social security plan Group 3: >60 year old women, age- (10 years) matched with group 4 No obesity history (18.5 ≤ BMI < 25 at adult age) Sporadic forms of mild to moderate AD with demonstration of AD physiopathologic process according to new recommendations of 2011 of National Institute on Aging and Alzheimer's Association on diagnostic guidelines for Alzheimer's disease 15< Folstein MMS ≤ 26 Informed consent Affiliation to social security plan Group 4: >60 year old women, age- (10 years) matched with group 3 Obesity history (BMI > 30 at least one time at adult age) Sporadic forms of mild to moderate AD with demonstration of AD physiopathologic process according to new recommendations of 2011 of National Institute on Aging and Alzheimer's Association on diagnostic guidelines for Alzheimer's disease 15 < Folstein MMS ≤ 26 Informed consent Affiliation to social security plan Exclusion Criteria: Group 1: <18 Patient under guardianship, curatorship or judicial protection Folate supplementation Diabetic or glucose intolerant subjects Present participation to another study with neuropsychological evaluation and/or drug administration Pregnant women Groups 2,3 and 4: <18 Patient under guardianship, curatorship or judicial protection Folate supplementation Present participation to another study with neuropsychological evaluation and/or drug administration Group 2: - Pregnant women Group 4: - Obese or overweight patients (BMI>25)

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Control group<br>Control group | Procedure: Blood sample<br> <br> |
| Other: Obese group<br>Obese group | Procedure: Blood sample<br> <br> |
| Other: Non-obese AD group<br>Non-obese AD groups | Procedure: Blood sample<br> <br> |
| Other: Obese AD group<br>Obese AD group | Procedure: Blood sample<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Methylation of CpG islands in genomic DNA and in 4 target genes involved in AD (APP, SorLA/LR11, BACE1, LRP) in obese and AD subjects |  | day 0 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| body mass index |  | day 0 | 
| visceral fat mass level |  | day 0 | 
| leptinemia by ELISA |  | day 0 | 
| insulinemia by ELISA |  | day 0 | 
| glycemia |  | day 0 | 
| triglyceridemia |  | day 0 | 
| HDL cholesterol level in blood |  | day 0 | 
| Reactive C protein level in blood |  | day 0 |