Probiotic Supplementation in Sport Performance and Gut Damage

Study Overview
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Brief Summary
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This study will be performed with the aim to evaluate the effects of probiotic supplementation on sport performance and gut damage. Participants will be divided into two groups, placebo and experimental group. With the purpose to assess the effects of probiotics on the gut damage, a strenuous exercise session will be performed, and then blood samples will be taken to observe the variation of the selected intestinal damage marker. 24 hours after this strenuous session, performance tests will be performed. After the 4 weeks of supplementation, the same procedure will be followed to evaluate the effect of probiotics.

Detailed Description
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One week before the initiation of the study participants will be required to avoid consuming drugs, alcohol, cigarettes, probiotics, and any other food or liquid which could modify gut microbiota composition. For the baseline values, height, weight, sports performance test, and a blood sample of the participants will be collected (at rest). After one week, a strenuous exercise session will be performed in order to observe the variation of the intestinal damage marker. 24 hours after that session sport performance tests will be conducted. Participants will then be randomly divided into two groups, one of which will take one probiotic capsule per day containing 10 billion colony-forming units (CFU) of Lactobacillus plantarum, and the other group will take one placebo capsule per day consisting of dextrose. The supplementation period will last 4 weeks. After the supplementation period, the same process will be followed, with the same strenuous exercise session and immediate blood sampling to determine the effects of probiotics on the intestinal damage caused by exercise. 24 hours later, performance tests will be conducted to assess the effects of probiotics on sport performance.

Official Title
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Effects of Probiotic Supplementation on Sport Performance and Gut Damage in Basketball Players

Conditions
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Sport Performance, Gut Damage

Intervention / Treatment
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* Dietary Supplement: Probiotic
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy No injuries 3 years of basketball experience Exclusion Criteria: Drug, alcohol, cigarettes, probiotic intake Injuries

Ages Eligible for Study
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Minimum Age: 16 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Experimental group<br>Probiotic intake containing 10 billions of Colony Forming Units of Lactobacillus plantarum (1 capsule per day) | Dietary Supplement: Probiotic<br>* Probiotic capsules will be composed of 10 billion CFU of Lactobacillus plantarum.<br>|
| Placebo Comparator: Placebo group<br>Placebo intake. Placebo capsules will contain dextrose (1 capsule per day) | Dietary Supplement: Placebo<br>* Capsules containing dextrose (1 capsule per day)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Intestinal-fatty acid-binding protein | Gut damage marker | 10-30 minutes after strenuous exercise blood samples will be extracted | 
| Counter movement jump test | Jumping test | 24 hours after strenuous training session. First test to be performed | 
| Running Based Anaerobic Sprint Test | Running test | 24 hours after strenuous training session. Third test to be performed | 
| Isokinetic strength test | Strength test | 24 hours after strenuous training session. Second test to be performed | 
| YO YO Intermittent Recovery Level test 1 | aerobic test | 24 hours after strenuous training session. Fourth test to be performed | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Probiotic, Gut damage, Sport performance