Phospholipid Absorption of a Milk Phospholipid

Study Overview
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Brief Summary
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The aim of the study is to investigate parameters of the lipid metabolism in plasma and feces of healthy subjects after a dietary supplementation of different dosages of milk phospholipids.

Detailed Description
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To date the mechanisms for the intestinal absorption of glycerophosphates and sphingolipids are not completely investigated. It is assumed, that sphingolipids and sterols compete for intestinal absorption. In addition it is unclear, if a dietary phospholipid supplementation may influence the phospholipid concentration in plasma. To evaluate the effect of a dietary phospholipid supplementation 15 healthy female subjects received over 10 days daily 3 g of milk phospholipids stirred in a dairy product. During the following 10 days the subjects were supplemented with daily 6 g milk phospholipid. Finally, the subjects received over 10 days 6 g of the milk phospholipid in a dairy product and 2 g of plant sterols serving a commercial product.

Official Title
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Effect of a Milk Phospholipid Supplementation on the Lipid Metabolism in Healthy Volunteers

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Milk phospholipid


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: female age: between 30 and 45 years Exclusion Criteria: metabolic diseases arteriosclerosis pregnancy and breast feeding

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Phospholipid supplementation<br>Supplementation of milk phospholipid | Dietary Supplement: Milk phospholipid<br>* During day 1 until 10 the subjects were supplemented with daily 3 g milk phospholipids, during the day 11 and 20 the supplementation was increased to daily 6 g, and during the day 21 and 30 the subjects received daily 6 g milk phospholipids and 2 g of plant sterols.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in plasma phospholipid concentration | Total phospholipid concentration Individual phospholipid concentration (lyso phosphatidylcholine, phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, phosphatidylinositol) | after 0, 10, 20, and 30 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma lipids | Fatty acid fraction (total fatty acid distribution, phospholipid fatty acid distribution) Cholesterol fraction (total cholesterol, LDL cholesterol, HDL cholesterol) Triacylglycerides fraction | after 0,10,20, and 30 days | 
| Fecal lipids | Fecal lipids (crude fat, total phospholipids, sterols, bile acids) | after 0,10,20, and 30 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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glycerophosphates, sphingomyelins