Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Level

Study Overview
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Brief Summary
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The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme activity by using intracrevicular and orifice methods.

Detailed Description
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Twenty adults (mean age of 41.8 years. ranged 31-60 years. 18 males. 2 females) with chronic periodontitis were enrolled and all completed the 3-day study. Three-hundred-sixty samples of GCF were harvested from the interproximal sites of each of the two maxillary non-molar teeth according to the orifice method. Each site was sampled three times with 1-min intervals between repeat samples. By using intracrevicular method, the other 360 samples of GCF were collected on the same sites following 10-minutes interval. In first day, 2nd day and 3rd day the length of sampling time in seconds and order were 5. 10. 30; 10. 30. 5 and 30. 5. 10. respectively. GCF elastase activity was determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide and was expressed as concentration (microU/microl) and total enzyme activity (microU).

Official Title
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Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Levels: Sequential Sampling, Length of Sampling Time And Two Different Sampling Methods

Conditions
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Chronic Periodontitis

Intervention / Treatment
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* Other: Gingival crevicular fluid collection with filter paper


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: no history of systemic disease; no history of antibiotics and/or anti-inflammatory drugs within the past 6 months; no received any oral hygiene prophylaxis and scaling and root planing within the past 6 months; at least 2 interproximal sites exhibiting higher than 5 mm probing depth, radiographic bone loss and gingival index of 2 (with preference of two maxillary non-molar teeth) Exclusion Criteria: with metabolic diseases pregnancy, lactation etc.

Ages Eligible for Study
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Minimum Age: 31 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: the first day<br>Gingival crevicular fluid collection with filter paper the length of sampling time in seconds: 5., 10., 30. | Other: Gingival crevicular fluid collection with filter paper<br>* Gingival Crevicular Fluid Dynamics<br>|
| Experimental: the 2nd day<br>Gingival crevicular fluid collection with filter paper the length of sampling time in seconds: 10., 30., 5. | Other: Gingival crevicular fluid collection with filter paper<br>* Gingival Crevicular Fluid Dynamics<br>|
| Experimental: the 3rd day<br>Gingival crevicular fluid collection with filter paper the length of sampling time in second: 30., 5., 10. | Other: Gingival crevicular fluid collection with filter paper<br>* Gingival Crevicular Fluid Dynamics<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The length of sampling time of GCF Elastase Levels |  | 3 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The length of sampling time of GCF Volume |  | 3 days | 
| The length of sequential sampling of GCF Volume |  | 3 days | 
| The length of sequential sampling of GCF Elastase Levels |  | 3 days |