Effects of Resistance Exercise on Metabolic Responses

Study Overview
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Brief Summary
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Healthy young adults will complete three trials in a randomized crossover counter-balanced order, including two different loads of resistance exercise and sedentary control. During each trial, blood samples will be collected. The investigators hypothesized that high-load and low-load resistance exercise exert similar metabolic responses.

Official Title
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Effects of Volume-matched Resistance Exercise With Different Loads on Metabolic Responses

Conditions
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Inflammatory Response

Intervention / Treatment
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* Other: High-load resistance exercise
* Other: Low-load resistance exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age: 20-30 Healthy male Without experience of resistance training Exclusion Criteria: No acute or chronic musculoskeletal symptoms Smoking Alcohol or drug abuse Fail to conduct resistance exercise

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: High-load resistance exercise<br>4 high-load resistance exercises that target all major muscle groups. | Other: High-load resistance exercise<br>* Subjects in high-load trial performed 4 sets per exercise, 8 repetitions with load of 85%-8RM with 90 sec of rest between sets.<br>|
| Experimental: Low-load resistance exercise<br>4 low-load resistance exercises that target all major muscle groups. | Other: Low-load resistance exercise<br>* Subjects in low-load trial performed 4 sets per exercise, 15 repetitions with load of 45%-8RM with 90 sec of rest between sets.<br>|
| No Intervention: Sedentary control<br>Subjects in control trial stayed sedentary during the period. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in glucose | glucose in mg/dL | 4-hour during each study intervention | 
| Changes in insulin | insulin in mU/L | 4-hour during each study intervention | 
| Changes in TG | TG mg/dL | 4-hour during each study intervention | 
| Changes in uric acid | uric acid mg/dL. | 4-hour during each study intervention | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in biomarkers of inflammation | Biomarkers of inflammation will be measured such as IL-10 in pg/mL and IL-6 in pg/mL. | 4-hour during each study intervention | 
| Changes in cortisol | cortisol in ng/mL | 4-hour during each study intervention | 
| Changes in lactate | lactate in mmol/L | 4-hour during each study intervention | 
| Changes in complete blood count | blood cell count in count per µL | 4-hour during each study intervention | 
| Borgs Ratings of Perceived Exertion (RPE) | Borgs RPE is a method of estimating exertion rating, based on a 6 to 20 rating scale (6 being the no exertion at all, 20 being maximal exertion). | 1-hour during each exercise intervention | 
| Changes in ET-1 | ET-1 in pg/mL | 4-hour during each study intervention | 
| Changes in NO | NO in µmol/L | 4-hour during each study intervention | 
| Changes in biomarkers of muscle damage | Biomarkers of muscle damage will be measured such as CK in U/L and LDH in U/L. | 4-hour during each study intervention | 
| Heart rate (HR) | HR will be measured during each study intervention. | 6-hour during each study intervention | 
| Visual Analogue Scale (VAS) of appetite | Hunger, fullness, desire to eat, and prospective food consumption will be measured via VAS ratings, based on a 100 mm long scale (0 being not at all, 100 being extremely). | 6-hour during each study intervention | 
| Changes in biomarkers of appetite | Biomarkers of appetite will be measured such as ghrelin in pg/mL and PYY in pg/mL. | 4-hour during each study intervention | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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resistance exercise, equal training volume, immune response