Salivary Antioxidant Capacity Before and After Complete Dental Rehabilitation

Study Overview
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Brief Summary
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Salivary samples were obtained from 20 children in their fifth year of life diagnosed S-ECC and 20 age and sex matched controls. Complete dental rehabilitation under general anesthesia was performed for the 20 children with S-ECC, and follow up salivary samples were obtained one week and three months post operatively. The TAC was measured using a commercially available Oxygen Radical Absorbance Antioxidant Assay measurement kit (Zen-Bio ORAC™, AMS Biotechnology, Abington UK).

Detailed Description
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Background: The association of total salivary antioxidant capacity (TAC) to severe early childhood caries (S-ECC) has been studied in the past. It is not clear if TAC is an indicator of the inflammatory response to the lesion or a marker of the disease. Aim: This study aimed to measure the salivary TAC levels in children with early childhood caries before and after dental treatment and compare the results to caries free children. Methodology: Salivary samples were obtained from 20 children in their fifth year of life diagnosed S-ECC and 20 age and gendersex matched controls. Complete dental rehabilitation under general anesthesia was performed for the 20 children with S-ECC, and follow up salivary samples were obtained one week and three months post operatively. The TAC was measured using a commercially available Oxygen Radical Absorbance Antioxidant Assay measurement kit (Zen-Bio ORAC™, AMS Biotechnology, Abington UK).

Official Title
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Salivary Antioxidant Capacity of Children With Severe Early Childhood Caries (S-ECC) Before and After Complete Dental Rehabilitation

Conditions
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Dental Caries in Children

Intervention / Treatment
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* Procedure: Dental rehabilitation under general anesthesia


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Medically fit children Age below 71 months Test group - to be diagnosed with severe ECC Control group - to be caries free Exclusion Criteria:

Ages Eligible for Study
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Minimum Age: 60 Months
Maximum Age: 72 Months

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| S-ECC<br>Children in their fifth year of life with severe early childhood caries Children selected will require complete dental rehabilitation under general anesthesia All enrolled children will receive treatment of all dental carious lesions under general anesthesia | Procedure: Dental rehabilitation under general anesthesia<br>* Dental treatment of all teeth affected by dental caries in a single visit under general anesthesia<br>|
| Control<br>Children age and sex matched with the S-ECC group who are free of dental caries | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in TAC after one week | Change in Total Salivary Antioxidant Capacity of children from baseline to one week after treatment | One week | 
| Change in TAC after Three months | Change in Total Salivary Antioxidant Capacity of children from baseline to three months after treatment | Three months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Antioxidant Capacity