DAnalysis of the Distribution of Adrenal Steroids in a Control Population

Study Overview
=================
Brief Summary
-----------------
This is a biomedical research study on the determination of adrenal steroids. It has been proposed in recent years as a tool for the diagnostic orientation of an adrenal pathology. The main objective is to analyze the distribution of blood and urinary adrenal steroid values in a population of 60 controls. The secondary objective is to establish reference values for each of these parameters, if their distribution permits, and to study the possible link between the blood pressure level, the orthostatism state, the potassium level and these adrenal steroids.

Official Title
-----------------
Analysis of the Distribution of Adrenal Steroids in Blood and Urine Measured by Mass Spectrometry Coupled to Liquid Chromatography in a Control Population

Conditions
-----------------
Adrenal Disease

Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Negative blood pregnancy test Normosalt diet Men and women ≥ 18 years Being able and willing to give written informed consent Exclusion Criteria: Personal history of diabetes, high blood pressure, adrenal pathologies. Family history of adrenal pathologies. Taking of the following drugs: Antihypertensives drugs: ACE inhibitor, Angiotensin II receptor, beta-blocker, thiazide diuretic, loop diuretic, anti-aldosterone, dihydropyridine-type calcium antagonist. Hormone replacement therapy, cyproterone acetate NSAID (except occasional intake), corticosteroid Antidepressant: serotonin reuptake inhibitor (paroxetine, fluoxetine, sertraline, citalopram) Contraception or estrogen / progestin treatment (progestins only allowed) Not be able to keep standing position.

Ages Eligible for Study
-----------------
Minimum Age: 18 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy subjects<br> | |

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| difference of the distribution of blood steroid values in a population control group | The value will be measured by mass spectrometry coupled with liquid chromatography (HPLC-MS) and 16 global parameters will be analyses (B, 18-OH B, DOC, 18-OH DOC, S, F, 18-OH F, 17-OH progesterone, 21-desoxycortisol, delta 4 androstenedione, 17OHpregnenolone, aldosterone, SDHA, E, testosterone, estradiol | At baseline | 
| difference of the distribution of urinary steroid values in a population control group | The value will be measured by mass spectrometry coupled with liquid chromatography (HPLC-MS) and 6 global parameters will be analyses (E, F, THF, aTHF, THE, aldosterone) | At baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood pressure taken while sitting, lying or standing |  | At baseline | 
| blood concentration of kaliemia |  | at baseline | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
Adrenal steroids, mass spectroscopy, liquid chromatography