Examining the Impact of Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males

Study Overview
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Brief Summary
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This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.

Official Title
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Examining the Impact of Acute Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males: A Randomized Cross-over Trial

Conditions
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Mitochondrial Biogenesis

Intervention / Treatment
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* Other: BFR Exercise
* Other: CTL Exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 3 hours of physical activity per week No previous cycling training No concurrent involvement in another training program Body mass index < 30 kg/m2 Exclusion Criteria: Presence of cardiovascular disease Presence of metabolic disease Taking regular oral medication Current smoker

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: CTL - BFR<br>CTL Exercise then BFR Exercise | Other: BFR Exercise<br>* Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).<br>Other: CTL Exercise<br>* Participants will exercise on a recumbent bike placed on flat ground (CTL).<br>|
| Experimental: BFR - CTL<br>BFR Exercise then CTL Exercise | Other: BFR Exercise<br>* Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).<br>Other: CTL Exercise<br>* Participants will exercise on a recumbent bike placed on flat ground (CTL).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) | Fold-change in PGC-1α mRNA expression | 15 minutes before exercise and 3 hours post-exercise for both exercise sessions | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Catecholamine concentrations | Catecholamine concentrations in venous blood | 15 minutes before exercise and immediately post-exercise for both exercise sessions | 
| Total and phosphorylated p38 mitogen-activated protein kinase (MAPK) | Fold change in p38 MAPK phosphorylation | 15 minutes before exercise and immediately post-exercise for both exercise sessions | 
| Total and phosphorylated acetyl coenzyme A carboxylase (ACC) | Fold change in ACC phosphorylation | 15 minutes before exercise and immediately post-exercise for both exercise sessions | 
| Total and phosphorylated protein kinase A (PKA) | Fold change in PKA phosphorylation | 15 minutes before exercise and immediately post-exercise for both exercise sessions | 
| Acetylated p53 | Fold change in acetylated p53 | 15 minutes before exercise and immediately post-exercise for both exercise sessions | 
| Blood lactate | Average [lactate] between conditions | During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions | 
| Heart rate (HR) | Average HR between conditions | During both exercise sessions | 
| Revolutions per minute (RPM) | Average RPM between conditions | During both exercise sessions | 
| Ratings of perceived exertion (RPE) | Average RPE between conditions | During both exercise sessions | 
| Oxygenated and deoxygenated hemoglobin | Average oxygenated and deoxygenated hemoglobin as a percentage of same day max values | During both exercise sessions and up to 10 minutes after (for cuff occlusion) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Acute Exercise, Mitochondria, Skeletal Muscle, PGC1α, Mitochondrial Biogenesis, Intracellular Signalling