Epigenetic Regulation of Immunity in Alpha-1 Anti-trypsin Deficiency

Study Overview
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Brief Summary
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The investigators hypothesize that environmentally influenced histone modifications regulate AM mediated inflammation, contributing to a variable clinical course of AATD, and may also influence or be influenced by the activity of AAT augmentation therapy.

Detailed Description
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The variable natural clinical course of alpha-1 anti-trypsin deficiency (AATD) disease and strong influence of environmental exposures such as smoking, implicate a major role for epigenetic mechanisms in modifying AATD disease penetrance. The goal of this study proposal is to investigate epigenetic regulation of alveolar macrophage (AM) inflammation and function in AATD PiZZ (two Z genes) and PiMZ (one M and one Z gene) patients. The investigators proposal focuses on epigenetic histone modifications and gene expression specifically in AM. AAT augmentation therapy, which alters disease symptoms, may also modulate AM epigenetics. To identify epigenetic regulation of AM inflammation in AATD in the context of AAT therapy, the investigators will perform and computationally integrate ChIP-seq and RNA-seq data. This will help elucidate the immunomodulatory mechanisms regulating AATD and provide an epigenetic map for diagnosis and targeted treatment. The investigators will test the efficacy of FDA-approved histone modifying drugs, such as Suberoylanilide Hydroxamic Acid (SAHA) and more specific next-generation histone modifiers, such as GSK-J4, to modulate AM AATD-associated activity ex vivo. The goal of this study is to enroll up to a total of 13 AATD cases and 6 healthy controls. All AATD patients will be asked to give a blood sample and/or undergo a bronchoscopy. AATD patients will also be asked to undergo a follow up bronchoscopy and/or blood draw after 6 months if treatment with alpha-1 antitrypsin augmentation therapy is initiated to study the changes in these markers after augmentation therapy.

Official Title
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Defining Epigenetic Regulation of Immunity in Alpha-1 Anti-trypsin Deficiency

Conditions
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Alpha 1-Antitrypsin Deficiency

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: AATD Age between the ages of 18 and 85 Have a diagnosis of AATD PiZZ or PiMZ established by AAT blood levels and Pi genotyping Are not and have not been on AAT augmentation therapy for the past 6 months Able to tolerate and willing to undergo study procedures Provide signed informed consent. Exclusion Criteria: AATD History of comorbid condition severe enough to significantly increase risks based on investigator discretion Diagnosis of unstable cardiovascular disease including myocardial infarction in the past 6 weeks, uncontrolled congestive heart failure, or uncontrolled arrhythmia Partial Pressure of Oxygen (PaO2) on room air at rest <50 mmHg or Oxygen saturation (SaO2) on room air at rest <85% Post bronchodilator Forced expiratory volume in one second (FEV1)<30% predicted Use of anticoagulation (patients on warfarin or clopidogrel will be excluded; patients on aspirin alone can be studied even with concurrent use) Dementia or other cognitive dysfunction which in the opinion of the investigator would prevent the participant from consenting to the study or completing study procedures Active pulmonary infection with tuberculosis History of pulmonary embolism in the past 2 years Non-Chronic Obstructive Pulmonary Disease (COPD) obstructive disease (various bronchiolitides, sarcoid, lymphangioleiomyomatosis (LAM), histiocytosis X) or parenchymal lung disease, pulmonary vascular disease, pleural disease, severe kyphoscoliosis, neuromuscular weakness, or other cardiovascular and pulmonary disease, that, in the opinion of the investigator, limit the interpretability of the pulmonary function measures Prior significant difficulties with pulmonary function testing Hypersensitivity to or intolerance of albuterol sulfate or ipratropium bromide or propellants or excipients of the inhalers Hypersensitivity to or intolerance of all drugs required for sedation during conscious sedation bronchoscopy. History of Lung volume reduction surgery, lung resection or bronchoscopic lung volume reduction in any form History of lung or other organ transplant History of large thoracic metal implants (e.g., Implantable cardioverter-defibrillator (AICD) and/or pacemaker) that in the opinion of the investigator limit the interpretability of CT scans Currently taking >=10mg a day/20mg every other day of prednisone or equivalent systemic corticosteroid Currently taking any immunosuppressive agent excepting systemic corticosteroids History of lung cancer or any cancer that spread to multiple locations in the body Current illicit substance abuse, excluding marijuana Known HIV/AIDS infection History of or current exposure to chemotherapy or radiation treatments that, in the opinion of the investigator, limits the interpretability of the pulmonary function measures. Has a BMI > 40 kg/m2 at baseline exam Current or planned pregnancy within the study course. Currently institutionalized (e.g., prisons, long-term care facilities) Have a genotype of PiMZ and ever received intravenous or inhaled alpha-1 augmentation therapy (Alpha-1 Proteinase Inhibitor, A1PI) Conditional Exclusions Participants who present with an upper respiratory infection or pulmonary exacerbation, either solely participant-identified or that has been clinically treated, in the last six weeks can be rescreened for the study once the six-week window has passed. Participants who present with current use of acute antibiotics or steroids can be rescreened for the study ≥30 days after discontinuing acute antibiotics/steroids. This does not apply to participants who are on chronic prednisone therapy of <10 mg per day or <20 mg every other day. Participants who present with a myocardial infarction or eye, chest, or abdominal surgery within six weeks can be rescreened after the six week window has passed. Study coordinators should consult with the site principal investigator prior to rescreening these participants. Female participants who present <3 months after giving birth will be asked to reschedule their visit until three months have passed since the birth. Individuals who are PiZZ receiving alpha-1 augmentation therapy (Alpha-1 Proteinase Inhibitor, A1PI) must be off augmentation therapy for >6 months to qualify for stratified enrollment in the PiZZ group not receiving augmentation therapy.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Alpha-1 Antitrypsin<br>All AATD patients who will start treatment with alpha-1 antitrypsin augmentation therapy | |
| Healthy Control<br>Healthy controls with no lung diseases | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Epigenetic signature of specific inflammation-associated histone modifications from CD14+ macrophages |  | Change from Baseline histones at 6 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Epigenetically regulated genomic profile of AATD in AM |  | Change from Baseline polyA RNA at 6 months | 
| Epigenetic mechanisms to regulate gene expression and cell function |  | Change from Baseline epigenetic modified cells at 6 months |