Metabolic Phenotypes in Childhood Obesity

Study Overview
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Brief Summary
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This study provides a sound basis towards a better understanding of the biochemical mechanisms behind childhood obesity and its metabolic phenotypes, which will be of great importance towards the development of more personalized prevention and treatment in future.

Detailed Description
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The aim of this study was to identify the plasma metabolic profile associated with childhood obesity and its phenotypes.. This cross-sectional study was set-up to examine the metabolic profile in fasting plasma samples from 65 overweight and obese (OB) children and 37 normal-weight (NW) controls aged 8 to 18 years. The obtained plasma 1H-NMR spectra were rationally divided into 110 integration regions, representing the metabolic phenotype. These integration regions reflect the relative metabolite concentrations and were used as statistical variables to construct a classification model in discriminating between OB and NW children and adolescents. In addition, two metabolic phenotypes were defined within the OB group, i.e. the metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) phenotype which are both classified as obese but with respectively, none (MHO) or at least two components (MUO) of the metabolic syndrome.

Official Title
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Identification of Metabolic Phenotypes in Childhood Obesity by 1H-NMR Metabolomics of Blood Plasma

Conditions
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Obesity, Pediatric

Intervention / Treatment
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* Other: Blood sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: aged 8 to 18 normal-weight, overweight or obese according to the International Obesity Task Force (IOTF) Body Mass Index (BMI) criteria fasted for at least 8 hours Exclusion Criteria: Subjects taking any medication or having serious chronic or acute illness within two weeks preceding the clinical examination,

Ages Eligible for Study
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Minimum Age: 8 Years
Maximum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| OB-NMR<br>overweight and obese children and adolescents | Other: Blood sampling<br>* Determine the metabolic phenotype of blood plasma by proton-NMR spectroscopy<br>|
| NMR-C<br>normal-weight children and adolescents | Other: Blood sampling<br>* Determine the metabolic phenotype of blood plasma by proton-NMR spectroscopy<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| metabolic phenotype of childhood obesity | Significant metabolic changes in blood plasma of obese children and adolescents and normal-weight control subjects | day 1 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| metabolic phenotype of metabolically healthy and unhealthy obese children and adolescents | Significant metabolic changes in blood plasma of metabolically healthy obese and metabolically unhealthy obese children and adolescents | day 1 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Metabolic syndrome, Metabolomics, NMR spectroscopy