Distribution of Smooth Muscle In Dartos In The Non Conspicuous Penis

Study Overview
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Brief Summary
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Non-conspicuous penis (congenital megaprepuce, occult penis) is a symptomatic malformation that includes phimosis and excessively baggy, urine-filled prepuce with alteration of the appearance of the penis. A redundant and enlarged foreskin is the main feature of this entity.This congenital anomaly is difficult to diagnose and may have association with other pathologies such as buried penis. Currently, part of the megaprepuce skin is used to correct the defect. A recent study shows that patients with this pathology and hypospadias present mostly defects in the muscle dartos. The investigators do not know the physiological bases of the megaprepuce, neither the clinical and aesthetics implications of this abnormal tissue for the patient, and how this affects the postoperative evolution. With the present study the investigators intended to answer these questions and to open paths for future research in this area.

Detailed Description
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After informed consent, patients will be labeled with a research record number other than the identity document or the number of the attention. The urologist who perform the correction of the megaprepuce, will take a segment of the dartos and send it for histopathological analysis: staining with hematoxylin-eosin, smooth muscle actin marker, associated with the research record number. Same procedure will be performed with the controls and hypospadias group. Only the principal investigator will know the assignment of medical record and their respective group (congenital megaprepuce, hypospadias or controls). The samples will be sent to pathology without any clinical data, and they will be analyzed by two pathologists, both blind.

Official Title
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Distribution of Smooth Muscle In Dartos In The Non Conspicuous Penis, an Observational a Prospective, Descriptive, Observational Study

Conditions
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Congenital Urogenital Anomaly

Intervention / Treatment
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* Diagnostic Test: Hematoxylin-eosin and smooth muscle actin markers


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male patients scheduled for correction of congenital megaprepuce Male patients scheduled for correction of hypospadias Male patients scheduled for circumcision for non-medical reasons. Exclusion Criteria: Patients with a history of hypospadias correction.

Ages Eligible for Study
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Minimum Age: 1 Month
Maximum Age: 10 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Congenital Megaprepuce<br>Congenital Megaprepuce Hematoxylin-eosin and smooth muscle actin markers | Diagnostic Test: Hematoxylin-eosin and smooth muscle actin markers<br>* The foreskin arrives oriented in a single piece in formol to the unit of pathology, and in the pathology unit, they must: Measure the length, width and thickness of the specimen. The specimen should include skin and mucosa with the underlying areolar tissue. Examine the surfaces of the sample searching lesions, and describe them in size, appearance (warty, papillary, ulcerated), depth of invasion, and distance from the nearest cutting edge, if they are present.<br>|
| Hypospadias<br>Hypospadias Hematoxylin-eosin and smooth muscle actin markers | Diagnostic Test: Hematoxylin-eosin and smooth muscle actin markers<br>* The foreskin arrives oriented in a single piece in formol to the unit of pathology, and in the pathology unit, they must: Measure the length, width and thickness of the specimen. The specimen should include skin and mucosa with the underlying areolar tissue. Examine the surfaces of the sample searching lesions, and describe them in size, appearance (warty, papillary, ulcerated), depth of invasion, and distance from the nearest cutting edge, if they are present.<br>|
| Control<br>Circumcision for non-medical reasons. Hematoxylin-eosin and smooth muscle actin markers | Diagnostic Test: Hematoxylin-eosin and smooth muscle actin markers<br>* The foreskin arrives oriented in a single piece in formol to the unit of pathology, and in the pathology unit, they must: Measure the length, width and thickness of the specimen. The specimen should include skin and mucosa with the underlying areolar tissue. Examine the surfaces of the sample searching lesions, and describe them in size, appearance (warty, papillary, ulcerated), depth of invasion, and distance from the nearest cutting edge, if they are present.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Differences in the distribution of the smooth Muscle In Dartos | Smooth Muscle Fibers | 1 year | 
| Describe the pattern of smooth muscle in patients with megaprepuce | Smooth Muscle Fibers | 1 year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Describe the pattern of smooth muscle in patients with hypospadias | Smooth Muscle Fibers | 1 year | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Congenital Abnormalities, Hypospadias, Congenital megaprepuce, Histology