Subgingival Microbiota Following Mechanical Treatment and it's Effect on Peri-implant Sub-mucosa in Periodontitis Patients

Study Overview
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Brief Summary
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The investigators planed to observe the microbiome of an aggressive periodontitis patient, during mechanical treatment, surgery(if indicated) and implant placement. The investigators designed to observe the changes after mechanical treatment.Then, the investigators will compare the sub-mucosa microbiome of implant and the subgingival community. The investigators expected a different community after treatment, and there could be similarity between implant and nature teeth.

Official Title
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Subgingival Microbiome Following Mechanical Treatment and it's Effect on Peri-implant Sub-mucosa in Aggressive Periodontitis Patients

Conditions
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Aggressive Periodontitis, Generalized

Intervention / Treatment
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* Procedure: mechanical treatment(mainly scaling and root planing), periodontal surgery(if indicated), implant placement.


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subjects were between 18-35 years old and diagnosed with generalized aggressive periodontitis (GAgP) according to the 1999 International Classification of Periodontal Diseases (Armitage 1999); At least 20 teeth in the first appointment and at least 3 teeth(other than first molars and central incisors)with a probing depth(PD) ≥ 6mm, clinical attachment level (CAL)≥5mm; The clinical diagnosis was confirmed by evidence of inter-proximal bone loss on full-mouth periapical radiographs; Family history of aggressive periodontitis. Exclusion Criteria: Uncontrolled systemic conditions including diabetes and hypertension; Pregnant or lactating; Smoking; Received periodontal treatment within the previous 6 months or antibiotic medication during the previous 3 months.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Procedure: mechanical treatment(mainly scaling and root planing), periodontal surgery(if indicated), implant placement.|The investigators shall follow the regular procedure to these patients.|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| changes of diversity of subgingival community (compare the sub-mucosa microbiome of implant and the subgingival community) | the diversity of subgingival community before and after mechanical treatment | Baseline, 6 weeks after mechanical treatment | 
| changes of diversity of subgingival community (compare the sub-mucosa microbiome of implant and the subgingival community) | the diversity of submucosa community before and after implatation | before implantation, 1 months after second stage surgery, and every month after prosthetic treatment until 2 years. | 
| changes of prevalence and abundance of OTUs | compute and compare the prevalence and abundance of OTUs | Baseline, 6 weeks after mechanical treatment | 
| changes of prevalence and abundance of OTUs | compute and compare the prevalence and abundance of OTUs | before implantation, 1 months after second stage surgery, and every month after prosthetic treatment until 2 years. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| changes of bleeding index | bleeding index after probing | Baseline, 6 weeks after mechanical treatment, before implantation, 1 months after second stage surgery, every month after prosthetic treatment until 2 years. | 
| changes of probing depth | probing depth of indicated sites | Baseline, 6 weeks after mechanical treatment, before implantation, 1 months after second stage surgery, every month after prosthetic treatment until 2 years. | 
| clinical attachment loss | by probe, the distance between CEJ and gingival sulcus | Baseline, 6 weeks after mechanical treatment, before implantation, 1 months after second stage surgery, every month after prosthetic treatment until 2 years. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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mechanical treatment, implant