Glycemic and Insulinemic Response of Pasta Products in Comparison to Asian Foods

Study Overview
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Brief Summary
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The aim of the study is to determine the glycaemic and insulinaemic index of pasta products and local foods. It is hypothesized that different pasta cuts and wholegrain composition of pasta and local foods have different GI depending on the nature of the carbohydrate content and the food structure within each food.

Detailed Description
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There will be a total of 9 test sessions and each session will last up to 3.5 hours. The order of sessions will be determined with an online randomizer. At each test session, the following procedures will be performed: two finger-prick blood samples, five minutes apart to measure baseline blood glucose and insulin concentrations Subject will be served the test food and to consume within15 minutes Following the test meal, blood samples will be collected at the following time points: 15, 30, 45, 60, 90, 120, 150 and 180min for glucose measurements and insulin measurements will be collected at 30, 60, 90, 120, 150 and 180min.

Official Title
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Glycemic and Insulinemic Response of Pasta Products in Comparison to Asian

Conditions
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Diabetes

Intervention / Treatment
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* Other: Glucose Reference 1
* Other: Glucose Reference 2
* Other: Glucose Reference 3
* Other: Semolina Penne
* Other: Wholegrain Penne
* Other: Semolina Spaghetti
* Other: Wholegrain Spaghetti
* Other: Jasmine Rice
* Other: Asian Noodles


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy Asian - Indian and Chinese ethnicity Age between 21-40 years Body mass index between 18.5 to 25 kg/m2 Normal blood pressure (<140/90 mmHg) Fasting blood glucose < 6 mmol/L Exclusion Criteria: Is a current smoker Is currently pregnant Have any metabolic diseases (such as diabetes, hypertension etc) Have known glucose-6-phosphate dehydrogenase deficiency (G6PD deficiency) Have medical conditions and/or taking medications known to affect glycemia(glucocorticoids, thyroid hormones, thiazide diuretics) Have any major organ dysfunction (e.g. cardiovascular, respiratory, hepatic, renal, gastrointestinal) that may influence taste, olfaction, appetite, digestion, metabolism, absorption or elimination of test foods, nutraceutical or drug Have any severe food allergy (e.g. anaphylaxis to peanuts) Have any known allergies to any food components of the study protocol Have active Tuberculosis (TB) or currently receiving treatment for TB Have any known Chronic infection or known to suffer from or have previously suffered from or is a carrier of Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Human Immunodeficiency Virus (HIV) Is a study team member or an immediate family of any study team member. Immediate family member is defined as a spouse, parent, child, or sibling, whether biological or legally adopted. enrolled in a concurrent research study judged not to be scientifically or medically compatible with the study. Have partake in sports at the competitive and/or endurance levels Have intentionally restrict food intake

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Glucose Reference 1<br>50g of glucose dissolved in 250ml of water to be consumed. | Other: Glucose Reference 1<br>* Glucose contains 50g of available carbohydrates<br>|
| Other: Glucose Reference 2<br>50g of glucose dissolved in 250ml of water to be consumed. | Other: Glucose Reference 2<br>* Glucose contains 50g of available carbohydrates<br>|
| Other: Glucose Reference 3<br>50g of glucose dissolved in 250ml of water to be consumed. | Other: Glucose Reference 3<br>* Glucose contains 50g of available carbohydrates<br>|
| Experimental: Semolina Penne<br>70g of semolina penne boiled in 1 Litre of water for 11 minutes. Drained and consume immediately. | Other: Semolina Penne<br>* Semolina Penne contains 50g of available carbohydrates<br>|
| Experimental: Wholegrain Penne<br>76g of wholegrain penne boiled in 1 Litre of water for 9 minutes. Drained and consume immediately. | Other: Wholegrain Penne<br>* Wholegrain Penne contains 50g of available carbohydrates<br>|
| Experimental: Semolina Spaghetti<br>71g of semolina spaghetti boiled in 1 Litre of water for 8 minutes. Drained and consume immediately. | Other: Semolina Spaghetti<br>* Semolina Spaghetti contains 50g of available carbohydrates<br>|
| Experimental: Wholegrain Spaghetti<br>76g of wholegrain spaghetti boiled in 1 Litre of water for 8 minutes. Drained and consume immediately. | Other: Wholegrain Spaghetti<br>* Wholegrain Spaghetti contains 50g of available carbohydrates<br>|
| Experimental: Jasmine rice<br>63g of rice cook with 150g of water. Served and consume immediately. | Other: Jasmine Rice<br>* Jasmine Rice contains 50g of available carbohydrates<br>|
| Experimental: Asian Noodles<br>92.4g of Asian Noodles cook in boiling water for 45 seconds. Drained and consume immediately | Other: Asian Noodles<br>* Asian Noodles contains 50g of available carbohydrates<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glucose | Blood will be collected and measured using hemocue analyzer. | up to 180 minutes | 
| Insulin | Blood will be collected and measured using COBAS analyzer. | up to 180 minutes |