Resistance Training Effects on Gut Microbiome and Cardiometabolic Outcomes

Study Overview
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Brief Summary
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The overall objective of this study is to investigate the effects of resistance training on the gut microbiome and cardiometabolic outcomes in adults with overweight and obesity.

Detailed Description
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The overall objective of this study is to investigate the effects of resistance training on the gut microbiome and cardiometabolic outcomes in adults with overweight and obesity.

Official Title
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Effects of 6-week Resistance Training Intervention on Gut Microbiome and Cardiometabolic Outcomes in Adults With Overweight and Obesity

Conditions
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Exercise Intervention

Intervention / Treatment
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* Behavioral: Resistance training
* Other: Conventional physical activity guidelines


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 18-35 years BMI: 25-45 kg/m2 Sedentary lifestyle Consistent diet patterns Weight-stable (<4 kg change over the last 3 mo) Willingness to adhere to resistance training protocol and not change diet Non-smoker >1 year or more Exclusion Criteria: Disabilities preventing adherence to resistance training protocol Recent start of medications that affect metabolism or appetite Antibiotics Diabetes Drug therapy for coronary artery disease, peripheral artery disease, congestive heart failure, or dyslipidemia Uncontrolled hypertension and blood pressure ≥ 180/110 Gastrointestinal disease and/or bariatric surgery Smoking Illicit drug use Pregnant or lactating individuals

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Conventional physical activity guidelines<br>Participants are given recommendations on physical activity guidelines | Other: Conventional physical activity guidelines<br>* Participants are given recommendations on physical activity guidelines<br>|
| Experimental: Resistance training<br>Participants will undergo 6 weeks of supervised resistance training | Behavioral: Resistance training<br>* Participants will undergo 6 weeks of supervised resistance training<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in stool microbiome diversity | 16s rRNA sequencing | Change over 6 weeks | 
| Change in stool microbiome composition | 16s rRNA sequencing | Change over 6 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in blood pressure | diastolic and systolic blood pressure | change over 6 weeks | 
| Fat mass | body composition | Change over 6 weeks | 
| Fat free mass | body composition | Change over 6 weeks | 
| Change in fasting glucose | Fasting Glucose | change over 6 weeks | 
| Change in glucose regulation | 2 hour glucose clearance | change over 6 weeks | 
| Hip circumference | anthropometrics | change over 6 weeks | 
| Waist circumference | anthropometrics | change over 6 weeks | 
| Physical activity | kcalories burned during free living activity measured by accelerometer | change over 6 weeks | 
| Sleep Quality | Measured using actigraphs | change over 6 weeks | 
| Sleep Duration | Measured using actigraphs | change over 6 weeks | 
| 24hr appetite ratings | Subjective rating using visual analog scales | Once at baseline and at the end of 6 week intervention | 
| Change in Body mass | Measured in kg | change over 6 weeks | 
| Short chain fatty acids | Acetate, propionate, and butyrate | Change over 6 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Gut Microbiome, Resistance Training, Physical Activity