Effect of Amino Acid on Muscle Damage

Study Overview
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Brief Summary
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The primary objective is to determine the impact of essential amino acids on integrated muscle protein synthesis over 4 days after resistance exercise. In addition, the secondary objective is to determine the effect of essential amino acids on satellite cell regulation and inflammatory responses during this prolonged recovery period.

Official Title
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Effect of Leucine-enriched Essential Amino Acid on Integrated Muscle Protein Synthesis and Muscle Recovery After Resistance Exercise

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Active
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy, male, recreationally-active participants Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix. B). Active will be defined as being in the top 20% of age specific scores on iPAQ (the International Physical Activity Questionnaire; Appendix.C). Participants are required not to engage in lower limb RT (resistance training) for at least 6 months prior to the study. Participants will be 18-35 years old. Participants are willing to abide by the compliance rules of this study Exclusion Criteria: Female Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor Self-reported regular tobacco use Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.) The individual who has allergy for milk

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Placebo<br>4g of maltodextrin | Dietary Supplement: Placebo<br>* 4g of maltdextrin<br>|
| Active Comparator: Active<br>4g of essential amino acids | Dietary Supplement: Active<br>* 4g of essential amino acids<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein synthesis | Skeletal muscle fractional synthesis rate (%/h) will be determined by oral pulse method using ring-[D5]phenylalanine | 4 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| exercise performance | Maximal strength (torque in n-M) of the knee extensors will be assessed by a maximal isometric voluntary contraction (MVC). | 4 days | 
| Muscle soreness | Corresponding subjective ratings of muscle soreness will be collected from participants using a visual analogue scale (mm) | 4 days | 
| Muscle damage | Muscle damage will be determined by histochemical analysis. muscle damage is defined as Z-band streaming. | 4 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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exercise, recovery, muscle, protein