The Effect of Dietary Nitrate Supplementation on Linear Running Sprint Performance, Agility and Vertical Jump Performance in a Rested and Fatigued State

Study Overview
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Brief Summary
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Dietary nitrate (NO3-) supplementation has been shown to improve skeletal muscle contractility and fatigue resistance, particularly in fast-twitch (type II) muscle fibers. Furthermore, NO3- supplementation has been shown to speed reaction time. Taken together, these findings suggest that NO3- ingestion may be ergogenic during all-out sprint running and reactive agility tasks. Moreover, increasing muscle force production at high contraction speeds would be expected to translate into improved muscle power and, subsequently, improved performance in very explosive forms of exercise, such as vertical jumping. Therefore, the purpose of this investigation is to assess the effect of short term (5 days) NO3- supplementation on linear running sprint performance, reactive agility and vertical jump performance in a rested and fatigued state.

Official Title
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The Effect of Dietary Nitrate Supplementation on Linear Running Sprint Performance, Agility and Vertical Jump Performance in a Rested and Fatigued State

Conditions
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Exercise Performance

Intervention / Treatment
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* Other: Beetroot
* Other: Placebo beverage


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male and female participants in self-reported good general health as assessed by the standard procedures described below and specifically meeting normal blood pressure range (systolic: 100-135, diastolic: 60-90) and body mass index (BMI) range (16.0-29.9) Range of 18-45 years of age Participants must participate in an intermittent exercise based sport (e.g. soccer, rugby, hockey), at a competitive level (i.e. competes in officiated matches/games). Understanding of the procedures to be undertaken as part of the study Willingness to participate in exercise testing and follow supplementation guidelines and other instructions provided by the experimenter Informed, voluntary, written consent to participate in the study Exclusion Criteria: Known pulmonary, cardiovascular or metabolic disease Food allergies including phenylketonurea (PKU) Current regular use of any dietary supplement (excluding macronutrient based supplements) or previous use of any dietary supplement in the past 6 months that is known to have a lasting effect. Blood donation within 3 months prior to the start of the study Substance abuse within 2 years of the start of the study Smoking Any prescription and non-prescription medication which may impact saliva production (antidepressants, diuretics, analgesics, antihistamines, anti-hyper/hypo-tensives, anti-anxiety, appetite suppressants). Current or previous use of phosphodiesterase 5 (PDE5) inhibitors. Participation in another clinical trial within past 4 weeks.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Test Beet shot<br>Containing 385 mg nitrate | Other: Beetroot<br>* Containing 385 mg nitrate<br>|
| Placebo Comparator: Placebo beverage<br><0.1 mmol nitrate in sucrose solution with beet coloring | Other: Placebo beverage<br>* <0.1 mmol nitrate in sucrose solution with beet coloring<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Linear running sprint time | Linear running sprint in a rested and fatigued state | 5 days |