Quantitation of Bacteria Associated With Severe Periodontitis by Real -Time PCR Following Application of Diode Laser

Study Overview
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Brief Summary
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Primary objective To measure the gingival crevicular levels of A.actinomycetemcomitans and P.gingivalis after intra-pocket application of diode laser in severe periodontitis (stage 3 grade C) patients. Secondary objective To compare the levels of the same microorganisms as in cases of diode laser therapy to those after conventional treatment with systemic antibiotic administration in severe periodontitis (stage 3 grade C) patients. to measure clinical parameters probing depth,attachment loss and mobility grade

Official Title
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Quantitave Assessment of Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis by Real -Time PCR Following Application of Diode Laser in Severe Periodontitis (A RANDIOMIZED CONTROLLED CLINICAL TRIAL)

Conditions
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Periodontal Pocket

Intervention / Treatment
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* Device: medency diode laser
* Drug: spirazole forte


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients of both sexes having severe periodontitis (stage 3 grade C) with interdental clinical attachment loss (CAL) ≥ 5. Patients' age between 15 and 35 years old with rapid bone loss showed by radiograph. Systemically healthy patients. Radiographic bone loss extending to middle or apical third of root. Probing depth (PD) ≥ 6 mm. Exclusion Criteria: Use of antibiotics during the last 3 months. Pregnancy. Any systemic condition that might affect the study. Use of mouthwash containing antimicrobials during the previous 3 weeks. Smoking.

Ages Eligible for Study
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Minimum Age: 15 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: test group<br>intra pocket diode laser application | Device: medency diode laser<br>* intra pocket application of diode laser<br>|
| Active Comparator: control group<br>antibiotic adminstration | Drug: spirazole forte<br>* adminstration of spirazole forte with a dose 17 mg per kg<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Quantitation of Aggrecatibacter .actinomycetemcomitans and porphyromonas gingivalis bacteria | To measure the gingival crevicular levels of A.actinomycetemcomitans and P.gingivalis after intra-pocket application of diode laser in severe periodontitis(stage 3 grade C) patients | baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| comparing levels of bacteria between test and control groups | To compare the levels of the same microorganisms as in cases of diode laser therapy to those after conventional treatment with systemic antibiotic administration in severe periodontitis ( stage 3 grade C) patients. | baseline |