Bananas as an Energy Source During Exercise: a Metabolomics Approach

Study Overview
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Brief Summary
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This study compared the acute effect of ingesting bananas (BAN) versus a 6% carbohydrate drink (CHO) on 75-km cycling performance and post-exercise inflammation, oxidative stress, and innate immune function using traditional and metabolomics-based profiling.

Detailed Description
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Trained cyclists (N=14) completed two 75-km cycling time trials (randomized, crossover) while ingesting BAN or CHO (0.2 g/kg carbohydrate every 15 min). Pre-, post-, and 1-h-post-exercise blood samples were analyzed for glucose, granulocyte (GR) and monocyte (MO) phagocytosis (PHAG) and oxidative burst activity, nine cytokines, F2-isoprostanes, ferric reducing ability of plasma (FRAP), and metabolic profiles using gas chromatography-mass spectrometry.

Official Title
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Comparison of Bananas and Gatorade on Performance and Exercise-induced Inflammation, Oxidative Stress, and Changes in Immune Function in Endurance Athletes

Conditions
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Intense Exercise

Intervention / Treatment
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* Dietary Supplement: Bananas versus 6% carbohydrate beverage


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male Ages 18-45 years Cyclists, road racing experience. Willing to avoid the use of supplements, herbs, medications during the study. Exclusion Criteria: Female Over the age of 45 years No cycling racing experience Using supplements and medications during the study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Bananas versus 6% carbohydrate beverage<br> | Dietary Supplement: Bananas versus 6% carbohydrate beverage<br>* Trained cyclists (N=14) completed two 75-km cycling time trials (randomized, crossover) while ingesting BAN or CHO (0.2 g/kg carbohydrate every 15 min).<br>* Other names: Gatorade;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Performance and fuel substrate utilization as measured through metabolomics | Will measure shifts in metabolites following 75-km cycling while ingesting bananas or 6% carbohydrate beverage. The shift in metabolites will be assessed using GC-MS. Time frame from date of randomization until the last samples are collected and analyzed will be up to six months. | Up to 6 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Exercise-induced inflammation. | Cytokine panel (IL-6, IL-8, IL-10, TNF-alpha) before and after cycling 75-km; measured with Meso Scale Discovery in pg/ml. Time frame from randomization until the last samples are collected and analyzed will be up to six months. | up to 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Bananas, Exercise, Metabolomics, Oxidative stress, Inflammation, Immune function