Breaking Sitting and Metabolic Health in Sedentary Overweight and Obese Adults

Study Overview
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Brief Summary
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The purpose of this project is to investigate the effects of breaking up prolonged sitting on postprandial metabolic responses, gut hormones secretion and energy balance in sedentary overweight and obese adults.

Detailed Description
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Two trials are included in this project - Prolonged sitting and Breaking sitting. In the Prolonged sitting trial, after consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min. In the Breaking sitting trial, after consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min. Blood samples were collected in a regular pattern throughout the trials. Energy balance (e.g., energy intake and spontaneous physical activity) was assessed using food diary and an accelerometer until midnight in the trial days once participants left laboratory.

Official Title
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Acute Effects of Breaking Prolonged Sitting on Postprandial Glycaemic Response, Incretin Hormones Secretion and Eating Behaviour in Overweight and Obese Adults

Conditions
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Metabolic Health, Gut Hormones, Energy Balance

Intervention / Treatment
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* Behavioral: Prolonged sitting
* Behavioral: Breaking sitting


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Physical inactive males and females Aged between 20 and 45 years Overweight and obese healthy adults (waist circumference greater than 90 cm for males and 80 cm for females) Weight stable for more than 3 months (no change in weight +/- 3%) Non-smoker Able to walk comfortably on a treadmill Exclusion Criteria: Personal history of/existing diabetes, cardiovascular disease, metabolic disease or dyslipidaemia Waist circumference < 90 cm for male and < 80 cm for female Taking medications that may influence lipid or carbohydrate metabolism or immune system function Unable to take part in exercise for any reason (e.g., injury or disability) or a positive response to any questions on the Physical Activity Readiness questionnaire (PAR-Q)

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Prolonged sitting<br>Prolonged sitting | Behavioral: Prolonged sitting<br>* After consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min.<br>|
| Experimental: Breaking sitting<br>Breaking prolonged sitting | Behavioral: Breaking sitting<br>* After consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Incremental area under curve (iAUC) for metabolic health | Changes in incremental area under curve (iAUC) for insulin and glucose between trials | 320 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Gut hormones responses | Changes in blood gut hormones responses (e.g., GLP-1, PYY and GIP, etc.) between trials | 320 minutes | 
| Visual analogue scale (VAS) | Changes in VAS from 0-100 mm (e.g., appetite and mood, etc.) between trials | 320 minutes | 
| Energy intake | Changes in energy intake (e.g, using a food diary to record) after leaving the laboratory until midnight | 600 minutes after leaving the labalotory | 
| Physical activity level | Changes in physical activity level (e.g., using an accelerometer to record) after leaving the laboratory until midnight | 600 minutes after leaving the labalotory | 
| Lipid profile | Changes in blood lipid profile (e.g., TAG and cholesterol, etc.) between trials | 320 minutes | 
| Blood pressure | Changes in blood pressure (e.g., diastolic and systolic blood pressure) between trials | 320 minutes | 
| Computerized cognitive testing | Changes in cognitive function (e.g., accuracy and reaction time) between trials | 320 minutes | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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GLP-1, PYY, Breaking sitting, Energy balance, Postprandial glycaemia