Insulinotropic Effect of GIP and GLP-1 Before and After Reduced Glucose Tolerance

Study Overview
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Brief Summary
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The incretin effect in patients with type two diabetes is reduced. The investigators have previously shown that it is possible to induce a defect in the incretin effect in healthy individuals. The purpose of this study is to evaluate the insulinotropic affect of the incretin hormones in healthy individuals before and after a deterioration of the glucose homeostasis.

Detailed Description
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The incretin effect in patients with type two diabetes is reduced. The investigators have previously shown that it is possible to induce a defect in the incretin effect in healthy individuals. The purpose of this study is to evaluate the insulinotropic affect of the incretin hormones in healthy individuals before and after a deterioration of the glucose homeostasis. The evaluation is done by infusing GIP, GLP-1 or saline during hyperglycemic clamps.

Official Title
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Insulinotropic Effect of GIP and GLP-1 Before and After Reduced Glucose Induced by Steroid Treatment, Relative Physical Inactivity and High Calorie Diet

Conditions
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Insulin Resistance, Deteriorated Glucose Homeostasis, Inactivity

Intervention / Treatment
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* Behavioral: Steroid hormone
* Other: No intervention


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Caucasians without type 2 diabetes mellitus Normal OGTT (75 g of glucose) according to WHO's criteria BMI 20-30 Hemoglobin > 8.0 mmol/l Informed consent Exclusion Criteria: Liver disease (ALAT > 2 x normal level) Nephropathy (s-creatinin > 130 µM or albuminuria) Relatives (parents/siblings) with T2DM Medical treatment witch cannot be stopped for 12 hours

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Single Group Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: No intervention<br>Each participant is examined during a normal, active lifestyle, without any intervention: The examinations include an OGTT, a glucagon test, three hyperglycemic clamps with infusion of a)GLP-1; b)GIP; c)Nacl | Other: No intervention<br> <br> |
| Experimental: Intervention<br>Each participant is examined during a 12 days intervention.The examinations include an OGTT, a glucagon test, three hyperglycemic clamps with infusion of a)GLP-1; b)GIP; c)Nacl | Behavioral: Steroid hormone<br>* Steroid hormone: 37,5 mg of prednisolone; High energy diet: 130 % of recommended daily energy intake; Relative physical inactivity: no exercise and at least 8 hours of rest/day<br>* Other names: Prednisolone;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Insulinotropic effect of incretin hormones | Measurements of the insulinotropic effect of incretin hormones before and after 12 days intervention with steroid treatment, high calorie diet and relative physical inactivity | 12 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma concentration of glucagon | Measurements of plasma concentration of glucagon before and after 12 days intervention with steroid treatment, high calorie diet and relative physical inactivity | 12 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Incretin hormones, Glucagon, Insulin/c-peptide, Plasma glucose, Insulin resistance, Deteriorated glucose homeostasis, Inactivity