Analysis of the Impact of Cholecystectomy on Gut Microbiome in Adults

Study Overview
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Brief Summary
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Laparoscopic cholecystectomy (LC) is widely used in the gallbladder stone, gallbladder polyp, gallbladder cancer and other diseases. Some of the patients would develop diarrhea after surgery, and studies have reported an increased risk of colorectal cancer after LC. The aim of the present study was to examine the effects of LC on the gut microbiota.

Official Title
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Analysis by High-throughput Sequencing: the Impact of Cholecystectomy on Gut Microbiome in Adults

Conditions
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Laparoscopic Cholecystectomy

Intervention / Treatment
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* Procedure: Laparoscopic cholecystectomy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Gallbladder polyps diameter mm >10; The history of the symptoms of patients with gallbladder stones, there was no history of acute attack in the past month; Age <70 years old; No serious systemic diseases such as diabetes, hypertension, heart disease, lung disease and so on/American anesthesia Association (ASA) grade I or II; BMI<30; Families have better care and observation, and away from the hospital within 1 hours; patients and their families understand the process of ambulatory surgery (24 hours) and the pros and cons. Exclusion Criteria: Less than 18 years old; With previous gastric surgery ; Major systemic diseases ; Pregnancy or lactation; The use of antibiotics or yogurt within 2 months before the study.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Laparoscopic cholecystectomy<br>A laparoscopic cholecystectomy was performed to remove the gallbladder | Procedure: Laparoscopic cholecystectomy<br>* A special catheter was inserted into the peritoneal cavity, and then about 2-5 liters of carbon dioxide was injected. After reaching a certain pressure, four small holes of 0.5-1.5cm were opened in the abdomen to dissect the structure of the triangle of the gallbladder. The gallbladder duct and gallbladder artery were cut off and closed, and then the entire gallbladder was removed.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change of gut microbiota after laparoscopic cholecystectomy | Three months and one year after completion of laparoscopic cholecystectomy therapy, gut microbiota was assessed by 16S rDNA high-throughput sequencing. | Three months and one year after completion of LC | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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laparoscopic cholecystectomy, gut microbiota