Metabolic Response to Fat and Glucose

Study Overview
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Brief Summary
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The study will compare plasma and urine post-prandial metabolomics after fat and glucose oral load according to lifestyle factors.

Detailed Description
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The metabolic response in plasma and urine, pre- and post-load tests in people with specific dietary lifestyles will be tested. This project will focus on fat and glucose induced changes of targeted metabolic pathways that have been related to diabetes and obesity risk.

Official Title
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Metabolic Response to Fat and Glucose

Conditions
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Obesity, Insulin Resistance, Healthy

Intervention / Treatment
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* Other: Glucose tolerance test
* Other: Fat tolerance test


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy volunteers On a stable dietary habits Exclusion Criteria: Pregnancy Malabsorption disorders Present or former cancer Any oral medication that affect glucose or fat absorptions Cholesterol lowering drugs (i.e. Statins) other drugs: fibrates, MTX, or vitamin supplements Diabetes (type 1 and type 2) Alcoholism Weight losing diet during the last 4 months Liver diseases Renal dysfunction Gastric resection.

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Glucose tolerance test<br>All participants will receive an oral standardized glucose tolerance test | Other: Glucose tolerance test<br>* Standardized oral glucose tolerance test (75 g oral glucose) and blood collection before and 2hrs after the glucose load<br>|
| Other: Fat tolerance test<br>The same participants will receive an oral standardized fat load test | Other: Fat tolerance test<br>* Oral fat tolerance will be provided to fasting participants and blood samples will be collected before and 4 hrs after the fat load.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma levels of branched chain amino acid |  | Changes between baseline and 2 hours | 
| Plasma Trimethylamin N-oxide |  | Changes between baseline and 2 hours | 
| Plasma glucose level |  | Changes between baseline and 2 hours | 
| Plasma glucose |  | Changes between baseline and 2 hours | 
| Plasma level of acetyl-CoA |  | Changes between baseline and 2 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma malonyl-CoA |  | Changes between baseline and 2 hours | 
| Plasma phosphatidylcholine |  | Changes between baseline and 2 hours | 
| Plasma insulin |  | Changes between baseline and 2 hours | 
| Plasma leptin |  | Changes between baseline and 2 hours | 
| Plasma Lactate |  | Changes between baseline and 2 hours | 
| Plasma pyruvate |  | Changes between baseline and 2 hours |