Effects of Hydrolysed Porcine Proteins on Muscle Protein Synthesis and Appetite

Study Overview
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Brief Summary
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The purpose of the study is to obtain a better understanding of how hydrolysed porcine proteins affect the human metabolism, including the effects on muscle protein synthesis, appetite and secretion of gastrointestinal hormones. It is hypothesised that hydrolysed porcine proteins will stimulate muscle protein synthesis, affect appetite and the secretion of gastrointestinal hormones similar to hydrolysed whey protein.

Detailed Description
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Previous data show that proteins enhance satiety, decrease hunger and decrease energy intake. In addition, amino acids from dietary protein act as building blocks for de novo protein synthesis and consumption of dietary protein therefore stimulates protein synthesis. Proteins from different sources differ in amino acid composition and rate of absorption. Previously, studies on the effects of proteins on especially muscle protein synthesis has focused on milk proteins, whey and casein as animal sources, but other protein sources high in essential and branched-chain amino acids may also have beneficial effects on muscle protein synthesis and appetite. Thus, in this study the effects of two hydrolysed porcine proteins (one from porcine blood and one from porcine muscle) on muscle protein synthesis, appetite and secretion of gastrointestinal hormones will be compared with the effects of hydrolysed whey protein. These porcine proteins have never previously been tested and it is hypothesised that they may induce similar effects as hydrolysed whey protein due to the high contents of essential and branched-chain amino acids. The study will be conducted as a randomised, 3-way, cross-over study. It consists of three visits separated by at least two weeks. It is expected that 18 normal-weight, young men will complete the study. They will be randomised to the order of the three proteins; hydrolysed porcine protein from blood (HPB), hydrolysed porcine protein from muscle (HPM) and hydrolysed whey protein (HW). At each visit the effects on muscle protein synthesis, appetite and secretion of gastrointestinal hormones will be measured/assessed. Muscle protein synthesis will be measured after consumption of a low (15 g) and a high (30 g) dose of protein and appetite and secretion of gastrointestinal hormones will be measured after the high protein dose (30 g). The effect on muscle protein synthesis will be measured by a flood-primed continuous infusion of labelled (Ring13C6) phenylalanine, muscle biopsies and blood samples. The effects on appetite will be assessed by visual analogue scales, an ad libitum meal and blood samples.

Official Title
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Effects of Hydrolysed Porcine Proteins on Muscle Protein Synthesis and Appetite

Conditions
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Hunger, Satiety Response, Protein Synthesis

Intervention / Treatment
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* Dietary Supplement: Hydrolysed porcine protein from blood
* Dietary Supplement: Hydrolysed porcine protein from muscle
* Dietary Supplement: Hydrolysed whey protein


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy young men Normal weight (BMI 18.5-25 kg/m2) 22-40 years Exclusion Criteria: Vegetarians Weight change >3kg within 2 months prior to start of the study Regular participation in cardio/strength training within 6 months prior to (and during) the study (>1 per wk) Use of alcohol >14 drinks/wk Drug abuse Smoking Regular consumption of protein supplements Use of over-the-counter or prescription medication that influences body weight, appetite or metabolism Diabetes Mellitus Dyslipidemia Diseases, which influence metabolism Donation of blood 3 months prior to start of (and during) the study Subjects with a hemoglobin value < 8 mol/L (measured at screening) Participation in other clinical studies 1 month prior to start of (and during) the study Subjects who are unable to give an informed consent.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Hydrolysed porcine protein from blood<br>Dietary intervention with hydrolysed porcine protein from blood | Dietary Supplement: Hydrolysed porcine protein from blood<br>* Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite<br>|
| Experimental: Hydrolysed porcine protein from muscle<br>Dietary intervention with hydrolysed porcine protein from muscle | Dietary Supplement: Hydrolysed porcine protein from muscle<br>* Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite<br>|
| Experimental: Hydrolysed whey protein<br>Dietary intervention with hydrolysed whey protein | Dietary Supplement: Hydrolysed whey protein<br>* Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in Muscle protein synthesis (%FSR) |  | 3 biopsies are taken at time 0, 150 min and 310 min at each visit | 
| Change in Muscle protein synthesis (%FSR) |  | 13 blood samples are taken over 310 min | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Visual analogue scales (10 cm) | Subjective appetite | Assessed at time 0, 160, 175, 200, 220, 250, 280, 310, 345 min at each of the three test days | 
| Appetite - ad libitum energy intake | At 325 min an ad libitum meal of spaghetti bolognese is served, and the total energy intake is recorded | Measured once at time 325 min at each of the three test days | 
| Postprandial response in glycemic control, plasma amino acids and appetite regulating hormones |  | Blood samples are taken at 0, 150, 180, 200, 220, 250, 280 and 310 min at each of the three test days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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porcine protein, appetite, muscle protein synthesis, gastrointestinal hormones