Study of Anti-5T4 CAR-raNK Cell Therapy in Locally Advanced or Metastatic Solid Tumors

Study Overview
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Brief Summary
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This study is a multicenter, open-label, investigator-initiated trial (IIT), divided into dose escalation (Part A) and dose extension (Part B) phases to evaluate the safety, tolerability, pharmacokinetics (PK), pharmacokinetics (PD) and initial efficacy of conjugated antibody redirecting ready-to-use allogeneic NK (CAR-raNK) cells that target trophoblast glycoprotein (5T4) in patients with locally advanced or metastatic solid tumors.

Detailed Description
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Part A is a dose escalation study to evaluate maximum toxic dose (MTD) and/or recommended phase II dose (RP2D) which adopts the 3+3 dose escalation design protocol. The dose is respectively 3.0×10^9 live cells, 6.0×10^9 live cells and 9.0×10^9 live cells (if the safety of dose group with 9.0×10^9 live cells is still good, the Safety Monitoring Committee (SMC) will co-decide whether to continue the dose escalation and the specific dose based on the obtained data on safety, efficacy, and PK). The 3.0×10^9 live cell dose group is given on days 1 and 3 of each cycle (21 days), and the follow-up dose group is given on days 1 and 8 of each cycle (21 days).3-6 subjects will be enrolled at every dose level. The first and second subjects in the same group shall be enrolled at an interval of at least 7 days, for the purpose of ensuring their safety. Only when the dose-limiting toxicity (DLT) of all subjects in the previous dose group was observed can the enrollment of the next dose group get started. Part B is the dose extension study. After recommended phase II dose (RP2D) is determined in Part A, the SMC will discuss whether to conduct the Part B study. This stage will be carried out in different tumor types with high expression of 5T4 antigen, and 6 to 10 subjects will be enrolled in each tumor type, and all subjects will receive anti-5T4 CAR-raNK cell therapy at RP2D level. Every 21 days is one cycle, and the administration is performed on day 1 and day 8 of each cycle.

Official Title
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Dose Escalation and Extension Study to Evaluate the Safety, Tolerability, and Initial Efficacy of Anti-5T4 CAR-raNK Cell Therapy in Locally Advanced or Metastatic Solid Tumors

Conditions
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Locally Advanced or Metastatic Solid Tumors

Intervention / Treatment
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* Biological: Anti-5T4 CAR-raNK Cells


Participation Criteria
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Eligibility Criteria
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Inclusion criteria: Subjects volunteer to participate in this clinical study, are fully aware of the study and have signed the Informed Consent Form (ICF). Subjects are willing to follow and able to complete all trial procedures. Age: adult at the age of 18-80 (both inclusive), female or male. Patients with advanced malignant solid tumors, histologically or cytologically confirmed, who had failed standard therapy, or had no standard therapy, or were not eligible for standard therapy at this stage; Part B: Patients with specific primary tumor types, including: Cohort 1: Non-small cell lung cancer with disease progression or intolerance after at least two systemic therapies; For patients with known epidermal growth factor receptor (EGFR)-sensitive mutations, anaplastic lymphoma kinase (ALK) gene fusion, or other driver gene positivity, progression must be followed by appropriate targeted therapy. Cohort 2: Breast cancer, negative for progesterone receptor (PR), estrogen receptor (ER), and human epidermal growth factor receptor-2 (HER2), treated with at least one systemic chemotherapy agent. Cohort 3: Colorectal cancer with disease progression or intolerance after at least two systemic therapies. Cohort 4: Mesothelioma with disease progression or intolerance after at least two systemic therapies. Cohort 5: Other tumors with high expression of antigen 5T4. Eastern Cooperative Oncology Group (ECOG) score ≤1 and expected survival time > 3 months. (Part A) According to RECIST v1.1, there is at least one assessable tumor lesion; (Part B) According to RECIST v1.1, there is at least one measurable tumor lesion (a lesion within the field of previous radiation cannot be targeted unless there is radiographic evidence of progression or persistence after 3 months of radiation). Organ function during screening should meet the following criteria: Hematologic system (no blood transfusion or hematopoietic stimulator treatment within 14 days) Absolute neutrophil count (ANC) ≥1.5×109/L Platelet (PLT) ≥75×109/L Hemoglobin (Hb) ≥85g/L Hepatic function Total bilirubin (TBIL) ≤1.5×ULN Alanine aminotransferase (ALT) ≤3×ULN; Patients with liver metastasis or liver cancer: ≤5×ULN Aspartate aminotransferase (AST) ≤3×ULN; Patients with liver metastasis or liver cancer: ≤5×ULN Renal function Creatinine (Cr) ≤1.5× ULN Creatinine clearance (Ccr) (to be calculated only when Cr > 1.5× ULN) > -50ml/min/1.73m2 (Cockcroft-Gault formula) Coagulation function Activated partial thrombin time (APTT) ≤1.5×ULN International normalized ratio (INR) ≤1.5×ULN Subjects of reproductive age and their partners should agree to have no family planning and to use effective contraceptive methods (hormonal or barrier methods or abstinence, etc.) for 6 months from signing the ICF until the last dose of the study drug is administered; women of reproductive age must have a negative serological pregnancy test 7 days prior to their first use of the study drug. Exclusion Criteria: Have received systemic antitumor therapy, including chemotherapy, immunotherapy, and radical radiotherapy, within 4 weeks prior to their first use of the study drug. The following special cases should be assessed separately: The time of the last treatment of nitrosourea or mitomycin C is less than 6 weeks before the first use of the study drug; The time of last treatment of fluorouracil and small-molecule targeted drugs is less than 2 weeks or 5 half-lives of the drug (whichever was longer) after the first use of the study drug; The time of the last treatment of the traditional Chinese medicine with anti-tumor indications was less than 2 weeks after the first use of the study drug. Have participated in other clinical trials and received any unmarketedinvestigational drug or treatment within 4 weeks prior to first use of the study drug. Any prior adoptive cellular immunotherapy. Have undergone major organ surgery (excluding needle biopsy) or had significant trauma within 4 weeks prior to their first use of the study drug, or required elective surgery during the study period. Have received systemic glucocorticoids (prednisone> 10 mg/ day or an equivalent dose of another drug of the same class) or other immunosuppressants within 14 days prior to initial use of the study drug. The exceptions are: local, ocular, intraarticular, intranasal, and inhaled glucocorticoids; short-term use of glucocorticoids for prophylaxis (e.g., to prevent contrast allergy). Have received live, attenuated, adenovirus, or messenger ribonucleic acid (mRNA) vaccines within 4 weeks prior to initial use of the study drug, or plan to receive these vaccines during the study period. Have used immunomodulatory drugs, including but not limited to thymosin, interleukin-2, interferon, etc., within 14 days prior to their first use of study drugs. Patients with active infection who currently require intravenous anti-infective therapy. Active hepatitis b (HBsAg positive and hepatitis b virus (HBV) DNA Patients with a known history of human immunodeficiency virus (HIV) infection, or other acquired, congenital immunodeficiency disease, or a history of organ transplantation. Have active autoimmune diseases or have had autoimmune diseases that are likely to recur (e.g., systemic lupus erythematosus, rheumatoid arthritis, inflammatory bowel disease, autoimmune thyroid disease, vasculitis, psoriasis, etc.). Except in the following cases: type 1 diabetes that was well controlled with hormone replacement therapy, hypothyroidism, skin conditions that did not require systemic therapy (e.g., vitiligo), and other conditions that were well controlled and that the investigator determined were less likely to recur (e.g., childhood asthma in remission). Have a history of serious cardiovascular and cerebrovascular diseases, including but not limited to: There are serious cardiac rhythm or conduction abnormalities, such as ventricular arrhythmia, and Ⅱ-Ⅲ degree atrioventricular block, which need clinical intervention; The mean QT interval (QTcF) corrected by Fridericia method was > 480ms; Acute coronary syndrome, congestive heart failure, aortic dissection, stroke, or other grade 3 or above cardiovascular and cerebrovascular events occurring within 6 months before the first administration; Patients with heart failure or left ventricular ejection fraction (LVEF) < 50% in the New York Heart Association (NYHA) classification ≥II; Hypertension beyond clinical control. Previous or current interstitial lung disease (except local interstitial pneumonia induced by radiotherapy). Adverse effects of previous antineoplastic therapy have not returned to CTCAE grade 5.0 ≤1 (except for toxicity that the investigator determined to be of no safety risk, such as alopecia, grade 2 peripheral neurotoxicity, and hypothyroidism stabilized by hormone replacement therapy). Cerebral parenchymal metastasis or meningeal metastasis with clinical symptoms were deemed unsuitable for inclusion by the investigator. Had received immunotherapy and developed grade ≥3 immune-related adverse events (irAE). The third interstitial effusion, which could not be controlled clinically, was judged by the investigator to be unsuitable for inclusion. (Extension phase) had other malignant tumors in the past 5 years, excluding skin basal cell carcinoma, ductal carcinoma in situ and cervical carcinoma in situ with a radical surgery. Pregnant or lactating women. Have a history of alcohol or drug abuse. Mental disorder or poor compliance. The investigator considered that the subjects had a history of other serious systemic diseases or other reasons that made them unsuitable for the study.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Sequential Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Anti-5T4 CAR-raNK Cells<br> | Biological: Anti-5T4 CAR-raNK Cells<br>* In the 3+3 dose escalation study, the minimum initial dose is 3.0×10^9 cells and then escalate to 6.0× 10^9 and 9.0× 10^9 cells. Every 21 days is one cycle, and intravenous infusion is performed on day 1 and day 3\8 of each cycle. In dose extension study, the initial dose will be determined by RP2D determined by the results of dose escalation study, and the other intervention methods are consistent.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Part A: Incidence of dose limiting toxicity (DLTs) | To evaluate the safety, tolerability, and determine the RP2D of Anti-5T4 CAR-raNK Cells | From day1 to day 21 | 
| Part A: Number of Adverse Events (AEs) | To evaluate the safety of Anti-5T4 CAR-raNK Cells | From day 1 to day 90 after the last dose | 
| Part B: Objective response rate (ORR) | To determine the anti-tumor effectivity of Anti-5T4 CAR-raNK Cells | Up to 1 year after infusion | 
| Part B: Disease control rate (DCR) | To determine the anti-tumor effectivity of Anti-5T4 CAR-raNK Cells | Up to 1 year after infusion | 
| Part B: Duration of remission (DOR) | To determine the anti-tumor effectivity of Anti-5T4 CAR-raNK Cells | Up to 1 year after infusion | 
| Part B: Progression-free survival (PFS) | To determine the anti-tumor effectivity of Anti-5T4 CAR-raNK Cells | Up to 1 year after infusion | 
| Part B: Overall survival (OS) | To determine the anti-tumor effectivity of Anti-5T4 CAR-raNK Cells | Up to 1 year after infusion | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The number of CAR-raNK cells | Blood samples will be collected at specified time points to detect the number of CAR raNK cells in peripheral blood | From day1 to day 21 | 
| Cytokine release | Blood samples will be collected at specified time points to detect serum Blood samples will be collected at specified time points to detect the cytokine (IL-1β, IL-2, IL-4, IL-6, IL-10, IFN-γ, TNF-α) concentration | From day1 to day 21 | 
| Lymphocyte subtype | Blood samples will be collected at specified time points to analyze the lymphocyte subtypes (CD3, CD4, CD8, CD19, CD56). | From day1 to day 21 | 
| Anti-CAR antibodies | Blood samples will be collected at specified time points to detect anti-CAR(anti-5T4 mAb) antibodies | From day1 to day 21 |