Recombinant Human rhPTH(1-34) VS Association Alfacalcidol/Hydrochlorothiazide in Severe Primary Hypoparathyroidism

Study Overview
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Brief Summary
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Hypoparathyroidism is a rare condition in which the parathyroid glands fail to produce sufficient amount of parathyroid hormone or the parathyroid hormone produced lacks biologic activity. The most common cause of hypoparathyroidism is damage to or removal of the parathyroid glands due to neck surgery for another condition. Occurrence of hypercalciuria under treatment is a frequent concern in primary hypoparathyroidism, limiting correction of hypocalcemia. Hypoparathyroidism can also be caused by an autoimmune process. In rare cases, hypoparathyroidism may occur as a genetic disorder inherited as an autosomal recessive, autosomal dominant or X-linked recessive trait. The autosomal dominant hypocalcemia (ADH) is mainly caused by heterozygous activating mutations in the CASR gene encoding CaSR). As other severe presentation of primary hypothyroidism, ADH is characterized by the increased risk to develop hypercalciuria and nephrolithiasis. The purpose of the study is to compare two therapeutic approaches in severe hypoparathyroidism in order to limit the risk of nephrocalcinosis and renal failure when attempting to correct hypocalcemia: rhPTH(1-34) vs association of active vitamin D and hydrochlorothiazide. The European Society of Endocrinology Clinical has indeed recently published guidelines for the treatment of chronic hypoparathyroidism in adults. These guidelines suggest considering treatment with a thiazide diuretic In a patient with hypercalciuria and replacement therapy with PTH in patients who do not stably and safely maintain their serum and urinary calcium in the target range.

Detailed Description
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The design consists in a five-periods, two-treatments, open-label, randomized, crossover study with blind end-point evaluation. Patients will come for an inclusion visit and will receive treatment with 0.5 µg/day alfacalcidol for 4 weeks (28±3 days, run-in). They will be instructed to maintain dietary calcium intakes (1 g/day) for the duration of the study and will be supplemented throughout the study with native vitamin D in order to maintain the concentration of 25OH vitamin D ≥ 40 ng/L. Magnesium supplementation (100 mg/day) will be maintained throughout the study. At inclusion, patients will be randomly assigned to receive at the end of run-in period, in cross-over either an association hydrochlorothiazide 25 mg/day (ESIDREX®) + amiloride 5 mg/day (MODAMIDE®) + 0.5 µg/day alfacalcidol (ALFACALCIDOL®) or 40 µg/day rhPTH(1-34) (teriparatide or FORSTEO® 20 µg twice daily) over 7 to 8 weeks (52±3 days). After a washout period of 28±3 days under 0.5 µg alfacalcidol /day, the patients will follow the second period of treatment. The study will end with a final period of 28±3 days under 0.5 µg alfacalcidol /day. Patients will ambulatory monitor serum calcium, sodium, potassium, and creatinine levels at days 15 of run in and run out periods and at day 7 and day 28 of each treatment period.

Official Title
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A Randomized Crossover TrIal to Compare Recombinant Human rhPTH(1-34) to the ASsociation Alfacalcidol/Hydrochlorothiazide in the Treatment of Severe Primary Hypoparathyroidism

Conditions
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Autosomal Dominant Hypocalcemia OR Primary Hypoparathyroidism Related to Other Cause But Complicated by Hypercalciuria Under Treatment

Intervention / Treatment
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* Drug: Teriparatide
* Drug: Thiazide
* Drug: Potassium sparing diuretic
* Drug: Alfacalcidol


Participation Criteria
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Eligibility Criteria
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Inclusion criteria : Patients aged from 18 to 80 years, of both sexes Patient with primary hypoparathyroidism related to a genetically proven ADH OR primary hypoparathyroidism related to other cause but complicated by hypercalciuria under treatment Affiliated to a French health insurance system, and who have consented to the study. Exclusion criteria : Pregnant and breastfeeding women; Women of childbearing age without contraception; For men aged from 18 to 20 years, presence of cartilage of growth on X-ray of left knee; Anuria; Kidney failure with plasmatic creatinine >125 mmol/l and urea >10 mmol/l; Long QT interval : QTc > 450 ms (men) or 470 ms (women); Hepatic failure; Metabolic bone diseases (Paget's disease of bone) other than primary osteoporosis or glucocorticoid-induced osteoporosis; Association to other potassium sparing diuretics; Hypokalemia (<3.5 mmol/l) without diuretic therapy; Hyperkalemia (>5.5 mmol/l); Hyponatremia (<135 mmol/l) without diuretic therapy; Hypercalcemia (>2.6 mmol/l); Severe hypomagnesemia (≤ 0.5 mmol/l); Vitamin D deficiency (25OH vit D < 20 ng/mL); Unexplained increase in alkaline phosphatase (>2N); Intolerance to sulfamide; Intolerance to amiloride or other component of the drug; Hypersensitivity to any active substance or excipient of one of the experimental drugs; Gluten intolerance; Bone break history within the three previous months; History of radiotherapy of the skeleton; History of bone cancer or metastasis. Personnal or familial (first degree relatives) of skin cancer

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: rh PTH(1-34)<br>40 µg/day rhPTH(1-34) (teriparatide or FORSTEO® 20 µg twice daily) over 7 to 8 weeks (52±3 days). | Drug: Teriparatide<br>* human recombinant parathormone<br>* Other names: FORSTEO;|
| Active Comparator: Thiazide + potassium sparing diuretic<br>hydrochlorothiazide 25 mg/day (ESIDREX®) + amiloride 5 mg/day (MODAMIDE®) + 0.5 µg/day alfacalcidol (ALFACALCIDOL®) over 7 to 8 weeks (52±3 days). | Drug: Thiazide<br>* Diuretic<br>* Other names: ESIDREX;Drug: Potassium sparing diuretic<br>* Diuretic<br>* Other names: MODAMIDE;Drug: Alfacalcidol<br>* Belongs to the class of vitamin D and analogues<br>* Other names: UN-ALFA;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma calcium concentration | Mean of two measures at 30-min interval of Ionized serum calcium concentration | two months of treatment | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Ambulatory calcium concentration | Ambulatory measurement of serum calcium level | days 7 an 28 of treatment by rhPTH(1-34) and association alfacalcidol/hydrochlorothiazide and at day 14 of non-treatment periods (run in, wash out, run out). | 
| Calciuria | 24h-urinary calcium excretion (expressed as mmol/24h and mmol/mmol creatinine) | Inclusion, weeks 4 (end of the run-in period), 7-8 (end of the first treatment period), 11-12 (end of the wash-out period), 18-20 (end of the second treatment period), 202 (end of the wash-out period) | 
| Plasma calcium x phosphate product |  | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Blood pressure | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Serum sodium level | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Serum potassium level | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Estimated GFR using MDRD formula | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Serum renin level | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Serum aldosterone level | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| 24h-urinary sodium excretion | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| 24h-urinary potassium excretion | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| 24h-urinary aldosterone excretion | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Serum 25 OH vitamin D level | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Serum 1,25(OH)2 vitamin D level | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Serum magnesium level | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| 24h-urinary magnesium excretion | Tolerance of thiazides and amiloride | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Calcium/citrate ratio measured on spot urines | Assessment of stone formation risk | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Calcium/creatinine ratios measured on spot urines | Assessment of stone formation risk | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Crystalluria | Assessment of stone formation risk | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Alkaline phosphatase level | Evaluation of the impact of rhPTH(1-34) on bone | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Number of episodes of cramps | Other tolerance | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Number of episodes of paresthesia | Other tolerance | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Number of episodes of tetany | Other tolerance | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| Number of episodes of seizure | Other tolerance | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 
| SF36 self-administered questionnaire | Evaluation of the impact on quality of life | Inclusion, days 28 (end of the run-in period), 80 (end of the first treatment period), 108 (end of the wash-out period), 160 (end of the second treatment period), 202 (end of the wash-out period) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Hypoparathyroidism, PTH(1-34), Thiazides, Hypercalciuria, Calcium sensing receptor