Value of Von Willebrand Factor and Copeptin Assay in Children With Febrile Seizures at Sohag University Hospital

Study Overview
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Brief Summary
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Febrile seizures are one of the most common clinical diseases in pediatric neurology. It occurs between 6 months and 6 years of age and occurs in  2-5% of children. According to the age, frequency, duration, and type of seizures FS is divided into simple febrile seizures and complex febrile seizures Differentiation between febrile seizures and non-ictal events associated with fever such as shivering or dizziness is challenging. Therefore, precise diagnosis of FS after paroxysmal episodes associated with fever is often hindered by the lack of an objective biomarker With the widespread application of technologies, such as molecular biology, in medicine, some biomarkers for predicting or diagnosing FS have attracted attention. Imuekemhe et al in 1989 and 1996 found that lactic acid in the serum and cerebrospinal fluid of children with FS was significantly increased . Arginin-vasopressin hormone AVP released by the pituitary gland, has been shown to be involved in the thermoregulatory response to fever and convulsions Although AVP is unstable in the peripheral blood and, therefore, unsuited for diagnostic use the C-terminal portion of the AVP precursor copeptin has been recognized as a robust marker of AVP secretion . Wellman et al. found that the serum copeptin and Von Willebrand factor of children with FS were significantly higher than those of the control group .

Official Title
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Value of Von Willebrand Factor and Copeptin Assay in Children With Febrile Seizures at Sohag University Hospital

Conditions
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Febrile Seizure

Intervention / Treatment
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* Diagnostic Test: von willebrand factor and copeptin


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age from 6 months to 6 years. Seizures. Fever (≥38°C). Exclusion Criteria: Central nervous system infection. Epilepsy. Previous neurological abnormalities. Inborn errors of metabolism. Immunological diseases. Endocrinal diseases (e.g., diabetes mellitus). Obesity. Eating disorders. Gastrointestinal disorders (e.g., diarrhea).

Ages Eligible for Study
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Minimum Age: 6 Months
Maximum Age: 6 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| children with febrile seizures<br> | Diagnostic Test: von willebrand factor and copeptin<br>* biomarkers increase in children with febrile seizures<br>|
| febrile children without seizures<br> | Diagnostic Test: von willebrand factor and copeptin<br>* biomarkers increase in children with febrile seizures<br>|
| healthy control children<br> | Diagnostic Test: von willebrand factor and copeptin<br>* biomarkers increase in children with febrile seizures<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Value of von willebrand factor and copeptin assay in children with febrile seizures at Sohag University Hospital | Serum copeptin and Von Willebrand factor (VWF) venous blood samples (3 ml) will be obtained from children with FS (within 3 hours of seizures) as well as the two control groups. . | one year |