Anti-inflammatory Cells in Peritoneal Cavity

Study Overview
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Brief Summary
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Some subsets of lymphocytes are able to inhibit immune response and thus, could be used to control auto-immune diseases and transplant reject. In mice, the main source of those anti-inflammatory lymphocytes is the peritoneal cavity. No data are available in human. This study aims at exploring the presence of those anti-inflammatory lymphocytes in human peritoneal cavity and at determine how to expand those cells.

Detailed Description
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B cells will be collected during procedures planned for patients' standard care. Peritoneal fluid and peripheral blood of patients will be collected during coelioscopy for exploration of ovarian cysts or dialysis for treatment of chronic renal insufficiency. Regulatory B cells (Breg cells) will be measured in peritoneal fluid and in peripheral blood after in vitro stimulation and assessment of intracellular interleukin-10 by flow cytometry. Proportion of Breg cells among B cells will be compared between peritoneal fluid and peripheral blood in both groups. Different protocols to induce differentiation of B cells into Breg cells will be tested and compared.

Official Title
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Can the Peritoneal Cavity be a Source of Regulatory B Cells to Treat Auto-immune Disease?

Conditions
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Ovarian Cysts, Chronic Renal Insufficiency

Intervention / Treatment
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* Other: peritoneal fluid and blood sample


Participation Criteria
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Eligibility Criteria
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Inclusion criteria: woman over 18 year old undergoing exploration of ovarian cysts by coelioscopy patient with chronic renal insufficiency undergoing peritoneal dialysis informed consent obtained Exclusion criteria: pregnancy or breast-feeding non affiliation to health care insurance treatment with corticosteroids over 15 mg/day treatment with rituximab

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: subjects with ovarian cyst<br>Peritoneal fluid sample obtained from coelioscopy for exploration of ovarian cysts and blood sample | Other: peritoneal fluid and blood sample<br>* Peritoneal fluid sample obtained from peritoneal dialysis fluid of patients with chronic renal insufficiency and blood sample or Peritoneal fluid obtained from coelioscopy for exploration of ovarian cysts and blood cell<br>|
| Other: subjects with peritoneal dialysis<br>Peritoneal fluid sample obtained from peritoneal dialysis fluid of patients with chronic renal insufficiency and blood sample | Other: peritoneal fluid and blood sample<br>* Peritoneal fluid sample obtained from peritoneal dialysis fluid of patients with chronic renal insufficiency and blood sample or Peritoneal fluid obtained from coelioscopy for exploration of ovarian cysts and blood cell<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| relative percentage of regulatory B cells among B cells in peritoneal cavity compared to peripheral blood | Comparison of Breg cell in peritoneal cavity to Breg cell in peripheral blood B | up to 48 hour | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Peritoneal fluid, B cells, Regulatory B cells, Human