Study of the Effect of Oral Zinc Supplementation on Superoxide Radical Scavengers

Study Overview
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Brief Summary
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Although several studies have considered the relationship between infertility and semen super oxide radical scavengers levels, no study on the effects of asthenospermia treatments such as oral zinc supplementation on superoxide radical scavengers activity which are important in fertility of the individual has been reported.

Detailed Description
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The present study was conducted to study the effect of zinc supplementation on the quantitative and qualitative characteristics of semen along with superoxide radical scavengers activity in the seminal plasma of asthenospermic patients.

Official Title
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Study of the Effect of Oral Zinc Supplementation on Superoxide Radical Scavengers in Spermatozoa of Patients With Asthenospermia

Conditions
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Asthenozoospermia

Intervention / Treatment
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* Dietary Supplement: zinc sulfate


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: the presence of asthenozoospermia in the semen sample. Exclusion Criteria: the absence of endocrinopathy, varicocele, and female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels.

Ages Eligible for Study
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Minimum Age: 26 Years
Maximum Age: 36 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Zinc sulfate<br>60 subfertile (age 32.5±3.23 year) men with asthenozoospermia was treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg). | Dietary Supplement: zinc sulfate<br>* The subfertile group treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg).<br>|
| No Intervention: Healthy control<br>60 fertile (age 31.6±3.3 year) men, no treatment. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oxido-sensitive index level in spermatozoa and seminal plasma. |  | up to three months. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Volume of semen. |  | up to three months. | 
| Xanthine oxidase activity in spermatozoa and seminal plasma. |  | up to three months. | 
| Catalase like activity in spermatozoa and seminal plasma. |  | up to three months. | 
| Superoxide dismutase activity in spermatozoa and seminal plasma. |  | up to three months. | 
| Progressive sperm motility percentage. |  | up to three months | 
| Total normal sperm count. |  | up to three months. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Zinc supplementation., Oxidative stress., Superoxide radical scavengers., Asthenospermia., Oxido-sensitive index.