Studying DNA in Tumor Tissue Samples From Patients With Localized or Metastatic Osteosarcoma

Study Overview
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Brief Summary
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RATIONALE: Studying samples of tumor tissue and blood from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at DNA in tumor tissue samples from patients with localized or metastatic osteosarcoma.

Detailed Description
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OBJECTIVES: Determine the genomic expression profile in tumor tissue samples from patients with localized or metastatic osteosarcoma using transcriptome sequencing. Identify activating and loss of function mutations and gene rearrangement in these tumor tissue samples using transcriptome sequencing. Identify candidate genes that are important in osteosarcoma and tumorigenesis using genome partition strategies for genomic DNA sequencing. Identify which mutations are associated with outcome. Establish which mutations are also found in germ line DNA that predispose the patient to osteosarcoma. OUTLINE: DNA and RNA from banked tumor tissue samples and DNA from paired blood samples are analyzed in sequencing studies using next-generation sequencing technology. The sequencing data from these tumor samples are matched to the Human RefSeq (for transcriptome sequencing) and normal human genome in the public databases and to the patient's germ line sequence to identify constitutional and somatic mutations. Clinical information that is associated with each sample (i.e., age, tumor site, size, primary metastases, response to chemotherapy, surgical remission, follow-up time, and treatment protocol) is also collected, if available.

Official Title
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Genomic Study of Metastatic Osteosarcoma Using Next-Generation Sequencing Technology

Conditions
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Sarcoma

Intervention / Treatment
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* Genetic: DNA analysis
* Genetic: RNA analysis
* Genetic: gene expression analysis
* Genetic: gene rearrangement analysis
* Genetic: mutation analysis
* Other: laboratory biomarker analysis


Participation Criteria
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Eligibility Criteria
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DISEASE CHARACTERISTICS: Diagnosis of localized or metastatic osteosarcoma Banked snap-frozen tumor tissue samples and paired blood DNA samples available PATIENT CHARACTERISTICS: Not specified PRIOR CONCURRENT THERAPY: Not specified
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Genetic: DNA analysis|nan|
|Genetic: RNA analysis|nan|
|Genetic: gene expression analysis|nan|
|Genetic: gene rearrangement analysis|nan|
|Genetic: mutation analysis|nan|
|Other: laboratory biomarker analysis|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Genomic expression profile in osteosarcoma tumor samples using transcriptome sequencing |  |  | 
| Identification of activating and loss of function mutations and gene rearrangement using transcriptome sequencing |  |  | 
| Mutations associated with outcome |  |  | 
| Mutations also found in germ line DNA that predispose the patient to osteosarcoma |  |  | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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localized osteosarcoma, metastatic osteosarcoma