Fatty Acid Composition of Subcutaneous Adipose Tissue in Infants: A Prospective and Observational Study

Study Overview
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Brief Summary
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The fatty acid composition in blood and subcutaneous adipose tissue of infants that have an clinical indicated operation is studied.

Detailed Description
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Collection of: subcutaneous adipose tissue of term infants and preterms after birth and at term during a clinically indicated operation. blood before the operation. Measurement of the fatty acid composition (especially arachidonic acid and docosahexaenoic acid) in subcutaneous adipose tissue and blood. Aim is to compare the composition of the fatty acids in blood and adipose tissue of preterms at term and term infants. To calculate the fatty acid value in each infant the proportion of adipose tissue is determined with PEAPOD before discharge from hospital.

Official Title
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Untersuchung Intraoperativ Entnommener Subkutaner Fettgewebeproben Früh- Und Reifgeborener Auf Die Fettsäure-Zusammensetzung Der Triglyceride Und Der Phospholipide Mit Speziellem Fokus Auf Die Mehrfach ungesättigten Fettsäuren Docosahexaensäure Und Arachidonsäure Wissenschaftliche Untersuchung Bei Minderjährigen

Conditions
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Prematurity, Subcutaneous Adipose Tissue

Intervention / Treatment
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* Other: adipose tissue
* Other: blood
* Diagnostic Test: PEAPOD


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: meeting criteria of the 3 cohorts Exclusion Criteria: metabolic diseases of the infant genetic diseases missing agreement for the study

Ages Eligible for Study
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Maximum Age: 6 Months

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| preterms with enterostomy<br>GA 23±0/7 - 31±6/7 with clinically indicated operations because of NEC (necrotising enterocolitis) or FIP (focal intestinal perforation). Operation after birth (within 14 days) and at term (enterostomy closure) Analysis of the blood, subcutaneous adipose tissue und total adipose tissue (PEAPOD) | Other: adipose tissue<br>* taking a sample of the adipose tissue during operation<br>Other: blood<br>* taking a blood sample when a peripheral venous catheter is inserted before operation<br>Diagnostic Test: PEAPOD<br>* Measurement of the total adipose tissue of the infant with PEAPOD (Air Displacement-Plethysmography, Infant Body Composition System, Cosmed, Rome, Italy)<br>|
| preterms without enterostomy<br>GA 23±0/7 bis 31±6/7 with a clinically indicated operation (e.g. herniotomy) at term, without any abdominal problems. Analysis of the blood, subcutaneous adipose tissue und total adipose tissue (PEAPOD) | Other: adipose tissue<br>* taking a sample of the adipose tissue during operation<br>Other: blood<br>* taking a blood sample when a peripheral venous catheter is inserted before operation<br>Diagnostic Test: PEAPOD<br>* Measurement of the total adipose tissue of the infant with PEAPOD (Air Displacement-Plethysmography, Infant Body Composition System, Cosmed, Rome, Italy)<br>|
| term infants<br>GA ≥34±0/7 with a clinically indicated operation directly after birth (within 14 days) (e.g. gastroschisis). Analysis of the blood, subcutaneous adipose tissue und total adipose tissue (PEAPOD) | Other: adipose tissue<br>* taking a sample of the adipose tissue during operation<br>Other: blood<br>* taking a blood sample when a peripheral venous catheter is inserted before operation<br>Diagnostic Test: PEAPOD<br>* Measurement of the total adipose tissue of the infant with PEAPOD (Air Displacement-Plethysmography, Infant Body Composition System, Cosmed, Rome, Italy)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Fatty acid composition of the subcutaneous adipose tissue and plasma | Fatty acid composition of the subcutaneous adipose tissue and plasma of preterms at term compared to the composition of infants after birth (within 14 days). | at the time of the operation | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Fatty acid composition of the subcutaneous adipose tissue and plasma of preterms with abdominal problems | Change of fatty acid composition of the subcutaneous tissue and plasma from first to second operation in preterms with abdominal operations, enterostomy | time of the first operation until discharge home (at a postnatal age of up to 4-5 months at the most in very preterm infants) | 
| Fatty acid composition of the subcutaneous adipose tissue and plasma of preterms with abdominal problems and preterms without abdominal problems at term | Fatty acid composition of the subcutaneous adipose tissue and plasma of preterms at term compared to the composition of preterms at term with enterostomy. | time of the first operation until discharge home (at a postnatal age of up to 4-5 months at the most in very preterm infants) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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preterm, fatty acid, docosahexaenoic acid, arachidonic acid, infant, adipose tissue, subcutaneous adipose tissue