Nitrate for High Intensity Intermittent Exercise

Study Overview
=================
Brief Summary
-----------------
The objective of the current study is to assess the effect of 6-day dietary nitrate ingestion on high intensity intermittent sports performance in recreational soccer players.

Detailed Description
-----------------
Oral ingestion of nitrate (NO3-) in the form of both nitrate salts and concentrated red beetroot juice has been shown to significantly lower blood pressure at rest and to improve exercise performance during cycle time trials. Furthermore, a positive exercise performance effect of nitrate ingestion has been observed in recreational athletes during intermittent exercise. However, this was achieved after acute supplementation with a substantial dose of dietary nitrate. Whether similar ergogenic effects of nitrate supplementation can be achieved with a more conventional 6-day nitrate supplementation protocol with a lower daily dose remains to be established. Based on the gaps in current literature, our main goal will be to gain further insight into the effects of a 6-day nitrate supplementation protocol on high intensity intermittent exercise performance in recreational athletes.

Official Title
-----------------
The Effect of Nitrate Supplementation on Sport Performance: High Intensity Intermittent Performance in Recreational Athletes

Conditions
-----------------
High Intensity Intermittent Sports Performance

Intervention / Treatment
-----------------
* Dietary Supplement: Nitrate-rich concentrated beetroot juice
* Dietary Supplement: Nitrate-depleted concentrated red beetroot juice


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Healthy P Players of the first two senior selection teams of a regional soccer club competing at the 3rd KNVB level or higher Training ≥2 days a week 18-40 years Male Exclusion Criteria: Use of medication Injury prohibiting them from performing the exercise protocol effectively Smoking Currently supplementing diet with nitrate Lactose intolerance

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
-----------------
Male

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Nitrate-rich concentrated beetroot juice<br>Dietary supplement: 800 mg of nitrate in 140 mL of concentrated beetroot juice (Beet-IT) | Dietary Supplement: Nitrate-rich concentrated beetroot juice<br>* James White, Beet It Sport Shot, 70ml (Standard)<br>|
| Placebo Comparator: Nitrate-depleted conc. beetroot juice<br>Placebo: 140 mL of nitrate-depleted concentrated red beetroot juice | Dietary Supplement: Nitrate-depleted concentrated red beetroot juice<br>* Beet It Sport Shot, 70ml (Placebo)<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Total distance covered during the Yo-Yo Intermittent Recovery 1 test (Yo-Yo IR1 test) |  | Performance 3 hours post red beetroot juice ingestion | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma concentration of nitrite and nitrate |  | Concetrations 3 hours post red beetroot juice ingestion | 
| Salivary concentration of nitrite and nitrate |  | Concetrations 3 hours post red beetroot juice ingestion | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
Red beetroot juice, High intensity intermittent exercise, Nitrite, Soccer