New Modality for Post Covid Syndrome Patients

Study Overview
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Brief Summary
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Coronavirus (19) is a pandemic that affects many countries allover the world. Many patients experienced symptoms as dyspnea and fatigue after their recovery from coronavirus . These symptoms appear to occur regardless the severity of the infection. In addition, many studies reported change in the inflammatory status and immunity in post covid patients. laser acupuncture is a painless noninvasive modality that is used in treating many diseases. laser acupuncture appear to have a role in relieving symptoms, altering inflammatory status and boosting immunity in post covid patients.

Official Title
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Effect of Laser Acupuncture on Immunological Response and Dyspnea in Post Covid Syndrome Patients

Conditions
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Post Covid Syndrome

Intervention / Treatment
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* Device: laser acupuncture


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age will be ranged from 30 to 40 years patients were diagnosed as covid 19 by polymerase chain reaction test using nasopharyngeal swap patients will be negative polymerase chain reaction test (pcr) with symptoms of dyspnea and fatigue. patients will be recruited after 2 weeks post the negative pcr patients who want to participate and complete the study till the end Exclusion Criteria: patients with photosensitivity pacemaker plantation pregnant women BMI more than 30kg/m2 infection or inflammation at the site of acupuncture laser malignancy uncontrolled diabetes psychiatric disorders or mental retardation

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: group(1)<br>40 post covid 19 patients will receive laser acupuncture | Device: laser acupuncture<br>* gallium-aluminum arsenide diode laser with a continuous wave and a wavelength of 850nm will be used for laser acupuncture. it will be applied for three times per week for 12 weeks<br>|
| Placebo Comparator: group(2)<br>40 post covid patients will receive placebo laser acupuncture in which laser will be off | Device: laser acupuncture<br>* gallium-aluminum arsenide diode laser with a continuous wave and a wavelength of 850nm will be used for laser acupuncture. it will be applied for three times per week for 12 weeks<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in the level of CD 3 T cells | 5ml of blood will be drawn and serum will be separated. cd3 level will be measured using BD FACS Canto flow cytometer | after 12 weeks of treatment | 
| change in the level of cd4 T cells | cd4 will be measured using BD FACS Canto flow cytometer after blood sample will be separated | after 12 weeks of treatment | 
| change in level of interleukin 4 | BD FACS Canto flow cytometer with BD CBA human th1/th2 cytokine kit will be used | after 12 weeks of treatment | 
| change in level of interleukin 6 | BD FACS Canto flow cytometer with BD CBA human th1/th2 cytokine kit will be used | after 12 weeks of treatment | 
| change in lymphocyte count | BD FACS Canto flow cytometer will be used | after 12 weeks of treatment | 
| change in level of dyspnea | mMRC scale is a five point scale to detect degree of dyspnea while performing physical activity. it is ranged from 0-4 | after 12 weeks of treatment | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in fatigability | chalder fatigue scale is 11 items scale. people of 4 or more are considered meeting the criteria of fatigue | after 12 weeks of treatment |