Matrilysin Expression in Different Stages of Colorectal Tumors

Study Overview
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Brief Summary
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Matrix metalloproteinases (MMPs) have been shown to be involved in cancer biology. Significant expression of MMP-7 (matrilysin) in colorectal cancer is mainly associated with metastatic disease even though it is expressed in most tumor states. Our purpose is to analyse MMP-7 in bowel and lymph nodes of different tumor stages and to evaluate its expression as a potential biomarker of cancer disease in patients surgically treated for benign and malignant colorectal tumors. Tumoral tissue, lymph nodes and serum samples from recruited Patients plus serum samples from healthy volunteers are analysed for matrilysin expression by histology, immunohistochemistry, ELISA and Western blotting. If Matrilysin increases with increasing dysplasia and cancer disease stage in tumor tissue as well as in the regional lymph nodes it might be used as a complement in investigating suspected locally advanced cancer.

Official Title
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Matrilysin Expression in Different Stages of Colorectal Tumors

Conditions
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Colorectal Cancer Stage 0, Colorectal Cancer Stage I, Colorectal Cancer Stage II, Colorectal Cancer Stage III, Colorectal Cancer Stage IV

Intervention / Treatment
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* Procedure: colonic or colorectal resection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: benign colonic neoplasm not suitable for endoscopic treatment, malignant colonic neoplasm with indication to primary surgical treatment Exclusion Criteria: neo-adjuvant radiotherapy, chemo-radiotherapy, language problems and withdrawal of consent

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 83 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| healthy volunteers<br>healthy subjects not affected by benign or malignant colonic disease | Procedure: colonic or colorectal resection<br>* standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection<br>|
| benign colonic tumor patients<br>patients affected by benign colonic tumor such as adenoma | Procedure: colonic or colorectal resection<br>* standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection<br>|
| cancer patient I-II stage<br>Patients affected by colonic cancer in I-II stage | Procedure: colonic or colorectal resection<br>* standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection<br>|
| cancer patients III-IV stage<br>Patients affected by colonic cancer in III-IV stage | Procedure: colonic or colorectal resection<br>* standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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matrilysin, MMP-7, colorectal cancer, adenoma, lymph nodes