Resistance Exercise in Healthy Men

Study Overview
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Brief Summary
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In the present study, the effects of age and dietary intake will be assessed on myocellular characteristics during 72 hours after a resistance type exercise session.

Official Title
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The Effect of Age and Dietary Protein Intake on Myocellular Characteristics Following a Single Bout of Resistance Exercise in Healthy Men

Conditions
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Single Bout of Resistance Type Exercise, Standardized Diet

Intervention / Treatment
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* Behavioral: Adhere to a specific standardized diet for 4 days


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy men between the ages of 18-30 or 70-85 years Exclusion Criteria: Subjects with a recent history or current state of cardiovascular disease, COPD, Parkinson, rheumatoid arthritis, musculoskeletal/orthopaedic disorders, renal disorder, cognitive impairment.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Young regular diet<br>During the time of the study this group will adhere to a standardized regular diet. | Behavioral: Adhere to a specific standardized diet for 4 days<br>* 24 h before the start of the intervention all subjects will adhere to a standardized diet for 4 days. Young subjects are randomly allocated to either a low protein diet, regular protein diet or high protein diet group. All elderly subjects will adhere to a regular protein diet comparable to the regular protein diet of the young subjects.<br>|
| Experimental: Young low protein diet<br>During the time of the study this group will adhere to a standardized low protein diet. | Behavioral: Adhere to a specific standardized diet for 4 days<br>* 24 h before the start of the intervention all subjects will adhere to a standardized diet for 4 days. Young subjects are randomly allocated to either a low protein diet, regular protein diet or high protein diet group. All elderly subjects will adhere to a regular protein diet comparable to the regular protein diet of the young subjects.<br>|
| Experimental: Young high protein<br>During the time of the study this group will adhere to a standardized high protein diet. | Behavioral: Adhere to a specific standardized diet for 4 days<br>* 24 h before the start of the intervention all subjects will adhere to a standardized diet for 4 days. Young subjects are randomly allocated to either a low protein diet, regular protein diet or high protein diet group. All elderly subjects will adhere to a regular protein diet comparable to the regular protein diet of the young subjects.<br>|
| Experimental: Elderly<br>During the time of the study this group will adhere to a standardized high protein diet | Behavioral: Adhere to a specific standardized diet for 4 days<br>* 24 h before the start of the intervention all subjects will adhere to a standardized diet for 4 days. Young subjects are randomly allocated to either a low protein diet, regular protein diet or high protein diet group. All elderly subjects will adhere to a regular protein diet comparable to the regular protein diet of the young subjects.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle fiber myonuclear and stem cell content |  | baseline | 
| Muscle fiber myonuclear and stem cell content |  | 12 hours after a single bout of resistance type exercise | 
| Muscle fiber myonuclear and stem cell content |  | 24 hours after a single bout of resistance type exercise | 
| Muscle fiber myonuclear and stem cell content |  | 48 hours after a single bout of resistance type exercise | 
| Muscle fiber myonuclear and stem cell content |  | 72 hours after a single bout resistance type exercise |