Repair, Remodeling and Regeneration of the Bronchial Epithelium of COPD Patients

Study Overview
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Brief Summary
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COPD is characterized by exagerated decline FEV1 related to obstructive non reversible airflow. This could be the consequence of structural changes and inflammatory pattern of the bronchial wall. Lesions could lead to normal but also abnormal remodeling specially in COPD including a decrease in Club cells number and function.There is no treatment actually available targeted to a normal repair of the epithelium. The objective of this work is to identify potential targets for reprograming bronchial epithelial cells I order to achieve a good repair.

Official Title
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Repair, Remodeling and Regeneration of the Bronchial Epithelium of COPD Patients

Conditions
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Heathy Volunteers, Smokers, COPD

Intervention / Treatment
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* Other: In vitro experiments on bronchial epithelial cells


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: COPD - Smokers or ex smokers > 30p/yr FEV1/FVC < 0.7 With a fibroscopy prescription Smokers - Smokers or ex smokers > 30p/yr FEV1/FVC > 0.7 With a fibroscopy prescription Controls - age superior or equal to 18 no smoker (for 5 years) with a fibroscopy prescription Exclusion Criteria: Xylocaine hypersensibility Porphyria severe hepatic failure Epilepsy Severe cardiac failure

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Heathy volunteers, smokers and COPD<br> | Other: In vitro experiments on bronchial epithelial cells<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Full recovery one day after injury (yes / no) proportion of cells showing the repair, remodeling or regeneration of the epithelium (ciliated cells, mucus cells, Clara cells) in culture in air-liquid interface in the three groups | The study will be judged on the proportion of cells showing the repair, remodeling or regeneration of the epithelium (ciliated cells, mucus cells, Clara cells) in culture in air-liquid interface in the three groups. | 1 day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurement of apoptosis and inflammation (IL8) | Determine the molecular mechanisms and deleterious cell leading to poor repair of the epithelium in patients with COPD | 24 months | 
| Measure the effect of the CC10 protein secreted by Clara cells on the repair of the bronchial epithelium. | Measuring the reprogramming of the epithelium of COPD patients to a control-type epithelium | 24 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Bronchial epithelium, COPD, Repair, Remodelling, Club Cells