Thiol/Disulphide Homeostasis and Vascular Endothelial Growth Factor Levels in Diabetic Children

Study Overview
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Brief Summary
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The aim of this study to evaluate the vascular endothelial growth factor level and thiol/disulphide homeostasis in saliva of diabetic children with gingivitis.

Detailed Description
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Eighty children (44 with type 1 diabetes mellitus and 44 systemically healthy) will be recruited for the study. The children will be divided into four subgroups based on their periodontal and systemic condition: 1) systemically and periodontally healthy children, 2) systemically healthy children with gingivitis, 3) periodontally healthy children with T1DM, and 4) children with T1DM and gingivitis. The plaque index, gingival index, probing pocket depth, will recorded. The vascular endothelial growth factor level determined by ELISA. Native thiol, total thiol and disulphide levels will be measured in saliva.

Official Title
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Thiol/Disulphide Homeostasis and Salivary Vascular Endothelial Growth Factor Levels in Type 1 Diabetic Patients With Gingivitis

Conditions
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Periodontal Diseases

Intervention / Treatment
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* Diagnostic Test: ELISA


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Children diagnosed as type 1 diabetes mellitus Age between 7-14 years Exclusion Criteria: Having another systemic problem except type 1 diabetes mellitus Children carrying orthodontic appliances

Ages Eligible for Study
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Minimum Age: 7 Years
Maximum Age: 14 Years

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Type 1 diabetes with gingivitis<br>Children with type 1 diabetes mellitus and gingivitis | Diagnostic Test: ELISA<br>* Saliva sample collection<br>* Other names: Total thiol, native thiol and disulphide level;|
| Type 1 diabetes with healthy gingiva<br>Children with type 1 diabetes mellitus and healthy gingiva | Diagnostic Test: ELISA<br>* Saliva sample collection<br>* Other names: Total thiol, native thiol and disulphide level;|
| Healthy children with gingivitis<br>Systemically healthy children with gingivitis | Diagnostic Test: ELISA<br>* Saliva sample collection<br>* Other names: Total thiol, native thiol and disulphide level;|
| Healthy children and healthy gingiva<br>Systemically healthy children with healthy gingiva | Diagnostic Test: ELISA<br>* Saliva sample collection<br>* Other names: Total thiol, native thiol and disulphide level;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Vascular endothelial growth factor level | Vascular endothelial growth factor level in saliva | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Thiol/disulphide measurement | Total thiol, native thiol and disulphite level in saliva | Baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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type 1 diabetes mellitus