Distribution of Nutrient Derived Amino Acids

Study Overview
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Brief Summary
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The project examines over several days how the distribution pattern of dietary protein stimulate and affect the protein turnover of important proteins in the aging perspective, such as skeletal muscle proteins.

Official Title
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Distribution of Nutrient Derived Amino Acids and Anabolic Effect in Healthy Elderly Individuals

Conditions
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Muscle Mass, Protein Intake, Diet Modification, Protein Synthesis, Protein Breakdown, Sarcopenia

Intervention / Treatment
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* Other: Distribution pattern of dietary proteins (minced meat)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Men and women Between 65-80 years of age Exclusion Criteria: BMI above 30 Smokers Vegetarians Diabetes or other metabolic diseases Medical treatment which affects protein turnover Gastro-intestinal diseases, reduced kidney or liver function, or chronic inflammation diseases Elevated blood-pressure or signs of atherosclerosis More than 5 hours of regular exercise each week

Ages Eligible for Study
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Minimum Age: 65 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: Randomized controlled
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: MINCED-SKEWED<br>Minced meat administered with a skewed protein distribution pattern | Other: Distribution pattern of dietary proteins (minced meat)<br>* Study effect of an even or skewed distribution pattern of dietary protein<br>|
| Experimental: MINCED-EVEN<br>Minced meat administered with an even protein distribution pattern | Other: Distribution pattern of dietary proteins (minced meat)<br>* Study effect of an even or skewed distribution pattern of dietary protein<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein synthesis | Myofibrillar FSR measured by deuterated water technique, by which deuterium labeled alanine incorporation into myofibrillar muscle protein synthesis can be measured. | 3 days | 
| Net protein balance | Whole body net protein balance measured during 10 hours acute study. Measured by the application of stable amino acid labeled phenylalanine and tyrosine tracers, by which the protein synthesis, phenylalanine oxidation and protein breakdown can be calculated. | 10 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| D5-phenylalanine incorporation | D5-phenylalanine incorporation into myofibrillar proteins. Dietary meat proteins are intrinsically labeled with D5-phenylalanine | 3 days | 
| Muscle protein synthesis | Myofibrillar protein synthesis (%/hour) measured during 10 hours of the acute study. Calculated from the difference in D8-phenylalanine incorporation from the muscle biopsies taken at 2 hours and 12 hours into the stable amino acid tracer infusion. | 10 hours | 
| Protein synthesis | Whole body protein synthesis (%/hour) measured during acute study, by the use of stabel amino acid tracer technique. | 10 hours | 
| Protein breakdown | Whole body protein breakdown (%/hour) measured during acute study, by the use of stabel amino acid tracer technique. | 10 hours | 
| Phenylalanine oxidation | Phenylalanine oxidation measured during acute study | 10 hours | 
| D5-phenylalanine appearance | Plasma D5-phenylalanine enrichment after intake of D5-phenylalanine intrinsically labelled meat proteins | 12 hours | 
| Signaling pathways in skeletal muscle | Intracellular signaling pathways related to protein turnover. Measured by Western blotting technique from the muscle tissue biopsies taken at 2 hours and 12 hours into the acute study. | 10 hours | 
| Plasma insulin | Plasma insulin concentration during acute study | 12 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Dietary protein, Sarcopenia, Protein turnover, Muscle protein