Study of Enzalutamide in Patients With Castration-resistant Prostate Cancer

Study Overview
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Brief Summary
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This research study is evaluating a drug called enzalutamide in metastatic castration resistant prostate cancer. Enzalutamide is already FDA approved for metastatic castration resistant prostate cancer after treatment with chemotherapy. The purpose of this study is to analyze features of tumor specimens sampled prior to therapy and at disease progression to determine why patients respond or stop responding to treatment with Enzalutamide. Prior chemotherapy is not a requirement of this trial.

Detailed Description
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After the screening procedures confirm that the patient is able to participate in the study, The patient will have the first of two required prostate biopsies prior to starting study treatment. The patient will be given a prescription for study drug and a study drug-dosing diary for each treatment cycle. Each treatment cycle lasts 28 days (4 weeks), during which time the patient will be taking the study drug once daily. The diary will also include special instructions for taking the study drug. The study drug (enzalutamide) should be taken orally (by mouth) at home. On Day 1 of each cycle (+/- 4 days), the following procedures will be performed in clinic: A medical history. A Physical examination Performance status Blood tests (2-3 tablespoons). The patient will be asked about medications they are currently taking, including over-the counter medications, herbal remedies, vitamins, and supplements. The patient will be asked about any disease-related symptoms they are experiencing. The patient will receive a new supply and the medication diary will be reviewed. Every 12 weeks (+/-1 week) the following procedures will be performed in clinic: Blood tests. A small sample of the patient's blood (about 1-2 tablespoons) will be collected. This blood will be collected for specialized laboratory tests. A (CT) scan of the patient's chest and a CT or MRI scan of abdomen, and pelvis and a bone scan will be performed every 12 weeks (+/- 1 week) while the patient is on active treatment. If your baseline CT of the patient's chest does not show disease, the investigator may not asked for this to be repeated. End of Study Visit in clinic: A medical history. A physical examination Performance status Blood tests (2-3 tablespoons) The patient will be asked about any disease-related symptoms If the patient completed at least 4 cycles of enzalutamide, the patient will undergo a second of two required biopsies.

Official Title
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Phase II Trial of Enzalutamide for Castrate-resistant Prostate Cancer (CRPC) With Correlative Assessment of Androgen Receptor (AR) Signaling and Whole-exome and Transcriptome Sequencing

Conditions
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Metastatic Castration-resistant Prostate Cancer

Intervention / Treatment
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* Drug: Enzalutamide


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Participants must meet the following criteria on screening examination to be eligible to participate in the study: Be a male ≥ 18 years of age. Participants must have histologically or cytologically confirmed adenocarcinoma of the prostate without ≥50% neuroendocrine differentiation or small cell histology. Participants must have progressive disease as defined by either: Castrate resistant disease as defined by PCWG. Participants must have a rise in PSA on two successive determinations at least one week apart and PSA levels ≥ 2 ng/ml (only the screening PSA needs to be ≥ 2 ng/ml) and testosterone levels < 50 ng/dL, OR Soft tissue progression defined by RECIST 1.1, OR Bone disease progression defined by PCWG2 with two or more new lesions on bone scan. CRPC with metastatic disease with at least one site of metastatic disease must be amenable to needle biopsy. Soft tissue biopsy sites include: lymph node or visceral metastases. Bone sites include lumbar vertebrae, pelvic bones and long bones. Excluded sites are thoracic, cervical vertebrae, skull and rib lesions. Biopsy site will be selected with guidance of interventional radiologist determining best site to optimize balance of obtaining useful tissue for analysis and minimizing risk. Participants without orchiectomy must be maintained on LHRH agonist/antagonist therapy. Participants may have had any number of previous hormonal therapies (antiandrogens, steroids, estrogens, finasteride, dutasteride, ketoconazole, abiraterone) provided these were discontinued ≥ 4 weeks before enrollment. Participants may have had up to two previous cytotoxic therapeutic regimens provided these were discontinued ≥ 4 weeks before enrollment. At least a 4 week interval from previous prostate cancer treatment other than LHRH agonist/antagonist therapy or bisphosphonates to enrollment. Participants receiving bisphosphonates therapy can be maintained on this therapy. If participants have not started bisphosphonates, it is recommended that they start treatment after the first biopsy. ECOG performance status < 2 (Karnofsky >60%, see Appendix A). Participants must have normal organ and marrow function as defined below: WBC ≥ 3,000/mcL ANC ≥ 1,500/mcL Platelets ≥ 100,000/mcL Hemoglobin ≥ 9 g/dL Serum albumin ≥ 3.0 g/dL AST, ALT, and total bilirubin ≤ 1.5 x Institutional ULN Creatinine ≤ 1.5 Institutional ULN or a calculated creatinine clearance ≥ 50 mL/min using the Cockcroft Gault equation PTT ≤ 60, INR ≤ 1.5 Institutional ULN unless on warfarin therapy (investigator would need to determine if safe for participant to stop warfarin prior to biopsy) Have signed an informed consent document indicating that the subjects understands the purpose of and procedures required for the study and are willing to participate in the study. Be willing/able to adhere to the prohibitions and restrictions specified in this protocol. Written Authorization for Use and Release of Health and Research Study Information (US sites only) has been obtained. Able to swallow the study drug whole as a tablet. Participants who have partners of childbearing potential must be willing to use a method of birth control with adequate barrier protection as determined to be acceptable by the principal investigator during the treatment period and for 1 week after last dose of enzalutamide. Exclusion Criteria: Participants who exhibit any of the following conditions at screening will not be eligible for admission into the study. Uncontrolled illness including, but not limited to ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements within 6 months of enrollment. Clinically significant heart disease as evidenced by: Myocardial infarction within 6 months of enrollment. Uncontrolled angina within 6 months of enrollment. Congestive heart failure NYHA Class III or IV, or a history of congestive heart failure NYHA Class III or IV in the past, unless a screening ECHO or MUGA within 3 months results in a left ventricular ejection fraction ≥ 45%. Clinically significant ventricular arrhythmias. History of Mobitz II second degree or third degree heart block without a permanent pacemaker in place. Bradycardia as indicated by a heart rate < 50 beats per minute at screening visit. Hypotension as indicated by SBP ≤ 85 on 2 consecutive measurements. Uncontrolled hypertension as indicated by SBP > 170 mmHg or DBP > 105 mmHg on 2 consecutive measurements at screening visit. Thromboembolism within 6 months of enrollment. History of gastrointestinal disorders (medical disorders or extensive surgery) which may interfere with the absorption of the study drug. History of seizure or any condition or concurrent medication that may predispose to seizure. Individuals with a history of a different malignancy are ineligible except for the following circumstances: 1) individuals with a history of other malignancies are eligible if they have been disease-free for at least 5 years and are deemed by the investigator to be at low risk for recurrence of that malignancy, or 2) individuals with the following cancers are eligible if diagnosed and treated within the past 5 years: superficial bladder cancer, basal cell or squamous cell carcinoma of the skin. Known brain metastasis. Participants with brain metastasis can only be included if they were treated > 4 week prior to enrollment with radiation and the effects of treatment have resolved. Subjects with treated brain metastases must have a post-treatment brain MRI showing no further progression of prior lesions and no new metastatic lesions. Subjects will be ineligible if they have any ongoing symptoms from brain metastases or if there continues to be radiographic evidence of cerebral edema. Have known allergies, hypersensitivity, or intolerance to enzalutamide or their excipients. Surgery or local prostatic intervention within 30 days of the first dose. In addition, any clinically relevant issues from the surgery must have resolved prior to enrollment. Major surgery or radiation therapy within 4 weeks of enrollment. Radium-223, strontium-89, or samarium-153 therapy within 4 weeks of enrollment. Radiotherapy, chemotherapy or immunotherapy within 4 weeks, or single fraction of palliative radiotherapy within 14 days of administration of enrollment. Prior treatment with enzalutamide. Current enrollment in an investigational drug or device study or participation in such a study within 30 days of enrollment. Concomitant use of medications that may alter pharmacokinetics of enzalutamide. See section 5.3. Any acute toxicities due to prior chemotherapy and/or radiotherapy that have not resolved to a NCI CTCAE (version 4) grade of ≤ 1. Chemotherapy induced alopecia and grade 2 peripheral neuropathy are allowed. Condition or situation which, in the investigator's opinion, may put the participant at significant risk, may confound the study results, or may interfere significantly with participant's participation in the study. Individuals not willing to comply with the procedural requirements of this protocol. HIV-positive individuals on combination antiretroviral therapy are ineligible because of the potential for pharmacokinetic interactions with enzalutamide. Appropriate studies will be undertaken in participants receiving combination antiretroviral therapy when indicated.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Enzalutamide<br>Administration: 160 mg orally once daily with a 28-day cycle. Treatment will be continued until evidence of symptomatic or radiographic progression or the participant is taken off the study for another reason. Dosing: 160 mg orally taken once daily as four 40 mg capsules. | Drug: Enzalutamide<br>* Enzalutamide, formerly known as MDV3100, is a rationally-designed second generation AR inhibitor which functions by blocking several steps in the AR signaling cascade. Enzalutamide competitively binds the AR with great potency. Additionally, enzalutamide inhibits nuclear translocation of activated AR and inhibits the association of activated AR with DNA.<br>* Other names: Xtandi;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of Patients With Gene Alterations | Serial biopsies was performed at pre-study and progression to analyze mechanisms of resistance to enzalutamide, such as alterations in AR (mutations, amplifications), tumor suppression genes, DNA repair genes, and SPOP. Whole exome sequencing was performed on tumor tissue biopsies. | Tumor biopsies were performed at pre-study and progression. Progression occurred up to 41 months after initiation of enzalutamide. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in Serum Androgen Concentrations Between Baseline and Subsequent Assessment Visits | Serum testosterone were be collected at pre-study (C1D1) and every cycle and changes over time from baseline were planned to be summarized descriptively. | Not collected. | 
| Number of Participants With a PSA Response | PSA response is defined per PCWG2 criteria (PSA decline >=50% relative to cycle 1 PSA) and is summarized as frequency and percent. | PSA was measured at pre-treatment, each C1D1 (1 cycle=28 days), end of treatment, and follow-up visits (every 6 months). PSA were measured up to 52 months. | 
| Duration of PSA Response | Duration of PSA response is defined as time from the first achievement of PSA response (PSA decline >=50% from cycle 1) to the date of PSA progression or death, whichever occurs first. | PSA was measured at pre-treatment, each C1D1 (1 cycle=28 days), end of treatment, and follow-up visits (every 6 months). PSA were measured up to 52 months. | 
| Response of Measurable Disease at Baseline | Objective responses for measurable disease was evaluated using RECIST 1.1 criteria. Complete Response (CR): Disappearance of all target lesions. Any pathological lymph nodes must have reduction in short axis to <10 mm. Partial Response (PR): At least a 30% decrease in the sum of the diameters of target lesions, taking as reference the baseline sum diameters. Response was tabulated with frequency. | Imaging was performed at pre-study and every 12 weeks on treatment. Treatment cycles is 28 days. Patients were assessed up to 41 months. | 
| Duration of Response of Measurable Disease | Duration of response of measurable disease is defined as time from the date of first objective response (PR/CR) to date of progression or death, whichever comes first. Objective response is per RECIST 1.1 criteria: Complete Response (CR): Disappearance of all target lesions. Any pathological lymph nodes must have reduction in short axis to <10 mm. Partial Response (PR): At least a 30% decrease in the sum of the diameters of target lesions, taking as reference the baseline sum diameters. | Imaging was performed at pre-study and every 12 weeks on treatment. Treatment cycles is 28 days. Patients were assessed up to 41 months. | 
| Toxicity Measurements | For safety and tolerability, treatment-related grade 3 or higher toxicities was assessed using CTCAE v. 4.0 and was provided using frequency. | Toxicity was reported at pre-study, every cycle, and end of treatment visit. Patients reported toxicities up to 39 months. | 
| Number of Participants With PSA Response Stratified by Gene Alterations in Serial Tumor Biopsies | Serial biopsies was performed at pre-study and progression to analyze mechanisms of resistance to enzalutamide, such as alterations in AR (mutations, amplifications), DNA repair genes, and SPOP. Whole exome sequencing was performed on tumor tissue biopsies. Gene alterations were tabulated with PSA response. | Tumor biopsies will be performed at pre-treatment and end of treatment visit. Treatment cycle is 28 days. PSA was collected up to 52 months. | 
| Subsequent Lines of Therapy | Subsequent lines of therapy following study drug discontinuation was summarized descriptively. | Subsequent treatments were followed up to 31 months after discontinuation of study drug. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Enzalutamide, Metastatic Castration-resistant Prostate Cancer