Study of Let-7a and miRNA-124 in Cases of Non-Hodgkin's Lymphoma and Acute Leukemia

Study Overview
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Brief Summary
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MiRNAs are noncoding RNAs (ncRNAs) that drive post-transcriptional negative regulation of gene expression by promoting the degradation or translational blockade of their target mRNAs. MiRNAs are 21-24-nucleotide-long RNA molecules that are processed from longer RNA precursors (pri-miRNAs) , and either the 5' or the 3' strand of the mature miRNA duplex is loaded into the Argonaute (AGO) family of proteins to form a miRNA-induced silencing complex (miRISC) When bound to AGO proteins, mature miRNAs destabilize or inhibit the translation of partially complementary target mRNAsMiRNA-124 has been shown to be a tumor suppressor, and a decrease in its expression level is typical of tumors of various localization, but there is no evidence of the role of miRNA-124 in the development of NHL . There are a number of studies reflecting the involvement of miRNA-124 in hematopoiesis. Liu et al. showed that miRNA-124 regulates Tip110 that is involved in the differentiation of hematopoietic stem cellsThe let-7 family has been shown to influence the pathogenesis of a variety of hematological malignancies through the changing expression of a number of oncogenic pathways, particularly those related with MYC and that might affect hematopoietic carcinogenesis through the modulation of inflammatory pathways

Official Title
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Study of Let-7a and miRNA-124 in Cases of Non-Hodgkin's Lymphoma and Acute Leukemia

Conditions
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Non Hodgkin Lymphoma and Acute Leukemia

Intervention / Treatment
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* Diagnostic Test: real time pcr


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: approval to sign an informed written consent, patient with NHL and acute leukemia . Exclusion Criteria: Refusal to sign an informed written consent, patient on chemotherapy

Ages Eligible for Study
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Minimum Age: 1 Year
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: control group<br>the patients with non-malignant hematological diseases | Diagnostic Test: real time pcr<br>* Detection of expressin level of MiRNA-124 and let-7a using real time pcr<br>|
| Active Comparator: the cases (A)<br>NHL . | Diagnostic Test: real time pcr<br>* Detection of expressin level of MiRNA-124 and let-7a using real time pcr<br>|
| Active Comparator: the cases (B)<br>acute leukemia | Diagnostic Test: real time pcr<br>* Detection of expressin level of MiRNA-124 and let-7a using real time pcr<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| MiRNA-124 level | Detection of expressin level of MiRNA-124 IN Non-Hodgkin's Lymphoma and acute leukemia | 6 months | 
| let-7a level | Detection of expressin level of let-7a IN Non-Hodgkin's Lymphoma and acute leukemia | 6 months |