Serum Amyloid A in Women With Unexplained Infertility

Study Overview
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Brief Summary
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High follicular fluid amyloid A level is associated with reduced pregnancy rate. The aim of this study was to find an association between serum level of amyloid A and unexplained infertility.

Official Title
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Serum Amyloid A Level in Women With Unexplained Infertility

Conditions
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Infertility

Intervention / Treatment
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* Other: Serum amyloid A level


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: women with unexplained infertility (unprotected, regular intercourse for at least 2 years). Nulligravida ,Regular mensturation. Age less than 40 years . Normal parameters (in the study group): hormonal profile (day 2 FSH,LH), midluteal phase progesterone, Prolactin, TSH transvaginal ultrasound hysterosalpingography detailed semen analysis Exclusion Criteria: Age above 40 Smoking females Obesity (BMI>30) Women with any systemic or medical diseases leads to elevation of SAA as: Systemic lupus erythematosis Rheumatoid arthritis Sarcoidosis Diabetes Mellitus Using hormonal contraception (for control group)

Ages Eligible for Study
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Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Study<br>Women with unexplained infertility | Other: Serum amyloid A level<br>* For determination of SAA level, a 5-ml sample of venous blood will be taken from each participant under aseptic conditions, and centrifuged at 2500 g for 15 minutes at 4°C c. separated into serum aliquots,and stored frozen at -80°C until analysis of SAA ,which will be done by a double-antibody sandwich Enzyme Linked Immunosorbent Assay (ELISA) kits supplied by cusabio,USA according to manufacturers instruction .Each sample was tested in duplicate and the average will be taken. A cut off level of 30 mg/L SAA level will be chosen to reflect a real increase in its level<br>|
| Control<br>Fertile women who had normal physical and pelvic examination, regular menstrual cycles, don't use hormonal contraceptive, had one child at least. | Other: Serum amyloid A level<br>* For determination of SAA level, a 5-ml sample of venous blood will be taken from each participant under aseptic conditions, and centrifuged at 2500 g for 15 minutes at 4°C c. separated into serum aliquots,and stored frozen at -80°C until analysis of SAA ,which will be done by a double-antibody sandwich Enzyme Linked Immunosorbent Assay (ELISA) kits supplied by cusabio,USA according to manufacturers instruction .Each sample was tested in duplicate and the average will be taken. A cut off level of 30 mg/L SAA level will be chosen to reflect a real increase in its level<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Difference between SAA level between cases and control. |  | 1 day (once) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Correlation between SAA level with other parameters in the hormonal profile (e.g., FSH, LH, E2). |  | 1 day (once) |