Impact of Non Digestible Carbohydrate on Production of Phenolic Acids From Strawberry Juice

Study Overview
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Brief Summary
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This is an acute human bioavailability study in self-reported healthy participants aged 20-70 years old. We hypothesize that combination of dietary polyphenolics and non-digestible carbohydrates (NDC) will increase the production of phenolic acids by bacteria in the human colon and these will be detected in urine. Participants will attend for three arms in a randomised order: Strawberry juice (a high polyphenol food), Inulin (NDC) or Mixture of strawberry juice and inulin.

Detailed Description
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Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols. The colonic microbiota are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as dietary fibre to short chain fatty acids. It is likely that there are key interactions in the colonic bacteria metabolism of fibre and phenolics. We hypothesize that combination of polyphenolics and non-digestible carbohydrates (NDC) will increase the urinary output of bioactive phenolic acids. This study will enable a better understanding of how to deliver combinations of ingredients and nutrients to achieve maximum nutritional value and health benefits.

Official Title
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Impact of Non Digestible Carbohydrate on Production of Phenolic Acids From Strawberry Juice

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Strawberry juice with Inulin
* Dietary Supplement: Strawberry Juice
* Dietary Supplement: Inulin


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Self-reported healthy adults Exclusion Criteria: Antibiotic use within the last 3 months, identified gastro-intestinal diseases, on prescribed medication other than the contraceptive pill, individuals who are pregnant or breastfeeding. Individuals who have been diagnosed as anaemic, as well as those who are allergic to any food, or paracetamol.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Acute human bioavailability studies with a cross-over design will be given to subjects in the form of a drink in one of three combinations Strawberry juice alone Inulin alone Mixture of strawberry juice and inulin
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Strawberry juice with inulin<br>One dose of 300 g of strawberry with 10 g of inulin will be given to subjects in the form of juice | Dietary Supplement: Strawberry juice with Inulin<br>* Mixture of polyphenols and non digestible carbohydrates<br>|
| Experimental: Strawberry juice<br>One dose of 300 g of strawberry juice will be given to subjects in the form of juice | Dietary Supplement: Strawberry Juice<br>* Source of Polyphenols<br>|
| Experimental: Inulin<br>One dose of 10 g of inulin will be given to subjects in the form of a drink | Dietary Supplement: Inulin<br>* Source of non digestible carbohydrate<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Phenolic acids bioavailability | Urine excretion of phenolic acids | 0-24 hrs | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Urolithin bioavailability | Urine excretion of urolithins | 0-24 hrs | 
| Glycaemic and appetite hormones measurements by ELISA | Plasma glucose, insulin and appetite hormone levels | 0-8 hrs | 
| Mouth to caecum transit time | Breath hydrogen level measurements by Hydrogen monitor | 0-8 hrs | 
| Gastric emptying time | Plasma paracetamol levels by acetaminophen assay kits | 0-6 hrs | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Self-reported healthy adults