Thiol Disulfide Balance in Cervix Cancer

Study Overview
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Brief Summary
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The aim of this research is to evaluate thiol-disulfide balance in cervix cancer patients

Detailed Description
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To date various oxidative stress parameters were studied in patients with cervix cancer, however dynamic thiol disulfide hemostasis has not been previously investigated. The thiol-disulfide balance in the circulation will be measured by the automated system created by Erel & Neselioglu (1). References 1- Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32. doi:10.1016/j.clinbiochem.2014.09.026.

Official Title
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Evaluation of Thiol Disulfide Balance in Cervix Cancer

Conditions
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Cervix Cancer, Oxidative Stress

Intervention / Treatment
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* Diagnostic Test: Disulphide(μmol/L)
* Diagnostic Test: Total Oxidant Capacity(μmolH2O2Equiv/L)
* Diagnostic Test: Ischemia Modified Albumin(IU/mL)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: • Histopathologically confirmed diagnosis of cervix cancer Exclusion Criteria: pulmonary disease pulmonary hypertension cardiac dysfunction renal dysfunction liver disease chronic ischemia systemic inflammation concomitant malignancy patients who use vitamin A,C or E (antioxidant vitamins) patients who use smoke patients who use drink alcohol patients who use addictive for any drug

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Cervix cancer<br>Cervix cancer n=62 | Diagnostic Test: Disulphide(μmol/L)<br>* Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry<br>* Other names: Disulfide/total thiol(%);Diagnostic Test: Total Oxidant Capacity(μmolH2O2Equiv/L)<br>* Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry<br>* Other names: Oxidative stress Index (Arbitrary Unite);Diagnostic Test: Ischemia Modified Albumin(IU/mL)<br>* The serum Ischemia Modified Albumin concentrations were measured as described by Bar-Or et al. (Bar-Or et al. 2000).<br>|
| Healthy controls<br>Healthy volunteers n=61 | Diagnostic Test: Disulphide(μmol/L)<br>* Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry<br>* Other names: Disulfide/total thiol(%);Diagnostic Test: Total Oxidant Capacity(μmolH2O2Equiv/L)<br>* Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry<br>* Other names: Oxidative stress Index (Arbitrary Unite);Diagnostic Test: Ischemia Modified Albumin(IU/mL)<br>* The serum Ischemia Modified Albumin concentrations were measured as described by Bar-Or et al. (Bar-Or et al. 2000).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| serum native thiol level | serum native thiol level in μmol/L | preoperative in the morning or preprandial for healthy volunteers | 
| serum disulphide level | serum disulphide level in μmol/L | preoperative in the morning or preprandial for healthy volunteers | 
| serum total thiol level | serum total thiol level in μmol/L | preoperative in the morning or preprandial for healthy volunteers | 
| the disulphide/native thiol ratio | the disulphide/native thiol ratio X (100 ) | preoperative in the morning or preprandial for healthy volunteers | 
| disulphide/total thiol ratio | disulphide/total thiol ratio X (100 ) | preoperative in the morning or preprandial for healthy volunteers | 
| native thiol/total thiol ratio | native thiol/total thiol ratio X (100 ) | preoperative in the morning or preprandial for healthy volunteers | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| total oxidant capacity (TOC) | TOC in mmol H2O2 equivalent/L | preoperative in the morning or preprandial for healthy volunteers | 
| total antioxidant capacity (TAC) | TAC in mmol trolox equivalent/L | preoperative in the morning or preprandial for healthy volunteers | 
| Oxidative stress index (OSI) | OSI in mmol trolox/L. | preoperative in the morning or preprandial for healthy volunteers | 
| ischaemia-modified albumin | ischaemia-modified albumin in IU/mL | preoperative in the morning or preprandial for healthy volunteers | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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thiol disulfide hemostasis, screening, early diagnosis