Investigation of Fat Oxidation During Moderate Versus Vigorous Intensity Exercise

Study Overview
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Brief Summary
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Exercise is widely suggested as an important lifestyle change for weight loss, however, the optimal level of intensity moderate versus vigorous remains unclear. It is known that in athletes, during very high-intensity exercise, fat oxidation is decreased and energy is gained mainly from carbohydrate utilization. The aim of this study is to find an optimal workload based on fat oxidation rate during exercise in volunteers with a sedentary lifestyle.

Detailed Description
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It is planned to recruit 12 healthy volunteers with a sedentary lifestyle in the study group. Since different regimens (fed, fasted and moderate versus vigorous) will be compared in the same subjects, no control group is necessary. Subjects will perform a cycling test with 5 min incremental intensity exercise and 1 h cycling test exercising at a moderate-intensity (load will be selected after the incremental intensity exercise). The expiration of carbon dioxide (CO2) and consumption of oxygen (O2) will be measured, to determine the total fatty acid and carbohydrate oxidation during cycling. Concomitantly, it is planned to measure blood lactate and glucose concentrations during exercise experiments as well as a wide range of blood biochemistry parameters before and after exercise. The investigators expect to find optimal conditions for the sedentary population for burning of fat, which is the major source of energy during moderate-intensity exercise. Measurements of the fat and carbohydrate oxidation in healthy volunteers with a sedentary lifestyle will contribute to the understanding of exercise regimens for the most effective fat oxidation.

Official Title
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Investigation of Fat and Carbohydrate Oxidation During Moderate Versus Vigorous Intensity Exercise in Healthy Volunteers With Sedentary Lifestyle

Conditions
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Healthy, Sedentary Lifestyle

Intervention / Treatment
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* Other: Exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy Sedentary lifestyle BMI 20 - 30 kg/m2 Be able to attend the laboratory and willing to participate in necessary protocols Be willing to undertake the duration of the exercise protocol (3 x 1 hour) Have the capacity and willingness to provide informed consent (oral and written) Exclusion Criteria: athletes Individuals with chronic cardiovascular disease such as hypertension, valve disease, coronary artery disease, cardiac conduction abnormalities, etc. History of pneumothorax or chronic lung disease such as asthma, COPD, bronchiectasis Active Smokers Pregnant women

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 55 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Exercise<br>Subjects will perform a cycling tests | Other: Exercise<br>* Subjects will perform a cycling test with 5 min incremental intensity exercise and 1 h cycling test exercising at a moderate-intensity (load will be selected in incremental intensity exercise).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Optimal workload | The exercise workload (W) for optimal fat burning during exercise | 30 min accelerated load cycling exercise | 
| Heart rate change | Change in heart rate (BPM) from rest to the optimal workload | 30 min accelerated load cycling exercise and 60 min optimal intensity cycling exercise | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Influence of fed and fasted state | The influence of fed and fasted state on maximal whole body fat oxidation rate during exercise | 30 min accelerated load cycling exercise | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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exercise, fed, fasted, fat, carbohydrate