Gasdermin-D and Pannexin-1 in Pregnancy

Study Overview
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Brief Summary
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One -third of fertile women around the world are overweight or obese. This means increasing risk for both the mother and the child. Fat tissue is a factor in gestational DM development and the increase in material inflammation and oxidative stress. According to new knowledge, it is an important need to examine molecules that are not handled in new and human research in these mechanisms in fat and placenta tissues in obesity. For this purpose, the examination of the expression of gasdermin-D and pannex-1 proteins, which are shown to be produced in the cells of both tissues, is worth investigating in human fat tissue and placenta. Gasdermins and pannexins are proteins intersecting by interacting in cellular functions. Gasdermins cause piroptosis, a type of litic proinflammatory cell death. Pannexin-1 plays in various cellular functions, including inflammation. These are examined in a small number of in vitro studies in material fat tissue and placenta, and the design of this study in people whose applications are applied is original in humans. The status of the expressions of the gasdermin-D and pannexin-1 proteins, which will be examined for the first time in obese pregnant women's fat and placental tissues, are the subject of this research with each other and their relationship with other maternal and neonatal data.

Official Title
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Investigation of Gasdermin-D and Pannexin-1 in Fat and Placental Tissues in Pregnant Women With Obesity

Conditions
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Pregnancy Complications, Maternal Obesity

Intervention / Treatment
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* Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Being in the 20-35 age range Term pregnancy (37-41 weeks) Healthy pregnancy follow-up Planned cesarean section Having no complication in cesarean delivery Being a placenta with front or rear walls Exclusion Criteria: Intrauterine surgical procedure Presence of prenatal placental structural disorder Multiple pregnancies

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Normal Weight Pregnant Women<br>The women who had term pregnancy and had normal BMI. | Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D<br>* These biomarkers were measured in the placental and adipose tissues of the participants.<br>|
| Experimental: Overweight Pregnant Women<br>The women who had term pregnancy and had BMI values between 25-30. | Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D<br>* These biomarkers were measured in the placental and adipose tissues of the participants.<br>|
| Experimental: Obese Pregnant Women<br>The women who had term pregnancy, and had BMI values more than 30. | Diagnostic Test: Biomarker measurement of pannexin-1 and gasdermin-D<br>* These biomarkers were measured in the placental and adipose tissues of the participants.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Prediction of macrosomy | Prediction of macrosomy will be assesed by meaningfull increases of studied biomarkers Gasdermin-D and Pannexin-1 | 7 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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gasdermin-D, pannexin-1, placenta, omental tissue, fat tissue, maternal obesity, inflammation, oxidative stress, biochemistry, ELISA