HIV Fat Redistribution and the Evaluation of Brown Fat

Study Overview
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Brief Summary
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The specific aims of this study are to determine whether HIV-infected patients with significant fat redistribution and ectopic fat accumulation have increased brown adipose tissue using 18F-FDG Positron Emission Tomography techniques. Recent studies suggest down regulation of Dicer, a major component of miRNA has an important role in the differentiation and function of brown and white adipose tissue and may contribute to lipodystrophy. Therefore we will expand on recent research in this area by recruiting HIV-infected men with lipodystrophy. We will perform subcutaneous fat biopsies of the dorsocervical and abdominal fat in a subset of HIV-infected and non-HIV-infected men in order to explore further the question of down regulation of Dicer and its implication on metabolic abnormalities in this population.

Detailed Description
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Among individuals infected with HIV, highly active antiretroviral therapy has reduced the incidence of morbidity and mortality however, despite recent improvements in newer antiretrovirals patients continue to exhibit secondary effects related to body composition such as lipoatrophy of the periphery, increased adiposity of the trunk and lipomatosis, especially of the dorsocervical spine. Changes in body composition have been reported in 40-50% of HIV-infected patients. Several studies have shown that antiretroviral therapy contributes to changes in body composition and is coupled with increased dyslipidemia, insulin resistance and diabetes. Accumulation of fat over the dorsocervical spine, or buffalo has been reported in 2% to 13% of HIV-infected patients. Enlargement of adipose tissue in the dorsocervical region involves subcutaneous fat and is therefore unique to fat accumulation of the abdominal area. Guallar et al. examined dorsocervical adipose tissue after surgical removal and found that adipose tissue in this area showed substantial levels of the marker gene of brown fat, uncoupling protein 1 (UCP-1) suggesting there may be brown adipose tissue (BAT) in HIV infected individuals with lipomatosis of the dorsocervical spine. Until recently, BAT was known to be present in rodents throughout their lifetime and was thought to be present in humans only during infancy and early childhood. However, recent studies using 18F-FDG PET-CT have confirmed the presence of BAT in adults. Brown adipose tissue is known to affect whole-body metabolism and may be related to insulin sensitivity as well as susceptibility to weight gain. Using 18F-FDG PET techniques, our group has evaluated HIV-infected subjects with lipoatrophy and noted there was significantly increased glucose uptake into subcutaneous tissue which may suggest presence of BAT in HIV-infected patients. However our previous study did not specifically examine areas of BAT in the subjects. Therefore, using 18F-FDG PET-CT in addition to fat biopsies we propose to explore the presence of BAT in fat depots among HIV-infected patients with fat redistribution, focusing specifically in the cervical area. Also, as recent studies suggest down regulation of Dicer, a major component of miRNA has an important role in the differentiation and function of brown and white adipose tissue and may contribute to lipodystrophy. We will perform subcutaneous fat biopsies of the dorsocervical and abdominal fat in a subset of HIV-infected and non-HIV-infected men in order to explore further the question of down regulation of Dicer and its implication on metabolic abnormalities in this population.

Official Title
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FDG/PET Imaging for the Assessment of Brown Adipose Tissue in HIV Lipodystrophy

Conditions
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HIV Infections

Participation Criteria
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Eligibility Criteria
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Subject Selection and Enrollment: Twenty male subjects comprised of four distinct groups will be recruited for the study. Subjects will be matched by age and body-mass index. The four groups are: Group 1: HIV infected with fat redistribution (lipohypertrophy) (n=5) Group 2: HIV infected with fat redistribution (lipoatrophy) (n=5) Group 3: Healthy Controls (n=10) Inclusion Criteria for HIV+ with fat redistribution (lipohypertrophy) subjects (Group 1) Evidence of HIV infection Age ≥ 20 and ≤ 60 years of age BMI measurement between 25-29.9 kg/m2 HIV positive, on a stable HAART treatment regimen (including an NRTI) for > 12 months Evidence of significant fat redistribution rated by the investigator, including 1) significant fat atrophy of the face, arms or legs, and 2) significant increase in fat accumulation of the neck. Exclusion Criteria for HIV+ with fat redistribution (lipohypertrophy) (Group 1) Hemoglobin < 10.0 g/dL Diabetes or on medications for diabetes Abnormal thyroid function Therapy with medications such as beta blockers, alpha-blockers, sympatholytic drugs Chronic adrenergic drug use (>3 months) and benzodiazepine use. Therapy with glucocorticoids (oral and inhaled), growth hormone or other anabolic agents currently or within the past 3 months Current substance abuse, including alcohol, cocaine and/or heroin Other serious or chronic diseases New antiretroviral regimen in the past 12 months Any new serious opportunistic infection within the past 6 weeks Inclusion Criteria for HIV+ with fat redistribution (lipoatrophy) (Group 2) Evidence of HIV infection Age ≥ 20 and ≤ 60 years of age BMI measurement between 18-24 kg/m2 HIV positive, on a stable HAART treatment regimen (including an NRTI) for > 12 months No evidence of fat redistribution rated by the investigator. Exclusion Criteria for HIV+ with fat redistribution (lipoatrophy) (Group 2) Hemoglobin < 10.0 g/dL Diabetes or on medications for diabetes Abnormal thyroid function Therapy with medications such as beta blockers, alpha-blockers, sympatholytic drugs Chronic adrenergic drug use (>3 months) and benzodiazepine use. Therapy with glucocorticoids (oral and inhaled), growth hormone or other anabolic agents currently or within the past 3 months Current substance abuse, including alcohol, cocaine and/or heroin Other serious or chronic diseases New antiretroviral regimen in the past 12 months Any new serious opportunistic infection within the past 6 weeks Inclusion Criteria for Healthy Controls (Group 3) No history of HIV infection Age ≥ 20 and ≤ 60 years of age BMI measurement between 18-29.9 kg/m2 Exclusion Criteria for Healthy Controls (Group 3) Hemoglobin <10.0 g/dL. Diabetes or on medications for diabetes Abnormal thyroid function. Therapy with medications such as beta blockers, alpha-blockers, sympatholytic drugs Chronic adrenergic drug use (>3 months) and benzodiazepine use. Therapy with glucocorticoid (oral and inhaled), growth hormone or other anabolic agents currently or within the past 3 months Current substance abuse, including alcohol, cocaine and/or heroin Any history of serious or chronic diseases -

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| HIV-infection with fat redistribution (lipoatrophy)<br>Evidence of HIV infection Age ≥ 20 and ≤ 60 years of age BMI measurement between 18-24 kg/m2 HIV positive, on a stable HAART treatment regimen (including an NRTI) for > 12 months No evidence of fat redistribution rated by the investigator. | |
| Healthy controls<br>No history of HIV infection Age ≥ 20 and ≤ 60 years of age BMI measurement between 18-29.9 kg/m2 | |
| HIV-infected with fat redistribution (lipohypertrophy)<br>Evidence of HIV infection Age ≥ 20 and ≤ 60 years of age BMI measurement between 25-29.9 kg/m2 HIV positive, on a stable HAART treatment regimen (including an NRTI) for > 12 months Evidence of significant fat redistribution rated by the investigator, including 1) significant fat atrophy of the face, arms or legs, and 2) significant increase in fat accumulation of the neck. | |
| Dicer Cohort<br>30 men [HV-infected with fat redistribution (n = 10), HIV-infected without fat redistribution (n=10), and healthy controls (n= 10)] will be recruited for this group. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Brown Fat | Brown Fat will be assessed by PET FDG | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| UCP-1 | UCP-1 will be analyzed from tissue collected from a fat biopsy of dorsocervical spine fat accumulation. | Baseline | 
| Indirect Calorimetry | Indirect Calorimetry will be performed to measure resting energy expenditure | Baseline | 
| Anthropometrics | Anthropometric measurements of waist to hip ratio, leg circumference, arm circumference and neck circumference will be performed using a standardized technique. | Baseline | 
| Glucose tolerance | A baseline glucose level will be obtained and then patients will consume a 75g glucose beverage. Subjects must complete the beverage within 5-10 minutes. Subsequently, blood glucose at +30, +60, +90, and +120 minutes, insulin levels will be assessed at baseline and +120 minutes. Glucose tolerance will be calculated by insulin area under the curve in response to OGTT. | Baseline | 
| miRNAs | A baseline miRNA profile will be assessed and subsequent pathways | Baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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HIV, fat redistribution, brown adipose tissue, PET