Identifying Targets of Maladaptive Metabolic Responses in Heart Failure

Study Overview
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Brief Summary
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The purpose of the research is to help researchers understand changes in metabolism in patients that develop heart failure.

Official Title
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Identifying Targets of Maladaptive Metabolic Responses in Heart Failure

Conditions
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Heart Failure

Intervention / Treatment
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* Procedure: Collection of discarded heart tissue


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subjects are capable of giving informed consent. Age 19-89 Diagnosis of chronic heart failure Any patients scheduled for at least one of the following procedures: LVAD implantation or as recipient for cardiac transplantation. LVAD placement both as bridge to transplant and as destination therapy. LVAD explantation Valve replacement or repair Catheterization and myocardial biopsy for idiopathic cardiomyopathy Coronary artery bypass graft (CABG) Exclusion Criteria: Life expectancy less than 2 weeks Diagnosed with following co-morbidities: cancer, chronic kidney disease (GFR≤30 or serum creatinine >2.1), active infection or treatment with antibiotics or persistent infectious disease (hepatitis, HIV, etc), cirrhosis, active substance abuse and/or alcohol dependence (≥ 14 alcoholic beverages per week). Control Myocardial Tissue from Donor Hearts Inclusion Criteria Samples collected from non-failing hearts deemed unsuitable for transplantation by TransLife because of either acute recipient issues or post-harvest evidence of pathology or positive but benign serology. Normal cardiac function from medical records Exclusion Criteria: Pathological remodeling and extensive infarction with limited non-pathological regions of myocardium. Abnormal or impaired cardiac function from medical records, which are self determinants for rejection as a donor organ.

Ages Eligible for Study
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Minimum Age: 19 Years
Maximum Age: 89 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Cardiac surgery<br>Subjects will be recruited by a coordinator through electronic medical record (EMR) searches to identify those undergoing left ventricle assist device (LVAD) implantation and explantation, heart transplant, valve replacement or repair, endomyocardial biopsy during catheterization, and arterial bypass surgery. Prior to the procedure, potential subjects will be informed about the clinical study and if interested, they will be consented. | Procedure: Collection of discarded heart tissue<br>* Discarded heart tissue will be collected from patients undergoing the aforementioned cardiac surgeries.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Analyses of collected tissues | Tissue samples collected during surgery will be analyzed by way of immunoblot and qrtPCR of mRNA for metabolic enzymes on neutralized acid extracts. | Restricted to the time around surgical procedure |