Effects of Exercise Training on Vascular Endothelial Function of Patients Submitted to Coronary Artery Bypass Graft

Study Overview
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Brief Summary
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Objective: To observe the effects of exercise training on vascular endothelial function in patients undergoing coronary artery bypass graft (CABG) alone in phase III after six months of Cardiac rehabilitation programs (CRP). Methods: the investigators contacted all patients undergoing CABG alone in period of 1 year to participate in a CRP with duration of six months with three weekly sessions of 1 and half hour of the duration. All patients underwent biochemical blood tests, muscle strength testing of one repetition maximum (1-RM test) for upper and lower limbs, 6-minute walk test (6MWT), and evaluation of endothelial function (using flow-mediated vasodilation).

Detailed Description
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Introduction The cardiac rehabilitation optimizes a cardiac patient's physical, psychological, and social functioning, in addition to stabilizing, slowing, or even reversing the progression of the underlying atherosclerotic processes, thereby reducing morbidity and mortality. Patients who recently had had a myocardial infarction; or had undergone coronary artery bypass graft; or who had undergone percutaneous coronary interventions; or who are heart transplantation candidates or recipients; or have stable chronic heart failure, peripheral arterial disease with claudication, or other forms of CVD are candidates eligible for cardiac rehabilitation services ( , ). Recently, studies randomized clinical trials have reported the efficacy of the exercise training (ET) independently of format of exercise: aerobic, resistance, or combined exercise training in patients with coronary artery disease ( , ) and, others studies investigated the effects of ET on vascular endothelial function in patients with stable coronary artery disease ( ), ischemic heart disease ( ), recent myocardial infarction (4), and chronic heart failure (3, , ). ET is associated with an improvement in endothelial function, oxidative stress, and inflammation. Furthermore, the ET promotes beneficial effects on cardiovascular outcomes and decreases hospitalizations. Arterial endothelium function evaluated by flow-mediated vasodilation (FMD) is a predictor of long-term adverse cardiovascular outcomes in healthy subjects ( ). Vascular endothelial dysfunction is an independent risk factor for cardiovascular events and provides important prognostic data in addition to the more traditional risk factors ( ). Endothelial dysfunction plays an important role in patients with coronary atherosclerosis (both acute and chronic myocardial ischemia) and heart failure (3,5, ). Therefore, we aim to investigate the effect of combined exercise training on vascular endothelial function in sedentary patients submitted to coronary artery bypass graft (CABG) in late phase. Methods The protocol was approved by the local ethics committee of Associação de Caridade Santa Casa do Rio Grande Hospital, Rio Grande/RS - Brazil, under the protocol number 003/2013. This study was non-randomized clinical trial. The written informed consent was obtained from all patients before of the onset of ET. The investigators invited all consecutive patients submitted to CABG alone at Cardiologic Hospital Doctor Pedro Bertoni of the Associação de Caridade Santa Casa do Rio Grande in the period of October, 2011 until October, 2012, who resided in the city of the Rio Grande (RS/Brazil), to participate of the ET. The investigators excluded the patients that performed others cardiologic procedures with the CABG. All patients were evaluated by cardiologist, and were ranked in Functional Classification of New York Heart Association (NYHA) such class I and II. Initially, twenty-four patients accepted and start to participate of ET, but only nine patients completed the program until the end. The program of exercise training (ET) was employed for 6 consecutive months with 3 sessions per week. The training of physical activity consisted of a combination of aerobic and resistance exercises for upper and lower limbs. Each session included: (1) 5-minute warm-up, (2) 30 minutes of aerobic exercise (performed in treadmills and stationary bikes); (3) 20 minutes of resistance exercises with dumbbells for upper limbs and ankle support for lower limbs (exercises preformed in diagonals) that consisted of 6 sets of 10 resistance exercises repeated for upper limbs, and the same number of sets for lower limbs; and (4) 5-minute cool-down, totaling ninety minutes of session per day (90min/day) and, two hundred and seventy minutes per week (270min/week) ( ). Initialy, in aerobic exercises, the maximal HR to be achieved was established between 30-40% of HR at rest and, the aerobic exercise was limited by symptoms reported by patients. The initial weight of the dumbbells and ankle were established between 30-40% of one-repetition maximum test (1-RM test) and, gradually increased according to the symptoms reported by patients, without compensation of the trunk to perform the exercises, no history of muscle pain, and no muscle fatigue to complete all sessions. Heart rate (HR), respiratory rate (RR), saturation pulse of oxygen, and blood pressure were measured at the beginning, middle and end of each session. The intensity and the velocity of the exercise program were progressively increased. The baseline assessment was performed before the beginning of ET and, consisted of: blood chemistry (glycemia, insulin, glycohemoglobin, total-, high-, and low-density lipoprotein cholesterol, triglycerides); body mass index; strength of upper and lower limbs by 1-RM test of biceps brachialis and quadriceps, respectively; evaluation of functional capacity and; evaluation of vascular endothelial function. Cholesterol, triglycerides, high-density lipoproteins (HDLc) and, glucose urea were measured using LAB TEST commercial kits (Lagoa Santa, MG, Brazil) and analyzed in LAB MAX 240® (Tokyo, Japan) equipment. The low density lipoproteins (LDLc) was calculated by Friedewald's formula. Glucose levels were measured by Trinder assay (calorimetry) in the equipment LAB MAX 240® (Tokyo, Japan). The insulin was assessed by chemiluminescence method using the Immulite® DPC equipment (Diagnostic Products Corporation - DPC, Los Angeles, CA, USA). The glycosylated hemoglobin (HbA1c) was determined by enzymatic method using the equipment LAB MAX 240® (Tokyo, Japan). The 6-minutes walking test (6MWT) is used to assess the functional capacity of the individual, which is extremely important to evaluate tolerance and exercise capacities for the evaluation clinical and prognosis of patients with cardiovascular and pulmonary diseases ( , ). The 6MWT was performed on the first day in a linear corridor with 40 meters of length, and the researcher performed the test giving verbal commands to the patient, which should be done the best speed supported by him, and the researcher asked to the patient what their physical sensations. Data were collected before, at 3 minutes and at the end of the test for respiratory rate (RR), heart rate (HR), saturation pulse in portable pulse oximeter (Nonin®, model 9500, Plymouth, USA), and blood pressure systemic. Flow-mediated vasodilation (FMD) was measured to evaluating arterial endothelium-dependent vasodilation using a high-resolution vascular ultrasound (Logiq P6, GE Healtcare, GE Ultrasound Korea Ltda.) according to American Heart Association Guideline ( ) with adjustments ( , ). Settings of depth and gain imaging were kept constant throughout the study. Briefly, changing in brachial artery diameter after 60 s of reactive hyperemia, after deflation of a cuff placed around the upper arm and inflated to 50 mmHg above the systolic blood pressure during five min, was compared with a baseline measurement. To evaluate basal blood flow and flow immediately after cuff release obtained no later than 15 s after cuff deflation we used a pulsed-wave Doppler velocity signals (assessed using Doppler bean-vessel angle ≤60º). The increased diameter after a sublingual nitroglycerin spray (0.4 mg) was used as a measurement of endothelium-independent vasodilation. The vessel diameter responses to reactive hyperemia and to nitroglycerin were expressed as the percentage changing relative to diameter immediately before cuff inflation and to diameter immediately before drug administration. Measurements of brachial artery diameter were accomplished off line by two evaluators using a semiautomatic quantitative analysis system after interventions. The second evaluator performing measures was blind to first evaluator's measures. Differences larger than 0.01 mm between assessors (mean vessel diameter) repeated were repeated. All data were measured twice, and final values were present as mean. A nutritional counseling was performed, in wich the patients were instructed to avoid consumption of saturated fat and high-calorie foodstuff, to intake of dietary fiber, to maintain good hydration and to implement a fractionation of meals throughout the day, besides to receive instructions of food preparation, according to I Diretriz sobre o Consumo de Gorduras e Saúde Cardiovascular in Arquivos Brasileiros de Cardiologia of the Sociedade Brasileira de Cardiologia ( ). All tests were repeated after 6 months, and the results were compared to baseline tests. The training program was stopped at this point. The data are presented as the means ± SD. Distribution of variables was tested by Shapiro Wilk normality test. Baseline values were compared to values after 6 months of training program by use paired t-test. A value of P<0.05 was considered statistically significant.

Official Title
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Effects of Exercise Training on Vascular Endothelial Function of Patients Submitted to Coronary Artery Bypass Graft

Conditions
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Vasodilation

Intervention / Treatment
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* Other: exercise training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: resided in the city of the Rio Grande (RS/Brazil); age over 18 years-old; evaluation and medical authorization. Exclusion Criteria: the patients that performed others cardiologic procedures with the Coronary Artery Bypass Graft

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Intervention Model: Single Group Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: cardiac rehabilitation<br>We invited all consecutive patients submitted to CABG alone at Cardiologic Hospital Doctor Pedro Bertoni of the Associação de Caridade Santa Casa do Rio Grande in the period of October, 2011 until October, 2012, who resided in the city of the Rio Grande (RS/Brazil), to participate of the exercise training (ET). All patients were evaluated by cardiologist, and were ranked in Functional Classification of New York Heart Association (NYHA) such class I and II. Initially, twenty-four patients accepted and start to participate of ET, but only nine patients completed the program until the end. | Other: exercise training<br>* The program of exercise training (ET) was employed for 6 consecutive months with 3 sessions per week. The training of physical activity consisted of a combination of aerobic and resistance exercises for upper and lower limbs. Each session included: (1) 5-minute warm-up, (2) 30 minutes of aerobic exercise (performed in treadmills and stationary bikes); (3) 20 minutes of resistance exercises with dumbbells for upper limbs and ankle support for lower limbs (exercises preformed in diagonals) that consisted of 6 sets of 10 resistance exercises repeated for upper limbs, and the same number of sets for lower limbs; and (4) 5-minute cool-down, totaling ninety minutes of session per day (90min/day) and, two hundred and seventy minutes per week (270min/week).<br>* Other names: cardiac rehabilitation;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Vascular endothelial function |  | after 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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endothelial function, physical training, exercise, coronary artery bypass