Is Lymphocyte Subtype Important for Acute Pancreatitis Severity?

Study Overview
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Brief Summary
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Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease. In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis and lymphocyte subtypes with Flow-cytometry.

Detailed Description
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Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In patients with severe pancreatitis, aggressive fluid replacement, organ damage follow-up, appropriate antibiotherapy, and endoscopic sphincterotomy and radiological interventions may be of great benefit. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease. There are limited number of studies in the literature about the immune parameters in the evaluation of acute pancreatitis. In a studies, serum inflammatory markers such as IL-1, IL-6 and CD4, CD8 T lymphocyte and Treg population were evaluated.Treg cells are reported to be an independent prognostic factor in determining the severity of acute pancreatitis. In patients diagnosed with acute biliary pancreatitis, determination of the course of the disease at the time of diagnosis is extremely important for treatment and survival. In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis with lymphocyte subtypes via Flow-cytometry.

Official Title
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Can we Measure Pancreatitis Severity With Flow Cytometry?

Conditions
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Pancreatitis, Lymphocytic Infiltrate, Immune System Diseases

Intervention / Treatment
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* Diagnostic Test: Flow Cytometric analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18-80 years old Diagnosis of acute biliary pancreatitis Sign the voluntary consent form Exclusion Criteria: Being under 18 or older than 80 Not signing the voluntary consent form Pancreatitis resulting from an interventional procedure (ERCP, surgery, etc.) Pregnant women Being a history of immunodeficiency Cancer history

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Mild pancreatitis<br>Patients with mild acute biliary pancreatitis according to Atlanta classification | Diagnostic Test: Flow Cytometric analysis<br>* Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups<br>|
| Moderate Pancreatitis<br>Patients with moderate acute biliary pancreatitis according to Atlanta classification | Diagnostic Test: Flow Cytometric analysis<br>* Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups<br>|
| Severe pancreatitis<br>Patients with severe acute biliary pancreatitis according to Atlanta classification | Diagnostic Test: Flow Cytometric analysis<br>* Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups<br>|
| Control<br>Healthy volunteers | Diagnostic Test: Flow Cytometric analysis<br>* Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Lymphocyte subtypes 1 | T helper (CD4) (%) | 9 months | 
| Lymphocyte subtypes 2 | T cytotoxic (CD8) (%) | 9 months | 
| Lymphocyte subtypes 3 | Treg (CD25 and CD127) (%) | 9 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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pancreatitis, lymphocyte, Flow-cytometry