Histopathological Analysis of Temporal Artery Biopsy Following Dynamic Full-field Optical Coherence Tomography, a Comparison to Conventional Histopathological Findings in Patients With Suspected Giant Cell Arteritis (DOCTA)

Study Overview
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Brief Summary
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Giant cell arteritis (GCA) is a type of large vessel granulomatous vasculitis responsible for the inflammation of the aorta and the branches of the external carotid, notably temporal arteries. The diagnosis of GCA relies upon the identification of vasculitis following histopathological analysis of temporal artery biopsy (TAB) showing mononuclear cells infiltration, fragmentation of the internal elastic lamina as well as significant intimal hyperplasia. Apart from its lack of sensitivity, one of the weaknesses of TAB is the delay in obtaining the result due to the time required to prepare the sample for histological analysis. Pursuing the idea to improve TAB performances, our group recently demonstrated the use of full-field optical coherence tomography (FF-OCT) to visualize structural changes associated with the inflammatory processes of GCA. The present work suggests a further use of dynamic FF-OCT on TAB for a direct visualization of the mononuclear cells infiltration to ensure rapid on-site diagnosis of GCA.

Official Title
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Histopathological Analysis of Temporal Artery Biopsy Following Dynamic Full-field Optical Coherence Tomography, a Comparison to Conventional Histopathological Findings in Patients With Suspected Giant Cell Arteritis

Conditions
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Giant Cell Arteritis, Tomography, Optical Coherence

Intervention / Treatment
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* Other: Dynamic full-field optical coherence tomography analysis of temporal artery biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: patient > 50 years of age with suspected giant cell arteritis who received temporal artery biopsy between start study date and primary completion date Exclusion Criteria: inability to perform dynamic full-field optical coherence tomography observation at the moment of temporal artery biopsy

Ages Eligible for Study
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Minimum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| DOCTA cohort<br>Patients > 50 years of age with suspected giant cell arteritis requiring temporal artery biopsy in the dermatology department | Other: Dynamic full-field optical coherence tomography analysis of temporal artery biopsy<br>* Dynamic full-field optical coherence tomography analysis of temporal artery biopsy in the dermatology department before conventional histopathological analysis<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Histopathological analysis of healthy temporal artery biopsy with dynamic full-field optical coherence tomography | Provide a better understanding of the ability of dynamic full-field optical coherence tomography to identify the normal structures of a temporal artery biopsy, i.e. the tripartite architecture with a clear distinction between intima (endothelial cells), media (vascular smooth muscle cells) and adventitia, both internal and external elastic lamina, and vasa vasorum | Outcome measure is assessed 15 days following temporal artery biopsy | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Histopathological features of giant cell arteritis with dynamic full-field optical coherence tomography | Provide a better understanding of the ability of dynamic full-field optical coherence tomography to identify histopathological features of giant cell arteritis, i.e. infiltration of mononuclear cells in the three layers of the artery, fragmentation of the internal elastic lamina, intimal hyperplasia and neoangiogenesis | Outcome measure is assessed 15 days following temporal artery biopsy | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Giant Cell Arteritis, Temporal Artery Biopsy, Full-field Optical Coherence Tomography