Effects of Collagen Peptide Supplementation on Muscle and Tendon Damage and Function

Study Overview
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Brief Summary
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The aim of the study is to investigate the acute and short-term effects of collagen peptides (Collagen Peptan) on muscle and connective tissue during an eccentric overload training period. Currently, all studies investigating the effects of post-exercise collagen supplementation did this in the absence of whey proteins, which are part of the standard recommendations for post-exercise recovery. The investigators will therefore investigate whether the combined intake of whey proteins and collagen peptides ensures a better recovery of exercise performance and whether it can prevent or reduce symptoms of muscle and tendon overload during a three-week eccentric overload training period (n = 22).

Official Title
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A Randomized Double-blinded Placebo-controlled Study to Evaluate the Effect of Collagen Peptide (Collagen Peptan) Supplementation on Muscle and Tendon Damage, Repair, and Functional Capacity During Exercise Training Overload

Conditions
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Exercise, Healthy Male Subjects

Intervention / Treatment
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* Dietary Supplement: whey protein
* Dietary Supplement: whey protein + collagen peptide


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Sports participation, including general fitness training, between 2 and 5 hours max per week Body mass index between 18.5 and 25 Exclusion Criteria: Any kind of injury/pathology that is a contra-indication to perform high-intensity exercise - Intake of any medication or nutritional supplement that could impact muscle protein synthesis and/or exercise performance during the period of the study Intake of any whey protein, casein of branched-chain amino acid (BCAA) supplements from 1 month prior to the start of the study Blood donation within 3 months prior to the start of the study Smoking Current participation in another research trial Any other argument to believe that the subject is unlikely to successfully complete the full study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Placebo<br>Only whey protein will be provided | Dietary Supplement: whey protein<br>* Daily 45g of whey protein supplementation during a three week eccentric training period<br>|
| Experimental: Collagen<br>A mix of whey protein and collagen peptides will be provided | Dietary Supplement: whey protein + collagen peptide<br>* Daily 15g of whey protein and 20g collagen peptide supplementation during a three week eccentric training period<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in countermovement jump performance | Change in jump height when performing a countermovement jump, measured via a force platform | Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention) | 
| Change in maximal isometric knee-extension force of the right leg | Change in maximal isometric knee-extension force (angle = 45°) of the right leg | Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention) | 
| Change in anteroposterior thickness of the right patellar tendon | Change in anteroposterior thickness of the right patellar tendon | Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention) | 
| Change in neovascularization of the right patellar tendon | Neovascularization of the right patellar tendon scored with the Modified Ohberg Score (range 0 - 4; the higher the score, the higher the degree of neovascularization). | Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in pain perception at the muscular level (VAS scale) | Change in pain perception at the muscular level of the right leg, measured via the Visual Analogue Scale (range 0 - 10; the higher the score, the higher the pain level) | Training 1 (day 1 of training intervention) - Training 2 (day 3 of training intervention) - Training 8 (day 9 of training intervention) - Training 17 (day 19 of training intervention) - Posttest (day 22 of training intervention) | 
| Change in pain perception of the patellar tendon of the right leg (VISA-P) | Change in pain perception of the patellar tendon of the right leg, measured via the VISA-P questionnaire (range: 0 - 100; the lower the score, the higher the symptoms of patellar tendon overload) | Pretest - Training 1 (day 1 of training intervention) - Training 17 (day 21 of training intervention) - Posttest (day 22 of training intervention) | 
| Change in the blood concentration of creatine kinase | Change in blood concentration of creatine kinase (marker of muscle damage) | Pretest - Training 2 (day 3 of training intervention) - Posttest (day 22 of training intervention) | 
| Change in the blood concentration of Interleukin 6 (IL-6) | Change in blood concentration of Interleukin 6 (IL-6; marker of inflammation) | Pretest - Training 2 (day 3 of training intervention) - Posttest (day 22 of training intervention) | 
| Change in the blood concentration of N-terminal propeptide of type I procollagen (P1NP) | Change in blood concentration of N-terminal propeptide of type I procollagen (P1NP; marker of collagen synthesis) | Pretest - Training 2 (day 3 of training intervention) - Posttest (day 22 of training intervention) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Whey protein, Collagen peptide, Muscle and tendon overload training