Impact of E-cigarette Use on the Body

Study Overview
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Brief Summary
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This study examines how electronic (e)-cigarette use impacts the body, by studying both users and non-users of e-cigarettes. Early evidence indicates that e-cig users experience adverse health effects. Results of this study may help policy-makers develop standards for different types of tobacco.

Detailed Description
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PRIMARY OBJECTIVE: Examine the short-term impacts of vaping on pulmonary and respiratory outcomes. OUTLINE: Current e-cigarette users will vape their own e-cigarette device ad libitum for 30 minutes; non-users will watch a neutral video for 30 minutes. Before and after the 30 minute vaping [video] session, participants will undergo the following assessments: computer-based spirometry testing, nitric oxide breath testing, airwave oscillometry, and an assessment of endothelial function. Participants will also undergo collection of blood.

Official Title
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The Emerging Adulthood Health Project

Conditions
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Cigarette Smoking-Related Carcinoma

Intervention / Treatment
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* Procedure: Biospecimen Collection
* Procedure: Nitric Oxide Breath Test
* Procedure: Oscillometry
* Procedure: Spirometry
* Other: Survey Administration
* Procedure: Endothelial function


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 75 e-cig users and 75 never-users No self-reported diagnosis of lung disease (e.g., coronavirus disease-2019 [COVID-19], pneumonia, cystic fibrosis) No history of cardiac event or distress Not currently breastfeeding, pregnant, or planning to become pregnant (e-cig users only; will be confirmed via pregnancy test)

Ages Eligible for Study
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Minimum Age: 15 Years
Maximum Age: 25 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Prevention (survey, breathe tests, biospecimen collection)<br>Participants undergo computer-based spirometry testing, nitric oxide breath testing, airwave oscillometry, and an assessment of endothelial function. Participants will undergo collection of blood. Current e-cigarette users also vape their own e-cigarette device for 30 minutes. | Procedure: Biospecimen Collection<br>* Undergo collection of blood<br>Procedure: Nitric Oxide Breath Test<br>* Undergo nitric oxide breath testing<br>Procedure: Oscillometry<br>* Undergo airwave oscillometry<br>Procedure: Spirometry<br>* Undergo spirometry<br>Other: Survey Administration<br>* Complete survey<br>Procedure: Endothelial function<br>* Endothelial function will be assessed with the EndoPAT device, which measures changes in pressure from sensors placed on the fingertip<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Airway inflammation as assessed by exhaled nitric oxide | Exhaled nitric oxide will be measured in parts per billion (ppb) | Procedure will last approximately 3 minutes; the procedure will occur twice during the laboratory session (separated by approximately 60 minutes) | 
| Airway reactivity as assessed by airwave oscillometry | Airway reactivity will be measured via Tremoflo, which is a well validated measurement of airway reactivity and provides curves of Resistance (R) and Reactance (X) | Procedure will last approximately 3 minutes; the procedure will occur twice during the laboratory session (separated by approximately 60 minutes) | 
| Respiratory functioning as assessed by a mobile Spirometry device | Spirometry will collect daily data from participants on the relationship between their forced expiratory volume and forced vital capacity (measured as FEV1/FVC). | Procedure will last approximately 3 minutes; the procedure will occur twice during the laboratory session (separated by approximately 60 minutes) | 
| Endothelial function | Endothelial function will be assessed with the EndoPAT device (unit of measurement = LnRHI). | Procedure will last approximately 20 minutes; the procedure will occur twice during the laboratory session (separated by approximately 90 minutes) |