Advantages of Autologous Platelet-Rich Fibrin Membrane on Growth Factor Levels and Periodontal Healing

Study Overview
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Brief Summary
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Platelet rich fibrin is a second generation platelet concentration which contains various growth factors. The aim of this study is to evaluate the clinical and biochemical efficacy of PRF in subjects of chronic periodontitis in the operation of conventional periodontal flap.

Detailed Description
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PRF was developed in 2001 by Choukroun in France, it was the second generation fibrin adhesive, and was used to accelerate the soft and hard tissue healing. Many studies have shown beneficial effects of PRF on osteoconductive filler material in sinus lift operations, treatment of periodontal sulcus defect and treatment of furcation defects. Also PRF contains many growth factors. PRF accelerates periodontal regeneration through the gradually excreted growth factors and contributes to the bone regeneration. In this study, the investigators evaluated the effect of PRF on growth factors and its contribution to periodontal regeneration in subjects with chronic periodontitis.

Official Title
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Investigation of the Contributions of Platelet Rich Fibrin Combined With Open Flap Debridement on Periodontal Healing

Conditions
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Chronic Periodontitis

Intervention / Treatment
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* Device: Platelet rich fibrine
* Device: Open Flap Debridement


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients with CP who had pocket depth ≤ 5 mm and at least two area (grouped as Control and PRF) with horizontal bone loss after initial treatment Exclusion Criteria: The subjects excluded in the study had history of systemic disease, were smokers, had allergy to any drug, had need for prophylaxis of antibiotic and had taken antibiotics at least 6 months
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Platelet Rich Fibrine<br>Platelet Rich Fibrine was applied with open flap debridement in test group. | Device: Platelet rich fibrine<br>* Platelet rich fibrin increases the growth factors. So it was applied for treatment of periodontal bone loss.<br>|
| Active Comparator: Open Flap Debridement<br>Platelet Rich Fibrin was not applied to control groups. Only open flap debridement was applied to control groups. | Device: Open Flap Debridement<br>* Only open flap debridement treatment the bone loss limitedly<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Level of Growth Factor | Change from baseline growth factors levels of GCF at 6 weeks were measured by periopaper | 6 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Alteration of Clinical Attachment Level | Change from baseline Clinical Attachment Levels at 9 months were measured with periodontal probe. | 9 month | 
| Alteration of Periodontal Pocket Depth | Change from baseline Pocket Depth at 9 months were measured with periodontal probe. | 9 month |