Studying Genes Using Cord Blood and Placenta Samples From Relatively Healthy Newborns and Samples From Younger Patients With Wilms Tumor

Study Overview
=================
Brief Summary
-----------------
RATIONALE: Studying samples of blood and tissue from newborns and from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies cord blood and placenta tissue from newborns, and tumor tissue samples from patients with Wilms tumor.

Detailed Description
-----------------
OBJECTIVES: What is the frequency of loss of imprinting at birth (in the cord blood and placenta) in a relatively healthy birth cohort? Does deoxyribonucleic acid (DNA) methylation levels at imprinting genes have a direct association to the gene expression? OUTLINE: Archived tumor tissue, cord blood, and placenta samples are analyzed for DNA methylation, single nucleotide polymorphism, and gene expression by polymerase chain reaction (PCR), pyrosequencing, and quantitative real-time PCR. Information regarding gender and age of the samples are also collected, if possible.

Official Title
-----------------
Investigating the Frequency of Loss of Imprinting Across a Birth Cohort and the Link DNA Methylation Plays

Conditions
-----------------
Kidney Cancer

Intervention / Treatment
-----------------
* Genetic: DNA methylation analysis
* Genetic: RNA analysis
* Genetic: allele-specific oligonucleotide real-time quantitative polymerase chain reaction
* Genetic: gene expression analysis
* Genetic: nucleic acid sequencing
* Genetic: polymorphism analysis
* Other: laboratory biomarker analysis


Participation Criteria
=================
Eligibility Criteria
-----------------
DISEASE CHARACTERISTICS: Wilms tumor tissue samples from Caucasians (fresh or frozen), or DNA and ribonucleic acid (RNA) samples already isolated from patients registered on Children's Oncology Group Wilms tumor protocols Normal/control blood samples from matched individuals Cord blood/ placenta samples from the Michels lab Epigenetic Birth Cohort PATIENT CHARACTERISTICS: Not specified PRIOR CONCURRENT THERAPY: Not specified

Ages Eligible for Study
-----------------
Maximum Age: 21 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Intervention/Treatment |
| --- |
|Genetic: DNA methylation analysis|nan|
|Genetic: RNA analysis|nan|
|Genetic: allele-specific oligonucleotide real-time quantitative polymerase chain reaction|nan|
|Genetic: gene expression analysis|nan|
|Genetic: nucleic acid sequencing|nan|
|Genetic: polymorphism analysis|nan|
|Other: laboratory biomarker analysis|nan|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Frequency of loss of imprinting at birth |  |  | 
| Association between methylation levels and gene expression |  |  | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
Wilms tumor and other childhood kidney tumors