Pizza Meal Challenge and Immunometabolism (PIMETA)

Study Overview
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Brief Summary
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High salt intake is a major risk factor for cardiovascular events, including stroke, heart disease and kidney failure. Pizza is a popular salty food. In this exploratory pilot study, the effects of a pizza meal challenge on immunometabolism in healthy subjects will be tested.

Official Title
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Effects of a Pizza Meal Challenge on the Immunometabolism of Monocytes and T Cells

Conditions
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Healthy

Intervention / Treatment
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* Other: Pizza meal challenge


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Healthy men and women Exclusion Criteria: Severe, manifest illnesses in need of treatment Pathological lab results Inability to understand significance and scope of the study

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Pizza meal challenge<br>Vegetarian pizza containing 10 grams of salt | Other: Pizza meal challenge<br>* Vegetarian pizza containing 10 grams of salt<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oxygen consumption rate of immune cells | OCR (pmol oxygen/min/1000 cells) measured by Seahorse | 3 hours after the pizza compared to baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oxygen consumption rate of immune cells | OCR (pmol oxygen/min/1000 cells) measured by Seahorse | 3 and 8 hours after the pizza adjusted for plasma sodium concentration compared to baseline | 
| Plasma sodium | Plasma sodium concentration (mmol/l) | 3 and 8 hours after the pizza compared to baseline | 
| Mitochondrial membrane potential of immune cells | Mitochondrial membrane potential (Mean Fluorescence Intensity of TMRE) measured by flow cytometry | 3 and 8 hours after the pizza compared to baseline | 
| Extracellular acidification rate of immune cells | ECAR (mpH/min/1000 cells) measured by Seahorse | 3 and 8 hours after the pizza compared to baseline | 
| Interleukin 17 release after monocyte and T cell co-culture | IL-17 concentration (pg/ml) measured by ELISA | Cell isolation at baseline and 3 hours after the pizza followed by 72 hours of co-culture | 
| Interferon-gamma release after monocyte and T cell co-culture | INF-g concentration (pg/ml) measured by ELISA | Cell isolation at baseline and 3 hours after the pizza followed by 72 hours of co-culture | 
| Adenosine triphophate production of immune cells | Percent change of luminescence (%) measured by Luminescence Assay | 3 and 8 hours after the pizza compared to baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Salt challenge, Immune cell metabolism, Oxygen consumption rate, Extracellular acidification rate, Cytokine production