Salivary Bacterial Associations to Short Term Discontinuation of Self-performed Oral Hygiene

Study Overview
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Brief Summary
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The purpose of the present investigation is to record and compare bacterial compositions in supragingival plaque samples and saliva samples in subjects discontinuing regular oral hygiene for 10 days. The hypothesis is that the composition of the salivary microbiota might reflect local bacterial alterations in relation to discontinuation of oral hygiene.

Detailed Description
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35 orally healthy individuals will be enrolled in this study, in which they will discontinue regular oral hygiene procedures for a period of 10 days. Clinical registrations and collection of microbiological samples (supragingival and saliva) will be performed at baseline and 4, 7 and 10 days after discontinuation of regular oral hygiene, and will be repeated 14 days after regular oral hygiene has been resumed.

Official Title
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Does Short Term Discontinuation of Self-performed Oral Hygiene Procedures Influence the Composition of the Salivary Microbiota?

Conditions
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Mouth Diseases

Intervention / Treatment
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* Procedure: Oral hygiene discontinuation group


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Oral and systemically healthy individuals Exclusion Criteria: Periodontitis Dental caries Systemic diseases or medications with known associations to oral health Antibiotics within the latest 6 month

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Oral hygiene discontinuation group<br>The intervention of the present study is discontinuation of regular oral hygiene procedures for a period of 10 days. Discontinuation of oral hygiene will allow undisturbed biofilm formation (supragingival dental plaque) along the gingival margin of the teeth. After 10 days all individuals will resume regular oral hygiene. Clinical registrations and collection of microbiological samples (supragingival and saliva) will be performed at baseline and 4, 7 and 10 days after discontinuation of regular oral hygiene, and will be repeated 14 days after regular oral hygiene has been resumed. | Procedure: Oral hygiene discontinuation group<br>* Discontinuation of regular oral hygiene for 10 days<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Comparison of changes in α-diversity in saliva samples before and after discontinuation of regular oral care | Comparison of α-diversity in saliva samples before and after discontinuation of regular oral hygiene | 10 days of discontinuation of regular oral care and 14 days after resuming regular oral care | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Comparison of species specific changes in saliva samples before and after discontinuation of regular oral care | Comparison of relative abundance of bacterial species in saliva samples at baseline to samples collected 10 days after discontinuation of regular oral hygiene and to samples collected 14 days after oral hygiene procedures has been resumed | 10 days of discontinuation of regular oral care and 14 days after resuming regular oral care | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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biofilm, saliva, bacteria, oral hygiene