Pilot of Abatacept-based Immunosuppression for Prevention of Acute GvHD During Unrelated Donor HCT

Study Overview
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Brief Summary
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The primary objective of the study is to determine the safety and tolerability when adding abatacept to acute Graft versus Host Disease in transplants for malignant diseases using unrelated donor bone marrow or peripheral blood stem cell grafts.

Detailed Description
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Acute Graft versus Host Disease (aGvHD) is the most deadly complication facing children who have allogeneic hematopoietic stem cell transplant (HSCT). aGvHD occurs, in large part, because the T cells in the bone marrow graft do not accept the presence of the transplant recipient's cells, and mount a severe, debilitating, and often deadly attack against the recipient, striking the skin, the liver, and the gastrointestinal track, most prominently. For patients receiving bone marrow from an unrelated donor, the rate of aGvHD can reach as high as 80%, with up to half of patients dying from this complication. These serious outcomes occur despite our best efforts at aGvHD prevention. Given the lack of success in preventing aGvHD with current therapies, novel therapies to prevent this disease are desperately needed. In this study, we plan to test a novel drug to prevent aGvHD. This drug, known as abatacept, specifically blocks the activation pathway critical to T cell function known as T cell costimulation. In particular, it blocks the CD28-mediated costimulation pathway that is critical for optimal T cell activation and proliferation. My research group has done extensive pre-clinical work with this compound. Our work has demonstrated its efficacy in inducing immune tolerance after transplantation in both mouse models and primate models. In addition, patient trials have demonstrated that blocking CD28-directed T cell costimulation can prevent T cell-mediated diseases, including rheumatoid arthritis and psoriasis, and can improve solid organ transplant acceptance. Abatacept is currently FDA approved for use in rheumatoid arthritis. Given this drug's safety and efficacy profile, we have been granted an IND-exemption from the FDA for the inclusion of abatacept in a GvHD-prevention strategy. This is a safety and tolerability study of the addition of abatacept to a GvHD-prophylaxis regimen. Thus, the primary objective of the study is to determine the safety and tolerability of the addition of abatacept to aGvHD prophylaxis in transplants for malignant hematologic disease using unrelated donor bone marrow or peripheral blood stem cell grafts. Three secondary objectives will also be addressed: We will estimate the incidence and severity of aGvHD in patients receiving the abatacept-based protocol. We will determine the immune phenotype of donor cells in patients receiving abatacept. We will determine the ability of donor T-cells in patients receiving abatacept to respond to both polyclonal and recipient-specific immune stimulation. These secondary objectives will allow us to determine the impact of abatacept-containing GvHD prevention on both T cell alloreactivity and on T cell-mediated protective immunity. This study is for patients older than 12 who have been diagnosed with high-risk leukemia and for whom an unrelated bone marrow transplant is planned. We plan to enroll 10 patients on the study, over a 1-year period from the opening of the trial. Of these ten patients, at least five will be pediatric patients; the other five may be from adult patients taken care of by Winship Cancer Center physicians. All clinical study coordination and biologic studies will be performed by CHOA personnel. Participants will receive one of two standard myeloablative conditioning regimens for their stem cell transplant, and will receive an aGvHD prophylaxis regimen including cyclosporine, methotrexate, and abatacept. They will have immunologic analysis for 1 year after transplant and clinical analysis for 3 years after transplant.

Official Title
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Safety and Tolerability Trial of Abatacept-based Immunosuppression for Prevention of Acute GvHD During Unrelated Donor Hematopoietic Stem Cell Transplant

Conditions
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AML, ALL, Undifferentiated Leukemia, Biphenotypic Leukemia, Refractory Anemia, Refractory Anemia With Ringed Sideroblasts, Refractory Cytopenia With Multilineage Dysplasia, Ref. Cytopenia w Multilineage Dysplasia & Ringed Sideroblasts, Refractory Anemia With Excess Blasts-1 (5-10% Blasts), Refractory Anemia With Excess Blasts-2 (10-20% Blasts), Myelodysplastic Syndrome, Unclassified, MDS Associated With Isolated Del (5q)

Intervention / Treatment
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* Drug: Abatacept


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients with AML, with or without a history of myelodysplastic syndrome in one of the following categories. (a) Patients in first complete remission with high-risk features Patients with ALL, in either of the following categories: In 2nd or greater complete remission (complete remission is defined as > 5% blasts in marrow) Delayed 1st CR-Failure to achieve complete remission after a single round of induction therapy Patients with undifferentiated or biphenotypic leukemia in 1st or greater complete remission. Patients with Myelodysplastic Syndrome(s) with an IPSS score of >1.5 and <10% blasts in the bone marrow at the time of transplant. These conditions will include: Refractory anemia Refractory anemia with ringed sideroblasts Refractory cytopenia with multilineage dysplasia Refractory cytopenia with multilineage dysplasia and ringed sideroblasts Refractory anemia with excess blasts-1 (5-10% blasts) Refractory anemia with excess blasts-2 (10-20% blasts) Myelodysplastic syndrome, unclassified MDS associated with isolated del (5q) Patients diagnosed with AML in CR1 after an initial diagnosis of MDS. Age 12 years or older. No prior allogeneic transplant Karnofsky performance score or Lansky Play-Performance of at least 80. Signed informed consent for adults and for minors the provision of pediatric assent and parental permission. Exclusion Criteria: Age <12 years old. Patients requiring >2 courses of induction chemotherapy to achieve remission status. HIV infection Tuberculosis Infection Chronic Obstructive Pulmonary Disease Pregnancy (positive serum b-HCG) or breastfeeding Creatinine clearance or nuclear medicine GFR of < 50 mL/min Cardiac ejection fraction < 50% bilirubin > 2 × upper limit of normal or ALT > 4 × upper limit of normal or unresolved veno-occlusive disease. Pulmonary disease with FVC, FEV1 or DLCO parameters <45% predicted (corrected for hemoglobin) or O2 saturation <92% on room air. Karnofsky performance score or Lansky Play-Performance Scale <80 Uncontrolled viral, bacterial, or fungal infection at the time of study enrollment Availability of a willing and fully MHC-matched related donor. Positive cytotoxic recipient-donor cross-match or positive HLA antibody screen against donor-disparate antigens. Any active infection. Unable to obtain informed consent.

Ages Eligible for Study
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Minimum Age: 12 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Abatacept<br>Participants will receive one of two standard myeloablative conditioning regimens for their stem cell transplant, and will receive an aGvHD prophylaxis regimen including cyclosporine, methotrexate, and abatacept. | Drug: Abatacept<br>* Participants will receive one of two standard myeloablative conditioning regimens for their stem cell transplant, and will receive an aGvHD prophylaxis regimen including cyclosporine, methotrexate, and abatacept.<br>* Other names: orencia;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Percentage of Participants With Grade III-IV Acute GVHD by Day 100. | Grade III-IV Acute GVHD by Day 100. The incidence of Gr III-IV acute GVHD was measured by the modified Glucksburg scale. | Day 100 post-transplant | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Percentage of Participants With Grades III-IV Acute GVHD at 2 Years | The rates of Grades III-IV acute GVHD were measured at 2 years according to standard Glucksberg criteria, which was 10%. | 2 years after transplant | 
| Hematologic and Immunologic Reconstitution | Flow cytometric analysis of CD4 t-cell t-cell reconstitution was performed at day +100 post-transplant. | Day +100 post-transplant | 
| Protective Immunity | Percent of CMV virus binding CD8+ t-cells at day +365 post-transplant | Day +365 post-transplant | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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cancer, leukemia, transplant, bone marrow