Molecular Response After Concurrent Exercise With Low Glycogen

Study Overview
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Brief Summary
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This is a randomized, counterbalanced crossover design, in which 14 male subjects will complete two experimental trials. Each trial will be separated by a minimum of at least 12 days (range: 12-30 days). The study aims to investigate the effects of low glycogen availability on skeletal muscle molecular response after a concurrent exercise day.

Detailed Description
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Two experimental exercise trials; a low carbohydrate (= LOW) and a high carbohydrate (= HIGH) trial. During both experimental days subjects will complete an continues submaximal cycling bout in the morning followed by a resistance exercise session in the early afternoon. During the LOW trial subjects will receive a low carbohydrate mixed meal, during the HIGH trial subjects will be provided with a high carbohydrate meal. In addition, on both experimental days subjects will receive adequate protein beverages. Lastly, protein will be provided post resistance exercise.

Official Title
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The Effects of Glycogen Availability on the Skeletal Muscle Molecular Response After Concurrent Exercise

Conditions
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Healthy

Intervention / Treatment
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* Other: Standard meal containing low or high carbohydrate content


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male gender between 18 and 30 years of age BMI between 18.5 - 25 kg/m2 Participating in regular exercise training (at least 1 exercise session, and a maximum of 3 exercise session per week) Used to different types of exercise (endurance, resistance and/or concurrent) Willing to give blood samples Willing to give muscle biopsies No use of antibiotics during the study or in the past month No drug use Suitable veins for blood withdrawal Registered by a general-practitioner Consumption of alcohol beverages is less than 21 per week No blood donor during the study No vegetarian and willing to eat meat during the experimental days Able to be present and participate at both test days and pre testing moments Not employed, or intern, or working on thesis at the department of Human Nutrition at Wageningen University Not participating in another scientific study (except EetMeetWeet) Able to participate during the experimental days en pre tests Able to speak Dutch Exclusion Criteria: Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, gluten intolerance) Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate) Use of antithrombotic therapy (marcoumar, sintromitis). Diagnosed diabetes mellitus type 1 or 2 (Chronic) injuries of the locomotor system that can interfere with the intervention

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Low carbohydrate<br>Subjects will eat a low carbohydrate diet | Other: Standard meal containing low or high carbohydrate content<br> <br> |
| Experimental: High carbohydrate<br>Subjects will eat a high carbohydrate diet | Other: Standard meal containing low or high carbohydrate content<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Proteins involved in hypertrophy |  | 10 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Concurrent, muscle, glycogen, molecular