Impact of the Blood Culture Technique on the Diagnosis of Infective Endocarditis

Study Overview
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Brief Summary
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To evaluate the performance of a single high volume blood culture sampling strategy versus the actually used multiple sampling strategy for the diagnosis and categorization of infective endocarditis according to the Duke-Li classification in a Population of adults suspected of infective endocarditis.

Official Title
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Impact of the Blood Culture Technique on the Diagnosis of Infectious Diseases: Case of Infective Endocarditis.

Conditions
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Bacteremia, Infective Endocarditis

Intervention / Treatment
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* Procedure: Blood Culture


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients older than 18 years Suspected endocarditis: Patients with Duke-Li-ESC 2015 classification as a major morphological criterion or at least two minor criteria, other than a microbiological criterion, will be considered suspicious of infectious endocarditis. Not objecting to their inclusion in the study after delivery and explanation of the information form. Absence of microbiological documentation sought or available at the time of inclusion (a patient having already had negative blood cultures or being identified during the screening can be included). Exclusion Criteria: Antibiotherapy adapted to a situation of endocarditis, introduced more than 24 hours or stopped for less than 7 days in case of therapeutic window. Any previous antibiotic therapy in the 7 days preceding the inclusion leading to an improvement in the clinical symptomatology. State of consciousness not allowing loyal information.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| one both experimental and control arm<br>Each patient experiences two methods of blood cultures. | Procedure: Blood Culture<br>* For each patient, one single high volume blood culture (3 aerobic and 3 anaerobic of 8 to 10 mL each, numbered), and then 2 samples of 16 to 20 mL (one aerobic bottle and one anaerobic for each sample).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Diagnosis of infective endocarditis: confirmed, possible or excluded | Applying the Classification of Duke-Li according to the modified diagnostic criteria of the European Society of Cardiology Recommendations 2015. | At the end of the hospitalization, or at the time of death if occurred during hospitalization; i.e. an average of 6 weeks after the inclusion | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Diagnostic performance of the single high volume blood culture for the diagnosis of infective endocarditis | according to the level of suspicion of infective endocarditis, the type of microorganism involved, the underlying cardiopathy. | At the end of the hospitalization, or at the time of death if occurred during hospitalization; i.e. an average of 6 weeks after the inclusion | 
| Measuring the nursing time required for both sampling methods. | Time for blood culture setting, processing and sending | At T0, i.e. at the inclusion of the patient | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Diagnosis, Blood Culture