Mechanisms of Endothelial Dysfunction in Type 2 Diabetes

Study Overview
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Brief Summary
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Endothelial dysfunction of conduit arteries plays an important role in the development of cardiovascular complications associated with type 2 diabetes. In order to propose targeted therapeutic approaches, this study aim to determine the mechanisms involved in endothelial dysfunction of conduit arteries in these patients.

Official Title
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Determination of the Mechanisms Involved in Conduit Artery Endothelial Dysfunction in Type 2 Diabetes

Conditions
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Diabetes Mellitus, Type 2

Intervention / Treatment
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* Biological: Blood samples


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Type 2 diabetic patients or control subjects or healthy volunteers Exclusion Criteria: Macroangiopathy Insulin treatment Chronic kidney disease (eGFR<60 ml/min/m²) Hyperlipidemia (total cholesterol>2.5 g/l) Smoking habit > 5 cigarettes/day Pregnancy

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Patient comparative approach<br>Assessing the availability of biological markers (NO, EETs, ET-1 and ROS) during endothelial stimulation using blood samples. | Biological: Blood samples<br>* Blood samples will be performed at baseline and during endothelial stimulation by hand skin heating to quantify metabolites of NO, EETs, ET-1 and ROS<br>|
| Experimental: Control subjects comparative approach<br>Assessing the availability of biological markers (NO, EETs, ET-1 and ROS) during endothelial stimulation using blood samples. | Biological: Blood samples<br>* Blood samples will be performed at baseline and during endothelial stimulation by hand skin heating to quantify metabolites of NO, EETs, ET-1 and ROS<br>|
| Experimental: Healthy volunteers metabolic approach<br>Assessing the availability of biological markers (NO, EETs, ET-1 and ROS) during endothelial stimulation using blood samples during hyperglycemia or hyperinsulinemia. | Biological: Blood samples<br>* Blood samples will be performed at baseline and during endothelial stimulation by hand skin heating to quantify metabolites of NO, EETs, ET-1 and ROS<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change from Baseline in EET concentration during endothelial stimulation |  | 30 min from the beginning of hand skin heating | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change from Baseline in NO concentration during endothelial stimulation |  | 30 min from the beginning of hand skin heating | 
| Change from Baseline in ET-1 concentration during endothelial stimulation |  | 30 min from the beginning of hand skin heating | 
| Change from Baseline in ROS concentration during endothelial stimulation |  | 30 min from the beginning of hand skin heating |