Multisystem Features of Malignant Hyperthermia or Rhabdomyolysis Related to RYR1 Variants

Study Overview
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Brief Summary
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Rationale: Malignant hyperthermia and rhabdomyolysis are phenotypes that have long been considered to occur only in response to external stimuli (trigger anaesthesia and physical exhaustion) show several features of a continuous disease manifestation. Previous studies showed prolonged bleeding time after injury, selective immunological advantages, axial muscle weakness and several social difficulties. A detailed study of the neuromuscular and multisystem features of patients with RYR1-related malignant hyperthermia or rhabdomyolysis is needed to provide clarification about the continuous and multisystem disease manifestations in these patients. Objective: Primary Objective: There are three primary objectives in this study. To investigate the neuromuscular involvement of RYR1 related MH and rhabdomyolysis. To investigate the immunological changes in subjects with RYR1 related MH and rhabdomyolysis. To identify multisystem features of RYR1 related MH and rhabdomyolysis. There are no secondary objectives. Study design: The design of the study will be a clinical, open, observational study. The study consists of three parts; a clinical, imaging and immunological part. Study population: Patients with a history of malignant hyperthermia susceptibility (MHS), and/or a history of rhabdomyolysis related to a variant in RYR1. Intervention (if applicable): Not applicable. Main study parameters/endpoints: The study consists of three parts. Each part has it's own main study parameters Clinical part: the results of the questionnaire study compared to standardizes normal values and the results of the comprehensive clinical assessment. Imaging part: fatty infiltration and hypertrophy of proximal and axial muscles. Immunological part: circulating and leukocyte released anti- and pro-inflammatory cytokine levels compared to healthy age and sex matched controls.

Official Title
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The Neuromuscular and Multisystem Features of RYR1-related Malignant Hyperthermia and Rhabdomyolysis - a Study Protocol

Conditions
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Malignant Hyperthermia, Rhabdomyolysis, RYR1 Variant

Intervention / Treatment
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* Other: Immunological challenge


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: A history of malignant hyperthermia susceptibility (MHS), confirmed by diagnostic RYR1 variant or IVCT, related to a RYR1 variant and/or a history of rhabdomyolysis related to a variant in RYR1 Minimum age 18 years old. Exclusion Criteria: Patients diagnosed with a neuromuscular disease resulting in muscle weakness (apart from RYR1 related rhabdomyolysis and malignant hyperthermia). Patients with symptoms of angina pectoris. Patients with contra-indications for MRI-scan are excluded. Contra-indications for MRI-scan include metallic implants (vascular clips, foreign bodies like metallic splinters in the eye, coronary and peripheral artery stents, prosthetic heart valves, pacemakers and ICD's, cochlear implants, breast tissue expanders and some other electronic implants or devices and known claustrophobia. Current malignancy Pregnancy or lactating Other health issues whereby patients are not able to fulfil the study protocol No written informed consent by the patient Exclusion criteria for immunological part of the study: Diabetes mellitus Patients currently using medicine affecting the immune system. Patients with a compromised immunity (e.g. HIV)• Patients with a history of auto-immune disease (e.g. SLE, psoriasis, IBD) Use of statins the past year Use of systemic corticosteroids during more than two weeks in the past 5 years Previous treatment with chemotherapy and/or radiation therapy Age > 65 years old. Exclusion criteria for healthy controls History of malignant hyperthermia Variant in RYR1 A RYR1 related myopathy History of heat stroke History of rhabdomyolysis Family history of malignant hyperthermia or a RYR1 variant Family history of inexplicable perioperative dead Family history of a RYR1 related myopathy

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| RYR1 related malignant hyperthermia/rhabdomyolysis<br> | Other: Immunological challenge<br>* In vitro immunological study of subjects with RYR1 related MH/rhabdomyolysis. Studying of circulating cytokines and cytokines production after in vitro stimulation<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To investigate the neuromuscular involvement of RYR1 related MH and rhabdomyolysis | All subjects will have a total body muscle MRI and muscle ultra sound once | Directly after inclusion, a muscle MRI's and ultra sounds will be made of all subjects. | 
| To investigate the multisystem features of RYR1 related MH and rhabdomyolysis | All subject will fulfil a questionnaire study, it will focus on pain, activity and psychological aspects. | Directly after inclusion, subjects will fulfil a questionnaire study. | 
| To investigate the multisystem features of RYR1 related MH and rhabdomyolysis | All subject will fulfil a questionnaire study, it will focus on pain, activity and psychological aspects. | Six months after inclusion, subjects will fulfil a questionnaire study. | 
| To investigate the multisystem features of RYR1 related MH and rhabdomyolysis | All subject will fulfil a questionnaire study, it will focus on pain, activity and psychological aspects. | Twelve months after inclusion, subjects will fulfil a questionnaire study. | 
| To investigate the immunological changes in subjects with RYR1 related MH and rhabdomyolysis. | Peripheral mononuclear blood cells will be isolated and in vitro challenged using pathogens and stimulatia. The results of this part of the study will be compared to healthy controls. | Directly after inclusion blood samples will be taken for immunological studies. |