From Cytotrophoblast to Syncytiotrophoblast

Study Overview
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Brief Summary
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A deeper understanding of the syncytiotrophoblast development processes and its properties may allow a better understanding of the placenta pathological process for the development of treatment models for the relevant pregnancy complications.The aim of this study is to compare telomere length, aggregate formation, and senescence markers in placentas from complicated pregnancy with placentas from normal pregnancy.

Detailed Description
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Protocol summary No. 2 for research 0202-14 of date: 22/11/2014. Rationale for the research A deeper understanding of the syncytiotrophoblast development processes and its properties may allow a better understanding of the placenta pathological process for the development of treatment models for the relevant pregnancy complications. The aim of this study is to compare telomere length, aggregate formation, and senescence markers in the syncytiotrophoblast of placentas from complicated pregnancy with placentas from normal pregnancy. Telomere lenght and aggregate formation will be assessed by the fluorescent intensity of telomere FISH. Senescence will be assessed by immunohistochemistry for the markers - SAHF, cyclin dependent kinase inhibitors p16/p21, HLAX, ERVWE1, SA-B-gal. The study groups A retrospective arm - paraffin blocks which were collected over the years from placentas from complicated pregnancy ( intrauterine growth restriction, preeclampsia , placenta accrete) will be compared to blocks of placentas from normal pregnancies. The prospective arm - placental from complicated pregnancy ( intrauterine growth restriction, preeclampsia , placenta accrete) . Will be compared to placentas of normal pregnancies. A laboratory model based on human placenta cell cultures

Official Title
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From Cytotrophoblast to Syncytiotrophoblast - Telomere Length, Aggregate Formation and Senescence in Placenta From Complicated Pregnancies Compare to Placenta From Normal Pregnancies.

Conditions
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Syncytiotrophoblast

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: placentas from normal and complicated pregnancy Exclusion Criteria: women consent

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| normal pregnancy<br>placental from normal pregnancy | |
| complacted pregnancy<br>placental from complicated pregnancy ( intrauterine growth restriction, preeclampsia , placenta accrete | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Length of telomere and senescence markers amount in the placentas. |  | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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cytotrophoblast, syncytiotrophoblast, placentas, intrauterine growth restriction, preeclampsia, placenta accrete