The Acute Effects of Commercially Available Drinks on the Endothelial Function of Humans Following a High-fat Meal

Study Overview
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Brief Summary
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High fat diets are associated with impaired endothelial function and increased cardiovascular disease risk amongst our population. These negative effects are likely caused by triglyceride induced suppression of nitric oxide, which is produced from the endothelium, and/or an increase in oxidative stress. Interestingly, previous studies have found that some beverages that are high in polyphenols and antioxidants may suppress the impairment in endothelial function observed following high fat meals/diets. Typically, these studies have investigated the ingestion of red wine, orange juice or green tea on outcome measures (typically flow mediated dilatation (FMD) of the brachial artery). Despite this previous research, no study has compared the effects of different beverages on endothelial outcomes following a high-fat meal within the same participants.

Official Title
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Pilot Study: The Acute Effects of Commercially Available Drinks on the Endothelial Function of Humans Following a High-fat Meal

Conditions
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Endothelial Dysfunction

Intervention / Treatment
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* Other: Water
* Other: Red Wine
* Other: Green Tea
* Other: Orange Juice


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Females to be postmenopausal and not on hormone replacement therapy 25 > BMI < 35 Sedentary to light activity lifestyle. Exclusion Criteria: History of cardiovascular disease (Hypertension, Atherosclerosis, endothelial issues etc). History of diabetes (Type 1 or type 2) Vegan or with food allergies or intolerances. Taking any antioxidants over the counter (Vit C, E or polyphenol supplements) Taking prescription medication for hypertension.

Ages Eligible for Study
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Minimum Age: 50 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Water + Meal<br>250 ml Water and a high calorie, high fat meal (>900 kcal, 50g fat) | Other: Water<br>* 250 ml<br>|
| Experimental: Red Wine + Meal<br>250 ml Red Wine and a high calorie, high fat meal (>900 kcal, 50g fat) | Other: Red Wine<br>* 250 ml<br>|
| Experimental: Green Tea + Meal<br>250 ml Green Tea and a high calorie, high fat meal (>900 kcal, 50g fat) | Other: Green Tea<br>* 250 ml<br>|
| Experimental: Orange Juice + Meal<br>250 ml Orange Juice and a high calorie, high fat meal (>900 kcal, 50g fat) | Other: Orange Juice<br>* 250 ml<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Endothelial (dys)function | Assessed via flow mediated dilatation | 2 hours post-prandial | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma Nitrite |  | 2 hours post-prandial | 
| Plasma antioxidant capacity | Plasma oxygen radical absorbance capacity | 2 hours post-prandial | 
| Plasma ox-LDL |  | 2 hours post-prandial | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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antioxidants, polyphenols, flow-mediated dilatation