Comparison of the Genotoxicity of Desflurane and Propofol

Study Overview
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Brief Summary
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The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.

Detailed Description
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It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.

Official Title
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Genotoxic Effects of Desflurane and Propofol Anesthesia in Patients Undergoing Lumbar Disc Surgery

Conditions
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DNA Damage, Anesthesia

Intervention / Treatment
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* Drug: Anesthetics, General Desflurane Maintenance
* Drug: Anesthetics, General Propofol Maintenance


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Ability to understand and willingness to sign an informed consent form Elective lumbar spinal surgery that will take at least 120 minutes, American Society of Anesthesiologists (ASA) physical status I and II Exclusion Criteria: Patients with ASA physical status III or IV disease Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders) Smoking or alcohol consumption Recently received radiation, chemotherapy Malignancy Patients who will receive blood transfusion during the operation Users of antioxidant supplements Patients who have received general anesthesia in the last three months Patients with known occupational exposures (operating room personnel, chemical plant worker

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Sequential Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Desflurane Group<br>Desflurane 1,0 minimum alveolar concentration will be administered during anesthesia maintenance | Drug: Anesthetics, General Desflurane Maintenance<br>* Comparison of dna damage of desflurane maintenance with propofol maintenance according to comet analysis<br>|
| Experimental: Propofol Group<br>Propofol 6 mg//kg/hour will be administered during anesthesia maintenance | Drug: Anesthetics, General Propofol Maintenance<br>* Comparison of dna damage of propofol maintenance with desflurane maintenance according to comet analysis<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | Before anesthesia induction | 
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | 2 hours after anesthesia induction | 
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | postoperative 1st day | 
| Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage. | postoperative 5th day | 
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. | Before anesthesia induction | 
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. | 2 hours after anesthesia induction | 
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. | postoperative 1 st day | 
| Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage. | postoperative 5th day | 
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | before anesthesia induction | 
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | 2 hours after anesthesia induction | 
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | postoperative 1 st day | 
| Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire | The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage. | postoperative 5th day | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Desflurane, Intravenous anesthetics, propofol, comet essay