Interleukin-6 Secretion in Adipose Tissue Following Sprint Exercise

Study Overview
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Brief Summary
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Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.

Detailed Description
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PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange. METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation. SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports

Official Title
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Metabolism in Adipose Tissue Followed Following Sprint Exercise

Conditions
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Metabolism in Adipose Tissue

Intervention / Treatment
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* Other: Repeated 30-s sprint exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Physical active Healthy Exclusion Criteria: Smokers Pregnant On medication No infection No asthma

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Sprint exercise<br>Three bouts of 30-s sprint exercise with 20 min rest in between. Three fat biopsies obtained ar rest before first sprint, 15 min after and 120 min after third sprint Blood samples from stomach vein and arteria during the whole experiment | Other: Repeated 30-s sprint exercise<br>* Analyze of Interleukin-6 concentration before and after repeated 30-s sprint exercise<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise | Stomach fat biopsies and arterial and stomach vein blood are obtained | Before 30-s repeated sprint exercise | 
| Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise | Stomach fat biopsies and arterial and stomach vein blood are obtained | After 30-s repeated sprint exercise | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in blood flow in adipose tissue | Xenon-133 is administered by a subcutaneous injection into the abdominal adipose tissue of approximately 1-2 MBq of Xenon-133 in the form of gas, at rest before work. | Before 30-s repeated sprint exercise | 
| Changes in blood flow in adipose tissue | The rinsing rate of Xenon-133 from the tissue depot is continuously recorded during the study period by a non-invasive method using a portable scintillation detector system (Mediscint) up to 60 minutes after the last sprint work. | After 30-s repeated sprint exercise | 
| Changes in different metabolites | Analysed for lactate, ammonia and IL-6 | Before 30-s repeated sprint exercise | 
| Changes in different metabolites | Analysed for lactate, ammonia and IL-6 | After 30-s repeated sprint exercise |