Leucine-enriched Essential Amino Acid Intake to Optimize Protein Anabolism in Children With Cystic Fibrosis

Study Overview
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Brief Summary
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Malnutrition, including muscle wasting commonly occurs in children with cystic fibrosis (CF), negatively influencing their quality of life and survival. At the time of a diagnosis of CF, severe protein deficits can already be present. It is important to get CF children fed adequately to prevent that their condition becomes worse or that recovery takes longer. Oral supplementation trials showed that gains in lean body mass are difficult to achieve in CF unless specific metabolic abnormalities are targeted. However, the specific needs for certain food components are not clear yet in children that are ill. Therefore, more information is necessary on the need for protein and certain amino acids in children with CF. Previous studies support the concept of essential amino acids (EAA) as an anabolic stimulus in the young and elderly and in insulin resistant states. Until yet no information is present on the anabolic effects of EAA in CF. It is therefore our hypothesis that a high-leucine essential amino acids mixture specifically designed to stimulate protein anabolism will target the metabolic alterations of pediatric subjects with CF. In the present proposal, the acute metabolic effects of this high leucine essential amino acids mixture will be examined in pediatric subjects with CF and compared to that of a regular balanced total mixture of essential and non-essential amino acids. The principal endpoints will be the extent of stimulation of whole body protein synthesis as this is the principal mechanism by which either amino acid or protein intake causes muscle anabolism, and the reduction in endogenous protein breakdown. Both endpoints will be assessed by isotope methodology which is thought to be the reference method.

Detailed Description
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In this study, we will test the following hypothesis: A high-leucine essential amino acid mixture (dose of 6.7 g) will stimulate protein anabolism to a greater extent than a standard balanced mixture of total (essential and non-essential) amino acids in CF pediatric subjects. The principal endpoints will be the extent of stimulation of protein synthesis rate and the reduction in endogenous protein breakdown. The current project will provide information that will enable us to better understand the underlying metabolic mechanisms that regulate protein metabolism in pediatric subjects with CF.

Official Title
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Leucine-enriched Essential Amino Acid Intake to Optimize Protein Anabolism in Children With Cystic Fibrosis

Conditions
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Cystic Fibrosis

Intervention / Treatment
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* Dietary Supplement: Essential amino acid intake + Leucine vs total AA supplement


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subjects who already have a diagnosis of CF based on universal diagnostic criteria. Age 14 to 21 years at the time of enrollment Under routine medical control at the CF center of ACH Admitted to the ACH for treatment of pulmonary exacerbation of CF disease. Improvement in lung function (FEV1) at the time of enrollment back to baseline values (as determined in the clinically stable pre-hospital period) Central or peripheral venous line in place No planned major changes or interventions in the treatment and care of the pediatric subject on Day -2 and -1 before discharge from the hospital. Exclusion Criteria: Established diagnosis of Diabetes Mellitus Presence of fever within the last 3 days Unstable metabolic diseases including liver (cirrhosis) or renal disease Chronic respiratory failure with cor pulmonale Use of long-term oral corticosteroids or short course of oral corticosteroids in the preceding month before enrollment Any other condition according to the principle investigator or study physician would interfere with collecting study samples Failure to give assent / informed consent

Ages Eligible for Study
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Minimum Age: 10 Years
Maximum Age: 21 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Allocation: Randomized
Intervention Model: Single Group Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Oral EAA vs total AA supplement<br> | Dietary Supplement: Essential amino acid intake + Leucine vs total AA supplement<br>* 7 g as bolus<br>* Other names: 7 g EAA + 40% LEU;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Net whole body protein synthesis rate | Acute change from postabsorptive state after intake of essential amino acid + LEU vs total amino acid supplement | Up to 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Whole body collagen breakdown rate | Acute change from postabsorptive state after intake of essential amino acid + LEU vs total amino acid supplement | Up to 2 years | 
| Urea turnover rate | Acute change from postabsorptive state after intake of essential amino acid + LEU vs total amino acid supplement | Up to 2 years | 
| Arginine turnover rate | Measured in postabsorptive state | Up to 2 years | 
| Liver protein synthesis rate | Acute change from postabsorptive state after intake of essential amino acid + LEU vs total amino acid supplement | Up to 2 years | 
| Resting Energy expenditure | Measured in postabsorptive state | Up to 2 years | 
| Insulin kinetics | Acute change from postabsorptive state after intake of essential amino acid + LEU vs total amino acid supplement | Up to 2 years | 
| Amino acid kinetics | Acute change from postabsorptive state after intake of essential amino acid + LEU vs total amino acid supplement | Up to 2 years | 
| Glucose kinetics | Acute change from postabsorptive state after intake of essential amino acid + LEU vs total amino acid supplement | Up to 2 years | 
| Fat-free mass | Characterization of subjects | Up to 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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CF, protein metabolism, essential amino acid intake