Analysis of Protein and Emotional Alterations During and After Hypobaric Hypoxia

Study Overview
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Brief Summary
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This study analyses both Protein Expression and emotional alterations during hypobaric hypoxia in volunteers of different groups.

Detailed Description
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This study analyses both Protein Expression and emotional alterations during hypobaric hypoxia in volunteers of different groups. 15 different Groups are analysed: control Group, 5000ft Group, 8000 ft Group, 10000ft Group and 15000ft Group - all for 15, 30, and 60 minutes.

Official Title
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Analysis of Protein and Emotional Alterations During and After Hypobaric Hypoxia

Conditions
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Hypoxia, Altitude

Intervention / Treatment
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* Procedure: Hypoxia


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Helathy Exclusion Criteria: Ear problems

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Randomized
Intervention Model: Single Group Assignment
Interventional Model Description: 15 different Groups are analysed: control Group, 5000ft Group, 8000 ft Group, 10000ft Group and 15000ft Group - all for 15, 30, and 60 minutes.
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: control group 15<br>Protein expression and emotional test 15 minutes normoxia Interventions: Blood drawing 1 Minute before and 1 Minute after the pressure chamber Phase for Proteomic profiling with 2D-gels Emotional tests (tears, stroop, mood) during the pressure chamber phase | |
| No Intervention: control group 30<br>30 minutes normoxia Interventions: Blood drawing 1 Minute before and 1 Minute after the pressure chamber Phase for Proteomic profiling with 2D-gels Emotional tests (tears, stroop, mood) during the pressure chamber phase | |
| No Intervention: control group 60<br>60 minutes normoxia Interventions: Blood drawing 1 Minute before and 1 Minute after the pressure chamber Phase for Proteomic profiling with 2D-gels Emotional tests (tears, stroop, mood) during the pressure chamber phase | |
| Experimental: hypoxia 5000ft group 15<br>Intervention: 15 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 5000ft group 30<br>Intervention: 30 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 5000ft group 60<br>Intervention: 60 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 8000ft group 15<br>Intervention: 15 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 8000ft group 30<br>Intervention: 30 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 8000ft group 60<br>Intervention: 60 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 10000ft group 15<br>Intervention: 15 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 10000ft group 30<br>Intervention: 30 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 10000ft group 60<br>Intervention: 60 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 15000ft group 15<br>Intervention: 15 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 15000ft group 30<br>Intervention: 30 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|
| Experimental: hypoxia 15000ft group 60<br>Intervention: 60 minutes hypoxia | Procedure: Hypoxia<br>* hypoxia<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Protein Expression before hypoxia | proteomic profiling of Serum by 2D-gels. All Proteins present in the sample will be identified by proteomics | Measurement immediately 1 minute before of hypoxia/experiment | 
| Protein Expression after hypoxia | proteomic profiling of Serum by 2D-gels. All Proteins present in the sample will be identified by proteomics | Measurement immediately and at termination (i.e., 1 minute after) of hypoxia/experiment | 
| Emotional assessment tears | Emotional tears test | Measurement starts immediately with hypoxia in the pressure chamber. duration 10 minutes | 
| Emotional assessment | Emotional stroop test; Williams, J. M. G., Mathews, A., & MacLeod, C. (1996). The emotional Stroop task and psychopathology. Psychological Bulletin, 120, 3-24. | Measurement in the pressure chamber after tears test (i.e. 10 minutes after start). duration 10 minutes | 
| Emotional assessment mood | Emotional mood test, POMS mood score. Shukitt-Hale, B., Banderet, L.E., & Lieberman, H.R. (1998). Elevation-dependent symptom, mood, and performance changes produced by exposure to hypobaric hypoxia, The International Journal of Aviation Psychology, 8, 319-334, | Measurement in the pressure chamber after stroop test (i.e. 20 minutes after start). duration 10 minutes |