Blood Samples From Patients With Non-Small Cell Lung Cancer and From Healthy Volunteers

Study Overview
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Brief Summary
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RATIONALE: Studying samples of blood from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at blood samples from patients with non-small cell lung cancer and from healthy volunteers.

Detailed Description
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OBJECTIVES: Measure plasma cathepsin D levels in patients with non-small cell lung cancer and in healthy volunteers using mass spectometry. Measure fatty acid synthase levels in these patients and healthy volunteers using mass spectometry. OUTLINE: Blood samples are collected from patients and healthy volunteers to measure the levels of cathepsin D and fatty acid synthase by proteomic profiling and mass spectrometry.

Official Title
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Plasma Quantification of Cathepsin D and FAS in Non-Small Cell Lung Cancer

Conditions
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Lung Cancer

Intervention / Treatment
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* Other: Mass Spectrometry


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Non-Small Cell Lung Cancer Patients Documented diagnosis of non-small cell lung cancer, any stage and any time point in the course of treatment (i.e., at diagnosis, during any treatment, and during post treatment surveillance) Measurable disease by CT scan within the past 4 weeks Healthy volunteer Non-smoking, defined as < 5 packs/year history of smoking and cessation of smoking ≥ 6 months ago OR a never smoker Not regularly exposed (i.e., daily) to second-hand smoke Exclusion Criteria: Severe underlying lung disease (i.e., chronic obstructive pulmonary disease with FEV_1 < 1.0 L, pulmonary fibrosis, bronchiectasis, cystic fibrosis, etc.) as determined by enrolling researcher. Written consent must be given for both subjects and controls.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy Volunteers<br>Non-smoking healthy volunteers | Other: Mass Spectrometry<br>* Levels of Cathepsin D (CD) and fatty acid synthase (FAS) will be measured using isobaric tag labeling coupled with mass spectrometry. RNA concentration will be measured using spectrophometer and purity will be analyzed.<br>|
| Non-Small Cell Lung Cancer<br>Patients with non-small cell lung cancer. | Other: Mass Spectrometry<br>* Levels of Cathepsin D (CD) and fatty acid synthase (FAS) will be measured using isobaric tag labeling coupled with mass spectrometry. RNA concentration will be measured using spectrophometer and purity will be analyzed.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Levels of cathepsin D | Measure plasma CD levels in patients with non-small cell lung cancer and non-smoker healthy controls using mass spectrometry. | At Routine Follow-Up | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Levels of fatty acid synthase (FAS) | Measure FAS levels in patients with non-small cell lung cancer and non-smoker healthy controls using mass spectrometry. | At Routine Follow-Up | 
| Global microRNA expression | Measure global microRNA expression by microarray analysis using Illumina probes. | At Routine Follow-Up | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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recurrent non-small cell lung cancer, stage I non-small cell lung cancer, stage II non-small cell lung cancer, stage IIIA non-small cell lung cancer, stage IIIB non-small cell lung cancer, stage IV non-small cell lung cancer