Analysis of the Influence of Desensitizing Treatments on the Pulp Inflammatory Response in Bleached Teeth

Study Overview
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Brief Summary
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This randomized, controlled, blinded clinical trial evaluated the effectiveness of different desensitizing protocols in preventing pulp inflammation after bleaching treatment with 35% hydrogen peroxide (PH) (Whiteness PH 35%).

Detailed Description
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Thirty healthy third human molars extracted by orthodontic indication were randomized and allocated into five groups (n = 6): CN (negative control): without intervention; CP (Positive control): PH; PMB: PH + photobiomodulation with low power laser; CPP: PH + casein phosphopeptide phosphopeptide amorphous calcium phosphate (CPP-ACPF); NANO: PH + nanohydroxyapatite. The office whitening was performed in two sessions with a single application of 45 min and an interval of 48 hours between them. Pulp tissues were removed for immunohistochemical analysis. Immunostaining was performed for Caspase 3 using an Axio Scope.A1 microscope.

Official Title
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Effect of Different Desensitizing Protocols on the Pulp Inflammatory Response in Bleached Teeth: A Randomized Clinical Trial.

Conditions
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Pulp Inflammation

Intervention / Treatment
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* Other: Whiteness HP 35%
* Device: Whiteness HP 35% + LASER
* Other: Whiteness HP 35% + CPP
* Other: Whiteness HP 35% + NANO


Participation Criteria
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Eligibility Criteria
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Inclusion criteria: Lower third molars class I position A Maxillary third molars horizontal position A Absence of caries and enamel fracture Absence of periodontal disease Extraction of third molars for orthodontic reasons No allergy to the anesthetic and positive pulp sensitivity test Exclusion criteria: Calcification in the middle third of the root Drug users or smokers Patients who have already done whitening Root apex with incomplete formation and patients with systemic problems that would make surgery impossible

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 26 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Sequential Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Positive Control Group<br>Whiteness HP 35% | Other: Whiteness HP 35%<br>* Single application of 45 minutes on the buccal surfaces of third molars, with an average thickness of 1 mm<br>|
| Experimental: Laser Group<br>Whiteness HP 35% + Laser | Other: Whiteness HP 35%<br>* Single application of 45 minutes on the buccal surfaces of third molars, with an average thickness of 1 mm<br>Device: Whiteness HP 35% + LASER<br>* A dose of LASER (60 J / cm2, for 16 seconds) was applied at two points, one point in the cervical region and another in the center of the buccal surface of the third molars, using visible infrared LASER therapy (Photon III / DMC, São Carlos, SP, Brazil), with a wavelength of 808 nm, and AsGaAl, as an active medium.<br>|
| Experimental: CPP Group<br>Whiteness HP 35% + CPP | Other: Whiteness HP 35%<br>* Single application of 45 minutes on the buccal surfaces of third molars, with an average thickness of 1 mm<br>Other: Whiteness HP 35% + CPP<br>* Topical application of casein phosphopeptide amorphous calcium phosphate fluoride CPP-ACPF (My Paste Plus, Recaldent, GC, USA) with the aid of a microbrush (KG Sorensen, SP, Brazil) in a passive way for 5 minutes.<br>|
| Experimental: Nano Group<br>Whiteness HP 35% + NANO | Other: Whiteness HP 35%<br>* Single application of 45 minutes on the buccal surfaces of third molars, with an average thickness of 1 mm<br>Other: Whiteness HP 35% + NANO<br>* A topical application of nanohydroxyapatite (Nano P FGM, SC, Brazil) was performed with the aid of a microbrush (KG Sorensen, SP, Brazil) in a passive way for 5 minutes after teeth whitening.<br>|
| No Intervention: Negative Control<br>Without intervention | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Pulp inflammation | Evaluation of pulp inflammation through immunohistochemistry of the third molar whitening or not | 3 days After extraction of third molars | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Therapies, Caspase 3, Immunohistochemistry