Acute Effects of Natural and Interesterified Fats-margarine on Lipemic and Insulinemic Response

Study Overview
=================
Brief Summary
-----------------
Interesterified (IE) fat blends will respond differently in terms of postprandial lipemia and insulinemic responses compare to a trans free palm margarine (TFPM).

Detailed Description
-----------------
The objective of this study was to investigate whether IE-fat blends behave differently on postprandial serum lipid, insulin, C-peptide and glucose concentration compare to TFPM. A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults. During postprandial challenge day, each subject received an a test muffin incorporated with test margarine. Blood samples were collected at half-hourly intervals until 4 hr by using cannulation technique.

Official Title
-----------------
Acute Effects of Natural and Interesterified Fats-formulated Margarine on Lipemic and Insulinemic Response

Conditions
-----------------
Healthy

Intervention / Treatment
-----------------
* Other: Trans free palm margarine
* Other: Interesterified Palm based margarine
* Other: IE Soybean oil-based margarine


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Body Mass Index (BMI) between 18.5 and 24.9 <130mmHg for systolic pressure and <80 mmHg for diastolic pressure Blood glucose level in the range of 3.9 to 5.5 mmol/L Total cholesterol less than 5.17 mmol/L No use of tobacco and no consumption of alcohol. Exclusion Criteria: Suffer from chronic disease Metabolic syndrome Consuming any medical prescriptions People with blood clotting problem Women who are pregnant Hypertensive person

Ages Eligible for Study
-----------------
Minimum Age: 20 Years
Maximum Age: 50 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Trans free palm margarine<br>One high fat muffin will be serves together with a glass of low fat milk shake. | Other: Trans free palm margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>Other: Interesterified Palm based margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>Other: IE Soybean oil-based margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>|
| Experimental: Interesterified palm based margarine<br>One high fat muffin will be serves together with a glass of low fat milk shake. | Other: Trans free palm margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>Other: Interesterified Palm based margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>Other: IE Soybean oil-based margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>|
| Experimental: IE soybean oil-based margarine<br>One high fat muffin will be serves together with a glass of low fat milk shake. | Other: Trans free palm margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>Other: Interesterified Palm based margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>Other: IE Soybean oil-based margarine<br>* A randomised, double-blind crossover (3 × 3 arms) orthogonal Latin-square design was used and conducted on 10 males and 10 females adults.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in serum triglycerides level of subjects as a measure of postprandial triglycerides response |  | Baseline, 4 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in the level of plasma insulin, c-peptide and serum glucose as a measure of postprandial insulinemic response |  | Baseline, 4 hours |