Alterations in Subgingival Microbiota and Hypoxia in Occlusal Trauma

Study Overview
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Brief Summary
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Present study suggests that excessive occlusal forces did not cause a significant change in hypoxia and vascular markers, however a slight alteration in subgingival microbiota was observed.

Detailed Description
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Occlusal trauma is defined as tissue destruction and/or injury resulting in tissue changes in the periodontium as a consequence of excessive occlusal forces or hyper-occlusion. Previous studies suggested accelerated periodontal destruction around teeth with occlusal trauma and increased mobility, however, the evidence is lacking. Recent animal studies found that occlusal trauma increased osteoclastic activity and alveolar bone loss. One possible mechanism suggested for the progression of periodontal destruction with occlusal trauma is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. However, the association of occlusal trauma and subgingival microbiota was not evaluated in previous studies. Therefore, present study aimed to evaluate subgingival dental plaque components and compare with healthy individuals and periodontitis patients without occlusal trauma.

Official Title
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Alterations in Subgingival Microbiota and Hypoxia in Occlusal Trauma

Conditions
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Chronic Periodontitis, Occlusal Trauma

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Systemical health No drug use No pregnancy or lactation For group 3 and 4; Bruxism at least for 2 years Occlusal trauma Existence of at least 20 functioning teeth Exclusion Criteria: Smoking Pregnancy/lactation Systemical disease Drug use Absence of at least 20 functioning teeth

Ages Eligible for Study
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Minimum Age: 29 Years
Maximum Age: 42 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| healthy individuals<br>Orally and systemically healthy individuals who has no hypo-functional teeth, hyper-occlusion or occlusal trauma | |
| periodontitis patients<br>Systemically healthy individuals who has chronic periodontitis but no hypo-functional teeth, hyper-occlusion or occlusal trauma | |
| healthy-occlusal trauma<br>Orally and systemically healthy individuals who has occlusal trauma caused by bruxism | |
| periodontitis-occlusal trauma<br>Systemically healthy individuals who has both chronic periodontitis and occlusal trauma caused by bruxism | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determination of certain bacterial species in subgingival dental plaque | Subgingival dental plaque samples were collected from each participant and evaluated via polymerase-chain reaction. | A day after initial examination | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| hypoxic changes in gingiva and serum | Determination of the hypoxia inducible factor-alpha and vascular endothelial growth factor via ELISA | One day after initial examination | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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hyper-occlusion, hypoxia, occlusal trauma, periodontitis, subgingival microbiota