The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization (IVF) Outcome of Sibling Oocytes

Study Overview
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Brief Summary
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The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.

Detailed Description
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This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media: Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult) P1 medium vs. ECM medium (Irvine). Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.

Official Title
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The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization Outcome of Sibling Oocytes

Conditions
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Infertility

Intervention / Treatment
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* Other: Sibling oocyte incubation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Sibling oocytes from cycles where at least 4 oocytes were retrieved Exclusion Criteria: Less than 4 oocytes retrieved Partner has undergone testicular sperm extraction (TESE)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 42 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Universal IVF Medium<br>An oocyte culture medium containing 5.55 Mm glucose (Origio, Medi-Cult) | Other: Sibling oocyte incubation<br> <br> |
| ISM1 Medium<br>An oocyte culture medium containing 1mM glucose ((Origio, MediCult) | Other: Sibling oocyte incubation<br> <br> |
| P1 Medium<br>An oocyte culture medium containing no glucose (Irvine) | Other: Sibling oocyte incubation<br> <br> |
| ECM Medium<br>An oocyte culture medium containing 0.5mM glucose (Irvine) | Other: Sibling oocyte incubation<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Fertilization Rate | Fertilization rates will be retrospectively compared according to culture media in which sibling oocytes were incubated | 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Embryo Quality | Embryo quality and developmental rate will be retrospectively compared according to culture media in which sibling oocytes were incubated | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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sibling oocytes, glucose, culture media