Study of Acute Effects of Sn-1 and Sn-3 Palmitic Acid-rich or Stearic Acid-rich Fats on Metabolic Markers

Study Overview
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Brief Summary
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To determine the effect of degree of fat saturation on postprandial lipemia, haemostatic and inflammatory markers, gut hormone concentrations, satiety perceptions, and insulinemic responses in healthy adult subjects. Study hypothesis: The degree of fat saturation does not affect postprandial lipemia, haemostatic and inflammatory markers, gut hormone concentrations, insulinemic or satiety responses in health adult subjects.

Detailed Description
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The experimental are comprise of 50g fat, 14g protein and 84g carbohydrate. Each of the test fat [palm mid fraction (PMF), shea butter (SB) and high oleic sunflower oil (HOSF)] will be compared which will be in a form of experimental meals (muffin). 33 volunteers receives 3 experimental meals (muffins) in a random order, 2 week apart, over a 6-week period. They will be randomized, blinded and will undergo a 3x3 arms crossover. The first 15-ml (0 min) fasting venous blood samples is obtained. Immediately thereafter, the subject consumes the test meal plus a glass of water within 15 minutes. The time sequence is started mid-way of consuming the test meal. At 30 min, 60 min, 90, 120 min (2 hr), 150 min and 180 min (3 hr), 210 min, and 240 min (4 hr), 15-ml each of blood is collected (via a vascular catheter inserted earlier in the subjects hand/arm).

Official Title
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Acute Effects of Sn-1 and Sn-3 Palmitic Acid-rich or Stearic Acid-rich Fats on Postprandial Lipemia, Haemostatic and Inflammatory Markers, Gut Hormone Concentrations, Satiety, and Insulinemic Response in Human Volunteers

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Palm Mid Fraction, Shea Butter, High Oleic Sunflower Oil


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy adult male or female staff, aged between 25-50 years BMI 18.5 - 25.0 kg/m2 Blood pressure: Systolic: <140 mmHg, diastolic: <90 mmHg c. Normolipidemic (Total cholesterol <6.2 mM/L (<240 mg/dL), Fasting TG <1.70 mM/L (<150 mg/dL) d. Fasting glucose 4.0- 7.0 mM/L Exclusion Criteria: Smokers On cholesterol or blood glucose medication Substance abuse (eg. alcohol, cigarette smoking) For women, pregnant or lactating Going overseas during period of study Blood clotting problem Public transportation to work

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Palm Mid Fraction<br> | Dietary Supplement: Palm Mid Fraction, Shea Butter, High Oleic Sunflower Oil<br>* Acute effect of sn-1 and sn-3 palmitic acid-rich or stearic acid-rich fats on postprandial lipemia, haemostatic and inflammatory markers, gut hormone concentrations, satiety, and insulinemic response in human volunteers ; 6 wk each with 2 week wash out in between<br>* Other names: POP vs SOS vs OOO;|
| Experimental: Shea Butter<br> | Dietary Supplement: Palm Mid Fraction, Shea Butter, High Oleic Sunflower Oil<br>* Acute effect of sn-1 and sn-3 palmitic acid-rich or stearic acid-rich fats on postprandial lipemia, haemostatic and inflammatory markers, gut hormone concentrations, satiety, and insulinemic response in human volunteers ; 6 wk each with 2 week wash out in between<br>* Other names: POP vs SOS vs OOO;|
| Experimental: High Oleic Sunflower Oil<br> | Dietary Supplement: Palm Mid Fraction, Shea Butter, High Oleic Sunflower Oil<br>* Acute effect of sn-1 and sn-3 palmitic acid-rich or stearic acid-rich fats on postprandial lipemia, haemostatic and inflammatory markers, gut hormone concentrations, satiety, and insulinemic response in human volunteers ; 6 wk each with 2 week wash out in between<br>* Other names: POP vs SOS vs OOO;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| glucose-dependent insulintropic polypeptide (GIP) |  | 4 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Lipid profile |  | 4 hours | 
| Inflammatory markers |  | 4 hours | 
| Haemostatic indices |  | 4 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Palm mid fraction, Shea butter, sn-1, sn-3 fatty acids, Cholesterol, Postprandial lipemia,, Inflammatory markers, Satiety, Gut hormone, Insulin