Determination of Adipocytokine in Synovial Fluid of Osteoarthritis Patients

Study Overview
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Brief Summary
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The purpose of this study is to determine the concentration of different adipocytokine (adiponectin, leptin, resistin, visfatin, osteopontin) and an inflammation classic markers as Interleukine 1 beta, tumor necrosis alfa, interleukine 6 and high sensibility reactive c protein in the synovial fluid of knee osteoarthritis patients, and compare with the inflammation detected with ultrasonography evaluation of the joint. In last years, some studies appeared focusing on the role of adipocytokines in osteoarthritis and they correlated adipocytokines with cartilage degradation and x-ray degree; in our study we will focused in the importance of adipocytokines in the inflammatory changes more than in joint destruction.

Official Title
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Determination of Adipocytokine in Synovial Fluid of Osteoarthritis Patients With Joint Pain and Effusion

Conditions
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Osteoarthritis

Intervention / Treatment
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* Other: arthrocentesis
* Other: ultrasonography of the knee


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: aged 50 to 81 primary osteoarthritis of the knee synovial effusion in examination patient who have the results in our database Exclusion Criteria: any other rheumatic conditions as rheumatoid arthritis or another inflammatory disease secondary knee osteoarthritis history of knee surgery any conditions that at investigator criteria exclude the patient from study procedures

Ages Eligible for Study
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Minimum Age: 50 Years
Maximum Age: 81 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Inflammatory knee osteoarthritis<br>We recruited patients with osteoarthritis and synovial effusion to diagnostic the effusion and to confirm that this this synovial fluid had mechanical characteristics. Now we are going to determine the adipocytokines and traditional parameters of inflammation in this synovial fluid to correlate it, and we will associate it with the ultrasound explanation of the knee that we have in our database. | Other: arthrocentesis<br>* The made a diagnostic arthrocentesis to all patient we included<br>Other: ultrasonography of the knee<br>* We explored the affected knee with an ultrasound explanation in order to describe inflammatory changes based on standard examination.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determination of adipocytokine levels (adiponectin, leptin, resistin, visfatin, osteopontin) in synovial fluid | We have all the synovial fluid freeze, so now we have to determine the levels of everyone. | We determine adipocytoquines in one month, all patients in the same determination kit | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Knee inflammation parameters detected by ultrasonography | We performed the ultrasonography evaluation at the same time than the visit were realized, and we have the results in a data base, so we will wait for the adipocytokine results and we will compare with ultrasound evaluation | three months | 
| To determine additional classic parameters of inflammation in synovial fluid as Interleukine 1 beta, Interleukine 6 and tumor necrosis factor alfa | We will use classic inflammation parameters to compare with adipocytokine levels | We determine this inflammatory parameters in one month in all patient at the same time | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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adipocytokines, osteoarthritis, inflammation, ultrasonography