GPx Activity in Subjects With Aortic Stenosis Undergoing TAVR

Study Overview
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Brief Summary
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The aim of this project is to investigate the association of glutathione peroxidase (GPx) and severe aortic stenosis (AS), as well as the impact of transcatheter aortic valve replacement (TAVR) on GPx activity post-procedure. The burden of oxidative stress will be determined by the measurement of GPx, superoxide dismutase (SOD) and lipoprotein A (Lp(a)). We hypothesize GPx activity is reduced in participants with severe AS vs control groups and GPx activity is to increase after TAVR is performed.

Official Title
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Glutathione Peroxidase Activity in Subjects With Aortic Stenosis Undergoing Transcatheter Aortic Valve Replacement.

Conditions
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Glutathione Peroxidase Activity, Aortic Stenosis, Transcatheter Aortic Valve Replacement

Intervention / Treatment
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* Diagnostic Test: Blood draw to measure GPx activity
* Diagnostic Test: Blood draw to measure SOD activity
* Diagnostic Test: Blood draw to measure Lp(a) levels


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age >18 years Patients with severe AS including participants with low flow, low gradient severe AS and paradoxical severe AS Patients meeting criteria for TAVR procedure Exclusion Criteria: Previous aortic valve replacement (surgical or TAVR) Participants undergoing chemotherapy End stage liver disease/cirrhosis (liver produces GSH)

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients with severe aortic stenosis undergoing TAVR<br>GPx activity levels will be measured on the day of TAVR procedure, the day of discharge, 1 month, and 6 months after the procedure | Diagnostic Test: Blood draw to measure GPx activity<br>* Routine blood draw to measure levels of blood markers.<br>Diagnostic Test: Blood draw to measure SOD activity<br>* Routine blood draw to measure levels of blood markers.<br>Diagnostic Test: Blood draw to measure Lp(a) levels<br>* Routine blood draw to measure levels of blood markers.<br>|
| Patients without aortic stenosis<br>GPx activity levels will be measured on day of recruitment | Diagnostic Test: Blood draw to measure GPx activity<br>* Routine blood draw to measure levels of blood markers.<br>Diagnostic Test: Blood draw to measure SOD activity<br>* Routine blood draw to measure levels of blood markers.<br>Diagnostic Test: Blood draw to measure Lp(a) levels<br>* Routine blood draw to measure levels of blood markers.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Level of GPx in blood samples | Glutathione peroxidase activity levels measured from whole blood in U/L units in participants with severe aortic stenosis after transcatheter valve replacement compared with the matched population | Baseline up to 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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glutathione peroxidase, GPx, aortic stenosis, transcatheter aortic valve replacement, TAVR