Mycoprotein Consumption and Glucose Absorption

Study Overview
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Brief Summary
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Previous research has demonstrated that the food found in Quorn 'Mycoprotein' can lower the blood glucose response to a meal, which may be an important finding for the management of type 2 diabetes. However, it has never been investigated how this lower response occurs, and whether there is dose-dependent response with mycoprotein. The present study will include 12 healthy young adults, who will visit the laboratory for 3 test days, with each day lasting 9 hours. On each visit, separated by a washout period of at least 2 weeks, participants will ingest either a control drink with no mycoprotein, a drink containing 20g mycoprotein or a drink containing 40g mycoprotein. By intravenously infusing labelled glucose during each visit in combination with repeated blood sampling, investigators will determine if the lower blood glucose after mycoprotein ingestion is due to reduced glucose absorption from the gut or increased uptake into body tissues.

Official Title
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To Determine the Effects of Mycoprotein Consumption on Glucose Absorption in Healthy Participants

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Mycoprotein Ingestion


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male and female 18-35 years of age Consumption of sufficient amounts of carbohydrate in the diet Body mass index between 18.5 and 30 Exclusion Criteria: Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes), or other form of abnormal glucose metabolism (as this will affect outcome of the study). Any diagnosed cardiovascular disease Insufficient consumption of carbohydrate in the diet A personal or family history of epilepsy, seizures or schizophrenia Known pre-existing liver disease/condition Allergy or intolerance to milk

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Ingestion of 20g Mycoprotein Drink<br>Milkshake containing 20g of mycoprotein, 250ml full-fat milk, 50g glucose and 11g lactose is given to participants in one dose | Dietary Supplement: Mycoprotein Ingestion<br>* Ingestion of various doses of mycoprotein compared to placebo control<br>|
| Experimental: Ingestion of 40g Mycoprotein Drink<br>Milkshake containing 40g of mycoprotein, 250ml full-fat milk, 50g glucose and 11g lactose is given to participants in one dose | Dietary Supplement: Mycoprotein Ingestion<br>* Ingestion of various doses of mycoprotein compared to placebo control<br>|
| Placebo Comparator: Ingestion of Isocaloric Control Drink<br>Milkshake containing 250ml full-fat milk, 50g glucose, 19g dried skim milk and 9g full-fat dried milk | Dietary Supplement: Mycoprotein Ingestion<br>* Ingestion of various doses of mycoprotein compared to placebo control<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glucose absorption | How glucose is absorbed following the ingestion of a mycoprotein drink compared to a placebo control measured using the infusion of stable isotopes | 6 hours following ingestion of drink | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Incretin Hormones | Measurement of glucagon-like peptide-1(GLP-1) and gastric inhibitory polypeptide (GIP) in response to mycoprotein ingestion compared to placebo control | 6 hours following ingestion of drink | 
| Insulinaemic Response | Measurement of insulin response following ingestion of drinks | 6 hours following ingestion of drink | 
| Production of Short-Chain Fatty Acids | Measurement of plasma acetate production in response to mycoprotein ingestion | 6 hours following ingestion of drink | 
| Glucose Kinetics | Calculations using plasma deuterated glucose enrichment to determine rate of appearance of endogenous glucose, rate of disappearance of glucose and rate of appearance of exogenous glucose | 6 hours following ingestion of drink |