Interrupting Prolonged Sitting With Periodic Interval Exercise: Effect on Postprandial Lipemia

Study Overview
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Brief Summary
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Investigating the effects of interrupting various lengths of prolonged sitting with bike sprints on post prandial lipemia the next day. We will conduct three trials, one control, one with two-hour sitting intervals (total of 4 bouts of bike sprints) and one with 6-hour sitting intervals I total of two bouts of bike sprints). A milkshake high fat tolerance test will be conducted the next day and whole body fat oxidation as well as triglyceride area under the curve will be measured every hour for the duration of the 6-hour test.

Official Title
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Interrupting Prolonged Sitting With Periodic Interval Exercise: Effect on Postprandial Lipemia

Conditions
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Sedentary Behavior, Hypertriglyceridemia

Intervention / Treatment
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* Behavioral: Interrupting Prolonged Sitting with Periodic Interval Exercise: Effect on Postprandial Lipemia


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Inactive or recreational healthy adults aged 18-65 years old. Exclusion Criteria: Lactose intolerance, cardiovascular or metabolic diagnoses, medications for cardiovascular or metabolic diagnoses, orthopedic issues barring cycling.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: Control group-no exercise<br>A no-exercise control. Must maintain <4,500 steps per day for 3 consecutive days followed by a high fat tolerance test on day 4. | |
| Experimental: Exercise intervention 1<br>Two consecutive days of <4,500 steps per day followed by an intervention day on day 3. Intervention day consists of interrupting 8-hour sit with bike sprint protocol every 2 hours. Participants must aim to maintain <2,500 steps on intervention day. A high fat tolerance test will be performed the following day on day 4. | Behavioral: Interrupting Prolonged Sitting with Periodic Interval Exercise: Effect on Postprandial Lipemia<br>* This is a behavioral intervention designed to determine the effect of breaking up prolonged bouts of sitting every 2 hours and every 6 hours with 5 sets of 4 second bouts of maximum effort bicycle ergometer accelerations compared to sitting alone on the blood triglyceride response and whole body fat oxidation after a high fat meal.<br>|
| Experimental: Exercise intervention 2<br>Two consecutive days of <4,500 steps per day followed by an intervention day. Intervention day consists of interrupting 8-hour sit with bike sprint protocol every 6 hours. Participants must aim to maintain <2,500 steps on intervention day. A high fat tolerance test will be performed the following day on day 4. | Behavioral: Interrupting Prolonged Sitting with Periodic Interval Exercise: Effect on Postprandial Lipemia<br>* This is a behavioral intervention designed to determine the effect of breaking up prolonged bouts of sitting every 2 hours and every 6 hours with 5 sets of 4 second bouts of maximum effort bicycle ergometer accelerations compared to sitting alone on the blood triglyceride response and whole body fat oxidation after a high fat meal.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Triglyceride area under the curve | Blood measurements baseline and every hour during a high fat tolerance test. | 6 hour long high fat tolerance test | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Whole body fat oxidation | Expired gas collection and measurement of expired gas concentrations in mass spectrometer, baseline and every two hours during high fat tolerance test. | 10 min, baseline, 2 hours post ingestion of shake, 4 hours, 6 hours. |