Study of the Expression of MMPs in OME in Children With Atrophy of the Eardrum

Study Overview
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Brief Summary
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Metalloproteinases (MMPs) constitute a family of endopeptidases that cleaves the extracellular matrix as collagen included in the eardrum. Activity of MMP has been shown in some otitis media with effusion (OME) fluids. These enzymes could be directly linked to the prosnostic of OME as it may damage the eardrum and leads to tympanic atrophy. The main goal of the study is to find out the correlation between activity of MMPs and tympanic atrophy. The investigator will also study if there is any clinical predictive factors in relation to the level of MMPs.

Detailed Description
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The investigator will include 35 children under 12 years old with OME and who need tympanostomy or tube placement under general anesthesia. Middle ear effusion will be collected, divided in two and frozen. The investigator will perform ELISA tests to measure the level of MMP2 MMP9 MMP7 et TIMP2 (inhibitor of MMPs). The investigator want to compare the level of MMPs between healthy eardrum and atrophic membrane. Clinical data will be collected such as age, gender, chronicity of the OME, quality of the eardrum and middle ear effusion type to see if there is any difference when MMPs level is higher.

Official Title
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Study of the Expression of MMPs (Metalloproteinase Matricial) in OME (Otitis Media With Effusion) in Children Under Twelve Years Old With Atrophy of the Eardrum

Conditions
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Otitis Media With Effusion

Intervention / Treatment
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* Other: preservation of mucosal otitis media


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - every children under 12 yo operated on in Gui de Chauliac Hospital Exclusion criteria: - when effusion is not possible to collect (too thick or amount of fluid not enough) Collection of both MEE on each ear when OME is bilateral. We will perform the tests on only one ear. The other one will be kept in case of technical issue. A random draw will establish which ear will be tested.

Ages Eligible for Study
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Maximum Age: 12 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy eardrum<br>OME (otitis media with effusion)with normal tympanic membrane | Other: preservation of mucosal otitis media<br>* Suction of the middle ear fluid and conservation of the fluid to be collected instead of thrown away into children Healthy eardrum and Atrophic eardrum<br>|
| Atrophic eardrum<br>OME (otitis media with effusion) with atrophic membrane : thinning of the membrane, retraction pocket | Other: preservation of mucosal otitis media<br>* Suction of the middle ear fluid and conservation of the fluid to be collected instead of thrown away into children Healthy eardrum and Atrophic eardrum<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Microscopic evaluation of the quality of the eardrum : normal or atrophic | Microscopic evaluation of the quality of the eardrum : normal or atrophic | before surgery | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| biochemical assay of the level of TIMP | biochemical assay of the level of TIMP | 1 day | 
| biochemical assay of the level of MMPs | biochemical assay of the level of MMPs | 1 day |