Predictive Value of Human Microbiome and Serological Markers for Clinical Outcome of Cerebral Hemorrhage

Study Overview
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Brief Summary
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Objective: To explore the predictive value of characteristic disorder of intestinal flora for clinical prognosis in patients with intracerebral hemorrhage. Secondary objectives: 1) To investigate the correlation of gut microbiota and its serological indicators with imaging features and clinical neurological deficits in ICH; 2) Dynamically observe the changes of human microbiome and its serological indicators after ICH, and explore the biomarkers based on human microbiome related to disease changes.

Detailed Description
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Spontaneous intracerebral hemorrhage has brought a heavy burden to society and families. Finding biomarkers closely related to the condition of intracerebral hemorrhage is an important research direction for the prevention and treatment of intracerebral hemorrhage. However, there are few studies on the correlation between cerebral hemorrhage and microbiota. Our previous small sample study found that there were intestinal flora and SCFAs metabolism disorders in patients with hypertensive cerebral hemorrhage, and the latter was significantly related to poor prognosis. These results suggest that gut microbiota and its metabolites may become prognostic predictors and therapeutic targets for patients with intracerebral hemorrhage. However, more research evidence is still needed to confirm. Therefore, this study hypothesized that oral or intestinal flora in patients with acute cerebral hemorrhage has a certain degree of disorder and dynamic changes, accompanied by changes in serum markers, and there is a potential relationship between the changes of some microbiota-related markers and the severity and outcome of cerebral hemorrhage. Therefore, this topic proposed collection in patients with acute cerebral hemorrhage oral swabs, blood and feces or anal swab specimens, evaluate the neurological function score, imaging features and clinical outcomes, and to establish a follow-up queue, the dynamic change of cerebral hemorrhage patients after intestinal bacterial flora and condition and poor prognosis, the correlation of biomarkers new prediction and prevention of brain hemorrhage, To improve the effectiveness of prevention and treatment of cerebral hemorrhage.

Official Title
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Predictive Value of Human Microbiome and Serological Markers for Clinical Outcome of Cerebral Hemorrhage

Conditions
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Acute, Hemorrhagic Stroke, Outcomes, Metabolites, Gut Microbiota

Intervention / Treatment
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* Other: intestinal flora disturbance


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: It meets the diagnostic criteria for acute ischemic stroke Age ≥ 18 years The onset time is less than or equal to 2 weeks Diagnosis of cancer before stroke onset or during hospitalization and active cancer (failure to meet clinical criteria for cure, or discovery of recurrence or metastasis) Sign an informed consent form, provide relevant medical history information and provide biological specimens Exclusion Criteria: Previous history of disabling stroke (pre-onset mRS ≥2) Primary central nervous system tumor or hematologic system tumor The cancer meets the criteria for clinical cure and has not recurred or metastasized for more than 5 years Antibiotics, prebiotics/probiotics taken within 1 month Patients who cannot have stool specimens within 4 days of admission

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Acute cerebral hemorrhage<br>intestinal flora disturbance To observe the clinical prognosis of acute cerebral hemorrhage after enterobacterial disorder | Other: intestinal flora disturbance<br>* To observe the clinical prognosis of acute cerebral hemorrhage after enterobacterial disorder<br>|
| The group without stroke<br>The group without stroke | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Neurological function score (mRS score) | Neurological function score (mRS score) | 3 months after onset | 
| mortality | mortality | within 12 months after onset | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| New cerebrovascular events | Occurrence of ischemic stroke and hemorrhagic stroke | within 12 months after onset | 
| NIHSS | NIHSS | The first day and the seventh day after admission, 3 months, 6 months and 12 months after onset | 
| Barthel Index | BI | 3 months, 6 months and 12 months after onset | 
| Mini-mental State Examination | MMSE | The first day and the seventh day after admission, 3 months and 6 months after onset | 
| Montreal Cognitive Assessment | MOCA | The first day and the seventh day after admission、3 months and 6 months after onset |