Role of Macrophage in Inflammation Resolution in Periodontal Patients

Study Overview
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Brief Summary
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There is a growing body of evidence to suggest that the resolution of an inflammatory reaction is an active process. This process requiring signals that turn off early inflammatory stages, as neutrophil infiltration and promote resolution phase, as clearance of apoptotic cells. In this array,the relationship between neutrophils, monocytes, and macrophages is crucial for the concert process. Macrophages possess a plasticity of phenotype that explains their protective as well as potentially detrimental role in chronic inflammation and tissue injury. Such heterogeneity arises as macrophages differentiate from monocytes and are exposed to specific tissue- and hematopoietic cell-derived stimuli. It is important to be able to identify the different types of macrophages in this setting. Today's literature can distinguish between 3 main phenotypic groups of macrophages. The classically activated M1 macrophages, the alternatively activated-M2 macrophages and the resolution phase - rMs macrophages. The third phenotypic macrophage might play a crucial rule in the resolution phase, and is called the resolving macrophage. All of those pro-resolving lipid mediators promote the return to tissue homeostasis . The investigators hypothesis is that in periodontal patients the inflammatory process continues because the process does not reach the resolution phase, and in that milieu, the macrophage might be a key component. Within the overall concept of periodontal disease as a model for an inflammatory disease with an stoppable inflammatory reaction, the primary aim of the study is to check the macrophage characteristics in periodontal patients and to compare it to healthy people.

Official Title
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The Role of Macrophages in the Inflammatory Resolution Phase in Periodontal Patients

Conditions
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Periodontitis

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 years old and more, male & female generally healthy does not take medicine on a regular basis has a periodontal disease. control - without a periodontal disease no periodontal treatment for at list 6 months prior to his acceptance to the trail willing to donate 50 ml of blood twice during the trail understands the trail and gave a consent to participate Exclusion Criteria: pregnancy or nursing chronic use of alcohol systemic conditions - diseases, neoplastic lesions in the oral cavity

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| periodontitis<br>patients diagnosed with periodontal disease | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The macrophage phenotype (M1, M2, resolution phase macrophage) | we will try to specify the characteristics of the macrophage phenotype and to see whether there is a difference between periodontal patients and healthy ones. | 1 year |