Hemostasis Achievement Reflecting Inflammatory Status of the Pulp Based on Cytokine Levels: A Cohort Study.

Study Overview
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Brief Summary
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Assessment of pulp inflammation by hemostasis achievement after pulp exposure

Detailed Description
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The possibility has been raised of diagnosing pulpal pathological status using the cytokines that are released during the inflammation process in healthy as well as infected tooth pulp. Cytokines are small polypeptides secreted by leucocytes and other inflammatory cells and are known to play important roles in the intensity and duration of the immune response

Official Title
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Hemostasis Achievement Reflecting Inflammatory Status of the Pulp Based on Cytokine Levels: A Cohort Study

Conditions
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Hemorrhage

Intervention / Treatment
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* Diagnostic Test: ENzyme linked immunity-sorbet assays


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy children patients had not used any inflammatory medications primary molar teeth of these children were included based on the following clinical and radiographic criteria: no clinical symptoms or evidence of pulp degeneration (pain on percussion, history of swelling or sinus tract no spontaneous pain no radiographic signs of internal or external resorption, widened periodontal ligament space, or furcal/periapical radiolucency physiological root resorption of no more than one third the root length a deep caries lesion with a likelihood of pulp exposure during caries removal Exclusion Criteria: patients used any anti-inflammatory medication

Ages Eligible for Study
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Minimum Age: 5 Years
Maximum Age: 7 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| hemostasis achievement<br>in which hemostasis at the exposure site was achieved in five minutes were included in group A | Diagnostic Test: ENzyme linked immunity-sorbet assays<br>* Hemostasis achievement reflecting inflammatory status of the pulp based on cytokine levels<br>|
| No hemostasis achievement<br>in which hemostasis at the exposure site couldn't be achieved in five minutes where included in group B | Diagnostic Test: ENzyme linked immunity-sorbet assays<br>* Hemostasis achievement reflecting inflammatory status of the pulp based on cytokine levels<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measuring interleukin 2 (IL-2) | to measure cytokines levels after coronal pulpal removal | After 5 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measuring interleukin 6 (IL-6) and interleukin 8 (IL-8) | to measure cytokines levels after coronal pulpal removal | After 5 minutes | 
| Postoperative pain, Bifurcation/periapical radiolucency and Internal/external root resorption Or other pathological changes | To assess postoperative pain by verbal questioning and To observe presence or absence of radiolucency and root resorption or pathological condition | 3,6,9,12 months |