Effects of Dietary Fibre on Glucose Metabolism and Satiety

Study Overview
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Brief Summary
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The experimental model is a semi-acute study where the purpose of the study is to evaluate food factors related to colonially derived regulation of glucose metabolism (and related parameters) and satiety in healthy subjects.

Official Title
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Intrinsic Indigestible Carbohydrates as Means to Modulate Colonic Events, With Specific Focus on Metabolism and Satiety

Conditions
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Obesity, Metabolic Syndrome

Intervention / Treatment
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* Other: Test meal
* Other: Reference
* Other: Priming
* Other: No priming


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Normal fasting blood glucose, BMI 19-25 kg/m2 Exclusion Criteria: Gastrointestinal diseases or food allergies e.g. lactose-, gluten intolerance, metabolic disorders e.g. diabetes, tobacco/snuff users. Antibiotic or probiotic usage within two weeks, and during study. Vegetarians.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: + ind. CHO + prim<br>Test meal: ate intrinsic indigestible carbohydrates 3 days prior to measurements of variables (priming) | Other: Test meal<br> <br> Other: Priming<br>* The reference or test meal was eaten 3 days prior to measurements of variables.<br>|
| Experimental: - ind. CHO + prim<br>Reference:ate no intrinsic indigestible carbohydrates 3 days prior to measurements of variables (priming) | Other: Reference<br> <br> Other: Priming<br>* The reference or test meal was eaten 3 days prior to measurements of variables.<br>|
| Experimental: + ind. CHO - prim<br>Test meal: ate intrinsic indigestible carbohydrates 1 day prior to measurements of variables (no priming) | Other: Test meal<br> <br> Other: No priming<br>* The reference or test meal was eaten 1 days prior to measurements of variables.<br>|
| Experimental: - ind. CHO - prim<br>Reference: ate no indigestible carbohydrates the day prior to measurements of variables (no priming). | Other: Reference<br> <br> Other: No priming<br>* The reference or test meal was eaten 1 days prior to measurements of variables.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood glucose | The test products are both ingested one day and three days prior to measurements of variables. | Postprandial 0-180 min after breakfast | 
| Insulin | The test products are both ingested one day and three days prior to measurements of variables. | Postprandial 0-180 min after breakfast | 
| Appetite hormones | The test products are both ingested one day and three days prior to measurements of variables. | Postprandial 0-180 min after breakfast | 
| Markers of colonic fermentation and inflammation | The test products are both ingested one day and three days prior to measurements of variables. | Postprandial 0-180 min after breakfast | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Subjective satiety | The measures of hunger, satiety, and desire to eat with the use of a VAS were determined every hour throughout the postprandial testing sessions. | Postprandial 0-180 min after breakfast |