Carotenoid Response to the Intake of Vegetables and Fruits

Study Overview
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Brief Summary
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The purpose of this study is to validate the non-invasive tool, resonance Raman spectroscopy for assessing vegetable and fruit consumption.

Detailed Description
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This study will validate the use of resonance Raman spectroscopy to assess vegetable and fruit intake by performing a controlled feeding study during which volunteers will consume a provided diet with known amounts of carotenoids in the form of fruits and vegetables.

Official Title
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Skin Carotenoid Response to Intake of Vegetables and Fruits Recommended by the Dietary Guidelines

Conditions
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Resonance Raman Spectroscopy for Dietary Carotenoid Intake

Intervention / Treatment
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* Other: Carotenoid Depletion
* Other: Carotenoid Repletion
* Other: Carotenoid Depletion
* Other: Natural Repletion


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Men and women aged 18 - 65 years Weight stable (±5 lbs) for at least 2 months prior to the start of the study Body mass index (BMI) between 19-30 kg/m2 Willing to refrain from the use of nicotine and dietary supplements for the duration of the study Willing to maintain baseline weight and physical activity levels Exclusion Criteria: BMI greater than 30. Gastrointestinal disorders, disease, or medication that affects carotenoid absorption or storage Current smoker Allergies or intolerances to foods to be utilized in the study Consumption of greater than an average of 1 alcoholic drink/day for women and 2/day for men, use of nutritional/sports supplements (if unwilling to discontinue during the study) Pregnancy or lactating

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Skin Carotenoids<br>Skin carotenoids will be measured under conditions of depletion or repletion | Other: Carotenoid Depletion<br>* Depletion of carotenoids using a restricted food list<br>Other: Carotenoid Repletion<br>* Carotenoid repletion with provided diet based on dietary guidelines.<br>Other: Carotenoid Depletion<br>* Carotenoid depletion using a restricted food list.<br>Other: Natural Repletion<br>* Resumption of usual personal diet regimen. No restrictions.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serum and skin levels of carotenoids in adults fed a high carotenoid diet |  | 28 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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MyPlate, resonance Raman spectroscopy, Dietary Guidelines, fruits, biomarkers, carotenoids, vegetables