PACE4 in Thyroid Cancer

Study Overview
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Brief Summary
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The investigation of thyroid nodules is limited by the fact that up to 49% of the fine needle aspirations (FNA) performed are of indeterminate cytological signification. Moreover, no fully reliable molecular marker for thyroid cancer have been described. The goal of this project is to study the expression of a specific kind of protein convertase in benign and in malignant thyroid nodules to determine its potential as a biomarker.

Detailed Description
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Patients that underwent thyroidectomy at the CIUSSS de l'Estrie-CHUS will be recruited and classified according to the pathological diagnosis. An immunohistochemistry technique, developed and calibrated for the specific protein convertase studied, will be undertaken. Results will be interpreted by a specialized pathologist.

Official Title
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Evaluation of PACE4 Isoforms as Biomarkers in Thyroid Cancer

Conditions
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Thyroid Nodule

Intervention / Treatment
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* Diagnostic Test: Immunohistochemistry


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Men of women, aged 18-100 y.-o. History of subtotal or total thyroidectomy at the Centre intégré universitaire de santé et de services sociaux de l'Estrie - Centre hospitalier universitaire de Sherbrooke (CIUSSS de l'Estrie - CHUS) between January 2010 and May 2016. Exclusion Criteria: Refusal to participate to the study. Unavailability of the formalin-fixed, paraffin-embedded tissues. Uncertain pathological diagnosis.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 100 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Papillary carcinoma, classical variant<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Follicular carcinoma<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Colloid nodule<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Hyperplastic nodule<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Adenomatoid nodule<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Follicular adenoma<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Papillary carcinoma, follicular variant<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Medullary carcinoma<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|
| Lymphocytic thyroiditis<br> | Diagnostic Test: Immunohistochemistry<br>* Immunohistochemistry procedure on the formalin-fixed, paraffin-embedded tissue.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Expression pattern of the studied protein convertase | Horseradish peroxidase staining with specific antibodies. | Trough study completion, an average of 1 year after the patients have been operated. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Thyroid Neoplasm, Thyroid Cancer, Bethesda Classification, Biomarkers, Protein convertase