Consumption of Dairy Yogurt Enhanced Immune Function

Study Overview
=================
Brief Summary
-----------------
To investigate the impact of consumption of dairy yogurt with rhamnogalacturonan (RG) in Korean citrus Hallabong peel polysaccharide on Natural Killer (NK) cell activity and circulating cytokine levels.

Detailed Description
-----------------
A randomized, double-blind, placebo-controlled study was conducted on 120 nondiabetic and nonobese subjects. Over an 8 week period, the test group consumed one pack (150ml) of dairy yogurt with 50mg probiotics and 100mg Korean citrus Hallabong peel polysaccharide (60% RG, 20% mono- and di-saccharide, 5% polyphenol) each day, whereas the placebo group consumed the same product without Korean citrus Hallabong peel polysaccharide. NK cell activities (%) were measured based on the ratios of effector cells (E) (peripheral blood mononuclear cell, PBMC) from each participant to Target cell (T) (K562 cells) as 10:1, 5:1, 2.5:1 or 1.25:1.

Official Title
-----------------
Consumption of Dairy Yogurt With Rhamnogalacturonan in Korean Citrus Hallabong Peel Polysaccharide Enhanced Immune Function and Attenuated Inflammatory Response

Conditions
-----------------
Healthy Volunteers

Intervention / Treatment
-----------------
* Dietary Supplement: RG
* Dietary Supplement: Placebo


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: The levels of white blood cells under 8x10^3/μL males and females 30-69 years old nondiabetic and nonobese subjects able to give informed consent Exclusion Criteria: consumption of any probiotic products within 1 month before screening unstable body weight (body-weight change > 2 kg within 3 months before screening) diabetes history/presence of significant metabolic disease pregnancy or breast-feeding acute or chronic infection liver disease, kidney disease, gastrointestinal disease, or any other acute or chronic disease requiring treatment subjects taking any drugs or supplements

Ages Eligible for Study
-----------------
Minimum Age: 30 Years
Maximum Age: 69 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: test group<br>Dietary Supplement: RG consumed one pack (150ml) of dairy yogurt containing RG (100mg Korean citrus Hallabong peel polysaccharide) per day for 8 weeks | Dietary Supplement: RG<br>* The 100mg Korean citrus Hallabong peel polysaccharide contained 60mg rhamnogalacturonan (RG), 20mg mono- and di-saccharide, 5mg polyphenol, 5mg ash and 10mg moisture<br>|
| Placebo Comparator: placebo<br>Dietary Supplement: placebo consumed one pack (150ml) of dairy yogurt without RG per day for 8 weeks | Dietary Supplement: Placebo<br>* Same product without RG<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| serum cytokine concentrations | Primary outcome will be tested before and after intervention | change from baseline in RG at 8 weeks | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
rhamnogalacturonan, Natural killer cell activity, interleukin-12, interferon-gamma, immune function, inflammatory