Muscular Force Generation in Microgravity

Study Overview
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Brief Summary
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The aim of this study is to evaluate the applicability of high-load resistance training as a countermeasure for long term space flights, the current project has two scientific goals. The main objective is to perform a deeper examination of muscle function and MVC in weightlessness (parabolic flight). The secondary objective is to gather further insight into the underlying mechanisms and structures based on investigations targeting the neuromuscular and musculotendinous systems and their interaction.

Detailed Description
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The findings from water immersion indicated that some motor tasks, such as plantarflexion (25), but not others, such as the leg-press (7), appear to be affected. Thus, it would be relevant to test a number of motor tasks and body regions. On the other hand, parabolic flight requires that methodologies can be performed in a quick efficient fashion. Hence, we propose examining ankle plantarflexion, leg-press and grip-force. The primary hypothesis is that plantarflexion MVC will be reduced in the microgravity phase of parabolic flight. As secondary parameters will be assessed parameters of muscle function that can be easily implemented in parabolic flight, such as electromyography and ultrasound measurements of the musculotendinous unit. To assess the muscular system more deeply, for plantarflexion superimposed twitches will be performed (16) during MVC and at rest. An additional experimental condition placing foam (unstable surface; standard size) under the foot during plantarflexion will be included. This approach increases the challenge for the neuromuscular control system and, under such conditions, MVC is reduced (29). These secondary investigations, which do not prohibitively complicate the practical implementation of the project, will help to understand what is happening in the musculature should we indeed find impediments of muscle force generation in microgravity. The aim of this study is to evaluate the applicability of high-load resistance training as a countermeasure for long term space flights, the current project has two scientific goals. The main objective is to perform a deeper examination of muscle function and MVC in weightlessness (parabolic flight). The secondary objective is to gather further insight into the underlying mechanisms and structures based on investigations targeting the neuromuscular and musculotendinous systems and their interaction. The main judgment criteria is the MVC tested during three different tasks: (1) leg press, (2) plantarflexion and (3) grip strength. As secondary criteria, we will assess parameters of muscle function that can be easily implemented in parabolic flight: Rate of force development Electromyographic activity Ultrasound measure: fascicle length and pennation angle of the gastrocnemius medialis muscle Superimposed twitch torque (M-waves at rest and V-waves in MVC conditions) Maximum voluntary activation capacity (%VA): %VA = (1-[torque superimposed twitch/torque resting twitch])*100%. The primary hypothesis is that plantarflexion and knee extension force generation capacity will be reduced, but not grip-force, in the microgravity phase of parabolic flight.

Official Title
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Muscular Force Generation in Microgravity (ForceGeneration)

Conditions
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Maximum Voluntary Contraction

Intervention / Treatment
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* Other: examination of muscle function and MVC in weightlessness


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy volunteers (men or women) Aged from 21 to 65 Affiliated to a Social Security system and, for non-French resident, holding a European Health Insurance Card (EHIC) Who accepted to take part in the study Who have given their written stated consent Who have their right leg as their preferred leg Who has passed a medical examination similar to a standard aviation medical examination for private pilot aptitude (JAR FCL3 Class 2 medical examination). There will be no additional test performed for subject selection. Subjects will be staff member of the team or of other teams participating in the parabolic flight campaign Exclusion Criteria: Person who took part in a previous biomedical research protocol, of which exclusion period is not terminated Person with orthopaedic disorders in the upper or lower limbs Person with neurological disorders Pregnant women

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: high-load resistance training for long term space flights<br>Short duration electrical will be delivered and the resulting EMG will be recorded. Intensity of electrical stimulation will be progressively increased until we observed a maximal EMG response. Finally an ultrasound probe (similar to that used for prenatal diagnostic imaging) will be fixed behind your calf in order to visualize muscle. | Other: examination of muscle function and MVC in weightlessness<br>* MVC tested during three different tasks: (1) leg press, (2) plantarflexion and (3) grip strength. parameters of muscle function that can be easily implemented in parabolic flight: Rate of force development Electromyographic activity Ultrasound measure: fascicle length and pennation angle of the gastrocnemius medialis muscle Superimposed twitch torque (M-waves at rest and V-waves in MVC conditions) Maximum voluntary activation capacity (%VA): %VA = (1-[torque superimposed twitch/torque resting twitch])*100%.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| maximum voluntary contraction (MVC) | tested during three different tasks: (1) leg press, (2) plantarflexion and (3) grip strength. | baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| parameters of muscle function, Rate of force development |  | baseline | 
| parameters of muscle function, Electromyographic activity |  | baseline | 
| parameters of muscle function, Ultrasound measure: fascicle length |  | baseline | 
| parameters of muscle function, Ultrasound measure: pennation angle of the gastrocnemius medialis muscle |  | baseline | 
| parameters of muscle function, Superimposed twitch torque (M-waves at rest and V-waves in MVC conditions) |  | baseline | 
| parameters of muscle function, Maximum voluntary activation capacity (%VA): %VA = (1-[torque superimposed twitch/torque resting twitch])*100%. |  | baseline |