The Effect of Leukocyte and Platelet-rich Fibrin With Antimicrobial Photodynamic Therapy in Aggressive Periodontitis

Study Overview
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Brief Summary
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Antimicrobial photodynamic therapy (aPDT) is associated with photosensitizing agents which promote the generation of free radicals and singlet oxygen, which are cytotoxic to certain bacteria. Leukocyte and platelet-rich fibrin (L-PRF) has been used extensively in the treatment of intrabony defects and achieved excellent results. It acts as an immune regulation node with inflammation control abilities, including a slow continuous release of growth factors which stimulates periodontal regeneration. The aim of this study is to evaluate the adjunctive effects of aPDT with and without L-PRF in aggressive periodontitis patients.

Official Title
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The Effect of Leukocyte and Platelet-rich Fibrin With Antimicrobial Photodynamic Therapy in Treatment of Aggressive Periodontitis Patients: A Randomized Controlled Clinical Trial

Conditions
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L-PRF, Photodynamic Therapy, Aggressive Periodontitis

Intervention / Treatment
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* Procedure: L-PRF with antimicrobial photodynamic therapy
* Procedure: antimicrobial photodynamic therapy without L-PRF


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: systemically healthy non- smoker, not received any periodontal treatment within the last 6 months, no antibiotics, anti- inflammatory drugs or any other medication taken within the last 6 months Exclusion Criteria: any systemic disease that might influence the prognosis of periodontal disease and outcome of the treatment, any medical condition that required antibiotic prophylaxis before the treatment, smoking, pregnancy and lactation, ingestion of antibiotics, anti-inflammatory drugs or any other medication during the study period, any physical limitations or restrictions that might preclude normal oral hygiene procedures.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: L-PRF with antimicrobial photodynamic therapy<br>Application of L-PRF with antimicrobial photodynamic therapy in the defect | Procedure: L-PRF with antimicrobial photodynamic therapy<br>* Application of L-PRF with antimicrobial photodynamic therapy in the defect<br>|
| Active Comparator: antimicrobial photodynamic therapy<br>Application antimicrobial photodynamic therapy in the defect | Procedure: antimicrobial photodynamic therapy without L-PRF<br>* application of antimicrobial photodynamic therapy without L-PRF in the defect<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Clinical evaluation and measurements of the defect in millimeters | Changes in clinical measurements in millimeters after application of antimicrobial photodynamic therapy with and without L-PRF | 9 months | 
| Radiographic evaluation and measurements of the defect in millimeters | Changes in bone formation in millimeters after application of antimicrobial photodynamic therapy with and without L-PRF | 9 months |