Elevated Plasma Levels of TIMP-1 in Patients With Rotator Cuff Tear

Study Overview
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Brief Summary
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The aim of the study was to investigate the levels of matrixmetalloproteinases and their inhibitors in blood samples from patients with rotator cuff tears and compare with blood samples from healthy matched controls.

Detailed Description
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Background: Extracellular matrix remodeling is altered in rotator cuff tears, partly due to altered expression of matrix metalloproteinases (MMPs) and their inhibitors. It is unclear if this altered expression can be traced as changes in plasma protein levels. We measured the plasma level of MMPs and their tissue inhibitors (TIMPs) in patients with rotator cuff tears. Material and methods: Blood samples were collected from 17 patients, median 61 (range 39-77) years, with sonographically verified rotator cuff tears (partial- or full-thickness). These were compared with 16 gender and age matched control persons with sonographically intact rotator cuffs. Plasma levels of MMPs and TIMPs were measured simultaneously using Luminex technology.

Official Title
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Elevated Plasma Levels of TIMP-1 in Patients With Rotator Cuff Tear

Conditions
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Rotator Cuff Tear

Intervention / Treatment
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* Procedure: Blood sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - degenerative rotator cuff tear verified by ultrasound Exclusion Criteria: systemic joint disease such as rheumatoid arthritis a fracture non-union dupuytrens disease frozen shoulder tendinosis or rupture of any other tendons than in the rotator cuff disorders of the spine such as disc disease,idiopathic scoliosis, spondylitis cerebral or cardiovascular disease during the past year abdominal or bowel disease surgery or trauma during the past year any infection during the last month malignancy treatment for the last month with medications that may affect MMPs or TIMPs (tetracycline, bisphosphonates, anti-inflammatory drugs, statins) vigorous physical activity during the last 24 hours inability to understand written and spoken Swedish
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients with rotator cuff tears<br>Patients with ultrasound verified rotator cuff tears | Procedure: Blood sampling<br>* Four mL venous blood samples were collected from all study participants after inclusion<br>|
| Healthy controls<br>Age and gender matched controls with ultrasound verified intact rotator cuff | Procedure: Blood sampling<br>* Four mL venous blood samples were collected from all study participants after inclusion<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Level of MMP and TIMP in plasma | Four mL venous blood samples were collected from all study participants after inclusion, and centrifuged to plasma that was stored at -70 degrees until the analysis. | Measaured at one time point | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Rotator cuff tear, matrix metalloproteinase, tissue inhibitor of matrix metalloproteinase, partial-thickness tear, full-thickness tear, plasma.