Assessment of Candidate Protein Expression in Breast Cancer Specimens

Study Overview
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Brief Summary
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RATIONALE: Studying the genes expressed in samples of tumor tissue from patients with cancer may help doctors identify biomarkers related to cancer. PURPOSE: This research study is looking at tumor tissue samples from patients who have undergone surgery for breast cancer.

Detailed Description
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OBJECTIVES: To determine if candidate genes display differential expression between normal and cancer breast tissues. OUTLINE: Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.

Official Title
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Assessment of Candidate Protein Expression in Human Breast Cancer Specimens

Conditions
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Breast Cancer

Intervention / Treatment
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* Genetic: gene expression analysis
* Other: immunohistochemistry staining method
* Other: laboratory biomarker analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: DISEASE CHARACTERISTICS: Diagnosis of breast cancer Tissue samples available Samples are selected based on presence or absence of tumor receptors, (i.e.; estrogen, progesterone, and HER2 receptors) and grouped into following 4 categories: Estrogen receptor (ER)-positive/progesterone receptor (PR)-positive/HER2-negative ER-positive/PR-positive/ HER2-positive by FISH ER-negative/PR-negative/HER2-positive ER-negative/PR-negative/HER2-negative(triple negative or basal-type cancers) PATIENT CHARACTERISTICS: Menopausal status not specified PRIOR CONCURRENT THERAPY: Not specified Exclusion Criteria: There are no exclusion criteria in this protocol
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Genetic: gene expression analysis|Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.|
|Other: immunohistochemistry staining method|Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.|
|Other: laboratory biomarker analysis|Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Differential expression of candidate genes between normal and cancer breast tissues | Approximately 5 slides of each tumor will be collected to provide sufficient samples for replicate staining, if necessary, as well as a staining for a positive control | non-applicable | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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breast cancer, estrogen receptor-negative breast cancer, estrogen receptor-positive breast cancer, progesterone receptor-negative breast cancer, progesterone receptor-positive breast cancer, triple-negative breast cancer, HER2-negative breast cancer, HER2-positive breast cancer