Cell-free Fetal DNA Concentration in Cases of Abnormal Placental Invasion .

Study Overview
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Brief Summary
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Cell free fetal DNA might predict abnormal placental invasion

Detailed Description
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This study aims to evaluate whether measurement of cell-free fetal DNA in the maternal plasma can be predictive of abnormal invasion in cases of placenta previa.

Official Title
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Predictive Value of Cell-free Fetal DNA Concentration in the Maternal Plasma for Abnormal Placental Invasion in Cases of Placenta Previa.

Conditions
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Placenta Accreta, Placenta Percreta, Placenta Increta, Placenta Previa

Intervention / Treatment
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* Diagnostic Test: Maternal plasma cell free fetal DNA levels


Participation Criteria
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Eligibility Criteria
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Inclusion criteria: Maternal age range from 20-40 years old. Parity: para 1- para 5. Body mass index range from 18-25 kg/m2. Singleton pregnancy carrying male fetuses. Gestational age range from 28-34 weeks. Exclusion criteria: Multifetal pregnancy. Hypertension, preterm labor and intrauterine growth restriction. Patients taking a tocolytic agent or those with uterine bleeding at or after blood sampling. Since these complications may increase the level of cell-free fetal DNA. Accidental hemorrhage.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Study group<br>Maternal plasma cell free fetal DNA levels will be measured in 25 women with one or more previous cesarean section and placenta previa anterior only or with ultrasound finding suggestive of placental adhesion or invasion (accreta , increta or percreta). | Diagnostic Test: Maternal plasma cell free fetal DNA levels<br>* Measurement Maternal plasma cell free fetal DNA levels in both groups (study and control groups)<br>|
| Control group<br>Maternal plasma cell free fetal DNA levels will be measured in 25 matched control with normally situated placenta without ultrasound finding suggestive of placental adhesion or invasion. | Diagnostic Test: Maternal plasma cell free fetal DNA levels<br>* Measurement Maternal plasma cell free fetal DNA levels in both groups (study and control groups)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Predictive value of maternal plasma cell free DNA | Prediction of abnormal placental invasion in cases of placenta previa. Primary aim: Is to evaluate whether measurement of cell-free fetal DNA in the maternal plasma can be predictive of abnormal placental invasion in cases of placenta previa. Secondary aim: To decrease maternal morbidity and mortality by early prenatal diagnosis of abnormal placental invasion in placenta previa | 4 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| maternal morbidity | maternal morbidity | 4 months |