Quantification of Microbiota in Health and Diseases

Study Overview
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Brief Summary
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Microbiota consists of trillions of microorganisms located in all the biological cavity. The relationships between individual health status and microbiota are based on a sort of mutual cooperation. The balance of the microbiota can be compromised by various factors such as environmental, psycho-physical stress, malnutrition (over or low), breastfeeding, chronic diseases, oncology and pharmacological treatments (especially antibiotics and chemotherapics). The impact of dysbiosis on today's society affects over 70% of the world population

Detailed Description
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Intestinal microbiota is defined as the strongest modulator of the human genome and it is considered the second brain of our organism for its bi-directional communication with the central nervous system. Microbiota consists of trillions of microorganisms located in all the biological cavity, In the intestine it is distributed in ecological niches having multiple metabolic activities which influence individual health. Microbiota acts as barrier against pathogens, regulating the absorption of nutrients, the production of energy and the development of the immune system4. Intestinal bacteria are generally divided into 4 Phylum: Firmicutes, Actinobacteria, Proteobacteria and Bacteriodetes, in which their proportion shows a correlation with individuals health status. When in equilibrium among them, these bacteria are essential for the good health of the digestive tract, the good function of immune system and for brain performace. The relationships between individual health status and microbiota are based on a sort of mutual cooperation. The balance of the microbiota can be compromised by various factors such as environmental, psycho-physical stress, malnutrition (over or low), breastfeeding, chronic diseases, oncology and pharmacological treatments (especially antibiotics and chemotherapics). This disequilibrium leads to altered capability of bacteria to properly extract calories from food affecting the trend of many pathophisilogical status such as obesity, allergic states, inflammatory bowel syndromes cardiovascular diseases, metabolic, oncological and neurodegenerative diseases. The impact of dysbiosis on today's society affects over 70% of the world population

Official Title
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Quantification of Microbiota in Health and Diseases

Conditions
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Disease

Intervention / Treatment
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* Diagnostic Test: quantification of microbiota


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: all patients will be enclosed in each group in agreement with the characteristics of the groups Exclusion Criteria: Patients who will not sign the informed consent

Ages Eligible for Study
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Minimum Age: 4 Years
Maximum Age: 90 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Control<br>Healthy people of both sex | Diagnostic Test: quantification of microbiota<br>* For each group a sample of biological sample will be take and will be analyzed using the n-counter flex platform in order to quantify disbiosis<br>|
| quantification of microbiota in Brain<br>Patients of both sex with neural disorders and diseases | Diagnostic Test: quantification of microbiota<br>* For each group a sample of biological sample will be take and will be analyzed using the n-counter flex platform in order to quantify disbiosis<br>|
| quantification of microbiota in GI<br>Patients of both sex with gastrointestinal diseases | Diagnostic Test: quantification of microbiota<br>* For each group a sample of biological sample will be take and will be analyzed using the n-counter flex platform in order to quantify disbiosis<br>|
| quantification of microbiota in Lung<br>Patients of both sex with Respiratory diseases | Diagnostic Test: quantification of microbiota<br>* For each group a sample of biological sample will be take and will be analyzed using the n-counter flex platform in order to quantify disbiosis<br>|
| quantification of microbiota Metabolic<br>Patients of both sex with Metabolic Diseases | Diagnostic Test: quantification of microbiota<br>* For each group a sample of biological sample will be take and will be analyzed using the n-counter flex platform in order to quantify disbiosis<br>|
| quantification of microbiota in UG<br>Patients of both sex with Uro-genital Diseases | Diagnostic Test: quantification of microbiota<br>* For each group a sample of biological sample will be take and will be analyzed using the n-counter flex platform in order to quantify disbiosis<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determination of microbiota percentage | we will determine the difference of microbiota percentage in diseases-groups respect to control-group | 12 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Determination of antibiotic resistance gene | it will be evaluate the presence of antibiotic resistance gene in diseases-group respect to control-group | 12 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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bacterial DNA, RNA16S gene