The Impact of Enteral Versus Oral Protein Feeding on Muscle Protein Synthesis in Healthy Young Males and Females

Study Overview
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Brief Summary
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The present study will seek to quantify the muscle protein synthetic response to a protein beverage consumed orally or through a nasogastric tube in healthy, young individuals.

Detailed Description
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Thirty healthy, young volunteers will receive a stable isotope tracer infusion (8.5h) combined with repeated blood and muscle sampling, in order to measure muscle protein synthesis rate in the postabsorptive state, and following oral placebo ingestion (n=10), oral protein ingestion (n=10), or enteral protein administration (n=10).

Official Title
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The Impact of Enteral Versus Oral Protein Feeding on Muscle Protein Synthesis in Healthy Young Males and Females

Conditions
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Healthy

Intervention / Treatment
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* Other: Oral Placebo
* Other: Oral Protein
* Other: Enteral Protein


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18-40 years of age Body mass index between 18.5 and 30 Exclusion Criteria: Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes) Any diagnosed cardiovascular disease Elevated blood pressure at the time of screening. (An average systolic blood pressure reading of ≥140 mmHg over two or more measurements and an average diastolic blood pressure of ≥90 mmHg over two or more measurements) Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism). This excludes oral contraceptives and contraceptive devices. A personal or family history of epilepsy, seizures or schizophrenia. Presence of an ulcer in the stomach or gut and/or strong history of indigestion Known pre-existing liver disease/condition Any known disorders in muscle metabolism Regular use of nutritional supplements Allergy to lidocaine Allergy to milk Current paracetamol use (i.e. use of paracetamol more than once a week) Pregnancy

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Oral Placebo<br>Placebo drink | Other: Oral Placebo<br>* A placebo beverage will be consumed orally<br>|
| Active Comparator: Oral Protein<br>Protein drink, ingested orally | Other: Oral Protein<br>* A protein beverage will be consumed orally<br>|
| Active Comparator: Enteral Protein<br>Protein drink, administered via enteral tube | Other: Enteral Protein<br>* A protein beverage will be consumed via a naso-gastric feeding tube<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Postprandial muscle protein synthesis | Muscle protein synthesis rate (FSR) following ingestion of a placebo or protein supplement | 5 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Postabsorptive muscle protein synthesis | Muscle protein synthesis rate (FSR, in %/h) during a 2 hour fasting period | 2 hours | 
| Whole-body protein synthesis | Whole-body protein synthesis, measured using a D5 phenylalanine and D2 tyrosine infusion (umol Phenylalanine/kg/h) | 7 hours | 
| Whole-body protein breakdown | Whole-body protein breakdown, measured using a D5 phenylalanine and D2 tyrosine infusion (umol Phenylalanine/kg/h) | 7 hours | 
| Whole-body protein oxidation | Whole-body protein oxidation, measured using a D5 phenylalanine and D2 tyrosine infusion (umol Phenylalanine/kg/h) | 7 hours | 
| Whole-body protein net balance | Whole-body protein net balance, measured using a D5 phenylalanine and D2 tyrosine infusion (umol Phenylalanine/kg/h) | 7 hours | 
| Gastric emptying rate | Gastric emptying rate following ingestion of a placebo or protein drink | 5 hours | 
| Blood glucose concentration | Blood glucose concentration in the postabsorptive and postprandial phase following placebo/protein ingestion | 8.5 hours | 
| Serum insulin concentration | Serum insulin concentration in the postabsorptive and postprandial phase following placebo/protein ingestion | 8.5 hours |