Protein Requirements in Resistance Trained Females

Study Overview
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Brief Summary
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In this study, the investigators will use the minimally invasive indicator amino acid oxidation (IAAO) technique to determine protein requirements in weight-trained males. It is hypothesized that the present study will show that protein requirements for weight-trained females are i) greater than the current RDA for non-active individual's comparable estimates, and ii) greater than existing nitrogen balance-based estimates for weight-training individuals

Detailed Description
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Provided that energy needs are met, the adequate ingestion of dietary amino acids is the most critical nutritional factor to support the optimal remodeling and deposition of lean body mass in individuals of all ages. Current recommendations according to the World Health Organization/Food and Agricultural Organization suggest that daily protein requirements in healthy, non-active adults are 0.8 g/kg/day. However, of primary interest in the present study is the impact that exercise has on the nutritional requirement for dietary amino acids in highly active adults - e.g., individuals performing weight training. Protein requirements for individuals who participate in strength-based exercise training have been suggested to range from 1.2-1.7g protein/kg/day (Rodriguez et al., 2009; Tarnopolsky, 1992; Phillips et al., 2007), which equates to a 50-112% increase from the current RDA. The increased requirement in this population may reflect the requirement for protein to repair/rebuild lean tissues by promoting anabolism (Phillips, 2004). Nutritional requirements for dietary amino acids in adults (both active and non-active) have traditionally been determined utilizing the antiquated and often erroneous nitrogen balance technique (Costaneda et al., 1995; Campbell et al., 1984), which is prone to underestimating protein requirements and therefore provides challenges to making accurate nutritional recommendations (Humayun, Elango, Ball, and Pencharz, 2007). This observation that nitrogen balance underestimates protein requirements in non-active individuals could suggest that protein requirements are much greater than the current WHO/FAO recommendation of 0.8 g/kg/day which was evaluated using the nitrogen balance technique (Rand, Pellett and Young, 2003). As a result, there is a need to re-evaluate recommendations utilizing advanced stable isotope methodology in order to characterize how dietary amino acid needs may be modulated by physical activity. Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than WHO/FAO guidelines based on nitrogen balance data (Humayun, Elango, Ball, and Pencharz, 2007). Furthermore, resistance training has been reported to increase (according to nitrogen balance methodology) protein requirements by up to 75% Tarnopolsky et al., 1992). Therefore, in this study, the investigators will use the IAAO technique to determine protein requirements in weight-trained males. It is hypothesized that the present study will show that protein requirements for weight-trained females are i) greater than the current RDA for non-active individual's comparable estimates, and ii) greater than existing nitrogen balance-based estimates for weight-training individuals.

Official Title
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Protein Requirements in Resistance Trained Females

Conditions
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Healthy

Intervention / Treatment
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* Other: Protein intake (dose)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy female weight-trained individuals that have trained consistently for >1 year complete a physical activity readiness questionnaire (PAR-Q+) Train each muscle group (i.e. chest, back, legs) at least twice a week Meets strength relative to body weight GUIDELINES adapted from Morton et al., 2016; Chilibeck et al., 1997 (sex specific). Bench Press: body weight (kg)*0.7 Leg Press: body weight (kg)*2.3 Exclusion Criteria: sedentary for greater than 1 month in the last 6 months prior to the study non-weight stable for the past month smoker Illicit drug use (e.g. growth hormone, testosterone, etc.) Individual plans to increase or decrease body mass in the next 3 months Habitually ingests greater than or equal to 3g protein kg/bw/day Use of supplements such as creatine and beta-alanine in the last 30 days. Hormone-based birth control. Hormonal fluctuations associated with the menstrual cycle have been reported to alter protein metabolism during exercise (Hamadeh, Devries, and Tarnopolsky, 2005) For this reason, the following exclusion criteria will be applied to female participants only: use of birth control and discontinued use within last 3 months Inability to adhere to any of the protocol guidelines

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Healthy females<br>All participants receive the intervention: Protein intake (dose). This consists of varying levels of dietary protein intakes, in the form of crystalline amino acids, ranging from 0.2-3.0 g/kg/d | Other: Protein intake (dose)<br>* Protein intake, in the form of crystalline amino acids, will range between 0.2-3.0 g/kg/d<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Phenylalanine oxidation | In µmol/kg/h, the product of F13CO2 and VCO2. F13CO2 is determined via breath enrichment analysis of 13CO2 after 1-13C phenylalanine ingestion, and VCO2 is determined via indirect calorimetry | Through study completion: an average of 6 metabolic trials (days), each separated by approximately 1 week | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Phenylalanine rate of appearance | In µmol/kg/h; calculated from urine analysis of1-13C Phe enrichment | Through study completion: an average of 6 metabolic trials (days), each separated by approximately 1 week | 
| Net protein balance | In µmol/kg/h; calculated as the difference between whole body net protein synthesis and protein breakdown | Through study completion: an average of 6 metabolic trials (days), each separated by approximately 1 week |