Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men

Study Overview
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Brief Summary
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The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.

Detailed Description
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This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.

Official Title
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The Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men: a Randomized Controlled Trial

Conditions
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Skeletal Muscle, Extracellular Matrix, Amino Acids Metabolism, Exercise, Dietary Supplements

Intervention / Treatment
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* Dietary Supplement: Essential Amino Acid
* Dietary Supplement: Collagen Peptide
* Dietary Supplement: Maltodextrin Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Can fluently read and write in English Male 18 - 35 years Exclusion Criteria: Diabetes, cardiovascular disease, or current pharmaceutical therapy Medical conditions impacting ability to undertake strenuous physical activity Lower body resistance training in the previous 6 months BMI > 30 Currently smoking with cigarettes Allergy to any study supplements Currently taking any protein, amino acid, or collagen supplement Participate in greater than 2 hours of weekly vigorous exercise

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: - Double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid)
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Essential Amino Acid<br>Participants will consume 1 dose of: 15 g crystalline essential amino acid supplement (Pure Encapsulation Essential Aminos 180) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water | Dietary Supplement: Essential Amino Acid<br>* Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement<br>|
| Active Comparator: Collagen Peptide<br>Participants will consume 1 dose of: 15g collagen peptide (Gelita TENDOFORTE) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water | Dietary Supplement: Collagen Peptide<br>* Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement<br>|
| Placebo Comparator: Maltodextrin Placebo<br>Participants will consume 1 dose of:15 g maltodextrin (Canadian Protein Maltodextrin) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water | Dietary Supplement: Maltodextrin Placebo<br>* Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis | Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. | 0 - 4 hours post-exercise | 
| Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis | Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. | 0 - 2 hours post-exercise | 
| Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis | Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. | 2 - 4 hours post-exercise | 
| Fractional synthetic rate (change over time) Muscle Collagen Synthesis | Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. | 0 - 4 hours post-exercise | 
| Fractional synthetic rate (change over time) Muscle Collagen Synthesis | Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. | 0 - 2 hours post-exercise | 
| Fractional synthetic rate (change over time) Muscle Collagen Synthesis | Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. | 2 - 4 hours post-exercise | 
| Pro-Collagen Type I N-Propeptide (PINP) | Osteoblastic marker of bone collagen synthesis | 1 hour prior to exercise | 
| Pro-Collagen Type I N-Propeptide (PINP) | Osteoblastic marker of bone collagen synthesis | 4 hours post exercise | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma Amino Acids Response to Supplementation | Amino acid plasma concentrations | 0 - 4 hours post-exercise | 
| Muscle mechanistic target of rapamycin Pathway Activation | Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt)) | 0 - 4 hours post-exercise | 
| PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV) | Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis | 0 - 4 hours post-exercise | 
| Pro-Collagen Type III N-Propeptide (PIIINP) | Marker of collagen synthesis | 1 hour prior to exercise | 
| Pro-Collagen Type III N-Propeptide (PIIINP) | Marker of collagen synthesis | 4 hours post exercise | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Muscle Collagen Turnover, Myofibrillar Protein Synthesis, Extracellular Matrix, Anabolism Signaling, Resistance Exercise, Skeletal Muscle, Bone Turnover