Homeostatic and Non-homeostatic Processing of Food Cues in Anorexia Nervosa

Study Overview
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Brief Summary
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The goal of the present study is to investigate metabolic gut-brain signaling and the neural correlates of distraction from visual food cues in patients with Anorexia nervosa and healthy controls.

Detailed Description
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Anorexia nervosa (AN) is an eating disorder with high morbidity and lifetime mortality. This eating disorder is mainly characterized by restricted food intake despite a severely low body weight. Given the pronounced self-starvation in AN, the investigation of homeostatic food processing, and its interaction with the reward system is of great scientific interest. Previous research in AN patients has almost exclusively focused on cortical, non-homeostatic (e.g., reward related) food processing. Therefore, the primary aim of the present study is to investigate metabolic gut-brain signaling by focusing on the responsivity of the hypothalamus (i.e., the core region of homeostatic control) and the mesocorticolimbic reward system. A secondary aim is to study the interaction between the mesocorticolimbic reward system and the homeostatic (i.e., hypothalamus) system. Metabolic gut-brain signaling will be assessed by applying a single-blind, randomized, crossover design of intragastric infusion of glucose or water. This approach allows the study of gut-brain signaling to the hypothalamus and the reward system by controlling for sensory aspects of food intake (sight, smell, and taste) in AN patients and healthy controls. Furthermore, we will measure how cognitive strategies to control the desire for visual food cues (top-down control) affect the mesocorticolimbic and hypothalamic systems in AN patients differently than in healthy controls. The interaction between the hypothalamus and the mesocorticolimbic reward system will be investigated using an effective connectivity analysis. Functional magnetic resonance imaging with high spatial resolution and with an optimized protocol for the investigation of the hypothalamus and the mesocorticolimbic reward system will be used to study for the first time homeostatic and non-homeostatic food cue processing in AN patients.

Official Title
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Homeostatic and Non-homeostatic Processing of Food Cues in Anorexia Nervosa

Conditions
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Healthy, Anorexia Nervosa

Intervention / Treatment
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* Other: Glucose
* Other: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: patients that meet the diagnostic criteria for AN (DSM-V criteria) Medically stable patients with a BMI < 17.5 kg/m² and > 13 kg/m²; Healthy controls with a BMI <25 kg/m² and >18.5 kg/m² Over Age of 18 years no other lifetimes or current medical illness that could potentially affect appetite or body weight right-handedness normal or corrected-to-normal vision Exclusion Criteria: history of head injury or surgery history of neurological disorder severe psychiatric comorbidity (psychosis, bipolar disorder, substance abuse) smoking borderline personality disorder current psychotropic medication inability to undergo fMRI scanning (e.g. metallic implants, claustrophobia, Pacemakers) pregnancy

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: intragastric glucose administration<br> | Other: Glucose<br>* 75 g of glucose dissolved in 300 ml of water<br>|
| Placebo Comparator: intragastric water administration<br> | Other: Placebo<br>* 300 ml of tap water<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Resting state brain activity using fMRI | Functional brain imaging will be employed to assess metabolic gut-brain signaling during a single blind, randomized cross-over design of gastric glucose vs. water infusion. | 35 min | 
| Experimental fMRI task | Participants will be asked to either view food or nonfood images or to down-regulate their emotional response by distracting themselves from the stimuli by solving an arithmetic task. | 20 min | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Analysis of hormonal satiety signaling | Blood is collected for the measurement of peripheral ghrelin. In total, three blood samples will be collected. | 30 min before scanning, 30 min after intragastric feeding, 60 min after intragastric feeding | 
| Self-report questionnaire regarding eating behavior (Dutch Eating Behavior Questionnaire) | Psychometric tests will be employed to assess eating behavior and eating disorder psychopathology (using the Dutch Eating Behavior Questionnaire). | 30 min |