The Impact of Protein Intake on Protein Metabolism During Intensified Training

Study Overview
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Brief Summary
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Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA). Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake. The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.

Official Title
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The Impact of Protein Intake on Amino Acid and Protein Metabolism, and Performance During Intensified Training

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Normal
* Dietary Supplement: Moderate
* Dietary Supplement: High
* Other: Controlled-diet


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy, male, endurance-trained participants who have run regularly more than 45 km or 4.5 hours/week Participants who are categorized at least very good based on a study by Shvartz & Reibold [40], in which peak rate of oxygen consumption(VO2 Peak) is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y), ≥47 ml/kg/min (40-44 y), ≥44 ml/kg/min (45-50 y) according to his age, Participants who can cover 10 km in less than 60 min after the VO2peak test and 5 km Time trial on session 2. Participants will be 18-50 years old. Participants are willing to abide by the compliance rules of this study Exclusion Criteria: Inability to meet health and physical activity guidelines according to the The Physical Activity Readiness Questionnaire for everyone. Female Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor Regular tobacco use Illicit drug use (e.g. growth hormone, testosterone, etc.)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Normal protein intake<br>participants will receive the controlled-diet containing 0.8 g protein /kg/day and the test drink containing 0.14 g protein/kg/d. | Dietary Supplement: Normal<br>* test drink which contains low amount of amino acids (0.14 g/kg/day)<br>Other: Controlled-diet<br>* the diet containing 0.8 g protein /kg/day<br>|
| Experimental: Moderate protein intake<br>participants will receive the controlled-diet containing 1.20 g protein /kg/day and the test drink containing 0.40 g protein/kg/d. | Dietary Supplement: Moderate<br>* test drink which contains moderate amount of amino acids (0.40 g/kg/day)<br>Other: Controlled-diet<br>* the diet containing 0.8 g protein /kg/day<br>|
| Experimental: High protein intake<br>participants will receive the controlled-diet containing 1.83 g protein /kg/day and the test drink containing 1.03 g protein/kg/d. | Dietary Supplement: High<br>* test drink which contains high amount of amino acids (1.03 g/kg/day)<br>Other: Controlled-diet<br>* the diet containing 0.8 g protein /kg/day<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in 24h whole body protein balance | Protein balance (mg/kg/d; protein synthesis - protein breakdown) will be measured using [15N]Glycine method on day 1 and day 4 to determine the change over the intensified training period to compare between protein levels | Difference between day 1 and day 4 whole body protein balance | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change from baseline physical performance (5 km time trial) 5days after the beginning of training | Before and after 4 days of intensified training period, Duration (seconds) for completing 5-km running on treadmill will be measured. | 5days after the beginning of training | 
| change from baseline Physical performance (muscle strength) 5days after the beginning of training | Maximal strength will be assessed by a maximal isometric voluntary contraction (MVC), which will be tested for the knee-extensors using a custom-made knee-extension apparatus after 4 days intensified training. | 5days after the beginning of training | 
| change from baseline Physical Performance (muscle power) 5days after the beginning of training | Peak lower body power will be measured by force platform after 4days-intensified training. | 5days after the beginning of training | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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exercise, performance, recovery, muscle, protein