Antibacterial Effectiveness of Two Root Canal Irrigants

Study Overview
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Brief Summary
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The aim of this randomized clinical trial is to compare the antibacterial effects of 1% sodium hypochlorite (NaOCl) and 2% chlorhexidine gluconate (CHX) during retreatment of teeth with apical periodontitis (AP).

Detailed Description
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Root canal-treated teeth with AP were randomly distributed in two groups according to the irrigant used. After removal of the previous filling material, bacteriological samples were taken from the canals before (S1) and after chemomechanical preparation using either NaOCl or CHX (S2), and after calcium hydroxide medication for 2-3 weeks (S3). 16S rRNA gene-based real-time qPCR was performed to detect and quantify total bacteria, Streptococcus species and Enterococcus faecalis.

Official Title
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Antibacterial Effectiveness of Two Root Canal Irrigants in Root-filled Teeth With Infection: A Randomized Clinical Trial

Conditions
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Periapical Periodontitis

Intervention / Treatment
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* Procedure: Retreatment of root-filled teeth with infection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Clinical diagnosis of apical periodontitis in a root-filled tooth assessed by radiography. Exclusion Criteria: Presence of gross carious lesions in the diagnosed tooth, Presence of fractures involving the periodontium of the diagnosed tooth, Presence of periodontal pockets more than 4 mm deep in the diagnosed tooth, Diagnosis of diabetes, Diagnosis of HIV infection, Other immunocompromising disease, Antibiotic therapy within the previous 3 months.

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 95 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: 1% sodium hypochlorite (NaOCl)<br>Retreatment of root-filled teeth with infection with 1% NaOCl as irrigation solution. Total bacterial counts, Streptococcal species and Enterococcus faecalis species were quantified using qPCR against 16SrRNA before irrigation (S1), after irrigation (S2), and after intracanal medication (S3). | Procedure: Retreatment of root-filled teeth with infection<br>* Root-filled teeth with infection are being treated with either 1% NaOCl or 2% CHX as disinfection irrigation solution.<br>|
| Active Comparator: 2% chlorhexidine gluconate (CHX)<br>Retreatment of root-filled teeth with infection with 2% CHX as irrigation solution. Total bacterial counts, Streptococcal species and Enterococcus faecalis species were quantified using qPCR against 16SrRNA before irrigation (S1), after irrigation (S2), and after intracanal medication (S3). | Procedure: Retreatment of root-filled teeth with infection<br>* Root-filled teeth with infection are being treated with either 1% NaOCl or 2% CHX as disinfection irrigation solution.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Periapical radiolucency (PAI- and Friedman-scores) |  | 12 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Bacterial load assessed by qPCR |  | 21 days | 
| Pyrosequencing of species found before and after the intervention |  | 21 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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endodontic retreatment, sodium hypochlorite, chlorhexidine, calcium hydroxide, Retreatment