Epithelial Mesenchymal Transition Markers in OSMF

Study Overview
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Brief Summary
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Oral Sub mucous Fibrosis (OSMF) is essentially an imbalance between collagen metabolism and wound healing mechanism induced by arecanut chewing habit. Clinically the disease progresses in stages with patients presenting with burning sensation, intolerance to spicy food, vesicles particularly on the palate, ulceration and dryness of the mouth , fibrosis of the oral mucosa, leading to lips, tongue, and palate rigidity and finally trismus. As the disease is progressively debilitating and has potential to turn in to malignant cancer a study was designed to assess if there any tissue or saliva markers that can be assessed for early diagnosis and indicate malignant transformation if any. Participants who had OSMF and habit history, patients without OSMF but habit history formed the case group where as normal patients without OSMF and no habit history were in control group. Eligible candidates who consented to participate in study were subjected to biopsy procedure and also their saliva samples were collected. Biopsy samples were subjected to immunohistochemistry (IHC) and polymerase chain reaction (PCR) to assess the EMT markers like vimentin, e-cadherin and collagen IV. miRNA copies were extracted from saliva and were subjected RT-PCR. Research question was: Is EMT a positive signature in OSMF. Does histopathological grading and dysplasia in OSMF have any correlation with EMT. Can aberrant EMT markers be a reliable indicator for risk assessment of early malignant transformation. Can expression of mi RNA 21 in saliva predict the disease severity and more importantly assess risk of early malignant transformation in OSMF.

Detailed Description
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Epithelial-mesenchymal transition (EMT) describes the process whereby cells shed their epithelial properties and adopt a more mesenchymal and invasive phenotype. A continuous molecular cross talk between epithelial and mesenchymal cells is required during embryonic development (epithelial mesenchymal transition) and probably plays an important role in pathologic process like wound healing and tumour progression. The development of carcinomas is due to the genetic changes within the target epithelium. As there is conversion of normal epithelium to potentially malignant and to squamous cell carcinoma, the stroma also changes from normal to activated or tumor associated called as the stromal reaction. 10 EMT can generate mesenchymal cells that express α-smooth muscle actin (αSMA), as seen in myofibroblasts. It is, however, unclear whether functional myofibroblasts seen in fibrosis or cancer derived from epithelial or endothelial cells Hence EMT markers in OSMF were assessed in this study. the following markers chosen as: miRNA-21 regulates cell proliferation, apoptosis and the epithelial to mesenchymal transition during neoplastic progression and is up regulated in a variety of cancers.An increased expression of miRNA-21 is seen in myofibroblasts.12 Since we know the role of myofibroblasts in OSMF, salivary expression of mi RNA- 21 was included as marker. Type IV collagen has the unique feature of self-associating through intramolecular covalent bonds, providing barrier and mechanical strength properties to basement membranes.The increased collagen deposition in OSMF involves numerous fibroblasts that are converted to myofibroblasts found in histologic sections of tissue specimens. an altered micro environment or stroma such as persistent myofibroblasts has been shown to result in tumorigenesis and tumor progression Cadherins are a class of type-1 trans-membrane proteins known for its calcium-dependent cell-cell adhesion property. E-cadherins are members of this protein family found in epithelial cells which serve important roles in cell adhesion by ensuring that cells within tissues are bound together. The loss of E-cadherin function or expression has been implicated in cancer progression and metastasis.E-cadherin expression in the head and neck epithelium is essential for the morphogenesis and homeostasis of epithelial tissues. The cadherin-mediated cell-cell contacts are required for the anchorage-dependent growth of epithelial cells. Hence lower expression of E- cadherin is a sign of loss of epithelial integrity The intermediate filament protein vimentin is involved in the regulation of cell behavior, morphology, and mechanical properties. Vimentin is a EMT marker expressed in connective tissue. Expression of vimentin in epithelium is a positive signature of EMT, and its evaluation in OSMF can unveil the importance of EMT signature in oral fibrosis.

Official Title
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Risk Assessment of Malignant Transformation in Oral Submucous Fibrosis Using Epithelial Mesenchymal Transition (EMT) Signatures in Tissue Samples and Saliva

Conditions
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Oral Submucous Fibrosis

Intervention / Treatment
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* Diagnostic Test: Biopsy tissue analysis
* Diagnostic Test: Saliva analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Any patient who had history of arecanut chewing habit with clinical manifestations of OSMF. Patients who had arecanut chewing habit history of more than 1 year and had no clinical symptoms of OSMF. Healthy human volunteers who are indicated for extraction of tooth and had no clinical features of OSMF and had no habit history Exclusion Criteria: Patients who had bleeding disorders patients who are diagnosed with salivary gland disorders that can alter salivary flow or composition

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patient with habits and having OSMF<br>Patients who had history of arecanut chewing habit in any form and composition and who were not undergone any treatment for their current condition i.e, OSMF | Diagnostic Test: Biopsy tissue analysis<br>* biopsy samples for IHC staining and PCR<br>Diagnostic Test: Saliva analysis<br>* Saliva samples for miRNA 21 expression analysis<br>|
| Patients with habits and had no clinical symptoms of OSMF<br>Patients who had history of arecanut chewing habit in any form and composition and had no symptoms of OSMF clinically | Diagnostic Test: Biopsy tissue analysis<br>* biopsy samples for IHC staining and PCR<br>Diagnostic Test: Saliva analysis<br>* Saliva samples for miRNA 21 expression analysis<br>|
| Healthy human volunteers<br>patients who reported no history of areacnut chewing habits and had no clinical symptoms of OSMF | Diagnostic Test: Biopsy tissue analysis<br>* biopsy samples for IHC staining and PCR<br>Diagnostic Test: Saliva analysis<br>* Saliva samples for miRNA 21 expression analysis<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in Expression of E-cadherin levels in all the groups in IHC | For IHC: The criteria used to define E-cadherin positive cells was brown staining in cell membrane of epithelial cells; the percentage of positively stained cells were scored as - Percentage of POSITIVE CELLS SCORE No cells 0 ≤ 10% 1 11% -50 % 2 51%-80% 3 ≥81% 4 Staining Intensity was also assessed in comparison with the positive control tissue and scored accordingly INTENSITY SCORE Negative 0 Weak 1 Moderate 2 Strong 3 | 10 days from sample collection | 
| Change in Expression of vimentin levels in all the groups in IHC | vimentin positive cells was brown staining in cytoplasm of epithelial cells; the percentage of positively stained cells were scored as - Percentage of POSITIVE CELLS SCORE No cells 0 ≤ 10% 1 11% -50 % 2 51%-80% 3 ≥81% 4 Staining Intensity was also assessed in comparison with the positive control tissue and scored accordingly INTENSITY SCORE Negative 0 Weak 1 Moderate 2 Strong 3 | 10 days from sample collection | 
| Change in Expression of collagen IV levels in all the groups in IHC | collagen IV positive cells was brown staining in basement membrane cells; the percentage of positively stained cells were scored as - Percentage of POSITIVE CELLS SCORE No cells 0 ≤ 10% 1 11% -50 % 2 51%-80% 3 ≥81% 4 Staining Intensity was also assessed in comparison with the positive control tissue and scored accordingly INTENSITY SCORE Negative 0 Weak 1 Moderate 2 Strong 3 | 10 days from sample collection | 
| Change in Expression of miRNA 21 in all three groups using Real Time-PCR | Considering the expression of miRNA21 in normal sample (N) as 1, the expression of all the samples were calculated The formula used is: number of copies = (amount * 6.022x1023) / (length * 1x109 * 650) | 10 days from sample collection | 
| CHange in E cadherin levels in PCR | After extraction of DNA from biopsy samples the primers for E-cadherin were designed using Primer Express software, synthesized and HPLC purified for real time PCR for quantification. | 20 days after sample collection | 
| Change in vimentin levels in PCR | After extraction of DNA from the biopsy samples the primers for Vimentin were designed using Primer Express software, synthesized and HPLC purified for real time PCR for quantification | 20 days after sample collection | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in e-cadherin expression with respect to disease category clinically in OSMF patients with habits | E cadherin level in tissue samples with respect to different Clinical expression of disease according to Kerr et al classification system. | 15 days from sample collection | 
| Change in e-cadherin expression with respect to disease category histologically in OSMF patients with habits | E cadherin level in tissue samples with respect to different Clinical expression of disease according to Kanna and Andrade et al classification system. | 15 days from sample collection | 
| Change in vimentin expression with respect to disease category clinically in OSMF patients with habits | Vimentin level in tissue samples with respect to different Clinical expression according to Kerr et al classification system. | 15 days from sample collection | 
| Change in vimentin expression with respect to disease category histologically in OSMF patients with habits | Vimentin level in tissue samples with respect to different histological expression according to Khanna and Andrade classification system. | 15 days from sample collection | 
| Change in collagen IV expression with respect to disease category clinically in OSMF patients with habits | Collagen-IV level in tissue samples with respect to different Clinical expression category according to Kerr et al classification system. | 15 days from sample collection | 
| Change in collagen IV expression with respect to disease category hstologically in OSMF patients with habits | Collagen-IV level in tissue samples with respect to different histological expression according to Khanna and Andred et al classification system. | 15 days from sample collection | 
| Change in mi RNA 21 expression with respect to disease category clinically in OSMF patients with habits | miRNA 21 level in saliva samples with respect to different Clinical expression of OSMF according to Kerr et al classification system. | 15 days from sample collection | 
| Change in mi RNA 21 expression with respect to disease category histologically in OSMF patients with habits | miRNA 21 level in saliva samples with respect to different histological expression according to Khanna and Andred et al classification system. | 15 days from sample collection | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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OSMF, EMT, miRNA 21, vimentin, e-cadherin