Cytokine Assay in Tears of Healthy Children and With Vernal Keratoconjunctivitis: Case Control Study and Monitoring of Cases at 6 Months

Study Overview
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Brief Summary
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The ocular surface may be the seat of an inflammatory process in many pathology as vernal keratoconjunctivitis. It has been shown that inflammatory cytokines produced by the cells themselves and the constituent cells of the target organ, are the main factors that cause inflammation. The main objective of our study was to compare the concentration of 40 cytokines in vernal keratoconjunctivitis and in control subjects.

Detailed Description
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The ocular surface may be the seat of an inflammatory process in many pathology as vernal keratoconjunctivitis. It has been shown that inflammatory cytokines produced by the cells themselves and the constituent cells of the target organ, are the main factors that cause inflammation. The main objective of our study was to compare the concentration of 40 cytokines in vernal keratoconjunctivitis and in control subjects.

Official Title
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Cytokine Assay in Tears of Healthy Children and With Vernal Keratocunjonctivitis: Case Control Study and Monitoring of Cases at 6 Months

Conditions
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Vernal Keratoconjunctivitis, Healthy Children

Intervention / Treatment
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* Procedure: Cytokins assay in tears


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Childrens between 3 and 18 years Affiliated at system of French social security Signed by the holders of parental authority of the sheet and written consent to participate in this study First consultation at Clermont-Ferrand Hospital for vernal keratocunjunctivitis Exclusion Criteria: Subject suffering from acute or chronic eye disease in which the physician investigator resound with the results: uveitis, glaucoma, retinopathy Subject who had undergone eye surgery Subject having an infection the day of collection: ENT, digestive, urinary Subject with cancer, mental illness or any other condition in which the physician investigator sound informed consent and / or the results Pregnant subject Subject with contact lenses Subject who judged by the investigating physician not enough cooperating to allow a safe collection

Ages Eligible for Study
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Minimum Age: 3 Years
Maximum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| vernal keratoconjunctivitis<br>The main objective of our study was to compare the concentration of 40 cytokines in vernal keratoconjonctivitis and in control subjects | Procedure: Cytokins assay in tears<br> <br> |
| healthy children<br>The main objective of our study was to compare the concentration of 40 cytokines in vernal keratoconjunctivitis and in control subjects | Procedure: Cytokins assay in tears<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Concentration of 40 cytokines in tears | Comparaison of concentration of 40 cytokines in tears between healthy children and children with vernal keratoconjunctivitis | at time 0 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Validation of normal range of cytokines in tears of healthy children |  | at 6 month | 
| Modification of the inflammatory profile according to the clinical severity and treatment |  | at 6 month | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Vernal keratoconjunctivitis, Cytokines, Mediators, Tears