Biomarker Differences in Samples From Patients With Undifferentiated Sarcomas

Study Overview
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Brief Summary
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This clinical trial studies biomarker differences in samples from patients with undifferentiated sarcomas. Studying biomarker in tissue samples from patients with cancer in the laboratory may help doctors identify and learn more about biomarkers related to cancer

Detailed Description
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Study Subtype: Ancillary/Correlative Observational Study Model: Cohort Time Perspective: Prospective Biospecimen Retention: Samples With DNA Biospecimen Description: Tissue Study Population Description: Patients from ARST0332 study Sampling Method: Non-Probability Sample PRIMARY OBJECTIVES: I. To determine what proportion of unclassifiable sarcomas are unclassifiable because the initial evaluation was not sufficiently comprehensive versus what proportion can be regarded as true undifferentiated sarcoma when a comprehensive assessment has been performed. II. To develop formal diagnostic criteria for establishing the diagnosis of undifferentiated sarcoma. III. To determine whether undifferentiated sarcoma can be subdivided into separate and distinct pathologic entities that are distinguishable by light microscopy, immunohistochemistry, single nucleotide polymorphism (SNP) array profiling, or clinical features. IV. To determine whether undifferentiated sarcomas with specific actionable mutations can be identified based on their histologic appearance, immunohistochemical staining characteristics, or SNP array profiling features. OUTLINE: Tissue samples are analyzed for loss of heterozygosity (LOH) and SNP array profiling using microarray and immunohistochemistry.

Official Title
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Observational - SNP Array Analysis of Undifferentiated Sarcomas

Conditions
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Soft Tissue Sarcoma

Intervention / Treatment
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* Other: laboratory biomarker analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 49 cases of undifferentiated sarcomas from ARST0332 (additional cases may be ascertained as eligible for this current project that also fall under ARST0332 to be requested later if necessary)
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Basic science (SNP array analysis)<br>Tissue samples are analyzed using laboratory biomarker analysis for LOH and SNP array profiling using microarray and immunohistochemistry. | Other: laboratory biomarker analysis<br>* Correlative studies<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Proportion of unclassifiable sarcomas that are unclassifiable |  | Baseline | 
| Development of formal diagnostic criteria for establishing the diagnosis of undifferentiated sarcoma |  | Baseline | 
| Separate and distinct pathologic entities of undifferentiated sarcoma that are distinguishable by light microscopy, immunohistochemistry, SNP array profiling, or clinical features |  | Baseline | 
| Specific actionable mutations that can be identified based on their histologic appearance, immunohistochemical staining characteristics, or SNP array profiling features |  | Baseline |