The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level

Study Overview
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Brief Summary
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The postprandial increase in MDA level following meat meal could cause modification to LDL particles in healthy volunteers. Co-consumption of food drived polyphenols with the meat will result in a significant decrease in LDL modification.

Official Title
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Nutritional Study:The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level

Conditions
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Healthy

Intervention / Treatment
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* Other: frozen meat cutlets meal
* Other: meat meals


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy adults, normal BMI Exclusion Criteria: Metabolic disorders Smokers Drinkers Taking nutritional supplement

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: frozen meat<br>250 g of frozen meat meal for 4 days | Other: frozen meat cutlets meal<br>* 250 g of meat cutlets<br>Other: meat meals<br>* 250 g of meat meal for 4 days<br>|
| Other: frozen meat and wine<br>250 g of frozen meat and red wine for 4 days | Other: frozen meat cutlets meal<br>* 250 g of meat cutlets<br>Other: meat meals<br>* 250 g of meat meal for 4 days<br>|
| Other: fresh meat<br>250 g of fresh meat meal | Other: frozen meat cutlets meal<br>* 250 g of meat cutlets<br>Other: meat meals<br>* 250 g of meat meal for 4 days<br>|
| Other: fresh meat and wine<br>250 g of fresh meat meal | Other: frozen meat cutlets meal<br>* 250 g of meat cutlets<br>Other: meat meals<br>* 250 g of meat meal for 4 days<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change in plasma MDA levels following meat meal | After an overnight fast baseline (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The subjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 and 6 h after the meal was eaten. Serum was separated from whole blood by centrifugation (910 g, 15 min), and MDA was evaluated by HPLC in plasma samples | Fasting before meal and 3, and 6 h postprandial, and fasting levels | 
| Changes in MDA concentration on LDL fraction following meat meals | Subject consumed the test meals for 4 days. Day 1, (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The sbjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 h after the meal was eaten. Blood was collected at day 4, 3 h after the meal was eaten, at the morning of day 5.Plasma LDL was isolated using a single discontinuous density gradient ultracentrifugation. The protein concentration was determined based on the method of Bradford. MDA concentration was evaluated by HPLC. | Fasting, before meal was eaten, 3 h following meal, at day 4, 3 h following meal consumption, and day 5 following over nigth fast | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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LDL, MDA, meat