Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

Study Overview
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Brief Summary
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This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect. Therefore, this study hypothesizes that: H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise. H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism. H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels. H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism. H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.

Official Title
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The Effect of an Acute Maximal Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

Conditions
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Uncoupling Protein-1, Polymorphism, Restriction Fragment Length, Irisin

Intervention / Treatment
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* Other: Yo-Yo intermittent recovery test (level 1)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Athletes group: Must be basketball, or volleyball, or handball players. Must be subjects who have been training regularly for at least 3 months. Sedentary group: * Must be subjects who have not been training regularly for at least 3 months. For both groups: Healthy. Non obese (body mass index < 30). Aged between 18 and 35 years. Exclusion Criteria: For both groups: Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Being obese (body mass index > 30). Having an acute or chronic injury which is limiting physical performance. Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples. Being injured or sick during the study.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Athletes Group<br>The athletes group will be composed of healthy, non obese (body mass index < 30), male basketball, volleyball and handball players aged between 18 and 35 years who have been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered. | Other: Yo-Yo intermittent recovery test (level 1)<br>* The Yo-Yo intermittent recovery test (level 1) will be administered once.<br>|
| Experimental: Sedentary Group<br>The sedentary group will be composed of healthy, non obese (body mass index < 30), male aged between 18 and 35 years who have not been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered. | Other: Yo-Yo intermittent recovery test (level 1)<br>* The Yo-Yo intermittent recovery test (level 1) will be administered once.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | 
| Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | 
| Uncoupling protein-1-3826 A/G polymorphism determination | Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory. | 15 minutes before the Yo-Yo intermittent recovery test (level 1) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Interleukine-6 analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | 
| Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Nitric oxide analyzes were performed in the laboratory. | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Irisin, Beige fat cell, Fat tissue, Athletes, Sedentary people