Salivary Bacterial Profiles in Relation to Periodontal Treatment

Study Overview
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Brief Summary
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The purpose of the present investigation is to record and compare bacterial compositions in subgingival plaque samples and saliva samples in subjects receiving periodontal treatment. The hypothesis is that the composition of the salivary microbiota might reflect local bacterial alterations in relation to periodontal treatment.

Detailed Description
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In this study we plan to enroll 35 subjects with manifest periodontitis, which will be treated according to national and international guidelines. Clinical registration and collection of subgingival plaque samples and saliva samples will be performed at baseline and 2, 6 and 12 weeks after treatment.

Official Title
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Short and Long Term Alterations of Subgingival and Salivary Microbiota in Relation to Periodontal Treatment

Conditions
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Periodontitis

Intervention / Treatment
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* Procedure: Conventional periodontal treatment


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Manifest chronic periodontitis according to international classification Exclusion Criteria: Other treatment requiring oral conditions Systemic antibiotics within the latest 6 months Systemic diseases and medications with known associations to periodontitis Pregnancy and breastfeeding

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 99 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Conventional periodontal treatment<br>35 subjects diagnosed with manifest periodontitis, which will all receive standardized treatment according to protocol. In brief, this treatment includes professional tooth cleaning and thorough instruction in self-performed oral hygiene procedures. Clinical registrations and collection of microbiological samples (subgingival and saliva) will performed at baseline and 2, 6 and 12 weeks after treatment. | Procedure: Conventional periodontal treatment<br>* Non-surgical periodontal treatment including thorough instruction in self-performed oral hygiene<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in α-diversity in saliva samples collected . | Comparison of α-diversity in saliva samples collected before periodontal treatment and 12 weeks after treatment | Baseline and 12 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Specis specific changes in saliva samples collected | Comparison of relative abundance of bacterial species in saliva samples collected before periodontal treatment to samples collected 12 weeks after treatment | Baseline and 12 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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periodontitis, saliva, bacteria