Impact of Cold Ischemia on Pulmonary Endothelial Dysfunction in Ex-vivo Pulmonary Reconditioning

Study Overview
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Brief Summary
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The study population will be patients with planned double-lung transplant, with preoperative ex-vivo reconditioning procedure. The ex-vivo procedure will be performed for lungs considered as limit to be directly implanted according to the national classification or in case of Maastrich 3 decision. They required a rehabilitation procedure like ex-vivo to determine their quality. Once the procedure initiated, the unused remaining liquid will be retrieved for the first gasometry usually performed. This will be the time T1 of sampling. The unused remaining liquid will be retrieved for the gasometry of end of procedure usually performed, before the graft cooling. This will be the time T2. The purge liquid of first lung at the declamping of the pulmonary artery will be retrieved at the time of unclamping (time T3 of sampling). The purge liquid of the second lung at the declamping of the pulmonary artery will be retrieved with the same procedure (time T4 of sampling). The samples will be analysed with ELISA technique. The endothelial dysfunction will be evaluated with plasma concentration of heparan sulphate, syndecan-1, endothelin-1 and i-NOS.

Official Title
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Impact of Cold Ischemia on Pulmonary Endothelial Dysfunction in Ex-vivo Pulmonary Reconditioning

Conditions
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Lung Transplant

Intervention / Treatment
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* Other: Plasma concentration analysis during and after ex-vivo pulmonary reconditioning


Participation Criteria
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Eligibility Criteria
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Selection Criteria: patient aged > 18 years old patient with healthcare insurance non-opposition of the patient patient with usual criteria for lung transplant and planned lung transplant after meeting of staff transplant Non Selection Criteria: participation refusal patient under tutorship or guardianship protected adult Inclusion Criteria: selected patient with Biomedecine Agency agreement for graft allocation off tour Exclusion Criteria: selected patient with Biomedicine Agency agreement for standard allocation graft

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: Plasma concentration analysis during and after ex-vivo pulmonary reconditioning|The pulmonary endothelial dysfunction will be evaluated with plasma concentration of heparan sulphate, syndecan-1, endothelin-1 and i-NOS.|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Correlation between time of cold ischemia and endothelial dysfunction | Plasma concentration in heparin sulphate, syndecan-1, endothelin-1 and i-NOS | 24 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Correlation between time of cold ischemia and systemic inflammation | Plasma concentration in IL-1β, IL-8, IL-10 and TNF-α | 24 hours |