Expression of Toll-Like Receptors 2 and 4 in Asthmatic Patients.

Study Overview
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Brief Summary
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Introduction: Recently it has involved the role of TLR (toll-like receptors) in the pathogenesis of asthma. TLRs are a family of proteins responsible for the recognition of Pathogen-Associated Molecular Patters (PAMPs). The possible role that TLRs play in IgE-dependent asthma (not eosinophilic) and its recognition as a new endotype asthma, could open new therapeutic expectations. Objective: To analyze the expression of TLR 2 and 4 in monocytes/ macrophages and neutrophils in peripheral blood and induced sputum from asthmatic patients

Detailed Description
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The investigators studied patients with asthma. All patients underwent the same day an induced sputum, pulmonary function studies, fractional exhaled nitric oxide, blood total IgE and skin test by prick technique. Asthmatic patients were classified according to the criteria of the GINA 2012. The cellular composition was analyzed by flow cytometry. Expression of TLR2 and TLR4 were analyzed using monoclonal antibodies conjugated with fluorochromes appropriate.

Official Title
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Expression of Toll-Like Receptors 2 and 4 in Asthmatic Patients.

Conditions
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Asthma

Intervention / Treatment
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* Other: TLRs


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adults with persistent asthma (defined as per the Global INitiative for Asthma Management (GINA) criteria) Exclusion Criteria: If they had a respiratory tract infection or asthma exacerbation within 30 days prior to inclusion If they were receiving oral steroids or immunosuppressive treatments.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: toll like receptor (TLRs)<br>TLRs are a family of proteins responsible for the recognition of Pathogen-Associated Molecular Patters | Other: TLRs<br>* Recently it has involved the role of TLR (toll-like receptors) in the pathogenesis of asthma.The possible role that TLRs play in the asthma and its recognition as a new endotype asthma, could open new therapeutic expectations.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic | The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic (IgEnodep).This could suggest that in this type of asthma the defense mechanism of neutrophils could be altered by decreasing expression of these receptors on their cell surface. This mechanism would still be defined. Probably the respiratory infections play an important role. | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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asthma, toll like receptor, innate immunity