Acute Effects of Endurance Exercise on Breastmilk Composition

Study Overview
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Brief Summary
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The investigators will determine the acute effect of exercise on breastmilk composition. Participants will come in to the lab on three different days, out of which they will exercise on two of these days and rest in the lab on the third day. The conditions will be randomly allocated to each participant. Breastmilk samples will be obtained in the morning of each test day, as well as immediately after, one hour after and four hours after the exercise/rest condition. Dietary intake will be standardized on the test days. At the time of study commencement, the trial (including planned analyses) is not fully funded. Additional milk will be stored for future analyses (not yet specified) when funding is secured.

Official Title
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Acute Effects of Endurance Exercise on Breastmilk Composition: Pilot Study

Conditions
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Breastmilk

Intervention / Treatment
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* Behavioral: High intensity interval training
* Behavioral: Moderate intensity training
* Behavioral: Resting


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 6 to 12 weeks postpartum Exclusive breastfeeding Term birth Exclusion Criteria: Known cardiovascular disease Type 1 or 2 diabetes mellitus Limited ability to exercise

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Sequential Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Breastmilk<br>Randomized intervention order. | Behavioral: High intensity interval training<br>* Four times four minutes treadmill interval training<br>Behavioral: Moderate intensity training<br>* Moderate intensity treadmill training<br>Behavioral: Resting<br>* No training<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Breastmilk metabolite composition | Metabolomics profiling | Change from before exercise to after exercise (+0 hour, +1 hour, and + 4 hours) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Adiponectin | Concentration in breastmilk | Change from before exercise to after exercise (+0 hour, +1 hour, and + 4 hours) | 
| Leptin | Concentration in breastmilk | Change from before exercise to after exercise (+0 hour, +1 hour, and + 4 hours) | 
| Insulin | Concentration in breastmilk | Change from before exercise to after exercise (+0 hour, +1 hour, and + 4 hours) | 
| Insulin Growth Factor-1 | Concentration in breastmilk | Change from before exercise to after exercise (+0 hour, +1 hour, and + 4 hours) | 
| Cytokine profiling (Multiplex 27) | Concentration in breastmilk | Change from before exercise to after exercise (+0 hour, +1 hour, and + 4 hours) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Exercise