Serum Exosomal Long Noncoding RNAs as Potential Biomarkers for Lung Cancer Diagnosis

Study Overview
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Brief Summary
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The study is to investigate the sensitivity and specificity of serum exosome noncoding RNA as a biomarker for the diagnosis of lung cancer

Detailed Description
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Conventional tumor markers for non-invasive diagnosis of Lung cancer (LC) exhibit insufficient sensitivity and specificity to facilitate detection of early lung cancer (ELC). The investigators aimed to identify ELC-specific exosomal lncRNA biomarkers that are highly sensitive and stable for the non-invasive diagnosis of ELC.Hence, in the present study, exosomes from the plasma of five healthy individuals and 30 LC patients and from culture media of four human bronchial epithelial cells and four cancer cells were isolated. Exosomal RNA profiling was performed using RNA sequencing to identify LC specific exosomal lncRNAs.

Official Title
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Serum Exosomal Long Noncoding RNAs as Potential Biomarkers for Lung Cancer Diagnosis

Conditions
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Lung Cancer (Diagnosis)

Intervention / Treatment
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* Diagnostic Test: collect samples


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subjects are willing to sign the informed consent form; Normal subjects have no evidence of any disease; Lung cancer patients are 18 to 75 years old, diagnosed as lung cancer by histopathology or cytopathology Exclusion Criteria: People are unwilling to sign the informed consent form; Patients with heart disease, rheumatic disease, allergic disease, COPD, pulmonary fibrosis, diabetes, thyroid disease, liver, kidney, brain disease and hematopoietic system disease; Pregnant or lactating women; Patients did not cooperate or participate in other clinical trials

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| lung cancer patients<br> | Diagnostic Test: collect samples<br>* collect serum samples and clinical features<br>|
| normol volunteers<br> | Diagnostic Test: collect samples<br>* collect serum samples and clinical features<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The expression levels of serum exosome long non-coding RNA |  | the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study | 
| the expression levels of tumor biomarkers such as CEA, NSE, SCC, CYFR2A-1 |  | the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study | 
| the CT scans of the lung for the patients |  | the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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exosomes；lncRNA； lung cancer；