The Effects of Antibiotic Combinations on Stem Cells

Study Overview
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Brief Summary
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The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Detailed Description
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The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Official Title
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The Effects of Antibiotic Combinations on Mesenchymal Stem Cells Derived From Apical Papilla

Conditions
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Stem Cells, Anti-Bacterial Agents

Intervention / Treatment
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* Drug: Differentiation Markers


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients with open apex third molar teeth for extraction indication Exclusion Criteria: Patients with mature apex third molar teeth for extraction indication

Ages Eligible for Study
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Minimum Age: 16 Years
Maximum Age: 22 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Triple antibiotic paste(TAP)<br>ciprofloxacin, metronidazole and minocycline | Drug: Differentiation Markers<br>* Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.<br>* Other names: Gene expression levels;|
| Doble antibiotic paste(DAP)<br>ciprofloxacin and metronidazole | Drug: Differentiation Markers<br>* Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.<br>* Other names: Gene expression levels;|
| Modified triple antibiotic paste(mTAP)<br>ciprofloxacin, metronidazole and cefaclor | Drug: Differentiation Markers<br>* Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.<br>* Other names: Gene expression levels;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurment of Cell Viability and Apoptosis | To evaluate cell viability and apoptosis MuseCell Analyzer Count and Viability kit (Merck Millipore, Taufkirchen, Germany) was used according to the manufacturer's protocol. | 1 year | 
| Measurment of cell differantiation | To determine gene expression levels, total RNA of SCAP was extracted using the Ambion PureLink RNA mini kit (Invitrogen, US) and the Roche cell RNA Kit (Penzberg, Germany) according to the manufacturer's instructions. | 1 year |