Inflammation, Platelets and Sickle Cell Disease

Study Overview
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Brief Summary
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Sickle cell disease (SCD) is an autosomal recessive genetic disorder linked to a single mutation on beta-globin chains. This leads to red blood cell deformation and chronic hemolysis which can result in vaso-occlusive events, anemia and vasculopathy. Pathophysiology is incompletely understood, and beyond red blood cell's abnormalities this involves hemostasis and innate immunity. The aim of our study is to describe the mechanisms of thrombo-inflammation during the vaso-occlusive crisis (VOC) in adults with sickle cell disease.

Detailed Description
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Pathophysiology of sickle cell disease is incompletely understood. The typical change of red blood cells into sickle cells lead to post-capillary stream abnormalities. This phenomenon is responsible of ischemia-reperfusion injuries. Chronic hemolysis is the second part of the pathophysiology. The consequences are vasoconstriction, endothelial lesions, chronic inflammation, hemostasis and platelets activation. Thrombo-inflammation concept was proposed by Tanguay to describe the interactions between hemostasis, platelets and innate immune cells (neutrophils polynuclear) during thrombotic process. Since this time, this concept was largely described in many clinical situations such as septicemia, COVID-19, coronaropathy, auto-immune diseases and sickle cell disease. In this project, we will study platelets activation and thrombo-inflammation markers in the beginning of a vaso-occlusive crisis, during the crisis and two months after the crisis. Blood samples will be collected during a routine care sample. Biological markers studied will be: Soluble and surface markers of platelet activation; platelet-leukocytes aggregates Plasmatic eicosanoids produced by platelets (TXB2) and immune cells In vitro platelets reactivity; dynamic thrombus formation in normal and pathologic arterial blood stream Platelet's inflammasome

Official Title
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Inflammation, Platelets and Sickle Cell Disease

Conditions
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Platelet Activation, Thromboinflammation, Sickle Cell Disease

Intervention / Treatment
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* Biological: Blood sampling


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient with sickle cell disease diagnosis, hospitalized in emergency department and/or internal medicine department Patient older than 18 years Written consent to participate to the study Patient with health insurance Patient able to receive information about the study Exclusion Criteria: Age < 18 years Non consent to participate to the study Women in pregnancy or breastfeeding Treatment with aspirin or non steroidal anti inflammatory drug Protected patient Patient already involved in a study requiring collection of additional biological samples

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 99 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Biological: Blood sampling|the blood sample will be taken during a planned blood sampling|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To evaluate mechanisms of platelet activation in the thrombo-inflammation process during a vaso-occlusive crisis. | by flow cytometry analysis | First 48h of hospitalization | 
| To evaluate mechanisms of platelet activation in the thrombo-inflammation process during a vaso-occlusive crisis. | by flow cytometry analysis | Day 13 (+/- 2 days) | 
| To evaluate mechanisms of platelet activation in the thrombo-inflammation process during a vaso-occlusive crisis. | by flow cytometry analysis | At day 60 (+/- 2 days) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Sickle cell disease, vaso-occlusive crisis, platelets, thrombo-inflammation