Embryo Aneuploidies and Morphokinetics in Ovarian Stimulated and Non Stimulated IVF Cycles

Study Overview
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Brief Summary
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The purpose of this study is to compare the number of euploid embryos obtained per metaphase II oocyte in unstimulated (modified natural cycles) and stimulated IVF cycles.

Detailed Description
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Effects of ovarian stimulation on human embryo quality is still a subject of study. When natural and stimulated cycles have been compared, no differences have been observed in terms of embryo cleavage capacity, oocyte and embryo aneuploidy rate or incidence of aneuploidy in aborted foetuses. When mild and conventional doses of gonadotropins have been compared, a higher proportion of good morphological quality embryos are observed in the former. On the contrary, we showed that ovarian stimulation does not increase significantly the embryo aneuploidy rate in IVF derived human embryos when unstimulated and stimulated cycles were compared in oocyte donors. In this study, we want to compare the embryo aneuploidy rate between unstimulated and stimulated cycles in infertile patients, subjected to both IVF cycles with blastocyst biopsy and NGS-PGS. A comparison of the morphokinetics will also be performed.

Official Title
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Embryo Aneuploidies and Morphokinetics in Ovarian Stimulated and Non Stimulated Cycles. An Intrapatient Comparison Using Next Generation Sequencing in Biopsy of Blastocysts and Time Lapse Evaluation.

Conditions
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Euploid Embryos

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Women 30-38 years old (inclusive) with regular menstrual cycles (25-35 days) AntiMülleriana Hormone >5 pmol/L. Undergoing IVF treatment Exclusion Criteria: BMI >30 kg/m2 repeated miscarriages implantation failure uterine or adnexal pathology Unable to be treated with gonadotrophins for the ovarian hyperstimulation history of low response to ovarian hyperstimulation participation in another study with PGS for monogenic pathology Cryptozoospermia in male partner

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 38 Years

Sexes Eligible for Study
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Female

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Natural Cycle<br>The patient will not undergo hyperstimulation rather a natural cycle | |
| Stimulated Cycle<br>The 80 patients will undergo hyperstimulation cycle as per the clinical practice planned for the patient. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Comparison of the number of euploid embryos from Metaphase II oocytes in different cohorts of embryos. | Number of metaphase II oocyte per euploid embryo in unstimulated and stimulated cycles. | 30 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Incidence of embryo aneuploidies in two cohorts of embryos. | Incidence of embryo aneuploidies between the unstimulated and stimulated cycle in the same patient | 30 days | 
| Morphokinetic evaluation of embryos in two different cohorts of embryos. | Morphokinetic evaluation of the embryos obtained in an unstimulated and stimulated cycle | 30 days |