Bortezomib, Total Marrow Irradiation, Fludarabine Phosphate, and Melphalan in Treating Patients Undergoing Donor Peripheral Blood Stem Cell Transplant For High-Risk Stage I or II Multiple Myeloma

Study Overview
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Brief Summary
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RATIONALE: Bortezomib may stop the growth of cancer cells by blocking some of the enzymes needed for cell growth. Giving chemotherapy drugs, such as fludarabine phosphate and melphalan, and total marrow irradiation before a donor peripheral blood stem cell transplant helps stop the growth of cancer cells and helps stop the patient's immune system from rejecting the donor's stem cells. When the healthy stem cells from a donor are infused into the patient they may help the patient's bone marrow make stem cells, red blood cells, white blood cells, and platelets. PURPOSE: This phase I trial is studying the side effects and best dose of bortezomib when given together with fludarabine phosphate and melphalan with or without total marrow irradiation in treating patients undergoing donor peripheral blood stem cell transplant for high-risk stage I or II multiple myeloma.

Detailed Description
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PRIMARY OBJECTIVES: I. To determine the feasibility of escalating doses of bortezomib with or without total marrow irradiation (TMI) at a fixed dose of 900 cGy in combination with fludarabine (FLU) and melphalan (MEL) as preparative regimen for allogeneic hematopoietic stem cell transplant in patients with high risk multiple myeloma who have human leukocyte antigen (HLA) matched donor (sibling or matched unrelated donor). II. To describe toxicities, maximum tolerated dose (MTD) and dose limiting toxicities (DLT) of this preparative regimen. SECONDARY OBJECTIVES: I. To evaluate the frequency of clinical response, i.e., complete response [CR], partial response [PR], very good partial response [VGPR]) at 6 month and 1 year post transplant. II. To evaluate the frequency of primary and secondary engraftment failure. III. To evaluate the time to neutrophil and platelet engraftment. IV. To evaluate the incidence of acute and chronic graft-versus-host disease (GVHD). V. To evaluate progression-free survival. VI. To evaluate overall survival. VII. To evaluate minimal residual disease (MRD) at 6 months and 1 year post transplant by flow cytometry in the bone marrow. OUTLINE: This is a dose-escalation study of bortezomib. Patients are assigned to 1 of 2 treatment groups. GROUP I (patients eligible for TMI): Patients receive fludarabine phosphate intravenously (IV) on days -9 to -5 and melphalan IV on day -4. Patients also undergo TMI twice daily (BID) on days -9 to -7. If no DLT is observed in the first cohort, bortezomib IV will be added on days -6 and -3 for subsequent cohorts. GROUP II (patients ineligible for TMI): Patients receive fludarabine phosphate IV and melphalan IV as in Group I. Patients also receive bortezomib IV on days -6, -3, 1, and 4. TRANSPLANT: All patients undergo allogeneic peripheral blood stem cell (PBSC) transplant on day 0. GVHD PROPHYLAXIS: All patients receive tacrolimus IV or orally (PO) and sirolimus PO beginning on day -3. After completion of study treatment, patients are followed up at day 100, 6 months, and then annually thereafter for up to 4 years.

Official Title
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Phase I Study of Bortezomib With or Without Total Marrow Irradiation (TMI) Using Intensity Modulated Radiation Therapy (IMRT) in Combination With Fludarabine (FLU) and Melphalan (MEL) as a Preparative Regimen for Allogeneic Hematopoietic Stem Cell (HSC) Transplantation in Patients With High Risk Multiple Myeloma

Conditions
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Autologous Hematopoietic Stem Cell Transplant Recipient, Loss of Chromosome 17p, Plasma Cell Leukemia, Recurrent Plasma Cell Myeloma, Refractory Plasma Cell Myeloma

Intervention / Treatment
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* Drug: bortezomib
* Drug: fludarabine phosphate
* Other: melphalan
* Radiation: total marrow irradiation
* Drug: tacrolimus
* Drug: sirolimus
* Procedure: peripheral blood stem cell transplantation
* Other: laboratory biomarker analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Recipient must have signed a voluntary, informed consent in accordance with institutional and federal guidelines Recipients must have histopathologically confirmed diagnosis of multiple myeloma Age: Stratum I (TMI containing arm): 18-60 years of age Stratum II (non TMI arm): 18-70 years of age Patients with primary progressive disease on induction therapy with new targeted therapies Relapsed/refractory disease on new targeted therapies, i.e. thalidomide, lenalidomide, bortezomib, or other new novel agents such as carfilzomib, pomalidomide Patients with relapsed multiple myeloma following previous autologous stem cell transplant Plasma cell leukemia at diagnosis High-risk patients with presence of chromosome 17p deletion (> 60%) in the bone marrow by fluorescence in situ hybridization (FISH); patients are not required to have prior autologous stem cell transplant Able to lie supine for approximately 60 minutes, the anticipated duration of each treatment session Performance status evaluated by Eastern Cooperative Oncology Group (ECOG) or Karnofsky Performance Scales (KPS) patients must have a score of 0-II (ECOG) or >= 70% (KPS) Cardiac ejection fraction >= 50% by multiple gate acquisition (MUGA) scan and/or by echocardiogram Forced expiratory volume in one second (FEV1) >= 50% Diffusing lung capacity for carbon monoxide (DLCO) >= 50% Creatinine clearance or glomerular filtration rate (GFR) >= 60 ml/min Serum bilirubin =< 2.0 mg/dl Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) =< 2.5 times the institutional upper limits of normal Pre-treatment tests must be performed within 30 days prior to enrollment No other medical and or psychosocial problems, which in the opinion of the primary physician or principal investigator would place the patient at unacceptable risk from this regimen Patients with no previous radiation or up to a maximum 2000 cGy to non thoracic-spine and rib bone lesions or < 20% of bone marrow are eligible for TMI conditioning regimen Patients with previous history of irradiation at any dose to thoracic-spine, ribs or >= 20% of bone marrow cannot undergo TMI and will be eligible for bortezomib, fludarabine, and melphalan regimen Stratum II); patients can be enrolled on stratum II at their physician's discretion or if patients decline radiation therapy DONOR: Any matched sibling donor (matched at HLA A, B, C by intermediate resolution typing and HLA-DRB1 by high resolution typing), or unmatched unrelated donor (matched at HLA A, B, C, DRB1 by high resolution typing) will be considered a suitable donor Exclusion Criteria: Patients with peripheral neuropathy greater than grade II Major medical or psychiatric disorders that would seriously compromise patient tolerance of this regimen Human immunodeficiency virus (HIV) infection, active hepatitis B or C infection, or evidence of liver cirrhosis Active viral, bacterial or fungal infection unless adequately treated. For fungal infection, patient should have completed full course of antifungal therapy with resolution of infection. Patients with radiographic changes including pulmonary disease, including but not limited to: pulmonary nodules, infiltrates, pleural effusion are excluded unless cleared by pulmonary biopsy showing no evidence for pulmonary infection Patients with renal insufficiency or cr clearance < 60 ml/min DONOR: Donors will be excluded if for medical or psychological reasons they are unable to tolerate the procedure of peripheral stem cell donation

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Group I (bortezomib, fludarabine phosphate, TMI, melphalan)<br>Patients receive fludarabine phosphate IV on days -9 to -5 and melphalan IV on day -4. Patients also undergo TMI BID on days -9 to -7. If no DLT is observed in the first cohort, bortezomib IV will be added on days -6 and -3 for subsequent cohorts. | Drug: bortezomib<br>* Given IV<br>* Other names: VELCADE;Drug: fludarabine phosphate<br>* Given IV<br>* Other names: Fludara;Other: melphalan<br>* Given IV<br>* Other names: Melfalan;Radiation: total marrow irradiation<br>* Undergo TMI<br>Drug: tacrolimus<br>* Given IV and orally<br>* Other names: Protopic;Drug: sirolimus<br>* Given orally<br>* Other names: SLM;Procedure: peripheral blood stem cell transplantation<br>* Undergo allogeneic PBSC transplant<br>* Other names: transplantation, peripheral blood stem cell;Other: laboratory biomarker analysis<br>* Correlative studies<br>|
| Experimental: Group II (bortezomib, fludarabine phosphate, melphalan<br>Patients receive fludarabine phosphate IV and melphalan IV as in Stratum I. Patients also receive bortezomib IV on days -6, -3, 1, and 4. | Drug: bortezomib<br>* Given IV<br>* Other names: VELCADE;Drug: fludarabine phosphate<br>* Given IV<br>* Other names: Fludara;Other: melphalan<br>* Given IV<br>* Other names: Melfalan;Drug: tacrolimus<br>* Given IV and orally<br>* Other names: Protopic;Drug: sirolimus<br>* Given orally<br>* Other names: SLM;Procedure: peripheral blood stem cell transplantation<br>* Undergo allogeneic PBSC transplant<br>* Other names: transplantation, peripheral blood stem cell;Other: laboratory biomarker analysis<br>* Correlative studies<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Maximum tolerated dose of bortezomib as defined as the highest dose tested in which none or only one patient experiences dose limiting toxicity attributable to the study regimen | Assessed using Common Terminology Criteria for Adverse Events (CTCAE) version 4.0. Dose-limiting toxicity will be defined using Bearman Scale for events that occur. | 6 weeks post transplant | 
| Feasibility of escalating doses of bortezomib with or without TMI in combination with FLU and MEL as preparative regimen for allogeneic hematopoietic stem cell transplant in patients with high risk multiple myeloma |  | 5 years post transplant | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Frequency of clinical response (i.e., complete, partial, or very good partial response) | Response is evaluated based on a new International myeloma working group uniform response criteria. | At 6 months and 1 year post transplant | 
| Frequency of primary and secondary engraftment failure |  | 6 weeks post transplant and 4 years post transplant | 
| Time to neutrophil and platelet engraftment |  | 100 days post transplant | 
| Progression-free survival | Time from day 0 of transplant to the first observation or relapse or death due to any cause, assessed up to 4 years | 4 years post transplant | 
| Incidence of acute and chronic graft-versus-host disease |  | At 6 months and 1 year post transplant up to 4 years post transplant | 
| Overall survival | Time from first day of treatment to time of death due to any cause, assessed up to 4 years | 4 years post transplant | 
| Minimal residue disease | Assessed by flow cytometry | At 6 months and 1 year post transplant |