Efficacy of Wet Cupping on Reactive Oxygen Species and Antioxidants

Study Overview
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Brief Summary
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This study evaluates the efficacy of Wet cupping Therapy on oxidative stress and antioxidant capacity of the body.The healthy volunteers will receive wet cupping application and their initial venous blood samples and samples after the treatment will be evaluated for oxidative stress.

Detailed Description
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Among the healthy individuals who applied for Wet Cupping Therapy (WCT) to Traditional and Complementary Therapies center in Karabuk Medical Faculty Education and Research Hospital for general wellbeing 24 volunteers were enrolled in the study. They will receive three concecutive WCT application in one month interval.Their venous blood samples obtained initially and after the completion of the three concecutive applications, will be evaluted in terms of Reactive Oxygen species and Antioxidant capacity.Additionally samples from the cupping blood during the first and the third WCT application will also be obtained and evaluated.

Official Title
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Efficacy of Wet Cupping on Reactive Oxygen Species and Antioxidant Capacity:A Self-controlled Interventional Study

Conditions
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Healthy

Intervention / Treatment
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* Procedure: Wet Cupping


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy male adults Must apply Traditional and complemantary medicine center for general well being. Exclusion Criteria: Any chronic disorder Antiagregant drug usage Bleeding disorders -

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Wet Cupping<br>One armed self-controlled study. Individuals in this arm will receive three concecutive WCT application | Procedure: Wet Cupping<br>* CT was applied using plastic disposable vacuum cups on the back in the 5 areas of C7 cervical spine (DU14 acupoint), T2-4 lateral spine bilaterally (BL41-42 acupoint) and T6-8 lateral spine bilaterally (BL44-46), which are the recommended sites for headache (12). The cupping technique procedure was conducted in five phases:Primary suction,Area disinfection,Scarification,Secondary suction and bloodletting,Removing and dressing.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| TAS | Total antioxidant status | 7 days | 
| TOS | Total oxidant status | 7 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| SOD | Super oxide dismutase | 7 days | 
| CAT | Catalase | 7 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Wet cupping therapy, antioxidant capacity