Correlation Between Oxidative Stress Status and COVID-19 Severity

Study Overview
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Brief Summary
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During the new COVID-19 pandemic physicians all over the world have faced different challenges .Oxidative stress is a probable cause of multi organ failure in this setting which never has been evaluated in COVID-19 infection to the best of knowledge.Present study aimed to evaluate oxidative stress marker and redox system status in different COVID-19 patients regarding the severity of involvement.

Detailed Description
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Oxidative stress is a probable cause of multi organ failure in this setting which never has been evaluated in COVID-19 infection to the best of knowledge.Present study aimed to evaluate oxidative stress marker and redox system status in different COVID-19 patients regarding the severity of involvement. Demographic and lab data of 60 COVID-19 confirmed positive cases gathered .These patients divided in to two arms: 1- severe COVID-19 which were intubated or admitted in intensive care unit due to severe hypoxia 2- non-severe COVID-19 patients which were not hypoxic and admitted in ward with no need to invasive oxygenation. All patients had positive RT-PCR positive nasopharyngeal swab results for COVID-19 or Lung CT scan compatible with COVID-19. Malondialdehyde(MDA) , Biopterin, Neopterin, SOD, Glutathione levels would be checked by ELISA tests and compared between these two groups and healthy controls.

Official Title
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Correlation Between Oxidative Stress Markers and COVID-19 Severity Index

Conditions
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Oxidative Stress

Intervention / Treatment
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* Diagnostic Test: Oxidative Stress ELISA Kit


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: more than 18 years old positive RT-PCR COVID-19 compatible lung CT with COVID-19 Exclusion Criteria: not signing informed consent using antioxidants using statins

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Severe COVID<br>patients with positive PCR or compatible CT admitted in ICU or intubated | Diagnostic Test: Oxidative Stress ELISA Kit<br>* Checking oxidative stress markers and redox system status<br>|
| Non-severe COVID-19<br>patients with positive PCR or compatible CT admitted in ward without hypoxia | Diagnostic Test: Oxidative Stress ELISA Kit<br>* Checking oxidative stress markers and redox system status<br>|
| Healthy Controls<br>Healthy controls with negative IgM/IgG for COVID-19 | Diagnostic Test: Oxidative Stress ELISA Kit<br>* Checking oxidative stress markers and redox system status<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| MDA concentration | MDA x 2 ULN in severe COVID | 2 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Biopterin concentration | Difference between severe and controls | 2 weeks | 
| Neopterin rate of increase | Difference between severe and controls | 2 weeks |