Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic Disease

Study Overview
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Brief Summary
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Using a human pangenomic microarray, the researchers established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct groups: allergic rhinitis to dust mite (AR) isolated (n=12), AR associated with bronchial hyperreactivity (n=12), AR associated with asthma (n=14), control (n=14).

Detailed Description
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Context: Asthma is the most frequent chronic disease in childhood. Allergic rhinitis has been described as a risk factor to develop asthma. The objective of the study was to evaluate the contribution of the respiratory epithelium to development of allergic rhinitis and asthma and to identify the molecular mechanisms driving rhinitis toward asthma. Methods: Using a human pangenomic microarray, we established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct children 2 to 18 years of age groups: allergic rhinitis to dust mite (AR) isolated (n=12), AR associated with bronchial hyperreactivity (n=12), AR associated with asthma (n=14), control (n=14).

Official Title
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Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic

Conditions
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Allergic Rhinitis

Intervention / Treatment
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* Procedure: Collection of nasal epithelial cells


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients: Patients between 6 and 17 years old Patients with allergic rhinitis Witness: Patients between 6 and 17 years old Patients without allergic rhinitis and asthma Exclusion Criteria: Rhino-bronchitis infection dated from less than 15 days Patients younger than 6 or older than 17 years

Ages Eligible for Study
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Minimum Age: 6 Years
Maximum Age: 17 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Screening
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: A<br>Collection of nasal epithelial cells by brushing | Procedure: Collection of nasal epithelial cells<br>* Collection of nasal epithelial cells by brushing<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Transcriptome analysis: genes statistically modulated between groups, predictive biomarkers allowing correct classification of groups |  | At the sample time | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Validation with other techniques: Q-PCR |  | At the sample time | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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allergic rhinitis to dust mite, allergic rhinitis associated with bronchial hyperreactivity, allergic rhinitis associated with asthma