Influence of Preconceptional Obesity on the Myometrial Cells Differentiation Toward a Contractyl Phenotype

Study Overview
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Brief Summary
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The purpose of this study is to improve the understanding of cellular mecanism involved in delivery disorders in obese women, first by characterizing the hyperplasia phenomenon of myometrail cells induced by inflammatory stimulation (Il-1B), then by studying the role of blood mononuclear cells, of leptine and of obese women sera to interfere with the transition of myometrial cells toward hyperplasia phenotype.

Official Title
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Study of Role of Mononuclear Cells of Maternal Peripheric Blood in Human Myometrial Primar Cell Lines Differentiation Toward a Contratyl Phenotype: Influence of Preconceptional Obesity

Conditions
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Pregnancy Complication

Intervention / Treatment
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* Other: Myometrium biopsy and blood samples


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Informed consent signed Patient affiliated with a social security scheme In front of for obstetrical reasons to give birth by caesarean section (scheduled caesarean section) Or a woman who is not obese in labour and needs to undergo a cesarean section No obvious infectious context Age > 18 years old Exclusion Criteria: Obvious inflammatory or autoimmune diseases Suspected or confirmed infectious context, including virale infection VIH, VHC, VHB Coagulation disorders

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: pre-conceptional obesity with a scheduled cesarean section<br> | Other: Myometrium biopsy and blood samples<br>* The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.<br>|
| Active Comparator: non-obese women with a scheduled cesarean section<br> | Other: Myometrium biopsy and blood samples<br>* The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.<br>|
| Active Comparator: women entering labour<br>women entering labour for vaginal delivery and for vaginal delivery | Other: Myometrium biopsy and blood samples<br>* The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measuring the expression level of markers (Gama actine, Desmine, Connexine-43, and Ocytocine receptor) by Westernblotting | Demonstrate in vitro the capacity of a Il-1B stimulus to induce the differentiation of the myometrial cells toward a hyperplasia phenotype, and that the answer depends on whether this is cells from normal weight women or obese women before conception. | up to 2 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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pregnancy, obesity, differentiation