Biomarkers of Ovarian Reserve and Correlation With IVF Treatments

Study Overview
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Brief Summary
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Retrospective study to evaluate the correlation between AFC, FSHb, AMH and IVF outcomes of 347 cycles performed with three different protocols.

Detailed Description
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347 IVF cycles (236 patients) analyzed for a retrospective study to evaluate the correlation between the biomarkers of ovarian reserve (antral follicles count, antimullerian hormone, basal follicle stimulating hormone) and number of oocytes and mature oocytes (MII) retrieved during the pick up. For the stimulation were used three different protocols: long luteal, antagonist with daily administration of gonadotropins and antagonist with depot administration of gonadotropins.

Official Title
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Predictors of Ovarian Response in Women Undergoing Ovarian Stimulation for IVF

Conditions
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Pregnancy, Ovarian

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: women with primary or secondary infertility underwent ovarian stimulation for in vitro fertilization. Exclusion Criteria: women who underwent ovarian stimulation for cryopreservation in the presence of neoplastic disease.
Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Long luteal protocol<br>Subcutaneous administration of GnRH agonist (Triptorelina pamoato 0.1 mg/ml) in the medium-luteal phase (21th day) of the cycle before the stimulation. The next menstruation, in the presence of estradiol level (E2)<30pg/ml and in the absence of follicular cysts, the gonadotropin stimulation was begun. In the presence of at least three follicles of diameter ≥18mm was induced final oocyte maturation by administration of human chorionic gonadotropin (hCG). | |
| Daily antagonist protocol<br>GnRH antagonist (cetrorelix acetate 0.25 mg) was administered subcutaneously with a fixed protocol from the day +6 of stimulation or with a flexible protocol from the day in which at least one follicle reached a diameter of 14mm. In both protocols GnRH antagonist was administered until the day of the assumption of hCG. Recombinant Follicle Stimulating Hormone (rFSH) was administered from the 3rd day of menstruation at a dose of 150-300 IU/die according to the characteristics of women. The hCG was administered when at least three follicles had reached 18 mm in diameter and estradiol levels were higher than 150 pg/mL/dominant follicle. | |
| Depot antagonist protocol<br>GnRH antagonist (Cetrorelix acetate 0.25 mg) was administered subcutaneously with a fixed or flexible protocol until the day of the assumption of hCG. Corifollitropila alfa was used at dose of 100μg (in women with weight ≤60 kg and age ≤36 years) or 150μg (in women with weight> 60 kg of any age or weight ≥50 kg and age greater than 36 years). Corifollitropina alfa was administered subcutaneously in the day +3 and in the day +5 or +6 was supplemented with rFSH at doses 112-300 IU/day. The hCG was administered when at least three follicles had reached 18 mm in diameter and estradiol levels were higher than 150 pg/mL/dominant follicle. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| number of total and mature (MII) oocytes | the number of total and mature (MII) oocytes retrieved during the pick up. | 10 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| duration of stimulation | the number of days since the administration of rFSH o corifollitropina alfa until the administration of hCG | 10 months | 
| ovarian sensitivity index or OSI | OSI was calculated dividing the total administered FSH dose by the number of retrieved oocytes | 10 months | 
| pregnancy rate | the number of positive pregnancy tests with the evidence of an embyo with cardiac activity. | 10 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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mature oocytes, antral follicles count, follicle stimulating hormone, antimullerian hormone