Serum Folic Acid Status, Genotyping MTHFR 677C-->T, Plasma Homocysteine, DNA Methylation of IGF2 Gene and Microbiota of the Children: Mother-Children Cohort Study

Study Overview
=================
Brief Summary
-----------------
This study is part of the East Jakarta Cohort Study to assess the current micronutrient status of the mothers and their children. The study also includes the assessment of genotyping of MTHFR 677C-->T of the mother and DNA methylation of IGF2 gene of the children.

Detailed Description
-----------------
The initial study recruited 315 mothers in the public health centre and one district hospital in East Jakarta. The follow-up study is able to re-visit the 127 of them. This also includes 127 peers of the subject that lives in the same neighbourhood. The design of the study is case-cohort design, part study of the existing cohort. The assessment of the follow-up study includes the mother and the children born in the initial study. Anthropometric measurement, socioeconomic data from a structured questionnaire, biomarker assessment, blood pressure were obtained from the subjects.

Official Title
-----------------
Serum Folic Acid Status, Genotyping MTHFR 677C-->T of the Mother and Serum Folic Acid Status, DNA Methylation of IGF2 Gene of the Children: East Jakarta Cohort Study

Conditions
-----------------
Folic Acid Deficiency

Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: participant in the previous study apparently healthy data complete voluntarily to participate Exclusion Criteria: not complete data migrate refuse to participate in the study

Ages Eligible for Study
-----------------
Minimum Age: 3 Years
Maximum Age: 40 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
No

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| East Jakarta Cohort Study<br>One group included in the study was originated from mothers who participated in the first data collection pregnancy. The follow-up study included mothers and their children born as the subject in the follow-up in the study. This study also includes peer of the subjects that lived in the same neighbourhood since the first cohort study and have children in the same age with cohort participant | |

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serum folic acid status | The measurement of serum folic acid status is using LC-MS/MS | December 2018 - March 2019 | 
| Genotyping MTHFR 677C-->T | The assessment of MTHFR 677C-->T using Real Time PCR | December 2018 - March 2019 | 
| DNA methylation of IGF2 gene | DNA methylation of IGF2 gene using pyrosequencing method | December 2018 - March 2019 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serum Homocysteine | The measurement of serum homocysteine using Electrochemiluminescen | December 2018-March 2019 | 
| Serum Adiponectin | The measurement of serum adiponection using electrochemiluminescence | December 2018-March 2019 | 
| Blood pressure | The blood pressure measurement using semi automatic device (Omron HBP1300) | December 2018-March 2019 | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
serum folic acid, genotyping, dna methylation of IGF2, East Jakarta Cohort Study, mother-children