Gut Hormone Secretion and Macronutrients

Study Overview
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Brief Summary
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The role of gut hormone (GLP-1, GIP, PYY...) in glucose homeostasis has been widely demonstrated. Furthermore modifications in their pattern of secretion seem to be involved in the improvement of glucose control in obese patients after bariatric surgery. The purpose of this study is to assess the respective role of carbohydrate, fat and protein from a complete meal in gut hormones secretion.

Detailed Description
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The study is designed to asses the role of each individual macronutrient of a compete meal in gut hormones secretion in healthy volunteers. 8 healthy young male subjects will be studied on 5 different occasions. To asses the role of each macronutient the subjects will recieve one of the following test meal: a complete sandwich, the proteic part of this complete meal, the glucidic part and the lipidic part. The fifth test will be a fasting test. To assess the entero-insular axis, a 5.5 hours hyperglycaemic clamp will be performed.

Official Title
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Gut Hormone Secretion Pattern in Response to Different Macronutrients in Healthy Volunteers

Conditions
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Healthy Volunteers

Intervention / Treatment
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* Other: test meal
* Other: glucose
* Other: fat
* Other: protein
* Other: control


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male 18-35 years old 19 kg/m2 ≤ BMI ≤ 25 kg/m2 Healthy Low to moderate physical activity Non smokers Exclusion Criteria: Family history of diabetes mellitus Any medication Participation to blood spending or other clinical study in the 3 months before the beginning of this study Consumption of drugs Consumption of more than 50g of alcool in a week Vegetarian or particular diet Anamnèse d'intolérance alimentaire Family history of food intolerance

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| 1<br>Healthy volunteers | Other: test meal<br>* Mixed meal containing bread, butter, beef<br>Other: glucose<br>* administration of a glucose meal<br>Other: fat<br>* administration of a lipid meal<br>Other: protein<br>* administration of a protein meal<br>Other: control<br>* no meal<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Gut hormone secretion |  | within 4 hours following a test meal | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Entero-insular axis measurement |  | within 4 hours following a test meal | 
| Glucose turnover |  | 6 hours | 
| Insulin sensitivity |  | 6 hours | 
| Substrate oxidation |  | 6 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Gut hormone, hyperglycaemic clamp, glucose turnver