Mitochondrial Oxygraphy on Subcutaneous and Peri-visceral Adipose Tissue: Influence of Body Composition

Study Overview
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Brief Summary
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The objective of this study is to adapt the oxygraphy technique on human adipocytes and to characterize respiration measurements according to patients' body mass index and white adipose tissue localization (subcutaneous vs visceral).

Detailed Description
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During digestive surgery: oncological and bariatric surgery, it is possible to collect a small volume of subcutaneous and epiplonic adipose tissue (10-20 cc). We wish to collect these samples in order to develop the technique of oxygenography on permeabilized adipocytes. In order to characterize these measurements, they will be related to the clinical data of the patients usually collected in the framework of the preoperative assessment including the body mass index (BMI). The measured adipocyte data will thus be normalized to three BMI classes: ≤ 20, > to 20 < 30, and ≥ to 30 kg∙m-2. Primary end point is to validate the measurement of mitochondrial respiration under oxidophosphorylating conditions by determining indicators of the distribution of mitochondrial respiration values (mean, standard deviation, skewness, kurtosis, coefficient of variation) as a function of patients' body mass index.

Official Title
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Mitochondrial Oxygraphy on Subcutaneous and Peri-visceral Adipose Tissue: Influence of Body Composition

Conditions
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Digestive Organs--Cancer, Bariatric Surgery Candidate, Parietal Region Trauma

Intervention / Treatment
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* Procedure: adipose surgery


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients undergoing oncological, bariatric, parietal surgery Patients able to give an informed consent Patients affiliated to an Health Care insurance Patients aged over 18 Exclusion Criteria: Patients under guardianship, curatorship or deprived of freedom Pregnant or breastfeeding women Participation refusal Patients under chemotherapy Patients with peritonitis and infectious parietal processes

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Health Services Research
Intervention Model: Single Group Assignment
Interventional Model Description: all participants receive the same intervention
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: All patients<br>a sample of adipose tissue will be collected during the scheduled surgery | Procedure: adipose surgery<br>* A quantity of adipose tissue equivalent to 10-20 cubic centimeter will be removed subcutaneously and from the omentum during surgical procedures (oncological, bariatric)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Evaluation of mitochondrial respiration on adipose tissue by oxygraphy | Mitochondrial respiration measurements in oxydophosphorylated conditions. For each patient measurements using ADP and carbohydrate or lipid substrates of the respiratory chain complexes for both the subcutaneous and the visceral tissue. | day 1 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| mitochondrial respiration values depending on patients BMI by oxygraphy | Identification of mitochondrial respiration discrimination factors | day 1 | 
| comparing mitochondrial respiration values depending on tissue location by oxygraphy | Identification of mitochondrial respiration discrimination factors | day 1 | 
| comparing mitochondrial respiration values depending on anthropometric data by oxygraphy | Identification of mitochondrial respiration discrimination factors | day 1 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Mitochondria, Oxygraphy, Adipose Tissue, Body Mass Index, Digestive surgery