Prevalence and Risk Factors for Coxiella Burnetii Seropositivity (Q Fever) Among Adults in Western France

Study Overview
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Brief Summary
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Diseases naturally transmitted between animals and humans (zoonoses) are one of the leading causes of emergence or re-emergence of human infectious diseases. Non-foodborne zoonoses are commonly transmitted to human directly by contact with infected animals or contaminated environment. People working with animals such as farmers and veterinarians are particularly at risk of infection. Some zoonoses can also be transmitted indirectly thought the air and therefore can affect the general population. Example is Q fever, a disease caused by Coxiella burnetii, a bacterium highly resistant in the environment. In Western France, Q fever is endemic in cattle herds. To improve human zoonotic disease surveillance, the investigators will conduct a population based study in this area. The aims of the study are: To assess seroprevalence of Coxiella burnetii infection among general population, farmers and veterinarians in Western France To identify risk factors for Coxiella burnetii seropositivity in these populations

Detailed Description
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In order to estimate seroprevalence and risk factors for Coxiella burnetii infection in humans, the investigators will conduct a cross-sectional study in three groups: blood donors at blood donation organizations (general population) beef and dairy cattle farmers veterinarians involved in a rural practice All the participants in the study will provide a blood sample and will fill in a questionnaire.

Official Title
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Prevalence and Risk Factors for Coxiella Burnetii Seropositivity (Q Fever) Among Adults in Western France

Conditions
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Q Fever

Intervention / Treatment
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* Other: blood test


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Give a written informed consent Be 18 years old and over Be able to speak French Exclusion Criteria: Adults under protection (guardianship or curators) Contraindication for drawing blood

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| subjects<br>Data collected on general population, farmers and veterinarians though questionnaires and blood samples. | Other: blood test<br>* Data collected on general population, farmers and veterinarians though questionnaires and blood samples.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Antibody titers against Coxiella burnetii in general population in Western France | Blood samples will be taken on blood donors for detection of Coxiella burnetii antibodies by indirect immunofluorescence assay. | Day 0 | 
| Antibody titers against Coxiella burnetii in farmers population in Western France | Blood samples will be taken on farmers for detection of Coxiella burnetii antibodies by indirect immunofluorescence assay. | Day 0 | 
| Antibody titers against Coxiella burnetii in veterinarians population in Western France | Blood samples will be taken on veterinarians for detection of Coxiella burnetii antibodies by indirect immunofluorescence assay. | Day 0 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measure of association between Coxiella burnetii seropositivity risk and socio-demographic | A short questionnaire will be filled in by participants (general population) to collect socio-demographic, lifestyle and occupational factors. | Day 0 | 
| Measure of association between Coxiella burnetii seropositivity risk and lifestyle | A short questionnaire will be filled in by participants (farmers) to collect socio-demographic, lifestyle and occupational factors. | Day 0 | 
| Measure of association between Coxiella burnetii seropositivity risk and occupational factors. | A short questionnaire will be filled in by participants (veterinarians) to collect socio-demographic, lifestyle and occupational factors. | Day 0 | 
| Measure of association between Coxiella burnetii seropositivity in humans (general population) and cattle herds' prevalence of Coxiella burnetii infection. | Besides blood samples taken on participants, blood samples and milk samples will be taken on beef and dairy cattle herds, respectively, to determine the prevalence of Coxiella burnetii infection in cattle herds. | Day 0 | 
| Measure of association between Coxiella burnetii seropositivity in humans (farmers) and cattle herds' prevalence of Coxiella burnetii infection. | Besides blood samples taken on participants, blood samples and milk samples will be taken on beef and dairy cattle herds, respectively, to determine the prevalence of Coxiella burnetii infection in cattle herds. | Day 0 | 
| Measure of association between Coxiella burnetii seropositivity in humans ( veterinarians) and cattle herds' prevalence of Coxiella burnetii infection. | Besides blood samples taken on participants, blood samples and milk samples will be taken on beef and dairy cattle herds, respectively, to determine the prevalence of Coxiella burnetii infection in cattle herds. | Day 0 |