Mycoprotein and Pea Protein Blend and Muscle Protein Synthetic Response

Study Overview
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Brief Summary
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Dietary protein is vital for the preservation of health and optimal adaptation to training. Plant proteins are considered inferior to animal proteins with respect to their ability to stimulate an acute muscle building response and therefore support long-term muscle reconditioning. Pea protein is a highly commercially available plant proteins source (available as supplements, food ingredients etc.), yet there is no research investigating its ability to stimulate a muscle building response. The investigators aim to assess the effect of consuming pea protein on muscle protein synthesis rates and compare these results to mycoprotein, a source known to elicit a robust anabolic response. Pea protein is lower in some of the essential amino acids, namely methionine, which could mean it is less effective compared with mycoprotein which has a more complete amino acid profile. So in addition to comparing pea with mycoprotein, the investigators also want to compare to a blend of pea and mycoprotein to see if replenishing the amino acid content in pea 'rescues' the anabolic response.

Official Title
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Comparing Postprandial Muscle Protein Synthesis Rates Following the Consumption of Mycoprotein, Pea Protein and a Mycoprotein/Pea Protein Blend in Resistance Trained Individuals

Conditions
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Muscle Protein Synthesis

Intervention / Treatment
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* Dietary Supplement: Bolus ingestion of protein


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI between 18 and 30 Resistance trained Exclusion Criteria: Any metabolic impairment Smoking Use of over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices). A personal family history of epilepsy, seizures or schizophrenia. Allergic to Quorn/mycoprotein/edible fungi/environmental mould products. Any motor disorder.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Mycoprotein<br>Bolus ingestion of mycoprotein providing 25g of protein. | Dietary Supplement: Bolus ingestion of protein<br>* Ingestion of 25g of protein<br>* Other names: Protein ingestion;|
| Experimental: Pea protein<br>Bolus ingestion of pea protein providing 25g of protein. | Dietary Supplement: Bolus ingestion of protein<br>* Ingestion of 25g of protein<br>* Other names: Protein ingestion;|
| Experimental: Mycoprotein/pea protein dry blend<br>Bolus ingestion of mycoprotein/pea protein dry blend providing 25g of protein. | Dietary Supplement: Bolus ingestion of protein<br>* Ingestion of 25g of protein<br>* Other names: Protein ingestion;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein synthesis | The rate of synthesis of new muscle protein (Fractional synthetic rate %/h) | 8.5 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma amino acid kinetics | The appearance of amino acids from the protein drink in the circulation. | 8.5 hours | 
| Serum insulin concentrations | Basal and postprandial concentrations of serum insulin | 8.5 hours | 
| Translocation of mTOR via immunohistochemistry | Locality of mTOR within the muscle cell | 8.5 hours | 
| mTOR phosphorylation via ATP kinase assay | The amount of mTOR that has been activated in the muscle cell | 8.5 hours |