Studying Genes in Samples From Younger Patients With Ovarian or Testicular Sex Cord Stromal Tumors

Study Overview
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Brief Summary
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RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors identify biomarkers related to cancer. PURPOSE: This research trial is studying genes in tumor samples from younger patients with ovarian or testicular sex cord stromal tumors.

Detailed Description
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OBJECTIVES: To assess the role of DICER1 mutations and miRNA pathways in the etiology and progression of ovarian or testicular sex cord stromal tumors. OUTLINE: Archived tumor tissue samples are analyzed for D1CER1 mutation and mRNA expression by reverse-transcriptase polymerase chain reaction (RT-PCR) and laser micro dissector. Results are then correlated with medical data, including age at diagnosis, diagnosis, pathology report, presenting symptoms, family history, treatment, outcome, and length of follow-up, when available.

Official Title
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DICER1 Mutations and miRNA in Ovarian and Testicular Sex Cord Stromal Tumors of Childhood

Conditions
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Childhood Germ Cell Tumor, Leydig Cell Tumor, Ovarian Cancer

Intervention / Treatment
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* Genetic: RNA analysis
* Genetic: gene expression analysis
* Genetic: mutation analysis
* Genetic: reverse transcriptase-polymerase chain reaction
* Other: laboratory biomarker analysis
* Other: medical chart review


Participation Criteria
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Eligibility Criteria
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DISEASE CHARACTERISTICS: Fixed and frozen tissue samples from the ATBR01 B1 tissue bank and from the International Pleuropulmonary Blastoma Registry, Children's Hospital of Boston, and Massachusetts General Hospital Tissue samples from patients diagnosed with: Ovarian Sertoli-Leydig cell tumor Juvenile granulosa cell tumor Adult granulosa cell tumor Gynandroblastoma Sex cord stromal tumor with annular tubules Sex cord stromal tumor, undifferentiated Sertoli tumor Leydig tumor Testicular: Granulosa cell tumor Sertoli tumor Leydig tumor Sertoli-Leydig Undifferentiated stromal tumor PATIENT CHARACTERISTICS: Not specified PRIOR CONCURRENT THERAPY: Not specified

Ages Eligible for Study
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Maximum Age: 120 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Genetic: RNA analysis|nan|
|Genetic: gene expression analysis|nan|
|Genetic: mutation analysis|nan|
|Genetic: reverse transcriptase-polymerase chain reaction|nan|
|Other: laboratory biomarker analysis|nan|
|Other: medical chart review|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Correlation between DICER1 mutations and miRNA pathways with outcomes using descriptive analysis |  |  | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Leydig cell tumor, ovarian stromal cancer, childhood malignant testicular germ cell tumor, childhood malignant ovarian germ cell tumor