Polar Body Removal Before ICSI for Oocyte Activation in Previous Fertilization Failure Cases

Study Overview
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Brief Summary
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Cases which undergone ICSI before with failure of fertilization will do another ICSI cycle with removal of the polar body just before the ICSI procedure and sperm injection at the site of polar body removal. This study group will be evaluated with another group with the classic ICSI procedure

Detailed Description
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Patients with previous fertilization failure will be divided into 2 groups: Group 1 (control): Will undergo classic ICSI procedure in half the MII oocyte of the same case. Group 2 (study group): The other half of the MII oocyte of the same case will undergo: Polar body removal. ICSI procedure with injection of the spermatozoon in the same zona opening (done by laser for polar body removal). Results: Will be collected with evaluation of number of 2PN zygotes, Day 1, Day 2, Day 3, Day 5 embryos in all the cases.

Official Title
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Polar Body Removal Before ICSI as a Method of Oocyte Activation in Previous Fertilization Failure Cases

Conditions
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Fertilization

Intervention / Treatment
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* Procedure: polar body removal using zona drilling by laser then suction by injecting pipette
* Procedure: Classic ICSI


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: previous fertilization failure previous fertilization arrest Exclusion Criteria: NO MII oocyte Refusal of the procedure by the couple

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: polar body removal<br>polar body removal by zona drilling with laser then suction by injecting pipette then ICSI procedure is done | Procedure: polar body removal using zona drilling by laser then suction by injecting pipette<br>* zona drilling with laser followed by polar body removal then ICSI procedure<br>Procedure: Classic ICSI<br>* Classic ICSI case was done be injecting sperm inside Oocyte while polar body is already existed at 6 or 12 O'clock position.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| fertilization rate of the zygotes | fertilization check is done under inverted microscopy by 2PN check | 16-20 hours post ICSI | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| division rate of the embryos by the number of the blastomeres. | Detection of the number of the blastomeres and quality of the embryos under inverted microscopy. At day 5, detection of the rate of the blastocyst formation and quality of the embryos. | Day 2 & 3 & 5 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Polar body removal, ICSI, Fertilization failure