Cause and Importance of the Age-dependent Sarcopenia

Study Overview
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Brief Summary
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The goal of this cross-sectional study is to evaluate the difference in cytokine profiling approach and mitochondrial function between geriatric sarcopenic patients (group 1) and geriatric non-sarcopenic patients (group 2) and healthy (group 3) geriatric participants.

Detailed Description
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Study participants (group 1&2) are assessed during the hospital stay and age-matched controls (group 3) will be recruited through Flyers. The investigators aim to isolate and cryopreserve peripheral blood mononuclear cells (PBMCs) from whole blood to perform functional assays on mitochondrial respiratory capacity and ROS. Furthermore, the investigators will investigate the association between biomarkers and frailty, quality of life, functional performance, fatigue and risk of depression. The investigators hypothesize that this profiling approach will allow the evaluation of the progression of age-dependent sarcopenia and uncover novel therapeutic targets.

Official Title
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Cause and Importance of the Age-dependent Sarcopenia With a Blood Based Bioenergetics & Cytokine Profiling Approach

Conditions
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Sarcopenia

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age between 60 and 85 years Informed written consent Normal cognitive status according to Mini-Mental State Examination (MMSE ≥ 24 points) Life expectancy of > 3 months according to treating doctor without implanted Defibrillation without acute Sepsis without neurodegenerative diseases, especially amyotrophic lateral sclerosis (ALS) and Huntington Exclusion Criteria: Age < 60 years or > 85 years Lack of informed written consent Low cognitive status according to Mini-Mental State Examination (MMSE < 24 points) Life expectancy of < 3 months according to treating doctor Implanted Defibrillation Acute Sepsis neurodegenerative diseases, especially amyotrophic lateral sclerosis (ALS) and Huntington

Ages Eligible for Study
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Minimum Age: 60 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group 1<br>Geriatric patient with Sarcopenia | |
| Group 2<br>Geriatric Patient without Sarcopenia | |
| Group 3<br>healthy control group | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Differences in mitochondrial respiratory capacity of peripheral blood mononuclear cells (PBMCs) using Oxygraph-2k respirometry |  | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Quality of life (validated questionnaire) |  | Baseline | 
| Frailty Status according to Fried et.al. criteria |  | Baseline | 
| Functional limitations (single validated questionnaire) |  | Baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Sarcopenia, Geriatrics, Biomarker