Westlake Longevity Cohort

Study Overview
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Brief Summary
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The Westlake Longevity Cohort (We-Longevity) is a prospective cohort study among centenarians, nonagenarians, senior citizens and their family members up to three generations living in Lishui, China. The primary aim of this cohort is to characterize the multi-omics molecular characteristics of healthy longevity and their dynamic trajectories. Another aim of We-Longevity is to investigate the association of dietary and lifestyle with the multi-omics molecular characteristics of healthy longevity, and to facilitate the development of personalized nutritional/lifestyle recommendation for the public.

Detailed Description
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Aging is a major risk factor for most fatal diseases, such as Alzheimer's disease, type 2 diabetes, cardiovascular disease and cancer. Global population aging is becoming increasingly serious, and this trend is mainly driving the annual increase in the prevalence of aging-related diseases. Healthy longevity are influenced by a variety of factors, such as diet structure, lifestyle, ecological environment, gut microbiome and genetics. At present, the researches on healthy longevity are mainly focused on Drosophila, nematodes and mouse models, and few studies on longevity population. Centenarians and nonagenarians are the best objects to study healthy longevity. However, the multi-omics molecular characteristics and regulatory mechanism of healthy longevity in human are still unclear. There, the present We-Longevity design includes 200 centenarians, 600 nonagenarians, 400 senior citizens. We will determine the fecal/serum metabolome, gut microbiome serum proteome and genome, together with diet, lifestyle and disease information.

Official Title
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Westlake Longevity Cohort

Conditions
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Aging, Alzheimer's Disease, Neurodegenerative Diseases, Cardiometabolic Diseases

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Participants aged 50 years or order. Participants live in Lishui, China. Participants intend to remain in Lishui for ≥3 years. Exclusion Criteria: Participants with cancer, serious medical disorders or infectious diseases.

Ages Eligible for Study
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Minimum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| aging | Physical frailty will be evaluated by using Fried's criteria. | 36 months | 
| Gut microbiota | Fecal microbiome will be analyzed by 16S rRNA gene sequencing and metagenome sequencing. | 36 months | 
| Metabolomics of serum and gut microbiome | Metabolomics of serum and gut microbiome will be analyzed by liquid chromatograph-mass spectrometer (LC/MS). | 36 months | 
| Proteomic profiling of serum | Proteomic characterization of serum will be analyzed by liquid chromatograph-mass spectrometer (LC/MS). | 36 months | 
| Genome | Genomic characterization will be analyzed by Illumina ASA-750K arrays. | 36 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Cognitive function | Cognitive function will be evaluated by using Mini-Mental State Examination (MMSE). | 36 months | 
| Blood glucose examination of blood samples | Blood glucose will be analyzed by automatic biochemical analyser. | 36 months | 
| Blood blood lipids examination of blood samples | Blood blood lipids (HDL, LDL, TC and TG) will be analyzed by automatic biochemical analyser. | 36 months | 
| Serum cytokines examination of blood samples | Serum cytokines (IFNγ, IL10, IL12p70, IL13, IL1β, IL2, IL4, IL6, IL8 and TNFα) will be analyzed by electrochemiluminescence immunoassay. | 36 months | 
| Serum neurotranszmitters examination of blood samples | Serum neurotranszmitters (dopamine, choline, acetyl choline, tyramine, tryptamine, 5-hydroxyindoleacetic acid, phenylpyruvic acid, 3,4-dihydroxyphenylacetic acid and 3-hydroxyo-aminobenzoic acid) will be analyzed by liquid chromatograph-mass spectrometer (LC/MS). | 36 months |