Development of an in Vitro Hematopoietic Culture System and Application to Myelodysplastic Syndromes.

Study Overview
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Brief Summary
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Myelodysplastic syndromes (MDS) are myeloid hemopathies characterized by ineffective clonal haematopoiesis, peripheral cytopenias and a predisposition to the occurrence of acute myeloid leukemias. Their diagnosis involves a cytological evaluation of the medulla, while their prognosis, in addition to extrinsic factors depending on the patient himself (age, comorbidities), intrinsic factors. The cytological evaluation is subject to a certain subjectivity since qualitative and the diagnosis is sometimes difficult in the absence of marker of clonality. More and more studies emphasize the interest of flow cytometry (CMF) in the diagnosis of SMD: by looking for qualitative and / or quantitative aberrations of the expression of membrane markers, CMF allows to establish scores Diagnosis that we have put in place within the laboratory. However, these studies are based on a static model that studies the phenotypic characteristics of patients at a given time but does not really reflect ineffective hematopoiesis. A dynamic model for in vitro reproduction of hematopoiesis would be an innovative tool for the study of SMD. This project aims to develop and standardize a system of differentiation in liquid medium of hematopoietic stem cells (CSH) in mature cells by studying each stage of the differentiation in terms of proliferation, apoptosis and phenotypic expression. HSCs will be obtained by CD34 + sorting from the medullary sample at diagnosis: the investigator will study cell proliferation, apoptosis and the acquisition of surface markers, in order to identify the quantitative and qualitative abnormalities associated with the differentiation of haematopoietic progenitors Smart. This should make it possible to identify diagnostic and prognostic factors in terms of response to treatment, acutism and survival.

Official Title
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Development of an in Vitro Hematopoietic Culture System and Application to Myelodysplastic Syndromes.

Conditions
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Hematopoietic Cell Proliferation, Myelodysplastic Syndromes

Intervention / Treatment
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* Other: Dynamic cultures of in vitro hematopoietic stem cells


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient with diagnosis, major ≥18 years Explained in the blood disease department for cytopenia (s) For which diagnosis of myelodysplastic syndrome is considered Justifying a diagnostic bone marrow (myelogram) Having been informed of the progress of this study by the referring physician of the patient during the consultation and having expressed their non-opposition Exclusion Criteria: High-grade myelodysplastic syndromes on IPSS score (intermediate -2 or more) on preliminary cytological analysis Acute mesoblastic leukemia from the outset

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Diagnostic patient<br> | Other: Dynamic cultures of in vitro hematopoietic stem cells<br>* Improvement of prognostic scores used in myelodysplastic syndromes by identifying by dynamic in vitro culture of hematopoietic stem cells dynamic factors, reflections of ineffective haematopoiesis<br>|
| Control<br> | Other: Dynamic cultures of in vitro hematopoietic stem cells<br>* Improvement of prognostic scores used in myelodysplastic syndromes by identifying by dynamic in vitro culture of hematopoietic stem cells dynamic factors, reflections of ineffective haematopoiesis<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Phenotypic aberration detectable in flow cytometry during in vitro erythroid differentiation of hematopoietic stem cells from patients with MDS |  | 1 day |