Neuro-cognitive Impact of Juvenile Obesity

Study Overview
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Brief Summary
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Recent studies indicate that obese teenagers exhibit memory disturbances. Adolescence represents a crucial period in the development of the hippocampus and the amygdala, necessary for the implementation of memory and emotional functions for the rest of life. Disturbances of the interaction between amygdala and hippocampus during adolescence have been associated with the development of neuropsychiatric disorders. However, the impact of juvenile obesity on functional amygdala-hippocampus connectivity has not been evaluated yet. The main objective of this study is to compare the emotional memory performance and the level of functional connectivity between the hippocampus and the amygdala during the realization of an emotional associative memory task, in obese and control adolescents.

Detailed Description
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Obesity increases the prevalence of cognitive impairment. In particular, it can promote disturbances of contextual memory dependent on the hippocampus. Obesity is also associated with negative emotional consequences. However, no clinical study has so far evaluated the effect of obesity on emotional memory, a function underpinned by the amygdala. Recent studies indicate that obese children and adolescents exhibit memory disturbances. Adolescence represents a crucial period in the development of the hippocampus and the amygdala, two brain regions implicated in memory and emotional functions for the rest of life. Changes in connectivity during development, whether structural or functional, are major before the age of 10 and progressive and minor until the age of 30. Disturbances of this amygdala-hippocampal functional connectivity during adolescence have been associated with the development of neuropsychiatric disorders. However, the impact of childhood obesity on functional amygdala-hippocampus connectivity has still not been evaluated. The first aim of the study is to compare the functional connectivity of the hippocampus and the amygdala measured during the realization of an emotional associative memory task or at rest before and after the task between obese teenagers and control teenagers and how closely it is related to emotional memory performances. Secondly, it will be determined whether the level of functional connectivity between the hippocampus and the amygdala is related to cognitive performances and eating symptomatology in obese teenagers.

Official Title
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Impact of Juvenile Obesity on Emotional Memory and Functional Connectivity Between the Amygdala and the Hippocampus

Conditions
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Pediatric Obesity, Behavior and Behavior Mechanisms, Emotions

Intervention / Treatment
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* Behavioral: an MRI examination


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Experimental group: 12-17 years old boys, BMI>30 (according to IOTF), right-handed, French secondary school, beneficiary of social security, consent of the holders of parental authority. Control group: 12-17 years old boys, BMI<25 (according to IOTF), right-handed, French secondary school, beneficiary of social security, consent of the holders of parental authority. Exclusion Criteria: Adolescent with pubertal stage of Tanner <2 Adolescent with known visual or auditory difficulties Adolescent with a known chronic pathology requiring medication Adolescent with psychotic, neurodevelopmental or substance use disorder Adolescent who may have a contraindication to the MRI examination (presence in the body of metallic prostheses, pacemaker, metal chips, claustrophobia).

Ages Eligible for Study
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Minimum Age: 12 Years
Maximum Age: 17 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: experimental group<br>Thirty obese boys (12-17 years old) undergoing an MRI, a clinical and psychological examination and a series of cognitive tests | Behavioral: an MRI examination<br>* The MRI examination implies the realization of an emotional associative memory task<br>* Other names: a clinical and psychological examination and a series of cognitive tests;|
| Other: control group<br>Thirty healthy boys (12-17 years old) paired for pubertal stage, level of education and socio-economic level undergoing an MRI, a clinical and psychological examination and a series of cognitive tests | Behavioral: an MRI examination<br>* The MRI examination implies the realization of an emotional associative memory task<br>* Other names: a clinical and psychological examination and a series of cognitive tests;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| the level of connectivity between the amygdala and the hippocampus recorded during the execution of an emotional memory task in the scanner. | The level of connectivity between the hippocampus and the amygdala during the performance of the task will be measured through the fluctuations of the blood oxygen level-dependent (BOLD) signal obtained in activation functional magnetic resonance imaging (fMRI) during the performance of an associative emotional memory task. The level of functional connectivity between two brain regions will be deduced from the temporal correlation between the fluctuations of the BOLD signal between these two regions. This coefficient will be normalized in Z-score before analysis. | through study completion, an average of 24 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The level of connectivity between the amygdala and the hippocampus before and after the task will also be recorded at rest. | The level of functional connectivity at rest will be measured before and after the task performed in the scanner. The fluctuations of the BOLD signal recorded at rest (while the subject is quietly resting eyes closed in the machine without being engaged in the performance of a task) also makes it possible to measure levels of functional connectivity of rest between two brain regions according to a method a little different from that applied to activation images (after filtering high frequencies). A Z-score reflecting the level of connectivity will be calculated before statistical analysis. | through study completion, an average of 24 months | 
| The score at the emotional memory task performed in the scanner will be collected | The behavioral performance associated with the completion of the task is collected in an e-Prime file and expressed as percentages of correct answers. | through study completion, an average of 24 months | 
| grey matter volumes (mm3) | The investigators will construct volumetric grey matter maps using Voxel-Based-Morphometry toolbox of SPM for each subject so as to conduct whole brain analysis. | through study completion, an average of 24 months | 
| cortical thicknesses (mm) | The investigators will use FreeSurfer Suite to evaluate cortical thickness for each subject. | through study completion, an average of 24 months | 
| The percentage of body fat | The percentage of body fat will be evaluated by a bio-impedancemetry scale that automatically provides a measure of body fat and lean mass. | through study completion, an average of 24 months | 
|  Paired Associates Learning Task  administrated through the CANTAB neuropsychological examination | Paired Associates Learning assesses visual memory and new learning. Administration time: 8 minutes Task format: Boxes are displayed on the screen and are opened in a randomised order. One or more of them will contain a pattern. The patterns are then displayed in the middle of the screen, one at a time and the participant must select the box in which the pattern was originally located. If the participant makes an error, the boxes are opened in sequence again to remind the participant of the locations of the patterns. Increased difficulty levels can be used to test high-functioning, healthy individuals. Outcome measures include the errors made by the participant, the number of trials required to locate the pattern(s) correctly, memory scores and stages completed. | through study completion, an average of 24 months | 
| the number of distinct words for the free-recall phase ( Verbal Recall/Recognition Memory Task  administrated through the CANTAB neuropsychological examination) | Verbal Recognition Memory assesses verbal memory and new learning. It measures the ability to encode and subsequently retrieve verbal information, with recall tapping into fronto-temporal networks and recognition assessing hippocampal areas. Administration time: 10 minutes Task format : The participant is shown a sequence of words on screen one by one. The participant is then tasked with recalling the words, whilst a rater marks which ones they remembered. In the next phase, the participant is presented with two words, one from the original list and one distractor and is asked to choose which one they have seen before, in a 2-force choice paradigm. The latter recognition phase is then repeated after a delay. One of the outcome measures is the number of distinct words for the free-recall phase | through study completion, an average of 24 months | 
|  Verbal Recall/Recognition Memory Task  administrated through the CANTAB neuropsychological examination | Verbal Recognition Memory assesses verbal memory and new learning. It measures the ability to encode and subsequently retrieve verbal information, with recall tapping into fronto-temporal networks and recognition assessing hippocampal areas. Administration time: 10 minutes Task format : The participant is shown a sequence of words on screen one by one. The participant is then tasked with recalling the words, whilst a rater marks which ones they remembered. In the next phase, the participant is presented with two words, one from the original list and one distractor and is asked to choose which one they have seen before, in a 2-force choice paradigm. The latter recognition phase is then repeated after a delay. The other outcome measures is the number of correct and incorrect responses for the immediate and delayed recognition parts of the test. | through study completion, an average of 24 months | 
| Percentage of correct and incorrect responses at the  Emotion Recognition Task  administrated through the CANTAB neuropsychological examination | The Emotion Recognition Task (ERT) measures the ability to identify six basic emotions in facial expressions along a continuum of expression magnitude. Administration time: 6-10 minutes Task format: Computer-morphed images derived from the facial features of real individuals, each showing a specific emotion, are displayed on the screen, one at a time. Each face is displayed for 200ms and then immediately covered up to prevent residual processing of the image. The participant must select which emotion the face displayed from 6 options (sadness, happiness, fear, anger, disgust or surprise). The first outcome measure for ERT is the percentages of correct and incorrect responses | through study completion, an average of 24 months | 
| Number of correct or incorrect responses at the  Emotion Recognition Task  administrated through the CANTAB neuropsychological examination | The Emotion Recognition Task (ERT) measures the ability to identify six basic emotions in facial expressions along a continuum of expression magnitude. Administration time: 6-10 minutes Task format: Computer-morphed images derived from the facial features of real individuals, each showing a specific emotion, are displayed on the screen, one at a time. Each face is displayed for 200ms and then immediately covered up to prevent residual processing of the image. The participant must select which emotion the face displayed from 6 options (sadness, happiness, fear, anger, disgust or surprise). The second outcome is the numbers of correct or incorrect response. | through study completion, an average of 24 months | 
| Response latencies at the  Emotion Recognition Task  administrated through the CANTAB neuropsychological examination | The Emotion Recognition Task (ERT) measures the ability to identify six basic emotions in facial expressions along a continuum of expression magnitude. Administration time: 6-10 minutes Task format: Computer-morphed images derived from the facial features of real individuals, each showing a specific emotion, are displayed on the screen, one at a time. Each face is displayed for 200ms and then immediately covered up to prevent residual processing of the image. The participant must select which emotion the face displayed from 6 options (sadness, happiness, fear, anger, disgust or surprise). The last outcome is the response latencies | through study completion, an average of 24 months | 
| cognitive functioning of the adolescent | The cognitive functioning of the adolescent will also be evaluated by his parents through the raw scores on the 8 subscales of the BRIEF-C and on composite index (Behavioral Regulation Index and Metacognition Index), based on a set of the 8 raw scores. | through study completion, an average of 24 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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obesity, teenagers, emotional memory, brain networks