Study of 5-Fluorouracil/Leucovorin/Oxaliplatin (FOLFOX) + Bevacizumab Versus 5-Fluorouracil/Leucovorin/Oxaliplatin/Irinotecan (FOLFOXIRI) + Bevacizumab as First Line Treatment of Patients With Metastatic Colorectal Cancer Not Previously Treated and With Three or More Circulating Tumoral Cells

Study Overview
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Brief Summary
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The purpose of the study is to evaluate FOLFOX + bevacizumab versus FOLFOXIRI + bevacizumab as first line treatment of patients with metastatic colorectal cancer not previously treated and with three or more circulating tumoral cells.

Official Title
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Phase III, Randomized Clinical Trial to Evaluate FOLFOX + Bevacizumab Versus FOLFOXIRI + Bevacizumab as First Line Treatment of Patients With Metastatic Colorectal Cancer Not Previously Treated and With Three or More Circulating Tumoral Cells.

Conditions
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Metastatic Colorectal Cancer

Intervention / Treatment
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* Drug: modified FOLFOX6 + bevacizumab
* Drug: FOLFOXIRI + Bevacizumab


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient's Informed consent in written. Age between 18 and 70 years old. Eastern Cooperative Oncology group performance status (ECOG) 0-1. Life expectancy of at least 3 months. Histological confirmation of adenocarcinoma of the colon or rectum. To be included in the study patients should present > or equal 3 CTC in peripheral blood. Measurable metastatic stage IV disease with at least 1 measurable metastatic lesion following Response Evaluation Criteria In Solid Tumors (RECIST) criteria v 1.1 (non suitable for radical surgery at the inclusion time). Prior radiotherapy is allowed but must be completed at least 4 weeks before randomization (if applicable). Adequate bone marrow, liver and renal function. Women of childbearing potential must have a negative serum or urine pregnancy test. Postmenopausal women must have been amenorrheic for at least 12 months.Both men and women participating in this study must use adequate contraception. Subject must have the ability, in the opinion of the investigator, to comply with all the study procedures and follow-up examinations. Exclusion Criteria: Previous chemotherapy for metastatic disease. Prior treatment with Bevacizumab, or Epidermal Growth Factor Reception (EGFR) inhibitors Any anticancer treatment (chemotherapy, hormonal treatment, radiation treatment, surgery , immunotherapy, biologic therapy or tumour embolization) within 4 weeks before randomization. Use of any investigational drug within 4 weeks before start the treatment Clinical or radiographic evidence of brain metastasis. Uncontrolled hypertension (systolic blood pressure > 150 mmHg and/or diastolic blood pressure > 100 mmHg on repeated measurement) despite optimal medical management. Previous history of hypertensive encephalopathy or hypertensive crises. Current or history of peripheral neuropathy > or equal to 1 National Cancer Institute-Common Terminology Criteria for Adverse Events (NCI-CTCAE). Patients classified as fragile according to criteria listed in the protocol. Significant cardiovascular disease (e.g. cerebrovascular accident (CVA), myocardial infarction, within 6 months before randomization). Unstable angina, congestive heart failure New York Heart Association (NYHA) ≥ class II, arrhythmia that requires treatment within 3 months before randomization. Significant vascular disease (e.g. aortic aneurism requiring surgical intervention, pulmonary embolic, peripheral arterial thrombosis) within 6 months before randomization. Previous history of significant haemorrhage /severe, within 1 month before randomization. Major surgery, open surgical biopsy or significant traumatic injury within 4 weeks before randomization. Large bore needle biopsy of a major organ within 14 days before randomization. Placement of central venous access port > or equal to 7 days before randomization is permitted Evidence or history of bleeding diathesis or coagulopathy. International Normalized Ratio (INR) > 1.5 within 14 days prior to starting study treatment. EXEMPTION: patients on full anticoagulation must have an in-range INR [usually between 2-3]. Any anticoagulation therapy must be at stable dosing prior to enrollment. History of previous abdominal fistula or gastrointestinal perforation within 6 months before randomization. Serious non-healing wound, ulcer or bone fracture. Acute or sub-acute of intestinal occlusion or history of intestinal inflammatory disease. History of uncontrolled convulsive crises. History of pulmonary fibrosis, acute lung disease or interstitial pneumonia. Chronic, actual o recent use (10 days prior first drug administration) of acetylsalicylic acic (aspirin) > 325 mg/day or clopidogrel (75mg/day) or other treatments that can cause gastrointestinal ulcer (low-dose aspirin is permitted < or equal to 325 mg/day). Urinary protein excretion > or equal to 2+ (dipstick). If > or equal 2 g proteinuria is detected with dipstick, a 24-hour period urine test will be performed and the result should be < or equal to 1 g/24 hours to permit the inclusion of the patient in the clinical trial. Known human immunodeficiency virus infection or chronic hepatitis B or C infection or other uncontrolled, severe concurrent infection . Current infection > or equal to Grade 2 (NCI-CTCAE). Any previous or concurrent cancer different to colorectal carcinoma within 5 years before to start the treatment. Subjects with successfully treated, non-invasive cancers, including cervical cancer in situ, basal cell carcinoma will be allowed to participate in the clinical trial. Or those cancer treated with curative intention without disease evidence in the last 5 years at least. Known or suspected allergy or hypersensitivity to any component of Bevacizumab, oxaliplatin, irinotecan, or 5-FU/LV. Any medical, psychological, or social condition that may interfere with the subject's participation in the study or evaluation of the study results. Any psychological, familial or geographic situation that interferes in the adequate follow.up and adherence to the study protocol. Women who are pregnant or breast-feeding.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Control<br>modified FOLFOX6 + bevacizumab | Drug: modified FOLFOX6 + bevacizumab<br>* Bevacizumab 5 mg/kg iv, day 1, followed by Oxaliplatin 85 mg/m2 iv administered over a period of 2 hours, day 1, followed by 5- Fluorouracil (FU)/Leucovorin (LV), day 1 and 2, as follow: LV 400 mg/m2 iv administered over a period of 2 hours, followed by 5-FU 400 mg/m2 iv bolus, followed by 5-FU 2.400 mg/m2 over 46 h continuous infusion. This treatment will start on day 1 and will be repeated every 2 weeks (1 cycle).<br>|
| Experimental: Experimental<br>FOLFOXIRI+bevacizumab | Drug: FOLFOXIRI + Bevacizumab<br>* Bevacizumab 5 mg/kg iv, followed by Irinotecan 165 mg/m2 iv administered over a period of 30-90 minutes, followed by Oxaliplatin 85 mg/m2 iv administered over a period of 2 hours, followed by LV 400 mg/m2 iv administered over a period of 2 hours, followed by 5-FU 3,200 mg/m2 for 48 h continuous infusion. This treatment will start on day 1 and will be repeated every 2 weeks (1 cycle).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Progression free survival (PFS) |  | 5 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Overall survival (OS) |  | 5 years | 
| Response rate (RR) |  | 5 years | 
| Radical Resection (R0) surgery rate |  | 5 years | 
| Circulating Tumour Cells (CTC) count basal and correlate to PFS, OS, RR |  | 5 years | 
| Correlation of RAS, BRAF and PI3K mutations and clinical anti-tumour activity outcome ( PFS, OS, RR) |  | 5 years | 
| Adverse events |  | 5 years | 
| Correlation of molecular status of bio markers related to the cellular and tumoral reproduction and/or mode of action and clinical anti-tumour activity outcome ( PFS, OS, RR) |  | 5 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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colorectal cancer, FOLFOX, Bevacizumab, FOLFOXIRI, circulating tumor cell