Effect of Red Raspberry Consumption on Fasting Oxidative Stress Biomarkers

Study Overview
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Brief Summary
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The purpose of this study is determine whether daily consumption of frozen red raspberries can improve the antioxidant capacity of the blood and reduce the effects of oxidative stress, dose-dependently, in a healthy adult population.

Detailed Description
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Red raspberries are an excellent source of numerous polyphenolic antioxidants. The bioactivity of these compounds have been implicated in many biological pathways and may function to reduce the risk of numerous chronic diseases. We are conducting an intervention study with frozen red raspberries on a healthy adult population in order to determine if raspberry polyphenols are bioavailable and bioactive in vivo. Twenty-four subjects (12 male and 12 female) will be recruited to participate and consume one-cup frozen red raspberries daily for two weeks, they then will randomly be assigned to consume either one, two or four cups of raspberries for another two weeks, this will be followed by four weeks of non-treatment. Blood samples and 12-hour urine collections will be taken in order to measure the concentration of raspberry polyphenols in response to treatment dose. Dose-dependent treatment effects on blood biomarkers for antioxidant capacity, oxidative stress, inflammation and other chronic disease risk factors will also be measured.

Official Title
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Raspberries and Human Health: A Short-term Intervention Trial Investigating the Effect of Daily Red Raspberry Consumption on Fasting Oxidative Stress Biomarkers

Conditions
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Oxidative Stress

Intervention / Treatment
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* Dietary Supplement: individually quick frozen red raspberries
* Dietary Supplement: individually quick frozen red raspberries
* Dietary Supplement: individually quick frozen red raspberries


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy adults Exclusion Criteria: obese (>30kg/m2) high blood pressure (140/90mmHg) allergic to raspberries pregnant or lactating history of chronic disease currently taking medication

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 43 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: one-cup<br> | Dietary Supplement: individually quick frozen red raspberries<br>* Consumption of one-cup frozen red raspberries daily for four weeks, followed by a four week period of non-treatment.<br>|
| Experimental: two-cups<br> | Dietary Supplement: individually quick frozen red raspberries<br>* Consumption of one-cup frozen red raspberries for two weeks, then consumption of two-cups frozen red raspberries for an additional two weeks, followed by four weeks of non-treatment.<br>|
| Experimental: four-cups<br> | Dietary Supplement: individually quick frozen red raspberries<br>* Consumption of one-cup frozen red raspberries for two weeks, then consumption of four-cups frozen red raspberries for an addition two weeks, followed by four weeks of non-treatment.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| serum TBARS | an indicator of MDA levels, and therefore, lipid peroxidation | end of week 1, 3, 5 and 9 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| serum antioxidant capacity (AOC) | measured using the TEAC-ABTS radical cation assay | end of week 1, 3, 5 and 9 | 
| serum thiols | measured using the DTNB assay, as an indicator of antioxidant levels in protein fraction | end of week 1, 3, 5 and 9 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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raspberry, fruit, antioxidants, polyphenols, oxidative stress, dietary intervention