Coronavirus Pediatric Serological Study

Study Overview
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Brief Summary
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The Coronavirus Pediatric Serological Study's aim is to provide a better insight of children's immune response after SARS-CoV-2 infection. Serological tests were conducted on blood samples taken 3 to 5 months after the children's viral infection. The study was performed in collaboration with the Institute of Medical Immunology, were antibody levels were measured and immunoglobulin subclasses profiles were studied.

Detailed Description
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The hospital laboratory provided us the list of children under 15 years old tested for SARS-CoV-2 in Saint-Pierre Hospital (Brussels) by PCR on nasopharyngeal swab between March 1 2020 and December 31 2020. The medical files of all children that were tested positive for SARS-CoV-2 were reviewed and clinical information was collected. For each positive child, the family was contacted by phone 3 months after the proven viral infection in order to ask permission to take a single blood test. Covid19 serologies were performed in the hospital laboratory, and blood samples were then sent to the Institute of Medical Immunology, were antibody levels and immunoglobulin subclasses were studied.

Official Title
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Coronavirus Pediatric Serological Study

Conditions
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SARS-CoV2 Infection

Intervention / Treatment
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* Diagnostic Test: Blood sample


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Tested positive for SARS-CoV-2 by PCR on naso-pharyngeal swab performed in Saint-Pierre Hospital (Brussels) between March 1 2020 and December 31 2020 Exclusion Criteria: /

Ages Eligible for Study
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Minimum Age: 0 Months
Maximum Age: 179 Months

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Positive Children<br>Children under 15 years old tested positive for SARS-CoV-2 by PCR on nasopharyngeal swab | Diagnostic Test: Blood sample<br>* Blood sample taken 3 to 5 months after the infection<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| SARS-CoV-2 antibody levels in asymptomatic patients | Measurement of SARS-CoV-2 IgG (total and subclasses), IgA (total and subclasses) and IgM levels in the sera of children who did not show any symptoms at the time of the viral infection | 3 to 5 months after SARS-CoV-2 infection | 
| SARS-CoV-2 antibody levels in mildly symptomatic patients | Measurement of SARS-CoV-2 IgG (total and subclasses), IgA (total and subclasses) and IgM levels in the sera of children presenting mild symptoms at the time of the viral infection | 3 to 5 months after SARS-CoV-2 infection | 
| SARS-CoV-2 antibody levels in hospitalized patients | Measurement of SARS-CoV-2 IgG (total and subclasses), IgA (total and subclasses) and IgM levels in the sera of children who required an hospitalisation at the time of the viral infection | 3 to 5 months after SARS-CoV-2 infection | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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SARS-CoV-2, Immune response, Children