Influence of Propofol on Alveolar Macrophage in Asthmatic Patients

Study Overview
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Brief Summary
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The change of airway responsiveness and alveolar macrophage (AM) immunity occurs during the pathogenesis of asthma. Macrophage-associated airway protection in patients with asthma is affected positively or negatively by propofol general anesthesia. The investigators hypothesized that the effects of propofol on asthmatic AMs and corresponding airway reactivity in vivo is different from the in vitro research. Through in vivo airway hyperresponsiveness (AHR) observation and clinical investigation of AM immune functions from asthma patients undergoing propofol general anesthesia to clarify the precise effects of propofol on AM immunity, and to provide theoretical basis for the clinical administration of propofol in asthmatic patients. This would optimize the practice of clinical anesthesia, especially for those with the tendency of AHR.

Official Title
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Influence of Propofol on Alveolar Macrophage Immunity in Asthmatic Patients Undergoing General Anesthesia

Conditions
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Asthma

Intervention / Treatment
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* Drug: 2,6-Diisopropylphenol


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: ASA physical status I-II; Aged from 18 years to 45 years; Patients with or without asthma or a history record of asthma; Undergoing elective lumpectomy under general anesthesia. Exclusion Criteria: Allergy to propofol and opioids, a history of the use of centrally-acting drugs of any sort, chronic pain and psychiatric diseases records; Participants younger than 18yr，older than 45yr or pregnancy; Those who were not willing to or could not finish the whole study at any time; The PACU assessing score was under 6 on a scale of 10 (measuring somnolence, respiration, movement, color, and blood pressure on 0-2 scales), and arterial oxygen saturation measured by pulse oximetry (SaO2) was 92% or lower (supplemental oxygen was permitted).

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| 1<br>Propofol general anesthesia for asthmatic patients | Drug: 2,6-Diisopropylphenol<br>* Propofol general anesthesia was performed in patients with asthma (1) and without asthma (2)<br>* Other names: Diprivan;|
| 2<br>Propofol general anesthesia for non-asthmatic patients | Drug: 2,6-Diisopropylphenol<br>* Propofol general anesthesia was performed in patients with asthma (1) and without asthma (2)<br>* Other names: Diprivan;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Alveolar macrophage viability assay |  | One day prior to the surgery, immediately after anaesthesia, at the beginning and the end of the operation and one day after surgeries. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Alveolar macrophage phagocytosis Assay |  | One day prior to the surgery, immediately after anaesthesia, at the beginning and the end of the operation and one day after surgeries. | 
| Alveolar macrophage cytokine Assay |  | One day before the surgery, immediately after anaesthesia, at the beginning and the end of the operation and one day after surgeries. | 
| Mean airway pressure |  | Before operation to the end of the surgery. | 
| Curve shape of CO2 |  | Before operation to the end of the surgery. | 
| End-tidal CO2 |  | Before operation to the end of the surgery. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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General anesthesia, Propofol, Immunity, Alveolar Macrophages