The Effect of a Plant Protein Isolate on Muscle Protein Synthesis in Humans at Rest and After Resistance Exercise

Study Overview
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Brief Summary
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Plant-based proteins contain several amino acids that are essential to signal growth, reconditioning, maintenance and preservation of skeletal muscle mass. This study aims to inform on the nutrient activation of muscle protein synthesis (MPS) by feeding a plant protein isolate equivalent to 0.33 g of protein per kilogram of body mass.

Detailed Description
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Participants: 32 young, healthy men and women aged 18 - 35 years. Intervention: Plant protein isolate (PPI) and nonessential amino acid control (NEAA) Study Design: Block randomised control trial. Two group parallel design. Primary outcome: Myofibrillar fractional synthetic rate (myoFSR). Preliminary screening/familiarization consists of: medical history, dietary assessment and examination by a medical doctor and a qualified dietician, blood sample to be evaluated for health-related contraindications, body composition measurement by dual energy x-ray absorptiometry (DXA), isokinetic knee extensor strength tests. Pre-trial: A metered dose of deuterium oxide (5ml/kg) will be ingested and blood and/or saliva ( 5ml) samples will be collected pre/post ingestion. Experimental trial: Blood, saliva and bilateral m.vastus lateralis microbiopsies ( 100 mg) collected. A unilateral resistance-training session consisting of knee extensor contractions will be completed. Following exercise cessation participants will ingest 0.33 g/kg body mass of either: a nonessential amino acid formulation or a plant-protein isolate. A saliva sample and a 2nd set of bilateral m.vastus lateralis microbiopsies are collected 3 h postingestion.

Official Title
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The Effect of a Plant Protein Isolate on Muscle Protein Synthesis in Humans at Rest, and After Resistance Exercise: A Randomized Controlled Trial

Conditions
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Metabolism, Exercise, Dietary Supplements

Intervention / Treatment
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* Dietary Supplement: NEAA
* Dietary Supplement: PPI


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: - Men and women aged 18 to 35 years Exclusion Criteria: Known intolerance to any item contained in the protein products. Blood borne disease risk, current illness or medication that would adversely affect participation Known adverse reaction to venepuncture and/or biopsy Inability to undertake resistance exercise Inability to adhere to protocol guidelines

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: Block randomized control trial, parallel group design
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Sham Comparator: Nonessential amino acid formulation (NEAA)<br>Novel non-essential amino acid blend dosed at 0.33 g/kg body mass in 400 ml of water. Postprandial muscle protein synthesis at rest, and after resistance exercise to be measured by deuterium incorporation in to skeletal muscle sampled by bilateral microbiopsies. Product Number: NEAA56812 | Dietary Supplement: NEAA<br>* Food grade mixture of non-essential amino acids in powdered form to be dissolved in water. Supplied by Marigot Ltd, Cork, Ireland<br>|
| Active Comparator: Plant Protein Isolate (PPI)<br>Plant protein isolate (Fava bean) dosed at 0.33 g/kg body mass in 400 ml of water. Postprandial muscle protein synthesis at rest, and after resistance exercise to be measured by deuterium incorporation in to skeletal muscle sampled by bilateral microbiopsies. Product Number: FP2011273 | Dietary Supplement: PPI<br>* Food grade plant (legume - Fava) protein isolate to be dissolved in water. Supplied by Marigot Ltd, Cork, Ireland<br>* Other names: Plant Protein Isolate;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Myofibrillar fractional synthetic rate change | The fractional rate of myofibrillar protein synthesis measured by deuterium incorporation into skeletal muscle sampled by microbiopsy | 4 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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amino acids, deuterium oxide, humans, protein synthesis, plant proteins, resistance training, skeletal muscle