Involvement of Plasmatic Factors in the Peripheral Muscle Dysfunction of COPD Patients - In Vitro Study

Study Overview
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Brief Summary
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Chronic Obstructive Pulmonary Patients is a defined by persistent airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways and the lung to noxious particles or gases. It is associated with an atrophy of the skeletal muscle that impairs the patient's prognosis. The biological mechanisms of this muscle atrophy have not been elucidated, and the spill-over theory has recently emerged. Indeed, in COPD patients, the pulmonary inflammation is associated with a systemic low-grade inflammation, and the increased pro-inflamatory molelcules in the blood - which constitute the cellular micro-environement of the muscle fibre - could activate the mechanisms of the cell atrophy.

Official Title
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Involvement of Plasmatic Factors in the Peripheral Muscle Dysfunction of COPD Patients - In Vitro Study

Conditions
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Chronic Obstructive Pulmonary

Intervention / Treatment
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* Other: Determination of pro-inflammatory plasma factors


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 35 to 85 years old COPD patients: association of symtoms (breathlessness, cough, sputum) and chronic exposure to inhalated risk factors (like tobacco smoke) and a FEV1/VC ratio <70% assessed by a spirometry Healthy subjects: A sedentariness assessed by a Voorrips score <9.4 and/or a report of <150 min/week of moderate-to-vigorous physical activity Exclusion Criteria: A recent COPD exacerbation (< 4weeks) The decompensation of a comorbidity An antioxidant supplementation or medication A long-term systemic corticosteroid medication

Ages Eligible for Study
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Minimum Age: 35 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy subjects<br>Determination of pro-inflammatory plasma factors at Healthy subjects aged from 35 to 85 years old | Other: Determination of pro-inflammatory plasma factors<br>* Determination of pro-inflammatory plasma factors at COPD patients aged from 35 to 85 years old and Healthy subjects aged from 35 to 85 years old<br>|
| COPD patients<br>Determination of pro-inflammatory plasma factors at COPD patients aged from 35 to 85 years old | Other: Determination of pro-inflammatory plasma factors<br>* Determination of pro-inflammatory plasma factors at COPD patients aged from 35 to 85 years old and Healthy subjects aged from 35 to 85 years old<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Myotube diameter of in vitro cultured myotubes | Myotube diameter of in vitro cultured myotubes from healthy subjects, exposed to the serum of healthy controls and COPD patients | 6 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Expression and translation of markers of | Expression and translation of markers of anabolic and catabolic pathways | 6 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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COPD (Chronic Obstructive Pulmonary Patients), Atrophy, cell cultur, systemic inflammation, Catabolism