Metabolic Availability of Lysine From Rice

Study Overview
=================
Brief Summary
-----------------
The purpose of this study is to investigate the digestibility of the amino acid lysine in cooked rice in adult men using stable isotope technique.

Detailed Description
-----------------
The investigators objective is to determine the metabolic availability (MA) of lysine in cooked white rice protein using the indicator amino acid oxidation (IAAO) technique. Five men will receive graded levels (20, 40, and 60, and 70%) of the lysine requirement of 35 mg/kg/d as a crystalline AA mixture,and cooked rice protein (20, 40 and 60%), respectively.

Official Title
-----------------
Application of the Indicator Amino Acid Oxidation Technique for the Determination of Metabolic Availability of Lysine From Cooked Rice Protein in Adult Men

Conditions
-----------------
Healthy Adults

Intervention / Treatment
-----------------
* Dietary Supplement: Amino Acid Crystalline Lysine Amino Acid Mix
* Dietary Supplement: Cooked Rice containing Lysine


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Healthy Adult males (18 to 50years old) Exclusion Criteria: A history of recent weight loss or illness Use of any medication at the time of entry into the study.

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
-----------------
Male

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Health Services Research
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Lysine Amino Acid<br> | Dietary Supplement: Amino Acid Crystalline Lysine Amino Acid Mix<br>* crystalline lysine from AA MIX (20, 40, and 60% of the mean lysine requirement of 35 mg/kg/d) Lysine will be given in the form of L-Lysine-HCl, Sigma-Aldrich Brand.<br>Dietary Supplement: Cooked Rice containing Lysine<br>* Lysine (20,40 and 60 % of the mean lysine requirement) from Cooked Rice<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | Baseline | 
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 15 min after intake of the fourth hourly meal (before tracer infusion)) | 
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 30 min after intake of the fourth hourly meal (before tracer infusion)) | 
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 45 min after intake of the fourth hourly meal (before tracer infusion)) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Metabolic Availability of Lysine | 4 breath samples will be taken to provide F13CO2 measurement (Level of phenylalanine oxidation).Breath samples will be collected and analyzed for 13CO2 enrichment. | Post Tracer Infusion (between 150 and 240 min of tracer infusion) | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
lysine, rice, bioavailability