Evaluation of the Ketogenic Potential of Different Diet Supplements

Study Overview
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Brief Summary
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Evaluate the capacity of different dietary supplements to increase ketone production in healthy adults.

Detailed Description
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The aim of this study is to evaluate the capacity of different dietary supplements to increase ketone production in healthy adults. Each supplement (leucine, butyrate, octanoate, carnitine or butter fraction rich in MCT) is evaluated for a period of 4-hour during which multiple blood samples are taken in order to measure ketone production.

Official Title
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Stimulating the Ketogenesis by Using Nutritional Supplements

Conditions
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Healthy Adults

Intervention / Treatment
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* Dietary Supplement: Control
* Dietary Supplement: Leucine
* Dietary Supplement: Butyrate -
* Dietary Supplement: Butyrate +
* Dietary Supplement: Octanoate -
* Dietary Supplement: Octanoate +
* Dietary Supplement: Carnitine
* Dietary Supplement: Butter fraction


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy adults aged of 18 years old or older. Non-smoking. Exclusion Criteria: Diabetes or prediabetes Uncontrolled hypertension Uncontrolled thyroid function Taking medication that will affect lipid/glucose metabolism Severe infection or inflammation Pregnancy

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Ketogenic potential<br>Each participant has to go through a total of 8 visits, each visit corresponding to a standardize breakfast taken alone (control visit) or with one of the dietary supplements evaluated followed by a period of 4-hour with multiple blood sampling. Intervention 1: Control; no supplement Intervention 2: 5 g of leucine Intervention 3: 3.6 g of butyrate Intervention 4: 7.2 g of butyrate Intervention 5: 5 g of octanoate Intervention 6: 10 g of octanoate Intervention 7: 1.95 g of carnitine Intervention 8: 65 g of butter fraction rich in MCT | Dietary Supplement: Control<br>* Visit corresponding to a standardize breakfast taken alone (control visit) followed by a period of 4-hour with multiple blood sampling.<br>* Other names: CTL;Dietary Supplement: Leucine<br>* Visit corresponding to a standardize breakfast with 5 g of leucine followed by a period of 4-hour with multiple blood sampling.<br>Dietary Supplement: Butyrate -<br>* Visit corresponding to a standardize breakfast with 3.6 g of butyrate followed by a period of 4-hour with multiple blood sampling.<br>Dietary Supplement: Butyrate +<br>* Visit corresponding to a standardize breakfast with 7.2 g of butyrate followed by a period of 4-hour with multiple blood sampling.<br>Dietary Supplement: Octanoate -<br>* Visit corresponding to a standardize breakfast with 5 g of octanoate followed by a period of 4-hour with multiple blood sampling.<br>Dietary Supplement: Octanoate +<br>* Visit corresponding to a standardize breakfast with 10 g of octanoate followed by a period of 4-hour with multiple blood sampling.<br>Dietary Supplement: Carnitine<br>* Visit corresponding to a standardize breakfast with 1.95 g of carnitine followed by a period of 4-hour with multiple blood sampling.<br>Dietary Supplement: Butter fraction<br>* Visit corresponding to a standardize breakfast with 65 g of butter fraction followed by a period of 4-hour with multiple blood sampling.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Concentration of Total Plasma Ketones | Difference from Time 0 in Total ketones = acetoacetate (umol/L) + beta-hydroxybutyrate (umol/L) Time 0 is when the participants arrived, after a 12 hours fast, i.e. before the breakfast. Plasma was collected every 30 minutes for 4 hours immediately following breakfast (where supplements were taken). Results are the mean of plasma ketones measured every 30 minutes ( i.e. total of 9 plasma samples) for the 4 hour period. For each time point, the difference form time 0 has been made. So this is the mean of the difference from time 0 in total ketones. Data were not collected for the 'Intervention 8: 65 g of butter fraction rich in MCT' supplement. | 4 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Ketones, Butyrate, Leucine, Octanoate, Carnitine, Medium Chain Triglycerides