Effect of Diet on the Microbiota / Endoccanabinoidome Axis in Response to Physical Activity

Study Overview
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Brief Summary
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Both the endocannabinoid system and the microbiome are highly conditioned by nutrition and physical activity, and have an interdependent, bidirectional relationship. We suggest studying the interleaving between the endocannabinoidome-microbiome axis and host metabolism under the combined effect of a diet and physical activity. More specificly, we will study the link between the impact of the diet on the intestinal microbiome and the endocannabinoid reaction after intense exercise.

Detailed Description
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Aims of the study are to compare the impacts of two short 7-day contrasted nutritional interventions (the Mediterranean diet (MED) and the Canadian diet (CAN)) on blood metabolites (endocannabinoids) after a maximal aerobic test. A 3 weeks washout between the two diets is planned. Gut microbiome at each test will also be evaluated.

Official Title
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Effect of Dietary Composition on the Gut Microbiota / Endocannabinoidome Axis in Response to Maximal Aerobic Exercise

Conditions
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Diet Habit, Microbial Colonization, Activity, Motor

Intervention / Treatment
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* Other: 3 maximal aerobic tests


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: women 20-30 years old healthy jogging >1h/week and >3h/week of physical activity bmi 18.5-25 Exclusion Criteria: postmenopausal, pregnancy or lactation pathology or medication smoking more than 7 alcohol consumption/week food allergy, food intolerance or food aversion important weight change in the last 6 months antibiotics in the last 3 months night worker pathologies

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Mediterannen diet<br>The MED diet was abundant in fiber, micronutrients and plant-based proteins. The diet was rich in essential FA like monounsaturated fatty acids (MUFA) and n-3. | Other: 3 maximal aerobic tests<br>* Maximal aerobic tests will be performed before diet intervention, after the first diet and after the second diet<br>|
| Experimental: Canadian diet<br>The NAM diet had a high content in saturated fatty acids (SFA) and simple sugar and was low in fiber. | Other: 3 maximal aerobic tests<br>* Maximal aerobic tests will be performed before diet intervention, after the first diet and after the second diet<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Endocannabinoid level post physical activity |  | 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Microbiome, Endocannabinoids