Effect of an Exercise Bout in Plasma and Fecal Profile

Study Overview
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Brief Summary
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The aim of this project is to study the effect of a bout of exercise on the metabolomic profile of plasma and feces as well as its influence on the intestinal microbiota.

Detailed Description
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The practice of physical exercise has numerous health benefits, preventing the appearance and development of cardiovascular diseases and various types of cancer. Exercise causes changes in the vascular, muscular and pulmonary systems, etc. One of the benefits that can result from exercise modification of the intestinal microbiota and its metabolic profile. In order to identify the potential changes that exercise has on the intestinal microbiota and its metabolic profile, the purpose of this study is to identify changes in the plasma and fecal metabolome and in fecal microbiota associated with a bout of exercise

Official Title
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Exploring the Effect of Exhaustive Exercise From a Metabolomic and Metagenomic Approach

Conditions
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Healthy

Intervention / Treatment
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* Other: Prolonged Exercise session


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18-45 years of age high physical condition (VO2 ≥ 55 ml/kg/min) BMI 18-25 kg/m2 Exclusion Criteria: antibiotics intake during 3 months prior to the study, smoking nutritional complements ergogenic complements prebiotics probiotics vegetarian or vegan diet chronic medication gastrointestinal surgery Any diagnosed disease

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Intervention group<br>Participants will exert an exercise prolonged session | Other: Prolonged Exercise session<br>* After a standardized warm-up of 10 minutes of continuous running on a treadmill at 60% of their maximum heart rate (HRmax), subjects will perform a maximum oxygen consumption test (VO2max) with a gas analyzer (UltimaTM Series, MGC Diagnostic Corporation, St. Paul, MN, USA. The protocol will start with a slope of 1% at a speed of 10 km/h, with increments of 0.3 km/h every 30 s until volitional exhaustion. Finally, ten minutes after the treadmill test, volunteers will run 1 kilometer (t1km) as fast as possible and the time needed to cover the distance will be recorded.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma metabolome profile | Abundance of blood metabolites, metabolic networks, and metabolic pathways activity | Changes from baseline to 30 min after the exercise intervention | 
| Fecal microbiota | Abundance of gut microbial taxa, communities, and metabolic pathways activity | Changes from baseline to 30 min after the exercise intervention |