Cardiovascular Effects of Incremental Diesel Exhaust Inhalation in Middle-Aged Healthy GSTM1 Null Human Volunteers

Study Overview
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Brief Summary
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Purpose: A growing body of epidemiological data suggests an increased risk of cardiovascular events associated with air pollutants. Reactive oxygen species (ROS) have been implicated as a potential mechanism for the adverse effects of air pollutants and genetic polymorphisms of the glutathione-s-transferases (GSTs) have been shown to participate in the antioxidant defenses to air pollutants. This study examined the dose effects of diesel exhaust exposure on the cardiovascular system in healthy middle-aged subjects. Participants: Six healthy 50-75 year-old male and female subjects with GSTM1 null genotype had 3 sequential exposures to the diesel exhausts at concentrations approximately 100 µg/m3, 200 µg/m3, and 300 µg/m3 for 2 hours with a about 2 weeks of interval between exposures.

Conditions
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Healthy

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 50-75 years old generally healthy male and female. Normal resting ECG. Oxygen saturation greater than 94% at the time of physical exam. Exclusion Criteria: A history of angina, cardiac arrhythmias, and ischemic myocardial infarction or coronary bypass surgery. Cardiac pacemaker. Uncontrolled hypertension (> 150 systolic, > 90 diastolic). Neurodegenerative diseases such as Parkinson's and Alzheimer disease. A history of chronic illnesses such as diabetes, cancer, rheumatologic diseases, immunodeficiency state, known cardiovascular disease, chronic respiratory diseases such as chronic obstructive pulmonary disease or severe asthma. History of bleeding diathesis. Currently taking HMG-CoA reductase inhibitors for hyperlipidemia including lovastatin, pravastatin, simvastatin, and atorvastatin. Currently taking beta-blockers to control hypertension and/or arrhythmias. Use of oral anticoagulants. Participants must refrain from all over-the-counter NSAIDs for a period of two weeks prior to exposure. Low-dose aspirin will be acceptable. Medications not specifically mentioned here may be reviewed by the investigators prior to a participant's inclusion in the study. Subjects who are currently smoking or have smoking history within 1 year of study (defined as more than one pack of cigarettes in the past year). Subject is pregnant, attempting to become pregnant or breastfeeding. No exposure will be conducted within 4 weeks of a respiratory tract infection.

Ages Eligible for Study
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Minimum Age: 50 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy middle-aged human volunteers<br> | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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risk of cardiovascular events