Effect of 4 Weeks Fish Oil Supplementation on Cycling Performance in Healthy Male Cyclists

Study Overview
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Brief Summary
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Research Plan Eleven trained cyclists have completed 4 cycling performance trials, before and after 4 weeks of fish oil supplementation and before and after 4 weeks of placebo with a 4 week washout between supplementation periods. During the performance trials blood, expired air and heart rate were collected. The blood samples have been used to investigate the production of inflammation resulting from the cycling performance test. Inflammation has been assessed using enzyme-linked immunosorbent assay for IL-6, which is an inflammatory cytokine. Work done from the cycling performance will be analysed to investigate whether there is a relationship with power and inflammation and whether this is modulated by fish oil supplementation. Specific Aims Does 4 weeks of Fish Oil supplementation attenuate the inflammatory response following high intensity cycling. Is a modulation of the inflammatory response connected to cycling performance. Hypotheses Four weeks of n3-PUFA supplementation will reduce the inflammatory response A reduction in inflammation will improve cycling performance Anticipated Outcomes A high level of cycling performance, indicated by a high average power, will be associated with high levels of systemic inflammation. n3-PUFA supplementation will decrease the level of inflammation and consequently cycling performance will be improved.

Official Title
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Effect of 4 Weeks Fish Oil Supplementation on Cycling Performance or Oxidative and Inflammatory Parameters in Healthy Male Cyclists

Conditions
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Exercise-induced Inflammation

Intervention / Treatment
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* Dietary Supplement: n-3 PUFA
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: recreationally trained male cyclists Exclusion Criteria: History of coagulation/bleeding disorder History of metabolic disease Serious allergy History of heart disease Regular use of anti-inflammatory drugs Vitamin or fish oil supplementation over the past 6 months Known to have blood-borne virus Females

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Randomised, placebo-controlled, double blind, crossover design study
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: n-3 PUFA<br>Omega-3 polyunsaturated fatty acid - 5.7g/ day for 4 weeks | Dietary Supplement: n-3 PUFA<br>* n-3 PUFA capsules containing EPA and DHA<br>* Other names: Omega-3;|
| Placebo Comparator: Placebo<br>Olive Oil - 6g/ day for 4 weeks | Dietary Supplement: Placebo<br>* Olive oil capsules<br>* Other names: Olive Oil;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Work Done | Work done measured in Joules from the Lode cycle ergometer during 15 minute cycling time trial | 15 minutes during time trial | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Exercise, Fish Oil, Cycling, Inflammation, Oxidative stress