Effect of Surgical Therapy on RANKL/OPG (Osteoprotegerin) System

Study Overview
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Brief Summary
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In the present clinical study including chronic, aggressive periodontitis and healthy individuals, the investigators aimed to determine the RANKL, osteoprotegrin (OPG), Interleukin-1 (IL-1β), IL-10, IL-1β levels in gingival crevicular fluid(GCF) before and after surgical periodontal treatment and search out the relationship of clinical parameters with these cytokine levels.

Official Title
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Effect of Surgical Periodontal Therapy on the RANKL/OPG System : Do we Need Osteo-immune Modulation Targeting RANKL?

Conditions
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Chronic Periodontitis, Aggressive Periodontitis, RANKL/OPG Ratio, Gingival Crevicular Fluid

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Chronic periodontitis having at least 5mm or more probing depth and aggressive periodontitis patients. Exclusion Criteria: Any known systemic disorder, used antibiotics and/or anti-inflammatory medications, had periodontal treatment in the last 3 months and all of them were non smokers. Subjects who were lactating and pregnant were also excluded.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Chronic periodontitis<br>Surgical periodontal treatment and GCF collection 3 months after treatment | |
| Agressive periodontitis<br>Surgical periodontal treatment and GCF collection 3 months after treatment. | |
| Control group<br>Periodontally healthy individuals | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| RANKL (pmol) level in GCF | Changes in RANKL levels 3 months after surgical periodontal treatment in Chronic and Aggressive periodontitis determined by ELISA | Baseline-3 months | 
| OPG level (pg) in GCF | Changes in OPG levels 3 months after surgical periodontal treatment in Chronic and Aggressive periodontitis determined by ELISA | Baseline-3 months | 
| RANKL/OPG ratio | RANKL/OPG ratio changes 3 months after surgical periodontal treatment in Chronic and Aggressive periodontitis | Baseline - 3months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| IL-1β (pg) levels in GCF | Changes in IL-1β levels (determined by ELISA) 3 months after surgical periodontal treatment in Chronic and Aggressive Periodontitis groups | Baseline, 3 months after treatment | 
| Il-10 (pg) levels in GCF | Changes in IL-10 levels (determined by ELISA) 3 months after surgical periodontal treatment in Chronic and Aggressive periodontitis | Baseline-3 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Chronic Periodontitis, Aggressive Periodontitis, RANKL/OPG ratio, Gingival crevicular fluid