Evaluation of Skeletal Muscle Lipid Content by Magnetic Resonance Spectroscopy

Study Overview
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Brief Summary
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Type 2 diabetes is a growing public health issue and one component of Type 2 diabetes is 'insulin resistance - the ability of insulin to work less well. The skeletal muscle is one of the largest users of sugar in the body, when the skeletal muscle is insulin resistant, this contributes to diabetes. There is some evidence that the amount of fat in the skeletal muscle is related to the amount of insulin resistance. We are asking people to participate in a study to look at the amount of fat in muscle and how this fat amount changes with exercise. We hypothesize that heavier people will have higher amounts of fat and deplete this fat more slowly than lean subjects.

Detailed Description
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High intramyocellular lipid (IMCL) content in skeletal muscle has been correlated to insulin resistance in obese subjects. Yet, endurance athletes have high intramyocellular lipid content and very low insulin resistance. There are several explanations for this paradox. This study will use a new magnetic resonance imaging technique to look at the amount of fat in the muscle and its depletion with exercise. We hypothesize that overweight subjects will have higher amounts of fat and deplete this fat more slowly than lean subjects.

Official Title
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Evaluation of Intramyocellular Lipid Content (IMCL) in Skeletal Muscle by Magnetic Resonance Spectroscopy

Conditions
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Healthy

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age range will be from age 18 to 45. We will be limiting the upper age range because increased age is associated with sarcopenia and alteration of fiber type. We will define the lean trained group by a BMI of < 24 kg/m2. We will define the overweight group as BMI > 26 25 kg/m2. The subjects should be participating in regular running exercise (> 30 min/day, ≥ 5 days/week). Weight must be stable [+/- 5 pounds] for at least the three months prior to the study for all participants. Exclusion Criteria: The subjects must not be on medications that may affect lipid levels, specifically lipid lowering agents, birth control pills, or diuretics. The subjects should not be on a high fat diet (> 45% fat) as measured by a screening questionnaire. The subject must not have any contraindications to magnetic resonance imaging.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| 1<br>overweight runners (BMI>25) | |
| 2<br>lean runners (BMI<24) | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| absolute change in intramyocellular lipid quantity with exercise |  | over 4 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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training, intramyocellular lipid, insulin resistance