Investigate the Role of microRNA in Chronic Rhinosinusitis From DC-Th Axis

Study Overview
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Brief Summary
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This study aimed to evaluate the upstream events of Th17/Treg imbalance in CRS and their immune regulatory factors. Then the investigators aimed to explore the regulatory role of miRNA on DC-Th axis and its dysfunction in the pathogenesis of CRS, in order to determine the miRNA expression profile in CRS and clarify the role of miRNA in the pathogenesis of CRS by regulating the DC-Th axis.

Detailed Description
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Chronic rhinosinusitis (CRS), commonly encountered in the ﬁeld of clinical otorhinolaryngology, is still a challenging proposition for doctors because of its high incidence and the unsatisfactory treatment outcomes. Nowadays, studies on the pathogenesis of NP are still attached great importance by researchers from each country. NP has become a global health problem with a considerable socioeconomic burden. Recently, research showed that the impaired balance of Th17/Treg was the significant basis of NP. Nevertheless, the pathogenesis of Th17/Treg imbalance was unclear. In this study, DC-Th axis was designed as the main line, and the regulation of miRNA on DC was designed as the entry point. This study aimed to evaluate the upstream events of Th17/Treg imbalance in CRS and their immune regulatory factors. Then the investigators aimed to explore the regulatory role of miRNA on DC-Th axis and its dysfunction in the pathogenesis of CRS, in order to determine the miRNA expression profile in CRS and clarify the role of miRNA in the pathogenesis of CRS by regulating the DC-Th axis. This study will play an important role in clarifying the pathogenesis of CRS eventually and will fill the blanks of the research between miRNA and CRS in the investigators'country. This study is with important clinical value for establishing control strategies for CRS.

Official Title
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Investigate the Role of microRNA in Chronic Rhinosinusitis From DC-Th Axis

Conditions
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Rhinosinusitis

Intervention / Treatment
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* Genetic: miRNA mimics or inhibitors


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: CRS diagnosis is accord with diagnostic criteria of The European nasal sinusitis, nasal polyps guidelines (EPOS 2012) . Selected subjects are stopped using systemic or topical corticosteroids in one month before experiment. All the patients have sinus CT and nasal endoscopy and allergen Skin Prick Test in preoperative. Aged from 18 to 75 years old. Woman or man. Exclusion Criteria: Choanal polyp, fungal nasal sinusitis, cystic fibrosis. Acute upper respiratory tract infection and other diseases associated with the body.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| control<br>Patients with only nasal septum deviation | Genetic: miRNA mimics or inhibitors<br>* DCs are transfected by the miRNA mimics or inhibitors after they are separated from peripheral blood mononuclear cells in CRS patients<br>|
| CRS sNP<br>Chronic Rhinosinusitis patients without nasal polyps | Genetic: miRNA mimics or inhibitors<br>* DCs are transfected by the miRNA mimics or inhibitors after they are separated from peripheral blood mononuclear cells in CRS patients<br>|
| atopic CRS wNP<br>Chronic Rhinosinusitis patients with allergic constitution and nasal polyps | Genetic: miRNA mimics or inhibitors<br>* DCs are transfected by the miRNA mimics or inhibitors after they are separated from peripheral blood mononuclear cells in CRS patients<br>|
| non-atopic CRS wNP<br>Chronic Rhinosinusitis patients with nasal polyps but not allergic constitution | Genetic: miRNA mimics or inhibitors<br>* DCs are transfected by the miRNA mimics or inhibitors after they are separated from peripheral blood mononuclear cells in CRS patients<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Abnormal microRNA in the Chronic Rhinosinusitis are found by gene chip and qPCR. |  | 6 to 24 months post procedure | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Chronic rhinosinusitis, microRNA, dendritic cell, helper T cell