Oxidative Stress on Muscle Dysfunction in Hemodialysis Patient

Study Overview
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Brief Summary
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In hemodialysis patient (HD) a reduction of the skeletal muscle mass and strength has previously been reported. This muscle impairment constitutes an independent prognosis factor in HD patients. Oxidative stress and inflammation have been linked to the muscle impairment. The mitochondria is a classical producer and target of reactive oxygen species (ROS), and may thus constitute a central actor of the skeletal muscle impairment in HD patients. Therefore, the aim of the present study is to investigate the role of the muscle mitochondrial density on the muscle impairment in HD patients, in comparing the muscle mitochondrial density and oxidative stress in HD patients vs. healthy matched controls. In order to assess the effect of the oxidative stress and inflammation on the muscle impairment in HD patients, muscle function assessements will be performed after renal transplantation (which lowers the oxidative stress and inflammation levels) in the HD patient group. No therapeutic intervention will be tested in the present study.

Official Title
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Impact of the Oxidative Stress on the Skeletal Muscle Dysfunction in Hemodialysis Patient

Conditions
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Chronic Renal Failure Requiring Hemodialysis

Intervention / Treatment
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* Procedure: renal transplantation
* Other: No intervention


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age between 40-80 years old less than 150min by week of moderate to vigorous physical activity Chronic renal failure (glomerular filtration rate less than 15mL by min) requiring hemodialysis Patient placed on the kidney transplant waiting list of MontpellierCHU No indication against temporary kidney transplant Review cardiovascular older than two years Patient not under guardianship or trusteeship Clinically stable state Absence or recent infection within 3 months prior to inclusion Absence of progressive neoplasia No recent acute decompensation of chronic disease (thrust of heart failure, respiratory decompensated cirrhosis). Absence of recent stroke musculoskeletal and neurological states of the lower limbs which allow the realization of the proposed functional tests. Patient has given written informed consent Absence of congenital or genetic degenerative muscle disease Exclusion Criteria: Terminal chronic renal failure without replacement therapy Local anesthesic hypersensibility Corticosteroid treatment upper than 3 month COPD Oral anticoagulants Previous renal transplantation Subject not affiliated with a social security scheme, beneficiary or not such a plan Major subject protected by law or unable to consent under Article L 1121-8 of the Public Health Code Vulnerable person under Article L.1121-6 of the Public Health Code

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: HD patient<br> | Procedure: renal transplantation<br> <br> |
| Other: Healthy volunteers<br> | Other: No intervention<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle mitochondrial density assessed on a quadriceps biopsy by immunochemistry |  | 2 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Maximum voluntary contraction of the quadriceps (in Newton.meter) |  | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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hemodialysis, muscle dysfunction, mitochondria, oxidative stress, inflammation, renal transplantation