The Diversity of the Fungal and Bacterial in Colon Mucosa of Patients With Different Degree of Inflammation of Ulcerative Colitis

Study Overview
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Brief Summary
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We analyzed the diversity of the fungal and bacterial within colon mucosa between patients with different degree of inflammation of Ulcerative Colitis.

Detailed Description
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In recent years, the relationship of intestinal fungus with inflammatory bowel disease was gradually revealed. We divided the patients with ulcerative colitis into three groups on the basis of World Gastroenterology Organisation (WGO) classification about inflammation degree and progress classification and established the healthy control group. Analyzing the diversity of the fungal and bacterial within colon mucosa between patients with different clinical classification, for example, the proportion of bacteria and fungi, the proportion of candida albicans, the proportion of yeasts, and so on.

Official Title
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The Diversity of the Fungal and Bacterial in Colon Mucosa of Patients With Ulcerative Colitis Associated Different Clinical Classification

Conditions
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Ulcerative Colitis

Intervention / Treatment
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* Other: ulcerative colitis
* Other: Control


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients at our tertiary care medical center, aged between 18 and 80 years, scheduled to undergo surveillance colonoscopy. The patients with ulcerative colitis for ulcerative colitis group. The patients without ulcerative colitis for control group. 18-80 years. Exclusion Criteria: Using antibiotics, glucocorticoid and immunosuppressants wintin one month. There are contraindications for enteroscopy and biopsy. There are other intestinal diseases. Pregnancy or breast-feeding women. Patients with serious lung disease and mental illness.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| ulcerative colitis<br>We analysed the diversity of the fungal and bacterial in patients with Ulcerative Colitis (UC). We take 2 pieces of biopsies in the sigmoid colon mucosa inflammation area during the colonoscopy and then perform gene sequencing. | Other: ulcerative colitis<br>* We take 2 pieces of biopsies in the sigmoid colon mucosa inflammation area during the colonoscopy and then perform gene sequencing.<br>* Other names: Patients With ulcerative colitis;|
| Control<br>Analysing the diversity of the fungal and bacterial in patients without ulcerative colitis. We take 2 pieces of biopsies in the sigmoid colon mucosa during the colonoscopy and then perform gene sequencing. | Other: Control<br>* We take 2 pieces of biopsies in the sigmoid colon mucosa during the colonoscopy and then perform gene sequencing.<br>* Other names: Patients Without ulcerative colitis;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| fungus detection rate | Fungi detection rate was defined as the percentage of patients in whose colonic mucosa at least one kind of fungus were identified. | 6 months | 
| yeasts detection rate | Yeasts detection rate was defined as the percentage of patients in whose colonic mucosa yeasts were identified. | 6 months | 
| candida albicans detection rate | Candida albicans detection rate was defined as the percentage of patients in whose colonic mucosa candida albicans were identified. | 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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fungal, mucosa, diversity, Basidiomycete