Discovery of New Early Detection Biomarkers of Acute Respiratory Distress Syndrome

Study Overview
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Brief Summary
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The goal of this project is to find a series novel biomarkers by differential proteomic techniques that can improve the early diagnosis and develop a more efficient therapy to enhance ARDS patient survival rate.

Detailed Description
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Acute respiratory distress syndrome(ARDS) has a very poor prognosis and high mortality. To improve the early diagnosis of ARDS, there is an urgent need for novel biomarkers of ARDS. The goal of this project is to find a series novel biomarkers by differential proteomic techniques that can improve the early diagnosis and develop a more efficient therapy to enhance ARDS patient survival rate. Clinical data and blood sample were recorded before treatment and after treatment. Acute Physiology And Chronic Health Evaluation III (APACHE III) scores were calculated at enrolment. Studying blood specimens from different period ARDS patients by proteomic analysis. It can provide information on a complex composition of proteins that are differentially expressed, which could be used for discovering high sensitivity and specificity ARDS biomarker.

Official Title
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Discovery of New Early Detection Biomarkers of Acute Respiratory Distress Syndrome

Conditions
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Acute Respiratory Distress Syndrome

Intervention / Treatment
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* Other: Blood samples collection before treatment
* Other: Blood samples collection after treatment
* Other: laboratory biomarker proteomic analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The Berlin definition of acute respiratory distress syndrome ATS definition of severe pneumonia Exclusion Criteria: age below 18 years pregnancy Expected survival under 24 hours

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| 1: patient at High risk for ARDSp<br>Severe pneumonia and（PaO2/FIO2）>300mmHg | Other: Blood samples collection before treatment<br> <br> Other: Blood samples collection after treatment<br> <br> Other: laboratory biomarker proteomic analysis<br> <br> |
| 2: patient at High risk for ARDSexp<br>Severe sepsis and without ARDS | Other: Blood samples collection before treatment<br> <br> Other: Blood samples collection after treatment<br> <br> Other: laboratory biomarker proteomic analysis<br> <br> |
| 3: mild ARDS<br>PaO2/FiO2=201～300 mmHg，and PEEP or CPAP≤5 cm | Other: Blood samples collection before treatment<br> <br> Other: Blood samples collection after treatment<br> <br> Other: laboratory biomarker proteomic analysis<br> <br> |
| 4: moderate ARDS<br>PaO2/FIO2=101～200 mmHg，且PEEP≥5 cm H2O | Other: Blood samples collection before treatment<br> <br> Other: Blood samples collection after treatment<br> <br> Other: laboratory biomarker proteomic analysis<br> <br> |
| 5: severe ARDS<br>PaO2/FIO2≤100 mmHg，且PEEP≥10 cm H2O | Other: Blood samples collection before treatment<br> <br> Other: Blood samples collection after treatment<br> <br> Other: laboratory biomarker proteomic analysis<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Analysis of serum proteins directly by proteomics analysis to identify new biomarkers of ARDS |  | baseline | 
| Analysis of serum proteins directly by proteomics analysis to identify new biomarkers of ARDS |  | 1 month | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| APACHE III score |  | baseline, 1week, 2 weeks | 
| PaO2/FiO2 ratio |  | baseline, day 3, day 5, 1week, 2 weeks | 
| Mortality or multi-organ failure |  | 1 month | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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ARDS, biomarkers, proteomic