The Relationship Between Arsenic Methylation Capability and Blood Metals in Children With Developmental Delays

Study Overview
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Brief Summary
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The purpose of this study is to explore the relationship between arsenic methylation and blood metals in children with developmental delays and its correlation with health related quality of life.

Detailed Description
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The investigators recruited children with developmental delays and children with normal development from Shin-Kong Ho-Su Memorial Hospital as controls. Information obtained from the interview included socioeconomic and basic demographic characteristics as well as children function, health condition, and quality of life. On the other hand, blood and urine samples of the participants were collected for the analysis of arsenic methylation related enzymes using polymerase chain reaction and restriction fragment length polymorphism. 2 ml blood were digested for the determination of mercury, lead and arsenic. The relationship between arsenic methylation and blood metals in children with developmental delays and its correlation with health related quality of were analyzed.

Official Title
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The Relationship Between Genetic Polymorphism of Arsenic Methylation Enzymes, Arsenic Methylation Capability and Blood Metals, and Developmental Delays in Preschool Children

Conditions
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Developmental Delays

Intervention / Treatment
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* Other: study group


Participation Criteria
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Eligibility Criteria
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Study group: Inclusion Criteria: children with developmental delays obtained written informed consent from parents Exclusion Criteria: parents refused for participation of their children to the intervention failed to obtain the written informed consent from parents Control group: Inclusion Criteria: children with normal development obtained written informed consent from parents Exclusion Criteria: parents refused for participation of their children to the intervention failed to obtain the written informed consent from parents

Ages Eligible for Study
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Minimum Age: 2 Years
Maximum Age: 12 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| study group<br>children confirmed to have developmental delays | Other: study group<br>* Explore the relationship of heavy metals and health related quality of life, including the relationship between the developmental delays and polymorphism of arsenic methylation enzymes, arsenic methylation capability and blood metals. Identify the interaction of children function, health condition, and quality of life and arsenic methylation and blood metals in children with developmental delays.<br>|
| control group<br>children with typical development | Other: study group<br>* Explore the relationship of heavy metals and health related quality of life, including the relationship between the developmental delays and polymorphism of arsenic methylation enzymes, arsenic methylation capability and blood metals. Identify the interaction of children function, health condition, and quality of life and arsenic methylation and blood metals in children with developmental delays.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| heavy metal assessment | laboratory analysis | 3 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| quality of life | questionnaires analysis | 2 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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arsenic, heavy metal