Multi-elemental Imaging of Lung Tissues With LIBS (Laser-induced Breakdown Spectroscopy)

Study Overview
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Brief Summary
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Evaluate the feasibility of performing a multi-elemental imaging analysis of lung specimens from patients with ILDs, with an technology named LIBS (Laser Induced-Breakdown Spectroscopy)

Detailed Description
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There is a major health problem due to the environmental and occupational related exposures to mineral particles, metals, and dust. The impact on health is considerable in terms of prevalence, morbidity and healthcare costs. Pathologists rarely report the presence of a possible mineral or metal-related etiology in a specimen by lack of available and convenient technology. Therefore, several respiratory diseases are considered idiopathic. Interstitial lung disease (ILD), also known as diffuse parenchymal lung disease (DPLD) is a group of lung diseases affecting the interstitium (the tissue and space around the air sacs of the lungs). In France, 15.000 new cases of ILD are diagnosed each year. ILD may be classified according to the cause. Idiopathic ILD are ILD of unknown causes. The most frequent idiopathic ILD are sarcoidosis and idiopathic pulmonary fibrosis (IPF).These two diseases are the core topic of the present study since they may relate to important mineral or metal exposure. The overall goal of this MEDICO-LIBS study is to evaluate the feasibility of using a laser-induced breakdown spectroscopy (LIBS)-based instrument to image(ie, identify, localize and possibly quantify) metals within selected human specimens with ILDs.

Official Title
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Multi-elemental Imaging of Lung Tissues With LIBS (Laser-induced Breakdown Spectroscopy) : a Feasibility Study

Conditions
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Interstitial Lung Disease, Sarcoidosis, Idiopathic Pulmonary Fibrosis

Intervention / Treatment
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* Diagnostic Test: Elemental analysis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient with ILD Patient informed, and agreed to participate Exclusion Criteria: Patient opposed to participating in the study. Patient without available specimen. Patient under legal protection

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Interstitial Lung Disease<br>Ex : sarcoidosis, IPF | Diagnostic Test: Elemental analysis<br>* LIBS multi elemental analysis of the specimen<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Generation of multi-elemental images in tissues with LIBS | Evaluation of the feasibility of collecting and describing LIBS multi-elemental images of lung specimens originating from patients with ILDs | Within a month after reception of the biopsy by the analytical laboratory | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Description of elemental distribution in the specimens | Comprehensive chemical characterization of both endogenous and exogenous elements for each specimen | Within a month after reception of the biopsy by the analytical laboratory | 
| Interpretation of the elemental maps | Understanding of the elemental distribution according to the clinical data | Within a month after reception of the biopsy by the analytical laboratory | 
| Comparison of the elemental distributions with results of other mineralogical investigations | Only for patients who previously benefited from other mineralogical investigations (eg, SEM-EDX), comparison of mineralogical analysis obtained with the elemental distributions obtained with LIBS | Within a month after reception of the biopsy by the analytical laboratory | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Laser Induced Breakdown Spectroscopy, Multi elemental imaging