Study of the Cutaneous Reinnervation After Burn

Study Overview
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Brief Summary
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The aim of this study is to understand why the regeneration of the cutaneous sensory nerve fibers is poor in post-burn scar. The presence or the lack of several molecular factors known to regulate neuronal cell development will be evaluate and compared within biopsies from healthy skin and post-burn scar.

Official Title
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Study of the Cutaneous Reinnervation After Burn With the Aim of a Better Care of Neuropathic Pain and Pruritus After Healing

Conditions
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Post-burn Innervation

Intervention / Treatment
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* Procedure: Skin biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Major patients At least one year back deep burn (2nd and 3rd degrees), and allowing the comparison between the post-burn scar dermatome and the healthy opposite dermatome Patients always followed in the service of Plastic surgery of the hospital of Brest Informed consent and paper of the patient after information of the objective of the study and the protocol Analog Visual scales for pain and/or pruritus (EVA): greater than or equal to 3 Simple Verbal scales for the pain and/or the pruritus ( EVS): pain / pruritus moderated to intense Membership to the Social Security Exclusion Criteria: Allergy to xylocaine Non-Caucasian patients Treatments having an effect on the pain and/or the pruritus Sun or UV exposure several days preceding or following the biopsies

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Skin biopsy<br>On the same patient, a biopsy of post-burn scar is removed. A second one is removed from healthy skin. | Procedure: Skin biopsy<br>* 2 biopsies are done on the same patient : 20 cutaneous biopsies from post-burn scar and from healthy skin<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of the differences of expression of genes coding for regulating proteins of the innervation | To evaluate the differences of expression of genes coding for regulating proteins of the innervation on samples of healthy skins and post-burn scar. These biopsies will be removed from post-burn patients (at least one year after burn) and allowing the comparison between conflicting dermatomes: a healthy dermatome and a formerly burned dermatome | 6 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Validation of the difference of density of intra-epidermic nerve fibers | To validate the existence of a difference of density of intra-epidermal nerve fibers between the healthy skins and the post-burn scar. | 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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molecular factors, cutaneous innervation, skin biopsy, post-burn scar