A Clinical Research of LeY-Targeted CAR-T in Myeloid Malignancies

Study Overview
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Brief Summary
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The overall purpose of this study is to explore the therapeutic effect of LeY-targeted chimeric antigen receptor T(CAR-T) cells in the treatment of myeloid malignancies.

Detailed Description
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Lewis -Y（LeY） is a difucosylated carbohydrate antigen and although its function is not known, it is expressed on a wide range of malignancies including myeloid malignancies. LeY-targeted CAR-T in myeloid malignancies had been explored and showed good efficiency in the treatment of myeloid malignancies. This trial is designed and conducted to test the safety and effectiveness of LeY-targeted CAR-T.

Official Title
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A Clinical Research of LeY-Targeted CAR-T in Myeloid Malignancies

Conditions
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Myeloid Malignancies

Intervention / Treatment
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* Biological: Anti-LeY-CAR-transduced T cells


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: LeY-expressing myeloid malignancy must be assured and must be relapsed or refractory disease. According to current traditional therapies, there must be no available alternative curative therapies and subjects must be either ineligible for allogeneic stem cell transplant (SCT), have refused SCT, or have disease activity that prohibits SCT at this time. Patients enrolled must have an evaluated score above 60 with KPS. LeY expression of the malignant cells must be detected by immunohistochemistry or by flow cytometry. Gender is not limited, age from 14 years to 75 years. Patients must have measurable or evaluable disease at the time of enrollment, which may include any evidence of disease including minimal residual disease detected by flow cytometry, cytogenetics, or polymerase chain reaction (PCR) analysis. Patients are expected to survive for more than 3 months by their physicians at the time of enrollment. Adequate absolute CD3 count estimated need to be assured for obtaining target cell dose based on dosage cohorts. Subjects with the following CNS status are eligible only in the absence of neurologic symptoms suggestive of CNS leukemia, such as cranial nerve palsy: CNS 1, defined as absence of blasts in cerebral spinal fluid (CSF) on cytospin preparation, regardless of the number of WBCs; CNS 2, defined as presence of < 5/uL WBCs in CSF and cytospin positive for blasts, or > 5/uL WBCs but negative by Steinherz/Bleyer algorithm CNS3 with marrow disease who has failed salvage systemic and intensive IT chemotherapy (and therefore not eligible for radiation) Ability to give informed consent. Cardiac function: Left ventricular ejection fraction greater than or equal to 40% by MUGA or cardiac MRI, or fractional shortening greater than or equal to 28% by ECHO or left ventricular ejection fraction greater than or equal to 50% by ECHO. Renal function: Creatinine level of peripheral blood is required no greater than 133umol/L. Females of child-bearing potential must have a negative pregnancy test because of the potentially dangerous effects on the fetus. Patients with history of allogeneic stem cell transplantation are eligible if there is no evidence of active GVHD and no longer taking immunosuppressive agents for at least 30 days prior to enrollment. Patients volunteer to participate in the research. Exclusion Criteria: Evident signs suggesting that patients are potentially allergic to cytokines. Frequent infection history and recent infection is uncontrolled. Patients with concomitant genetic syndrome: patients with Down syndrome, Fanconi anemia, Kostmann syndrome, Shwachman syndrome or any other known bone marrow failure syndrome Active acute or chronic graft-versus-host disease (GVHD) or requirement of immunosuppressant medications for GVHD within 4 weeks of enrollment. Concurrent use of systemic steroids or chronic use of immunosuppressant medications. Recent or current use of inhaled steroids is not exclusionary. For additional details regarding use of steroid and immunosuppressant medications. Pregnancy and nursing females. HIV infection. Active hepatitis B or active hepatitis C. Participation in a prior investigational study within 4 weeks prior to enrollment or longer if required by local regulation. Participation in non-therapeutic research studies is allowed. Class III/IV cardiovascular disability according to the New York Heart Association Classification. Patients with a known history or prior diagnosis of other serious immunologic, malignant or inflammatory disease. Other situations we think not eligible for participation in the research.

Ages Eligible for Study
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Minimum Age: 14 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Myeloid Malignancies<br>The trial will be conducted in a manner of simon two-stage design with Anti-LeY-CAR-transduced T cells, beginning in the first stage with the aim of over 30% reaction rate among 15 patients with myeloid malignancies. Only when the expected reaction rate is achieved the 30 patients left can be recruited. | Biological: Anti-LeY-CAR-transduced T cells<br>* The first 3 enrolled patients will receive autologous-derived LeY-targeted CAR-T cells on day 1, 2 and 3 with respective 10%, 30% and 60% of the total expected dosage after receiving lymphodepleting chemotherapy. If the 3 patients don't display severe toxicity,the next patients enrolled will get infused in 2 days with respective 40% and 60% total dosage.<br>* Other names: LeY-targeted CAR-T cells;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Adverse Events That Are Related to Treatment | Determine the toxicity profile of the LeY targeted CAR T cells with Common Toxicity Criteria for Adverse Effects (CTCAE) version 4.0. | 3 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| In vivo existence of Anti-LeY CAR-T cells |  | 3 years | 
| Reaction Rate of Treatment |  | 3 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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LeY, Myeloid Malignancies, CAR-T