Study About High Fat Meal and Postprandial Lipemia

Study Overview
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Brief Summary
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The hypothesis of the proposed project is that after a fat overload, the postprandial response is different in both groups, suggesting that the LPP will present the most significant damage in endothelial vasomotion in obese individuals, especially those with GI and T2DM. After the fat overload, we hypothesized that there will be a worsening of endothelial function and microvascular reactivity in OB/DM2 and OB group compared to C, but also find lower concentrations of incretins in OB/DM2 group compared to other groups. These hypotheses may be confirmed by techniques for evaluating microvascular function, the use of DFT skin for vasomotion evaluation and finally analysis of analytes through metabolic and cardiovascular read by Multiplex kit.

Detailed Description
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Will be recruited 60 women aged 19-40 years, with a diagnosis of obesity (BMI ≥ 30 kg/m2) treated at the obesity clinic at the State University of Rio de Janeiro (UERJ)at Brazil. Microcirculation parameters and blood tests will be assessed at baseline and after this, the women will receive a high-fat meal containing croissant, salami, whole milk and cheddar cheese. After 30, 60, 120 and 180 minutes this meal the microcirculation will be evaluated as well as blood collection.

Official Title
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Evaluating the Effect of Lipid Overload in Endothelial Function and Microvascular Reactivity in Young Obese Women

Conditions
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Obesity

Intervention / Treatment
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* Dietary Supplement: High fat meal


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: all patients should have obesity class I (BMI between 30 and 35kg/m2); Submit diabetes mellitus without further treatment or be obese non-diabetic or glucose intolerant; Presenting the age between 19 to 40 years. Waist circumference> 80 cm (IDF) Exclusion Criteria: Renal disease, coronary or peripheral vascular, hematologic or hepatic impairment; Presence of dyslipidemia; smokers; Significant loss of body weight six months prior to the study.

Ages Eligible for Study
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Minimum Age: 19 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Obese Group<br>Obese group with fat overload intake. | Dietary Supplement: High fat meal<br> <br> |
| Other: Control Group<br>Control Group + fat overload intake | Dietary Supplement: High fat meal<br> <br> |
| Other: Glucose Intolerance<br>glucose intolerance + fat overload intake | Dietary Supplement: High fat meal<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Microvascular function |  | up to 180 minutes after high fat meal | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| incretins hormones | We will assess incretins hormones by multiplex | basal, 30, 60, 120,180 minutes after high fat meal | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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glucose intolerance (20), Control (20)