Protective Effects of Exercise Training

Study Overview
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Brief Summary
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Rationale. Sleep loss is a risk factor for cardiovascular events mediated through endothelial dysfunction. Objective. To determine if 7 weeks of exercise training can limit cardiovascular dysfunction induced by total sleep deprivation (TSD) in healthy young men. Methods: 16 subjects will be examined during 40-h TSD, both before and after 7 weeks of interval exercise training. Vasodilatation induced by ACh, insulin and heat (42°C) as well as pulse wave velocity (PWV), blood pressure and heart rate (HR) will be assessed at baseline, during TSD, and after one night of sleep recovery. Biomarkers of endothelial activation, inflammation, and hormones will bemeasured from morning blood samples at 07:00

Official Title
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Protective Effects of Exercise Training on Endothelial Dysfunction Induced by Total Sleep Deprivation in Healthy Subjects

Conditions
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Sleep Deprivation

Intervention / Treatment
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* Other: Sleep deprivation


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy Men 18 - 35 yrs Exclusion Criteria: Consumming 400 mg of caffeine per day, Body mass index (BMI) greater than 30 kg/m², Taking medication. Excessive daytime somnolence (Epworth Sleepiness Scale >9) Sleep complaints (Pittsburgh Sleep Quality Index >5) Inability to be considered as an intermediate chronotype on the Horne and Ostberg questionnaire (score <31 or >69) Traines subject (Ricci Gagnon questionnaire >35)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Exercise training<br>7 weeks of exercise training | Other: Sleep deprivation<br>* 40 hours of continuous wakefullness<br>|
| Sham Comparator: Control<br>Sedentary group | Other: Sleep deprivation<br>* 40 hours of continuous wakefullness<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| ACh induced Vasodilation during TSD | Cutaneous vasodilation (in % Baseline) induced by local application of Acethylcholine | Up to 09:00 during sleep deprivation (i.e. after 26 hours of wakefulness) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Insuline Vasodilation | Cutaneous vasodilation (in % Baseline) induced by local application of insulin | Up to 09:00 during the sleep deprivation day and the recovery day | 
| Heat vasodilation | Cutaneous vasodilation (in % Baseline) induced by local application of heat (42°C) | Up to 9:00 during the sleep deprivation day and during the recovery day | 
| Inflammation | Plasma levels of inflammatory marquers (TNF-alpha, IL1beta...) | Up to 7:00 during the sleep deprivation day and during the recovery day. | 
| blood pressure | Mean, systolic and diastolic blood pressure | Every 3 hours during the 40 hours of wakefullness (i.e. total sleep deprivation) | 
| PWV | Pulse wave velovity evaluated by echography | Up to 09:00 during the sleep deprivation day | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Exercise training, Endothelial function