Molecular & Cellular Characterisation of Oral Lichen Planus

Study Overview
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Brief Summary
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The principal research objective is to provide enhanced understanding of the cellular and molecular events important in the pathogenesis of Oral Lichen Planus to enable improved diagnosis and development of novel treatments for patients.

Detailed Description
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The following questions will also be addressed: Are the histological architectural tissue changes that take place in oral lichen planus quantifiable using computer based imaging, graph theory and fractal geometry principles? Quantification of these changes would allow the development of a tool to facilitate the accurate measurement of response to treatment. What is the nature of such architectural changes and what are the differences with normal, dysplastic and neoplastic epithelia (the investigators have morphometrical data collected from previous research to allow such comparisons). Is it possible to produce evidence-based statistical classification into established diagnostic classes using the proposed methodology? This would contribute towards making histopathological diagnosis more quantitative, reproducible and accurate. Is it possible to automate such morphometrical analysis/classification? This would allow large data sets to be screened automatically in a shorter time frame and at lower cost than human based screening. Is it possible to determine differences in genetics and gene expression of keratinocytes involved in Oral Lichen Planus compared to those of 'normal' tissue by using biopsy material and an in vitro model of oral lichen planus?

Official Title
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Molecular & Cellular Characterisation of Oral Lichen Planus

Conditions
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Oral Lichen Planus

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient is 16 years old or over Patient is willing and able to provide valid informed consent Patient is attending the Oral Lichen Planus clinic and will be having a routine clinical biopsy taken Exclusion Criteria: Patient under 16 years old Patient is immunocompromised Patients with comorbid disease state e.g. other inflammatory disease

Ages Eligible for Study
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Minimum Age: 16 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Histological architecture | Are the histological architectural tissue changes that take place in oral lichen planus quantifiable using computer based imaging, graph theory and fractal geometry principles? | Through study completion, (maximum 12 months from the defined end of study) | 
| Comparisons with other dysplastic lesions | What is the nature of such architectural changes and what are the differences with normal, dysplastic and neoplastic epithelia | Through study completion, (maximum 12 months from the defined end of study) | 
| Statistical classifications | Is it possible to produce evidence-based statistical classification into established diagnostic classes using the proposed methodology | Through study completion, (maximum 12 months from the defined end of study) | 
| Automation of morphological features | Is it possible to automate such morphometrical analysis/classification? | Through study completion, (maximum 12 months from the defined end of study) | 
| Molecular expression of Oral Lichen Planus markers | Is it possible to determine differences in genetics and gene expression of keratinocytes involved in Oral Lichen Planus compared to those of 'normal' tissue by using biopsy material and an in vitro model of oral lichen planus? | Through study completion, (maximum 12 months from the defined end of study) |