The Effect of Protein Ingestion on the Recovery of Skeletal Muscle Function Following Eccentric Exercise.

Study Overview
=================
Brief Summary
-----------------
The present study will assess the effect of the type and timing of protein ingestion 6 days prior to, and 7 days following eccentric exercise on the time course of 1) the recovery of muscle function, 2) the recovery of perceived muscle soreness, 3) muscle metabolism.

Conditions
-----------------
Healthy

Intervention / Treatment
-----------------
* Dietary Supplement: Post exercise protein-polyphenol supplement
* Dietary Supplement: Post exercise placebo supplement
* Dietary Supplement: Pre bed protein-polyphenol supplement
* Dietary Supplement: Pre bed placebo supplement


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Men and women who are generally healthy and recreationally active Exclusion Criteria: Active cardiovascular disease or hypertension Diagnosed metabolic impairment Use of nutritional supplements Use of anti-inflammatory medications Habitual dietary protein intake of <0.8g/kg/day Musculoskeletal disorders

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Placebo supplement<br>Post exercise placebo supplement Pre bed placebo supplement | Dietary Supplement: Post exercise placebo supplement<br>* Participants will ingest an isocaloric placebo supplement after exercise. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>Dietary Supplement: Pre bed placebo supplement<br>* Participants will ingest an isocaloric placebo supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>|
| Active Comparator: Post exercise supplement<br>Post exercise protein-polyphenol supplement Pre bed placebo supplement | Dietary Supplement: Post exercise protein-polyphenol supplement<br>* Participants will ingest a post exercise protein-polyphenol supplement. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>Dietary Supplement: Pre bed placebo supplement<br>* Participants will ingest an isocaloric placebo supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>|
| Active Comparator: Pre bed supplement<br>Post exercise placebo supplement Pre bed protein-polyphenol supplement | Dietary Supplement: Post exercise placebo supplement<br>* Participants will ingest an isocaloric placebo supplement after exercise. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>Dietary Supplement: Pre bed protein-polyphenol supplement<br>* Participants will ingest a protein-polyphenol supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>|
| Active Comparator: Post exercise + pre bed supplement<br>Post exercise protein-polyphenol supplement Pre bed protein-polyphenol supplement | Dietary Supplement: Post exercise protein-polyphenol supplement<br>* Participants will ingest a post exercise protein-polyphenol supplement. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>Dietary Supplement: Pre bed protein-polyphenol supplement<br>* Participants will ingest a protein-polyphenol supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Isokinetic force production of the knee extensor muscles |  | 14 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Muscle protein synthesis |  | 14 days | 
| Thigh volume |  | 14 days | 
| Perceived muscle soreness |  | 14 days | 
| Isometric force production of the knee extensor muscles |  | 14 days |