Evaluation of Galectin-3 Levels in GCF And Saliva of Patients With Different Periodontal Diseases

Study Overview
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Brief Summary
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The aim of this study is to assess the levels of galectin-3 in the GCF and saliva in patient with gingivitis and patient with stage III periodontitis.

Detailed Description
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The aim of this study is to assess the levels of galectin-3 in the GCF and saliva in patient with gingivitis and patient with stage III periodontitis. This study will be conducted in the Oral Medicine and Periodontology department, Faculty of Dentistry- Cairo University, Egypt. Patients will be selected from the outpatient clinic of the department of Oral Medicine and Periodontology, Faculty of Dentistry-Cairo University.

Official Title
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Gingival Crevicular Fluid And Salivary Levels of Galectin-3 in Patients With Gingivitis And Patients With Stage III Periodontitis: An Observational Study

Conditions
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Periodontal Diseases

Intervention / Treatment
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* Diagnostic Test: GCF obtaining
* Diagnostic Test: saliva obtaining


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: For healthy group: individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study. For the gingivitis group: individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss. For the periodontitis group that has not received periodontal treatment in the last 6 months For the periodontitis group: individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss. Presence of a minimum of 15 natural teeth. Exclusion Criteria: Individuals with any known systemic disease. Pregnant and lactating women. Individuals that received periodontal treatment within the last 6 months. Individuals with a history of systemic antibiotics and anti-inflammatory drugs within the last 3 months. Former or current smokers.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| healthy patient<br>Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained | Diagnostic Test: GCF obtaining<br>* GCF sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)<br>Diagnostic Test: saliva obtaining<br>* Saliva sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)<br>|
| gingivitis patient<br>Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained | Diagnostic Test: GCF obtaining<br>* GCF sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)<br>Diagnostic Test: saliva obtaining<br>* Saliva sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)<br>|
| patients with Stage III periodontitis<br>Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained | Diagnostic Test: GCF obtaining<br>* GCF sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)<br>Diagnostic Test: saliva obtaining<br>* Saliva sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| galectin-3 in GCF | The total amount of Galectin-3 in GCF | 24 hours after taking the clinical measurements at the first visit | 
| galectin-3 in saliva. | The total amount of Galectin-3 in saliva | 24 hours after taking the clinical measurements at the first visit | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Pocket depth | in mm | 24 hours before taking GCF and salivary samples | 
| Clinical attachment level | in mm | 24 hours before taking GCF and salivary samples | 
| Bleeding on Probing | By gently probing the orifice of the gingival crevice, the periodontal probe will be inserted 1 to2 mm into the gingival sulcus starting at one interproximal area and moving to the other. If bleeding occurs within 10 seconds a positive finding is recorded. | 24 hours before taking GCF and salivary samples | 
| Plaque Index | Scores: 0: No plaque A film of plaque adhering to the free gingival margin and adjacent area of the tooth. The plaque may be seen insitu only after application of a disclosing solution or by using the probe on the tooth surface. Moderate accumulation of soft deposit s within the gingival pocket, or the tooth and gingival margin, which can be seen with the naked eye. Abundance of soft matter within the gingival pocket and/or on the tooth and gingival margin. | 24 hours before taking GCF and salivary samples | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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galectin-3