Antioxidant Vitaminas and Hypertrophy Adaptations to Resistance Training

Study Overview
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Brief Summary
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This investigations aims to investigate whether supplementation with antioxidant vitamins (vitamin C and vitamin E) can influence, enhancing or blocking, the hypertrophic adaptations caused by strength training in sedentary subjects.

Detailed Description
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A randomized, double-blind, placebo-control controlled trial will be conducted. In a previous session volunteers will be interviewed to determine if they meet the inclusion criteria. Will be done two weeks of flushing out other types of supplementation and strenuous physical exercise, along with familiarization with the strength training program and performance tests to be carried out during the rehearsal. Subsequently, it will be a week without exercising to start with the experimental designs. From the start of the trial, participants should avoid physical exercise. strenuous outside the training protocol and if usual, they will only be able to perform one resistance training session per week. Participants should avoid ingesting of foods that contain high amounts of antioxidant compounds: more than two juices of fruits, more than four cups of coffee or tea. Juices with high antioxidant content should be avoided. Alcoholic beverages should also be avoided. Group 1 (G1): Antioxidant vitamins (VitC (1000 mg) + VitE (235 mg)) + exercise Group 2 (G2): Placebo + exercise A supplementation period of 12 weeks will be carried out. In each period, supplementation with antioxidant vitamins: VitC (1000 mg) + VitE (235 mg) or with placebo. All participants will ingest the supplementation between 1-3 hours before training, the days of rest in the mornings. All participants will perform a 12-week strength training protocol. The training session performed in the sharp design will be the first training session in chronic design. Being the baseline (E0) and post-exercise (E1) assessment performed in G1, G2 and G3 also the first evaluation corresponding to the chronic design. In the middle of the supplementation period (week 6) and at the end, two new evaluations (week 12). Baseline evaluations will be performed prior to an exercise session of week 6 (E2) and at the last exercise session (E3) of the following parameters: performance physique, body composition, micronutrient analysis, muscle ultrasound. I also know conduct post-exercise physical performance assessments Figure 2. The strength training protocol will be carried out 4 days a week at the facilities sports of the European University. The sessions will be planned by a professional from the Sciences of Physical Activity and Sports. Sessions will be tracked through a diary.

Official Title
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Vitamins C and Vitamin E and Hypertrophy Adaptations to Resistance Traini

Conditions
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Hypertrophy

Intervention / Treatment
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* Dietary Supplement: Vitamin C and vitamin E
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Experience in strength training of at least one year Exclusion Criteria: Smokers Antioxidant supplementation Injuries or diseases

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: A randomized, double-blind, placebo-control, parallel controlled trial will be conducted.
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Supplementation group<br>Antioxidant vitamins (Vitamin C (1000 mg) + Vitamine E (235 mg)) | Dietary Supplement: Vitamin C and vitamin E<br>* 1000 mg 235 mg<br>* Other names: Ascorbic acid and tocopherol;|
| Placebo Comparator: Placebo group<br>Placebo supplementation with the same aspect as supplementation | Dietary Supplement: Placebo<br>* Placebo<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| DEXA | Evaluation of muscle mass with dual energy X-ray absorptiometry | Week 0 | 
| DEXA | Evaluation of muscle mass with dual energy X-ray absorptiometry | Week 10 | 
| Anthropometry | Evaluation of muscle mass by a ISAK 1 level anthropometrist | Week 0 | 
| Anthropometry | Evaluation of muscle mass by a ISAK 1 level anthropometrist | Week 5 | 
| Anthropometry | Evaluation of muscle mass by a ISAK 1 level anthropometrist | Week 10 | 
| Bioimpedance | Evaluation of muscle mass with an Inbody | Week 0 | 
| Bioimpedance | Evaluation of muscle mass with an Inbody | Week 5 | 
| Bioimpedance | Evaluation of muscle mass with an Inbody | Week10 | 
| DOMS | Delayed onset muscle soreness evaluated with visual analoguic scale (0 no pain- 10 maximal pain) | Week 0 | 
| DOMS | Delayed onset muscle soreness evaluated with visual analoguic scale (0 no pain- 10 maximal pain) | Week 5 | 
| DOMS | Delayed onset muscle soreness evaluated with visual analoguic scale (0 no pain- 10 maximal pain) | Week 10 | 
| Manual dinamometry | Handgrip | Week 0 | 
| Manual dinamometry | Handgrip | Week 5 | 
| Leg dinamometry | Dinamometer | Week 0 | 
| Leg dinamometry | Dinamometer | Week 5 | 
| Leg dinamometry | Dinamometer | Week 10 | 
| RPE | 10-point scale (no exertion 0- maximal effort 10) | Week 0 | 
| RPE | 10-point scale (no exertion 0- maximal effort 10) | Week 5 | 
| RPE | 10-point scale (no exertion 0- maximal effort 10) | Week 10 | 
| 1 RM | Bench press and squats | Week 0 | 
| 1 RM | Bench press and squats | Week 5 | 
| 1 RM | Bench press and squats | Week 10 | 
| Parameters determined by ENCODER | Bench press and squats | Week 0 | 
| Parameters determined by ENCODER | Bench press and squats | Week 5 | 
| Parameters determined by ENCODER | Bench press and squats | Week 10 |