Effects of Plasmapheresis on Aging Biomarkers

Study Overview
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Brief Summary
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The aim of the study is to evaluate the effect of plasmapheresis (repeated plasma withdrawals) on selected health indicators (hematological, biochemical, immunological and indicators of biological age) of plasma donors.

Official Title
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Studie Vlivu darování krevní Plazmy - Plasmapheresis. Study of the Plasma Donation Effect - Plasmapheresis

Conditions
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Aging, Plasmapheresis

Intervention / Treatment
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* Procedure: Plasmapheresis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Generally healthy volunteer subjects age 40 - 60 50:50 man and woman Exclusion Criteria: Subjects excluded from plasma donation protocols (serious illness, infection, etc) Subjects undergoing stronger anti-aging interventions

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group 1<br>Group 1 consists of first time donors undergoing all 8 tracked plasmaphereses. | Procedure: Plasmapheresis<br>* Procedure whereby plasma is separated and extracted from anticoagulated whole blood and the red cells retransfused to the donor.<br>|
| Group 2<br>Group 2 consists of first time donors undergoing only last 4 plasmaphereses, serving as control for the first 4 donations. | Procedure: Plasmapheresis<br>* Procedure whereby plasma is separated and extracted from anticoagulated whole blood and the red cells retransfused to the donor.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Significant change of biological age according to epigenetic clock after 8 plasmaphereses | Significant change of the epigenetic age of the plasma donors after 8 cycles of plasmapheresis conducted or a slower increase of the biological age throughout the study than the increase of the chronological age. | Through study completion, an average of 8 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Significant change of biological age according to epigenetic clock after 8 plasmaphereses | Significant change of the epigenetic age of the plasma donors after 4 cycles of plasmapheresis conducted or a slower increase of the biological age throughout the study than the increase of the chronological age. | Through study completion, an average of 8 months | 
| Significant change of the proteomic profile after 8 plasmaphereses | Proteomic analysis showing signs of a rejuvenated proteostasis in the plasma donors after 8 cycles of plasmapheresis. | Through study completion, an average of 8 months | 
| Significant change of the proteomic profile after 4 plasmaphereses | Proteomic analysis showing signs of a rejuvenated proteostasis in the plasma donors after 8 cycles of plasmapheresis. | Through study completion, an average of 8 months | 
| Significant change of the biological age according to a blood biochemistry clock after 8 plasmaphereses | Blood biochemistry clock showing phenotype of a younger individual in the plasma donors after 8 cycles of plasmapheresis. | Through study completion, an average of 8 months | 
| Significant change of the biological age according to a blood biochemistry clock after 4 plasmaphereses | Blood biochemistry clock showing phenotype of a younger individual in the plasma donors after 8 cycles of plasmapheresis. | Through study completion, an average of 8 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Aging, Plasmapeheresis