Examining The Effect of Blood Flow Restricted Aerobic Training on Mitochondrial Adaptations in Young Healthy Adults

Study Overview
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Brief Summary
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This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.

Official Title
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Examining The Effect of Blood Flow Restricted Aerobic Training on Citrate Synthase Activity in Young Healthy Adults

Conditions
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Mitochondrial Biogenesis

Intervention / Treatment
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* Other: BFR Exercise Training
* Other: CTL Exercise Training


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 3 hours of physical activity per week No previous cycling training No concurrent involvement in another training program Body mass index < 30 kg/m2 Exclusion Criteria: Presence of cardiovascular disease Presence of metabolic disease Taking regular oral medication Current smoker

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: BFR<br> | Other: BFR Exercise Training<br>* Participants will complete exercise training on a recumbent bike placed on a 45 degree incline (BFR).<br>|
| Experimental: CTL<br> | Other: CTL Exercise Training<br>* Participants will complete exercise training on a recumbent bike placed on flat ground (CTL).<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Citrate Synthase | Fold change in citrate synthase maximal activity | 3 days prior to start of exercise training and 3 days after completing exercise training | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Transcription factor A, mitochondrial (TFAM) | Fold change in TFAM protein | 3 days before training to 3 days after training | 
| Nuclear respiratory factor, 1 (NRF1) | Fold change in NRF-1 protein | 3 days before training to 3 days after training | 
| Maximal oxygen consumption (VO2peak) | Change in VO2peak (POST-PRE) | 3 days before training to 3 days after training | 
| Heart rate (HR) | Average HR | Every 5 minutes during each of the 7, 30-minute exercise sessions | 
| Revolutions per minute (RPM) | Average RPM | Every 5 minutes during each of the 7, 30-minute exercise sessions | 
| Blood [lactate] | Average blood lactate concentration | During all exercise sessions at 10 and 30 minutes | 
| Rating of perceived exertion (RPE) | Average RPE | Every 5 minutes during each of the 7, 30-minute exercise sessions | 
| Nuclear Factor, Erythroid 2 Like 2 (NFE2L2) | Fold change in NFE2L2 | 3 days before training to 3 days after training | 
| Cytochrome c oxidase (COX) | Fold change in COX | 3 days before training to 3 days after training | 
| Electromyography (EMG) | Median frequency and average amplitude of EMG signal | Throughout each of the 7, 30-minute exercise sessions | 
| Near-infrared spectroscopy (NIRS) | Deoxygenated hemoglobin and oxygenated hemoglobin | Throughout each of the 7, 30-minute exercise sessions | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Mitochondria, Exercise training, VO2, Aerobic exercise, Citrate Synthase, Blood flow restriction