Selecting Specific Bio-markers and Researching Mechanisms of Immune Regulation From Inhalation Injury and Traumatic Lung Injury Based on Single Cell Sequencing Technology

Study Overview
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Brief Summary
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The goal of this observational study is to profile the circulating immunological traits of patients with traumatic lung injury (TLI) and inhalation injury (ILI),providing a new direction for the depth research of the pathogenesis, and providing new immune-related targets for diagnosis of the severity of lung injury and treatment. The investigators performed scRNA-seq of neutrophil and peripheral blood mononuclear cells (PBMCs) from 10 ml fresh circulating blood from 3 patients with TLI and 3 patients with ILI at longitudinal timepoints,as well as peripheral blood from 3 health controls(HCs).

Detailed Description
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The investigators performed single-cell transcriptomic sequencing (BD Rhapsody) on neutrophil and PBMCs extracted from the fresh circulating blood from 3 patients with TLI and 3 patients with ILI at longitudinal timepoints,as well as peripheral blood from 3 health controls(HCs). Three time points are picked by clinical conditions of patients with TLI or ILI: day 1 post hospital admission, day 3 post hospital and the day before discharged which matched to acute stage,progressive stage and convalescence.

Official Title
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Selecting Specific Bio-markers and Researching Mechanisms of Immune Regulation From Inhalation Injury and Traumatic Lung Injury Based on Single Cell Sequencing Technology

Conditions
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Traumatic Lung Injury, Inhalation Injury

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Lung injury caused by burn trauma is confirmed, and the patient is hospitalized within 24 hours after burn or trauma (the clinician can make a definite diagnosis of lung injury based on the comprehensive judgment of the patient's etiology, history, clinical manifestations, blood gas analysis, laboratory tests and imaging); Patients with inhalation injury caused by head, face and neck burns or inhalation injury combined with multiple body burns < 30%; Patients with simple inhalation injury; The normal control group should have no smoking history and be normal population; According to the provisions of Good Clinical Practice(GCP), the informed consent has been obtained and the informed consent has been signed to volunteer for the test. Exclusion Criteria: Basic pulmonary diseases like chronic obstructive pulmonary disease (COPD), asthma and et al; Severe organ dysfunction; Autoimmune diseases; Tumor; Severe infection; Pregnant or nursing women; Fatal or life-threatening events caused by trauma; Any history which may interfere the results or increase the risk of patients judged by researchers.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 90 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| patients with traumatic lung injury (TLI)<br> | |
| patients with inhalation injury (ILI)<br> | |
| health controls(HCs)<br> | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Computerized tomography, CT | according to the proportion of rib fracture, pneumothorax, hemothorax,lung contusion in the image | 1day | 
| Computerized tomography, CT | according to the proportion of rib fracture, pneumothorax, hemothorax,lung contusion in the image | 3 day | 
| Computerized tomography, CT | according to the proportion of rib fracture, pneumothorax, hemothorax,lung contusion in the image | through study completion, an average of 2 weeks | 
| Partial pressure of carbon dioxide in arterial blood, PaCO2 |  | 1day | 
| Partial pressure of carbon dioxide in arterial blood, PaCO2 |  | 3 day | 
| Partial pressure of carbon dioxide in arterial blood, PaCO2 |  | through study completion, an average of 2 weeks | 
| Oxygenation index and Fraction of Inspired Oxygen will be combined to report FiO2/PaO2 |  | 1 day | 
| Oxygenation index and Fraction of Inspired Oxygen will be combined to report FiO2/PaO2 |  | 3 day | 
| Oxygenation index and Fraction of Inspired Oxygen will be combined to report FiO2/PaO2 |  | through study completion, an average of 2 weeks |