Genetic Polymorphism and Post Operative Nausea and Vomiting (PONV)

Study Overview
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Brief Summary
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Nausea and vomiting affects 25-30% of individuals in the post-operative period and can reach more than 70-80% in high-risk patients. inherited factors may play a significant role in individual susceptibility and clinical research on hereditary factors involved in the pathogenesis of Postoperative nausea and vomiting (PONV) and chemotherapy nausea and vomiting (CINV) is relatively new. The aim of this study is to investigate whether inter-individual differences related to PONV are associated with genetic factors. 300 patients will be evaluated in postoperative oncological surgeries. The peripheral leukocyte DNA will be extracted by the Salting Out Procedure method and processed to genotyping for 48 SNPs from 15 candidate genes by real-time PCR by the Taqman method. The possible associations with demographic data and factors related to surgery will be analyzed by univariate and multivariate analysis.

Detailed Description
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The aim of this study is to investigate whether inter-individual differences related to PONV are associated with genetic factors. 300 patients will be evaluated in postoperative oncological surgeries. The peripheral leukocyte DNA will be extracted by the Salting Out Procedure method and processed to genotyping for 48 single nucleotide polymorphisms (SNPs) from 15 candidate genes by real-time PCR by the Taqman method. The possible associations with demographic data and factors related to surgery will be analyzed by univariate and multivariate analysis.

Official Title
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Genetic Polymorphism Associated With the Occurrence of Postoperative Vomiting and Vomiting (PONV) in Patients Undergoing Oncological Surgeries

Conditions
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Genetic Predisposition to Disease, Postoperative Nausea, Polymorphism, Genetic

Intervention / Treatment
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* Diagnostic Test: DNA extraction


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: High and intermediate-risk patients for PONV (Apfel score 2, 3 and 4) Exclusion Criteria: Low risk patients for PONV (Apfel score 0 and 1)

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| PONV<br>Patients presenting with postoperative nausea and vomiting | Diagnostic Test: DNA extraction<br>* DNA will be extracted from white cells<br>|
| no PONV<br>Patients not presenting with postoperative nausea and vomiting | Diagnostic Test: DNA extraction<br>* DNA will be extracted from white cells<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| PONV (Post operative nausea and vomiting) | Incidence of early (6 hours) and late (24 h) nausea and vomiting | 24 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Intensity of Acute Postoperative pain | Intensity of Postoperative pain measures by numerical rate scale and descriptive scale will be assessed in the first 24 hours in all patients | 24 hours |