Lemon Verbena Extract Oxidative Stress and Muscle Damage

Study Overview
=================
Brief Summary
-----------------
In the present study, investigators investigated whether supplementation with lemon verbena extract (LVE) could improve muscle damage and biochemical indicators after exhaustive exercise challenge. Based on maximum jump heights, 60 subjects (30 males and 30 females) were equally divided into a placebo group (0 mg/human/day) and an LVE supplement group (400 mg/human/day), with gender-equal distribution. All subjects started supplementation 10 days before exhaustive exercise and continued it until all tests were completed. Before the intervention, after the exhaustive exercise, and on the following 3 days, the participants underwent 12-minute Cooper running/walking; blood collection; assessments of pain, muscle stiffness, maximum jump heights, and isometric maximum muscle strength; and anaerobic strength tests.

Official Title
-----------------
Evaluation of the Efficacy of Supplementation With Lemon Verbena Extract in Improving

Conditions
-----------------
Muscle Injury, Muscle Soreness

Intervention / Treatment
-----------------
* Dietary Supplement: Lvemon verbena extract
* Dietary Supplement: maltodextrin


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: health Must be able to swallow tablets Exclusion Criteria: non-smokers no musculoskeletal medical metabolic disease women not on the reproductive cycle high blood pressure, asthma skeletal neuromuscular injuries in the upper or lower extremities

Ages Eligible for Study
-----------------
Minimum Age: 20 Years
Maximum Age: 30 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Placebo<br>0 mg LVE/capsule, 2capsule/day, 14days | Dietary Supplement: maltodextrin<br>* Each capsule contained 0 mg of lemon verbena extract 2 capsules/day, 14 days<br>|
| Experimental: Lemon Verbena extract<br>200 mg LVE/capsule, 2capsule/day, 14days | Dietary Supplement: Lvemon verbena extract<br>* Each capsule contained 200 mg of lemon verbena extract 2 capsules/day, 14 days<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| inflammation of IL-6 level | To assess inflammation indicators, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Assess serum IL-6 levels (pg/ml) to monitor physiological fitness. Used commercial kit human IL-6 immunoassay (R&D system, Minnesota, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis | 10 days | 
| Clinical Biochemistry of CK level | To assess muscle damage, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Serum CK (U/L), were assessed for monitoring physiological adaptation. All biochemical indices were assessed using an autoanalyzer (Hitachi 7060, Tokyo, Japan). | 10 days | 
| Stress hormones 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) in Urine | Subjects' urine was collected 24 hours after the exhaustive exercise for analysis. Used commercial kit human 8-OHdG (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis | 10 days | 
| Assessment of muscle stiffness | 3 h, 24h, 48h after exhausting exercise, use Myoton machine to evaluate muscle stiffness (N/m) (Myoton, Tallinn, Estonia) | 10 days | 
| Visual Analogue Scale (VAS) evaluation | 3 h, 24h, 48h after exhausting exercise, use VAS questionnaire to record muscle pain. the minimum is 0 and maximum is 10, and whether higher scores mean more pain | 10 days | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
DOMS,, Lemon verbena