The Relationship Between Exercise Frequency, Intensity, and Restoration of Cardiometabolic Health

Study Overview
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Brief Summary
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Regular physical activity is well established to decrease the risk of cardiometabolic diseases. While research has characterized responses based on exercise intensity, many beneficial effects of exercise are transient in nature, and therefore exercise frequency may play an important, yet currently under-appreciated, role in improving health. The purpose of this study is to determine the efficacy of 6-week high-frequency endurance (END) or low-frequency sprint (SIT) training with respect to reducing clinically relevant cardiometabolic risk factors in overweight/obese males. It is hypothesized that END, performed at a greater frequency than SIT, will markedly improve cardiometabolic health, while low-frequency SIT will not.

Detailed Description
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Involvement in regular physical activity is known to elicit systemic adaptations and reduce the risk of cardiometabolic diseases, including hypertension, obesity, dyslipidemia, and hyperglycemia. Traditional physical activity recommendations suggest that 150 minutes of moderate-intensity continuous endurance (END) exercise dispersed over 5 days per week is sufficient to improve physical fitness in adults. However, given the commonly cited barrier of lack of time, literature has recently focused on time effective sprint interval training (SIT), obtaining equivalent increases in aerobic capacity and acute glycemic regulation compared to classical END exercise when protocols are work-matched. Despite these similarities, END is conducive to daily sessions not feasible of SIT. As improvements in many clinically relevant risk factors are transient in nature following exercise, it remains imperative to assess the implications of variable frequency exercise regimes performed as per general practice (i.e. high-frequency END, low-frequency SIT). Furthermore, improvements in cardiovascular outcomes following END have been shown, in some instances, to be absent in response to SIT, suggesting END may be more beneficial for cardiovascular health. Therefore, the current study aims to assess several markers of cardiovascular (aerobic capacity, blood pressure, arterial stiffness, vascular endothelial function) and metabolic (glucose tolerance, lipid tolerance, body composition) health following 6-weeks of high-frequency END or low-frequency SIT, performed as per general practice. Combined, this research will provide important insight into the under-appreciated role of exercise frequency for improving cardiometabolic health.

Official Title
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The Relationship Between Exercise Frequency, Intensity, and Restoration of Cardiometabolic Health

Conditions
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Overweight or Obesity

Intervention / Treatment
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* Behavioral: Endurance Exercise Training (END)
* Behavioral: Sprint Exercise Training (SIT)


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male, aged 18-70 years Body mass index (BMI) > 25 kg/m^2 (classified as overweight or obese) Sedentary (<100 minutes moderate physical activity per week) Approval for vigorous exercise via physical activity readiness questionnaire (PARQ+) Exclusion Criteria: Prescribed with glucose lowering medications Smoker Not cleared for physical activity

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: This study is a 6-week exercise training study, with two possible parallel training groups: endurance (END) or sprint (SIT) training.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Endurance Exercise Training (END)<br>This group is performing END training for 6 weeks in duration. Intervention: Behavioral: Endurance Exercise Training (END) | Behavioral: Endurance Exercise Training (END)<br>* Physical activity will be conducted on cycle ergometers under supervision. Participants will exercise 5 days a week for 30 minutes (Week 1-2); 35 minutes (Weeks 3-4); or 40 minutes (Weeks 5-6) at 60% VO2 peak.<br>|
| Experimental: Sprint Exercise Training (SIT)<br>This group is performing SIT training for 6 weeks in duration. Intervention: Behavioral: Sprint Exercise Training (SIT) | Behavioral: Sprint Exercise Training (SIT)<br>* Physical activity will be conducted on cycle ergometers under supervision. Participants will exercise 3 days a week involving a 3-minute warm-up, followed up 4 repetitions (Week 1-2); 5 repetitions (Weeks 3-4); or 6 (Weeks 5-6) repetitions of 30 seconds at a maximal intensity with 2 minutes' rest in between. Exercise will conclude with a 2-minute cool-down.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Cardiorespiratory fitness | Assessed via VO2 peak test, to determine the change in cardiorespiratory fitness following 6-weeks of exercise training | Baseline (pre-training) vs. week 6 (post-training) | 
| Free-living glycemic regulation | Assessed via continuous glucose monitoring (CGM), to determine the change in free-living glycemic regulation following 6-weeks of exercise training | Baseline (pre-training) vs. week 6 (post-training) | 
| Glucose tolerance | Assessed via an oral glucose tolerance test (OGTT) to determine changes in standardized glycemic regulation following 6-weeks of exercise training | Baseline (pre-training) vs. week 6 (post-training) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood lipids | Blood lipid profile from fasted venous blood sampling, including high-density lipoproteins (HDL), low-density lipoproteins (LDL), high-sensitivity C-reactive protein (Hs-CRP), cholesterol, non-HDL cholesterol, triglycerides (TAG), free-fatty acids (FFA), and cholesterol/HDL ratio | Baseline (pre-training) vs. week 6 (post-training) | 
| HbA1C | Change in HbA1C assessed via fasted venous blood sampling, following 6-weeks of exercise training | Baseline (pre-training) vs. week 6 (post-training) | 
| Post-prandial blood lipids | Assessed following the consumption of an oral fat tolerance test (OFTT). Blood lipid responses include triglycerides (TAG) and free fatty acids (FFA), assessing the influence of 6-weeks of exercise training on these parameters | Baseline (pre-training) vs. week 6 (post-training) | 
| Blood pressure | Assessed via automated brachial blood pressure (including systolic (SBP), diastolic (DBP), and mean arterial pressure (MAP)) | Baseline (pre-training) vs. week 6 (post-training) | 
| Body composition | Assessed via dual-energy X-ray absorptiometry (DXA); including total and regional lean and fat mass. Assessed via height and weight measurements to determine BMI. | Baseline (pre-training) vs. week 6 (post-training) | 
| Arterial stiffness | Assessed via carotid-femoral pulse wave velocity (PWV) | Baseline (pre-training) vs. week 6 (post-training) | 
| Arterial stiffness acutely post-exercise | Assessed via carotid-femoral pulse wave velocity (PWV) following a single bout of exercise in week 1 of each group | Acutely pre-exercise vs. post-exercise in week 1 of training | 
| Brachial artery vascular function | Assessed via brachial artery flow mediated dilation (FMD) following 6-weeks of exercise training | Baseline (pre-training) vs. week 6 (post-training) | 
| Brachial artery vascular function acutely post-exercise | Assessed via brachial artery flow mediated dilation (FMD) following a single bout of exercise in week 1 of each training group | Acutely pre-exercise vs. post-exercise in week 1 of training | 
| Daily sedentary/active time | Assessed via accelerometer | Baseline (pre-training), week 1 (of training), week 6 (post-training) | 
| Free-living glycemic regulation during the first week of exercise training | Assessed via continuous glucose monitoring (CGM) | Baseline (pre-training) vs. week 1 (of training) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Endurance training, Sprint interval training, Glycemic regulation, Lipid homeostasis, Cardiovascular health