Proteomics for Chronic Disorder of Consciousness

Study Overview
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Brief Summary
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Following severe traumatic brain injury, patients may remain unconscious for many years. It is the first proteomic analysis comparing differentially expressed proteins between patients with chronic disorder of consciousness and controls so far. The investigators generated accurate lists of proteomes and identified differences at different disease time courses. The obtained results provide new insight into understanding the molecular mechanisms underlying the chronic disorder of consciousness.

Detailed Description
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Background: Patients who awake from severely traumatic brain injury (sTBI) may remain unconscious for many years. Although behavioral assessment and functional imaging are currently used as diagnostic tools, the molecular basis underlying chronic condition has yet to be explored. Objective:To explore the molecular basis and identify novel biomarkers for the diagnosis and prognosis of patients with chronic disorder of consciousness. Method: Plasma samples were obtained from patients with chronic disorder of consciousness, and healthy volunteers. A coupled isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach was used to screen differentially expressed proteins (DEPs) between patients and controls. Potential molecular mechanisms were further discussed through bioinformatics analyses. Candidate proteins were validated by enzyme-linked immunosorbent assay (ELISA).

Official Title
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iTRAQ-Based Proteomics Reveals Potential Biomarkers for Chronic Disorder of Consciousness

Conditions
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Disorder of Consciousness

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients ages 14 to 65 years old; Patients had a history of sTBI; Patients had awakened from a coma (indicating preserved sleep-wake cycles), including vegetative state and minimally consciousness state; Patients stay unconscious for more than 1 month. Exclusion Criteria: Patients with acute infectious diseases or liver dysfunction; Patients with serious diseases such as cardiac or pulmonary problems.

Ages Eligible for Study
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Minimum Age: 14 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Protein profiling | A coupled isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach was used to screen differentially expressed proteins (DEPs) between patients and controls. | Three months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glasgow Outcome Scale (GOS) | A GOS value of <3 was considered as a bad recovery, while a GOS value of ≥3 was considered as a good recovery. | In one year | 
| Coma Recovery Scale-Revised(CRS-R) | Coma Recovery Scale-Revised (CRS-R) score was used to quantify the severity, which consists of 23 hierarchically arranged items that comprise six subscales addressing auditory, visual, motor, oromotor/verbal, communication, and arousal processes. The lowest item on each subscale represents reflexive activity, whereas the highest items represent cognitively mediated behaviors. | 30 minutes before blood collection | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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proteomics, plasma, chronic disorder of consciousness