Efficacy Testing of Collagen Elixir

Study Overview
=================
Brief Summary
-----------------
To assess Isagenix-Collagen Elixir on skin condition improvement

Official Title
-----------------
Efficacy Testing of Collagen Drink

Conditions
-----------------
Skin Condition

Intervention / Treatment
-----------------
* Dietary Supplement: Collagen drink
* Dietary Supplement: Blank


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Healthy adults aged above 20 years old Exclusion Criteria: Subject who is not willing to participate in this study. Patients with diseases of the skin, liver, kidney. Subjects who have known cosmetic, drug or food allergies, difficulty in digestive tract absorption or disorder. Female who is pregnant or nursing or planning to become pregnant during the course of the study. Received facial laser therapy, chemical peeling or UV overexposure in the past 4 weeks. Constant drug use Participated cosmetic product human study in the past 3 months.

Ages Eligible for Study
-----------------
Minimum Age: 20 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Placebo drink<br> | Dietary Supplement: Blank<br>* Placebo drink<br>|
| Experimental: Collagen drink<br> | Dietary Supplement: Collagen drink<br>* Testing product<br>* Other names: Isagenix-Collagen Elixir;|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The change of skin wrinkles | VISIA Complexion Analysis System was utilized to measure skin wrinkles. Units: arbitrary units | Change from Baseline skin wrinkles at 4 weeks | 
| The change of skin texture | VISIA Complexion Analysis System was utilized to measure skin texture. Units: arbitrary units | Change from Baseline skin texture at 4 weeks | 
| The change of skin collagen density | DermaLab® Series SkinLab Combo was utilized to measure skin pores. Units: arbitrary units | Change from Baseline skin collagen density at 4 weeks | 
| The change of skin elasticity | Soft Plus was utilized to measure skin elasticity. Units: arbitrary units | Change from Baseline skin elasticity at 4 weeks | 
| The change of MMP-1 of blood | Venous blood was sampled to measure MMP-1 | Change from Baseline MMP-1 at 4 weeks | 
| The change of TIMP-1 of blood | Venous blood was sampled to measure TIMP-1 | Change from Baseline TIMP-1 at 4 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The change of skin melanin index | Soft Plus was utilized to measure skin melanin index. Units: arbitrary units | Change from Baseline skin melanin index at 4 weeks | 
| The change of skin L* value | Chroma Meter MM500 was utilized to measure skin L* value. Units: arbitrary units, 0-100 | Change from Baseline L* value at 4 weeks | 
| The change of skin moisture | Corneometer® CM825 was utilized to measure skin moisture. Units: arbitrary Corneometer® units 0-120 | Change from Baseline skin moisture at 4 weeks | 
| The change of skin spots | VISIA Complexion Analysis System was utilized to measure skin spots. Units: arbitrary units | Change from Baseline skin spots at 4 weeks | 
| The change of skin UV spots | VISIA Complexion Analysis System was utilized to measure skin UV spots. Units: arbitrary units | Change from Baseline skin UV spots at 4 weeks | 
| The change of skin brown spots | VISIA Complexion Analysis System was utilized to measure skin brown spots. Units: arbitrary units | Change from Baseline skin brown spots at 4 weeks | 
| The change of TNF-α of blood | Venous blood was sampled to measure TNF-α | Change from Baseline TNF-α at 4 weeks | 
| The change of IL-10 of blood | Venous blood was sampled to measure IL-10 | Change from Baseline IL-10 at 4 weeks | 
| The change of SOD-RBC of blood | Venous blood was sampled to measure SOD-RBC | Change from Baseline SOD-RBC at 4 weeks | 
| The change of f-thiols of blood | Venous blood was sampled to measure f-thiols | Change from Baseline f-thiols at 4 weeks | 
| The change of t-GSH of blood | Venous blood was sampled to measure t-GSH | Change from Baseline t-GSH at 4 weeks | 
| The change of GST-RBC of blood | Venous blood was sampled to measure GST-RBC | Change from Baseline GST-RBC at 4 weeks | 
| The change of MDA of blood | Venous blood was sampled to measure MDA | Change from Baseline MDA at 4 weeks | 
| The change of blood total antioxidant capacity (TAC) | Venous blood was sampled to measure concentrations of TAC | Change from Baseline TAC at 4 weeks |