Topical Agents Containing Magnesium Sulfate & Wound Healing in the Rat Model

Study Overview
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Brief Summary
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An experimental study examining wound healing in 5 different groups on 30 rats.

Detailed Description
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Magnesium topically increases collagen synthesis and angiogenesis, providing faster and higher quality wound healing. In addition, with its analgesic effect, it will also eliminate the pain sensation caused by the wound and increase the quality of life of the patient whose skin integrity is impaired.

Official Title
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The Effect of Topical Agents in Cream Formations Containing Magnesium Sulfate on Wound Healing in the Rat Model

Conditions
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Wound Heal

Intervention / Treatment
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* Other: placebo comparator
* Other: sham comparator
* Other: Experimental drug 1
* Other: Experimental drug 2
* Drug: Centella asiatica


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy rats between ages of 8- 12 weeks Exclusion Criteria: weight loss of more than 15% of body weight

Ages Eligible for Study
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Minimum Age: 8 Weeks
Maximum Age: 12 Weeks

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Group placebo<br>Isotonic solution will be administered daily to 6 female rats. | Other: placebo comparator<br>* solution containing 0.9 % sodium chlorine<br>|
| Sham Comparator: Group sham<br>Base cream to be applied twice a day to 6 female rats. | Other: sham comparator<br>* vaseline, beeswax,polyglyceryl-3 diisostearate, sorbitan caprylate, propandiol,benzoic acid, glycerin, water<br>|
| Experimental: Group %1 mgso4<br>Cream containing 1% MgSO4 will be applied twice a day to 6 female rats. | Other: Experimental drug 1<br>* A cream containing 10 % MgSO4.vaseline, beeswaxpolyglyceryl-3 diisostearate, sorbitan caprylate, propandiol,benzoic acid, glycerin, water<br>|
| Experimental: Group %10 MgSO4<br>Cream containing 10% MgSO4 will be applied twice a day to 6 female rats. | Other: Experimental drug 2<br>* A cream containing 10 % MgSO4.vaseline, beeswax,polyglyceryl-3 diisostearate, sorbitan caprylate, propandiol,benzoic acid, glycerin, water<br>|
| Active Comparator: Group positive control<br>Cream containing centella asiatica will be applied twice a day to 6 female rats. | Drug: Centella asiatica<br>* A cream containing Centella asiatica<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 7th day evaluation | Histopathological evaluation of wound healing comparing with the rate of vascular endothelial growth factor (VEGF), transforming growth factor (TGF-beta) and hydroxyproline at immunohistochemical staining with biopsy on the 7th day. | 7th day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 15th day evaluation | Histopathological evaluation of wound healing comparing with the rate of VEGF, TGF-beta and hydroxyproline at immunohistochemical staining with biopsy on the 15th day. | 15th day | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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magnesium sulphate, wound healing rat model, hydroxyproline