Signaling Pathway Activation After Exercise in Patients With Chronic Obstructive Pulmonary Disease

Study Overview
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Brief Summary
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Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD). Although resistance exercises increase strength and muscle mass in patients with COPD, the response to training appears to be suboptimal in these individuals. A dysregulation in the signaling pathways involved in the regulation of muscle mass could play an important role in this phenomenon. Hypothesis: Proteins involved in muscle mass regulation will be less activated in the quadriceps of patients with COPD following the acute bout of resistance training exercise compared to healthy age-matched controls.

Detailed Description
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Our objective is to investigate the impact of an acute bout of resistance training on key signaling pathways involved in the regulation of muscle mass in moderate to severe COPD (FEV1 under 60% of predicted). Key proteins of signaling intramuscular pathways involved in protein synthesis and degradation will be measured before after 72 contractions of the quadriceps. The exercise protocol was designed to match the actual exercise prescription model use in the context of pulmonary rehabilitation. A squat, leg press and leg extension will be done at 80% of max with one minute rest between sets. A biopsy of the quadriceps will be done before and after exercise in order to measure phosphorylated kinases, total protein content and mRNA expressions. All our patients will undergo a thorough baseline assessment in pulmonary capacity, muscle force and body composition.

Official Title
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Signaling Pathway Activation in the Quadriceps of Patients With COPD After an Acute Bout of Resistance

Conditions
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Chronic Obstructive Pulmonary Disease

Intervention / Treatment
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* Other: exercise


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male COPD with an FEV1 of under 60% of predicted non-smoker Between 50 and 75 years old Exclusion Criteria: All inflammatory disease (HIV, Cancer, renal and cardiac deficiency) Hormonal dysregulation Inferior limb pathology Neuromuscular pathology History of tabacco or alcool abuse Oxygen dependent Recent exacerbation (2 months) of the symptoms of COPD

Ages Eligible for Study
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Minimum Age: 50 Years
Maximum Age: 75 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: exercise|resistance training exercise, 1 session, 3 exercises, 80% of max|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Post-exercise signaling proteins phosphorylation level in the quadriceps of COPD patients and age-matched healthy controls | Phosphorylation status of key proteins (Akt, p70, mTOR, p38, JNK, ERK) will be lesser modulated in the quadriceps of patients with COPD compare to healthy controls. The phosphorylated as well as the total protein levels will be measured by western blot. The data will be presented as arbitrary units and compared with values obtained in healthy age-matched healthy controls. | 2 hours post-exercise | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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COPD, exercise training