Oxidative Stress in OR Personnel

Study Overview
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Brief Summary
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The purpose of this study is investigate to the effect(s) of chronic occupational exposure to N20 and volatile anesthetics on the extent of oxidative stress (DNA damage and the generation of the biochemical markers) in operating room staff, with different levels of exposure to halogenated hydrocarbons, in comparison to unexposed controls (Intensive Care Staff), and to determine the relationship between the degree of oxidative stress and psychological stress, as assessed by validated psychometric measures.

Detailed Description
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This is a single blinded randomized, controlled and prospective clinical trial comparing oxidative stress status in two groups. Subjects in two groups will be matched for gender, age, smoking habit and employment duration and will be randomized into one of the two groups of: Pediatric OR Staff, and control (e.g; ICU staff) Biomarkers of oxidative stress will be measured in two different time points in each subject, (Monday Morning and, Friday afternoon of that same week->40 hours in Operating room or Intensive Care Unit). The relationship between oxidative stress and psychological stress will also be assessed by validated psychometric measures.

Official Title
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Oxidative Stress Status During Anesthesia in Pediatric Surgical Room Staff Versus Non-operating Room Personnel: Pilot Study

Conditions
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Oxidative Stress

Intervention / Treatment
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* Other: Exposure to nitrous oxide or other volatile anesthetics


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: CHOR-Staff who work > 40 hours per week in the operating rooms (Anesthesiologists, CRNA, Surgeons, Nurses) NOR- Intensive Care Unit Staff who work > 40 hours per week in Intensive care unit (Physicians, Nurses, APN'S) Exclusion Criteria: Staff with the following history will be excluded: Therapeutic exposure to radiation On chemotherapeutic agents History of General Anesthesia during the past two years
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Pediatric Operating Room (OR) Staff<br>OR Staff exposed to nitrous oxide, and other volatile anesthetics | Other: Exposure to nitrous oxide or other volatile anesthetics<br>* No intervention, but routine work-place exposure to volatile anesthetics<br>|
| Pediatric Intensive Care Unit (ICU) Staff<br>ICU Staff unexposed to any volatile anesthetics | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oxidative Stress | The extent of oxidative stress (DNA damage and the generation of the biochemical markers) in operating room staff with different levels of exposure to halogenated hydrocarbons, in comparison to unexposed controls. | During exposure to volatile anesthetics over the course of a week in the work-place | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Psychological Stress | To determine the relationship between oxidative stress and psychological stress, as assessed by validated psychometric measures. | During exposure to volatile anesthetics over the course of a week in the work-place |