CD64 and Antibiotics in Human Sepsis

Study Overview
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Brief Summary
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The purpose of the study is to find out whether CD64 expression on neutrophils measured by a new bedside test (LeukoDx) within 30 minutes is associated with effective antibiotic therapy in critically ill adult patients at risk of sepsis.

Detailed Description
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In the present study, in critically ill patients with suspected infection undergoing application of antibiotics, CD64 and inflammatory plasma markers will be determined on days 1 to 4. CD64 expression on the surface of neutrophils will be determined by fluorescence activated cell sorter (FACS) and with a new bedside test (LeukoDx). The purpose of the study is to clarify: Is CD64 expression on neutrophils associated with effective antibiotic therapy in critically ill adult patients at risk of sepsis? Is CD64 expression associated with distinct plasma parameters of inflammation? Do the results of the gold standard CD64 determination by FACS correlate with those of a new bedside test (LeukoDx)?

Official Title
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Monitoring CD64 on Neutrophils Regarding Effects of Antibiotics in Patients With SIRS Developing Sepsis

Conditions
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Antibiotic Resistant Infection, Critical Illness, SIRS, Sepsis

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age > 18 years critically ill adult patients sepsis SIRS initiation of antibiotic treatment patients < 48 hours after admission on ICU Exclusion Criteria: multiple admissions on ICU, (>1 in last 2 weeks period) leukopenia < 1 G/l and thrombocytopenia rhG-CSF or IFN-gamma therapy up to 1 week before inclusion participation in another study receiving drugs or biological within the preceeding 30 days recent longterm corticosteroid treatment HIV patients after organ transplantation treated with immunomodulating drugs pregnant patients or after delivery life expectancy < 24 hours polytraumatized patients with reanimation on scene, or infest prognosis patients under high dose corticosteroids or chemotherapy

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| CD64 expression on neutrophils measured by LeukoDx |  | 1 year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Association of course of CD64 expression with effective antibiotic treatment |  | 1 year | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Sepsis, CD64 antigens, Biomarkers, Granulocyte Colony-Stimulating Factor, Interleukin-4, Interferon-gamma, C-reactive protein, lipopolysaccharide-binding protein, procalcitonin, Cytokines, Patients, Human, Systemic Inflammatory Response Syndrome, Infection, Antibiotics, Intensive Care Units, Point-of-Care Systems, Bedside Testing