TISSUE SHRINKAGE DEPENDS ON FORMOL SOLUTION IN DIFFERENT GASTRIC LOCATIONS

Study Overview
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Brief Summary
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Generally % 10 formol solution is use to do fixation to all specimens after the surgical operations for the pathological evaluation. It is known that the formol solution cause amount of shrinkage in the tissue volume . This effect of formol solution had researched in colon,breast and prostate previously. But there is no evidence about stomach. The purpose of this research is that find out the formol effect to the gastric tissue and detect the shrinkage percent.

Detailed Description
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All gastric specimens which removed after Laparoscopic Sleeve Gastrectomy will open from the major curvature and spread down to the table.in operating room..3 cm x 3 cm three tissue samples will collect from the specific locations. These three locations are ; fundus( 1 cm from the top of staple line) , corpus (middle of the major curvature , antrum ( 1 cm far from the bottom of staple line ). All these points 5 mm far from the major curvature. 6 samples will prepare totally. 2 samples for each location ; one mucosa and one all layer of gastric wall for each location .These 3 points will examine about wall thickness(mucosa- submucosa-muscularis propria -serosa ) After saving the measurements in operating room;the gastric specimen will deliver to a pathologist to put the specimen into the % 10 formol solution. After 24 hours ;pathologist will save all of new measurements same in operating room. The measurements of sample are ;height , length , width . After the completion of the measurements the investigator will compare the results found in operating room in fresh specimen with pathologist 's results after formol. After that the investigator will be able to decide the shrinkage of gastric tissue in % 10 formol solution.

Official Title
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TISSUE SHRINKAGE DEPENDS ON FORMOL SOLUTION IN DIFFERENT GASTRIC LOCATIONS

Conditions
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Shrinking Stomach

Intervention / Treatment
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* Procedure: shrinkage


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients older than 18 years old who will undergo to Laparoscopic Sleeve Gastrectomy Exclusion Criteria: Patients who has to undergo another bariatric surgery type peroperatively Deformation of gastric specimen while removing from the abdomen

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Gastric specimen measurements after 24 hours from % 10 formol<br>Gastric specimens of patients who will undergo to Laparoscopic Sleeve Gastrectomy ,will examine after formol solution | Procedure: shrinkage<br>* shrinkage of gastric specimen in %10 formol solution after Laparoscopic Sleeve Gastrectomy<br>|
| Active Comparator: Gastric specimen measurements after surgery in operating room<br>Gastric specimens of patients who will undergo to Laparoscopic Sleeve Gastrectomy ,will examine after surgery in operating room peroperatively | Procedure: shrinkage<br>* shrinkage of gastric specimen in %10 formol solution after Laparoscopic Sleeve Gastrectomy<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| shrinkage in gastric tissue | shrinkage of gastric specimen in %10 formol solution after Laparoscopic Sleeve Gastrectomy | 24 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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shrinkage, formol, stomach, gastric wall