Protozoans in Biofilm From Patients With Periodontitis

Study Overview
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Brief Summary
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The buccal cavity is colonized by numerous microorganisms whose the number and composition could be modified with medical background (diseases and drugs) and the level of oral hygiene of the patients. Among all microorganisms identified in the periodontium, few of them are implicated in the etiopathogenesis of periodontal pathologies. To date, four major bacteria are identified for their ability to degrade periodontal tissues. Although the periodontitis is established to be the consequence of bacterial virulence and immune dysfunction, these factors fail to explain the refractory periodontitis of some patients to etiologic treatment . Others microorganisms such as protozoans could have an impact on this disease.

Official Title
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Multicentric Clinical Trials on the Prevalence of Entamoeba Gingivalis and Its Associated Bacterial Flora in Patients With Periodontitis

Conditions
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Periodontitis, Adult

Intervention / Treatment
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* Other: non applicable


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adults > 18 years-old consulting in periodontal department of CHRU Patients with moderate to severe periodontitis At least 2 periodontal sites with PPD ≥ 5mm and one healthy site No scaling root planing in the last 6 months Patients insured under the Franch social security system Exclusion Criteria: Pregnants patients Patients with antibiotic therapy and/ or all medecines which could modify the buccal microbiota in the last 6 months Tooth with endodontics periapical lesion Patients having a scaling root planing in the last 6 months Patients with guardianship

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Other: non applicable|non applicable|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| presence of protozoans | identification of protozoans by molecular technique (PCR) - | 6 months after the last inclusion: DNA samples were frozen and identified later. | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Periodontal pocket depth (PPD in mm) | Measure of PPD thanks to periodontal probe | One day: measure realised during examination of the patient for periodontal treatment. | 
| Clinical attachment Loss (CAL in mm) | Measure of CAL with periodontal probe | One day: measure realied during clincal examination of patient. | 
| Plaque and gingival index | Measure of plaque and gingival index (Loe and Silness, Silness and Loe) | One day: measure of index during the clinical examination of patient. | 
| Mobility of tooth | Measure of tooth mobility: Yes or not | One day: measure of tooth mobility during the clinical examination of patient. | 
| Bleeding On Probing (BOP) | Measure of BOP : Yes or not | One day: measure of BOP during the clinical examination of patient. |