Study of Coagulation Activation Markers and Pre Eclampsia

Study Overview
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Brief Summary
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The primary purpose of the trial is to evaluate coagulation activator markers in patients with pre eclampsia compared to control (normal pregnancy) and to correlate these markers with the severity of the pathology.

Detailed Description
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100 pre-eclamptic patients will be compared to 200 control patients (100 control matched on gestational age at inclusion and 100 control matched on delivery mode (section). Blood and urine samples will be collected at PE diagnosis, delivery and post partum. Two axes will be considered: Thrombography, or kinetic measurement of thrombin generation, by studying the coagulant profile according to Hemker's method in CAT System (Calibrated Automated Thrombogram) and by thromboelastogram in ROTEM technique (delocalized coagulation analyzer), in parallel to specific activation markers (thrombin-antithrombin complex, fibrin monomers). The balance of prostacyclin/thromboxane A2 by using ELISA method for urine samples and genotype-phenotype correlation (Polymorphism of prostacyclin-synthetase's promoter CYP8A1).

Official Title
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Coagulation Activation Markers and Pre Eclampsia : Correlation With Complications

Conditions
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Pre Eclampsia

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 years old diagnosis of pre eclampsia Exclusion Criteria: multiple pregnancy less than 18 year old

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Endogenous thrombin potential | comparison of Endogenous thrombin potential between patients with Preclampsia (PE) (at the moment of the diagnosis of PE) compared to control (at the same gestational age) | at preeclampsia diagnosis | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| genotype-phenotype correlation (Polymorphism of prostacyclin-synthetase promoter CYP8A1 |  | at pre eclampsia diagnosis | 
| In preeclampsia group : correlation between biological markers and severity of the disease | correlation of endogenous thrombin potential and rotem results with : presence or absence of severe preeclampsia presence or absence of HELLP Syndrome presence or absence of IUGR presence or absence of eclampsia | at the diagnosis of preeclampsia | 
| evolution of endogenous thrombin potential in women with preeclampsia | Study of the evolution of endogenous thrombin potential during the time period of diagnosis of preeclampsia to day 2 of the post partum. | between the diagnosis of preeclampsia and day 2 of the post partum period | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Pre eclampsia, Endogenous thrombin potential