Mitochondrial Function in Circulating Cells and Muscle Tissue

Study Overview
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Brief Summary
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The main goal of this project is to determine whether mitochondrial function in circulating cells is related to that measured in the muscle fibers of the same subjects.

Detailed Description
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Data from human and animal models strongly suggests that reduced tissue mitochondrial function can be an early marker for detection of dysfunctional metabolism. It is unclear if tissue mitochondrial dysfunction can be detected using less invasive methodology. In this project, the investigators plan to study mitochondrial bioenergetics in circulating cells and paired muscle biopsies in two metabolic disparate groups of women; healthy lean vs. overweight/obese with/without insulin resistant women of similar age. Women of similar age that are either healthy lean or overweight/obese with/without insulin resistance will be recruited to participate in one study that includes a blood draw and a muscle biopsy. Mitochondrial function in circulating blood cells and muscle tissue will be determined. The association between circulating blood cells and muscle tissue mitochondria respiration will be determined for the two groups combined, and differences between groups will also be investigated.

Official Title
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Mitochondrial Function in Circulating Cells and Muscle Tissue

Conditions
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Healthy, Obesity, Insulin Sensitivity, Women

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Women 25-35 years of age Either healthy lean (BMI<25) or insulin resistant and obese (BMI >30) Exclusion Criteria: Medication that can affect study outcomes, as determined by the principal investigator, including anti-diabetic medication Chronic diseases, acute inflammation, chronic renal failure (glomerular filtration rate <45), or heart problems

Ages Eligible for Study
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Minimum Age: 25 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Healthy lean<br>Healthy lean women, 25-35 years | |
| Overweight/obese with/without IR<br>Overweight/obese with/without insulin resistant women, 25-35 years | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Mitochondrial Function in Circulating Cells and Muscle Tissue in Young Women | Skeletal muscle (m.vastus lateralis) and peripheral blood mononuclear cell (PBMC) mitochondrial respiration were determined in response to substrate inhibitor coupling protocols using high resolution respirometry. From this, mitochondrial respiration in leak and coupled states, and reserve respiratory capacity were determined in mitochondria from skeletal muscle and from PBMCs. It was determined whether mitochondrial function in circulating cells is related to that measured in the muscle tissue of the same subjects. | During the visit, approximately 0.5 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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muscle, Mitochondria, women, PBMC