Sugar-replacement Sweeteners, and Blood Sugar Control

Study Overview
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Brief Summary
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Several sugar-replacement sweeteners are currently on the market, including saccharine (ex. Sweet'N Low), aspartame (ex. Equal), and sucralose (ex. Splenda). The purpose of this study is to examine wether non caloric sweeteners affects how well the body works to control blood sugar. The study includes detailed blood sugar testing after drinking liquids that may contain sucralose. The investigators hypothesize that drinking liquids with sucralose will effect the amounts of specific appetite-affecting substances naturally produced by the body.

Official Title
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Regulation of Incretin Release by Non-nutritive Sweeteners in Humans

Conditions
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Obesity

Intervention / Treatment
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* Other: Water
* Other: Sucralose


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: body mass index (BMI) over 30. Homeostasis model assessment of insulin resistance (HOMA-IR) < or = 2.6 Exclusion Criteria: smoking or smoked within last six months pregnant or breastfeeding have malabsorptive syndromes intestinal inflammatory disease diabetes liver or kidney disease fructose intolerance consuming more than one can of diet beverage or one spoonful of non-nutritive sweeteners (e.g. sucralose, aspartame, or saccharine) a week. taking any medication that might affect metabolism or taste.

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: water-sucralose<br>Subjects in this group drank water 10 min before drinking a glucose load on their first oral glucose tolerance test (OGTT) and drank sucralose 10 min before drinking a glucose load on their second OGTT. The two OGTT were separated on average by 1 week (i.e. washout was approximately 1 week). | Other: Water<br>* 60 ml of water were drank 10 min before doing a OGTT<br>Other: Sucralose<br>* 60 ml of 2 millimolar sucralose were drank 10 min before doing a OGTT<br>|
| Experimental: sucralose-water<br>Subjects in this group drank sucralose 10 min before drinking a glucose load on their first OGTT and drank water 10 min before drinking a glucose load on their second OGTT. The two OGTT were separated on average by 1 week (i.e. washout was approximately 1 week). | Other: Water<br>* 60 ml of water were drank 10 min before doing a OGTT<br>Other: Sucralose<br>* 60 ml of 2 millimolar sucralose were drank 10 min before doing a OGTT<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The Effect of Sucralose on Insulin Concentration (Area Under the Curve; AUC) | we will measure plasma insulin concentrations during a 5-hour modified Oral Glucose Tolerance Test (mOGTT) administered 10 minutes after subjects consume sucralose in water or an equal volume of water without sucralose (control condition).Plasma insulin concentrations were measured at 20, 15, 10, 6, and 2 min before and at 10, 20, 30, 40, 60, 90, 120, 150,180, 240, and 300 min after ingesting 75g of glucose. All these data collected were used to create the AUC curve. | Baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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obesity, diabetes, artificial sweeteners