Non Surgical Periodontal Treatment Effect on Il-17 and Il-18 in Aggressive Periodontitis

Study Overview
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Brief Summary
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clinical parameters and IL-17 and Il-18 GCF levels were measured in 25 aggressive periodontitis patients compared to 25 periodontally healthy individuals. It was observed that that Clinical parameters and Il-17 and Il-18 levels are higher before treatment but decreased after treatment which suggests role of these interleukins in pathogenesis of aggressive periodontitis .

Detailed Description
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Aggressive periodontitis is a type of rapidly progressing periodontal diseases .The three classic primary characteristics of this disease are ; occurring in young healthy patients with rapid attachment loss and familial aggregation .Bacteria-host interactions, insufficiencies in host defenses and possibly a genetic predisposition represent the etiologic factors . Beside the young age of onset, important factors as involvement of host factors and non-plaque-related etiologic factors have also been proposed . Aim of the study: Objectives were to compare and correlate the clinical parameters and biochemical parameters (Il-17 and Il-18 levels in GCF) of aggressive periodontitis patients (At baseline and after three months of nonsurgical treatment) versus periodontally healthy subjects at baseline. Subjects and methods: Populations included in the study: A total of fifty individuals were enrolled and categorized as; group A that included twenty five aggressive periodontitis patients and group B which included twenty five periodontally healthy controls. Clinical parameters: Plaque index (PI) ,gingival index (GI), Probing pocket depth (PPD) and clinical attachment level (CAL) were measured at baseline for both groups and after three months of non surgical periodontal treatment completion for group A. GCF sampling: In group A ,the site with deepest probing depth was selected for GCF sampling while for group B , any site was sampled using PerioPaper strips . Phase I periodontal treatment After baseline GCF sample collection and clinical measurement recordings, group A patients received phase I periodontal treatment. Analysis of Il-17 and Il-18 levels in GCF: GCF samples were examined by enzyme-linked immunosorbent assay (ELISA).Procedures were performed according to the directions in the kits.

Official Title
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Effect of Non Surgical Periodontal Treatment on Clinical Parameters and Gingival Crevicular Fluid Il-17 and Il-18 Levels in Aggressive Periodontitis Patients

Conditions
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Aggressive Periodontitis

Intervention / Treatment
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* Procedure: non surgical periodontal therapy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: -Medically free subjects. Exclusion Criteria: Subjects with periodontal surgeries in the last 6 months. Subjects with prior use of antibiotics in the last 6 months. Smokers. Pregnant females. Lactating females.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Aggressive periodontitis<br>25 young medically free patients with deep periodontal pockets >5mm | Procedure: non surgical periodontal therapy<br>* Scaling and root planing<br>|
| Periodontally healthy individuals<br>25 young medically free individuals with no periodontal inflammation signs | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Periopaper strips Il-17 and Il-18 GCF measurements | measurement of Il-17 and Il-18 in GCF from aggressive periodontitis patients using periopaper strips | Change from Baseline Il-17 and Il-18 at 3 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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gingival crevicular fluid