Active Matrix Metalloproteinase-8 (aMMP-8) Chair-side Test in Different Periodontal Disease

Study Overview
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Brief Summary
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The aim of this study to evaluate the diagnostic accuracy of a new qualitative aMMP-8 POC test in different periodontal health and disease and as compared to the established quantitative laboratory method (ELISA)

Detailed Description
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MMP-8 has been reported to reflect the inflammatory status in periodontal tissues and, therefore represent as potential biomarkers of periodontitis. A positive aMMP-8 test could be an early indicator of these excessive and undesired tissue reactions.

Official Title
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Evaluation of Active Matrix Metalloproteinase-8 (aMMP-8) Chair-side Test in Different Periodontal Disease and Healthy Implants

Conditions
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Periodontal Diseases

Intervention / Treatment
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* Diagnostic Test: GCF collection with aMMP-8 chair side test
* Other: Saliva collection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Systemically healthy Nonsmokers Exclusion Criteria: Participants were not included if they had systemic disease such as diabetes, cardiovascular. Participants who took regular medications that could affect of periodontal tissue Participants who received antibiotics less than 10 weeks before the study

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Chronic periodontitis<br>This groups participant has radiographically moderate alveolar bone loss, CAL > 5 mm and PD >6 mm in several sites of each quadrant | Diagnostic Test: GCF collection with aMMP-8 chair side test<br> <br> Other: Saliva collection<br> <br> |
| Generalized aggressive periodontitis<br>This demonstrated a generalized pattern of severe breakdown and CAL > 5 mm and PD > 6 mm on 8 > teeth; minimum three of those were other than first incisors or first molars | Diagnostic Test: GCF collection with aMMP-8 chair side test<br> <br> Other: Saliva collection<br> <br> |
| Gingivitis<br>This group has varying degrees of gingival inflammation, with CAL < 2 mm, without any radiographical bone loss due to periodontitis | Diagnostic Test: GCF collection with aMMP-8 chair side test<br> <br> Other: Saliva collection<br> <br> |
| Implant<br>Implants classified PD < 5 mm, no bleeding on probing, no suppuration and no radiographic bone loss > 0.5 mm | Diagnostic Test: GCF collection with aMMP-8 chair side test<br> <br> Other: Saliva collection<br> <br> |
| Health<br>Probing depth (PD) < 3mm, no gingival recession due to periodontal disease, and clinical attachment level (CAL) < 2 mm, BOP in < 10% of full-mouth score examination | Diagnostic Test: GCF collection with aMMP-8 chair side test<br> <br> Other: Saliva collection<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| aMMP-8 levels of GCF | GCF levels measured by IFMA | 2 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Salivary MMP-8 levels | Saliva levels measured by IFMA | 2 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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aggressive periodontitis, healthy peri-implant, chairside test, chronic periodontitis