Impact of Platinum-based Cancer Treatment on Endothelial Function

Study Overview
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Brief Summary
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In this research we investigate endothelial function in cancer patients who received platinum based chemotherapy.

Detailed Description
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The goal of the study is to evaluate endothelial function in patients with platinum-based versus non-platinum-based chemotherapy. Endothelial function was evaluated via Angioscan( the apparatus that evaluate the stiffness of vessels by pulse wave velocity) and serum levels of endothelial nitric oxide synthase (ENOS),endothelin1. Patients with gastrointestinal, ovarian, bladder and brest cancer would be included. Patients with malignancies were followed-up from 2 weeks to 3 month after start of chemotherapy, Medical records and hospital databases were searched for all the patients treated with different chemotherapy treatment for any kind of cancer.

Official Title
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The Effect of Polychemotherapy on Endothelial Function Ans Vessel Age in Cancer Patients.

Conditions
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High Blood Pressure, Cardiovascular Complication

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Subject has solid cancer Subject is expected to start chemotherapy Exclusion Criteria: Pregnancy and lactation Severe impaired kidney function Severe hepatic impairment Mental disease Acute myocardial infarction 28 days ago or earlier Acute Cerebrovascular Event 1 month ago or earlier Rhythm disturbance that complicate pulse wave velocity evaluation

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Evaluation of blood pressure | Both systolic and diastiolic blood pressure were measured in the office in the seated position, noninvasively. | Before start of chemotherapy (1 hour after including in the trial) | 
| Evaluation of flow-mediated vasodilation and pulse-wave velocity | Flow-mediated dilatation and pulse-wave velocity were measured via Angioscan apparatus in the seated position.The cuff inflation period of 5 min was initially decided to produce adequate hyperaemia to allow flow-mediated dilatation. The scanning period used in our trial was 1 minite before and 9 minutes after the cuff deflation. | Before start of chemotherapy (1 hour after including in the trial) | 
| Evaluation of concetration of ENOS and Big Endothelin in blood sampling | Blood was collected from the cubital vein to 10.0 ml/ 6.0 ml red top tubes (without clot activator). The sample was then separated by centrifugation. The serum was frozen to -20C. | Before start of chemotherapy (1 hour after including in the trial) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Evaluation of blood pressure | Both systolic and diastiolic blood pressure was measured in the office in the seated position, noninvasively. | In range of 3 -12 weeks after start of chemotherapy (before next chepotherapy drug injection) | 
| Evaluation of flow-mediated vasodilation and pulse-wave velocity | Flow-mediated dilatation and pulse-wave velocity were measured via Angioscan apparatus in the seated position.The cuff inflation period of 5 min was initially decided to produce adequate hyperaemia to allow flow-mediated dilatation. The scanning period used in our trial was 1 minite before and 9 minutes after the cuff deflation. | In range of 3 -12 weeks after start of chemotherapy (before next chepotherapy drug injection) | 
| Evaluation of concetration of ENOS and Big Endothelin of blood sampling | Blood was collected from the cubital vein to 10.0 ml/ 6.0 ml red top tubes (without clot activator). The sample was then separated by centrifugation. The serum was frozen to -20C. | In range of 3 -12 weeks after start of chemotherapy (before next chepotherapy drug injection) |