New Method Using Deuterium Labelled Creatine to Measure Total Body Skeletal Muscle Mass

Study Overview
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Brief Summary
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Creatine (methyl-d3) dilution (D3-creatine) is a novel technique for the estimation of muscle mass. The method uses a dose of deuterium-labelled creatine to determine total skeletal muscle mass via estimation of total body creatine pool size.The aim of this study is to compare estimates of total body skeletal muscle mass by D3-creatine dilution method and whole body Magnetic Resonance Imaging (MRI) in an athletic population.

Official Title
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Total Body Skeletal Muscle Mass Estimation by Creatine (Methyl-d3) Dilution in Athletes

Conditions
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Sports Nutritional Sciences

Intervention / Treatment
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* Dietary Supplement: D3 Creatine
* Dietary Supplement: Creatine


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Trained and competing elite canoe athletes will be recruited, who represent Great Britain Canoeing Exclusion Criteria: Pregnant and lactating women Intolerance or hypersensitivity to Creatine supplementation Athletes with disabilities which affect their total body muscle mass If creatine was taken in a supplemented form, rather than dietary intake, more regularly than once a week within 6-weeks of assessment commencing Contraindication to an MRI scan

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: D3 Creatine followed by creatine supplementation<br>Every participant will be orally administered with 60mg of D3 Creatine in a fasted state in a non-gelatin capsule. Creatine supplemented throughout the study will be administered in the following doses-0.03, 0.035, 0.04, 0.045 g.kg.Lean mass/day, increasing on weekly basis. Final dose of 0.05 g.kg.Lean mass/day is then maintained throughout the study. | Dietary Supplement: D3 Creatine<br>* One dose of D3 Creatine (60mg capsule) given orally on Day 1<br>Dietary Supplement: Creatine<br>* Participants will consume an individual dosed level of creatine, based on their estimated muscle mass in doses of 0.03, 0.035, 0.04, 0.045 g.kg.Lean mass/day, increasing on a weekly basis. Final dose of 0.05 g.kg.Lean mass/day is then maintained throughout the study.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Correlation between muscle mass obtained by MRI method and D3-Creatine method | MRI scan of the participants will be done at Day 1 and end of study (i.e.Day 90).D3-Creatine capsule will be given orally at Day 1 and urine samples collected at 0-4h and 4-24h every day from Day 1 to Day 4. | Upto 3 months |