Protein in Feeding Strategies to Accelerate Muscle Recovery From ACL Reconstruction

Study Overview
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Brief Summary
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Musculoskeletal injury, particularly injuries requiring surgical repair, induce significant muscle atrophy, resulting in diminished physical function. The protein turnover-associated (diminished protein synthesis, elevated protein breakdown, negative net muscle protein balance) etiology of injury and surgical-induced atrophy are, in part, similar to those observed with muscle wasting induced by hyper-catabolic and inflammatory conditions and prolonged periods of skeletal muscle disuse (e.g., cachexia and joint immobilization). Combining dietary strategies to optimize the anabolic properties of beef as a high quality protein source that provides essential amino acids prior to surgery and throughout post-surgery rehabilitation may attenuate muscle atrophy and accelerate the restoration of muscle function. This project will examine the efficacy of habitual consumption of a beef-based higher protein meal pattern and post-physical rehabilitation beef protein supplementation throughout post-surgical rehabilitation intervention on skeletal muscle function in response to anterior cruciate ligament (ACL) repair in healthy, physically active adults. Stable isotope methodologies, proteomic analysis of muscle protein synthesis, and molecular assessments of skeletal muscle atrophy and remodeling will be assessed to evaluate the effects of increased protein intake, including consumption of a high quality beef-based protein supplement following patient physical therapy sessions on: Skeletal muscle protein fractional synthetic rates during and in recovery from surgery; Inflammatory, proteolytic, and anabolic intramuscular signaling during surgery and their association with skeletal muscle protein synthesis and muscle function; Muscle size and functional recovery; Duration to return to routine exercise, sport, or physical activity.

Official Title
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The Role of Beef in Optimal Protein and Feeding Strategies to Accelerate Muscle Mass and Functional Recovery From Anterior Cruciate Ligament (ACL) Repair

Conditions
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Rehabilitative Nutrition for Muscle Recovery From ACL Reconstruction

Intervention / Treatment
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* Other: Diet Intervention_AP-CPT
* Other: Diet Intervention_PP-PPT


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: 18 to 50 years of age identified as appropriate for study recruitment by their respective orthopedic surgeon undergoing their first anterior cruciate ligament (ACL) repair undergo ACL reconstruction under general anesthesia body mass indexes (BMIs) within normal to overweight ranges Exclusion Criteria: reporting metabolic or cardiovascular abnormalities, food allergies, gastrointestinal disorders (i.e. lactose intolerance) use of nutritional/herbal/sports supplements including anabolic steroids, and tobacco products will be excluded from the study BMIs above 29.9

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 50 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Adequate Protein_Carbohydrate Post Therapy<br>Diet Intervention - Adequate Protein (1.0 g/kg/d) - Carbohydrate Post Therapy [AP-CPT] | Other: Diet Intervention_AP-CPT<br>* Adequate Protein_Carbohydrate Post Therapy (AP-CPT) where protein intake will be 1 g/kg/d for the duration of the study .<br>|
| Experimental: Optimal Protein_Protein Post Therapy<br>Diet Intervention - Optimal Protein (2.0 g/kg/d) - Protein Post Therapy [OP-PPT] | Other: Diet Intervention_PP-PPT<br>* Optimal Protein_Protein Post Therapy (OP-PPT) with protein intake set at 2.0 g/kg/day for the duration of the study<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Skeletal muscle protein fractional synthetic rates | Using stable isotope techniques skeletal muscle protein fractional synthetic rates will be determined | Following 16 weeks of physical rehabilitation post surgery for subjects in both diet intervention groups. | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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rehabilitative nutrition, protein, beef, ACL reconstruction, protein synthesis, proteolysis, muscle mass, muscle strength