Inflammatory Cytokines in Congenital Cataract After Lensectomy and Anterior Vitrectomy

Study Overview
=================
Brief Summary
-----------------
In this prospective study , a cohort of 58 eyes with congenital cataract from Wenzhou medical university was erolled.33 eyes with congenital cataract was after lensectomy and anterior vitrectomy as post-surger group, 26 eyes without cataract surgery as control group. The cytokines of MCP-1, VEGF, IL-1, IFN-γ, IL-1r,IL-4,IL-17, IL-2,IL-5,G-CSF,IL-12, IL-15 was evaluated

Detailed Description
-----------------
Secondary IOL implantation in aphika children and lensectomy and anterior vitrectomy and primary IOL implantation in children with congenital cataract were both performed by the same surgeon(Z.Y.E) and videos were collected to review the capsular reconstruction condition and lens implantation position. Aqueous humor (100-200 μl) was aspirated from each patient before the surgery. The research was to evaluate the relationship between the cytokine profile in aqueous humor and the state of capsule after Lensectomy and Anterior Vitrectomy.Aqueous humor was obtained , Human Magnetic Luminex Screening Assay was used to determine the aqueous humor levels of cytokines.

Official Title
-----------------
Expression Profile of Inflammatory Cytokines in Congenital Cataract After Lensectomy and Anterior Vitrectomy

Conditions
-----------------
Congenital Cataract

Intervention / Treatment
-----------------
* Procedure: secondary IOL implantation
* Procedure: primary IOL implantation


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: aphakic children diagnosed with congenital cataract who underwent secondary IOL implantation and children with congenital cataract who underwent lensectomy and anterior vitrectomy and primary IOL implantation Exclusion Criteria:patients with mild cataracts do not require surgery -

Ages Eligible for Study
-----------------
Minimum Age: 10 Months
Maximum Age: 72 Months

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
No

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| SIOL group<br>aphakic children diagnosed with congenital cataract who underwent secondary IOL implantation | Procedure: secondary IOL implantation<br>* reopened the fibrotic, closed capsular bag and achieve IOL placement<br>|
| PIOL group<br>children with congenital cataract who underwent lensectomy and anterior vitrectomy and primary IOL implantation | Procedure: primary IOL implantation<br>* lensectomy and anterior vitrectomy and primary IOL implantation<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| inflammatory cytokines | investigate inflammatory microenvironment changes in the aqueous of children after surgery.aqueous humor levels of cytokines, chemokines and growth factors including TGFβ2, TNF-α, IL-6, IP10, IL-10, MCP-1, VEGF, IL-1, IFN-γ, IL-1r,IL-4,IL-17, IL-2,IL-5,G-CSF,IL-12, IL-15. | before the surgery of secondary IOL implantation | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
inflammatory cytokines