Outcomes of the Embryos of Different Ovarian Follicle Sizes, and the miRNA Detection in Their Blastocyst Culture

Study Overview
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Brief Summary
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In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.

Detailed Description
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In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.

Official Title
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Evaluation of the Outcomes of the Embryos Obtained From Different Follicle Sizes, and the miRNA Detection in the Spent Media of Their Blastocyst Culture

Conditions
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Embryo Quality

Intervention / Treatment
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* Procedure: Oocyte pick-up


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Female patients aged between 20-38 who are planned to have IVF treatment On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm Patient having BMI below 30 Exclusion Criteria: PCO patients Patients having endometriosis Patients having male factor with severe oligoazoospermia

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 38 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Women referring to infertility clinic for IVF treatment<br>Women who were referred to infertility clinic with complaint of not having baby go through IVF treatment, and having oocyte pick-up procedure with different sizes of ovarian follicles | Procedure: Oocyte pick-up<br>* With the needle, transvaginally USG-guided oocyte pick-up procedure is going to be performed<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oocyte grading | Morphologic Oocyte grading will be analyzed right after the procedure due to different follicle sizes | after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started | 
| Day-3 Embryo Grading | Morphologic Day-3 embryo grading will be analyzed after the procedure due to different follicle sizes | Three days after the intracytoplasminc sperm injection(ICSI) procedure | 
| Day-5 Embryo Grading | Day-5 embryo grading(Gardner classification) will be analyzed after the procedure due to different follicle sizes | 5 days after the intracytoplasminc sperm injection(ICSI) procedure | 
| beta hCG results of the transferred embryos | Clinical pregnancy outcomes | ten days after the embryo transfer | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| micro RNA expression in culture medium | In the culture medium of blastocysts are going to be analyzed for microRNA levels that are expressed | Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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follicle size, micro RNA, blastocyst culture, embryo quality