Proteoglycan 4 Mechanism the Effectiveness of Pulmaonry Recovery in COPD Patients

Study Overview
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Brief Summary
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The investigators previous research has demonstrated that proteoglycan 4 (PRG4) may be a biomarker for identification of severity in COPD. PRG4 was more sensitive and specific than CRP for confirming COPD severity and acute exacerbation frequency. It was related to the 1-year force vital capacity decline in COPD patients. The past study found that Prg4 is an immunomodulatory factor regulating parathyroid hormone actions on hematopoietic cells in mice. Previous report showed that voluntary wheel running and fluid flow shear stress that promote the expression of the Prg4 and association with pulmonary inflammation. COPD patients are characterized by a progressive decrease of lung function that is associated with increased in the airway and systemic inflammation. Pulmonary recovery (PR) is able to decrease acute exacerbation, maintain pulmonary function, increase exercise tolerance and improve quality of life in COPD patients, but it is unknown the mechanism of PRG4. The current study aimed to study in the pulmonary inflammation and the effectiveness of pulmonary recovery in COPD Patients：The mechanism of PRG 4.

Detailed Description
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The investigators previous research has demonstrated that proteoglycan 4 (PRG4) may be a biomarker for identification of severity in COPD. PRG4 was more sensitive and specific than CRP for confirming COPD severity and acute exacerbation frequency. It was related to the 1-year force vital capacity decline in COPD patients. The past study found that Prg4 is an immunomodulatory factor regulating parathyroid hormone actions on hematopoietic cells in mice. Previous report showed that voluntary wheel running and fluid flow shear stress that promote the expression of the Prg4 and association with pulmonary inflammation. COPD patients are characterized by a progressive decrease of lung function that is associated with increased in the airway and systemic inflammation. Pulmonary recovery (PR) is able to decrease acute exacerbation, maintain pulmonary function, increase exercise tolerance and improve quality of life in COPD patients, but it is unknown the mechanism of PRG4. The current study aimed to study in the pulmonary inflammation and the effectiveness of pulmonary recovery in COPD Patients：The mechanism of PRG 4. Proteoglycan 4 is correlated with pulmonary inflammation and decline of pulmonary function in the different phenotype of COPD. 1-1 Classification of characteristic、the severity of disease、pulmonary functional test、exercise capacity and blood sample collection for routine analysis. 1-2 Human blood bio-bank and molecular analysis: PRG4、IL-6、IL-8、TNFα、CRP& IL-10. 1-3 To investigate whether daily physical activity、hypoxia and hyperinflation were associated with airway inflammation and PRG4 in COPD patients. The mechanism of PRG 4 in the effectiveness of PR on the AECOPD. 2-1 The PR program: The combination effect of negative pressure ventilation and exercise training program on acute exacerbation of COPD (AECOPD). 2-2 To examine the efficacy of PR on cytokines and PRG4 protein. 2-3 Correlation between PR effects and PRG4：PR is correlation with PRG4、clinical progression、exercise tolerance、PFT change and SpO2 during exercise. In vitro cell culture research models 3-1 Pulmonary inflammatory model establishment：Human primary epithelium cell culture and Beas-2B for IL-1β、TNFα、LPS、IL-10 and H2O2 effects. 3-2 Purification of peripheral blood monocyte cells：Primary culture to evaluate pulmonary inflammation and treatment effects. 3-3 Hypoxia experiments：To test whether hypoxia induces regulation of PRG4 protein or different cytokines in epithelial cells. 3-4 Inhibit PRG4：To prove the mechanism of PRG4 under the hypoxia model by PRG4 siRNA。 In vivo animal research models 4-1 Smoke-COPD mice models establishment： 4-2 To determinate the effects of exercise training in COPD mine. 4-3 To investigate on PRG4 protein in pulmonary inflammation and the effectiveness of exercise training in the tissue and serum of mice.

Official Title
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Studies in Pulmonary Inflammation and the Effectiveness of Pulmonary Recovery in COPD Patients：the Mechanism of Proteoglycan 4

Conditions
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COPD

Intervention / Treatment
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* Device: Pulmonary recovery on the AECOPD
* Other: Routine care on the AECOPD
* Other: Routine care on the stable COPD


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Hospitalized patients with acute exacerbation of COPD GOLD diagnostic criteria based on the COPD, the use of inhaled bronchodilators: the first second forced expiratory volume (FEV1.0) / forced vital capacity (FVC) <70%. Age ≧ 40 and ≦ 80 years old, is currently not accepting any research project. Physician assess the patient is currently in stable condition vital signs, complain dyspnea of exercise, consult RT for pulmonary rehabilitation. The purpose of this study by researchers explain, the researchers agreed to participate in this. Stable COPD outpatients GOLD diagnostic criteria based on the COPD, the use of inhaled bronchodilators: the first second forced expiratory volume (FEV1.0) / forced vital capacity (FVC) <70%. Age ≧ 40 and ≦ 80 years old, is currently not accepting any research project. The purpose of this study by researchers explain, the researchers agreed to participate in this. Exclusion Criteria: Hospitalized patients with acute exacerbation of COPD Unstable vital signs: blood pressure <90 mm Hg, blood oxygen levels (SpO2) at Tx under still <85%. Associated with neuromuscular function disorders such as hemiplegia or no independent walking function. Have been diagnosed with severe mental disorders, such as dementia or or unable to cooperating people. Stable COPD outpatients Over the past three months there have been acute infection or are in a state of acute attacks. Associated with neuromuscular function disorders such as hemiplegia or no independent walking function. Have been diagnosed with severe mental disorders, such as dementia or unable to cooperating people

Ages Eligible for Study
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Minimum Age: 40 Years
Maximum Age: 85 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Health Services Research
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Pulmonary recovery on the AECOPD<br>Study in the pulmonary inflammation and the effectiveness of pulmonary recovery in AECOPD Patients | Device: Pulmonary recovery on the AECOPD<br>* The combination effect of negative pressure ventilation, limb muscle exercise training program and fibit monitor on acute exacerbation of AECOPD. To investigate the daily physical activity in routine care patients with after AECOPD and stable stage.<br>* Other names: Fibit monitor;Other: Routine care on the AECOPD<br>* To investigate whether daily physical activity、hypoxia and hyperinflation were associated with airway inflammation and PRG4 in Routine care patients wtih AECOPD and stable stage.<br>* Other names: Fibit monitor;Other: Routine care on the stable COPD<br>* To investigate whether daily physical activity、hypoxia and hyperinflation were associated with airway inflammation and PRG4 in Non-Pulmanary recovery patients wtih stable COPD.<br>* Other names: Fibit monitor;|
| Other: Routine care on the AECOPD<br>Study in the pulmonary inflammation and the effectiveness of pulmonary recovery in AECOPD Patients | Other: Routine care on the AECOPD<br>* To investigate whether daily physical activity、hypoxia and hyperinflation were associated with airway inflammation and PRG4 in Routine care patients wtih AECOPD and stable stage.<br>* Other names: Fibit monitor;Other: Routine care on the stable COPD<br>* To investigate whether daily physical activity、hypoxia and hyperinflation were associated with airway inflammation and PRG4 in Non-Pulmanary recovery patients wtih stable COPD.<br>* Other names: Fibit monitor;|
| Other: Routine care on the stable COPD<br>Study in the pulmonary inflammation and the effectiveness of pulmonary recovery in stabel COPD Patients | Other: Routine care on the stable COPD<br>* To investigate whether daily physical activity、hypoxia and hyperinflation were associated with airway inflammation and PRG4 in Non-Pulmanary recovery patients wtih stable COPD.<br>* Other names: Fibit monitor;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The systemic inflamation biomakers | Measured the proteaglycan4、IL-6、IL-8、TNFα、CRP& IL-10 from the supernatant with COPD patients | 0 12 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Exercise capacity | Six-minute walking distance and lung | 0 12 months | 
| The correlaton with the exercise capacity and systemic inflammationcytokines | volumePulmonary recovery is correlation with PRG4、clinical progression、exercise tolerance、PFT change and SpO2 during exercise | 0 12 months |