Serum Proteomics Analysis for Sepsis

Study Overview
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Brief Summary
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Serum proteomics is a very useful tool to identify various disease. The purpose of the present study was to find differential proteins among patient with normal, SIRS, sepsis, severe sepsis, death and to screen potential biomarkers for their dynamic changes. Serum proteins were identified by iTRAQ labeling and LC-MS/MS. The bioinformatics analysis was performed with the Mascot software and the International Protein Index (IPI) and the Gene Ontology (GO) Database and KEGG pathway Database. The differentially expressed proteins were verified by Western blot by another sample collected from clinical.

Official Title
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Serum Proteomics Analysis for Dynamic Changes of Differential Proteins in Sepsis Patient With iTRAQ Labeling and LC-MS/MS

Conditions
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SIRS, Sepsis, Severe Sepsis, Death

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male and female aged 18 years old and over; clinically confirmed infection; fulfilled at least two criteria of systemic inflammatory response syndrome (a) core temperature higher than 38 °C or lower than 36 °C (b)respiratory rate above 20/min, or PCO2 below 32 mmHg (c) pulse rate above 90/min, and (d) white blood cell count greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands. Exclusion Criteria: younger than 18 years of age; acquired immunodeficiency syndrome; reduced polymorphonuclear granulocyte counts (< 500 μL-1); died within 24h after admission into the ICU, or refused to participate in the study, or declined treatment during the period of observation.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Normal<br>normal person under physical examination | |
| SIRS<br>temperature >38 ℃ or <36℃; pulse rate>90 beats/min; ventilatory rate>20 breaths/min or hyperventilation with partial pressure of arterial carbon dioxide (PaCO2)<32mmHg; white blood cell count>12,000μL-1 or <4000μL-1 or >10% immature cells | |
| spesis<br>sepsis is defined as SIRS plus confirmed infection. | |
| severe sepsis<br>severe sepsis: sepsis associated with organ dysfunction, hypoperfusion, or hypotension; septic shock: sepsis with arterial hypotension, despite adequate ﬂuid resuscitation. | |
| death<br>sepsis patients within 48 hours before death | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Survival status | The survival time of patients more than 28days is defined as survival. The survival time of patients less than 28days is defined as death. | 28 days after admission to ICU | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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proteomics,, serum,, sepsis,, potential biomarkers,, dynamic changes, normal