Expression of the Inhibitory Receptors on Lymphocytes T Cells After Lung Cancer Surgery.

Study Overview
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Brief Summary
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Postoperative pneumonia is one of the most common complications after lung cancer surgery and associated with a morbidity and mortality. Postoperative lymphopenia has been recently identified as one of risk factors for postoperative pneumonia. According to recent studies in polytrauma, cancer or septic shock, T cells dysfunction may be related to high expression of inhibitory receptors on lymphocytes.

Detailed Description
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This study will investigate prospectively TIM-3, PD-1 and CTLA4 expression on lymphocytes T cells before and after lung cancer surgery. Patients ≥ 18 years with no history of immunosuppressive state will be included.

Official Title
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Expression of the Inhibitory Receptors on Lymphocytes T Cells After Lung Cancer Surgery.

Conditions
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Lung Cancer, Surgery

Intervention / Treatment
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* Biological: Blood samples


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient ≥ 18 years undergoing lung cancer surgery by thoracoscopy Exclusion Criteria: Patient under guardianship/curatorship Patient who received of radiotherapy or chemotherapy in the last 6 months Patient under immunosuppressive treatment or dose of corticoids over 10 mg/day prednisolone or equivalent Patient with history of malignant blood disease or auto-immune disease Patient suffering from HIV infection and Patients with pre-operative infection Patient with an empiric antibiotic therapy introduced at operating room

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Patients undergoing lung cancer surgery<br>Patients undergoing lung cancer surgery will be included. Blood samples will be collected. | Biological: Blood samples<br>* Blood samples will be collected in patients undergoing lung cancer surgery: before lung cancer surgery, the next day lung cancer surgery, 3 days after lung cancer surgery. They will be treated in flow cytometry.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| PD-1, TIM-3, CTLA4 on CD4/CD8 lymphocytes | Correlation between expression of PD-1, TIM-3, CTLA4 on CD4/CD8 lymphocytes and the occurrence of lymphopenia. Measured by blood sample before lung cancer surgery, the next day lung cancer surgery and 3 days after lung cancer surgery. Analyzed by multicolour immunolabelling in flow cytometry | Days 0, 1, 3 | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| functionality of CD4/CD8 lymphocytes and production of IF gamma, TNF and IL-2 | Correlation between functionality of CD4/CD8 lymphocytes and production of interferon (IF) gamma, Tumor Necrosis Factor (TNF) and interleukine (IL-2). Measured by blood sample before lung cancer surgery, the next day lung cancer surgery and 3 days after lung cancer surgery. Analyzed by multicolour immunolabelling in flow cytometry | Days 0, 1, 3 | 
| pneumonia | To analyse the occurrence of postoperative pneumonia. | Days 3 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Lymphopenia, Lung cancer surgery, TIM-3, PD-1, CTLA-4, lymphocytes, CD4, CD8