The Effect of Degree of Rice-polishing on Glycaemic Response

Study Overview
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Brief Summary
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To investigate the effects of rice polishing on glycaemic and insulinaemic response in healthy Chinese male volunteers.

Detailed Description
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There will be a total of 8 test session. Each session will last for 3.5 hours. At each test session, volunteers will be served with test meal and blood will be collected. After an overnight fast, subjects will visit the research center. Two fasting capillary blood samples will be collected via fingerprick. Following the fasting samples, subject will consume the test meals. Further blood samples will be collected at 15, 30, 45, 60, 90, 120, 150, 180 minutes after the ingestion of test meal. These samples will be used to analyses for whole capillary blood glucose. At every 30 minute interval, samples will be collected for analysis of capillary plasma insulin.

Official Title
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The Effect of Degree of Rice-polishing on Glycaemic Response

Conditions
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Diabetes Mellitus, Obesity

Intervention / Treatment
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* Other: Glucose Reference 1
* Other: Glucose Reference 2
* Other: Glucose Reference 3
* Other: Brown Rice
* Other: 3 % polished rice
* Other: 6 % polished rice
* Other: 9 % polished rice
* Other: 20 % Polished rice


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male Chinese ethnicity Age ≥21 and ≤ 60 years Body mass index between 18.5 to 25 kg/m2 Normal blood pressure =<140/90 mmHg Fasting blood glucose <6.0 mmol/L In general good health Exclusion Criteria: Current smoker Have any metabolic diseases (such as diabetes, hypertension etc) Do not have known glucose-6-phosphate dehydrogenase deficiency (G6PD deficiency) Have medical conditions and/or taking medications known to affect glycaemia (glucocorticoids, thyroid hormones, thiazide diuretics) Have intolerances or allergies to any foods Currently partake in sports at the competitive and/or endurance levels Intentionally restrict food intake

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: Randomized crossover design
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: Glucose Reference 1<br>Glucose solution 1 | Other: Glucose Reference 1<br>* 50 g of glucose dissolved in 250 ml of water (First glucose control visit)<br>|
| Other: Glucose Reference 2<br>Glucose solution 2 | Other: Glucose Reference 2<br>* 50 g of glucose dissolved in 250 ml of water (Second glucose control visit)<br>|
| Other: Glucose Reference 3<br>Glucose solution 3 | Other: Glucose Reference 3<br>* 50 g of glucose dissolved in 250 ml of water (Third glucose control visit)<br>|
| Experimental: Brown Rice<br>Cooked brown rice | Other: Brown Rice<br>* Unpolished brown rice containing 50 g of available carbohydrate was cooked using a rice cooker (Toyomi) at a ratio of rice to water of 1: 2<br>|
| Experimental: 3 % polished rice<br>Cooked 3 % polished rice | Other: 3 % polished rice<br>* 3% polished rice, containing 50 g of available carbohydrate was cooked using a rice cooker at a ratio of rice to water of 1: 2<br>|
| Experimental: 6 % polished rice<br>Cooked 6 % polished rice | Other: 6 % polished rice<br>* 6% polished rice, containing 50 g of available carbohydrate was cooked using a rice cooker at a ratio of rice to water of 1: 2<br>|
| Experimental: 9 % polished rice<br>9 % polished rice | Other: 9 % polished rice<br>* 9 % polished rice, containing 50 g of available carbohydrate was cooked using a rice cooker at a ratio of rice to water of 1: 2<br>|
| Experimental: 20 % Polished rice<br>Cooked 20 % Polished rice (White Rice) | Other: 20 % Polished rice<br>* White rice (20% polished rice), containing 50 g of available carbohydrate was cooked using a rice cooker at a ratio of rice to water of 1: 2.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Glucose | Whole capillary blood glucose will be measure using Hemocue analyser | Up to 180 minutes | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Insulin | Capillary plasma will be measure using Cobas analyser | Up to 180 minutes |