Endocan, VEGF and TNF-alpha Levels in Periodontal Disease and After Treatment

Study Overview
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Brief Summary
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The primer aim of the study was to determine serum and gingival crevicular fluid (GCF) endocan levels in periodontal diseases pathogenesis supported with vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α) levels.

Detailed Description
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The study consist of three groups: periodontally healthy individuals (group 1; n = 20), individuals with generalized chronic periodontitis (group 2; n = 20), the same patients with chronic periodontitis after they had been treated with scaling and root planing (SRP) were considered as Group 3. Clinical measurements were recorded; GCF and serum samples were obtained from each participant before and 6 weeks after therapy. Endocan, VEGF-A and TNF-α levels were measured by enzyme-linked immunosorbent assay.

Official Title
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Effect of Non-surgical Periodontal Treatment on Gingival Crevicular Fluid and Serum Endocan, Vascular Endothelial Growth Factor-A and Tumor Necrosis Factor Alpha Levels

Conditions
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Periodontal Disease

Intervention / Treatment
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* Procedure: Non-surgical periodontal treatment


Participation Criteria
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Eligibility Criteria
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Group 1 Inclusion Criteria: No bone and attachment loss, GI=0, PPD≤3mm, CAL≤3mm Systematically healthy patients Group 2 Inclusion Criteria: GI>1, PPD≥5mm, CAL≥5mm with alveolar bone loss radiographically. Systematically healthy patients Exclusion Criteria: Aggressive Periodontitis, Oral pathologies, Patients with any other systemic diseases, Pregnant women and those in the lactation period, Patients with smoking habit and taking medication Patients received periodontal therapy in last 6 months

Ages Eligible for Study
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Minimum Age: 25 Years
Maximum Age: 49 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Group 1<br>Periodontal healthy individuals | |
| Group 2<br>Patients with chronic periodontitis | Procedure: Non-surgical periodontal treatment<br>* Non-surgical periodontal therapy is an anti-infective therapy which includes both mechanical and chemotherapeutic approaches to minimize or eliminate microbial biofilm, the primary etiology of gingivitis and periodontitis<br>|
| Group 3<br>the same patients in group 2 after they had been treated with scaling and root planing (SRP) were considered as Group 3. | Procedure: Non-surgical periodontal treatment<br>* Non-surgical periodontal therapy is an anti-infective therapy which includes both mechanical and chemotherapeutic approaches to minimize or eliminate microbial biofilm, the primary etiology of gingivitis and periodontitis<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Endocan levels | Change after periodontal treatment | 6 weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| VEGF levels | Change after periodontal treatment | 6 weeks | 
| TNF-alpha levels | Change after periodontal treatment | 6 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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endocan protein, VEGF A, TNF-alpha, after treatment