Pilot Study: The Effect of Ergocalciferol on Plasma Mucin-1 Levels

Study Overview
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Brief Summary
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To determine if a one-time oral administration of ergocalciferol results in a decline in plasma mucin-1 levels in healthy individuals and individuals with ADTKD-MUC1.

Detailed Description
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This is a pilot, open-labelled, to determine if oral administration of a single dose of ergocalciferol lowers plasma mucin-1 levels. The study population will include 40 healthy individuals and 40 individuals with ADTKD-MUC1. The study consists of an initial screening, signing of consent of interested individuals, performance of pregnancy test in women of child-bearing age and screening labs, and administration of 200,000 units of ergocalciferol on Day 0 (On Study). Laboratory studies will be performed at baseline, on Days 3, 7, and 10.

Official Title
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Study of the Effect of Ergocalciferol on Plasma and Urinary Mucin-1 Levels in Healthy Individuals and Individuals With Autosomal Dominant Tubulo-Interstitial Kidney Disease Due to MUC1 Mutations (ADTKD-MUC1)

Conditions
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Ergocalciferol

Intervention / Treatment
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* Drug: Ergocalciferol


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age > 18 years and <65 years Females must be non-pregnant, non-lactating and fulfilling one of the following: a. Post menopausal defined as amenorrhea for at least 12 months following cessation of all exogenous hormonal treatments. b. Status post irreversible surgical sterilization by hysterectomy, bilateral oophorectomy or bilateral salpingectomy but not tubal ligation. c. Use of acceptable contraceptive method: IUD with spermicide, a female condom with spermicide, contraceptive sponge with spermicide, an intravaginal system(e.g., NuvaRing®), a diaphragm with spermicide, a cervical cap with spermicide, or oral, implantable, transdermal, or injectable contraceptives, sexual abstinence, or a sterile sexual partner. Males must agree to avoid fathering a child (or donating sperm), and therefore be either sterile or agree to use, from the time of enrollment until 45 days after end of study, one of the following approved methods of contraception: a male condom with spermicide, a sterile sexual partner, use by female sexual partner of an IUD with spermicide, a female condom with spermicide, contraceptive sponge with spermicide, an intravaginal system (e.g.,NuvaRing®), a diaphragm with spermicide, a cervical cap with spermicide, or oral, implantable, transdermal, or injectable contraceptives. No serious health conditions except for estimated glomerular filtration rate <30 ml/min/1.73 m2 (according to the modified MDRD formula) in participants with ADTKD- MUC1. Prior established genetic diagnosis of ADTKD-MUC1 in participants recruited with ADTKD-MUC1. Able to understand and comply with requirements of the entire study and to communicate with the study team. Living in close enough proximity to a local laboratory to obtain labwork for the study. Written informed consent using a document that has been approved by the Institutional Review Board. The participant has not taken ergocalciferol in the month prior to study initiation. If receiving cholecalciferol, the dose is < 1000/day and has been stopped one month prior to Day 0 (On Study). Participants agree NOT to take ergocalciferol or cholecalciferol (except for<500 IU that contained in a daily multi-vitamin) for six months after receiving the one-time dose of ergocalciferol. Exclusion Criteria: Major systemic illness other than chronic kidney disease in individuals with ADTKD- MUC1. A history of granulomatous disorders (sarcoidosis, tuberculosis), hyperparathyroidism, or other disorders of calcium metabolism. A history of nephrolithiasis. A history of osteoporosis or osteopenia. A fracture or a fall that did not occur during exercise within the last six months. Intolerance or known allergic reaction to ergocalciferol. 25 hydroxy Vitamin D level > upper limit of normal. Lactating. Liver disease. Receiving glucocorticoids.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 65 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Healthy Group<br>40 Healthy individuals will receive a single dose of ergocalciferol (200,000 units) | Drug: Ergocalciferol<br>* Investigational drug will be administered to eligible participants in a single dose of 200,000 USP units vitamin D<br>* Other names: Vitamin D2;|
| Experimental: ADTKD-MUC1 Group<br>40 individuals with ADTKD-MUC1 (Autosomal Dominant Tubulo-Interstitial Kidney Disease- a rare disease caused by mutation in MUC1) will receive a single dose of ergocalciferol (200,000 units) | Drug: Ergocalciferol<br>* Investigational drug will be administered to eligible participants in a single dose of 200,000 USP units vitamin D<br>* Other names: Vitamin D2;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma mucin-1 levels | Plasma mucin-1 levels will be measured in both groups. using a two-tailed T-test. | Baseline | 
| Plasma mucin-1 levels | Plasma mucin-1 levels will be measured in both groups. using a two-tailed T-test. | 3 days | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Plasma mucin-1 levels | Plasma mucin-1 levels will be measured in will be measured in both groups. | 7 days | 
| Plasma mucin-1 levels | Plasma mucin-1 levels will be measured in will be measured in both groups. | 10 days | 
| Urinary mucin-1 levels in healthy controls | Urinary mucin-1 levels | Baseline | 
| Urinary mucin-1 levels in healthy controls | Urinary mucin-1 levels | 3 days | 
| Urinary mucin-1 levels in healthy controls | Urinary mucin-1 levels | 7 days | 
| Urinary mucin-1 levels in healthy controls | Urinary mucin-1 levels | 10 days | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Vitamin D Deficiency, Drisdol, Rickets, Plasma, Healthy Subjects, Autosomal Dominant Tubulo Interstitial Kidney Disease