Significance of the High-risk Hpv Viral Load

Study Overview
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Brief Summary
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The aim of this study is to evaluate the significance of an elevated high-risk HPV (HR-HPV) viral load.

Detailed Description
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We will particularly evaluate if the HR-HPV viral load: Is correlated to the transcription of the genes of the oncoproteins E6 and E7 Is predictive of the integration of the virus in infected cells Is predictive of the presence of HPV in patients' blood Influences the local and systemic immunologic response

Official Title
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Significance of the High-risk Hpv Viral Load

Conditions
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HPV Infections

Intervention / Treatment
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* Other: 2 cervical takings and 2 sampling of blood
* Other: 2 cervical takings and 2 sampling of blood
* Other: 2 cervical takings and 2 sampling of blood


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The women consulting for the therapeutic care (by hysterectomy or conization) of a LIEHG or a cervical cancer (carcinoma epidermoid or adenocarcinoma) confirmed by the anatomopathologique analysis of a preoperative cervical biopsy. The women having a normal cervix consulting for the surgical care by hysterectomy of a good-hearted pathology of the womb. The normality of the cervix will be confirmed by the anatomopathologique analysis of the detail of hysterectomy The patients participating in this study will have to be of more than 18 years old. All the subjects will be profitable of a regime of Social Security. Exclusion Criteria: The patients having a LIEBG The patients incapable to receive the information enlightened on the progress and the objectives of the study The patients not having signed enlightened assent

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Other
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: 1<br>125 womens with normal cervix | Other: 2 cervical takings and 2 sampling of blood<br>* 2 cervical takings 2 sampling of blood before and after the total hysterectomy<br>|
| Other: 2<br>105 womens with an intraepithelial lesion | Other: 2 cervical takings and 2 sampling of blood<br>* cervical taking during the cervical conization 2 sampling of blood before and after the intervention<br>|
| Other: 3<br>105 womens with a cancer of the cervix | Other: 2 cervical takings and 2 sampling of blood<br>* cervical taking during the pelvien examination under general anesthetic realized systematically during the balance sheet of pre-therapeutic evaluation 2 sampling of blood before and after the intervention<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Estimate the meaning of a viral load raised in HPV oncogenic |  | 36 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To estimate the correlation enters the vorale load of the HPV oncogenic and the severity of the cervical hurt |  | 36 months | 
| Estimate the correlation between the viral load of the HPV oncogenic and the level of transcription of the genes coding for oncoproteines E6 and E7 |  | 36 months | 
| Estimate the correlation between the viral load in HPV oncogenic and the integration of the viral genome in that of the infected cells |  | 36 months | 
| Estimate the correlation between a viral load raised in HPV oncogenic and a presence of HPV in the circulating blood of the infected patients |  | 36 months |