Protein Requirements for Active Adolescent Males (IAAO-AM)

Study Overview
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Brief Summary
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Protein requirements in active individuals have been suggested to be greater than the current recommended dietary allowance (RDA). Nutritional requirements for dietary amino acids in adolescents have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirements. As a result, there is a need to re-evaluate recommendations in order to characterize how dietary amino acid needs may be modulated by physical activity. Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than the RDA based on nitrogen balance data. The purpose of this study is to measure the protein requirement in adolescent, males using the IAAO technique in the presence of a variable intensity exercise stimulus.

Official Title
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Evaluation of Protein Requirements in Active Adolescent Males

Conditions
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Healthy

Intervention / Treatment
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* Dietary Supplement: Amino Acid Intake


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria (Adolescents): Healthy, active adolescent who regularly participates in moderate to vigorous physical activity 5 times a week for a minimum of 1 hour. Being -0.5 to +1.0 years from peak height velocity, determined by the ratio of standing to sitting height, with the maximal age for adolescents being 16 years. Obtaining a minimum level of 8.3 on the Beep Test Exclusion Criteria (All Subjects): Inability to meet health and physical activity guidelines according to the Physical activity readiness questionnaire (PAR-Q+) and Physical Activity Questionnaire, an inability to adhere to any of the protocol guidelines (i.e. caffeine consumption) a biological age falling outside of the aforementioned ranges

Ages Eligible for Study
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Minimum Age: 10 Years
Maximum Age: 15 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Active, Adolescent males<br>Subjects will receive different levels of amino acid intakes, varying from 0.2-2.67 g/kg/d | Dietary Supplement: Amino Acid Intake<br>* Amino acid intakes will be varied at 0.2-2.67 g/kg/d<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 13CO2 ([13]Carbon Dioxide) Excretion (µmol/kg/h) | Each trial day will assess a different protein intake with 13CO2 excretion defining the level of protein adequacy. Each trial day will be separated by  1wk washout with all trial being completed in approximately 3 months. | 4 hours per study day | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| [13C]Phenylalanine Oxidation (µmol/kg/h) | Each trial day will assess a different protein intake with 13CO2 excretion defining the level phenylalanine oxidation being used as a secondary marker of protein adequacy. Each trial day will be separated by  1wk washout with all trial being completed in approximately 3 months. | 4 hours per study day | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Increased Metabolic Requirement