Development of Cellular Models for Osteoblast Response Study to Adipocytic Secretions in an Osteoporosis Context.

Study Overview
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Brief Summary
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The recent studies suggest that secretions from medullary adipocytes are involved in the mechanisms of bone loss in osteoporosis (OP) through their actions on neighbouring osteoforming cells, the osteoblasts. The objective of the research is the development of new cellular models representing the aging skeleton to confirm this hypothesis. To this end, osteoblasts will be isolated from human bone fragments coming from femoral heads discarded during total hip replacement surgery. The osteoblastic response to secreted factors released from medullary adipocytes of commercial origin will be analysed using conditioned media incubations. This phenotypic response will be quantified for each subject through the analysis of gene expression levels. Inter-subject phenotype variations will be related to bone density and microarchitecture data obtained by X-ray microtomography. This will assess the existence of a correlation between the osteoblast response to adipocyte secretions and the degree of osteoporosis of the subject from whom the cells are derived.

Official Title
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Development of Cellular Models for Osteoblast Response Study to Adipocytic Secretions in an Osteoporosis Context.

Conditions
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Osteoporosis

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Adult subject Surgical indication for hip prosthesis implantation Affiliation to a social insurance regime Exclusion Criteria: Rejection of participation Diagnosed bone diseases other than osteoarthritis or osteoporosis Cancer Pregnant Women Subject under guardianship or trusteeship Subject unable to understand the study.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| the level of gene expression of adipocyte markers such as PPARG and leptin | The effect of adipocyte secretion products on osteoblasts will be evaluated by incubating osteoblasts from human bone biopsies with commercially available adipocyte conditioned cell media. Phenotypic changes in osteoblastic cells will then be evaluated by analyzing their gene expression profile and comparing them with those of osteoblasts incubated in unconditioned media. The selected criteria for judging osteoblast alteration will be the measurement of the level of gene expression of adipocyte markers such as PPARG and leptin. | Baseline | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Inter-subject phenotype variations by X-ray microtomography | Inter-subject phenotype variations will be related to quantitative bone microarchitecture data determined by X-ray microtomography. | Baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Bone, Osteoporosis, Osteoblast, Adipocyte, Cellular models