Research on Precise Immune Prevention and Treatment of Glioma Based on Multi-omics Sequencing Data

Study Overview
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Brief Summary
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This project intends to use multiple types of biological samples from glioma patients and mouse intracranial tumor models as research objects, and comprehensively apply a series of omics sequencing technologies and molecular biology technologies to jointly define the following research objectives :

Detailed Description
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Through high-throughput sequencing technologies such as， genome sequencing, transcriptome sequencing and single-cell sequencing, Proteomics，Metabonomics and Metagenomics， the similarities and differences of the glioma immune microenvironment within and between individuals were revealed from the multi-dimensional and big data level, and the immune molecular typing of glioma was established. Establish and verify that the neoantigen polypeptides predicted by AI algorithm after analyzing multi-omics data (genome, transcriptome, etc.) have good killing effect on individual tumor cells. To establish an intelligent evaluation model of glioma immunotyping by integrating imaging, pathology, high-throughput sequencing data and other omics information, and to provide a visual tool for monitoring changes in the intratumoral microenvironment of glioma patients, with the expectation of helping timely intervention of immunotherapy in patients with recurrence.

Official Title
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Research on Precise Immune Prevention and Treatment of Glioma Based on Multi-omics Sequencing Data

Conditions
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Transcriptomics, Radiomics, Glioma

Intervention / Treatment
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* Procedure: surgery


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The patients with glioma in the Department of Neurosurgery of Huashan Hospital Affiliated to Fudan University who meet the following three conditions can be enrolled They were 18-80 years old, male and female; The pathological diagnosis was glioma; On the basis of not affecting the clinical routine diagnosis, tissue (6 mm * 6 mm) or paraffin section (5 pieces) can be used for research; Sign informed consent or ethics committee approval Exclusion Criteria:

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| glioma patients<br>glioma patients with routine surgery | Procedure: surgery<br>* surgery<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Overall Survival（OS），month | Survival time from surgery to death in 120 patients with glioma. | 24 month | 
| Progress Free Survival （PFS），month | Survival time from surgery to first progression or death from any cause in patients with glioma. | 24 month | 
| Tumor Mutation Burden（TMB），mutations/mb, drived from Genomic sequencing | the level of gene mutation，including TERT、GMEM、IDH.etc. | 24 month | 
| Reads Per Kilobase Million（RPKM），KB^(-1), drived from Transcriptome sequencing | the expression level of RNA,including mRNA、 miRNA、circRNA.etc | 24 month | 
| expression level of protein , drived from Protein sequencing | the expression level of protein, post-translational modification and protein-protein interaction，including PD1、PDL1、CD4、CD8.etc. | 24 month | 
| expression level of low molecular weight protein，drived from Metabonomics | the expression level and interaction of different Metabolites，including D-2-Hydroxyglutarate. etc. | 24 month | 
| expression level of DNA，drived from Metagenomics | Differences about DNA expression of all microorganisms in the environment | 24 month | 
| frequency of TCR/BCR clone，drived from Immunomics | different expression level and interaction of Immune molecules，including TCR、BCR、antibody.etc. | 24month | 
| expression level of protein，drived from paraffin-embedded specimens， retrospectively collected. | different expression level of proteins in Gliomas with different grades and molecular subgroups (1000 cases) | 24month |