Neopterin Dosage in the Eye

Study Overview
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Brief Summary
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The purpose of the study is to determine whether neopterin can be dosed in the fluid of the eye's anterior chamber for patient with non-inflammatory and non-infectious ophthalmic pathology.

Detailed Description
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The study will be explained to subjects who are about to undergo ophthalmologic surgery. They will sign an informed consent and be included in the study. Urine sample will be collected specifically for the study. Eye's anterior chamber fluid will also be collected during the surgery as it is the usual practice (the sample is used for the neopterin dosage instead of being thrown away). These samples will be centrifuged, frozen in a -80° C freezer. At the end of the study, all the samples will be sent to the central Lab for the neopterin dosage analysis. Results will be communicated to the principal investigator afterward.

Official Title
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Appreciation of the Possibility of Dosing Neopterin in Eye Liquids. Pilot Study of Neopterin Dosage in Eye Liquids.

Conditions
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Cataract

Intervention / Treatment
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* Biological: Samples


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age ≥ 18 years-old Non inflammatory or non infectious ophthalmic pathology requiring puncture of eye's anterior chamber fluid. Minimum of 200 microliter sample Immunocompetent subjects Signed and dated informed consent Samples management in accordance with the pre-analytical conditions specified in the protocol Non-inclusion criteria: Age < 18 years-old Subjects treated with intraocular corticoids, antibiotics or anti-inflammatory, locally or in systemic injection, prior to inclusion. Exclusion Criteria: Subjects who exercised his right of withdrawal Study suspended on principal investigator, sponsor or health authorities' demand. Deceased subjects

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Neopterin Dosage<br>Eye's anterior chamber fluid and urines are sampled for posterior neopterin analysis | Biological: Samples<br>* Eye's anterior chamber fluid and urines are sampled for posterior neopterin analysis<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Detection and dosage of neopterin in the eye | HCL will detect and dose the neopterin in the eye's anterior chamber fluid | 3 month | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Neopterin concentration comparison between study values and reference value. | The reference values are standards established by the Neurobiology Lab from Lyon CHU which is the central lab that will performed the neopterin dosages. | 3 month | 
| False positives identification and elimination. | False positives will be determined by calibration of creatinine dosage in eye and urine samples. | 3 month | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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eye's anterior chamber fluid, cerebrospinal fluid, inflammatory pathology