Effect of Degranulation Needle With Different Diameters on the Rate Good Embryo in IVF

Study Overview
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Brief Summary
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Good-quality embryos are critical for the success of in vitro fertilization (IVF). But, to date, there is no report of the effect of different diameters denuding pipette on the embryos quality. To investigate the effect of denuding pipette in IVF outcomes, we plan to recruit women undergoing IVF treatment cycles and classified them into two groups according to the different diameters. Consequently, the rate of fertilization, zygotes cleaved, top quality embryos on D3, and blastocysts obtained were recorded.

Detailed Description
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Good-quality embryos are critical for the success of in vitro fertilization (IVF). But, to date, there is no report of the effect of different diameters denuding pipette on the embryos quality. To investigate the effect of denuding pipette in IVF outcomes, we plan to recruit women undergoing IVF treatment cycles and classified them into two groups according to the different diameters. The participants were divided into two group. Odd-numbered days the zygotes were degranulated with the 140 microns needles. Even-numbered days the zygotes were degranulated with the 150 microns needles. Consequently, the rate of fertilization, zygotes cleaved, top quality embryos on D3, and blastocysts obtained were recorded.

Official Title
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Effect of Degranulation Needle on the Rate Good Embryo in IVF

Conditions
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Embryo

Intervention / Treatment
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* Device: 140 microns denuding pipette
* Device: 150 microns denuding pipette


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: first cyele of IVF treatment Exclusion Criteria: oocyte number less than 4 oocyte number over than 25

Ages Eligible for Study
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Minimum Age: 22 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Odd-numbered days<br>Once odd-numbered days the zytoges of IVF cycle were collected. The 140 microns denuding pipette was used. | Device: 140 microns denuding pipette<br>* the degranulated needle size was 140 microns<br>|
| Experimental: Even-numbered days<br>Once even-numbered days the zytoges of IVF cycle were collected. The 150 microns denuding pipette was used. | Device: 150 microns denuding pipette<br>* the degranulated needle size was 150 microns<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| quality of embryo | embryo quality assessed by good embryo rate | one year | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| clinical outcome - clinical prengancy rate | clinical prengancy rate | three years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Embryos quality, In vitro fertilization, Denuding pipette