Diagnostic Importance of the Circulating Human Leucine-rich α-2-glycoprotein in Acute Appendicitis in Pediatric Patients

Study Overview
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Brief Summary
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Acute appendicitis is a common and potentially serious medical condition which can be difficult to diagnose, especially in pediatric patients. To help recognize patients with acute appendicitis, number of laboratory and radiological test are used. Previous research has shown that Circulating Human Leucine-rich α-2-glycoprotein (LGR1) in the blood can be elevated in pediatric patients with acute appendicitis. The aim of this research is to analyse whether LGR1 levels in saliva can be used as a less invasive diagnostic method in pediatric patients with suspected acute appendicitis.

Official Title
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Diagnostic Importance of the Circulating Human Leucine-rich α-2-glycoprotein Analysed From Blood and Saliva in Children With Acute Appendicitis

Conditions
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Appendicitis Acute

Intervention / Treatment
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* Diagnostic Test: Blood specimen collection
* Diagnostic Test: Saliva specimen collection


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: children with age from 5 do 17 with acute abdominal pain children with clinical signs of appendicitis Exclusion Criteria: previous diagnosis of chronic and/or malignant disease children which had previous abdominal surgery pregnancy

Ages Eligible for Study
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Minimum Age: 5 Years
Maximum Age: 17 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Patients with acute appendicitis<br>Patients with acute appendicitis, after surgery and with pathohistological specimen confirmation. | Diagnostic Test: Blood specimen collection<br>* Blood specimen collection for analysis of CRP (C-reactive protein), WBC (White blood cells) and LRG1 (Circulating Human Leucine-rich α-2-glycoprotein) levels<br>Diagnostic Test: Saliva specimen collection<br>* Saliva specimen collection for analysis of LRG1 (Circulating Human Leucine-rich α-2-glycoprotein) levels<br>|
| Patients without acute appendicitis<br>Patients without acute appendicitis, after laboratory, clinical and/or radiological exclusion of acute admittance. | Diagnostic Test: Blood specimen collection<br>* Blood specimen collection for analysis of CRP (C-reactive protein), WBC (White blood cells) and LRG1 (Circulating Human Leucine-rich α-2-glycoprotein) levels<br>Diagnostic Test: Saliva specimen collection<br>* Saliva specimen collection for analysis of LRG1 (Circulating Human Leucine-rich α-2-glycoprotein) levels<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Level of LGR1 protein in blood | Levels of LGR1 protein in blood and saliva as measured by ELISA test | 72 hours | 
| Level of LGR1 protein saliva | Levels of LGR1 protein in saliva as measured by ELISA test | 72 hours |