Fat-induced Release of GLP-1 in Humans

Study Overview
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Brief Summary
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This study compared the incretin-releasing effect of C8-diet oil and tributyrin with that of 20 ml olive oil on a molar basis in human volunteers.

Detailed Description
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A structured triglyceride (1,3-di-octanoyl-2-oleoyl-glycerol, called C8-diet oil), which upon digestion results in generation of two medium-chain fatty acids and 2-oleoyl-glycerol (2-OG), may lead to activation of GPR119 only, since medium fatty acids are poor agonists for fatty acid receptors.

Official Title
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Short Fatty Acids-induced Release of GLP-1 in Humans

Conditions
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Type 2 Diabetes

Intervention / Treatment
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* Dietary Supplement: Tributyrin
* Dietary Supplement: C8-diet oil
* Dietary Supplement: Olive oil
* Dietary Supplement: Carrot


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Caucasian healthy male subjects Body mass index (BMI) 19 to 25 kg/m2 Informed consent Exclusion Criteria: Anaemia Diabetes mellitus Gastrointestinal diseases or operations interacting with intestinal absorption

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: A meal containing carrot and tributyrin<br>A meal containing 200 g grated carrot and 0.0216 mol tributyrin; blood test taken from 0 - 2 hours postprandial | Dietary Supplement: Tributyrin<br>* a triglyceride containing three C4-acids<br>Dietary Supplement: Carrot<br>* mainly water and glucose<br>|
| Active Comparator: A meal containing carrot and C8-diet oil<br>A meal containing 200 g grated carrot and 0.0216 mol of C8-diet oil; blood test taken from 0 - 2 hours postprandial | Dietary Supplement: C8-diet oil<br>* a triglyceride containing 2 C8-fatty acids and one oleic acid at sn-2 position<br>Dietary Supplement: Carrot<br>* mainly water and glucose<br>|
| Active Comparator: A meal containing carrot and olive oil<br>A meal containing 200 g grated carrot and 0.0216 mol of olive oil; blood test taken from 0 - 2 hours postprandial | Dietary Supplement: Olive oil<br>* a triglyceride containing mostly three oleic acids<br>Dietary Supplement: Carrot<br>* mainly water and glucose<br>|
| Placebo Comparator: A meal containing carrot<br>A meal containing 200 g grated carrot; blood test taken from 0 - 2 hours postprandial | Dietary Supplement: Carrot<br>* mainly water and glucose<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| plasma GLP-1 |  | 2 hours postprandial | 
| plasma GIP |  | 2 hours postprandial | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| plasma insulin |  | 2 hours postprandial | 
| plasma PYY |  | 2 hours postprandial | 
| plasma glucose |  | 2 hours postprandial | 
| plasma neurotensin |  | 2 hour postprandial | 
| plasma CCK |  | 2 hour postprandial | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Short fatty acids, 2-oleoyl glycerol, GLP-1 secretion, C8-diet oil, GIP secretion