Leukemia Inhibitory Factor Level in Intrauterine Growth Restriction Neonates

Study Overview
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Brief Summary
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To see if there is a relationship between the level of LIF in IUGR fetuses and compared to the level of LIF in AGA fetuses

Detailed Description
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During embryonic development, there are several cytokines such as: LIF (Leukemia inhibitory factor), ciliary neurotrophic factor (CNTF), epidermal growth factor family (EGF), neuregulin 1 (NRG1) and transforming growth factor β (TGFβ) that were found are associated with neurogenesis and differentiation of brain cells . LIF is a cytokine that is essential for the development of the central nervous system, and has recently been shown in rats that maternal LIF stimulates placental ACTH that in turn promotes secretion of fetal LIF from nRBC , which in turn promotes brain development of the fetus Other studies on IUGR (Intra Uterine Growth Restriction) have shown that IUGR fetuses have more CP (Cerebral palsy) than those who were AGA (Average for Gestational Age) The hypothesis is that LIF is related to the proper development of the nervous system in the fetus and by testing cord blood of embryos IUGR and the placenta we will find changes in LIF and ACTH compared with AGA fetuses level. Studies have also shown a relationship between CP and IUGR, but the mechanism for this relationship is unclear. The purpose of our research is to see if there is a relationship between the level of LIF in IUGR fetuses and compared to the level of LIF in AGA fetuses, in one of two ways: Changes in the secretion of ACTH in placenta Changes in levels of LIF in the umbilical cord blood

Official Title
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Comparison of LIF (Leukemia Inhibitory Factor) Level Between Neonates Who Are IUGR (Intra Uterine Growth Restriction) and Those Who Are AGA (Average for Gestational Age)

Conditions
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to Compare LIF Level in Cord Blood of Embryo's That Are IUGR to Those That Are AGA

Intervention / Treatment
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* Other: blood sample and tissue sample


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Women that the Fetal weight estimate puts the fetus bellow the 10th percentile and those that the fetal weight estimate are between 20th and 80th percentiles Exclusion Criteria: Women who do not agree known Genetic diseases of abnormalities in the fetus

Ages Eligible for Study
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Minimum Age: 16 Years
Maximum Age: 45 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| control<br>Women with IUGR embryos so that their fetal weight estimate puts them in a percentile bellow 10th percentile | Other: blood sample and tissue sample<br>* After birth and fetal umbilical cord disconnection, a blood sample will be taken from the umbilical cord (cord blood) of IUGR and of AGA fetuses (5cc) for cytokine ELISA testing. Also a small sample of the placenta will be examined in order to assess the level of ACTH protein in IUGR and AGA embryos<br>|
| IUGR<br>Women with AGA embryos so that their fetal weight estimate puts them in a percentile between 20th and 80th percentiles | Other: blood sample and tissue sample<br>* After birth and fetal umbilical cord disconnection, a blood sample will be taken from the umbilical cord (cord blood) of IUGR and of AGA fetuses (5cc) for cytokine ELISA testing. Also a small sample of the placenta will be examined in order to assess the level of ACTH protein in IUGR and AGA embryos<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| LIF Protein Level in IUGR Neonates vs. AGA neonates |  | 2 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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LIF IUGR