A Phase Ib Safety lead-in, Followed by Phase II Trial of ADG106 in Combination With Neoadjuvant Chemotherapy in HER2 Negative Breast Cancer

Study Overview
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Brief Summary
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This is an open label, lead in phase Ib dose confirmation study in patients with advanced solid tumors, followed by a phase II single arm study as neoadjuvant therapy in stage I-III HER2 negative breast cancer. Primary Objectives To determine the safety profile of combination of ADG106 with dose dense doxorubicin/cyclophosphamide, and with weekly paclitaxel. To determine the Recommended Phase 2 Dose (RP2D) of ADG106 in combination with dose dense doxorubicin/cyclophosphamide, and with weekly paclitaxel. To evaluate biological changes on immunohistochemistry in HER2 negative breast cancer after treatment with ADG106 alone and in combination with chemotherapy. Secondary Objectives To determine the efficacy of combination of ADG106 with standard neoadjuvant combination chemotherapy in HER2 negative breast cancer: objective response rates. To correlate tumor and plasma biomarkers with efficacy outcomes.

Detailed Description
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The phase Ib segment will be carried out with a standard 3+3 dose de-escalation design. Patients with advanced/ metastatic solid organ cancers will be enrolled in 2 parallel cohorts. Cohort 1 will receive ADG106 in combination with dose dense doxorubicin/ cyclophosphamide (AC). Cohort 2 will receive ADG106 in combination with weekly paclitaxel. In the phase II portion, patients with stage I-III HER2 negative breast cancer planned for neoadjuvant chemotherapy will be enrolled. Patients will be treated with neoadjuvant chemotherapy (ddAC followed by paclitaxel for 12 weeks) combined with ADG106, before definitive breast cancer surgery

Official Title
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A Phase Ib Safety lead-in, Followed by Phase II Trial of ADG106 in Combination With Neoadjuvant Doxorubicin and Cyclophosphamide Followed by Paclitaxel in Stage I-III HER2 Negative Breast Cancer

Conditions
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HER2-negative Breast Cancer, Advanced Solid Tumor

Intervention / Treatment
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* Drug: ADG106
* Drug: Doxorubicin
* Drug: Cyclophosphamide
* Drug: Paclitaxel


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients may be included in the study only if they meet all of the following criteria: All patients must sign an informed consent in accordance with local institutional guidelines. 18 years and above of age. Estimated life expectancy of at least 12 weeks. Has recovered from acute toxicities from prior anti-cancer therapies (phase Ib). a) Phase Ib: Patients with histologically or cytologically confirmed advanced or metastatic solid tumors who have radiological evidence of progressive disease on study entry that are deemed likely to benefit from either dose dense doxorubicin/ cyclophosphamide or weekly paclitaxel. There is no upper limit on the number of prior treatments provided all inclusion/ exclusion criteria are met. Hormone ablation therapy is considered an anti-cancer regimen. Radiation therapy and surgery are not considered anti-cancer regimens. Prior receipt of immunotherapy is allowed. b) Phase II: Untreated stage I-III HER2 negative breast cancer patients who are planned for neoadjuvant chemotherapy followed by definitive breast cancer surgery. Measurable disease by RECIST 1.1 criteria. Eastern Cooperative Oncology Group (ECOG) Performance Status of 0-1. Left ventricular ejection fraction of ≥ 50% for Cohort 1 in phase Ib and all patients in phase II. Adequate bone marrow function and organ function within 2 weeks of study treatment. Adequate hematologic function defined as: Absolute neutrophil count (ANC) ≥ 1.5 x 109/L Platelets ≥ 100 x 109/L Hemoglobin ≥ 9 x 109/L Adequate hepatic function defined as: Bilirubin < 1.5 times the upper limit of normal (ULN) ALT or AST < 2.5 times ULN (or < 5 times ULN with presence of liver metastases) Adequate renal function defined as: - Calculated creatinine clearance of ≥ 60 mL/min, calculated using the formula of Cockroft and Gault: (140-Age) x Mass (kg)/(72 x creatinine mg/dL); multiply by 0.85 if female. Adequate coagulation function defined as: Activated partial thromboplastin time (aPTT) ≤1.5 x ULN International normalized ratio (INR) ≤1.5 x ULN (Exception: INR 2 to ≤3 x ULN is acceptable for patients on warfarin anticoagulation Patients with reproductive potential must use an approved contraceptive method if appropriate (e.g., intrauterine device, birth control pills, or barrier device) during and for three months after the study. Females with childbearing potential must have a negative serum pregnancy test within 7 days prior to study enrolment. Able to comply with study related procedures. Exclusion Criteria: Patients will be excluded from the study for any of the following reasons: Treatment within the last 30 days with any investigational drug. Concurrent administration of any other tumor therapy, including cytotoxic chemotherapy, hormonal therapy, and immunotherapy. Major surgery within 28 days of study drug administration. Active infection that in the opinion of the investigator would compromise the patient's ability to tolerate therapy. Serious concomitant disorders that would compromise the safety of the patient or compromise the patient's ability to complete the study, at the discretion of the investigator. Active, known or suspected autoimmune disease. Subjects with type I diabetes mellitus, hypothyroidism only requiring hormone replacement, skin disorders (such as vitiligo, psoriasis, or alopecia) not requiring systemic treatment, or conditions not expected to recur in the absence of an external trigger are permitted to enrol. Subjects with a condition requiring systemic treatment with either corticosteroids (>10mg daily prednisone equivalent) or other immunosuppressive medications within 14 days of randomization. Inhaled or topical steroids, and adrenal replacement steroid >10mg daily prednisone equivalent, are permitted in the absence of active autoimmune disease. Subjects with interstitial lung disease that is symptomatic or may interfere with the detection or management of suspected drug-related pulmonary toxicity. Known history of testing positive for human immunodeficiency virus (HIV) or known acquired immunodeficiency syndrome (AIDS). Active hepatitis B (positive hepatitis B surface antigen [HBsAg]) or HCV (hepatitis C virus) [positive HCV RNA]) Patients with past HBV infection or resolved HBV infection (defined as the presence of hepatitis B core antibody [HBcAb] and absence of HBsAg) are eligible. HBV DNA must be obtained in these patients prior to randomization. HBV carriers or those patients requiring antiviral therapy are not eligible to participate. Patients positive for HCV antibody are eligible only if PCR is negative for HCV RNA. HBV carriers or those subjects receiving antiviral treatment of hepatitis B virus or Hepatitis C are not eligible. Pregnancy. Breast feeding. Second primary malignancy that is clinically detectable at the time of consideration for study enrolment. The exception is patients in phase II with two or more primary invasive breast cancers that are both HER2 negative and where both cancers are amenable to repeated biopsy. In this case, each tumor will be biopsied and assessed separately for treatment response. Symptomatic brain metastases. History of significant neurological or mental disorder, including seizures or dementia. Unable to comply with study procedures. Phase II cohort: Prior treatment for locally advanced or metastatic breast cancer. Patients with known underlying hemoglobinopathies (e.g., thalassemia). Note: patients without known hemoglobinopathies do not specifically need to be screened for hemoglobinopathies in the absence of clinical suspicion). History of life-threatening hypersensitivity or known to be allergic to protein drugs or recombinant proteins or any ingredients contained in the ADG106 drug formulation (succinic acid, arginine, polysorbate 80 and hydrochloric acid). Peripheral neuropathy grade ≥2. Live viral vaccine therapies within 4 weeks prior to the first dose of study drug.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 99 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Intervention Model: Sequential Assignment
Interventional Model Description: Phase Ib: A 3+3 dose escalation design will be used to determine the RP2D dose of ADG106 in combination with ddAC and paclitaxel respectively. Phase II: ADG106 will be combined with ddAC followed by paclitaxel as neoadjuvant chemotherapy in patients with stage I-III HER2 negative breast cancer.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: ADG106 combined with dose dense Doxorubicin and Cyclophosphamide (Phase Ib)<br>Intravenous ADG106 + 2 weekly doxorubicin and cyclophosphamide | Drug: ADG106<br>* Administered as an intravenous infusion over 60-90 minutes in the initial cycle and over 30 minutes in subsequent cycle if well tolerated.<br>Drug: Doxorubicin<br>* Administered as an intravenous infusion.<br>* Other names: Adriamycin;Drug: Cyclophosphamide<br>* Administered as an intravenous infusion.<br>* Other names: Cytophosphane;|
| Experimental: ADG106 combined with Paclitaxel (Phase Ib)<br>Intravenous ADG106 + weekly paclitaxel | Drug: ADG106<br>* Administered as an intravenous infusion over 60-90 minutes in the initial cycle and over 30 minutes in subsequent cycle if well tolerated.<br>Drug: Paclitaxel<br>* Administered as an intravenous infusion.<br>* Other names: Taxol;|
| Experimental: ADG106 combined with dose dense Doxorubicin and Cyclophosphamide follow by Paclitaxel (Phase II)<br>Intravenous ADG106 combined with two weekly doxorubicin and cyclophosphamide followed intravenous ADG106 combined with weekly paclitaxel | Drug: ADG106<br>* Administered as an intravenous infusion over 60-90 minutes in the initial cycle and over 30 minutes in subsequent cycle if well tolerated.<br>Drug: Doxorubicin<br>* Administered as an intravenous infusion.<br>* Other names: Adriamycin;Drug: Cyclophosphamide<br>* Administered as an intravenous infusion.<br>* Other names: Cytophosphane;Drug: Paclitaxel<br>* Administered as an intravenous infusion.<br>* Other names: Taxol;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of participant with treatment related toxicities | Toxicities will be graded using the Common Terminology Criteria for Adverse Events (CTCAE) toxicity grading version 5.0. | From enrolment till 30 days after last dose of study treatment | 
| Histological response after neoadjuvant ADG106 + chemotherapy | Biological changes on immunohistochemistry will be evaluated using paraffin-embedded tumor specimens. | After 20 weeks of neoadjuvant chemotherapy | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Objective response rate in Phase Ib | Complete and partial clinical response will be measured by RECIST 1.1. | At the end of every 3 cycles up to 24 weeks, at the end of every 6 cycles after 24 weeks up to 60 weeks (each cycle is 2 weeks) | 
| Overall survival in Phase Ib | Defined as the time from the date of study enrolment to the date of death from any cause, regardless of whether the death occurred during the study or following treatment discontinuation. | From enrolment till date of death or final follow up visit (maximum 1 year after last treatment dose) | 
| Correlation of plasma biomarkers with efficacy outcome in Phase Ib | Correlation of biomarkers with Objective Response Rate, Progression Free Survival, Overall Survival | baseline, at the end of week 1, 2, 4, 6, 8, 10, 12, 14, 18, 30, 42, 54, 66 | 
| Progression free survival in Phase Ib | Defined as the time from the date of study enrolment to the first date of documented disease progression. | From enrolment till disease progression or date of death or final follow-up visit (maximum 1 year after last treatment dose). | 
| Clinical response rate in Phase II | Clinical response rate will be measured by calipers | baseline, at the end of 2 weeks, 4 weeks, 8 weeks, 10 weeks, 12 weeks of treatment. | 
| Pathological complete response rate in Phase II | Defined as any patient who demonstrates no histological evidence of invasive tumor in the primary breast site as well as in resected axillary lymph nodes. | after 20 weeks neoadjuvant chemotherapy | 
| Relapse free survival in Phase II | Defined as the time from the date of study enrolment to the first date of documented disease relapse. | From enrolment to final follow up visit (maximum 6 years from last treatment dose) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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HER2-negative Breast Cancer, ADG106, neoadjuvant doxorubicin and cyclophosphamide, paclitaxel