Serological Evaluation of 77A/G and 11A/12A Gene of MMP-13 in Periodontitis Affected and Healthy Individuals

Study Overview
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Brief Summary
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50 periodontitis 50 healthy individuals serological evaluation will done to see association of 77A/G AND 11A/12A gene polymorphisms of MMP-13

Detailed Description
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The study will be conducted on 100 individuals 50 chronic periodontitis and 50 healthy individuals will be recruited from the Out Patient Department of Periodontics in the study. An informed consent will be obtained from patients who wish to participate in the study. Patients will be categorized by Periodontal Disease Index (PDI) by P. Ramfjord(1959) After clinical examination, blood samples will be collected and assessed for MMP 13 Gene Polymorphisms. Subjects will be selected according to the study criteria and divided into two groups (50 each). Test group Control group

Official Title
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A Serological Evaluation of 77A/G and 11A/12A Associated Gene Polymorphisms of MMP-13 in Periodontitis Affected and Healthy Individuals

Conditions
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Generalized Adult Periodontitis

Intervention / Treatment
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* Genetic: blood samples collected from all patients


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria:• Test group Patients with chronic periodontitis classified according to American Academy of Periodontology ( AAP) 1999 Subjects aged between 30-55 years Control group Systemically and periodontally healthy individuals of same age group Exclusion Criteria: Present and past Smokers Presence of any systemic diseases. Oral diseases other than Periodontitis With a previous history of periodontal treatment in the past 6 months and subjects under any medications. Family history of any diagnosed genetic disorders.

Ages Eligible for Study
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Minimum Age: 30 Years
Maximum Age: 55 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| test<br>50 periodontitis patients • Patients will be categorized by Periodontal Disease Index (PDI) by P. Ramfjord(1959) blood samples collected from all patients serum separated and evaluated for77A/G AND 11A/12A associated gene polymorphism of Matrix Metallo Proteinase( MMP)-13 in all the patients. studies showing that there is co relation of these genes with increase inflammation. | Genetic: blood samples collected from all patients<br>* blood samples collected from brachial pluxes<br>|
| control<br>50 periodontally healthy individuals blood samples collected from all patients serum separated and evaluated for77A/G AND 11A/12A associated gene polymorphism of Matrix Metallo Proteinase (MMP)-13 in all the patients | Genetic: blood samples collected from all patients<br>* blood samples collected from brachial pluxes<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| to evaluate the association of 77A/G AND 11A/12A gene polymorphism of mmp-13 in periodontitis effected and healthy individuals | collect DNA samples from serum and evaluate for gene polymorphism | 6 months |