Biomarkers in Tumor Samples From Younger Patients With Neuroblastoma

Study Overview
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Brief Summary
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RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies biomarkers in tumor samples from younger patients with neuroblastoma.

Detailed Description
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OBJECTIVES: To determine the expression of UBE4B, hrs, and members of the ubiquitination protein complex in previously banked primary neuroblastoma tumor samples from the Children's Oncology Group. To determine the correlation of expression levels and cellular localization of the UBE4B and hrs proteins with growth factor-receptor tumor cell-surface expression. To determine the 1p36 deletion status by fluorescence in situ hybridization (FISH) analysis and their association with expression levels of UBE4B and hrs in previously banked primary neuroblastoma tumor samples from the Children's Oncology Group. OUTLINE: Banked tumor tissue samples are analyzed for expression of UBE4B, hrs, members of the ubiquitination protein complex, growth factor receptors, and 1p36 deletion status by immunohistochemistry (IHC), fluorescent microscopy, and fluorescence in situ hybridization (FISH).

Official Title
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Proposed Studies on the Role of Growth Factor Receptor Trafficking in Neuroblastoma Pathogenesis

Conditions
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Neuroblastoma

Intervention / Treatment
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* Genetic: fluorescence in situ hybridization
* Genetic: gene expression analysis
* Genetic: microarray analysis
* Genetic: mutation analysis
* Genetic: protein analysis
* Other: immunohistochemistry staining method
* Other: laboratory biomarker analysis
* Other: microscopy


Participation Criteria
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Eligibility Criteria
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DISEASE CHARACTERISTICS: Tumor tissue microarrays from the Children's Oncology Group neuroblastoma tumor bank PATIENT CHARACTERISTICS: Not specified PRIOR CONCURRENT THERAPY: Not specified

Ages Eligible for Study
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Maximum Age: 120 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Genetic: fluorescence in situ hybridization|nan|
|Genetic: gene expression analysis|nan|
|Genetic: microarray analysis|nan|
|Genetic: mutation analysis|nan|
|Genetic: protein analysis|nan|
|Other: immunohistochemistry staining method|nan|
|Other: laboratory biomarker analysis|nan|
|Other: microscopy|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Expression of UBE4B, hrs, and members of the ubiquitination protein complex |  |  | 
| Expression levels and cellular localization of the UBE4B, hrs, and growth factor receptors |  |  | 
| 1p36 deletion status and its association with UBE4B |  |  | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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neuroblastoma