Dietary Nucleotides and Ribose on Muscle Fuel Utilisation

Study Overview
=================
Brief Summary
-----------------
Nucleotides are the building blocks for a variety of molecules, including ATP, which is broken down in the skeletal muscle cells to provide fuel for exercise. Nucleotides can be obtained from the diet, and produced or salvaged by the body. Though nucleotides provide the building blocks for ATP, the sugar ribose has been suggested to be rate limiting for synthesising ATP. Previous studies have shown a decrease in ATP levels in the muscle after intense exercise, and this is linked to fatigue. Other studies have also reported benefits of nucleotide and/or ribose supplementation on exercise performance. However, any potential link between ATP metabolism and exercise performance has not been explored. This study will look at the effects of nucleotide and nucleotide-ribose supplementation in fuel utilisation and performance in skeletal muscle during endurance exercise.

Official Title
-----------------
The Effect of Dietary Nucleotides and Ribose on Muscle Fuel Utilisation and Exercise Performance

Conditions
-----------------
Healthy

Intervention / Treatment
-----------------
* Dietary Supplement: Low nucleotide
* Dietary Supplement: High nucleotide
* Dietary Supplement: High nucletide + Ribose


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: BMI between 18 and 30 Exclusion Criteria: On medication (except contraception) Smokers Diagnosed with any metabolic or cardiovascular conditions Muscle or bone injuries BMI < 18 or > 30

Ages Eligible for Study
-----------------
Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Low nucleotide<br>2 week supplementation with a low nucleotide mycoprotein drink, with added dextrose | Dietary Supplement: Low nucleotide<br>* 2 week, twice daily, supplementation with a nucleotide-depleted mycoprotein drink, with added dextrose<br>|
| Experimental: High nucleotide<br>2 week supplementation with a high nucleotide mycoprotein drink, with added dextrose | Dietary Supplement: High nucleotide<br>* 2 week, twice daily, supplementation with a nucleotide-rich mycoprotein drink, with added dextrose<br>|
| Experimental: High nucletide + Ribose<br>2 week supplementation with a high nucleotide mycoprotein drink, with added ribose | Dietary Supplement: High nucletide + Ribose<br>* 2 week, twice daily, supplementation with a nucleotide-rich mycoprotein drink, with added ribose<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in muscle ATP concentrations |  | After each supplementation cycle (day 15 of each cycle): baseline (fasted and before exercise), immediately after glycogen depleting exercise (after exercising for 1:30 - 2 hours), immediately after 15 minute time trial and after 3-hour recovery | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes in muscle glycogen concentrations |  | After each supplementation cycle (day 15 of each cycle): baseline (fasted and before exercise), immediately after glycogen depleting exercise (after exercising for 1:30 - 2 hours), immediately after 15 minute time trial and after 3-hour recovery | 
| Blood lactate concentrations |  | After supplementation cycle (day 15): baseline and at 15 minute intervals up 1 hour during glycogen depleting exercise | 
| Blood glucose concentrations |  | After supplementation cycle (day 15): baseline and at 15 minute intervals up 1 hour during glycogen depleting exercise | 
| Serum uric acid concentrations |  | Measured at baseline and of days 6, 8 and 15 of each supplementation cycle |