The Expression of the Ionotropic Glutamate Receptors in Colon of IBS

Study Overview
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Brief Summary
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The study aims to determine the expression of the ionotropic glutamate receptors in colon of irritable bowel syndrome (IBS) patients and evaluate the relationship between the ionotropic glutamate receptors and IBS symptoms.

Detailed Description
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Ionotropic glutamate receptors participate in pain in nervous system. In this study,participants will examine the expression of the ionotropic glutamate receptors in colon of irritable bowel syndrome (IBS) patients and evaluate the relationship between the ionotropic glutamate receptors and IBS symptoms. Participants may futher study the mechanism using cell experiment.

Official Title
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The Expression of the Ionotropic Glutamate Receptors in Colon of IBS

Conditions
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Irritable Bowel Syndrome

Intervention / Treatment
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* Procedure: colonoscopy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients meeting IBS Rome III criteria. Asymptomatic individuals for health surveillance or patients for follow up after polypectomy. Exclusion Criteria: Patients with known cancers or abdominal surgery. Alarm symptoms such as anaemia, gastrointestinal bleeding or obstruction, marked weight loss, abdominal mass. Patients who are unwilling to sign or give the informed consent form. Patients with impaired cardiac, liver or renal function. Patients with coagulopathy. Patients with pregnancy or breastfeeding.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Irritable bowel syndrome<br>Patients meeting Rome III criteria of irritable bowel syndrome. Intervention: colonoscopy. | Procedure: colonoscopy<br>* Patients will undergo colonoscopy and participants will get biopsies from the colon.<br>|
| Normal controls<br>Asymptomatic individuals for health surveillance or patients for follow up after polypectomy. Intervention: colonoscopy. | Procedure: colonoscopy<br>* Patients will undergo colonoscopy and participants will get biopsies from the colon.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| the expression of the ionotropic glutamate receptors | Participants will examine the expression level of ionotropic glutamate receptors using quantitative real-time RT-PCR and western blot ,examine the distribution using immunohistochemy. | 4 months | 
| the relationship between the ionotropic glutamate receptors and IBS symptom | Symptoms will be assessed though the severity of abdominal pain or discomfort and seizure frequency.Participants will evaluate the relationship between the expression level or the distribution of ionotropic glutamate receptors and IBS symptom. If there is a link between them, participants will study the underlying molecular mechanisms. | 6 months | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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IBS, Ionotropic glutamate receptors