Cellular Adoptive Immunotherapy in Treating Patients With Relapsed or Refractory Follicular Non-Hodgkin's Lymphoma

Study Overview
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Brief Summary
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RATIONALE: Cellular adoptive immunotherapy uses a person's white blood cells that are treated in the laboratory to stimulate the immune system in different ways and stop cancer cells from growing. Rituximab and fludarabine may also prevent the body from making an immune response against the laboratory-treated white blood cells that are put back into the body. Interleukin-2 may help the laboratory-treated white blood cells stay in the body longer. Giving cellular adoptive immunotherapy together with rituximab, fludarabine, and interleukin-2 may kill more cancer cells. PURPOSE: This phase I trial is studying the side effects of cellular adoptive immunotherapy in treating patients with relapsed or refractory follicular non-Hodgkin's lymphoma.

Detailed Description
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OBJECTIVES: Primary Determine the safety and feasibility of cellular adoptive immunotherapy using autologous cytotoxic T lymphocytes genetically modified to express a CD19-specific chimeric immunoreceptor gene and HyTK selection/suicide gene in patients with relapsed or refractory follicular non-Hodgkin's lymphoma. Secondary Determine the in vivo persistence of adoptively transferred cytolytic T cells in patients treated with lymphodepleting therapy comprising rituximab and fludarabine. Assess the development of host immune responses against the CD19-specific chimeric immunoreceptor gene and/or HyTK selection/suicide gene. Determine the safety of low-dose interleukin-2 in supporting in vivo persistence of adoptively transferred cytotoxic T cells. Determine the anti-tumor activity of this regimen in these patients. OUTLINE: This is a nonrandomized, open-label, pilot study. Leukapheresis: Patients undergo leukapheresis for collection of peripheral blood mononuclear cells (PBMCs). CD3-positive cytotoxic T lymphocytes (CTLs) are isolated and genetically modified to express a CD19-specific chimeric immunoreceptor and the HyTK fusion protein, and are then expanded in vitro. Lymphodepleting therapy: Patients receive rituximab and fludarabine prior to T-cell infusions. Cellular adoptive immunotherapy and interleukin-2 (IL-2): Patients receive a total of 5 infusions of genetically modified autologous T cells. Patients may receive low-dose IL-2 subcutaneously after infusions 3, 4, and 5. Additional IL-2 therapy: After the completion of the last T-cell infusion, patients with evidence of adoptively transferred T cells may receive additional IL-2. After completion of study treatment, patients are followed periodically for approximately 65 days and then annually for at least 15 years. PROJECTED ACCRUAL: At least 5 patients will be accrued for this study within 3 years.

Official Title
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Pilot/Feasibility Study To Evaluate The Safety Of Cellular Immunotherapy For CD19+ Follicular Lymphoma Using Autologous Cytolytic T Cells Genetically-Modified To Be CD19-Specific And Co-Express HyTK

Conditions
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Lymphoma

Intervention / Treatment
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* Biological: aldesleukin
* Biological: rituximab
* Biological: therapeutic autologous lymphocytes
* Drug: fludarabine phosphate


Participation Criteria
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Eligibility Criteria
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DISEASE CHARACTERISTICS: Histologically confirmed follicular non-Hodgkin's lymphoma (NHL) High-risk disease, as defined by any of the following: Relapsed within 6 months after the last treatment Failed to achieve a complete response during the last treatment Relapsed after prior autologous hematopoietic stem cell transplantation (HSCT) No current transformation of lymphoma (e.g., elements of intermediate- or high-grade lymphoma by biopsy) No active CNS disease by lumbar puncture or radiology scan NOTE: A new classification scheme for adult non-Hodgkin's lymphoma has been adopted by PDQ. The terminology of indolent or aggressive lymphoma will replace the former terminology of low, intermediate, or high grade lymphoma. However, this protocol uses the former terminology. PATIENT CHARACTERISTICS: Age 16 to 70 Performance status Karnofsky 50-100% Life expectancy More than 16 weeks Hematopoietic Absolute neutrophil count > 500/mm^3 Hepatic Bilirubin ≤ 1.5 times upper limit of normal (ULN)* (unless due to Gilbert's disease) ALT ≤ 2.5 times ULN* NOTE: *Unless due to NHL Renal Creatinine ≤ 1.5 times ULN* OR Creatinine clearance ≥ 80 mL/min* NOTE: *Unless due to NHL Immunologic HIV negative Epstein-Barr virus positive No history of allergy or intolerance to ganciclovir Other Negative pregnancy test No history of another malignancy except basal cell skin cancer or carcinoma in situ No other uncontrolled or severe illness that would preclude study participation PRIOR CONCURRENT THERAPY: Biologic therapy No prior allogeneic HSCT No other immunotherapy during and for approximately 65 days after the last T-cell infusion, unless approved by the Principal Investigator (PI) Chemotherapy No other chemotherapy during and for approximately 65 days after the last T-cell infusion, unless approved by the PI Patients may receive chemotherapy after leukapheresis while waiting for CD19-specific T cells to be manufactured Endocrine therapy No systemic corticosteroids during and for approximately 65 days after the last T-cell infusion, unless approved by the PI Radiotherapy Not specified Surgery Not specified Other No concurrent participation in another investigational study No immunosuppression agents or other investigational agents during and for approximately 65 days after the last T-cell infusion, unless approved by the PI

Ages Eligible for Study
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Minimum Age: 16 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Non-Randomized
Masking: None (Open Label)


Arms and Interventions

| Intervention/Treatment |
| --- |
|Biological: aldesleukin|nan|
|Biological: rituximab|nan|
|Biological: therapeutic autologous lymphocytes|nan|
|Drug: fludarabine phosphate|nan|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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recurrent grade 1 follicular lymphoma, recurrent grade 2 follicular lymphoma, recurrent grade 3 follicular lymphoma