Comparison of the Healing Properties on Corneal Cells of Groth Factor-enriched Plasma and Autologous Serum From Aniridia Patients

Study Overview
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Brief Summary
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Keratopathy of patients with aniridia leads to epithelial scarring disorders and a progressive clouding of the cornea linked to this abnormal healing (fibrosis). Treatment with autologous serum is usually undertaken to promote epithelial healing. However, autologous serum does not prevent the formation of fibrosis, whereas growth factor-rich plasma appears to be associated with a reduction in the in vitro expression of fibrosis markers. This study seeks to compare the in vitro healing and anti-fibrotic properties of autologous serum and growth factor rich plasma from aniridia patients and healthy controls.

Detailed Description
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Blood collection from aniridia patients and healthy controls, half of which will be used to make autologous serum and the other half to make growth factor rich plasma, used for in vitro studies. A conjunctival impression will also be taken at the inclusion of the patients.

Official Title
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Comparison of the Healing Properties on Corneal Cells of Groth Factor-enriched Plasma and Autologous Serum From Aniridia Patients

Conditions
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Aniridia

Intervention / Treatment
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* Procedure: Peripheral venous blood sample
* Procedure: Conjunctival impression


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: For patients with aniridia: With aniridia Patient weighing more than 40 kg If the patient is being treated with autologous serum, a delay of 8 weeks after the last blood collection If a blood donation has been made, a delay of 8 weeks must be respected after the last donation For controls: Non-aniridia (no clinical signs) Weighing more than 40 kg Matched to a case for sex and age +/-5 years No known diabetes If a blood donation has been made, a delay of 8 weeks must be respected after the latter Exclusion Criteria: Person benefiting from a legal protection measure Pregnant or breastfeeding woman

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Case patients with aniridia<br> | Procedure: Peripheral venous blood sample<br>* 2.5% of body weight<br>Procedure: Conjunctival impression<br>* Collection of the most superficial conjunctival epithelial cells (desquamating) in a non- or minimally invasive, quick and almost painless way, for a biological analysis of ocular surface diseases. Application of a filter paper on the bulbar conjunctiva, under local anesthesia.<br>|
| Control patients without aniridia<br> | Procedure: Peripheral venous blood sample<br>* 2.5% of body weight<br>Procedure: Conjunctival impression<br>* Collection of the most superficial conjunctival epithelial cells (desquamating) in a non- or minimally invasive, quick and almost painless way, for a biological analysis of ocular surface diseases. Application of a filter paper on the bulbar conjunctiva, under local anesthesia.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| In vitro comparison of the corneal cell healing properties of autologous serum and plasma enriched with growth factors from blood of aniridia patients | The corneal cell healing properties are evaluated by the time to reach confluence (number of hours) of epithelial cells, using a standardized wound healing assay (Incucyte, Essenbioscience). The corneal cell healing properties will be comparated between autologous serum, and plasma enriched with growth factors from blood of aniridia patients | Sampling done at baseline | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Growth factor rich plasma, Conjunctival impression