Vegetable Signature From Human Metabolomics Responses

Study Overview
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Brief Summary
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The purpose of this study is to perform dietary intervention studies on a variety of vegetables.

Detailed Description
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Plant-based foods have always been an important component of a healthy diet. High intake of plant foods may not only maintain normal bodily functions through the provision of dietary fiber, vitamins, and minerals but also relieve symptoms and prevent diseases. Many studies have pointed out that high intake of plant foods may reduce the incidence of non-communicable disease. Past research investigating the effect of phytochemicals has usually been limited to single substances or single foods. However, a number of researchers have pointed out that phytonutrients and dietary components are likely to act synergistically.Health effects and mechanisms of phytonutrients in combination have not been carefully studied in population settings. The technology of metabolomics has enabled the simultaneous measurement of hundreds of small molecules in biological samples. Exploring the change of metabolites after feeding can not only help us understand the role of plant foods, but may also provide opportunities to assess plant food intake levels.

Official Title
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Dietary Intervention Studies on a Variety of Vegetables

Conditions
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Vegetable Signature Documentation With Metabolomic Technique

Intervention / Treatment
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* Other: Control
* Other: Spinach
* Other: Celery
* Other: Onion
* Other: Mixed Vegetables


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: BMI 18.5 30 kg/m2 non-smoker non-alcoholic Exclusion Criteria: Unwilling to stop taking the dietary supplements that may affect the experiment result. Unwilling to take the provided meal during the designated period. Taking hypertension or diabetes drugs. Diagnosed immune diseases (such as allergic diseases, autoimmune diseases, etc.), liver diseases, metabolic diseases (such as hyperthyroidism or hypothyroidism), or other not suitable for the dietary study. Diagnosed cancer or other severe diseases. Suffered from urinary tract infection or took antibiotic in the past three months

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 60 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Crossover Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Control<br>Participants were fed a standardized diet (without any vegetables) in this experiment. | Other: Control<br>* A standardized diet without any vegetables was provided.<br>|
| Experimental: Spinach<br>Participants were fed a standardized diet with 200g spinach in this experiment. | Other: Spinach<br>* A standardized diet with 200g spinach was provided.<br>|
| Experimental: Celery<br>Participants were fed a standardized diet with 200g celery in this experiment. | Other: Celery<br>* A standardized diet with 200g celery was provided.<br>|
| Experimental: Onion<br>Participants were fed a standardized diet with 200g onion in this experiment. | Other: Onion<br>* A standardized diet with 200g onion was provided.<br>|
| Experimental: Mixed Vegetables<br>Participants were fed a standardized diet with 200g of mixed vegetables (spinach, celery, and onion) in this experiment. | Other: Mixed Vegetables<br>* A standardized diet with 200g of mixed vegetables (spinach, celery, and onion) was provided.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | Baseline (fasting state before breakfast at 7:30AM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 4 Hours (immediately before lunch at 11:30AM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 5.5 hours (after lunch at 13:00PM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 6.5 hours (after lunch at 14:00 PM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 7.5 hours (after lunch at 15:00PM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 8.5 hours (after lunch at 16:00PM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 9.5 hours (after lunch at 17:00PM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 10.5 hours (after lunch at 18:00PM) | 
| Intensities of multiple serum metabolites | The outcome measure consists of intensities of multiple serum metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 11.5 hours (after lunch at 19:00PM) | 
| Intensities of multiple urinary metabolites | The outcome measure consists of intensities of multiple urinary metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | Baseline (overnight urine sample collected at 7:30AM) | 
| Intensities of multiple urinary metabolites | The outcome measure consists of intensities of multiple urinary metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 3.5 4 hours (before lunch urine sample collected during 11:00 11:30AM) | 
| Intensities of multiple urinary metabolites | The outcome measure consists of intensities of multiple urinary metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 4.5 6.5 hours (2-hour after lunch urine sample collected from 12:00 to 14:00PM) | 
| Intensities of multiple urinary metabolites | The outcome measure consists of intensities of multiple urinary metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 6.5 8.5 hours (4-hour after lunch urine sample collected from 14:00 to 16:00PM) | 
| Intensities of multiple urinary metabolites | The outcome measure consists of intensities of multiple urinary metabolites from a liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) experiment. Intensity means the level of response variable (a given metabolite) showing the degree of response detected by LC/TOF/MS. | 8.5 11.5 hours (7-hour after lunch urine sample collected from 16:00 to 19:00PM) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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vegetable metabolite signature, human serum/urinary metabolite, liquid chromatography mass spectrometry