Pathogenic Species and Genotypes of Bacterial Diversity in Relation to the Severity of Dental Caries

Study Overview
=================
Brief Summary
-----------------
The primary objective of this study is to estimate the distribution of pathogenic species and genotypes of bacterial diversity in 7 stages ICDAS (International Caries Detection and Assessment System) corresponding to the severity of dental caries among patients aged 3-45 years consulting in the Dentistry Departments of the Nîmes University Hospital and the Montpellier University Hospital.

Detailed Description
-----------------
Secondary objectives include: Compare the distribution of bacterial species in relation to stages of severity of caries Compare the results from plaque with those of saliva samples.

Official Title
-----------------
Prevalence and Identification of Pathogenic Species and Genotypes of Bacterial Diversity in Relation to the Severity of Dental Caries

Conditions
-----------------
Dental Cavity

Intervention / Treatment
-----------------
* Other: Dental cavity evaluation
* Biological: Saliva sampling
* Biological: Plaque sampling


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Volunteers aged 3 to 45 who come in to the hospital for a dental consultation Minors must have parental permission The patient must have given his/her informed and signed consent Exclusion Criteria: The patient is participating in another study The patient is in an exclusion period determined by a previous study The patient is pregnant, or breastfeeding The patient has been on antibiotics within the past two months Smokers Teeth under current care Parodontopathy Use of a mouthwash within the last week Insufficient numbers of healthy and/or teeth with carious lesions for carrying out the study

Ages Eligible for Study
-----------------
Minimum Age: 3 Years
Maximum Age: 45 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| 3 to 16 years of age<br>Volunteers of 3 to 16 years of age, coming in to the hospital for a dental consultation. See inclusion/exclusion criteria. Intervention: Dental cavity evaluation Intervention: Saliva sampling Intervention: Plaque sampling | Other: Dental cavity evaluation<br>* The dental cavities present will be evaluated using the ICDAS (International Caries Detection and Assessment System).<br>Biological: Saliva sampling<br>* A saliva sample will be harvested.<br>Biological: Plaque sampling<br>* Plaque samples from a healthy tooth and a tooth with a cavity will be harvested.<br>|
| 17 - 45 years of age<br>Volunteers of 17 to 45 years of age, coming in to the hospital for a dental consultation. See inclusion/exclusion criteria. Intervention: Dental cavity evaluation Intervention: Saliva sampling Intervention: Plaque sampling | Other: Dental cavity evaluation<br>* The dental cavities present will be evaluated using the ICDAS (International Caries Detection and Assessment System).<br>Biological: Saliva sampling<br>* A saliva sample will be harvested.<br>Biological: Plaque sampling<br>* Plaque samples from a healthy tooth and a tooth with a cavity will be harvested.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Bacterial profile | Bacterial profiles will be constructed using saliva and plaque samples. | Baseline (day 0) | 
| ICDAS assessment | Dental caries will be assessed using the ICDAS (International Caries Detection and Assessment System) system | Baseline (day 0) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Studies were stopped during high school? yes/no |  | baseline (Day 0) | 
| Does the patient have at least five fillings? yes/no |  | Baseline (day 0) | 
| Does the patient have at least one filling and one missing tooth on the anterior sector following the carious lesion? yes/no |  | baseline (day 0) | 
| Within the last 12 months, the patient has had dental care or tooth removal following the carious lesion? yes/no |  | baseline (day 0) | 
| Yesterday, did the patient eat at least three snacks (or sugary or carbonated drinks) between meals? yes/no |  | Baseline (day 0) | 
| Yesterday, did the patient brush his/her teeth fewer than 2 times? yes/no |  | baseline (day 0) | 
| The patient has at least one carious lesion restricted to the enamel? yes/no |  | baseline (day 0) | 
| The patient has at least one carious lesion restricted to the enamel on an anterior tooth or involving the smooth surface on a posterior tooth (yes/no) |  | baseline (day 0) | 
| The patient has at least one carious lesion attacking the dentin or pulp. yes/no |  | baseline (day 0) | 
| The patient has at least one carious lesion reaching dentin or pulp on an anterior tooth or on the smooth surface of a posterior tooth (yes/no) |  | baseline (day 0) | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
hygiene, Bacteria, microbial community