Safety and Tolerability of Single and Multiple Doses of ISIS 353512 in Healthy Volunteers

Study Overview
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Brief Summary
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The purpose of this study is to evaluate the safety and tolerability of single and multiple doses of ISIS 353512, to evaluate the blood concentration and rate elimination of ISIS 353512 from the blood, and to evaluate the effectiveness of ISIS 353512 in lowering a protein in the blood associated with inflammation.

Detailed Description
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This will be a Phase 1, double blind, placebo-controlled, dose-escalation study conducted at a single center to 1)evaluate the safety and tolerability of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously, 2)evaluate the pharmacokinetic profile of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously, and 3)evaluate the pharmacodynamics of ISIS 353512 in lowering constitutive levels of c-reactive proteins in healthy volunteers.

Official Title
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Double Blind, Placebo-Controlled, Dose-Escalation, Phase 1 Study to Assess the Safety, Tolerability, Pharmacokinetics and Pharmacodynamics of Single and Multiple Doses of ISIS 353512 Administered Intravenously and Subcutaneously to Healthy Volunteers

Conditions
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Inflammatory Diseases

Intervention / Treatment
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* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512
* Drug: ISIS 353512


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age 18 to 55 years Male or female although females must be post-menopausal or surgically sterile In good health BMI < 32 kg/m2 Give written informed consent to participate in the study hsCRP at Screening ≥ 0.50 mg/L and ≤ 5.0 mg/L (for 2 measurements ≥ 2 weeks apart; with difference between measurements ≤ 2.5 mg/L)(Multiple Dose Subjects only, not including dose-titration cohorts) Exclusion Criteria: Pregnant women, nursing mothers or women of childbearing potential Clinically significant abnormalities in medical history or physical examination Clinically significant ECG abnormalities or vital sign abnormalities (systolic blood pressure < 90 mm Hg or > 140 mm Hg, diastolic blood pressure < 50 mm Hg or > 90 mm Hg or heat rate < 50 or > 100 bpm) at Screening Clinically significant abnormalities on laboratory examination, other than hsCRP Clinically significant abnormalities in coagulation parameters Positive test for HIV, TB (PPD test) or hepatitis B or C at Screening History of latent or active tuberculosis or exposure to endemic areas within 8 weeks prior to PPD test at Screening History of positive PPD test or positive PPD test result (≥ 5 mm) indicating possible tuberculosis infection Fasting triglycerides > 400 mg/dL at Screening Malignancy (with the exception of basal or squamous cell carcinoma of the skin if adequately treated and no recurrence for > 1 year) Evidence of ongoing chronic inflammatory condition or infection Any other concurrent condition which, in the opinion of the Investigator, would preclude participation in this study or interfere with compliance Regular use of alcohol within 6 months prior to Screening (> 14 units of alcohol per week; 1 unit = 150 mL of wine, 360 mL of beer, or 45 mL of 40% alcohol) Use of soft drugs (such as marijuana) within 3 months prior to Screening or hard drugs (such as cocaine, phencyclidine [PCP] and crack) within 1 year prior to Screening, or positive urine drug screen at Screening Smoking > 10 cigarettes per day Use of an investigational drug or participation in an investigational study involving a small molecule with 30 days prior to dosing or participation in an investigational study involving biologic compounds within 90 days prior to dosing Regularly taking OTC medications and/or vitamin supplements that may alter CRP, e.g., NSAIDs, aspirin, vitamin E, fish oil, within 14 days prior to Screening Receiving prescription medications, including hormone replacement therapy, statins, TNF-α inhibitors, or other anti-inflammatory or immunosuppressive drugs within 30 days prior to Screening Immunization with a live attenuated vaccine one month prior to dosing or planned vaccination during the course of the study Blood donation of 50 to 499 mL within 30 days of Screening or of > 499 mL within 60 days of Screening

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 55 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Single Group Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: A<br>single-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 355312 or placebo | Drug: ISIS 353512<br>* 50 mg via 2 hour IV infusion, single dose<br>|
| Experimental: B<br>single-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 100 mg via 2 hour IV infusion, single dose<br>|
| Experimental: C<br>single-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 200 mg via 2 hour IV infusion, single dose<br>|
| Experimental: AA<br>multiple-dose cohort (n=4, randomized 3 active : 1 placebo) receiving 353512 or placebo | Drug: ISIS 353512<br>* 50 mg via 2 hour IV infusion, 6 doses over 22 days<br>|
| Experimental: BB<br>multiple-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 100 mg via 2 hour IV infusion, 6 doses over 22 days<br>|
| Experimental: CC<br>multiple-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 200 mg via 2 hour IV infusion, 6 doses over 22 days<br>|
| Experimental: G<br>single-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 50 mg via SC injection, single dose<br>|
| Experimental: H<br>single-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 100 mg via SC injection, single dose<br>|
| Experimental: I<br>single-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 200 mg via SC injection, single-dose<br>|
| Experimental: GG<br>multiple-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 50 mg via SC injection, 6 doses over 22 days<br>|
| Experimental: HH<br>multiple-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 100 mg via SC injection, 6 doses over 22 days<br>|
| Experimental: II<br>multiple-dose cohort (n=4, randomized 3 active : 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 200 mg via SC injection, 6 doses over 22 days<br>|
| Experimental: F (100 mg)<br>single-dose cohort (n=4, randomized 3 active: 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 100 mg via SC injection PLUS an oral treatment of a NSAID, acetaminophen, or oral placebo<br>|
| Experimental: Dose-Titration 1<br>multiple-dose cohort (n=4, randomized 3 active: 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 2 hour IV infusion, 50 mg on day 3, 100 mg on day 5, and 200 mg on day 8<br>|
| Experimental: F (200 mg)<br>single-dose cohort (n=4, randomized 3 active: 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 200 mg via SC injection PLUS an oral treatment of a NSAID, acetaminophen, or steroid<br>|
| Experimental: Dose-Titration 7<br>multiple-dose cohort (n=4, randomized 3 active: 1 placebo) receiving ISIS 353512 or placebo | Drug: ISIS 353512<br>* 2 hour IV infusion, 50 mg on day 1, 100 mg on day 3, 100 mg on day 5 and 200mg on day 8<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To evaluate the safety and tolerability of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously. |  | 14 Days post treatment of each cohort | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| To evaluate the pharmacokinetic profile of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously. |  | 14 Days post treatment of each cohort | 
| To evaluate the pharmacodynamics of ISIS 353512 administered intravenously and subcutaneously. |  | 14 Days post treatment of each cohort |