Effect of Tomato Extracted Lycopene on Postprandial Oxidation and inﬂammation in Healthy Weight Men and Women

Study Overview
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Brief Summary
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The hypothesis of the study is that tomato extracted lycopene will be able to decrease postprandial oxidation and inﬂammation in healthy weight men and women when compared to Placebo.

Detailed Description
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Consumption of a high-fat meal results in a postprandial (fed-state) response characterized by hypertriglyceridemia. Postprandial hypertriglyceridemia increases the oxidation of low-density lipoproteins (LDL) and increases blood concentrations of several biomarkers of inﬂammation. This postprandial lipemia-induced oxidative stress mediated response to a high-fat meal has been suggested as a major contributor to the pathogenesis of atherosclerosis along with other chronic disease states of diabetes and obesity. Consumption of foods rich in antioxidant compounds provides a defence source to compliment endogenous defence systems to protect against oxidative damage during pro-oxidant conditions.

Official Title
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Effect of Tomato Extracted Lycopene on Postprandial Oxidation and inﬂammation in Healthy

Conditions
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Postprandial Oxidation and inﬂammation

Intervention / Treatment
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* Dietary Supplement: Tomato extracted lycopene
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male or female Age: 21 - 70 years both inclusive Subject with Body Mass Index: 19- 25 kg/m2 both inclusive Subject with Fasting serum LDL-cholesterol: ≥ 100 mg/dL and < 200 mg/dL Subject with High-sensitivity C-reactive protein (hsCRP) < 1.0 mg/L Subject is willing to take supplement once daily for 4 weeks and undergo other study-related procedures Subject is otherwise is in general good health as determined by the principal investigator Subjects is willing to sign an informed consent form prior to joining the study Exclusion Criteria: Subjects suffering from overweight defined as BMI > 25 kg/m2 Subject that is actively losing weight (e.g. are on a diet) or subjects with greater than 5% change in body weight within 1 month prior to the randomization visit Subject taking lipid-altering drug therapy within six weeks prior to screening. Also excluded are supplements known to have significant lipid altering effects, such as niacin, garlic, omega-3 fatty acids, red yeast rice extract, phytostanols / phytosterols, soluble fiber, chitosan and conjugated linoleic acid Subjects using the following medications: systemic corticosteroids, orlistat, bile acid resins, prescription omega-3 fatty acids, cyclical or non-continuous hormone therapy Subjects that use antioxidant agents or vitamins within 6 weeks prior to inclusion into the study. For subject using vitamin supplementation a 6 week wash out will be required prior to inclusion into the study. Subjects that will not be able to follow dietary proscriptions from the screening visit through the final visit Subjects following any special diet including, but not limited to liquid, high or low protein, raw food, vegetarian or vegan, etc. Subjects with known allergy to tomato, carotenoids, or vitamin E Subjects that smoke (past smokers are allowed if smoking was ceased > 2 years prior to study inclusion) Subjects that has high fasting serum triglyceride Subjects that has a diagnosis of type 1 or type 2 diabetes mellitus Subjects that has high serum thyroid-stimulating hormone Subjects that has aspartate aminotransferase (AST) or alanine transaminase (ALT) > 3 times the upper limit of normal Subjects that has Creatinine ≥ 1.5 mg/dl Subjects that has high-sensitivity C-reactive protein Woman subjects with positive pregnancy test Woman subjects that are breast feeding, pregnant, or planning on becoming pregnant during the duration of the study Subjects with known cardiovascular disease or stroke, except for conditions that are deemed clinically insignificant by Principle Investigator or Sub-investigator, or study site physician (e.g. clinically insignificant atherosclerotic lesions observed by imaging studies). Subject with history of significant gastrointestinal disease such as severe constipation, diarrhea, malabsorptive disease, inflammatory bowel disease (e.g. Crohn's disease, ulcerative colitis) Subject with history of severe psychiatric illness which in the opinion of the investigator would interfere with the optimal participation in the study Subject with history of bariatric surgery Subject with history or current use of illegal or recreational drugs Subject that used any investigational drug(s) 60 days prior to screening Subject that participate in any other clinical trial while participating in this trial

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 70 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: Placebo<br>Soft gel capsule without test material | Dietary Supplement: Placebo<br> <br> |
| Experimental: Tomato extracted lycopene<br>Soft gel cups for oral use | Dietary Supplement: Tomato extracted lycopene<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Reduction of postprandial Oxidized LDL(OX-LDL) levels following 2 weeks of supplementation with tomato extracted lycopene | Following two weeks of supplementation the evaluation will be done at baseline,before the high fat meal and during 6 hours following the meal. | Baseline and over 6 hours | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Reduction of postprandial triglycerides, glucose, insulin and hsCRP levels following 2 weeks of supplementation with tomato extracted lycopene | Following two weeks of supplementation the evaluation will be done at baseline,before the high fat meal and during 6 hours following the meal. | Baseline and over 6 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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anti oxidation, lycopene, Ox-LDL