Effect of Acute Red Raspberry Consumption on Post-prandial Oxidative Stress

Study Overview
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Brief Summary
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Eight healthy adult subjects will be given a meal of one, two or four cups red raspberries, or two slices white bread with or without 200 mg vitamin C, after an overnight fast and consumption of a low polyphenol diet for two days. Blood samples will be taken at various time points over an eight hour period to measure oxidative stress and antioxidant levels in the blood. Each subject will attend 5 study visits with one week intervals and be given each meal in a random order. It is hypothesized that raspberry consumption at higher doses will greater protect against meal-induced oxidative stress compared to bread controls.

Official Title
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An Acute Intervention Study Investigating the Effect of Red Raspberry Consumption on Post-prandial Oxidative Stress

Conditions
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Oxidative Stress, Antioxidants

Intervention / Treatment
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* Dietary Supplement: red raspberries
* Dietary Supplement: bread controls


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy adults Exclusion Criteria: blood pressure greater than 120/80 mm Hg body mass index greater than 24.9 kg/m^2 history of any chronic disease currently taking medications allergy to fruit

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: one cup red raspberries<br>treatment meal consists of one cup red raspberries | Dietary Supplement: red raspberries<br>* single serving of one, two or four cups red raspberries<br>|
| Experimental: two cups red raspberries<br>treatment meal consists of two cups red raspberries | Dietary Supplement: red raspberries<br>* single serving of one, two or four cups red raspberries<br>|
| Experimental: four cups red raspberries<br>treatment meal consists of four cups red raspberries | Dietary Supplement: red raspberries<br>* single serving of one, two or four cups red raspberries<br>|
| Placebo Comparator: bread<br>treatment meal consists of two slices white bread | Dietary Supplement: bread controls<br>* two slices white bread alone or with 200 mg vitamin C supplement<br>|
| Active Comparator: vitamin C<br>treatment meal consists of two slices white bread and 200 mg vitamin C in the form of supplemental ascorbic acid | Dietary Supplement: bread controls<br>* two slices white bread alone or with 200 mg vitamin C supplement<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in antioxidant capacity from baseline (-0.25 h) | plasma antioxidant capacity measured using the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation assay and expressed in Trolox Equivalents (TE) | -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| change in lipid peroxidation from baseline (-0.25 h) | serum thiobarbituric acid reactive substances (TBARS) measured as an indicator of the lipid peroxidation product malondialdehyde (MDA) | -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake | 
| change in thiols compared to baseline (-0.25 h) | plasma thiols quantified by 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) assay as an indicator of the level of protection against protein oxidation | -025, 0.25, 0.5, 1, 2, 4 hours post-intake | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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red raspberries, post-prandial, oxidative stress, antioxidants, polyphenols, vitamin C, acute dietary intervention