Relationship Between ALDH2 and CIN

Study Overview
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Brief Summary
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The purpose of this clinical trial is to investigate the relationship between the gene polymorphism of aldehyde dehydrogenase 2 and contrast induced nephropathy and its mechanism.

Detailed Description
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Patients undergoing coronary angiography or percutaneous coronary intervention will be recruited in this study after they sign the informed consent.5ml venous blood was extracted from the peripheral vein before the operation to detect the renal function, the genotype and enzyme activity of aldehyde dehydrogenase 2 ,and the levels of oxidative stress and inflammation.Another 5ml venous blood was extracted from the peripheral vein 24-72 hours after the operation to detect the renal function and the levels of oxidative stress and inflammation.Activated oxygen protein products , 4-HNE and hs-CRP as markers for oxidative stress and inflammation.The renal function index contains serum creatinine，urea nitrogen and cystatin C.

Official Title
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Relationship Between the Gene Polymorphism of Aldehyde Dehydrogenase 2 and Contrast Induced Nephropathy

Conditions
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Radiographic Contrast Agent Nephropathy

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients undergoing elective coronary intervention or coronary angiography at Yu Qilu hospital. All the patients or their guardians must sign the consent form before entering the trial. Exclusion Criteria: Patients with acute renal failure, renal transplantation, end-stage renal disease, or dialysis treatment, GFR < 15ml/min. Use of renal toxicity drugs during the treatment, such as non steroidal anti-inflammatory drugs, etc. Patients who has used contrast agent 10 days before the operation. Cardiac shock, hypotension (systolic blood pressure < 95mmHg),or hyper- tension with difficulty to control. Allergy to contrast media. Congestive heart failure (left ventricular ejection fraction<40%), cardiac function NYHA grade IV. Pregnancy or lactation.

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| CIN<br>CIN was defined as an absolute increase in serum creatinine concentration of at least 0.5 mg/dL (44.2umol/l) or a relative rise of at least 25% from baseline on the follow-up blood sample drawn 24 to 72 hours after the operation. | |
| Control<br>The enrolled patients without CIN. | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Number of patients with different ALDH2 genotype |  | 1-30 days after samples collected | 
| Change from baseline renal function after the operation | The baseline of renal function will be tested at the next morning after admission .The renal function will be tested again 24-72 hours after coronary angiography or coronary intervention.The changes of renal function will be recorded.The renal function index contains serum creatinine,urea nitrogen and cystatin C. | at admission and 24-72hours after coronary angiography or coronary intervention | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Aldehyde dehydrogenase 2 activity |  | 1-30 days after samples collected | 
| Activated oxygen protein products, 4-HNE and hs-CRP | Activated oxygen protein products , 4-HNE and hs-CRP as markers for oxidative stress and inflammation. | 1-30 days after samples collected | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Aldehyde dehydrogenase 2, contrast induced nephropathy, Gene polymorphism