New Transporting Solution in Parathyroid Allotransplantation

Study Overview
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Brief Summary
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The aim of this study is to measure of the parathyroid cell viability and the the amount of calcium sensing receptor of the parathyroid cells which were placed into a new transportation solution (BAVU solution) for parathyroid allotransplantation.

Detailed Description
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The cold ischemia of organs, has harmful effects on the tissues due to oxidative stress and inflammation. This is physiopathologically defined as ischaemia/re-perfusion injury (IRI) in organ transplantation. Parathyroid allotransplantation has an increased popularity at the last decade. But the cold ischemia due to transportation of the transplant tissue is a very important problem. Calcium sensing receptor plays a crucial role in regulating Parathormone secretion which means controlling of the calcium-dependent systemic ion homeostasis. The investigators developed a new tissue transporting solution for parathyroid allotransplantation and the investigators measured the parathyroid cell viability and the the amount of calcium sensing receptor of the parathyroid cells at the 0., 6., 12. and 24. hours of the parathyroid cells which were placed into our new solution.

Official Title
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The Effects of Cold Ischemia Time With a New Transporting Solution to Parathyroid Cell Viability and Calcium Sensing Receptor in Parathyroid Allotransplantation

Conditions
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Parathyroid Allotransplantation

Intervention / Treatment
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* Drug: BAVU Solution


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients who have parathyroid hyperplasia due to chronic renal failure Exclusion Criteria: Patients who refused to join to the study Patients younger than 18 years and older than 80 years. Patients who have hepatitis B, Patients who have hepatitis C, Patients who have human immunodeficiency virus (HIV)

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 80 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| No Intervention: BAVU Solution 0. hour (control)<br>Viability, Calcium sensing receptor and parathormone levels of Parathyroid cells at 0. hour | |
| Experimental: BAVU Solution 6. hour<br>Viability, Calcium sensing receptor and parathormone levels of Parathyroid cells at 6. hour | Drug: BAVU Solution<br>* Tissue preservation solution for transplantation<br>|
| Experimental: BAVU Solution 12. hour<br>Viability, Calcium sensing receptor and parathormone levels of Parathyroid cells at 12. hour | Drug: BAVU Solution<br>* Tissue preservation solution for transplantation<br>|
| Experimental: BAVU Solution 18. hour<br>Viability, Calcium sensing receptor and parathormone levels of Parathyroid cells at 18. hour | Drug: BAVU Solution<br>* Tissue preservation solution for transplantation<br>|
| Experimental: BAVU Solution 24. hour<br>Viability, Calcium sensing receptor and parathormone levels of Parathyroid cells at 24. hour | Drug: BAVU Solution<br>* Tissue preservation solution for transplantation<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Cell viability of Parathyroid cells | The investigators will measure the viability of Parathyroid cells at 0 hour at the BAVU solution | 0 hour | 
| Cell viability of Parathyroid cells | The investigators will measure the viability of Parathyroid cells at 6 hours at the BAVU solution | 6 hours | 
| Cell viability of Parathyroid cells | The investigators will measure the viability of Parathyroid cells at 12 hours at the BAVU solution | 12 hours | 
| Cell viability of Parathyroid cells | The investigators will measure the viability of Parathyroid cells at 18 hours at the BAVU solution | 18 hours | 
| Cell viability of Parathyroid cells | The investigators will measure the viability of Parathyroid cells at 24 hours at the BAVU solution | 24 hours | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Calcium sensing receptor, Parathyroid cell viability, Parathyroid allotransplantation, Transporting solution