Assesment of Salivary Alpha-amylase Levels Before and After Ovulation

Study Overview
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Brief Summary
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Investigators aimed to investigate whether there is an association between ovulation and salivatory alpha amylase (sAA) activity in menstrual cycles during a month period in a group of reproductive women.

Detailed Description
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Saliva is a readily available specimen, which can be collected by noninvasive procedures and contains many hormones, drugs and antibodies of interest in screening and diagnosis. With a salivary specimen, one can collect multiple specimens at the optimum times for diagnostic information. Saliva can be collected in remote sites by unskilled personnel and, is stable at ambient temperatures for several weeks. Because of diurnal and monthly variations, several steroid hormones need multiple samples collected early in the morning or late at night or at the same time every day for a month to give meaningful results. Such collections are often very expensive, inconvenient or impossible to do with blood. Investigators aimed to investigate whether there is an association between ovulation and salivatory alpha amylase (sAA) activity in menstrual cycles during a month period in a group of reproductive women.

Official Title
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Assesment of Salivary Alpha-amylase Levels Before and After Ovulation: A Randomized Controlled Trial

Conditions
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Salivary Amylase

Intervention / Treatment
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* Other: Salivary alpha-Amylases


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Clinical diagnosis of PCOS (study group) Reproductive age regular menses women Exclusion Criteria: pregnancy, breastfeeding, known liver disease, alcohol abuse, diabetes mellitus, smoking treatment with oral glucocorticoids treatment with hormonal contraceptives.

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| study group<br>Reproductive age women with polycystic ovary syndrome and irregular menses (Salivary Alpha-amylase Levels of 3rd and 24th day of menstrual cyclus) | Other: Salivary alpha-Amylases<br>* Investigators will collect Salivary alpha-Amylases before and after ovulation<br>|
| control group<br>Reproductive age women with regular menses (Salivary Alpha-amylase level of 3rd and 24th day of menstrual cyclus) | Other: Salivary alpha-Amylases<br>* Investigators will collect Salivary alpha-Amylases before and after ovulation<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Changes of Salivary Alpha-amylase activity | Saliva samples will be taken 2 times during the menstrual cyclus (3th day and 24th day). | 3rd and 24th day of menstrual cyclus | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Salivary Alpha-amylase, ovulation