Skeletal Muscle Energy Metabolism in Women With Weight Loss and Ovarian and/or Endometrial Cancer With Weight Loss

Study Overview
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Brief Summary
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the aim is to study skeletal muscle metabolism alterations métaboliques associated with weight loss in women with ovarian and/or endometrial cancer according to BMI.

Official Title
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Study of Differences in Skeletal Muscle Energy Metabolism Alterations in Women With Weight Loss and Ovarian and/or Endometrial Cancer Based on the Body Mass Index

Conditions
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Weight Loss, Ovarian Carcinoma, Endometrial Carcinoma

Intervention / Treatment
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* Other: Samples


Participation Criteria
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Eligibility Criteria
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Case group selection criteria: Inclusion Criteria: Women aged 18 years old and more Ovarian and/or endometrial cancer weight loss related to cancer (weight loss > 5% weight in one month or 10% in 6 months) Indication to therapeutic surgery Women affiliated to social security scheme Women who can understand French and able to sign Informed consent Exclusion Criteria: Unbalanced Diabetes Chronic neuromuscular disorder Any severe uncontrolled medical condition Control group selection criteria: Inclusion Criteria: Women aged 18 years and more Surgery Indication for a benign endometrial and/ or ovarian disease Patient affiliated to social security scheme Women who can understand French and able to sign Informed consent Exclusion Criteria: Unbalanced Diabetes Chronic neuromuscular disorder Any severe uncontrolled medical condition

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Health Services Research
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Case group<br>women with ovarian and/or endometrial cancer and weight loss will have muscle, adipose tissue, ovarian tumor and blood samples | Other: Samples<br>* Blood samples, muscle biopsy, adipose tissue samples and ovarian cancer tumor sample<br>|
| Other: Control group<br>women scheduled for an intervention for benign ovarian/ endometrial disease and no weight loss will have muscle, adipose tissue and blood samples | Other: Samples<br>* Blood samples, muscle biopsy, adipose tissue samples and ovarian cancer tumor sample<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Measurement of muscle mitochondrial bioenergetics according to BMI | Measurement of muscle mitochondrial bioenergetics by high resolution oxygraphy | muscle biopsy realized during surgery | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| on muscle biopsy | Quantification of lipids on electron microscope Type of muscular fibers Analyze of gene expression and proteins involved in 1/ Mitochondrial metabolism 2/ lipid metabolism and 3/ muscular proteolysis by RTqPCR with SyBR Green and Western Blot | muscle biopsy realized during surgery | 
| on white adipose tissue | adipose tissue composition (fatty acids quantification, sterols, steroids) RT-qPCR quantification of molecules involved in lipid metabolism (synthesis, transport) and in inflammation (immune profile and cytokines) RTqPCR analysis, western blot and immunohistochemical expression of markers of transformation into brown adipose tissue in specific milieu, in vitro experimentation on skeletal muscular cells | adipose tissue samples taken during surgery | 
| on ovarian cancer tumor samples | lipid composition (fatty acids quantification, sterols, steroids) RT-qPCR quantification of molecules involved in lipid metabolism (synthesis, transport) and in inflammation (immune profil and cytokines) culture of tumour cells to evaluate resistance to chemotherapy (platinum) | ovarian tumor biopsies realized during surgery | 
| on computerized tomography | assessment of body composition (fat and muscle compartments) | 1 day of abdominal CT scan imaging procedure performed at diagnosis | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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weight loss, ovarian cancer, endometrial cancer, energy metabolism, Body mass index, obesity, muscle energy