Biological Aging of Skeletal Muscles in Humans

Study Overview
=================
Brief Summary
-----------------
Aging affects almost all the tissues and physiological functions, and skeletal muscle is the most affected organ. The progressive decline of the weight and the muscular function linked to the aging contributes to the lack of autonomy and dependence, but also to an increase of the mortality risks. Sarcopenia is also a prevalent condition, because it is detected in 13-24% of 60 years old, and 50% of 80 years old and more. However, strong inter-individual variations of this prevalence of sarcopenia exists. The key issue is to understand why the biological aging of the skeletal muscle is so different between people. In this study, mechanisms involved in biological aging of the skeletal muscle in aging people (same chronological age) will be specified.

Detailed Description
-----------------
Aging affects almost all the tissues and physiological functions, and skeletal muscle is the most affected organ. The progressive decline of the weight and the muscular function linked to the aging contributes to the lack of autonomy and dependence, but also to an increase of the mortality risks. Sarcopenia is also a prevalent condition, because it is detected in 13-24% of 60 years old, and 50% of 80 years old and more. However, strong inter-individual variations of this prevalence of sarcopenia exists. Some elderly (60 years old) reveal a biological aging of 80 years old, whereas 60 years old people reveal a biological aging of 60 years old. The key issue is to understand why the biological aging of the skeletal muscle is so different between people. Previous sarcopenia studies in Humans did not really focus on chronological aging, they were all based on a comparison between young and old people. No study considered inter-individual modifications (biological aging) in sarcopenia. Furthermore, few studies were associated in the same study to omic, histological, and epigenetic data, to obtain integrated point of view of Human Sarcopenia. In this study, mechanisms involved in biological aging of the skeletal muscle in aging people (same chronological age) will be specified.

Official Title
-----------------
Biological Aging of Skeletal Muscles in Humans, a Monocentric, Prospective Study

Conditions
-----------------
HEALTHY

Intervention / Treatment
-----------------
* Other: Proof cohort : muscular assessment


Participation Criteria
=================
Eligibility Criteria
-----------------
Inclusion Criteria: Belonging to the Proof cohort Between 80 and 83 years old Informed consent signed Genetic analyses consent signed Subject affiliated or entitled to a social security scheme Exclusion Criteria: Anti coagulative treatment Severe obesity (>35) All chronic pathology requiring a treatment Severe renal insufficiency (discovery less than 6 months) Abnormal coagulation appraisal Allergy to local anesthetics Installation of a prosthetic hip and / or knee in the last six months Senile dementia Refusal of genetic study

Ages Eligible for Study
-----------------
Minimum Age: 80 Years
Maximum Age: 83 Years

Sexes Eligible for Study
-----------------
All

Accepts Healthy Volunteers
-----------------
Accepts Healthy Volunteers

Study Plan
=================
How is the study designed?
-----------------

Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Proof cohort : muscular assessment<br>Clinical examination : Body weight, BMI, cardiac frequency, muscular examination,... Blood appraisal : glycaemia, lipidic appraisal, leptin, albumin, coagulation, metabolome and epigenetic tests,... Urinary collection : metabolome tests Maximal voluntary quadriceps strength (MVC) Checking muscle functional skills Muscular biopsy | Other: Proof cohort : muscular assessment<br>* Clinical examination, blood appraisal, urinary collection, MVC, checking muscle functional skills and muscular biopsy will be performed.<br>|

What is the study measuring?
-----------------
Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The area of type IIA fibers called vastus lateralis. | The primary endpoint is the cross sectional area (measured in µm2) of type IIA fibers from the vastus lateralis muscle biopsies. | Day 15 | 

 Terms related to the study
=================
Keywords Provided by Centre Hospitalier Valida
-----------------
sarcopenia, elderly, muscle biopsy