Impact of 5-Fluorouracil (5-FU) on Cancer Cells From Liver Metastases of Colon Cancer

Study Overview
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Brief Summary
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Analyze the dynamics of incorporation of 5-fluorouracil into cancer cell ribosomes from liver metastases of patient with metastatic colon cancer.

Detailed Description
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This is a monocentric, prospective, non-interventional cohort study in adult patients with metastatic colon cancer with hepatics metastasis. Tumour samples collected for this study include: A fragment of the previously operated primary tumor: FFIP block and/or archived frozen tumor if available. A fragment of the liver metastasis(s) performed as part of standard metastasis surgery, this fragment will include a tumor area and a healthy area within the margin of excision. These samples collected in a fresh state will be analyzed immediately.

Official Title
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Analysis of the Impact of 5-Fluorouracil on the Translational Control and Plasticity of Cancer Cells From Liver Metastases of Colon Cancer

Conditions
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Cancer of Colon, Metastasis to Liver

Intervention / Treatment
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* Procedure: Scheduled and planned surgery


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patient with metastatic colon cancer operated on and followed up at the Centre Leon Berard for liver metastasis. Patient affiliated to social security Exclusion Criteria: Other pathologies that may interfere with biological evaluation, in particular known hepatic cirrhosis, whatever its origin, other liver overload diseases (hemochromatosis, Wilson's disease), but excluding steatosis (which can be chemo-induced).

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Metastatic colon cancer<br>Patient with metastatic colon cancer operated on and followed up at the Centre Leon Berard for liver metastasis. | Procedure: Scheduled and planned surgery<br>* Collection of tumour samples from samples collected as part of standard patient management. Diagnostic pathological analysis will be a priority. These samples will be taken by experienced staff during the hospital visit scheduled as part of standard care.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| 5-FU in ribosome | Rate of incorporation of 5-fluorouracil into ribosomes of tumor tissues and adjacent healthy tissues before surgery and after surgery. | Up to 24 months | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Methylation profile of ribosomal RNAs | Analyze on a large scale the alterations of the 2'-O-methylation profile of ribosomal RNAs before surgery and after surgery | Up to 24 months | 
| Transcriptomic and proteomic profile | Define a transcriptomic and proteomic profile of the collected samples before surgery and after surgery. | Up to 24 months | 
| Specific molecular signature | Combination of the number of 5-fluorouracil molecules incorporated per rRNA molecule, the methylation profile and the transcriptomic profile before surgery and after surgery. | Up to 24 months |