Assessment of Glypican 3 as Apredictive Marker in Colorectal Cancer Patients

Study Overview
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Brief Summary
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Evaluation Of Glypican 3 in serum of colorectal cancer patients . Correlation between Glypican 3 and clinicopathological characteristics of colorectal cancer .

Detailed Description
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Colorectal cancer (CRC) is the third most common malignancy and the fourth most prominent cause of cancer-related deaths worldwide. Also it is the 7th commonest cancer in Egypt, representing 3.47% of male cancers and 3% of female cancers.The Glypicans , a family of proteins classified as HSPGs (heparan sulfate proteoglycan) , have been shown to interact with a number of growth factors and modulate growth factor activity and are linked to the xtracellular side of cell membrane by a glycosylphosphatidylinositol (GPI) anchor . Members of this large family of transmembrane proteins have been identified in both mammals and drosophila: 6 glypicans (GPC-1 through GPC-6) in mammals, and two others in the fly .Glypican-3 (GPC3) as one of this family is a protein of about 70 kD . It has multiple sugar chains and heparan sulfate modification sites . These proteins (Glypicans) participate in organ development by modulating extracellular growth signals and morphogen gradient formation, and are involved in human overgrowth and skeletal dysplasia problems .In some cancers, they are highly expressed, associated with tumorigenesis, and regulating angiogenesis for cancer progression and invasion . Abnormal expression of glypicans has been noted in multiple types of cancer. For examples, GPC-1 expression was found to be increased in breast cancer tissues and ovarian malignant tumors .GPC-2 is associated with neuroblastoma .Expression of GPC-3 was found in high-grade urothelial carcinoma and also reported in some non-CNS tumors of the brain.There have been several reports about the clinical usefulness of the N-terminal form of GPC3. Enzyme-linked immunosorbent assay methods capable of detecting the N-terminal form of GPC3 . A study of colorectal cancer tissue speciment shows a correlation between expression of GPC3 with the clinicopathologic variables such as the level of differentiation, GPC3 was found to be expressed in ( 42.8% ) of the well-differentiated tumors, and (50%) of the moderately differentiated tumors, (75%) of the moderately and poorly differentiated tumors .Therefore, it was found that the expression of GPC3 was correlated with the pathological grade of the tumors .

Official Title
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Assessment of Glypican 3 as a Predictive Marker in Colorectal Cancer Patients

Conditions
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Colorectal Cancer

Intervention / Treatment
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* Diagnostic Test: glypican 3


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Patients with colorectal cancer and admitted to south egypt cancer institute . Exclusion Criteria: Other malignant diseases . Patients received chemotherapy for colorectal cancer .
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Diagnostic
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Single


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: patients with colorecta cancer<br> | Diagnostic Test: glypican 3<br>* Assessment of glypican 3 presence in colorectal cancer patients using ELISA<br>|
| No Intervention: healthy individuals<br> | |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Evaluation of Glypican 3 role as a predictive and diagnostic marker of colorectal marker . | measurment of GLYPICAN 3 level in serum of colorectal patients using ELISA method | 3 years | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Addition of Glypican 3 to the routine markers of colorectal cancer . - Correlatin of this marker with the disease pathology and management procedures . | measurment of GLYPICAN 3 level in serum of colorectal patients using ELISA method | 3 years | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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glypican 3