Atrificial Shrinkage of Blastocyst Before In Vitro Fertilization

Study Overview
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Brief Summary
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Vitrification is a modern cryopreservation technique based on sudden cooling of reproductive cells to a temperature of -196 ° C in seconds that produces glass-like solidification of a solution without crystallization, but by extreme elevation in viscosity during freezing

Detailed Description
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Vitrification is a modern cryopreservation technique based on sudden cooling of reproductive cells to a temperature of -196 ° C in seconds that produces glass-like solidification of a solution without crystallization, but by extreme elevation in viscosity during freezing Unlike the slow freezing method, vitrification has better results in a complete elimination of ice crystal inside the cells as well as in the surrounding solution -Also, it has a significantly higher post-thawing survival rate in comparison to slow freezing method

Official Title
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Comparative Study Between Two Techniques for Artificial Shrinkage of Blastocysts Prior to Vitrification: LASER Pulse Versus Micro-needle Technique in Increasing Chemical, Clinical Pregnancy and Live Birth Rates After ICSI

Conditions
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Infertility

Intervention / Treatment
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* Device: ICSI with laser pulse
* Device: ICSI with micro needle technique


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: women with infertility from 20 to 44 years Exclusion Criteria: women with age more than 44 or less than 20

Ages Eligible for Study
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Minimum Age: 20 Years
Maximum Age: 44 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Supportive Care
Allocation: Randomized
Intervention Model: Parallel Assignment
Interventional Model Description: effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts .
Masking: Quadruple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Other: effect of using LASER pulse<br>effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability | Device: ICSI with laser pulse<br>* Using laser pulse before ICSI<br>Device: ICSI with micro needle technique<br>* Using micro needle technique before ICSI<br>|
| Other: Micro-needle Technique<br>effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability | Device: ICSI with laser pulse<br>* Using laser pulse before ICSI<br>Device: ICSI with micro needle technique<br>* Using micro needle technique before ICSI<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| The number of women who will have positive pregnancy test | The number of women who will come pregnant after each technique | 2 months |