Polygenic Risk Score to Predict Weight Loss Intervention in Children With Obesity

Study Overview
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Brief Summary
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Children with obesity are prone to suffering from metabolic diseases, which undoubtedly increases the burden of public health. Since obesity is a multiple gene disease, a comprehensive approach using polygenic risk scores (PRS), rather than individual genetic variant, may be a more appropriate method. The aim of the study was to establish a polygenic risk score model to assess differences to assess differences in weight loss treatment outcomes.

Detailed Description
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The investigators hypothesize that obesity gene variants can predict the efficacy of weight loss intervention in obese children. The aim of the study was to establish a polygenic risk score model to assess differences to assess differences in weight loss treatment outcomes. The investigators will also analyze whether these gene variants have an effect on obesity comorbidities (hypertension, hyperlipidemia, non-alcoholic fatty liver disease, type 2 diabetes, obstructive sleep apnea, polycystic ovary syndrome, etc.). For participants with non-simple obesity, the investigators will collect their complete family history, and perform whole exome sequencing to identify possible rare disease-causing genes. The experimental design is as follows: Obese children and adolescent subjects will undergo a 6-month weight loss intervention program and be followed for 12-18 months. The investigators will analyze obesity and fatty liver-related genes in these adolescents using next-generation gene sequencing and/or gene chips, perform polygenic risk score analysis, and use an additive model to total the number of variant loci weighted by effect size. Whole exome gene sequencing refers to the human DNA map (hg19), and Sanger sequencing will be used to confirm the correctness of the variant site.

Official Title
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Development of Polygenic Risk Score to Predict the Efficacy of Weight Loss Intervention in Children and Adolescents With Obesity

Conditions
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Obesity, Children

Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Age <18 years old Obesity definition: BMI > 95% according to the age- and gender-specific standard by National Health Institute in Taiwan Willing to give written informed consent Exclusion Criteria: Alcohol consumption Major systemic diseases, including cardiopulmonary disease, renal failure, cancer, and major psychotic disorder

Ages Eligible for Study
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Maximum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| weight loss | changes of weight and/or BMI z score | 6 month | 
| obesity severity | BMI z score/BMI percentile | 1 month | 
| fatty liver | quantification by liver ultrasound/Fibroscan | 1 month | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| hyperlipidemia | including triglyceride, HDL cholesterol, total cholesterol | 1 month | 
| hypertension | systolic and diastolic blood pressure | 1 month | 
| fasting glucose | hyperglycemia | 1 month | 
| HbA1c | hyperglycemia | 1 month | 
| 2 hours glucose tolerance test | hyperglycemia | 1 month | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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whole exome sequencing, polygenic risk score, weight loss