The Effect of ProHydrolase® on the Amino Acid, Intramuscular Anabolic Signaling, and Endocrine Response to Resistance Exercise in Trained Males

Study Overview
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Brief Summary
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To examine the amino acid absorption following acute resistance exercise between three supplemental treatments: 1) Whey Protein + ProHydrolase (WPH) 2) Whey Protein (W) and 3) Non-Caloric Placebo (PL). To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on the mTORC1 complex pathway. To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on circulating concentrations of endocrine biomarkers.

Official Title
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The Effect of ProHydrolase® on the Amino Acid, Intramuscular Anabolic Signaling, and Endocrine Response to Resistance Exercise in Trained Males

Conditions
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Amino Acids

Intervention / Treatment
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* Dietary Supplement: ProHydrolase®
* Dietary Supplement: Whey protein
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: • At least one-year of resistance training experience Free of any physical limitations (determined by health and activity questionnaire). Between the ages of 18 and 35. Ability to leg press a weight equivalent to 1.5 times their body mass Exclusion Criteria: • Inability to perform physical exercise (determined by health and activity questionnaire) Taking any other nutritional supplement or performance enhancing drug (determined from health and activity questionnaire). Physical injury preventing the athlete from participating in offseason training Any chronic illness that causes continuous medical care

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Triple


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: Whey + ProHydrolase®<br>Subjects received 250mg of ProHydrolase® along with 25g of whey protein mixed in a drink every visit for 4 weeks | Dietary Supplement: ProHydrolase®<br>* Subjects received 250mg of ProHydrolase® with 25g whey protein mixed together in a drink<br>|
| Active Comparator: Whey<br>Subjects received 25g whey protein in a drink every visit for 4 weeks | Dietary Supplement: Whey protein<br>* Subjects received 25g of whey protein powder mixed to together in a drink<br>|
| Placebo Comparator: Placebo<br>Subjects received 25g maltodextrin in a drink every visit for 4 weeks | Dietary Supplement: Placebo<br>* Placebo<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Amino acid absorption | To examine the amino acid absorption following acute resistance exercise between three supplemental treatments: 1) Whey Protein + ProHydrolase (WPH) 2) Whey Protein (W) and 3) Non-Caloric Placebo (PL) | 4 weeks | 
| mTORC1 pathway | To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on the mTORC1 complex pathway | 4 weeks | 
| Endocrine markers | To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on circulating concentrations of endocrine biomarkers. | 4 weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Amino Acids, Intramuscular Anabolic Signaling, Endocrine Response, Resistance Training