The Effects of L-Arabinose and D-xylose on Intestinal Sucrase Activity in Man

Study Overview
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Brief Summary
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The purpose of this study is to investigate the effect of L-arabinose and D-xylose in a sugar-rich drink on intestinal sucrase activity in healthy volunteers by measuring postprandial blood glucose and insulin, and selected intestinal hormonal responses to increasing doses of L-arabinose and D-xylose.

Detailed Description
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Based on previous scientific results we hypotesize, that pentoses decreases the demand for insulin in metabolizing a given dose of sucrose.

Official Title
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The Effects of L-Arabinose and D-xylose on Intestinal Sucrase Activity in Man

Conditions
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Insulin Resistance

Intervention / Treatment
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* Dietary Supplement: Placebo comparator: 0% pentose
* Dietary Supplement: Active comparator: 4% D-xylose
* Dietary Supplement: Active comparator: 8% D-xylose
* Dietary Supplement: Active comparator: 8% L-arabinose


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy males BMI between 18.4-25 kg/m2 age between 18 and 30 Exclusion Criteria: donation of blood 3 months before or during the study gastrointestinal disorders, diabetes, hypertension, hyperlipidemia, chronic infectious disease (HIV or hepatitis) smoking consumption of more than 21 alcoholic drinks/week elite athletes on medication

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 30 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Placebo Comparator: 0% pentose<br>Pure sucrose without pentoses added. | Dietary Supplement: Placebo comparator: 0% pentose<br>* 14 healthy subjects participated in a randomized double-blinded cross-over study based on four single tests. Sucrose containing drinks were supplemented with two different doses of D-xylose and in addition one dose of L-arabinose was included for comparison with earlier studies. Blood was collected fasting and for 3-h postprandially. Appetite sensations and energy intake were registered. Gastrointestinal symptoms were monitored for 24 hours<br>|
| Active Comparator: 4% D-xylose<br>Sucrose drink supplemented with 4% D-xylose | Dietary Supplement: Active comparator: 4% D-xylose<br> <br> |
| Active Comparator: 8% D-xylose<br>Sucrose drink supplemented with 8% D-xylose | Dietary Supplement: Active comparator: 8% D-xylose<br> <br> |
| Active Comparator: 8% L-arabinose<br>Sucrose drink supplemented with 8% L-arabinose | Dietary Supplement: Active comparator: 8% L-arabinose<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Blood glucose, insulin, C-peptide and GLP-1 | Level of glucose, insulin, C-peptide and glucagon-like peptide-1 (GLP-1) over time, the area under the curve (AUC), the peak value and time to peak | -15, 0, 15, 30, 45, 60, 90, 120, 180 min | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Appetite measurements and energy intake | Appetite measurements: area under/over the curve (AUC/AOC), 3-h mean. Energy intake: the ad libitum energy intake at lunch was registered. VAS was used to asses the palatability (appearance, smell, taste, after-taste and overall palatability) of the ad libitum lunch. | -15, 30, 60, 90, 120, 150 and 180 min | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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L-arabinose, D-xylose, sucrose, glucose, insulin, C-peptide, GLP-1