Effects of Isoflavone Combined With Astaxanthin on Skin Aging

Study Overview
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Brief Summary
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Photoaging is caused by the superposition of chronic ultraviolet (UV)-induced damage on the intrinsic aging process, and accounts for the majority of age-associated changes in skin appearance. Reactive oxygen species (ROS) play a key role in UV-induced skin damage and diminish skin matrix protein levels, leading skin aging. Strategies utilizing endogenous skin antioxidants as well as plant-derived or synthetic compounds have been examined. Astaxanthin mainly from marine algae and crustaceans is a kind of carotenoids which were well-known photo-protective agents with strong antioxidant activity. Several studies have revealed that supplementation of astaxanthin effectively protect skin against UV damage through free radicals. In addition, matrix metalloproteinase-1 induced by UV irradiation is an important step toward skin aging. Recently, many studies pointed out that phytoestrogens exhibit agonistic and antagonistic estrogen activities, suppressing activity of MMP-1 in skin. Isoflavone is a kind of phytoestrogen from soybean and mainly act on skin and bones, inhibiting MMP-1 effectively. The present study is designed to take isoflavone combined with astaxanthin to maximize their anti-aging ability and objectively measure the effects of the mixture on facial wrinkles, hydration, and elasticity.

Official Title
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Effects of Isoflavone Combined With Astaxanthin on Skin Aging

Conditions
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Ageing, Photo-aging

Intervention / Treatment
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* Dietary Supplement: Isoflavone
* Dietary Supplement: Astaxanthin
* Dietary Supplement: Placebo


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Healthy females aged ≥ 45 years Facial wrinkle grade 2 to 4 Exclusion Criteria: received medical or cosmetic treatment that interferes with the general aging process at least 3 months before the study received any hormone replacement therapy at least 3 months before the study reported taking functional foods more than 1 month during last 3 months before the study history of acute or chronic disease such as severe liver or kidney disease history of allergies against any component of trial foods any visible skin disease that might be confused with a skin reaction to the test procedure

Ages Eligible for Study
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Minimum Age: 45 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Treatment
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Isoflavone and Astaxanthin<br>Each subject takes one active tablet per day for 24 weeks. Each tablet contains isoflavone 27mg and astaxanthin 4mg. | Dietary Supplement: Isoflavone<br> <br> Dietary Supplement: Astaxanthin<br> <br> |
| Placebo Comparator: Placebo<br>Each subject takes one placebo tablet per day for 24 weeks. | Dietary Supplement: Placebo<br> <br> |

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change from baseline in skin wrinkles | Facial wrinkles in the crow's feet area measured by Skin Visiometer | Twenty-four weeks | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Change from baseline in skin elasticity | Facial elasticity measured by Cutometer | Twenty-four weeks | 
| Change from baseline in epidermal hydration | Skin hydration measured by Corneometer | Twenty-four weeks | 
| Change from baseline in skin barrier integrity | TEWL measured by Tewameter | Tewnty-four weeks | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Anti-aging, Isoflavones, Astaxanthine, Skin aging