Identifying Early Lung Cancer Cells in Malignant Pleural Effusion Samples From Patients With Primary Lung Cancer

Study Overview
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Brief Summary
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RATIONALE: Studying samples of pleural fluid in the laboratory from patients with lung cancer may help doctors identify early lung cancer cells. It may also help the study of lung cancer in the future. PURPOSE: This laboratory study is looking at malignant pleural effusion samples from patients with primary lung cancer to see if early lung cancer cells can be identified.

Detailed Description
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OBJECTIVES: Isolate and identify lung cancer precursor cells in discarded pleural fluid specimens obtained during routine thoracentesis procedures for the diagnosis and treatment of malignant pleural effusions. OUTLINE: Malignant pleural effusion specimens obtained from the Pathology Department at the University Hospitals of Cleveland are analyzed using an immunomagnetic bead enrichment assay to isolate cells of epithelial origin. The isolated cells are then stained with fluorescent DNA binding dye, Hoechst 33342, and other markers (i.e., CD133, CD24/CD44) and sorted by flow cytometry into progenitor and nonprogenitor subpopulations. The sorted subpopulations are separated and examined functionally by in vitro and in vivo studies. Sphere culture studies are also performed on the sorted subpopulations. The specimens may also be utilized for transcriptional profiling studies, RNAi studies, and for the establishment of long-term cultures and cell lines. DNA is also isolated and stored from tumor and nonepithelial normal cell compartments for future genetic studies on known and unidentified genetic abnormalities contributing to the development of lung cancer, such as EGFR, ErbB2, K-ras, and p53 mutational status.

Official Title
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Isolation and Identification of Lung Cancer Precursor Cells From Malignant Pleural Effusion Specimens

Conditions
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Lung Cancer, Metastatic Cancer

Intervention / Treatment
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* Genetic: microarray analysis
* Genetic: mutation analysis
* Other: flow cytometry
* Other: immunologic technique


Participation Criteria
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Eligibility Criteria
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DISEASE CHARACTERISTICS: Cytologically positive malignant pleural effusion secondary to primary small cell or non-small cell lung cancer Recruited from the Division of Hematology/Oncology, the Multidisciplinary Thoracic Oncology Group of Ireland Cancer Center, or the inpatient or outpatient units of Case Medical Center-University Hospitals PATIENT CHARACTERISTICS: Not specified PRIOR CONCURRENT THERAPY: Not specified
Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Intervention/Treatment |
| --- |
|Genetic: microarray analysis|analyzed using an immunomagnetic bead enrichment assay to isolate cells of epithelial origin|
|Genetic: mutation analysis|analyzed using an immunomagnetic bead enrichment assay to isolate cells of epithelial origin|
|Other: flow cytometry|analyzed using an immunomagnetic bead enrichment assay to isolate cells of epithelial origin|
|Other: immunologic technique|analyzed using an immunomagnetic bead enrichment assay to isolate cells of epithelial origin|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Isolation and identification of lung cancer precursor cells in malignant pleural effusion specimens |  |  | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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non-small cell lung cancer, malignant pleural effusion, small cell lung cancer