Chronic Presence of Epstein Barr Virus in Sulcular and Junctional Epithelial Tissue From Gingiva is Associated With Severe Periodontitis

Study Overview
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Brief Summary
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The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.

Conditions
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Parodontitis Aggressive, Parodontitis Chronic

Intervention / Treatment
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* Procedure: Biopsy
* Procedure: biopsy
* Procedure: Biopsy


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Men exclusively 12 years minimum 2 patients affected by chronic periodontitis 2 patients affected by aggressive periodontitis 2 healthy patients (orthodontics extraction) Subject who have read and understood the information note relative under investigation and who have signed the informed consent Subject whose legal representative signed the parental consent (if minor patient) Exclusion Criteria: Patient with contre-indication to dental extraction Patient witn serious blood disease Patient who exhibits systemic condition incompatible with the realization of the study Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants Patient who presents za buccal carcinoma treated with radiotherapy Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy Patient who presents a linguistic or psychic incapacity to understand information Female patient

Ages Eligible for Study
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Minimum Age: 12 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: chronic periodontitis<br> | Procedure: Biopsy<br>* dental extraction and gingival epithelial biopsy<br>|
| Active Comparator: agressive periodontitis<br> | Procedure: biopsy<br>* dental extraction and gingival epithelial biopsy<br>|
| Active Comparator: healthy patients<br> | Procedure: Biopsy<br>* dental extraction and gingival epithelial biopsy<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Presence/absence of EBV in the epithelial cells of periodontal pockets (by Immunofluorescence and in situ Hybridization |  | at time = 0 (baseline) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Presence/ absence of latency and/or lytic proteins (by immunofluorescence and in situ hybridization |  | at time = 0 (baseline) | 
| - Epithelial tissue infected (by Immunofluorescence and In situ Hybridization) : detection of oral gingival epithelium, sulcular epithelium, junctional epithelium. |  | at time = 0 (baseline) |