Identification of New Predictive Osseointegration Bio-Markers of the Prosthetic Implant in Patients With OsteoArthritis

Study Overview
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Brief Summary
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The study involves the isolation and cellular, molecular and proteomic characterization of synoviocytes, osteoblasts and chondrocytes from tissue samples obtained from patients undergoing surgery of endo or arthroplasty for osteoarthritis (Experimental Group) or interventions for other causes such as for example periarticular fracture requiring the implantation of a prosthesis (Control Group)

Detailed Description
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The study involves the isolation and cellular, molecular and proteomic characterization of synoviocytes, osteoblasts and chondrocytes from tissue samples obtained from patients undergoing surgery of endo or arthroplasty for osteoarthritis (Experimental Group) or interventions for other causes such as for example periarticular fracture requiring the implantation of a prosthesis (Control Group) Deeper analysis of micro Ribo Nucleic Acid (RNA) and Non Coding RNA expressed by the synoviocytes, osteoblasts and chondrocytes will be performed in order to understand their possible involvement in pathways of osteogenic differentiation, through suitable experimental in vitro models of co-cultures. In particular, investigators will focus on the cross-talk between synoviocytes and osteoblasts, cell populations involved in the post-surgical articular microenvironment and on which the osteointegration process of the joint prosthesis depends.

Official Title
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Identification of New Predictive Osseointegration Bio-Markers of the Prosthetic Implant in Patients With OsteoArthritis

Conditions
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Osteoarthritis

Intervention / Treatment
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* Procedure: Surgery for implantation of a prosthesis


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Male and Female patients > 40 years old hospitalized for surgery of endo- or arthroplasty for Osteoarthritis or for surgery for joint traumatic diseases (eg femoral neck fractures) requiring the implantation of a prosthesis Interval of at least three months from any previous infiltrative treatment of any kind; Patients able to provide written informed consent to the study. Exclusion Criteria: Severe cognitive defects or psychiatric disorders; Tumor pathologies or concurrent antineoplastic therapies; Autoimmune diseases (rheumatoid arthritis); Surgery that involves the joint

Ages Eligible for Study
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Minimum Age: 40 Years

Sexes Eligible for Study
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All

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Osteoarthritis<br>Surgery for implantation of a prosthesis in case of Osteoarthritis | Procedure: Surgery for implantation of a prosthesis<br>* Analysis of micro Ribo Nucleic Acid (RNA) expressed by the synoviocytes, osteoblasts and chondrocytes<br>* Other names: non-osteoarthritis arm;|
| Non Osteoarthritis<br>Surgery for implantation of a prosthesis fon non Osteoarthritis cases | Procedure: Surgery for implantation of a prosthesis<br>* Analysis of micro Ribo Nucleic Acid (RNA) expressed by the synoviocytes, osteoblasts and chondrocytes<br>* Other names: non-osteoarthritis arm;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| miRNAs and LncRNA | expression of miRNAs and LncRNA, from synoviocytes, osteoblasts and chondrocytes detected at the time of surgery | at the time of surgery (Day0) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Biomarkers useful for predicting the efficiency of osseointegration of the implant | the mRNAs and the identified proteins will be analyzed through the use of specific software, such as TargetScanPrediction. | at the time of surgery (Day0) | 
| Validation of data obtained on miRNAs and lncRNAs | Through in vitro co-culture systems, investigators will evaluate the role of previously identified miRNAs and lncRNAs in cross-talk between osteoblasts and synoviocytes isolated from patients with osteoarthritis. | at the time of surgery (Day0) |