Invasiveness and Chemoresistance of Cancer Stem Cells in Colon Cancer

Study Overview
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Brief Summary
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The main objective of this study is to identify and characterize subpopulations of cells with invasive capacity in colorectal cancer from primary tumor, blood and metastatic samples.

Detailed Description
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Secondary objectives include: Determine the intrinsic properties essential for the dissemination and chemoresistance of these cells capable of initiating tumors Identify a molecular signature for potential invasiveness and chemoresistance of cells initiating metastases. Describe the evolution of patients during 24 months of follow up and correlations with observed cellular profiles. Enrich the tumor bank of the institution.

Official Title
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Invasiveness and Chemoresistance of Cancer Stem Cells in Colon Cancer: Molecular Characterization and Implications for Therapeutic Strategies

Conditions
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Colorectal Neoplasms

Intervention / Treatment
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* Biological: Samples and follow up


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: The patient must have given his/her informed and signed consent The patient must be insured or beneficiary of a health insurance plan Patient with adenocarcinoma-type colorectal cancer: stage III at diagnosis, surgical resection of the primary tumor proposed. stage IV at diagnosis, surgical resection of the primary tumor and possibly of metastases proposed. Stage IV who have already undergone surgical excision of the primary tumor, and for whom metastasectomy is now proposed. Exclusion Criteria: The patient is participating in another study The patient is in an exclusion period determined by a previous study The patient is under judicial protection, under tutorship or curatorship The patient refuses to sign the consent It is impossible to correctly inform the patient Patients for whom surgical resection of the primary tumor is not considered as an option PStage IV at diagnosis, but metastasectomy is not considered as an option Patients with positive HIV, Hepatitis B or Hepatitis C serology

Ages Eligible for Study
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Minimum Age: 18 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| 30 Patients<br>Patients with operable, stage III or IV, adenocarcino-type colorectal cancer treated at the Nîmes University Hospital. Intervention: Samples and follow up | Biological: Samples and follow up<br>* Samples: Peroperative blood sample plus primary and metastatic tumor biopsies. Follow-up: disease outcomes assessed at 24 months<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Ability to maintain the cells isolated from colorectal tumors in culture or 3D collagen matrices and then infect these cells to make them express reporter genes: yes/no. |  | Baseline (Day 0) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Serology HIV |  | baseline; day 0 | 
| Serology Hepatitis B |  | baseline; day 0 | 
| Serology Hepatitis C |  | baseline; day 0 | 
| Location of primary tumor | right colon, left colon, transverse colon, sigmoid, rectum | base line; day 0 | 
| Age at diagnosis |  | baseline, day 0 | 
| Metastases from the outset: Yes / No |  | baseline, day 0 | 
| Resection proposed: yes/no |  | baseline, day 0 | 
| Chemotherapy proposed? yes/no |  | baseline, day 0 | 
| Number of metastases |  | 24 months | 
| Resection performed: yes/no |  | 24 months | 
| Number of chemotherapy sessions performed |  | 24 months | 
| Objective tumoral response to treatment? yes/no |  | 24 months | 
| Tumor recurrence: yes/no |  | 24 months | 
| Vital status | living/deceased | 24 months | 
| Ability to establish tumor xenografts from injected cells: yes/no. |  | baseline; Day 0 | 
| Ability to detect subpopulations of tumor cells expressing fluorophores by flow cytometry after isolation: yes/no |  | baseline; day 0 | 
| Characterization of mRNA expression profiling and micro-RNA + in vitro EMT cells |  | baseline; day 0 | 
| World Health Organisation Score |  | 24 months | 
| Tumor staging |  | 24 months | 
| Number of surgeries performed |  | 24 months | 
| Number of circulating cancer cells per ml blood |  | baseline; day 0 | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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invasive potential, molecular characterisation of circulating cells