Physiological Effect of Ataulfo Mango (Manguifera Indica) Beverage in Exercise

Study Overview
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Brief Summary
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The aim of this study is to assess the antioxidant capacity of two mango base beverages (AM1, AM2), against exercise-induced oxidative stress.

Detailed Description
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Introduction. The post-consumption physiological effect of mango cv. Ataulfo pulp (MP) and peel (MC) in humans before exercise has been poorly evaluated. Objective. To evaluate the physiological response, to consumption of two mango base beverages, AM1 (200g MP/ 600mL water) and AM2 (160g MP; 40g MC / 600mL water), before and after a submaximal exercise test. Methods. The antioxidant profile and physicochemical characteristics of AM1 and AM2 were evaluated. Several cardiorespiratory, anthropometric and body composition parameters of 19 university students, prior to the consumption of AM1, AM2 and water, were evaluated in three non-consecutive sessions (self-control cases). The glycemic, lactic and antioxidant (FRAP), uric acid (AU), reduced glutathione (GSH) and lipid oxidation (TBARS) and protein carbonyls (PC) responses were evaluated in plasma before and after an incremental cycle ergometer exercise test.

Official Title
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Physiological Response to Ataulfo Mango Pulp and Peel Beverages in Exercise

Conditions
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Exercise-induced Oxidative Stress

Intervention / Treatment
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* Dietary Supplement: Mango pulp beverage
* Dietary Supplement: Mango pulp and peel beverage
* Dietary Supplement: Control beverage


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: healthy male university student no alcohol, cigar or drugs consumption Exclusion Criteria: no antioxidant supplement or ergogenic consumption health issues with performing physical activity

Ages Eligible for Study
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Minimum Age: 17 Years
Maximum Age: 28 Years

Sexes Eligible for Study
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Male

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Prevention
Allocation: Randomized
Intervention Model: Crossover Assignment
Interventional Model Description: The prescription of the treatments (AM1, AM2) and control (water) was randomized. The subject consumed 600mL of AM1, AM2 or water. 2 hours later subject performed the exercise test. Next treatment was evaluated after a week of wash-out.
Masking: None (Open Label)


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Experimental: AM1<br>Mango pulp beverage | Dietary Supplement: Mango pulp beverage<br>* The subject consumes mango pulp beverage (200 g mango pulp + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.<br>* Other names: 200 g mango pulp + 600 mL water;|
| Experimental: AM2<br>Mango pulp and peel beverage | Dietary Supplement: Mango pulp and peel beverage<br>* The subject consumes mango pulp and peel beverage (160 g mango pulp + 40 g mango peel + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.<br>* Other names: 160 g mango pulp + 40 g mango peel + 600 mL water;|
| Placebo Comparator: Control<br>Control beverage | Dietary Supplement: Control beverage<br>* The subject consumes control beverage (600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.<br>* Other names: 600 mL water;|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Antioxidant capacity change | Assessed by FRAP change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis. | 0 min (before beverage intake), 9-15 min (immediately after exercise test) | 
| Antioxidant capacity change | Assessed by uric acid concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis. | 0 min (before beverage intake), 9-15 (immediately after exercise test) | 
| Antioxidant capacity change | Assessed by reduced glutathione concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis. | 0 min (before beverage intake), 9-15 (immediately after exercise test) | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Oxidative stress change | Assessed by TBARS concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis. | 0 min (before beverage intake), 9-15 (immediately after exercise test) | 
| Oxidative stress change | Assessed by protein carbonyls concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis. | 0 min (before beverage intake), 9-15 (immediately after exercise test) | 
| Lactic acid concentration change | Assessed by lactic acid concentration change. Capillary blood samples were taken from the fingertip, using a lancet and analyzed (YSI modelo 1500, Yellow Springs, USA) | 0 min (before performing exercise test), 3 min, 6 min, 9 min, 12 min, 15 min (during exercise test) | 
| Postprandial glycemic change | Assessed by blood glucose concentration change. Capillary blood samples were taken from the fingertip, using a lancet, and analyzed (ReliOn Confirm/micro Test Strips ARKRAY, USA) | 0 min (before beverage intake), 30 min, 60 min, 90 min, 120 min (after beverage intake) | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Exercise, Oxidative stress, Antioxidant