Effect of a Single Dose of Cranberry Beverage on Inflammation and Oxidative Stress

Study Overview
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Brief Summary
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The purpose of this study is to determine the effects of a single dose of cranberry beverage in healthy adults on the kinetic uptake of polyphenol compounds and polyphenol metabolites, and antioxidative and anti-inflammatory activity.

Detailed Description
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Participants will be randomized based on age, sex and BMI, and will consume a low-polyphenol diet 2 days prior to the intervention. On the day of the study, baseline blood and urine samples will be collected after an overnight fast. Participants will consume the assigned beverage within 15 minutes and blood collected at 2, 4, 8, and 24 h (10 mL/blood draw; total blood volume per period = 50 mL). Urine will be collected for polyphenol analysis during the following periods: 0-3, 3-6, 6-9, 9-12, 12-24 hours. After a one-week washout period, participants will receive their second and then the third beverage in a cross-over design; blood and urine sampling will be repeated at each of the 3 intervention periods.

Official Title
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Acute Bioavailability of Cranberry Polyphenols After A Single Dose In Healthy Adults and Its Antioxidative and Anti-Inflammatory Action

Conditions
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Inflammation

Intervention / Treatment
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* Other: Low Calorie Cranberry Juice Cocktail
* Other: Cranberry Extract Beverage
* Other: Non-Cranberry Beverage


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: Men & women; aged 18-35 years BMI: 19-25 kg/m2 Exclusion Criteria: Any significant chronic disease, hypertension, ulcers, dyspepsia, lactose intolerance, allergy, psychotic illness Had major gastrointestinal surgery or on prescription or surgical treatment Any condition that may interfere with the digestion, absorption, metabolism or excretion of nutrients Regularly smoked in the previous 6 months On medication or ingested a prescribed drug at any time within the 14 days preceding study enrolment (excluding hormonal contraceptives and hormone replacement therapy) or over-the-counter preparation within 7 days preceding enrolment Risk factors for AIDS or known HIV positive status Pregnant or lactating High intake of chocolate or similar high polyphenol foods Regular intake of vitamin and polyphenol supplements Drug and alcohol misuse Currently participating or had participated in another clinical study during the previous 3 weeks Donated blood in the previous 3 weeks Exercise more than 3 times/week for longer than 45 min each time

Ages Eligible for Study
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Minimum Age: 18 Years
Maximum Age: 35 Years

Sexes Eligible for Study
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All

Accepts Healthy Volunteers
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Accepts Healthy Volunteers

Study Plan
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How is the study designed?
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Design Details

Primary Purpose: Basic Science
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double


Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| Active Comparator: Low Calorie Cranberry Juice Cocktail<br>Beverage containing cranberry: one dose of 15.2 ounces consumed within 15 minutes | Other: Low Calorie Cranberry Juice Cocktail<br>* Beverage containing cranberry at a dose of 15.2 ounces consumed within 15 minutes<br>|
| Active Comparator: Cranberry Extract Beverage<br>Beverage containing cranberry: one dose of 15.2 ounces consumed within 15 minutes | Other: Cranberry Extract Beverage<br>* Beverage containing cranberry at a dose of 15.2 ounces consumed within 15 minutes<br>|
| Placebo Comparator: Non-Cranberry Beverage<br>Beverage absent cranberry: one dose of 15.2 ounces consumed within 15 minutes | Other: Non-Cranberry Beverage<br>* Beverage absent cranberry at a dose of 15.2 ounces consumed within 15 minutes<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Reduced Glutathione (GSH) Concentrations in Red Blood Cells |  | 0, 2, 4, 8, 24 h | 
| Superoxide Dismutase (SOD) Activity in Red Blood Cells |  | 0, 2, 4, 8, 24 h | 
| Glutathione Peroxidase (GPx) Activity in Red Blood Cells |  | 0, 2, 4, 8, 24 h | 
| Oxidative Damage to DNA Assessed by Plasma 8-hydroxy-2'-Deoxyguanosine (8-OHdG) |  | 0, 2, 4, 8, 24 h | 
| C-Reactive Protein (CRP) Concentrations in Plamsa |  | 0, 2, 4, 8, 24 h | 
| Nitric Oxide (NO) Concentrations in Plamsa |  | 0, 2, 4, 8, 24 h | 
| Interleukin-1alpha (IL-1a) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Interleukin-1beta (IL-1b) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Interleukin-2 (IL-2) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Interleukin-4 (IL-4) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Interleukin-6 (IL-6) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Interleukin-8 (IL-8) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Interleukin-10 (IL-10) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Interferon-gamma (IFN-y) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Tumor Necrosis Factor-alpha (TNF-a) Concentrations in Plasma |  | 0, 2, 4, 8, 24 h | 
| Urinary Anti-bacteria Adhesion Activity |  | 0, 3, 6, 9, 12, 24 h | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Inflammation, Oxidative Stress, Vaccinium macrocarpon