The Effect of Vitamin D on in Vitro Fertilization Outcome

Study Overview
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Brief Summary
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To date, several studies have been done to explore the relationship between Vitamin D level and IVF outcome; however, the results are controversial. Sample sizes in these studies mentioned above were relatively small. In addition, there are no prospective cohort studies in this field. Thus, here the investigators carry this prospective observational cohort study to demonstrate the relationship between vitamin D level and IVF outcome.

Detailed Description
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Vitamin D level is measured before the commence of IVF cycle. According to Vitamin D level, patients are divided into 3 groups: A, Deficient, <20 ng/mL; B, Insufficient, 20-29.9 ng/mL; C, Replete, >30 ng/mL. Ongoing pregnancy rate are compared among these three groups after controlling age, number of embryos transferred, ect. In addition, Vitamin D level is also meassured before embryo transfer in FET cycles with PGT treatment. The effect of Vitamin D on IVF outcome is detected in patients transferred with Euploid embryos.

Official Title
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The Effect of Vitamin D on in Vitro Fertilization Outcome, a Prospective Observational Study

Conditions
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Ongoing Pregnancy Rate, Vitamin D, in Vitro Fertilization

Intervention / Treatment
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* Procedure: Embryo transfer


Participation Criteria
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Eligibility Criteria
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Inclusion Criteria: age between 21-40 years old basal FSH hormone level less than 10 mIU/mL the first cycle of IVF treatment with standard long protocol both ovarian present and normal uterus. Exclusion Criteria: Important data missing Gametes donation cycles Anyone that does not meet the inclusion criteria

Ages Eligible for Study
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Minimum Age: 21 Years
Maximum Age: 40 Years

Sexes Eligible for Study
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Female

Accepts Healthy Volunteers
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No

Study Plan
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How is the study designed?
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Arms and Interventions

| Participant Group/Arm | Intervention/Treatment |
| --- | --- |
| A1, Vitamin D deficient<br>Vitamin D level before cycle < 20 ng/mL. | Procedure: Embryo transfer<br>* For patients in each group after oocyte retrieval, they will have embryo transferred 3 or 5 days after oocyte retrieval. For FET cycles, they will have ET with an euploid blastocyst.<br>|
| B1, Vitamin D insufficient<br>Vitamin D level before cycle 20-29.9 ng/mL | Procedure: Embryo transfer<br>* For patients in each group after oocyte retrieval, they will have embryo transferred 3 or 5 days after oocyte retrieval. For FET cycles, they will have ET with an euploid blastocyst.<br>|
| C1, Vitamin D replete<br>Vitamin D level before cycle > 30 ng/mL | Procedure: Embryo transfer<br>* For patients in each group after oocyte retrieval, they will have embryo transferred 3 or 5 days after oocyte retrieval. For FET cycles, they will have ET with an euploid blastocyst.<br>|
| A2, Vitamin D deficient<br>Vitamin D level before ET < 20 ng/mL (FET with Euploid embryos) | Procedure: Embryo transfer<br>* For patients in each group after oocyte retrieval, they will have embryo transferred 3 or 5 days after oocyte retrieval. For FET cycles, they will have ET with an euploid blastocyst.<br>|
| B2, Vitamin D insufficient<br>Vitamin D level before ET 20-29.9 ng/mL (FET with Euploid embryos) | Procedure: Embryo transfer<br>* For patients in each group after oocyte retrieval, they will have embryo transferred 3 or 5 days after oocyte retrieval. For FET cycles, they will have ET with an euploid blastocyst.<br>|
| C2, Vitamin D replete<br>Vitamin D level before ET > 30 ng/mL (FET with Euploid embryos) | Procedure: Embryo transfer<br>* For patients in each group after oocyte retrieval, they will have embryo transferred 3 or 5 days after oocyte retrieval. For FET cycles, they will have ET with an euploid blastocyst.<br>|

What is the study measuring?
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Primary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Ongoing pregnancy rate | 12 weeks after embryo transfer, gestational sac with fetal heart present inside uterus | 12 weeks after embryo transfer | 

Secondary Outcome Measures

| Outcome Measure | Measure Description | Time Frame |
| --- | --- | --- |
| Ectopic pregnancy rate | Ectopic pregnancy is defined as serum HCG positive, yet with no gestational sac present inside uterine cavity | 35 days after embryo transfer | 

 Terms related to the study
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Keywords Provided by Centre Hospitalier Valida
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Vitamin D, IVF outcome