The following text describes a protein:
Function:
Guanine exchange factor that acts as an activator of ARF1 at the trans-Golgi network and is thus involved in vesicular budding and traffic between compartments of the Golgi apparatus. Activation of Arf (ADP-ribosylation factor) GTPases is essential for vesicle formation via recruitment of cargo adapters and coat proteins necessary for Golgi trafficking. Also plays an essential role in ER-to-Golgi traffic. SEC7 also acts as an effector of two Rab GTPases, YPT1 and YPT31/32.
Miscellaneous:
MISCELLANEOUS: Present with 3670 molecules/cell in log phase SD medium.
Subcellular Localization:
Cytoplasm. Golgi apparatus. Golgi apparatus, trans-Golgi network. Cytoplasmic vesicle, COPI-coated vesicle membrane. Cytoplasmic vesicle, COPII-coated vesicle membrane. Note=Recruited to the trans-Golgi network by positive feedback via its HDS1 domain by interaction with activated ARF1.
Domain:
The highly charged acidic domain may serve a structural role to interact with lipids or proteins on the cytoplasmic surface of the Golgi apparatus; The homology upstream of Sec7 (HUS) box is necessary for the allosteric activation of SEC7 by YPT31; The homology downstream of Sec7 (HDS) domain 1 is required for recruitment to the trans-Golgi network via its interaction with activated ARF1; A 50-amino acid region near the C-terminus called the as the C2 domain-interacting region (CIR) is required for RSP5-binding and for normal SEC7 localization.
Sequence:
MSEQNSVVNAEKGDGEISSNVETASSVNPSVKPQNAIKEEAKETNGEDQKCKGPENAGSTAETKETSNDATNGMKTPEETEDTNDKRHDDEGEDGDEDEDEDEDEDEDNGDEDDEDVDSSSSETSSEDGEDSESVSGESTESSSGEDEESDESDGNTSNSSSGDESGSEEEEEEEEEEEEEENAGEPAIAHQDSVPTNDSTAPRSTHTRNISLSSNGSNTNSTIILVKTTLETILNDKDIKKNSNAQKAIERTLQKFKEFDPQTTNNPHYVDSILVFEALRASCRTKSSKVQSLALDCLSKLFSFRSLDETLLVNPPDSLASNDQRQDAADGITPPPKQKIIDAAIDTISDCFQGEGTDDRVELQIVRALSSCILEEDSSSLCHGASLLKAIRTIYNVFVFSLNPSNQGIAQATLTQIISSVYDKIDLKQSTSSAVSLSTKNHQQQSAIELSEASENAETPAPLTLENMDKLNDDEERLMDAQQPDSIAITNQDLAVKDAFLVFRVMAKICAKPLETELDMRSHAVRSKLLSLHIIYSIIKDHIDVFLSHNIFLPGKERVCFIDSIRQYLRLVLSRNAASPLAPVFEVTLEIMWLLIANLRADFVKEIPVFLTEIYFPISELTTSTSQQKRYFLSVIQRICNDPRTLVEFYLNYDCNPGMPNVMEITVDYLTRLALTRVEITQTQRSYYDEQISKSLSTYNFSQLPLLTSSNLSSSPDVGQVNLLFPLDFALKMVSLNCIVSVLRSLSSWAHKALNPNTHTANKVLLNTTSSARQESRSSLSNDVRSSIMTSNDDFKPTYEDEESRSLSSQNIDADDPTQFENLKLRKTALSECIAIFNNKPKKAIPVLIKKGFLKDDSPISIAKWLLETEGLDMAAVGDYLGEGDDKNIAIMHAFVDEFDFTGMSIVDALRSFLQSFRLPGEGQKIDRFMLKFAERFVDQNPGVFSKADTAYVLSYSLIMLNTDLHSSQIKNKMSLQEFLENNEGIDNGRDLPRDFLEGLFNEIANNEIKLISEQHQAMLSGDTNLVQQQQSAFNFFNSRDLTREAYNQVSKEISSKTELVFKNLNKNKGGPDVYYAASHVEHVKSIFETLWMSFLAALTPPFKDYDDIDTTNKCLEGLKISIKIASTFRINDARTSFVGALVQFCNLQNLEEIKVKNVNAMVILLEVALSEGNYLEGSWKDILLVVSQMERLQLISKGIDRDTVPDVAQARVANPRVSYESSRSNNTSFFDVWGKKATPTELAQEKHHNQTLSPEISKFISSSELVVLMDNIFTKSSELSGNAIVDFIKALTAVSLEEIESSENASTPRMFSLQKMVDVCYYNMDRIKLEWTPLWAVMGKAFNKIATNSNLAVVFFAIDSLRQLSMRFLDIEELSGFEFQHDFLKPFEYTVQNSGNTEVQEMIIECFRNFILTKSESIKSGWKPILESLQYTARSSTESIVLKTQLLVSNDIVTNHFENVFSQEDAFSELVGVFREITKNKRFQKLSLHALESLRKMTQNVADICFYNENKTEEERKHNDALLRGKDIFQDVWFPMLFCFNDTIMTAEDLEVRSRALNYMFDALVAYGGKFNDDFWEKICKKLLFPIFGVLSKHWEVNQFNSHDDLSVWLSTTLIQALRNLIALFTHYFESLNRMLDGFLGLLVSCICQENDTIARIGRSCLQQLILQNVSKFNEYHWNQIGDVFDKLFDLTTANELFDYDPLQQGRKSSVSHHQTTNDTSQHSDDDSNDRRENDSNISETVERAHQEESSEDVGGDMVETLNGQTKLNNGNSVPTVKDELNPKPASLSIPKKTKHMKRNESNEDIRRRINIKNSIVVKCVLQLLMIELLNELFENEDFAHCIPYKEAIRITRLLEKSYEFSRDFNEDYGLRTRLVEARVVDKIPNLLKQETSAAAVLLDIMFQLYLNDDEKKADLITRLITICIQVVEGYVSLDDRTMERSINAWRSVIVEILQGYYEFDDEDFRLYCPAMYALVIQILDKSVPTELRHAIKQFLSRVGELYLSTD