The following text describes a protein:
Function:
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of malformins, cyclic pentapeptides with a disulfide bond between 2 consecutive cysteins, that show potential anti-tumor as well as antimalarial and antitrypanosomal properties. The nonribosomal peptide synthetase mlfA is responsible of the formation of the cyclic pentapeptide (Probable). The malformin biosynthesis clusters in malformin-producing fungi also contain enzymes involved in the formation of the disulfide bond between the two consecutive cysteins within malformins, in addition to additional tailoring enzymes such as methyltransferases or oxidoreductases. They are also composed of up to 4 major facilitator superfamily transporters, and transcription factors probably involved in the regulation of the expression of those clusters (Probable).
Domain:
NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for L- to D- amino acid conversion) are present within the NRP synthetase. MlfA has the following architecture: A-T-C-A-T-C-A-T-C-C-A-T-C, with the functions of the five condensation domains during malformin biosynthesis being DL-joining (epimerizing subtype), LL-joining, epimerization, DL-joining and cyclizing domain, respectively.
Sequence:
MSRFSCIFPTLTDGYVPNLDQTRAAGRRTYTIDRRGWTAPSSQTESHILAAWGLVLSSYVGTDEVAFYIVPTTGPDTTALAELKVEGDMSRRSLINAAEQLLHPGPVGAGQVSGESANTIITFEKDIESLFVTQAEAANVGTAMAQALAEVGADDHNRLIKNLNLMSPTHLESIWQFNANVPGMWEECFHDVIERRAANRPHSLAVDAWDMKLTYADLVREARLLAAYLQHRGVRPGSVVPISFERSGAALVAMLAVSKAGGAFVSVPPTLPAGRLDAILEVIEAPFVVTWSKYEPFWAERLPTLPIDSYPKPSADATVKTLGKPEDLFYVIFTSGSTGRPKGCMLSHSNWLNGALRNAPSWKYGPESRVLQMLSHTFDMSLLEICTSLGSGACVCVPRTEEIETSVSDAINRWQVNHVIMTPSLARSLRRDDVPGLKTMCLGGEAFPREIVTMWSERINLWQFYGPSECSINSSSRPITRPDADPLNIGPPNSAACWVVDTQDYNKLVPVGAIGELLVSGPIVGMGYLKNPIKTAEAFLDEVGFVAKDDPQFGGFRFYRTGDLVRWNSDGTITFCGRADTQVKLNGQRLELAEVEYQLGLEAGVQYAIAMAPQSGRCKNNLIAVLTVKCGGASNQGNADDEIPLLDRHDPIVQQTVKKLRSQLQHALPRYMVPTIWAFVGRMPMSPSGKIDRVQLRNWVQEMSQETFDAITGRSFEAEDHVLGLSQLEQEIQLAWAEALGLSAAEVGLQQPFVALGGDSIKALDAVARCRARQIKISMVHILSCEGVREASSLAEVQETPAHQVAEIAVDYSDLWTRLSTEYDISKLGVTQVEEVEDVFPCTTMQEGMFLGQIRRPGAYHMRFFHRVQLKGGSLPTVERIQQAWASLVERHPSLRTVFVDDLSSEAIYHSVVLRSVPMELTMREVPRDLNPESALAMFTEELVPFRPNAPLHRMLLLTCRGRVPYLMLEISHVIMDGYALSVFRREFIRACSSTASLPRGPDYRMFANYHRTRQTDESAKYWTNYLADCVPCHIPTDPLSVPTDASPEWPRTLQRRDFGFDNSVAFLQRCKERQVTLACAIRAAWALVLRAYTQSRDVCFGYVSSGRNVPVPEVETIFGLCLSMQVCRAKLSEASTIASLARKIQEDYVASLPFQHYPLAEAQRGLKQTHGQGLFNTAISMEWVPPTAEDEDALLDLEEIREQDDPTEYDIAISVDIHEGHIKLGFLYWPNLSDFQITHLAEALQGAMNCFAFQPDVALNTLTLLQASDLCSTLTNGPTLLPLEAVRGNVISMIDRWVTRQPESPAIDGWDGSLTYKQLHEQSSWVARNLLHQGVKLGDRILVCADRSSRTVVTILGVVRAGCVLVLSNPTDPEKRLQWLAHKCNATLVVADPAYEERFATSGARVFSTTSVCAPAAWDYEFSALDDQDLVSILFTSGSTGTPKGILMDHGALATSVLLGHGRTLRFSRHTRMLHFASLTFDAALAEIFTTLAHGGCICVPCEEDRLSDVSGCISRFAVNTAMLTPSVGRLLDPEALPTLKALAMIGEPMSRLDVERFAPVLDLYNGAGPTETSIMVTIAGPMKPTDEPVNLGYAVAGVRLWVTEAENPNRLAPLGAVGELIVEGRLVTRGYLDDPARTRESFLPNLPWLPSQHALYRTGDLVRYAEDGSLRYMGRKDTQVKLRGQRIELQEVEYHLRKSLQQAQVVVEMVIPEGKIRAQASLVAFVSGLTAADVESSSARNFDQSMPMSQIALPGSTIQALEEALPRYMIPSVYFALDTIPLSVNGKADRRRLREIGAALLVSSTAHKNTVDGKSEPVKWTASSKLELTLLELWTTTLGLEAETIYGDDSFFELGGDSVSAMKLVATARDRFKLSLSVPQMFRHPTIHQLAAILGEATGQPESSASSTTEEGFTFSTPDDSSTNDGVDDDFLRLATAQLAQLAQEKGKKVDIAALLKQLQGGSSSCKTPSVSSSSSSSSSRKKKSAKVVSPVEAPAPVPVPFSLLDGGADVVEKIRAQAVEQCKILPGDIEDIYPATALQEGMMALMARTPGVYTTTLTCELPERVNLARLHSAWDKAAEAHPILRTRIILTENNTAVQVVQRAKELPWDAYSLQDGDPLPDLTSNMTLGSTLLRLAEIHRQNQPRMLLVAIHHALYDGWSMPLLKQAVEDVYHGQELWPQPFTPFINYLNEGKPAAQGYWTAHLDGFAGSVFPNLPSINHHIQPTERRTRSLAVPTAPPGSQYTMATKIQAAWAVTVSRYAEAEDIVFGTVSTGRSAPVPSIDRMVGPTITTVPVRISLGNQAERLTSLLQRVQEDGWNRMDHEHLGLQHIRRLGESAAAACNLQTLLVIQPREEPRAKSISTLLSGLQDVAELKGVDTYPLMLVCEPDGVSLHLTAVFDPAVLDAVMLDRMLAHWELVLNQIWSEPDMAVMGLDGVSYRDKQTLVRWNAGEKIADGCAHDAVYEWSVRTPHAPAVFAWDGKWTYEELEKCSSLIASQVLVHGVSSGDFVALYHEKSRWAAAAILAVFKAGGILVTLDPAHPKDRIKDILDQARPRLVLTSQSLLDEARELETPVMVVQFAASQPMPGECFPLPTVSPTQAAYAPFTSGSTGRPKGIPLEHRGLAASTASVARACLLRPASRVLHFASFAFDASMMEHLIAWHAGSCLCIPVETVRQTDLARCIRDFEVTWAFLTPSCLRLISPDDVQSLEALGLGGESMTPEDIFIWGPRLRQIVQLYGPAECSIVAALTEVTKPSENRLIGRPNACRCWVVDPHSPDRLAPLGAVGELVIEGITVGRGYIDDPERTTQAFIPPPTWIQTLYPNEQQPSRLYRTGDLVRYAGTDGKLTFIGRRDGQLKLHGQRIELADVEAHLRPLIPGTQKVVVEMVHSVGNHHPLLAAFVEEILTSQDQVEQVVNLLHPSQTQCALNVKAIDSALSQTVPQYMIPSMYLHISRLPLSASGKLNRRHLRRLVAEFPRQRLSEYAAGSGLAVPNRPATAQEREMQAIWARVLSVDPDTIGVNEDFFRIGGDSISGMQVATRCNAAGMHITSADLFQHRTIEQLMRHLSANGKTGSASISLPPEPVDEWVPLAPIQQLFFEIAPQGPDHFNQSLLLRTSRRVSAEKLAGGLDILVGRHSMLRARFCRDDSGQWSQQVRSRGPYPASAFYRLTTHNHIAPELLSSLLAASQMALSIQEGPLLAVDLVNLTDDTQLVYLVAHHLIIDLVSWRILHAELEEYLQTGSFASTTGSVPFLTWSRAQAEYSANHLTPTLPLPGFQEANDGFDASRYWGISCESNTFGQTSTSTFTLDQTVTDQLFGPANNVLDTRPAEILQAALWYSFTQSLTDRPGPSIYVEGHGREPWTGSIDLSGTVGWFTTMSPLVSAPWDSLSQTSMRDFLDALSYIKDQRRRIPANGWAYFTSRYLNDEGKVAYGRMKPVVEILFNYMGQYQEMNREDAILQLAGDGIQSGTGAADVADNVPRFSLIDVSAFISNGCLTFQFILPKSLQQDSRLQGCFQEYERTLVAAANSLSTEGPRKTLADFPLMPALTYDQLSQCLDHTLPSMGLCARDVVDIYPCSPVQQGMLLAQLRDRQAYQQRFRFQVKSRGSTDRLTLEKLKDAWTEVINRHDILRTLLLPVSDYSHLDQVVMAPGSLQHLVRINAMDTNPTQGLPHSINITSDSTGTVICEWNVSHALVDAMSIAVIQQEVNEALEGSLGQHQKTPRYADYIQWLSLQDNTETQAYWKKYLEGVEPCLFPKLASSTDKVNPEGTISAIRATWTRDSRLDKLCHTHGITLTNLFHIVWSLLLSAYLGTDKVCFGYTTLGRDVPVDGVEKMVGPLVNVIATTIQLQEDDSILDALLTHQTHLSNSLQHQHYALADVYASLGLVGSQLFNTIVSLQDISHFDANDERPTRVEMLPANDVSEYDVALNIGVDQSSIQVVCSYRTLSLSVEQADALLRTTSHVLDEILRDPKQPLRDLEVISPQCKEQLVKWNAAMPAPTDEYIHEKIQDQCRLHSSREAACAWDGIFTFAEVDDLSSRLAARLIRMGVTSGHIIPIYSPKSRWTVIAILGVLKTGAAFTLLETSHPTARLRVICNEIKADIIIAPASHAVPAATLAPILVVLDSITSMSPQESDLLPAVGMPPAAEALAYLIFTSGSTGNPKGVMVTHQNLCSNASIMTTSVNMTSDSRVLHFASHAFDACLWEIFGALFAGACLIIPSESETKEDLAGCIERMVVTWAFLTPSVARILKPEALPSLRNLVLGGEPIAASDLDMWRGHVQVVCAYGPTETAILASTTSPSTFPSDGKDIGVPTGSSLWIVDKQNYNKLAPHGATGELLIEGPNVSQGYLGDPEKTNEAFPVAPRWLSQLRKSPTRVYRTGDLVRFNTSTGTIHFVGRKDNQIKFHGQRIELGDIEHHAQQAFSNASMVIVDLITPEQPQQPYIVAFVHQADTRTGTADPIDTILLPPSESFRADAIGAQNHMHKRLPHYMVPTAFLPLHRLPLSGTGKADRKRLRQCALSLSSLELNAYRATASAKRMPFTAAECKMQELVATVLGRDMSEIGMDDSFFYLGGDSIQAMRLVSEGRQQGLTLSLQAIFDAPRLGDLAYRTANLVRVSEPAPPTLPATSSDDCDHKETIVAALPIKKTDVAEVLPTTSFQRTWLDSQLKSYIVVDIPGPIDLARLRTAIQRVVKAHPILRASFVPYETTTMQVILRTAVVITKADLSTTTVEGICRKDANAPMAPGTPYLRVILATQGEVDRKLIMRLSHAQYDGISLSLLMNDLSHAYASESRPLPSSHLPAFNDYITYQQTQGADPTATTFWHRLLKDVPITYLDLQPAETPTSNGSLITRTRDINIAAFPSLPNGITTATAVKAAWSLVLAQKTGSLAVIFGQVVHGRGIALTGVEGIVGPCANITPVVARLGPQTTRMELMQTLQDQHRSAMPYETAGRKELQTIVQHQNNVMADDMELSLGEARCRVDLRAVDHVPQEVWVYSSIDGGRPGMLEVKIMSSTLVLSEEVAEELMDLLVEMMKRLFSDPEGVCV