The following text describes a protein:
Function:
Partially reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of fumigermin that inhibits germination of spores of the inducing S.rapamycinicus, and thus helps the fungus to defend resources in the shared habitat against a bacterial competitor. The partially reducing polyketide synthase fngA alone is sufficient for the production of fumigermin. FgnA catalyzes the condensation of 3 malonyl-CoA units to an acetyl-CoA starter, and 3 methylations to yield fumigermin. It is remarkable that the five cluster genes including fgnA are conserved in distantly related fungi, supporting the assumption of a fumigermin cluster; it is thus possible that originally all five genes were functional, but that the genes encoding tailoring enzymes became inactive from mutations, similar to the case of the fgnA gene in strains A1163 and Af293 (Probable).
Domain:
Multidomain protein; including a ketosynthase (KS) that catalyzes repeated decarboxylative condensation to elongate the polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects and transfers the extender unit malonyl-CoA; a dehydratase (DH) domain that reduces hydroxyl groups to enoyl groups; a methyltransferase (CMeT) domain responsible for the incorporation of methyl groups; a ketoreductase (KR) domain that catalyzes beta-ketoreduction steps; and an acyl-carrier protein (ACP) that serves as the tether of the growing and completed polyketide via its phosphopantetheinyl arm.
Sequence:
MSFPQVDVPSLPVGSASTQDTSPVSGPFNSSDSDHDIVNGGQANGSPFTEPIAICGLGLRLPGGIRDGDSFWDLLVNGRDARMPIPASRYNISGFDGSLDGRDPIKTTHGYFLDEDLSSLDASFFSMTKTELEKCDPQQRQLLEVTRECLEDAGETDYRGRNVGCYIGNFGHDWMEISLREPQHSRSYNVLGYSDMILANRVSYEYDLRGPSVVIKTACSASLVALHEACRALQARDIPSAIVGGTSLILAPTLTSNFFGEGILSPEASCKTFDESADGFARAEGVTAIYVKRLDDALRDGNPIRAVIRGTGTNSDGKSMGIMSPSAEAHEALMRQVYDQAGLSPRETAFVEEKRRLSNASKCHGTGTATGDPIEATAVGNVFSERGVYIGSVKPNVGHSEGCSGITSLIKAVLALEHKTIPPNIKFRNPNPKIPFVEKKLVVPIQPTPFPLDKAERISVNSFSIGGSNAHIDAYKSYARDHPDAISDIAYTLAVRRERLPHRAFAIWQNGELQTSSLSKASAVPPAITMIFSGQGAQWAEMGKKLIETEASFRHDLASMDSILQSLRKPPCWSILEELQKPAEDSQINRAEMAQPLCTALQVALFHQLKRLGIKPTAVVGHSSGEIAAAYAAGYITLEYALAVAYYRGYITKNADRSRGAMAAVRLSATEVLPFLRTGVCIACENSPSSTTISGDKEALKEILARLKEEKPDVFARLLKVEMAYHSRASETLLLVLRSTHIFADHMKPLGIEYLELLRAENNFGIPRSADKALFLSSVICKPMVDTASFGPEYWVDNLNSPIWHQDSTATFLSALGRMYQEAVPLAVKALFPEERRALCGLPVYPWDHSESYWYESRLSSAWRQRPFPHHSLLGERTVDSPDFSPIWRNMLSLEDVTWIADHKIRRDVVFPLAGYIAMAGEAIRQTTGVDVGYRLRDVEARKALVLTDFKATEVVTVLHAHTGVPAEGPSWYDFSIASCHGGSWLIHCTGQVSPLAETLSLSWEPESASQRSLPRELTPMRFYEAMARVGVVFGPEFQRLVDITSSATDPLAEARVMSPAPRENKRPFALHPTAIDACIQLLIVAAARGLCRNLDQLEVPVVVGNVEVVSRGSANLRALAQDFTQGVECVTENSKLALRMSGLQLAPLAAEVADQIDVHAGARLQWLPDFDFVDHSGLFNKPQRPSLEIELEEQLTLLCVLESMDKVAHIPPCHPHLAKYREWLALQVRMAEAGQYTLVEHAKEWVGLPRVKRQKLIQDTYAKILDLPGRHAYSVGIKRICEHADQIFTGEADTLDLLMQDDILAELYDSVSFGYGDFVRLLSSNRPNLRILEVSAGTGGTTEQTLRGLVDSSSLPPYSVYTFTDVSAGFFPQAKERFAYAPNMEFRTFDISQDGLIQGFEAASYDIILAPNVVHATASLKETLSNLEPLLKPNGFIILTELCSLIKSPNYIFGNFVGWWLGEADGRDWEPYVQPEQWDAELKAAGFTGVNAVVPDQDVPSYRLAATIISQPARKADVPEIDRAVTLLYQSADADIVERLQLYLRDSGWQVDKCQLGEEMPPKGQDIIASVRLESDFFGEDLSPDRLAAFQALIRHLDAEKVLWLCPRFQVRCKDPQSAQTLGVAQTVRTELNLPLFTLEIDTNETRFEELVHRVLQKIRTTKDEASLHADKEFVVTDGQVCIGRYHPFDLKKELPSRALTLDSAAAYLLVGGTGGLGRSMTTWMVEYGATELILLSRRAGKDRESQSLSQELEQMGCSVHLVAGSVENPEDIARSIASAKKPIKGVFQLAMVLQDAPLLEMTYSDWVNVNGPKVTGTWNLHHALKDQPLDFFWLASSILTAVDTPGQANYLATGTFLEAFCQYRHSLGLPASVLNICPVEGVGFVAENAHAKKNMKAQGIYTLREREFLDFVELSLIDSTPSAGGNTSPTATPPGPWVNRSQVVMGLRSEQGLGDPHNRASWRHNRRMRLYHNRRTQESESARDGKNGAKASALQAFLERVREMGDEGLLRGEGVDFLAFEVGKKIHDLMLKPDEEVEIGRTLAQIGLDSLMAIELRRWIKQVFGLTMSVLEIMGSGSLKQLAEDLAAKYAEKIQQ