The following text describes a protein:
Function:
Required for the positioning and anchorage of the microtubule minus-ends in various cells. In fat body cells, part of perinuclear non-centrosomal microtubule-organizing centers (ncMTOCs) which function to accommodate the organization of microtubule (MT) networks to control nuclear positioning and dynein motor-based retrograde endosomal trafficking. Within the ncMTOCs, Msp300 and shot anchors the ncMTOC at the nuclear surface and recruits the MT minus-end regulators Patronin and Nin for assembly, anchoring and/or stabilization of circumferential and radial MTs at the ncMTOC. This protein may also function with Patronin to recruit msps to the ncMTOC for the gamma-tubulin-independent elongation of radial MTs. In embryonic myotubes and larval myofibers, functions with ens to regulate myonuclear positioning and, as a consequence, is involved in muscle development. Likely functions by positively regulating ens. Essential for embryogenesis, likely by contributing to accurate chromosome segregation during early embryonic nuclear divisions. However, other reports found that it is not essential for embryogenesis or embryonic cellular divisions.
Subcellular Localization:
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, perinuclear region. Cytoplasm. Note=During embryogenesis, localizes to the periphery of the centrosome where it partially overlaps with the pericentriolar region. Also localizes to pericentriolar region in larval brain neuroblasts (NBs). In dividing larval brain NBs and male germ stem cells (GSCs) asymmetrically localizes to centrosome pairs by preferentially accumulating at the younger, daughter centrosomes rather than the older, mother centrosomes. In NBs daughter centrosomes it is only detected at interphase or during early mitosis. However, it is symmetrically localized to centrosome pairs in embryos and ganglion mother cells in the developing brain. In wing epithelial cells, localizes to non-centrosomal microtubule-organizing centers (ncMTOCs) at adherens junctions near the apical membrane. In the fat body and myocytes of third-instar larvae, localizes to perinuclear ncMTOCs. Enriched at the plus-ends of growing microtubules (MTs). Binds MTs, and with Dynein can move along MTs toward their minus-ends. From stage 14 to stage 16 embryos, during which myonuclei split into two clusters and migrate toward muscle poles, broadly but weakly expressed in the cytoplasm and strongly enriched in cytoplasmic puncta of myotubes.
Sequence:
MEVSADPYEQKLYQMFRSCETQCGLLDEKSLLKLCSLLELRDQGSALIASLGGSHQLGVSFGQFKEALLNFLGSEFDGNTSSGFIERSLVITDEPLNNTYIESPPESSDREVSPKLVVGTKKYGRRSRPQQGIYELSVTDSDNTDEDQLQQQQNQRSLNGCDELGVQVQRSSSQSDLPGSRRLRSVHTSGSKLKRCASLPARRKMNSNTTGATTSPTAAAKLKQLSIQSQAQHSSSVESLDTVTPQQLETISVHSIMEAWELASIPNTRNLLHVLGFDEEEEVNLQQLTKALEEELRGIDGDHEQSNMLRALAALQATELGNYRLAYRQQHEENLKLRADNKAANQRVALLAVEVDERHASLEDNSKKQVQQLEQRHASMVREITLRMTNDRDHWTSMTGKLEAQLKSLEQEEIRLRTELELVRTENTELESEQQKAHIQITELLEQNIKLNQELAQTSSSIGGTPEHSPLRPRRHSEDKEEEMLQLMEKLAALQMENAQLRDKTDELTIEIESLNVELIRSKTKAKKQEKQEKQEDQESAATATKRRGDSPSKTHLTEESPRLGKQRKCTEGEQSDASNSGDWLALNSELQRSQSQDEELTSLRQRVAELEEELKAAKEGRSLTPESRSKELETSLEQMQRAYEDCEDYWQTKLSEERQLFEKERQIYEDEQHESDKKFTELMEKVREYEEQFSKDGRLSPIDERDMLEQQYSELEAEAAQLRSSSIQMLEEKAQEISSLQSEIEDLRQRLGESVEILTGACELTSESVAQLSAEAGKSPASSPISYLWLQSTIQEPAKSLADSKDEATASAIELLGGSPSHKTASRNNLTTSETSIFSTTPFESSQSGPSPTNSGNSNAYGANPGPAPISKPKRSQSPQQAAASEGEIADCETSSTASGKSFESNSKTSCLSHEKCSSPSALKEELKRLKFFELSLKEQIKDLSLQRDGLVMELQQLQEARPVLEKAYARTTHPTLQQRLNQLELRNRHLQNVIKQQQHYTESLMQQSWRQHQVELNDLHSRIETQGVLLADQTQRLQSADILVKDLYVENSHLTATVQRLEQQRARVNLIHQQQQQQRLVGGGLPGMP