The following text describes a protein:
Function:
Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the linear tetracyclic TAN-1612 neuropeptide Y receptor antagonist. The decaketide backbone of TAN-1612 is synthesized by the non-reducing polyketide synthase adaA via condensation of one acetyl-CoA starter unit with 9 malonyl-CoA units. The FAD-dependent monooxygenase adaC then performs hydroxylation at C2 while the polaketide chain is still attached to the NRPKS adaA. The alpha-hydroxylation step at C2 appears to be crucial for the following C18-C1 Claisen cyclization and release of the C9-hydroxyl version of TAN-1612 from the NRPKS adaA, two steps performed by the lactamase-like protein adaB. Finally, the O-methyltransferase adaD performs the C9 O-methylation to complete the biosynthesis of TAN-1612.
Domain:
Multidomain protein; including a starter unit:ACP transacylase (SAT) that selects the starter unit; a ketosynthase (KS) that catalyzes repeated decarboxylative condensation to elongate the polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects and transfers the extender unit malonyl-CoA; a product template (PT) domain that controls the immediate cyclization regioselectivity of the reactive polyketide backbone; and an acyl-carrier protein (ACP) that serves as the tether of the growing and completed polyketide via its phosphopantetheinyl arm.
Sequence:
MSGPTKLVFFGNEFPNDDLKALFRGLHRHGKDRRFRQLATFLEESTRVLQNEVAQLPEPLKKLVPHFENLMPLTEVDFRQGPLGAAMESALLTILELGMFIGHYEAEERVWDLSADRTTLAGLSIGLLAAAGVALSTHLAEVVQNGAECVRVSFRLGVYVHDISRKLEAPQADGSLLSWAHVVTGETASDLQEELSRYNTETGTPELLKVFISAADKTSVSVSGPPSRIRAAFRASQRLRYSKSLALPVYDGLCHAAHLYDEETIHRVLHPDGSVIPTSRPVQLALLSSRSGQPFEATTAAELFRAISTELLTGTIFLDNITAGILDRTERCADAPQCQIETYRTSLVFKGLLKALEACFPDRTISTTDLIPWVFQDYGARQPKSYADSKLAIVGMACRMPGGANDLDLFWELLAQGRDTHTTVPADRFDLETHYDPTGETENATRTPFGNFIDQPGLFDAGFFNMSPREAEQTDPMHRLALVTAYEALEMAGIVSGRTPSSNPKRIATFYGQASDDWRELNASQNIGTYAVPGGERAFANGRINYFFKFGGPSFNLDTACSSGLAAVQAACSALWAGEADTVLAGGLNIITDPDNYAGLGNGHFLSRTGQCKVWDQSADGYCRADGVGSVVIKRLEDAEADNDNILAVVLSAATNHSAEAISITHPHAGAQKENYTQVLHQAAVNPLDISYVELHGTGTQAGDAQEAESVLDIFAPRAHRRRADQPLHLGAVKSNIGHGEAAAGIASLLKVLLMYQKNEIPAHIGIPTVINPAIPTDLEQRQVYLPRTKTAWPRAAGQIRRAIVNSFGAHGGNTTLVLEDAPEKQVTVAREERSTHPVVISAKSKKSLAANVETLLAYLDENPETDLGDLSYTTCARRMHHSWRLATAVSDIPALQKFLRNAVSNDAVSQTRPIPTEAPHVVFTFTGQGAYYAGLAQGLFQALPFFRAEVRQLDHLSQRLGFPSIVPVILGEVEEGTATALVTQLSIVIVEIALARLWLLLLGIPAPHAVIGHSLGEYAALAVAGVLSTADALYLVGHRAQLIEEHCTPGSHAMLSVRATIADIERLVGTGADAPTYELSCQNTHQDTVIGGSIQDLNAIREKLEPEGIKCVNVDVPFAFHTAQMDAVRERLAKAVAAVPFKTPSVPVLSPLLGSVVFDGKSINPEYIVRATREPVRFATAIDAAQELGIVNSQTLWVDIGPHPICASFVRSLVPGARIVSSCRRNEDNFATMAKSLCTLHLAGRTPSWAEYFRPDEQAYSLLRLPKYRWNEVNYWIQYLGTWTLDKAHLKNGGSQKRTITDVPSVSSLRTSLIHQVTEETVDKTTATLKAISDIQHPDFLEAVHGHTMNNCGVATSSIWTDMAMTVGEHLYRRLVPGTDHVLMDLCDFEVQHAQVANTNSNTPQPLALEAHLDLPTRHMSLAWYDVNATTNQRADAPFATGSIKYPADPTGAAWSIEWSRITHLIQGRIEALQHLAAENKASTLSKPLAYALFKNVVDYAPRYRGMDRVVIHDHEAFSDITLTTDRHGTWHTPPHWIDSVSHLAGLVMNGSDASNTRDFFYVTPGCGSCRMTEPLIAGGKYRNYVRMFPMPDEAHMYAGDLYILREDKIIGVVEQLKFRRVPRLLMDRFFSPNKNAAAHAAPAPAPAAVPAVKKQPPTETIQPQAPKTEQKQDQLQLPNLASAAPSTANSSSSPSSSGVATPTTEQEAPVADASAVTGVAGKCLELIANETGLGVAELTADATFVQLGVDSLMSLVLSEKLRSEMGLEIKSSLFLECPTVGDLTGWLEQYC