lhallee/plat_data huggingface dataset:
The following text describes a protein:
Function:
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
Subcellular Localization:
Nucleus.
Sequence:
MVAAVSVPSQAQAMLRGRLCDPAFVHSALRSSPDTNYSKLKYLVSSSVSEACNNSVLLLGPRGCGKAAVVDMVLDDLNKEHPDAISVIRLNGMLHSDDNCAMKEIARQLCLEHQLSFSKMASSDDNTEFMIDMLRECGLAHKTILFVLEEFDLFAQGKQRLLYSLLDAMQSLTSQAVVIGVSCRLDADQLLEKRVRSRFSHRKLLFISPSLDDIQRLVEHLLILAKDSGLPEKYITDYNSRLTSIFSDKKFKGCLNSLMDADATTSNILRFLFRAVSYMDMESGFLPIESFLKALSSMQRQPKMDSLQDLSILELYILVCMHRLEDKEQSSYNFTSIMKEYRSIQDAYKTSDKYASTVCFRAFEHLLDRELISFGDNRGRNQALEYRPVKLLVSSRELAQSLKLNTTCPAVLQKLFDRERYM
The following text describes a protein:
Function:
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6/CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Subcellular Localization:
Cytoplasm.
Sequence:
MCQVQGDDTLMIEEVKILKRSSTCLKCDEAAVVIARVKDAFCRHCFLGYVTHRFKATIGKSRLIRDKEKVLIAFSGGQNSSAVARLIHEGRSENQHKRSDSSQVSSTDDGIITQQTPEERRANRETITKLMNAYDLPTCVVPIEEVFSLRLSAESTLRVKDEDDVTMAMEHFSQLSIQDEASQRLQDILDSVKSLTAKEDLVKTLRHRLLADIAKQTGHTKVMLGDNGTNLASRVLSGMAQGRGVTVPLEVGFADNREGDSTFVRPLREFPSREVSLYNHLMGVESVCIPTLTTKASQYASINKLTDDFIHGLQVGFPSTVSTVLRTGEKLCMVSSSGDEGSCIMCKAPLDTNVSACSALESTQHSLAELSDPDRGQQTANGQCSLAVSCETSYDDQSSSECCGQGDGSCHSTKKTISFGDVSKGLCYGCRITVRDMKGVNSLPGYVLEEAGRRQRRSEMKEEIQDFLLTDDR
The following text describes a protein:
Function:
mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria.
Subcellular Localization:
Cytoplasm.
Sequence:
MAKNKKQNGKAKTPPVVAAAAGEQKETIGNGHAEQNGLNGHASSEESDPAAAGPNESADDKSAGVAGGGETEQQQQQQEDDVMRLMQETGFTVQVLSPGVEPLSIQVSSMELVQEIHQLLMDREDTCHRTCFSLQLDGRTLDNFAELKNIDGLQEGSVIRVVEEPYTMREARIHVRHVRDLLKSLDPADAYNGVDCSSLTFLHTITMGDIMEKKKTRQESVDCTPPDFIMPGARERPLLPLQPGTGKKGTPQPLKVLTTSAWNPPPGPRKLHGDLMYLYVVTMEDKRLHISACSRGFYVNQSTDDAFNPQPANPSYLSHSLIDLLSQISATFRRCFAQMQKKRTQRHPFERVATPYQVYTWTAPALEHTIDAIRAEDTFSSKLGYEEHIPGQTRDWNEELQTTRELPRATLPERLLRERAIFKVHSDFVTAATRGAMAVIDGNVMPINPGEDAKTQMFIWNNIFFSLGFDVRDHYKELGGDAAAFVAPRNDLHGVRVYSAVDVEGLYTLGTVVIDYRGYRVTAQSIIPGILEREQDQSVVYGSIDFGKTVLSHPKYLELLNAAGKHLKILPHSVYNDKEEAIELCSSVECKGIIGNDGRHYILDLLRTFPPDVNFLALPAEEEAVGKESRAMGFPIEHRHKLCCLRQELLEAFVENRYLMFMKHAAVQLQQCVKMKQEQKAAAAQKTEEGGKQAAIEAAAPAEGDKTPAKDAKDGKEAGKDANDGKEEGSTTKEAAAAAAAARSVPKPDSEDAKKLVESLISSDQKNESMEVVKRACEAVGSLKEYEFNIRFNPDVYSPGIRHVDEEPNAAGSLRRQKQLVKDAAEFLVKHQIPSFVHECLDHTSAPMDGVTLTELLHNRGINVRYLGKVVDQLAKIKQLEYLHTIAVSELIVRAAKHLFTAYLQQTDVMSMAAAISHFLNCFLTVSTGGYQPVANGTGADGDGQLADEFGPKAGGKKQNKQSKRGGGGGGGKGAAGGGRKATFSVPSSDNCEWTALTSKTLWAQIRQELKAYWDFELTVEQPAKEGKESKAAVIDSIEPLIGAFKLQKISLLRSFCLKTGVQILLQEYAFEQRNRPAFTDADIVNVFPVVKHINPRASDAYNFYTTGQTKIQQGYLQDGYGLISEALNLLNNVYGAMHPENAQCLRMLARLSYIMGDPQEALAIQQRAVLMSERVNGVDHPYTISEYGHLALYCFANSQITTALKLLYRARYLATIVCGENHPDIALMDSNISLILHAVGEYELSLRFLEHALALNIRYYGEKSLKVAVSYHLVARTQSCMGDFRSALVNEKETYAIYKQQLGENHEKTQESSECLRHLTQQAVVLQKKMNYANGKLLSTGLPPIHIQPPSMGSVLDMLNAINGIIFVQISSKEIANFKNEIEKRQKEAGAQSQQPGGGPVQANQEEVDQMLMETMQKTAAGIPFEEQDGEKKDGAAEAASHTAGGAAANTAAPAVSPRRKS
The following text describes a protein:
Function:
Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion; Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.
Subcellular Localization:
Virion membrane; Single-pass type II membrane protein. Host cell membrane; Single-pass type II membrane protein. Note=Folded in the endoplasmic reticulum.
Domain:
The C-terminus (head domain) is involved in binding the cellular receptor.
Sequence:
MDGDRGKRDSYWSTSPSGSTTKPASGWERSSKADTWLLILSFTQWALSIATVIICIIISARQGYSMKEYSMTVEALNMSSREVKESLTSLIRQEVIARAVNIQSSVQTGIPVLLNKNSRDVIQMIDKSCSRQELTQHCESTIAVHHADGIAPLEPHSFWRCPVGEPYLSSDPEISLLPGPSLLSGSTTISGCVRLPSLSIGEAIYAYSSNLITQGCADIGKSYQVLQLGYISLNSDMFPDLNPVVSHTYDINDNRKSCSVVATGTRGYQLCSMPTVDERTDYSSDGIEDLVLDVLDLKGRTKSHRYRNSEVDLDHPFSALYPSVGNGIATEGSLIFLGYGGLTTPLQGDTKCRTQGCQQVSQDTCNEALKITWLGGKQVVSVIIQVNDYLSERPKIRVTTIPITQNYLGAEGRLLKLGDRVYIYTRSSGWHSQLQIGVLDVSHPLTINWTPHEALSRPGNKECNWYNKCPKECISGVYTDAYPLSPDAANVATVTLYANTSRVNPTIMYSNTTNIINMLRIKDVQLEAAYTTTSCITHFGKGYCFHIIEINQKSLNTLQPMLFKTSIPKLCKAES
The following text describes a protein:
Function:
Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Exerts distinct biological functions in the mammary epithelial cells and T cells in a tissue-specific manner. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Cross-antagonism between ZBTB7B and CBF complexes are determinative to CD4 versus CD8 cell fate decision. Suppresses RUNX3 expression and imposes CD4+ lineage fate by inducing the SOCS suppressors of cytokine signaling. induces, as a transcriptional activator, SOCS genes expression which represses RUNX3 expression and promotes the CD4+ lineage fate. During CD4 lineage commitment, associates with multiple sites at the CD8 locus, acting as a negative regulator of the CD8 promoter and enhancers by epigenetic silencing through the recruitment of class II histone deacetylases, such as HDAC4 and HDAC5, to these loci. Regulates the development of IL17-producing CD1d-restricted naural killer (NK) T cells. Also functions as an important metabolic regulator in the lactating mammary glands. Critical feed-forward regulator of insulin signaling in mammary gland lactation, directly regulates expression of insulin receptor substrate-1 (IRS-1) and insulin-induced Akt-mTOR-SREBP signaling. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes. Potent driver of brown fat development, thermogenesis and cold-induced beige fat formation. Recruits the brown fat lncRNA 1 (Blnc1):HNRNPU ribonucleoprotein complex to activate thermogenic gene expression in brown and beige adipocytes.
Subcellular Localization:
Nucleus.
Sequence:
MGSPEDDLIGIPFPDHSSELLSCLNEQRQLGHLCDLTIRTQGLEYRTHRAVLAACSHYFKKLFTEGGGGTVMGTGGGGTASGGAGAGVCELDFVGPEALGALLEFAYTATLTTSSANMPAVLQAARLLEIPCVIAACMEILQGSGLEAPSPDEDDCERARQYLEAFATATTTASTSGMPNGEDSPPQVPLLPPPPPPPRPVARRSRKPRKAFLQTKGARANHLVPEAPTVLTHPLTYEEEEMVGRLGNSGGSGLGDSYSPPTGAASPAEGPLNYEVFEGEEEEEEMAYPPGYGLAQSNEPSLSPEELGSDEDPIDPDLMAYLSSLHQDALTPGLDGQDKLVRKRRSQMPQECPVCHKIIHGAGKLPRHMRTHTGEKPFACEVCGVRFTRNDKLKIHMRKHTGERPYSCPHCPARFLHSYDLKNHMHLHTGDRPYECHLCHKAFAKEDHLQRHLKGQNCLEVRTRRRRKDDVAAPHYPPPSTTTSSPAGLDLSNGHLDTFHLSLARFWEQSATTGPPVTTQGPPEEEEEEGTPTTPQAEGAMESS
The following text describes a protein:
Function:
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Subcellular Localization:
Golgi apparatus membrane; Single-pass type I membrane protein.
Sequence:
MQMNSVWKLSLGLLLLSSVIGSFAELDFGHCETLVKKWADSSSSREEHVNKDKRSLKDLLFFLHVPRTGGRTYFHCFLRKLYDSSEECPRSYDKLHFNPRKEKCKLLATHDDYSLMAKLPRERTSVMTIVRDPIARVLSTYEFSVEVAARFLVHPNLTSASRMSSRIRKSNVISTLDIWPWKYLVPWMREDLFARRDARKLKEVVIIEDDNPYDMEEMLMPLHKYLDAPTAHDIIHNGATFQIAGLTNNSHLSEAHEVRHCVQKFKSLGESVLQVAKRRLDSMLYVGLTEEHRESASLFANVVGSQVLSQVVPSNATAKIKALKSEASVTISETGSDKSNIQNGTSEVTLNKAEAKSGNMTVKTLMEVYEGCITHLRKSQGTRRVNSLKRITPANFTRGTRTRVPKEVIQQIKSLNNLDVELYKYAKVIFAKEHELVSNKLISSSKRSIVDLPSELKSVLGEMGEEKLWKFVPVALMLLLIVLFFLFVNAKRRRTSKVKI
The following text describes a protein:
Function:
Catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-ethanolamine branch of the Kennedy pathway. Also exhibits choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway, but its preferred substrate is ethanolamine.
Miscellaneous:
MISCELLANEOUS: Present with 3420 molecules/cell in log phase SD medium.
Subcellular Localization:
Cytoplasm.
Sequence:
MYTNYSLTSSDAMPRTYLVGTASPEMSKKKRQSANCDKPTRRVIHIIDTNEHSEVDLKNELPITCTNEDGEMTSSSWTSQTANDFLKLAYVNAKLDPSLPSQYFKQDIINVLQSLEIPGWSVPGSKESSLNKNLLTLTQIKGALTNVIYKIHYPNLPPLLMRIFGDSIDSVIDREYELKVIARLSFYDLGPKLEGFFENGRFEKYIEGSRTSTQADFIDRDTSIKIAKKLKELHCTVPLTHKEITDQPSCWTTFDQWIKLIDSHKEWVSNNVNISENLRCSSWNFFLKSFKNYKRWLYNDSAFTSKLLREDDKDSMINSGLKMVFCHNDLQHGNLLFKSKGKDDISVGDLTIIDFEYAGPNPVVFDLSNHLNEWMQDYNDVQSFKSHIDKYPKEEDILVFAQSYINHMNENHVKIASQEVRILYNLIIEWRPCTQLFWCLWALLQSGRLPQRPLIEGEKLMSEKAGLGDETHLMEHKNKENGKYDCSEDDSFNYLGFCKEKMSVFWGDLITLGVIDKDCPDIGKTDYLDTKLIF
The following text describes a protein:
Function:
Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure. Specifically recognizes and binds proteins with a RFXV motif. Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux. Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities (By similarity). Phosphorylates RELT (By similarity).
Subcellular Localization:
Cytoplasm. Nucleus. Note=Nucleus when caspase-cleaved.
Domain:
PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.
Sequence:
MAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS
The following text describes a protein:
Function:
RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Negative regulator of poly(A) synthesis. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. REF2 is required for 3'-end formation of snoRNAs.
Miscellaneous:
MISCELLANEOUS: Present with 7450 molecules/cell in log phase SD medium.
Subcellular Localization:
Nucleus.
Sequence:
MSAPVPQLVNISHALQASTIQQIRLDMVDFNKDCKLSSIQLARIDKYIDSLQAALNQFTKDNLHIERKEKNVTEADIQLYSGLKSMYLDYLNQLIKLKHEKQHHSTPPIANDVSLDFFVNQLPKFSPEERKNYIDNLILNKNSHNRLSKMDGLVDAVINLCVLDTSVAENVRSYMKLLDTLGFQKGSNSTGTKANLKKKLASSKAKIKDSEKEKEKEKDKSKVKMKTKLKPSPLLNNDDKNSSPSPTASTSSMKKLKSGLFNKNEAKSTESLPTSSKKKLSFSKYLNKDDADMTKLGTKRSIDVDFKVNPEASTVASNIISSSTSGSSTTTVATPASSEEPLKKKTKISVQDSNVQSILRNGKPKKARISSIKFLDDSQLIKVYGDDLPNQGLQVSPTQLKKILKPFKEGEPKEIILFEDMSIKLKPLDLMFLKNTNSDDYMDISETKGGPIHCETRTPLIYRKNFNHFNPDLNKRPPREPIEFDLNGNTNSTPTIAKAFGKNSLLLRKDRGGLPYKHVPIVKRNKYPPRPVH
The following text describes a protein:
Function:
Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Subcellular Localization:
Cytoplasm, cytosol.
Sequence:
MVEPVKLIGGFGSPFVHRAEVALRLKGVPYELILEDMTNKSELLLKHNPVHKKVHVLLHGDKAVCESLLIVEYVDEAFDGPPILSTDPHERSEARFWSKFFVGKLALWTEEGEDQKVFVMDAKENLALVEAQLSQKRFFGGATIGLADIAGVSLLSRWASVMQGVAGVSVMTDDEYAAIHRWMEDYNADEALRSACRTETISSPTSPRSGESASPRPSPCYPII
The following text describes a protein:
Function:
Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.
Subcellular Localization:
Nucleus.
Sequence:
MATTTGLESLVDQIISVITNDGRNIVGVLKGFDQATNIILDESHERVFSTKVVLVQLCSLSEHPSSSGIGHLISSFTFLSFDMLFCLKHEGVQQHVLGLYIIRGDNIGVIGELDEELDASLDFSKLRAHPLKPVVH
The following text describes a protein:
Function:
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins (By similarity).
Subcellular Localization:
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence:
MLLDDQLKYWVLLPISIVMVLTGVLKQYIMTLITGSSANEAQPRVKLTEWQYLQWAQLLIGNGGNLSSDAFAAKKEFLVKDLTEERHLAKAKQQDGSQAGEVPNPFNDPSMSNAMMNMAKGNMASFIPQTIIMWWVNHFFAGFILMQLPFPLTAKFKEMLQTGIICQDLDVRWVSSISWYFISVLGLNPVYNLIGLNDQDMGIQAGIGGPQGPQGPPQSQVDKAMHAMANDLTIIQHETCLDNVEQRVLKQYM
The following text describes a protein:
Function:
Involved in ubiquitin-mediated protein degradation. Regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates. Targets proteasomes to the nucleus and facilitates the degradation of nuclear proteins (By similarity).
Subcellular Localization:
Cytoplasm. Nucleus.
Sequence:
MMSTNFQWPGTNKNDNTEVSVETPSSTDPHVPRYPFTAMSHATASTTMKKRKRDDFDGDKSTTITMNTTTTRKYIQSSLGSSKFKKAKTPKISGQPLPLPRLIESLDKSNLQKLVQDLITVHPELQSTLIKISPRPSIQDSIQLLQDKFDMIISHLPYKCDVESDYSYLRIKPHLQEFLSSVSDFILNYLPPLETNMTHSLQFLHETTKLVYNLPNFTNQEFQYTKSSALEQIANCWLIVLSQDEEKEGNTDVVKVIQELELLEKLHEHNEISFNKFEKVVDYCKDKLEQHELIMNNNEAGSGVTSSISDLITVDYSKYSIANTTSI
The following text describes a protein:
Function:
Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation.
Subcellular Localization:
Cytoplasm.
Domain:
In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
Sequence:
MGKGCKVVVCGMASVGKTAILEQLLYGSHTVGAETSDTQEDIYVASVETDRGVREQLRLYDTRGLREGLDLPKHFFSVADGFVLVYSVDCLESFKKVEVLKKEIDRSRDKKEVMVMVLGNKCELRERRQVDQDTAQQWARGEKVKLWEVTVTDRSTLIEPFTSLTSRLTQPQSKSAFPLPGRKSKGTPSNDI
The following text describes a protein:
Function:
In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.
Sequence:
MCCNYYRNCCGGCGYGSGWSSGCGYGCGYGCGYGSGCRYGSGYGTGCGYGCGYGSGCGYGCGYSSSCCGYRPLCYRRCYSSCY
The following text describes a protein:
Function:
May play a role in acrosome formation in spermatogenesis and in fertilization.
Subcellular Localization:
Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass membrane protein. Note=Exclusively localized on a peripheral part of acrosome membrane (Golgi phase), localized on the equatorial segment of the acrosome (maturation phase).
Sequence:
MQAMDGEVLLPALYEEEEEEEEEEEEVEEEQVEKGGSLGSLSMGKHRGLSLTETELEELRAQVLQLVAELEETRELAGQHEDDSLELQGLLEDERLASAQQAEVFTKQIQQLQGELQHLREEISLLEHEKESELKEMEQELHLAQAEIQNLRQAAADSATEHESDIASLQDDLCRLQNDLDDMERIRGDYEMEIASLRAEMELKTSEPSNLSISDFSGIQDELHHLRERYNLLNEEYQALRESNSSLTGQLAELESDRTRRATERWLESHLLRSTMSSESQTSELDFPEPDPVMQLLRQQLLGAEEQMQDMQDKCKNLYCELEELQHHRRTSEEEQKRLQRELKCAQNEVLRFQTSHSTQQHEELKSRLCTLQQKYDASQDEHSELLKVQMQLETELQQLRLLRCTPVESQSEKELMCRLQKLQAQHQCSVNEKEQLLEVQHHLHDKLRCHESEVHRLRSMVDCLREKNEKNSGIHLQLQEMKGLYQFSRDELERQKHMYDQLEQDFLLCQQELTELKSSQSLCEENGNCSNKCDALLARLTELQDKFKASQEEIGHLQMEQCELLEDQRRLQEEQGQLQEELHRLTFPQPKCGILQKSQELLSKLQDLCEMQLLYQNMQEQQRKLTQNQECVLKEQLEAHKHLRGFKESHFQEVLANPQDARGPKSSSCENKFKVLMDQLQALQVLYDTSQKQQEVLQREHGRLMEERKRLQAELQLCMEEMQVLQTQSPMIKRSFEYCGKNSGSRAPSTENFHRSYESSIDENEGYQKSYVSSQPSTETFLKSYDSSTSANEAFEKSYCSSSTSVSYKKSYGSVSSGETLHRSYASSSTDEDPAEPEDLEHFEETVAKVLTKLQAVKALYQVSQEEHCQLQQRMHRLLAKQKELTEELQCCEKELRECMESLGKPLPPQSDKCEIKELQTKLRELQLQYQASMDEQGRLLAVQEQLEGQLQCCQEELRQLKENRPSISSEARGKNVNKNMNKNANGVRNKKLSMACSEDLENGFENEKNLEVMLYYKASQRRLDELMKEEKEIEEARKKEREKKAKKDLCKLATNPAADPRAEPEPTEDEEENFEEYREGEDESCEAAEEGNPLKLSESKKPSPAPDPPIFSLPLVGLVVISALLWCWWAETSS
The following text describes a protein:
Function:
Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in endocytosis.
Subcellular Localization:
Membrane; Peripheral membrane protein.
Sequence:
MEPPAAGNLLEIMDLARTLRQEQLFIQQEQAAFAQLTGAFESNAGTITKLAFVCAQQRQILNELLLARTDQDPLLFCRRASAYDSAQFVDAKQLLPYEHALAYEDLFNYLYNTPYLLALSLATADRLSLLSASQLGQIINTIATGLYGNAINTKDVELLLKLLRELIEIQLLTSEQPRRLLRTNSSSFARLYQRLVESLFSARIFLTAALHAPLMGVLSEHEIWLDLDPHKLMQTFTPKEREKRFGREGDEEYQRNVARFHAETLGKLHSHVQEFVKSLQQSWALFPSSLRWLLQTLSQQLRQSLRHEEQEIRQLLTDLVFTHFISPAIASADLLGIIDVNVSERMRHNLNQIVKLLQRLALNDEDSELVQLMELLMLGQTGEDVVAILPQQSDFERSQLAINQRELAQLVEFFRLLTARDEYDISVEERQRLQRILGRIPKQQVQQQQQTPKDAPDSRESPEKSKKSNKSLMSLGKAKKKLAKGMSFSSGSSNHNPVPVSEPLTNGQANTSNGSGGLGADLEHCSSHSGSNTSLSSCGANMPTTNDPLDMSTSSDPVLVFSLYNAGAKSKLKPLTEEEVLKMNSIGQDGSNLLPAVVTTAPLAPSSNNDDVSSLEAMRRPQDDASIGNSDNLEAISEAAHSVASSLDLEEQQERDVHENEDNLSDMVSANVSGRGTPNISGRDTPSSQVTDGDGAGGDLGHHHGVHARAAANPQMQKMLLSKARSDIEDKFCKFELKKFEGDENVSIISDTWSTDVLASDSENTLDATVSERGDRDRNFSTPLIPSAVVLPGDNNFVVEALARAGRVSGVHGPQLDASDQRSESNWSTDVLASDSEKLAEIDTDDNVSITTKSDTTAPQAAVLDDDDEDEQTPGSSGDGEPDPDRGNGSERSQEDSAFFDAVNSYEDANLYHGASSLARSSVRTSYHVLGGESSFQQQYKCSGADSSGRKTTPLMGTSCMRRQTSAESSISNQSLNLEEPPPPMGKHHHHHREHHHRDYHHHHRERERERERERSALKKKKHQQQEKEHRDLIDFSDCSEDKEELARNRDEEQPPGLVQQLLDMINQDEQTGAVSSSSPNVEHRRISIEQRSAIIDGRRNGILAGSMRRHQSLNYENHEIMLNSMLPKTDDDKQEMLLCVQTQQLQLEERRGSAGLRPEVDGASGIAAAGGSGLKPPTKATGAIPKSISFDASADKDKQTYHRDGERDRERDRERERDRERDRERDRERDRERDHHGAGIFNKLRQGIFKNRRGASAKNASNSSNQSNPAASTSSVQADIRSVSFDPSAGCDNFGTHYCDTSEDILAKYRRKVSSSSEATNSDSTGNGHGVGSTGSVGAAAHLHKHMNGGHIPGVVFGCVKQKLRTVLSRTDLHSGDFRQTSTTSTTMATPLQIYLQIQLAQCISLQRLPQISHVAEALRCLAQLERPQHGQLLAELQRDLERRQSYLQYLMRHRQQLLLRSEQLEQLEARLRGEARSSQRCLLQALVRMYLAWSRQQEKLEQFQAEFAQLRASDERVELTEEFVESLLQELRSSADLQDEWQVDAARVAIERMLLEQMYEQVMFPNEDADVSRDEVLSAHIGKLQRFVHPAHPALCIAQEYLGEAPWTFAQQQLCHMAAYKTPREKLQCIINCISSIMSLLRMSSGRVPAADDLLPVLIYVVIMANPPYLLSTVEYISCFLGKKLEGEDEFYWTLFGSVVKFIKTMDYLD
The following text describes a protein:
Function:
TIR-NB-LRR receptor-like protein that confers resistance to the pathogens Leptosphaeria maculans (blackleg disease), Botrytis cinerea, Alternaria brassicicola and Alternaria brassicae. Required for efficient callose deposition downstream of RLM1 during infection with L.maculans.
Domain:
The TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.
Sequence:
MKSSSSQSYDVFPNFRGEDVRHSLVSHLRKELDRKFINTFNDNRIERSRKITPELLLAIENSRISLVVFSKNYASSTWCLDELVKIQECYEKLDQMVIPIFYKVDPSHVRKQTGEFGMVFGETCKGRTENEKRKWMRALAEVAHLAGEDLRNWRSEAEMLENIAKDVSNKLFPPSNNFSDFVGIEAHIEALISMLRFDSKKARMIGICGPSETGKTTIGRALYSRLKSDFHHRAFVAYKRKIRSDYDQKLYWEEQFLSEILCQKDIKIEECGAVEQRLKHTKVLIVLDDVDDIELLKTLVGRIRWFGSESKIVVITQKRELLKAHNIAHVYEVGFPSEELAHQMFCRYAFGKNSPPHGFNELADEAAKIAGNRPKALKYVGSSFRRLDKEQWVKMLSEFRSNGNKLKISYDELDGKGQDYVACLTNGSNSQVKAEWIHLALGVSILLNIRSDGTTILKHLSYNRSMAQQAKIWWYENLERVCKKYNICGIDSSTDGGGSTYGQCSNSQFQRNMDASPGGNKTSNQSTKDSPRASQVEKEKIEYCEPHVYITPAIFSDGTRAPKYVESSSRRVTQVHHAKTWWPENCEKVYENHNNIYGIDRSIDGGDKFEGKSKVSDGGLDGKDQGSMYGQSSNSELQINMDADNRRCEPVSEMLFKNYNVCSPNGLTDVNCSNPQSQRKLDASLKKDKIVHEWIRTGSGFFFDFQGPKSIVSAAQVDEKNFEYCEQGVYITLGILSGGIIVLKHLEFSRRMAQQAKVWWSENWIKVYQEHNICGIDKSFDGRFDDRRVIRQLRPN
The following text describes a protein:
Function:
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released. Responds to heat shock and surface stress (detergent exposure). When combined with isolated core RNA polymerase from M.smegmatis is able to guide initiation from the sigB promoter. Required for full expression of sigB, and for sigB induction after detergent exposure but not after heat shock. Controls a regulon of about 38 genes in culture and about 16 genes during macrophage infection , most of which have decreased expression in a disruption mutant. Probably down regulates the host immune response to mycobacterial infection.
Miscellaneous:
MISCELLANEOUS: [Isoform 1]: Produced following surface stress; MISCELLANEOUS: [Isoform 2]: Produced following oxidative stress and heat shock. Shown by mutagenesis of the start codon and subsequent loss of translationally fused LacZ; MISCELLANEOUS: [Isoform 3]: Produced following oxidative stress and heat shock. Able to interact with RseA. Shown by mutagenesis of the start codon and subsequent loss of translationally fused LacZ.
Domain:
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity); The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (Probable).
Sequence:
MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWVEPSDQLQGTAVFDATGDKATMPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMEALPEDYDRVPADEPNPEQIYHDARLGPDLQAALASLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQALRDYLAAHPEHGECAVHVNPVR
The following text describes a protein:
Function:
Antitoxin component of a type II toxin-antitoxin (TA) system.
Sequence:
MDLSRVSFYSLADAKARFSQVVEEAKTKDVVVTKNGVPAVAVIDYEKYKKLMEFMDEILDTYLLDIGNVEKYLELKRYFEFNDSQEV