lhallee/plat_data huggingface dataset:
The following text describes a protein:
Function:
Involved in control of chromosome replication initiation. Inhibits the cooperative binding of DnaA to the oriC region, thus negatively regulating initiation of chromosome replication. Inhibits the ability of DnaA-ATP to form a helix on DNA; does not disassemble preformed DnaA-DNA helices. Decreases the residence time of DnaA on the chromosome at its binding sites (oriC, replication forks and promoter-binding sites). Tethers DnaA to the replication machinery via the DNA polymerase beta sliding clamp subunit (dnaN). Associates with oriC and other DnaA targets on the chromosome in a DnaA-dependent manner.
Subcellular Localization:
Cytoplasm, nucleoid. Note=Localizes in tight foci, which correspond to the replisome at mid-cell throughout the cell cycle.
Sequence:
MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLENSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELLYRE
The following text describes a protein:
Function:
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit recognizes the wild-type Chi sequence, and when added to isolated RecB increases its ATP-dependent helicase processivity.
Miscellaneous:
MISCELLANEOUS: In the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'-3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site.
Sequence:
MHATSAPDFRLYPSNALDTLAALLAEELRRPVPEQPVLQPEVVLIPQVAMRRWLQSTLAAEHGVAANLEFLTPGEFVARALERNLGPADDDLDMATTQWRLYQTLQGELGSDAALAPLAGYLADGDALKPWALAGELGSVFEKYQAWRRDWLLRWESGADADDPQARLWRSIAGGRQYRARRIGQYLDRYARPDGPLPQGLPKRLFAFAILNVSPDVLRVLATQARVGTLHFYLPTPTQGYWGDLQTLWQRRREGGAVALFAEQVQENPLLQAWGAAGRDFMALVGDYEVVHPLAEIAAYADPLDAGRRTLAEGGLGDSLLRRMQSDLFHRHAPAVPPVLPAVNLHDPSLQVHACHTRLRELQVLHDQLRALLDDARFDPPLQPREIAVLSPDIDPYVPYLDAVFGGHGSDDGLPYALADASPLASEPLADVFLTLLGLPISRFGLHEILDLLASAPIAEAAGLDEAGLERLRGWLHGAGARWGLDAVHRRQHQAPGDDAYTWRFALDRLLLGHASGAEDDIDGVAPWPQLEGSALAALDTLLRLLRVLDRHQAALAEAMTPVQWRECLLGLLEALIPAAPSAPRAQRALERLRTLIDQFARDAVRAEYAGNVPAEVVRAHFAAVLGESDTRAPLLTGGISFGRMVPMRLLPFRAICLLGMNDGDFPRRDPAAGLNRLTAELGTERRRHGDRSTREDDRFLFLQLFASAQEVFYLSYLGADARDGTVREPSVLVSELLGSAAQYHADPKAIDALVVRHPLQPFAAAAFGAVGEDGADPRRFSYRRQWRPAVDSLAGQRQPLAPWVAGALPADASVLPASVSIDDLRRLFADPAGQFLRHRLGMRLPDPAGEDSDLEPLLAPTRGLEQYGLQQQVFEAALAGDADGLYERLRARALLPSGPLGRRQLDERLRQLRPYADVFRQWRGEAPAQSQRLQVEIDGTNVHGRVPGWYANGVGRVQVGALSGRSAIRDGLEWLLLRAAGERVPFVRFFEHDDSLGPHPIDPEPLSQTQARAALGELLQLYRQGLQTPLAFAPYSSWKYHQAARNDELDKAIKDAHGQWQSSFGWSESHSPELRLVTRGRDPFGDAQQFVDFARTSHQLFALLEDGSAPAPLDPARVIESWRQWRGAQDDAE
The following text describes a protein:
Function:
Transcription factor which binds to RE2 sequence elements in the MYOD1 enhancer.
Subcellular Localization:
Nucleus.
Domain:
The CR1 and CR2 motifs mediate sequence-specific DNA binding, and are important for binding to the MYOD1 enhancer.
Sequence:
MQARRLAKRPSLGSRRGGAAPAPAPEAAALGLPPPGPSPAAAPGSWRPPLPPPRGTGPSRAAAASSPVLLLLGEEDEDEEGAGRRRRTRGRVTEKPRGVAEEEDDDEEEDEEVVVEVVDGDEDDEDAEERFVPLGPGRALPKGPARGAVKVGSFKREMTFTFQSEDFRRDSSKKPSHHLFPLAMEEDVRTADTKKTSRVLDQEKETRSVCLLEQKRKVVSSNIDVPPARKSSEELDMDKVTAAMVLTSLSTSPLVRSPPVRPNEGLSGSWKEGAPSSSSSSGYWSWSAPSDQSNPSTPSPPLSADSFKPFRSPAPPDDGIDEADASNLLFDEPIPRKRKNSMKVMFKCLWKSCGKVLNTAAGIQKHIRAVHLGRVGESDCSDGEEDFYYTEIKLNTDATAEGLNTVAPVSPSQSLASAPAFPIPDSSRTETPCAKTDTKLVTPLSRSAPTTLYLVHTDHAYQATPPVTIPGSAKFTPNGSSFSISWQSPPVTFTGVPVSPPHHPTAGSGEQRQHAHTALSSPPRGTVTLRKPRGEGKKCRKVYGMENRDMWCTACRWKKACQRFID
The following text describes a protein:
Function:
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Subcellular Localization:
Mitochondrion inner membrane.
Sequence:
MTHALPKVVKSQLVQDIGVALILGSIAGCFFKYGVDKKKQRERVAFYEKYDKEDL
The following text describes a protein:
Function:
A terminal oxidase that produces a proton motive force by the vectorial transfer of protons across the inner membrane. It is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at low aeration. Generates a proton motive force using protons and electrons from opposite sides of the membrane to generate H(2)O, transferring 1 proton/electron.
Subcellular Localization:
Cell inner membrane; Multi-pass membrane protein. Note=The displayed topology is based on not the large scale studies.
Sequence:
MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDTERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPVGFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGNFFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALAGVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLPLLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQLTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY
The following text describes a protein:
Function:
Facilitates the interaction of SYP31 and CDC48A, thereby regulating an CDC48A membrane-associated function (Ref.8). Appears to act as a negative regulator mediating the powdery mildew-plant interaction.
Subcellular Localization:
Membrane; Peripheral membrane protein.
Domain:
PUB (PUG) domain is required for the interaction with CDC48A (Ref.8). PUB domain may serve as a protein-protein interaction domain implicated in the ubiquitin-proteasome pathway.
Sequence:
MDDVKDKLKGFMKKVNLSSSSGKFKGQGRVLGSSSSSSAPPVNPIQNRFNSSQAPNPTPRPKPNPNPLPEKPLSSSDQKISGSTRNPDHDPVRAPQDGFDPYGAFITSSNRSQNGYSLSMFECPICKNPFTSEEEVSVHVESCLGDTNGDESSFEKDNNDNDKSEMEKLVVVYLSGKPSESSIDVLLRLFKNIVKEPENAKFRKVRMSNAKIKEAIGDVAGGVELLELVGFELKEENDEIWAVMDVPSEEQSILINKVVGYLEKRKTESSGSSAQVMEPVAPKKIDREIRVFFSVSENVASRIEVPDSFYSLSADEIKREADLRRKKIAESQLLIPRSYKEKQAKAARKRYKRSMIRVQFPDGVVLQGVFAPWEPTFALYEFVSSALKEPSLQFELLDPVLVKRRVIPHTPAPGQKPITLEDEELVPSALIRFRPIETDSLVFTGLRNELLEISEPLS
The following text describes a protein:
Function:
Plays two roles in the biosynthesis of the exopolysaccharide alginate: protects alginate from degradation as the polymer traverses the periplasm, and also plays a role in its O-acetylation. Acetylation of alginate causes the cells in the biofilm to adhere better to lung epithelium, form microcolonies, and resist the effects of the host immune system and/or antibiotics. Displays a low acetylesterase activity in vitro using a pseudosubstrate, 3-carboxyumbelliferyl acetate. Probably has acetyltransferase activity in vivo.
Subcellular Localization:
Periplasm.
Domain:
Consists of two domains, with an N-terminal domain with structural homology to members of the SGNH (GDSL) hydrolase superfamily and a C-terminal carbohydrate-binding module (CBM) that may bind alginate.
Sequence:
MKTRTSRLFRLSALAAGLCLAQAALAADPGAAPSYQALPAGNLCPAAAYDSRYNTKYLGFFTHLVQAQDDWLFRTTYDLRTDFGTSAEGWRELRALRDELKRKGIELVVVYQPTRGLVNREKLSPAEKAGFDYELAKKNYLATIARFRQAGIWTPDFSPLFDEKEEHAYYFKGDHHWTPHGARRSAKIVAETLKQVPGFEEIPKKQFESKRVGLLSKLGTFHKAAAQLCGNSYATQYVDRFETEPVGASDSGDLFGDGGNPQIALVGTSNSGPAYNFAGFLEEFSGADILNNAVSGGGFDSSLLAYMTSEEFHKNPPKILIWEFATHYDMAQKSFYRQAMPLVDNGCSGRKTVLSRKVKLRQGRNEVLLNSAALPIRSGSYVADVTYSDPSVHELKNTIWYMNGRREQLKIEQSKAVDTGGRYVFQLRNDSDWADQQFLSLEIEAPEDMPQGLEVQASICQAAPAKASQSVAGR
The following text describes a protein:
Function:
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of cyclin-D1 (CCND1) and its subsequent proteasomal degradation. However, it does not act as a major regulator of CCND1 stability during the G1/S transition. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. Promotes ubiquitination of FXR1 following phosphorylation of FXR1 by GSK3B, leading to FXR1 degradation by the proteasome.
Subcellular Localization:
Cytoplasm.
Sequence:
MAGSEAGSRGSSPQPHHSDWGRLEAAILSGWRSFWQSVGKERAAPRASPEEADEEGNPLSRLPIDVQLYILSFLSPHDLCQLGSTSHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPIFEVTAGSFFDYMAVYKMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVSFQLHNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSLQNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSRIMAMTDPAFGSSGRPMLVLSCISQANVKRMPCFYLAHELHLNHLNHPWMVQDTEAETLTGFSNGIQWILEEVESKHAR
The following text describes a protein:
Function:
Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells. Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Plays a role in clathrin-mediated endocytosis. Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity. Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells.
Subcellular Localization:
Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle.
Domain:
The FXDXF motif mediates interaction the AP-2 complex; The clathrin box motif mediates interaction with clathrin.
Sequence:
MDCRTKANPDRTFDLVLKVKCHASENEDPVVLWKFPEDFGDQEILQSVPKFCFPFDVERVSQNQVGQHFTFVLTDIESKQRFGFCRLTSGGTICLCILSYLPWFEVYYKLLNTLADYLAKELENDLNETLRSLYNHPVPKANTPVNLSVNQEIFIACEQVLKDQPALVPHSYFIAPDVTGLPTIPESRNLTEYFVAVDVNNMLQLYASMLHERRIVIISSKLSTLTACIHGSAALLYPMYWQHIYIPVLPPHLLDYCCAPMPYLIGIHSSLIERVKNKSLEDVVMLNVDTNTLESPFSDLNNLPSDVVSALKNKLKKQSTATGDGVARAFLRAQAALFGSYRDALRYKPGEPITFCEESFVKHRSSVMKQFLETAINLQLFKQFIDGRLAKLNAGRGFSDVFEEEITSGGFCGGNPRSYQQWVHTVKKGGALFNTAMTKATPAVRTAYKFAKNHAKLGLKEVKSKLKHKENEEDYGTCSSSVQYTPVYKLHNEKGGNSEKRKLAQARLKRPLKSLDGALYDDEDDDDIERASKLSSEDGEEASAYLYESDDSVETRVKTPYSGEMDLLGEILDTLSTHSSDQGKLAAAKSLDFFRSMDDIDYKPTNKSNAPSENNLAFLCGGSGDQAEWNLGQDDSALHGKHLPPSPRKRVSSSGLTDSLFILKEENSNKHLGADNVSDPTSGLDFQLTSPEVSQTDKGKTEKRETLSQISDDLLIPGLGRHSSTFVPWEKEGKEAKETSEDIGLLHEVVSLCHMTSDFQQSLNISDKNTNGNQT
The following text describes a protein:
Function:
Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
Subcellular Localization:
Cytoplasm.
Sequence:
MVRIFLTGYMGAGKTTLGKAFARKLNVPFIDLDWYIEERFHKTVGELFTERGEAGFRELERNMLHEVAEFENVVISTGGGAPCFYDNMEFMNRTGKTVFLNVHPDVLFRRLRIAKQQRPILQGKEDDELMDFIIQALEKRAPFYTQAQYIFNADELEDRWQIESSVQRLQELLEL
The following text describes a protein:
Function:
Part of the tripartite ATP-independent periplasmic (TRAP) transport system.
Subcellular Localization:
Cell inner membrane; Multi-pass membrane protein.
Sequence:
MIGIVMFFVALFALLLGFPVAFTFGGIALIFGLWAEGIEMFAFMPYRIQSIMENTVLMAVPLFVFMGLVLQKTKLAEQLLESMGRLFGGIHGGVAISTVLVGTLLAASTGVVGASVVAMGLISLPVMLKYRYDKGLACGTICASGTLGQIIPPSIVLILLGDVLGVPVGDLFQAALWPGLMLVAAYIIYILIYAKLNPQAAMPIEPDADLSRRQEVVKALKAVIPPLALIVVVLGSIFAGVATPTESAALGGAGAILLALAYGQFSWSMVYDASKETVKVTAMVFAILLGATAFSMAFTYTGGDELVEEWMMNLPGDKWGFLIVTMLVILILGFFIDFVEICFIIVPILAPVAVLMDVDMTWFAILIAMNLQTSFLTPPFGFSLFYLKGVAPKSVTTMDIYRGVVPFILIQILVLISLMVFPSFYGM
The following text describes a protein:
Function:
Cell surface proteoglycan.
Subcellular Localization:
Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
Sequence:
MAPAGEFSSLVLLAGLMALATISTAASAKGQLSCKEVRASFHSLQPGVRWVPESPVSGTDLQVCVTKTSTCCNRRMEDRYQIVSRLNMEQLLQSASTKLKFLIIQNAAIFQEAFEMVIRHARNYTNTMFKNHYQNISAKAMKFVGELFTDISLYLLGSDINVNDMVNEFFDSLFPVLYSHYLNPGVHETVENVECLRLARSDTNAFGQYPKTIMTRVSKSLQASRAFLQALNLGIEVINTTDYLKINKDCGRTLMKMWYCSHCQGVLEAKPCAGYCGSTMRACLAGVVDINIHWEEYILSIERHTREMYGIYDLENVLLNLFSLIREAVLHVEKNWGKLNTSINKLCSPSKQRISRSVHNTGEFFINKKEVKTHTDHEETLSGKRREFVAKLKTYKHFYGDLPAEVCQHTAVVENDTFCWNGHEIVERFSPHTVKNGLKIHSSHQEGKIRGSEPVINQIIDKLNHINQLLKGMSSQRQKYLGKFTEEEEAESGDCDDEDDCDGGSGKNSLRIRNKLRFIPEMDYDFDIEDIPFNKQVRNQQEHNGREITHLQSGSDYKETVLARLFPLFIAAIFGILIMV
The following text describes a protein:
Function:
Mediates H(+)-dependent pyridoxine transport.
Subcellular Localization:
Lysosome membrane; Multi-pass membrane protein.
Sequence:
MGAGPSAEGEREPLLDQVQSRVYGKRWLVLAIFSALGFMQGMVWNTWGPIQISAKQVYGFTGIDIAMLVLWGSIGFIPWMLFMWVMDKRGLRMSLLISSSLMLLGTAVRSIPLHDLSVKKWLIHGGQLLNGLAGPTIMSAPPLLSTTWFAPDQRATATAVASLVSYLGSAGSFLIGPLVVPAPNETQPLRSVIGRTNDHIRDRIQIVLYAEFAMTAVLFSAVVLYFPSRPPLPPSVAAASQRLGYRSSICRLLSNVRFLMIALAYSVPTGVVSGWSGVLDMVLTPAKVSQVDAGWIGFWSIVGGCVVGVAMARFADSIRGMLKLILLLMLAGATLASVWFTLTCLTSITHLPSTAATLYTSCILVGIFINSSVPIFFELLMETVYPVPEGITCGLVTFLTNLFMGILMFILTVYSKDLSWLNWCLTGSCVFSLLLILFFRESYDRLYLDVFVSV
The following text describes a protein:
Function:
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Miscellaneous:
MISCELLANEOUS: IL1B production occurs in 2 steps, each being controlled by different stimuli. First, inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1B (priming). Then additional signals are required for inflammasome assembly, leading to CASP1 activation, pro-IL1B processing and eventually secretion of the active cytokine. IL1B processing and secretion are temporarily associated.
Subcellular Localization:
Cytoplasm, cytosol. Secreted. Lysosome. Secreted, extracellular exosome. Note=The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10.
Sequence:
MPAYGEQDSSREVEETDDKKTKRQPGLESTMARVPDQSRDLMECYSGDGEDQFYEVDGPSQIKSGFQDLKARTCQETRIHKEDKCSPCQMGIELKVTELPSSHGFRKAVVLVVAVERIKRQAVSYNTSFMDRDLMDIFTSIFKEEPISCSTWEQTLVTDSLYHYLRCQEVTIWDEEHKSFTLNTMANPCELRALHLIGANATQEVKLNMNFYYKTERLAGPTVKQPVTLGIKGGNPGNLYLSCVKKGGKPTLQLEVVNKSDLLGKNQERFIFNKSTEGTSTTFESAAYPDWYISTSREEDEPVFLGASKGEEAITNFFLH
The following text describes a protein:
Function:
Receptor for gastrin and cholecystokinin. The CCK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular Localization:
Cell membrane; Multi-pass membrane protein.
Sequence:
MDPRRLNESLQELLCRPGNGTGSANGTGTGPTWNGSACDLLRRGLRGPQAPKELDLTVRVLLYALIFVLSVCGNALVVAVLLLNRRLRTVTNSFLLSLALSDLMLALCCMPFTLIPNLMGTFVFGEAVCKLMAYLMGVSVSVSTFSLVAIAIERYSAICNPLQSRVWQTRSHACRVIASTWLFSALLMLPYAVYSTTRLLPVPGARPPLSQCTHAWPSEHVRQAWYVLLLLVLFFIPGVVMIVAYGLISRELYRGIRFELDIKGEAAVVCPVPFPVPFPVPCPAPPESSWCQIHDHATPACSPAQHRTVAHLRRGGRLLPAALTAGRCAGAAGAGHGGGTTGAGAHQQLRGKAGGQAARHPHAGGHRGHVLPLLAAHLCGQHVACLRTAGSPARALGDAHLLHPPAVLHLRLRQPAHLLLHEPPLPQRLPGHLCRLPPCLPAPRARRGRGQRQRIALQVQLHHCQQPRPPLSPPAPSPPPDGSSLAPCHGPVVVPRIPASCAPRAPRAAPHTPVPAGSLLRCSCVGGRGDAGRAPEA
The following text describes a protein:
Function:
Functions as transport protein in the blood stream.
Subcellular Localization:
Secreted.
Sequence:
MALSWVLTVLSLLPLLEAQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQNQCFYNSSYLNVQRENGTVSRYEGGREHAAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES
The following text describes a protein:
Function:
Acyltransferase involved in the biosynthesis of membrane phospholipids. It introduces a cis-vaccenoyl group (18:1) into the sn-2 position of phospholipids. Overproduction of YihG facilitates the synthesis of phospholipids containing a myristoyl group (14:0) and a cis-vaccenoyl group at the sn-2 position, and a cis-vaccenoyl group at the sn-1 position. May affect flagellar formation through modulation of the fatty acyl composition of membrane phospholipids.
Miscellaneous:
MISCELLANEOUS: Overexpression of the gene in E.coli carrying a temperature-sensitive mutation in plsC allows its growth at non-permissive temperatures.
Subcellular Localization:
Cell inner membrane; Single-pass membrane protein.
Domain:
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Sequence:
MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVKLLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICNHRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRHPERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIAMALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYINDKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK
The following text describes a protein:
Function:
Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerisation to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules. Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle. The DASH complex mediates bipolar kinetochore-microtubule attachments and facilitates the formation of additional interactions between outer kinetochore components and spindle microtubules. During chromosome movement along the microtubule, it is required both for the sliding of kinetochores along the lateral side of the microtubule and also for microtubule end-on pulling on the kinetochore. Modulates cytoplasmic microtubule dynamics by tracking the plus-end of shortening microtubules and slowing their depolymerization.
Subcellular Localization:
Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton. Note=Associates with the mitotic spindle and the kinetochore (By similarity). In the cytoskeleton, localizes to cortical microtubules (Probable).
Sequence:
MSLEEKRALQNQILAEYANLASNLETLVKVLQDMVYNPSNNILDSLRDLEKEVGLVYTLYKASVWAILADLENNQEGKSGMFQDES
The following text describes a protein:
Function:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).
Subcellular Localization:
Nucleus.
Sequence:
MSTGEAQQLIQKLLLEALARRLAQPKWLSLLHQLVRRIEAAQDDDGTFDVIPSLEGSHAAECLVKYLLSQSFIDPLLIEYLQALIYGSANRSGVSPDQGPITDVMTVTLHLLANVQAAASNVPAIEIISSVIGQGLLMTFQAPVPFRVSSPSFLALLQRLFADPTYEQDQTPSFPVDHVDTGASDIKVAPASLGFVVANLRLLALAADPAIASPQNLLAPRVSITVLINVGQGLLAYVTQILSAAQTAKNDKLKPDGAILQQLRSMASTSLTEIEAVLRSMNPAWKDEIALLRSLTSQIGVIDQISESFAEANTSKLSTRPTKRKLETLQDAQQRATWDHFLDVSTIAASEKPAVEPETALLMHLLVDHHTSWDFKLDAIKTLFLARRSAAAPSLTLEKSLTAFYFELLVAAIDACAAVVEMPPAFKGAEVYATIWRNALCGMIPELVLQLEQWLDGNQELPLRGQRLEAPHVRLEAALRAALLVMSDRLNVCESATNQSHAHGNVAATNGEEAAGAHANMMSDVLGLGTPPSQPIRAWLLRACIEHSLARPEAIADEFANGHKLASEVQSLTQSLRMDAQLEGLALNTLFETRIPTDHPVELLQRVASDPGTHFIFARQLVLQVQGWLEQHDLESIARWCKALTENACEGGAMLDTIMIYIDPAQMLDPLASILDHQDLGQTSDEPSTLSDILLFVQLLCYRYSTPITRISRYTVSNHDMDTVDSSAPLHRSMPPFLATHLATSSVSYPLSVLSEEDRGLVSRWIHALFGNEGISDDLISASPPTTLLRLSPLLFSQSISACQHGIIDLETLRGGLSYFLQDLLSFALPGALVWLLSEISRTPLQPILDFLSESGLQASVVDVPSSDGTLANGLPRNATSKSVCLEVLALLVDTDACPSSVRQLIAKAFDAFVSTIEADVTAAPQLAVGCETFNLASLKARMEGAGVTARLLSETGASWLQSVAAGHQDSRSKLFSMLTTLQPSVHAMDRLIGALKSGQVGRNGRDQKGLAAWLCFIAPASEPTSALRFVQRLELGDIEESAIEGVVRIVGLVVGLIMAAEAQRATTDAVADDLLALARAADTNARGTKATVADTTAAARPAEADLFDDEPCSPQPPHAAANATSHTSNAAPTVTSAASLLASGELRTTSKQVLDMMALKLVRFRSHMLGEKNAKSTTISAKWATLQSAIADHLAGGEAWHGAQQCRERTQNDAPVNGGKDADSGSGLSAWLSAMS
The following text describes a protein:
Function:
[Isoform 2]: Transports organic anions in a voltage-driven, multispecific, manner, on the apical side of renal proximal tubule. In particular, participates in the secretion of urate from the cell into the lumen. Urate is the end product of purine metabolism. May have roles in the metabolism and secretion of estrone sulfate, estradiol-17-beta-glucuronide, ochratoxin A, as wells as drugs such as bumetanide.
Subcellular Localization:
[Isoform 1]: Endoplasmic reticulum membrane; Multi-pass membrane protein; [Isoform 2]: Cell membrane; Multi-pass membrane protein.
Sequence:
MATKTELSPTARESKNAQDMQVDETLIPRKVPSLCSARYGIALVLHFCNFTTIAQNVIMNITMVAMVNSTSPQSQLNDSSEVLPVDSFGGLSKAPKSLPAKSSILGGQFAIWEKWGPPQERSRLCSIALSGMLLGCFTAILIGGFISETLGWPFVFYIFGGVGCVCCLLWFVVIYDDPVSYPWISTSEKEYIISSLKQQVGSSKQPLPIKAMLRSLPIWSICLGCFSHQWLVSTMVVYIPTYISSVYHVNIRDNGLLSALPFIVAWVIGMVGGYLADFLLTKKFRLITVRKIATILGSLPSSALIVSLPYLNSGYITATALLTLSCGLSTLCQSGIYINVLDIAPRYSSFLMGASRGFSSIAPVIVPTVSGFLLSQDPEFGWRNVFFLLFAVNLLGLLFYLIFGEADVQEWAKERKLTRL
The following text describes a protein:
Function:
Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.
Miscellaneous:
MISCELLANEOUS: Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.
Subcellular Localization:
Cell inner membrane; Multi-pass membrane protein.
Sequence:
MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLVGVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPGVSLVYATLLGIAGFMLLWFGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGDFDSGAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAVATLMLTLGGYAGYKYLVVAAAVSVWWLGMALRGYKVEDDKVWARKLFGFSIIAITALSIMMSVDFMVPNSQNLLTYVW
The following text describes a protein:
Function:
Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
Subcellular Localization:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.
Sequence:
MAGSALRKGYLVLYNAASAVAWATILGRVASVYFAKGAPFVPLVVDDFARVTQTFAVMEIFHALTGIVPAPIFTTVMQVASRLMLVWGISYPFPHLNSSAWYTSMLTAWSTTEVIRYSYFALKQVDFIPYWLHWLRYSAFLVLYPMGISSEVAMIFKALVGPADQLATWYPYALVAVLLSYIPGSVVLYSHMLSQRRKQVGGGAIKEKKEKKRQ