The following text describes a protein:
Function:
[Capsid protein VP0]: Forms an icosahedral capsid of pseudo T=3 symmetry together with capsid proteins VP1 and VP3 (By similarity). The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity). Capsid proteins interact with host alpha-V/beta-3 integrin heterodimer to provide virion attachment target cell (By similarity). This attachment induces virion internalization predominantly through clathrin-mediated endocytosis (By similarity). Binds packaging signals present in the viral RNA (By similarity); [Capsid protein VP3]: Forms an icosahedral capsid of pseudo T=3 symmetry together with capsid proteins VP0 and VP1 (By similarity). The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity). Capsid proteins interact with host alpha-V/beta-3 integrin heterodimer to provide virion attachment target cell (By similarity). This attachment induces virion internalization predominantly through clathrin-mediated endocytosis (By similarity). Binds packaging signals present in the viral RNA (By similarity); [Capsid protein VP1]: Forms an icosahedral capsid of pseudo T=3 symmetry together with capsid proteins VP0 and VP3 (By similarity). The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity). Capsid proteins interact with host alpha-V/beta-3 integrin heterodimer to provide virion attachment target cell (By similarity). This attachment induces virion internalization predominantly through clathrin-mediated endocytosis (By similarity). Binds packaging signals present in the viral RNA (By similarity); [Protein 2A H-NC]: Is not a protease; [Protein 2B]: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host endoplasmic reticulum and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cytoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication; [Protein 2C]: Induces and associates with structural rearrangements of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3; [Protein 3A]: Localizes the viral replication complex to the surface of membranous vesicles (By similarity). It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the disassembly of the Golgi complex, possibly through GBF1 interaction (By similarity). This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface (By similarity). Plays an essential role in viral RNA replication by recruiting ACBD3 and PI4KB at the viral replication sites, thereby allowing the formation of the rearranged membranous structures where viral replication takes place (By similarity); [Viral protein genome-linked]: Acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. Following genome release from the infecting virion in the cytoplasm, the VPg-RNA linkage is probably removed by host TDP2. During the late stage of the replication cycle, host TDP2 is excluded from sites of viral RNA synthesis and encapsidation, allowing for the generation of progeny virions; [Protease 3C]: Cysteine protease that generates mature viral proteins from the precursor polyprotein (By similarity). In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease (By similarity); [RNA-directed RNA polymerase 3D-POL]: Replicates the viral genomic RNA on the surface of intracellular membranes. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated.
Subcellular Localization:
[Capsid protein VP3]: Virion. Host cytoplasm; [Capsid protein VP1]: Virion. Host cytoplasm; [Protein 2A H-NC]: Host cytoplasm. Host nucleus, host nucleolus; [Protein 2B]: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum; [Protein 2C]: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum; [Protein 3A]: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum; [Viral protein genome-linked]: Virion; [Protease 3C]: Host cytoplasm; [RNA-directed RNA polymerase 3D-POL]: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).
Domain:
[Capsid protein VP0]: The N-terminus mediates the interactions among pentamers (By similarity). In order to facilitate delivery of the virus genome into the cytoplasm, the N-termini of VP0s have to be released from contacts between pentamers and exposed at the particle surface, resulting in capsid disruption (By similarity); [Capsid protein VP3]: The N-terminus binds RNA; [Protein 2A H-NC]: Contains a H-NC box.
Sequence:
METIKSIADMATGFTNTIDSTVNAVTEGVSKIGNDSGGEILTKVADDASNLLGPNCVASTSQPENKDVVQATTTVNTLTNLTQHPSAPTMPFTPDFSNVDVFHSMAYDITTGDKNPSKLIRLDTTTWQHTWPRQHLINDVELPKAFWDKNSKPAYGQSRYFAAVRCGFHFQVQINVNQGTAGCALVVYEPKPIVTHGGHLEFGSYTNLPHVLMNLAETTQADLCIPYVSDTNYVKTDSSDLGRLRVYVWTPLTIPSSATNDVDVTVLGSLLQLDFQNPRTYDTDVNIYDNSPLDTKTKYGKLRFSKKILSMSTKYKWTRNKIDIAEGPGSMNMANVLSTTGAQSIALVGERAFYDPRTAGSKSRFGDMIHIAQLFSVMSDTTTPSTSSGIDDLGYLDWSATYVPQQVIHRNVVKLNQFSNLKPFVNAYTYFRGSLVLRMSVYASTFNRGRLRMGFFPNFTTNTTSEMDNAIYTICDIGSDNSFEITIPYTFSTWMRKTNGRPIGLFQVEVLNRLTYNSSCPNKVHCIVQGRLGNDARFYCPTGSLVEFQNSWGSQMDLTDPLCVEDDEAEDCKQTISPDELGLTSAQDDGPLGVEKPNYFLNFRAINVDIFTVSHTKVDNIFGRAWLALEHTFADDGTWRADLNFPTQGHGTLTRLFTYYSGELNVHVLYLSDNGFLRVTHAYDHDNDRSNFLSSNGVITVPAGEQMTLSVPFYSSKPLRTIRETGALGKLICKPLLSGTHSGKIEVYLSLRCPNLFFPSPAPKEKTSRALRGDLANFIDQSPYGQQQQTQMMKLAYLDRGFYKHYGIIVGGYVYQLDSDDIFKTALTGKARFTKTRLTPDWIVEEECELDYFRVKYLESSVNSEHIFSVDSNCETIAKDIFGTHTLSQHQAIGLVGAILLTAGLMSTIKTPVNATTIKEFFNHAIDGDEQGLSLLVQKCTTFFSSAATEILDNDLVKFIVKILVRILCYMVLYCHKPNILTTACLSTLLIMDVTSSSVLSPSCKALMQCLMDGDVKKLAEVVAESMSNTDDDEIKEQICDTVKYTKTILSNQGPFKGFNEVSTAFRHVDWWIHTLLKIKDMVLSVFKPSIESKAIQWLERNKEHVCSILDYASDIIVESKDQTKMKTQEFYQRYSDCLAKFKPIMAICFRSCHNSISNTVYRLFQELARIPNRISTQNDLIRVEPIGVWIQGEPGQGKSFLTHTLSRQLQKSCKLNGVYTNPTASEFMDGYDNQDIHLIDDLGQTRKEKDIEMLCNCISSVPFIVPMAHLEEKGKFYTSKLVIATTNKSDFSSTVLQDSGALKRRFPYIMHIRAAKAYSKSGKLNVSQAMSTMSTGECWEVSKNGRDWETLKLKDLVQKITEDYQERQKNYNAWKQQLENQTLDDLDDAVSYIKHNFPDAIPYIDEYLNIEMSTLIEQMEAFIEPRPSVFKCFAVKLPHKPGKQPRKLWAGSAGKIKSMLSFIERNKAWLTVVSAVTSAISILLLVTKIFKKEESKDERAYNPTLPITKPKGTFPVSQREFKNEAPYDGQLEHIISQMAYITGSTTGHLTHCAGYQHDEIILHGHSIKYLEQEEDLTLHYKNKVFPIENPSVTQVTLGGKPMDLAILKCKLPFRFKKNSKYYTNKIGTESMLIWMTEQGIITKEVQRVHHSGGIKTREGTESTKTISYTVKSCKGMCGGLLISKVEGNFKILGMHIAGNGEMGVAIPFNFLKNDMSDQGIITEVTPIQPMYINTKSQIHKSPVYGAVEVKMGPAVLSKSDTRLEEPVDCLIKKSASKYRVNKFQVNNELWQGVKACVKSKFREIFGVNGIVDMKTAILGTSHVNSMDLSTSAGYSLVKSGYKKKDLICLEPFSVSPMLEKLVQDKFHNLLKGNQITTIFNTCLKDELRKLDKIAAGKTRCIEACEVDYCIVYRMIMMEIYDKIYQTPCYYSGLAVGINPYKDWHFMINALNDYNYEMDYSQYDGSLSSMLLWEAVEVLAYCHDSPDLVMQLHKPVIDSDHVVFNERWLIHGGMPSGSPCTTVLNSLCNLMMCIYTTNLISPGVDCLPIVYGDDVILSLDREIEPERLQSIMADSFGAEVTGSRKDEPPSLKPRMEVEFLKRKPGYFPESTFIVGKLDTENMIQHLMWMKNFSTFKQQLQSYLMELCLHGKDIYQRYIKILDPYLKEWNIVVDDYDVVIAKLMPMVFD