The following text describes a protein:
Function:
RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated.
Miscellaneous:
MISCELLANEOUS: Classified as His(+) endonuclease since it has a histidine upstream of the active site that coordinates the first cation.
Subcellular Localization:
Virion. Host cytoplasm.
Domain:
The N-terminus contains the endonuclease activity (endoN). The central region contains the RdRp activity.
Sequence:
MNIQKIQKLIENGTTLLLSIEDCVGSNHDLALDLHKRNSDEIPEDVIINNNAKNYETMRELIVKITADGEGLNKGMATVDVKKLSEMVSLFEQKYLETELARHDIFGELISRHLRIKPKQRNEVEIEHALREYLDELNKKSCINKLSDDEFERINKEYVATNATPDNYVIYKESKNSELCLIIYDWKISVDARTETKQWRNTYKNIWKSFKDIKVNGKPFLEEHPVFVSIVILKPIAGMPITVTSSRVLEKFEDSPSALHGERIKHAKNAKLLNISYVGQIVGTTPTVVRNYYANTQRIKSEVRGILGDDFGSKDVFFSHWTSKYKERNPTEIAYSEDIERIIDSLVTDEIPREEIIHFLFGNFCFHIETMNDQHIADKFKGYQNSCINLKIEPKADLADLKDHLIQKQQIWESLYGKHLEKIMLRIREKKRKEKEIPDITTAFNQNAAEYEERYPNCFNDLSELKLTFHDLVPSLKIELSSEVDYNNAIINKFRESFKSSSRVIYNSPYSSINNQTNKARDITNLVRLCLAELSCDTTKMEKQELEDEIDINTGSIKVERTKKSKEWNKQGSCLTRNKNEFCMKDTGRENKTTYFKGLAVMNIGMSSKKRILKKEEIKERISKGLEYDTSERQADPNDDYSSIDMSSLTHMKKLIRHDNDDSLSGKRFKGSFFLLHNFNIIEDGKITSVFNNYAKNPECLYIQDSVLKTELETCKKINKLCNDLAIYHYSEDMMQFSKGLMVADRYMTKESFKILTTANTSMMLLAFKGDGMNTGGSGVPYIALHIVDEDMSDHFNICYTKEIYSYFRSGSNYIYIMRPQRLNQVRLLRLFKTPSKVPVCFPQFSKKANEIGKSLKNKDIEKVNLFSMTMTVKQILINIVFSSVMIGTVTKLSRMGIFDFMRYAGFLRLSDYSNIKEYIRDKSDPDITNCGRYLFRNGIKKLLFRMEDLNLSTNAKPVVVDHENDIIGGITNLNIKCPITGSTLLTLEDLYNNVYLAIYMMPKSLHNHVHNLTSLLNLPAEWELKFRKELGFNIFEDIYPKKAMFDDKDLFSINGALNVKALSDYYLGNIENVGLMRSEIENKEDFLSPCYKISTLKSSKKCSQSNIISTDEIIECLQDAKIQDIENWKGNNLAIIKGLIRTYNEEKNRLVEFFEDNCVNSLYLIEKLKEIISSGSITVGKSVTSKFIRNNHPLTVETYLKTKLYYRNNVTVLKSKKVSEELYDLVKQFHDMMEIDLDSVMNLGKGTEGKKLTFLQMLEFVMSKAKNVTGSVDFLVSVFEKMQRTKTDREIYLMSMKVKMMLYFIEHTFKHVAQSDPSEAISISGDNKIRALSTLSLDTITSYNDILNKNSKKSRLAFLSADQSKWSASGLTTYKYVLAIILNPILTTGEASLMIECILMYVKLKKVCIPTDIFLNLRKAQQTFGENETAIGLLTKGLTTNTYPVSMNWLQGNLNYLSSVYHSCAMKAYHNTLECYKNCDFQTRWIVHSDDNATSLIASGEVDKMLTDFSSSSLPEMLFRSIEAHFKSFCITLNPKKSYASSSEVEFISERISKWSDYSSLLQAFSKLLHRIFAYKLFDDLMSLSIHVTMLLRKGCPNEVIPFAYGAVQVQALSIYSMLPGEVNDSIRIFNKLGVSLKSNEIPTNMGGWLTSPIEPLSILGPSSNDQIIYYNVIRDFLNKKSLEEVKDSVSSSSYLQMRFRELKEKYERGTLEEKDKKMIFLINLFEKASVSEDSDVLTIGMKFQTMLTQIIKLPNFINENALNKMSSYKDFSKLYPNLKKNEDLYKSTKNLKIDEDAVLEEDELYKKIASSLEMESVHDIMIKNPETILIAPLNDRDFLLSQLFMYTSPSKRNQLSNQSTEKLALDRVLRSKARTFVNISSTVKMTYEENMEKKILEMLKFDLDSYCSFKTCVNLVIKDVNFSMLIPILDSAYPCESRKRDNYNFRWFQTERWIPVVEGSPGLVVMHAVYGSNYIENLGLKNIPLTDDSINVLTSTFGTGLIMEDVKSLVNGKDSFETEAFSNSNECQRLVKACNYMIAAQNRLLAINTCFTRKSFPFYSKFNLGRGFISNTLALLSTIYSKEESYHFVSTASYKLDKTIRTVVSAQQDMNLEKILDTAVYISDKLQSLFPTITREDIVLILQNVCLDSKPIWQSLEDKMKKINNSTASGFTVSNVILSHNSELNTIQKQIVWMWNMGLCSHRTLDFVIRYIRRRDVRYVKTEEQDESGNYVSGTMYKIGIMTRSCYVELIASDQDVAVSLRTPFEILNEREYLFDTYRESIEKLLAEIMFDKVNIINQTTTDCFLRTRRSCIRMTTDNKMIVKVNATSRQIRLENVKLVVKIKYENVNSDVWDIIESQKSLVLRLPEVGEFFSDMYKTADSETETIKTIKNRLMTSLTFIEAFGNLSQQIKEIVDDDIRETMDEFLMNIRDTCLEGLENCKSVEEYDSYLDENGFNDTVELFENLLRTHDNFENEYSPLFSEIVDKAKQYTRDLEGFKEILLMLKYSLINDASGFKSYRATGMHAVELMAKKHIEIGEFNLLGMIQLIKACETCHNNDSILNLASLRNVLSRTYATFGRRIRLDHDLDLQNNLMEKSYDFKTLVLPEIKLSELSREILKENGFVISGENLKMDRSDEEFVGLASFNVLRLDEEEMYEGLIKEMKIKRKKKGFLFPANTLLLSELIKFLIGGIKGTSFDIETLLRNSFRPDIFSTDRLGRLSSSVPALKVYATVYMEYKNVNCPLNEIADSLEGYLKLTKSRSKEHFLSGRVKKALIQLRDEQSRTKKLEVYKDIANFLARHPLCLSEKTLYGRYTYSDINDYIMQTREIILSKISELDEVVETDEDNFLLSYLRGEEDAFDEDELDEEEDTD