The following text describes a protein:
Function:
Ribosome collision sensor that activates a translation quality control pathway when a ribosome has stalled during translation (By similarity). Directly binds to the ribosome and acts as a sentinel for colliding ribosomes (By similarity). GCN1 also acts as a positive activator of the integrated stress response (ISR) by mediating activation of GCN2 in response to low amino acid, carbon, or purine availability. Component of the GCN1-GCN20 complex that forms a complex with GCN2 on translating ribosomes: during this process, GCN1 acts as a chaperone to facilitate delivery of uncharged tRNAs that enter the A-site of ribosomes to the tRNA-binding domain of GCN2, and hence stimulating GCN2 kinase activity, leading to phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2). eIF-2-alpha/SUI2 phosphorylation converts eIF-2-alpha/SUI2 into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator GCN4, and hence allowing GCN4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (By similarity).
Subcellular Localization:
Cytoplasm.
Domain:
The EF3-like region is necessary and sufficient for interaction with GCN20; The RWDBD (RWD-binding domain) region mediates binding to RWD domain-containing proteins, such as GCN2.
Sequence:
MSVEEPGIEAHGHKDRMLYAMLLSKDTSLAFLGSKKIMIDILQHICRTQDIDEESAIAALEDIFETLPRNLSRDARKQIEITINHLVSRLPSIVLPFLVRRLTTIAGRLDRFRSTVSVSFDCLNWVNSMIPNLPEKELQYWILELLPLQSSFLSYALRDGKPSVADSAIKSTRRCYRSLFCKKMDSLKKLVSFLLTETENAILPPKSLPLYGVIISTCYYFHQSPNPRNEISQQAELFSKIMAQNVLMAKPALEKYLYHEFCYSLGLLLSVDQLKLYLLPSIEKALLRSPEIIFSGILSSLAHGFADSKVDASSLILSSVLTSFVNGLKSSNAEVRRNCFQTFKDLSANASDNESLSRVASELITSLRTGKVTASDQRVLFVDALSSLSLKHIDASMLLNELLPLFTKAKESDFNSLASLIVKTLKFLLMNGRNPGDKIYDFLSKSLQRPVAHESMFWLTSLATMAWDLPASDDVQIEFINFFLNNLSILTEKALMSVSGATQNGTYLAPIIYLSFGVNKLSVWNSERISHTLELQDILVKLSTPKNNDVFIFSSKITNKLNDDQSKLWYFQGLCDFAKVSDNLLFSNFVERWFQSVIGVFSFASRENSNRALKILKSAILYRPHLRMSICSQLWNYHADFEKSKSVGKFDSAKYDEISSLFQSLILSSMSADTSNFSNQELVDFDKYLVELLFLSFAFKDKFDWIRFCQVSKRDPATLVSERIHSIIEEIELLLSSAIKDSKETAAIASISMIVFVAPEESIPLFVNVFRNQLLHLNISSVSSTDLEIWKTPEGVLWDNVLEKKSSKKLDKNTKDYETKRWEAEVRAKQSAKKPAKLSKDQQALVDAQLDAEAKIRSRVNLIALSLERGLGIIRSLGEAVQLAPALWVEDAIDVLLFHNVLKYSEPFLKNLAYDTFLLTLKASGFSERLGDRSYSSSLASILAHTFSVNSSENIKELTKSILYKLRFAIEQNYFEPQMFACIFPLLYDLTFNITNSDEEDEAELQLLVTEILEFQALYSASLRRMRSKLIKSLLHLLEIAPTQYQENKNSLLSLCEGLHSTYTDEELNLLLSNLFHPESSIRSAVLQALQAFDLSRFEFIKEIFLELYDDNETNASIAHQISTQNGLDATETSFFELQIFFTQDSDYLQQIIGKSLIDLLDEFEELGQFIPKELMRTYRENALPSAPEYDEYGIIKKETIGRDLGRIARESVAVSFFHISKYLSSNLLLPFLEFLLTASEAEAQIPVTDASQKVSSKMLEAGKLAIFQSGAHQVEALMELFEQKLNVDSLPTDANDRLREATVVLFGTVAQHLPSNDPRLAVVMDSLLSVLSTPSESVQLAVAVCLPPLVKKSLGKSKEYYELLSNKLMNSTSLADQKGAAYGLAGLVKGYGIKAFQDFNILDSLSELISNRQNATHRQVALFAVEAFSRILGIYFEPYLPDLLPLLLTSFGDNANEVREATMDAVKQIMSQLSAFGVKLLLPTLLDGLNEYNWRSKKASVEILGLMSYMAPKQLSVFLPTIIPKLSEVLTDSHSQVRNTANKSLLRFGDVISNPEIQTLVPTLLKALSDCTRYTDDALEALLKTSFVHYLDPPSLALVIPILKYGLRERNAGTKRQSAKIFGLMASLTEPENLAVYLESLMPRLREVLIDPVPDTRATAAKALGSLIEKLGEKKFPTLIPELFNVLRSECSEVDRQGAAQGLSEILAGLGLARLEDVLPEILKNTSSPVPHIRESFISLLIYLPATFGSRFQPYLARAIPPILSGLADDSELVQTASLRAAKMIVNNYATKSVDLLLPELEKGLFDNAWRIRLSSVQLVGDLVFKLAGINRKALQEDEEEEGTHSDVSRKALLDIIGQERHDRILSTLYIVRQDIAAVVRTPAIQIWKAIVVNTPRTVREILPTLTSIIVSNLNSSSNDRRTMCVKSLGDLLKKAGFDVLPQLLPVLKQGLESANSGDRIGVCIALEELINSATPEQLEIYSDDFVYAVRRALMDGDLEVRETAAEAFDSLQSILGDRAVDDVLPQLLKLLESENQSEQALSALREIISRRSSTIFPVLIPTLIKKPVSAFNARALSSLAQVAGVTLNKRLPSILNALMESSLASTGDDLVALNGAIDKVNLSVKDQEGLQILMAHFYSFSESEDFRKRLFAAEHMLVFFQNCKLDYYRYVGDWVRHFITLFEDKSQDVVVAAVAAQNTLVSALRKDQLDSLVSIAYHSLRDVGSQGVNLPAFEVAQGVNSILPIFLYGLMHGTMDQREQSALGIADIVLKTEPSKLRPFVTQITGPLIRIIGERFPVEVKCAILYTLNIILSKISTFLRPFLPQLQRTFAKCLGDPSSEVIRSRAATALGTLITLQTRLAPIITELVSGARTPDAGVRKAMLNALFAVVSKSGQNMNEASAEAIEQLLDEISAESSEHMVICAKLYGALFSHLPDAQAKQLLESKVLSLEIQSEFSVLILNAAVKFGSQKIIELKLSDIVCSIISTASLQKEVTIAENGILALGKALLADIPQSFGNAKNLVEALKVNIEAPPSTSQDSRRLALLIIRVVSKENYSLIKPHISILAPAIFGCVRAIVIPVKLAAEAAFLALFQLVEDDSVLNKYIETLEGPRARSFVDYSRRVAVKLAAAERDRINSGSERVKLEEVEDLAEINAVGRDNEVSTNDP