The following text describes a protein:
Function:
A NHL-domain family protein that functions as a translational repressor to inhibit cell proliferation. Plays a central role in translation repression of hb mRNA by being recruited by nanos (nos) and pum to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR. Probably recruited by other proteins to repress translation of other mRNAs in other tissues. Negatively regulates expression of Myc in a 3'-UTR dependent manner in both neural progenitor and epithelial cells. Regulates expression of mei-P26, possibly at transcriptional level. Involved in the regulation of ribosomal RNA synthesis and cell growth. Participates in abdominal segmentation and imaginal disk development. During neuroblast division, segregates asymmetrically and inhibits self-renewal of one of the two daughter cells. Together with the asymmetrically segregating transcription factor prospero ensures that the daughter cell will stop growing, exit the cell cycle, and differentiate into neurons possibly by modulating the function of dm in ganglion mother cells (GMC). Restricts developmental potential of type II intermediary neuronal progenitor (INP) cells playing a role in proliferation and maturation of the neuroblasts.
Miscellaneous:
MISCELLANEOUS: The neuroblast overproliferation phenotype of brat knockout mutants is used as a well characterized brain tumor model. Early stages of tumorigenesis can be investigated because the tumor initiating cells of the type II neural stem cell lineage are known.
Subcellular Localization:
Cytoplasm. Cytoplasm, cell cortex. Note=In embryonic and larval neuroblasts, colocalizes with mira throughout mitosis using it as an adapter to segregate into one of the two daughter cells during asymmetric cell division. Accumulates at the apical cell cortex in prophase, forms a basal crescent in metaphase, and segregates into the ganglion mother cell (GMC). It is cortical in telophase but becomes cytoplasmic in interphase after mira is degraded.
Domain:
The NHL repeats form six-bladed beta-propeller that mediate the interaction with the pumilio repeats of pum, and are essential for translational effector function.
Sequence:
MASSPTPSLDSMRGGANSIESYEHAGYLSDSPLTLSGSSPPASDSAICSDEYTGGSSVKSRSEVTVINGHHPISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTSSSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCTACKSKCSDAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTSTGSTANESALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLLSATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDGSVVLASKDYRLYIYRYVQLAPVGM