The following text describes a protein:
Function:
Polyketide synthase; part of the gene cluster that mediates the biosynthesis of DIF-1 (Differentiation Inducing Factor-1), a signal molecule involved in the differentiation of pstO (prestalk-O) cells. The three-step process begins with the formation of (2,4,6-trihydroxyphenyl)-1-hexan-1-one (THPH) by the polyketide synthase StlB. THPH is then dichlorinated by the flavin-dependent halogenase ChlA. The last step of DIF-1 biosynthesis is the O-methylation of dichloro-THPH (or des-methyl-DIF-1) by the methyltransferase DmtA to yield DIF-1.
Miscellaneous:
MISCELLANEOUS: Encoded by one of the numerous copies of polyketide synthase genes localized in chromosome 5; MISCELLANEOUS: In reference to their hybrid nature and to their discovery in D.discoideum, authors term these type I FAS-type III PKS fusion enzymes 'steely'.
Domain:
Modular protein possessing six classical catalytic domains and a type III polyketide synthase domain. May facilitate covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization.
Sequence:
MNNNKSINDLSGNSNNNIANSNINNYNNLIKKEPIAIIGIGCRFPGNVSNYSDFVNIIKNGSDCLTKIPDDRWNADIISRKQWKLNNRIGGYLKNIDQFDNQFFGISPKEAQHIDPQQRLLLHLAIETLEDGKISLDEIKGKKVGVFIGSSSGDYLRGFDSSEINQFTTPGTNSSFLSNRLSYFLDVNGPSMTVNTACSASMVAIHLGLQSLWNGESELSMVGGVNIISSPLQSLDFGKAGLLNQETDGRCYSFDPRASGYVRSEGGGILLLKPLSAALRDNDEIYSLLLNSANNSNGKTPTGITSPRSLCQEKLIQQLLRESSDQFSIDDIGYFECHGTGTQMGDLNEITAIGKSIGMLKSHDDPLIIGSVKASIGHLEGASGICGVIKSIICLKEKILPQQCKFSSYNPKIPFETLNLKVLTKTQPWNNSKRICGVNSFGVGGSNSSLFLSSFDKSTTITEPTTTTTIESLPSSSSSFDNLSVSSSISTNNDNDKVSNIVNNRYGSSIDVITLSVTSPDKEDLKIRANDVLESIKTLDDNFKIRDISNLTNIRTSHFSNRVAIIGDSIDSIKLNLQSFIKGENNNNKSIILPLINNGNNNNNNNNNSSGSSSSSSNNNNICFIFSGQGQQWNKMIFDLYENNKTFKNEMNNFSKQFEMISGWSIIDKLYNSGGGGNEELINETWLAQPSIVAVQYSLIKLFSKDIGIEGSIVLGHSLGELMAAYYCGIINDFNDLLKLLYIRSTLQNKTNGSGRMHVCLSSKAEIEQLISQLGFNGRIVICGNNTMKSCTISGDNESMNQFTKLISSQQYGSVVHKEVRTNSAFHSHQMDIIKDEFFKLFNQYFPTNQISTNQIYDGKSFYSTCYGKYLTPIECKQLLSSPNYWWKNIRESVLFKESIEQILQNHQQSLTFIEITCHPILNYFLSQLLKSSSKSNTLLLSTLSKNSNSIDQLLILCSKLYVNNLSSIKWNWFYDKQQQQQSESLVSSNFKLPGRRWKLEKYWIENCQRQMDRIKPPMFISLDRKLFSVTPSFEVRLNQDRFQYLNDHQIQDIPLVPFSFYIELVYASIFNSISTTTTNTTASTMFEIENFTIDSSIIIDQKKSTLIGINFNSDLTKFEIGSINSIGSGSSSNNNFIENKWKIHSNGIIKYGTNYLKSNSKSNSFNESTTTTTTTTTTTKCFKSFNSNEFYNEIIKYNYNYKSTFQCVKEFKQFDKQGTFYYSEIQFKKNDKQVIDQLLSKQLPSDFRCIHPCLLDAVLQSAIIPATNKTNCSWIPIKIGKLSVNIPSNSYFNFKDQLLYCLIKPSTSTSTSPSTYFSSDIQVFDKKNNNLICELTNLEFKGINSSSSSSSSSSTINSNVEANYESKIEETNHDEDEDEELPLVSEYVWCKEELINQSIKFTDNYQTVIFCSTNLNGNDLLDSIITSALENGHDENKIFIVSPPPVESDQYNNRIIINYTNNESDFDALFAIINSTTSISGKSGLFSTRFIILPNFNSITFSSGNSTPLITNVNGNGNGKSCGGGGGSTNNTISNSSSSISSIDNGNNEDEEMVLKSFNDSNLSLFHLQKSIIKNNIKGRLFLITNGGQSISSSTPTSTYNDQSYVNLSQYQLIGQIRVFSNEYPIMECSMIDIQDSTRIDLITDQLNSTKLSKLEIAFRDNIGYSYKLLKPSIFDNSSLPSSSSEIETTATTKDEEKNNSINYNNNYYRVELSDNGIISDLKIKQFRQMKCGVGQVLVRVEMCTLNFRDILKSLGRDYDPIHLNSMGDEFSGKVIEIGEGVNNLSVGQYVFGINMSKSMGSFVCCNSDLVFPIPIPTPSSSSSSNENIDDQEIISKLLNQYCTIPIVFLTSWYSIVIQGRLKKGEKILIHSGCGGVGLATIQISMMIGAEIHVTVGSNEKKQYLIKEFGIDEKRIYSSRSLQFYNDLMVNTDGQGVDMVLNSLSGEYLEKSIQCLSQYGRFIEIGKKDIYSNSSIHLEPFKNNLSFFAVDIAQMTENRRDYLREIMIDQLLPCFKNGSLKPLNQHCFNSPCDLVKAIRFMSSGNHIGKILINWSNLNNDKQFINHHSVVHLPIQSFSNRSTYIFTGFGGLTQTLLKYFSTESDLTNVIIVSKNGLDDNSGSGSGNNEKLKLINQLKESGLNVLVEKCDLSSIKQVYKLFNKIFDNDASGSDSGDFSDIKGIFHFASLINDKRILKHNLESFNYVYNSKATSAWNLHQVSLKYNLNLDHFQTIGSVITILGNIGQSNYTCANRFVEGLTHLRIGMGLKSSCIHLASIPDVGMASNDNVLNDLNSMGFVPFQSLNEMNLGFKKLLSSPNPIVVLGEINVDRFIEATPNFRAKDNFIITSLFNRIDPLLLVNESQDFIINNNINNNGGGGDGSFDDLNQLEDEGQQGFGNGDGYVDDNIDSVSMLSGTSSIFDNDFYTKSIRGMLCDILELKDKDLNNTVSFSDYGLDSLLSSELSNTIQKNFSILIPSLTLVDNSTINSTVELIKNKLKNSTTSSISSSVSKKVSFKKNTQPLIIPTTAPISIIKTQSYIKSEIIESLPISSSTTIKPLVFDNLVYSSSSSNNSNSKNELTSPPPSAKRESVLPIISEDNNSDNDSSMATVIYEISPIAAPYHRYQTDVLKEITQLTPHKEFIDNIYKKSKIRSRYCFNDFSEKSMADINKLDAGERVALFREQTYQTVINAGKTVIERAGIDPMLISHVVGVTSTGIMAPSFDVVLIDKLGLSINTSRTMINFMGCGAAVNSMRAATAYAKLKPGTFVLVVAVEASATCMKFNFDSRSDLLSQAIFTDGCVATLVTCQPKSSLVGKLEIIDDLSYLMPDSRDALNLFIGPTGIDLDLRPELPIAINRHINSAITSWLKKNSLQKSDIEFFATHPGGAKIISAVHEGLGLSPEDLSDSYEVMKRYGNMIGVSTYYVLRRILDKNQTLLQEGSLGYNYGMAMAFSPGASIEAILFKLIK