The following text describes a protein:
Function:
Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing. Requires a 3'-single-stranded tail as entry site for acid nuclei unwinding activities as well as the binding and hydrolyzing of any of the four ribo- or deoxyribo-nucleotide triphosphates (NTPs). Binds to DNA, RNA and small interfering siRNA. Plays a role in DNA replication at origins of replication and cell cycle progression (By similarity). Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors. Plays several roles in post-transcriptional regulation of gene expression. Promotes pre-mRNA alternative splicing activities of a subset of genes. As component of a large PER complex, is involved in the negative regulation of 3' transcriptional termination of circadian target genes. Component of the coding region determinant (CRD)-mediated complex that promotes cytoplasmic MYC mRNA stability. Plays a role in mRNA translation. Also plays a role in the RNA-induced silencing complex (RISC) loading complex (RLC) assembly, and hence functions in the RISC-mediated gene silencing process. Mediates the attachment of heterogeneous nuclear ribonucleoproteins (hnRNPs) to actin filaments in the nucleus (By similarity).
Subcellular Localization:
Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Nucleoplasmic shuttling protein. Its nuclear import involves the nucleocytoplasmic transport receptor Importin alpha/Importin beta receptor pathway in a Ran-dependent manner. In interphase, localizes in nuclear stress granules and at perichromatin fibrils and in cytoplasmic ribonucleoprotein granules. Excluded from the mitotic nucleus as early as prophase and re-entered the nucleus at telophase. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Domain:
DRBM domains cooperate for the binding to nucleic acid but not for unwinding helicase activity. The helicase-associated domain-2 (HA2) region is essential for the duplex RNA unwinding helicase activity. The minimal transactivation region (MTAD) mediates interaction with the RNA polymerase II holoenzyme and stimulates transcriptional activation. The oligonucleotide- or oligosaccharide-binding (OB-fold) and the repeated arginine and glycine-glycine (RGG) regions are dispensable for both RNA-binding and unwinding helicase activities. The RGG region contains both nuclear localization signal (NLS) and nuclear export signal (NES) and is necessary and sufficient for nucleocytoplasmic shuttling in a RNA-independent manner.
Sequence:
MSRDVKEFLYAWLGKNKYGNPTYDTKSETRSGRQRFKCELRITGFGYTAFGNSTNKKDAATNAAQDFCQYLVREGKMQQSDIPTLTSSSLEASSTWQDSETATMFCGGEDGNSFQESQQPIPQKRFPWSNNAYQRNEGTHEQYITQKAEEIAASETVDLKSEIHGGWTMENSKKALNEFLQKMRLPQVNYGTKIRESNTVKTMETTAQIFVPQINKNLVGKGTGSNKKVSEAACAMNIVRQMFHLNIMQSYSGPIKKSKVSTLPEIAISIPEDLSTRVTEYVRSCGLELPEINETSATPEAPTSLLTDVKLAQFPVSEICSASNISWAPPLQNWNPWRASNIDEEPLAFMSMEQISQRIMEKEDFKRGEALDKITAQRGELPVAQYRENIVQTVAENRVTLIKGETGCGKSTQVAQFLLESFLENSNGASFNAVVSQPRRISAISLAERVANERGEEVGETCGYNVRFDSATPRPYGSIMFCTVGVLLRMMENGLRGISHVIIDEIHERDVDTDFVLIVLREMISTYRDLRVVLMSATIDTDLFTNFFSSIPDVGPTPVITMHGRTFPVQSFYLEDILHNLQHMPEEPDQKKRKKGGPPPPDDDEGDEEVDDKGRNMNILTDPSINESLKTAMSRISEKDIPFGVIEAILNDIASRGVDGAVLVFLPGWAEIMTLCNRLLEHQEFGQANKYEILPLHSQLTSQEQRKVFNHYPGKRKIIVSTNIAETSITIDDVVYVIDSCKAKERMYTSNNNMVHFATVWASKTNVIQRRGRAGRVRAGYAFHLCSKMRFEALDDHGTAEMLRIPLHQIALTIKLLRLGSVGEFLGKALQPPPYDMVVESEAVLQAMGALDRNLELTSLGKMLARMPIEPVIAKVLILGTALGAGSVMCDVASAMSFPTPFVPREKHHSRLSGTQRKFAGNKFSDHVAIVSVIQGYREAVQMGASAAEREFCERYSLSNPVLKMTDGARRQLIDVLRNQCSFPEDILFDISVNVNGPDRELNLMRSLLVMALYPNVAYYVGKRKVLTIEQSSALINKYSMLVPMNNRQEMDFPSPLLVFTEKVRTRCISCKQMSVISAIQLLVFGSRKVECVGEGLVRIDETITIRMNVSTAAALIGLRPCIEALLVKSCENPESLAGLNPSDAELRQLLRDISSEEFMSEAGPIKDSLLTDNALIQKGPAPNRLEYADWGPSNNNSSFQNQDFPPAAGGKVHPYRGNRRGNHPYAQNRPYAPPNSGMGYQNFNNSGYGASGDWNAGRGNYGNVGSGYRGAGGGGGYRGGRGGRGGGRGGGGRGWNASNW