The following text describes a protein:
Function:
Component of an extracellular matrix cue that is involved in the guidance of dorsoventral midline migrations and in the specification of postsynaptic domains at neuromuscular junctions (NMJs). Acts as a ligand for the netrin receptor unc-40 and the neuroligin receptor nlg-1. Secreted by the dorsal and ventral nerve cords to attract sensory axons and muscle membrane extensions called muscle arms. In parallel with unc-6 and slt-1, involved in the netrin receptor unc-40 dependent guidance of the AVM and PVM mechanosensory axons along the dorsal-ventral axis. The unc-40 coreceptor eva-1 is enhancing the responsiveness of unc-40 to the madd-4 guidance cue to attract the muscle arm extensions and AVM mechanosensory axons towards the dorsoventral midline. Acts as a synaptic organizer and is required for the specification of inhibitory GABAergic and excitatory cholinergic identities of postsynaptic domains at neuromuscular junctions (NMJs). Required for the recruitment of unc-40 to both cholinergic and GABAergic NMJs. Promotes the clustering of ACh receptors and GABA(A) receptors at postsynaptic sites during synaptogenesis. The binding to the presynaptic adhesion protein nrx-1 and to the neuroligin nlg-1 at postsynaptic sites promotes clustering of GABAergic receptors at postsynaptic NMJs, thereby contributing to normal GABAergic synaptic transmission; Isoform a and isoform c: Promotes the clustering of acetylcholine receptors (AChR) at excitatory cholinergic synapses of NMJs via the netrin receptor unc-40; [Isoform b]: Acts as a guidance cue in the attraction of muscle membrane extensions (muscle arms) to the dorsal cord and in cooperation with unc-6 to the ventral cord via the netrin receptor unc-40 and via the unc-40 coreceptor eva-1. Together with nrx-1, clusters netrin receptor unc-40 and neuroligin nlg-1 at postsynaptic sites of GABAergic NMJs, thereby promoting the recruitment of GABA(A) receptors at GABAergic synapses. Prevents the recruitment of GABAergic receptors to cholinergic synapses.
Miscellaneous:
MISCELLANEOUS: [Isoform b]: Produced by alternative promoter usage; MISCELLANEOUS: [Isoform c]: Produced by alternative splicing of isoform a.
Subcellular Localization:
Cell projection, axon. Secreted. Synapse. Secreted, extracellular space, extracellular matrix. Note=Isoform b is secreted by cholinergic and GABAergic motoneurons and localizes to excitatory cholinergic and inhibitory GABAergic NMJs, whereas isoform a and isoform c are exclusively secreted by cholinergic motoneurons and localize to excitatory cholinergic NMJs.
Domain:
The Ig-like C2-type domain is required for the attraction of the muscle arm extensions.
Sequence:
MKCSYTVVFLLFYLLIASFHVDALSWAAWSPWSSCTKTCGGGVSRQLRRCLTSKCSGESVRFKVCAQKTCESKSRLARDTICGGEEIVSRGQCEVVCRSRLTGANFLWRVDDGTPCQAATSRAVCSKGSCQIVGCDGLISSSFRFDACGVCGGRGDTCDNGKFIWKVSEEYTACASNCDDIVDWSGAGRSIASTSQPIVVCVNAITGRVVPEKLCADKLRPKVEARPCPMLICPSRCRWMAADWTECVPHCGEGTRKREVYCVQTAHNVTVHVPDTFCENGTRPAAEENCVSTSCGRWEAGKWSKCTASCGQGVRRRHVACVGGSDCDEGGRPRQETTCYAGIPCSIATNSLDWNDRAYLDGNTFGSMDNHNDWQAPRLVAGEWSTCSSTCGTGVMSRTVECVAVNPISSAPIKLPMSECQDQEQPKLFESCEVRSCPLQEDSKLSEDEAPYQWRYGDWTQCSASCLGGKQKAALKCIQVSTGKSVQWSQCDARRRPPEKSRPCNQHPCPPFWLTSKYSDCSMSCGSGTARRSVKCAQTVSKTDGADAHIVLRDDRCHFKKPQETETCNVVACPATWVTAQWTECSRSCDSGERRRQVWCEIRDSRGKTQRRPDVECDANTKPQTVEVCSFGSCSRPELLSNRVFEQNAEQKKLTLGIGGVATLYQGTSIKIKCPAKKFDKKKIYWKKNGKKIKNDAHIKVSANGNLRVFHARMEDAGVYECFTDRLQGNVTLNFKYRDFPASRVDLAPKPQIPSTKNRQRVQVSKEDVLREQASVLHKMNVSLIEALLTAPNDEKAREQLRKYGNELVARWDIGHWSECRQKTCHVAGYQARGISCKVTFHGEIRNVDNSICESLASVRPPETRPCHREDCPRWEASQWSECSSQRCVSSMLAQKRRNVTCRFTNGTSVDIQHCDITNRPATTMDCPNQNCKAEWRTSDWGSCSSECGTGGVQLRLLSCVWISSGRPAGRNCEQMRRPHSARACVADEPLPPCMPTASALYQRDASCQDQSRFCDIIKLFHSCDSLEVRQKCCSTCTFVERKKF