The following text describes a protein:
Function:
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrate. Required for regulation of cell proliferation in imaginal disks and germ cells. Acts as a negative regulator of hh, ci and dpp expression in the anterior of the eye disk. Acts as a positive regulator of the canonical Wnt signaling pathway by mediating ubiquitination and degradation of gro. Catalyzes 'Lys-63'-linked polyubiquitination of akirin, thereby activating the immune deficiency pathway (Imd).
Miscellaneous:
MISCELLANEOUS: A cysteine residue is required for ubiquitin-thioester formation.
Subcellular Localization:
Nucleus. Cytoplasm.
Sequence:
MVSMQFVLQPLPGSDDQFIERIREVSDKVNRFGYGSHRIFEQLKIPVKEVVIGPAHIGVLLEDGKAFRVSFSINSEKLDLTKSDAKCSTSGGSGTASASKAPSSSRPMARSRARLLRATGRSNSTGQGSGSRSTGVIIGGSTSSRPLVTVPATYVPEELISQAEVVLQGKSRNLIIRELQRTNLDVNLAVNNLLSRDDEEAEDTEEGADNYVPEDLISLLDNGFSGDNNSVIIDPSDGLFSEEIFSNYSSIRNLLFDRIRSERSNANANAADSNQSTTRSTSSGTALTGNSGLSAQISVNADREAFSRWRDRQYYGPRRWISKDDYTWEKDADSKKKEPSPMLSPIWISEELQPWPEKSSVRFKTIGALYSEFIALSESGDLYQWRWSDAEPYKSETENVYHPKTVSLNIVERVELISANFIRCSVVTETNRVATWMDEQLGYIGAKLEHSCCAFNEFISDSITKIYVCSLYTVVKTESNNIYWWGVLPFDQRRFLWDKFRTKTKKPFKVVATDINVGAQVIMKKCPMYQSGSIGFTCSNGVPKVGQLLNSVWTFTDVCRMKIININTNSGVDKSQAAGNNLNAHGITPDKDLPKSTAMPSTGSSKNGQSFSNSKESTDRIDMPPPPSPASSTCSDTGSVTSHKRTKRATTKEDSNAPQEGRKDEELWEVKDVVFVEDKVGPVGKVLKVDGDFVAVRFPAINAAAVAAAAAATSSTSNTASTSKEEGKEDDWQQCRLLRREDVQIFRTAMSTRGPDWLQKQPKKINVGGDAAGAQILTLAVDSRGIHVIKKVLGKIHYSLYNLYNCKQEQNCLFPTDCNSFIGSTPGNILMACNDDCSGNSSTIVLRDGNGALYPLAKDCLGSIKDPQWFDLPPVKSITMSTISLPAMLSGVNLKSKVCMTALLFDTQKLMPHILRCDVKNSFAALGRLEREDQADTALVVEERCDGARNIFHACVIMCAPSSNKDSPPDSPSGGVEKKSLVGLSVARSIPTVSTSAYVSSIAFGASASSSNENSSFATMSSSAAGSASSTSRDNRTNLRDMMNRLINSDQAEQSGSQPMATNNEDHAYIPWPAETPAASNLSASSSQNVSDSIEDDISKIIPSSSQSSMLSNIKLGSPTYTFDLAQRREHALTILQQMCVSPALRPYLCHMLSTKDAQGQTPFMLSVSCRAYEAGIILLNTILMLSEQDPQLKEAMIFPNGSPADQSPLHVICYNDTCSFTWTGADHINQNIFECKTCGLTGSLCCCTECARVCHKGHDCKLKRTAPTAYCDCWEKCKCKALIAGNLTKRFALLCKLVSCTDLVTKFNSKGESILLFLIQTVGRQIVEQRQYRFSVRVRNVSTAATGATGNNSVISNRKTSAAEIDNDMPDHDLEPPKFARKALERLLIDWNAVRSMIMSGAERGDVPNPAGSASENSNSEGFNMFIQTQHGSTLLDKFTHSLIVKCTSDHLDTLLLTLVRELQNASVSNRSKEAEEVVRRFVRSVARVFVIFNLEKQPNPEKRKSHSSCNKYVQSCVKVFQTLHKISIEELCEVSEALIAPVRLGVVRPTAPFTMSSSNLDNSDDLFSVDPLAPSNVESPSEQILVHDAGNDQSANFNIQQNYDVVAMETIRDASESEEVINREANSHNQDDELIENQRNEDGMQDDESDNDFTFNDAETESDSDDNQSNQEVQRSVQAGATVGSENDIGVLFLEDESGDSSAQEEDGSEDGESDDQSDEFNFNEQQLERRSTNSNARSDLAPQTMQWAIRSRDTARSSVRVPTGSNMVFIDPMALRRSTVPASTTVTTPSIEPHTMATTASNLARAFGITIRQISELISILSYNVLNDIETSLKIQNDEAIAVQAFVEKRLKATWDWMFTVMDGTEAQLKFGAYLTNYTDPNHPLHPLNLSAQASSSQTPAPATSSSVNGVNIMGSNSRRDFFTYCLSLMRSHTSEHRDALPVLDITALRHIAYVLDAFVYYMRNDSGFYDKQDTISGRINNLSPMTESYDTDDELANLEEFNADVQMSASSMPSGSQGTRRHAFFARSESTLSLGCSAPEGFELPLDMAMPLADKPHLLQPNSKRQELFANLPLLVTTNANNSGATNDGDGGSIFDYTPTRLGFSNSLKRNERVYETVPIDSSKTGDGNVTNKAEGSTDSNIYVQLKKKQGSDDFKSHKEADGNQSKYEKVVLMETDDSLPSTSKSTEALMATRPEVIIAPNKASVSPATAARSVIVLAGGSCLKTIDSDINNYSASNLSTAEQAKCDTQYQKSTSDHLLLFPARGSQFYQSNFSELPSWNFLLSRWKLTLDLFGRVFMDDVGMEHGSVLPELRGFPVKEMRFRRHMEKLRNGQQRDLVLCKLERNRESLIVQTFKELNTQFGNQSRRIQPPITFNRVKVTFKDEPGEGSGVARSFYTSIAEALLASAKIPNLESVQVGTNHSKYVVPFSSILRSRTVSGSSRDQSTLQRRGSNSKILWRSARERKALNLDARPYTPPNSSDNATPESLNDHLSVHLQQIGERLYPKIHSINQTHAPKITGMLLEIPTPQLLSVISSDETLRQKVNEAIEIITFKQKSETSAQSSQPKKSPSVVVVDPVDDDNEPLFYSPGKRGFYTPRQGFASFERINAFRNIGRLIGLCLLQNELLPLFLQRHVLKYILGRKIKFHDLAFFDPALYESFRQIIQNAQTKEGEETINRMELCFVIDLMKEEGCGNRELIPGGRDVAVTSSNIFEYVRRYTEYRLIKSQEKALEALKDGVFDVLPDNSMINLTAEDLRLLLNGVGDINVSTLISYTTFNDESSEGPDKLLKFKKWFWSIVEKMNIMERQDLVYFWTGSPALPASEEGFQPLPSVTIRPADDSHLPTANTCISRLYIPLYSSKSILRSKMLMAIKSKNFGFV