The following text describes a protein:
Function:
Mediates bacterial adherence to host endothelial cells and host extracellular matrix proteins (collagen type I, III, IV, laminin and fibronectin). Static versus dynamic adherence results differ slightly; in dynamic adherence studies bacteria bind to fixed components under a constant defined flow rate to simulate in vivo infection conditions. Induces secretion of host proangiogenic cytokines such as VEGFA, ADM, IGFBP-3 and IL-8. May prevent bacterial phagocytosis by macrophages (Probable). Probably mediates bacterial autoagglutination. Negatively impacts type IV secretion system effectors (VirB/D4 T4SS and its substrate Bep proteins), possibly by preventing close association of host and bacterial cells. This implies the 2 factors are expressed at different times during infection (Probable).
Miscellaneous:
MISCELLANEOUS: Infection with B.henselae leads to cat scratch disease in immunocompetent individuals and bacillary angiomatosis, tumorous proliferations of endothelial cells in the skin and internal organs in immunocompromised patients.
Subcellular Localization:
Cell surface. Cell outer membrane. Note=Forms long (about 240 nm) filaments extending from the cell surface, which were initially called type IV pili. The C-terminal translocator domain is localized in the outer membrane and the passenger domain is at the cell surface (By similarity).
Domain:
The N-terminus (residues 48-376) performs most of the host-associated functions of the protein and binds collagen. The large central domain (composed of neck-stalk repeats about residue 470-2850) is required to bind host fibronectin and contributes to host cell adherence, collagen-binding and induction of host proangiogenic factors; The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface.
Sequence:
MKKLSVTSKRQYNLYASPISRRLSLLMKLSLETVTVMFLLGASPVLASNLALTGAKNLSQNSPGVNYSKGSHGSIVLSGDDDFCGADYVLGRGGNSTVRNGIPISVEEEYERFVKQKLMNNATSPYSQSSEQQVWTGDGLTSKGSGYMGGKSTDGDKNILPEAYGIYSFATGCGSSAQGNYSVAFGANATALTGGSQAFGVAALASGRVSVAIGVGSEATGEAGVSLGGLSKAAGARSVAIGTRAKAQGEESIAIGSSVKNGDKDGSAVAQGAKAIAIGSNSISFQHYAVAVGAKAHALLSKTVALGYDSVADVDAGIRGYDPVEDEPSKDVSFVWKSSLGAVSVGNRKEGLTRQIIGVAAGTEDTDAVNVAQLKALRGMISEKGGWNLTVNNDNNTVVSSGGALDLSSGSKNLKIVKDGKKNNVTFDVARDLTLKSIKLDGVTLNETGLFIANGPQITASGINAGSQKITGVAEGTDANDAVNFGQLKKIETEVKEQVAASGFVKQDSDTKYLTIGKDTDGDTINIANNKSDKRTLTGIKEGDISKDSSEAITGSQLFTTNQNVKTVSDNLQTAATNIAKTFGGGAKYEDGEWIAPAFKVKTVTGEGKEEEKRYQNVADALAGVGSSITNVQNKVTEQVNNAITKVEGDALLWSDEANAFVARHEKSKLGKGASKATQENSKITYLLDGDVSKDSTDAITGKQLYSLGDKIASYLGGNAKYEDGEWTAPTFKVKTVKEDGKEEEKTYQNVAEALTGVGTSFTNVKNEITKQINHLQSDDSAVVHYDKNKDETGGINYASVTLGKGKDSAAVTLHNVADGSISKDSRDAINGSQIYSLNEQLATYFGGGAKYENGQWTAPIFKVKTVKEDGEEEEKTYQNVAEALTGVGTSFTNIKSEITKQIANEISSVTGDSLVKKDLATNLITIGKEVAGTEINIASVSKADRTLSGVKEAVKDNEAVNKGQLDKGLKHLSDSLQSDDSAVVHYDKKTDETGGINYTSVTLGGKDKTPVALHNVADGSISKDSHDAINGGQIHTIGEDVAKFLGGAASFNNGAFTGPTYKLSNIDAKGDVQQSEFKDIGSAFAGLDTNIKNVNNNVTNKFNELTQNITNVTQQVKGDALLWSDEANAFVARHEKSKLGKGASKATQENSKITYLLDGDVSKDSTDAITGKQLYSLGDKIASYLGGNAKYENGEWTAPTFKVKTVKEDGKEEEKTYQNVAEALTGVGASFTNVKNEITKQINHLQSDDSAVVHYDKNKDETGGINYASVTLGKGKDSAAVTLHNVADGSISKDSRDAINGSQIYSLNEQLATYFGGGAKYENGQWTAPIFKVKTVKEDGEEEEKTYQNVAEALTGVGTSFTNIKSEITKQIANEISSVTGDSLVKKDLATNLITIGKEVAGTEINIASVSKADRTLSGVKEAVKDNEAVNKGQLDTNIKKVEDKLTEAVGKVTQQVKGDALLWSNEDNAFVADHGKDSAKTKSKITHLLDGNIASGSTDAVTGGQLYSLNEQLATYFGGGAKYENGQWTAPTFKVKTVNGEGKEEEQTYQNVAEALTGVGASFMNVQNKITNEITNQVNNAITKVEGDSLVKQDNLGIITLGKERGGLKVDFANRDGLDRTLSGVKEAVNDNEAVNKGQLDADISKVNNNVTNKFNELTQNITNVTQQVKGDALLWSDEANAFVARHEKSKLEKGVSKATQENSKITYLLDGDISKGSTDAVTGGQLYSLNEQLATYFGGGAKYENGQWTAPTFKVKTVNGEGKEEEQTYQNVAAAFEGVGTSFTNIKSEITKQINNEIINVKGDSLVKRDLATNLITIGKEIEGSVINIANKSGEARTISGVKEAVKDNEAVNKGQLDTNIKKVEDKLTEAVGKVTQQVKGDALLWSNEDNAFVADHGKDSAKTKSKITHLLDGNIASGSTDAVTGGQLYSLNEQLATYFGGGAKYENGQWTAPTFKVKTVNGEGKEEEKTYQNVAAAFEGVGTSFTNIKSEITKQIANEISNVTGDSLVKKDLDTNLITIGKEIAGTEINIASVSKADRTLSGVKEAVNDNEAVNKGQLDANISKVNNNVTNKFNELTQSITNVTQQVKGDALLWSDEANAFVARHEKSKLEKGVSKATQENSKITYLLDGDISKGSTDAVTGGQLYSLNEQLATYFGGGAKYENGQWTAPTFKVKTVNGEGKEEEQTYQNVAAAFEGVGTSFTNIKSEITKQINNEIINVKGDSLVKRDLATNLITIGKEIEGSVINIANKSGEARTISGVKEAVKDNEAVNKGQLDTNIKKVEDKLTEAVGKVTQQVKGDALLWSNEDNAFVADHGKDSAKTKSKITHLLDGNIASGSTDAVTGGQLYSLNEQLATYFGGGAKYENGQWTAPTFKVKTVNGEGKEEEKTYQNVAAAFEGVGTSFTHVKNEITKQINHLQSDDSAVVHYDKDDKNGSINYASVTLGKGKDSAAVALHNVADGSISKDSHDAINGGQIHTIGEDVAKFLGGDAAFKDGAFTGPTYKLSNIDAKGDVQQSEFKDIGSAFAGLDTNIKNVNNNVTNKLSELTQNITTVTQQVKGNALLWSDEANAFVARHEKSKLEKGASKAIQENSKITYLLDGDVSKGSTDAVTGGQLYSMSNMLATYLGGNAKYENGEWTAPTFKVKTVNGEGKEEEQTYQNVAEALTGVGTSFTNIKSEIAKQINHLQSDDSAVIHYDKNKDETGTINYASVTLGKGEDSAAVALHNVAAGNIAKDSRDAINGSQLYSLNEQLLTYFGGDAGYKDGQWIAPKFHVLQFKSDGSSGEKESYDNVAAAFEGVNKSLAGMNERINNVTAGQNVSSSSLNWNETEGGYDARHNGVDSKLTHVENGDVSEKSKEAVNGSQLWNTNEKVEAVEKDVKNIEKKVQDIATVADSAVKYEKDSTGKKTNVIKLVGGSESEPVLIDNVADGKIEADSKQAVNGGQLRDYTEKQMKIVLDDAKKYTDERFNDVVNNGINEAKAYTDVKFEALSYTVEEVRKEARQAAAIGLAVSNLRYYDIPGSLSLSFGTGIWRSQSAFAIGAGYTSEDGNIRSNLSITSSGGQWGVGAGITLRLK