The following text describes a protein:
Function:
Non-reducing polyketide synthase; part of the pki gene cluster that mediates the biosynthesis of 2,4-dihydroxy-3-methyl-6-(2-oxoundecyl)benzaldehyde. The first step in the pathway is the generation of the decanoyl starter unit by the FAS composed of subunits pkiB and pkiC, which is then transferred directly from the FAS to the SAT domain of the non-reducing polyketide synthase pkiA. PkiA condenses the decanoyyl starter unit with 4 malonyl-CoA units and performs one methylation step to yield 2,4-dihydroxy-3-methyl-6-(2-oxoundecyl)benzaldehyde.
Domain:
Multidomain protein; including an N-terminal starter unit:ACP transacylase (SAT) domain, a beta-ketoacyl synthase (KS) domain, a malonyl-CoA:ACP transacylase (MAT) domain, a product template (PT) domain that controls the immediate cyclization regioselectivity of the reactive polyketide backbone, an acyl carrier protein (ACP) domain, a methyltransferase (CMeT) domain responsible for the incorporation of methyl groups, and a reductive NADPH-binding domain that is required for NADPH-dependent product release.
Sequence:
MVTPAASQDPPAIPARQNASATAAMAVNAKDTVEQERNVVLLFGCQWLTFTASDFRQLRKAVLDNPELHWMLDVLSELPGYYRAAAGTSCVPSLRAIRGEEDLRELERWFRCDDLSTAKFPLCYTQLAPLLMMTHFVQYSQWLKMQPNGRNPVVEIVGFCIGLLSSIAVSATRMGSLKMYGSVAMRLAMLLGAMGDLQQAGEEYTSLAIGWKRPELEDELPAERALTEQSYITVQYDENRATIMAPRRSVAALQQTLQSAGFSANAVEYNGRYHWPGHEKSLTPLIHLCNTHSGLQLPDASELLHPPRANSTAEPVRSGCLHELVLRAVLAQQCLWHKTFSAVYREHLTTPSSIVVEFGPERCVPPTLFRRLPQRIVHFADVELPATISRDHELATRPPAETDIAIVGMACRVAGADDLDEFWDLLCSGQSQHREMPRERYANYETPWRPEASHRSWLGNFVRDIDAFDHKFFRKSPREAMSQDPQQRLMLQVAYQALESAGYFSQPSPGKDIGCFIATCTVDYEHNVNCHPASAYAATGLLRSFLAGKLSHHFGWRGPSLCVDTACSGSAVALHHACRAILSGDCTAALVGGANAITSPLAYDNLAGASFLSPTGPCKPFDAKADGYCRGEGFAAIYIKKLSHAIADGDQVLATIASTAVEQNDNCTPIVVPDTASLAGLFKKVTQRAHLHSRDISIVEAHGTGTQAGDPAEYESVRDVLGGPRRVGNLALGSVKGLVGHTEGVSGIIALCKVVLMILNGQIPPQPGFHSLNPHIRAMPDDHIEIGTRVKPWEVGFRAALINNYGACGSNASMVITQGPQKDEVQERGIHAENVALPFRVCGLDKARLQAYAARLRRFLSRSERGISFANIAFNLTRKSNPALECQCVFQTRSESELKDILTGLEEGDNKYIIQVKKPKRPLVLCFGGQVGRSIGLDRTFYNAFPLFKHHLDSCDDILKANGDSSIYPGIFATAPVLDIVQLHTQLFALQYACARSWMDCGVEVTAVIGHSFGELTALCISGALSLPDALTLIVRRAVLIRDKWGADPGAMLAVEGDRSTLEKHLESSSANIACFNGPRSFTVAGPTAVIDFLQEELGADSAFRLKRLEVTNAFHSTLVDPLLPALASAIDGLALNTATIPIERATEHQAADTIPLSIVADHLRQPVYFNNAVQRLAARHGPAIWLEAGSNSTITSLARRALGLGVSGNTFHSVNVTSTSALMNLTDVTVGLWSDNVPCTFWGYHARQTREYAPLLLPPYQFERTRHWMENKPLPLKYNQAQAVMEVSGHTAAKTAPIAPATLLLDYAIELLRSLPNNQRKIPRVFDVGSDAPLLLDSNREVWIEVSAEDDKRTWALRFQSQTKGGQSDSRLLHCTAHISMHDVRCSRLQTEFTQYARLVSHARCADLLTDPEVDDILQGRNVYRSFAEIVEYSEQYQGVKRLVGKGRESAGRVVKSYSGKTWADPFLCDSFSQCAGFWVNCMTDRAEDEVYVASGIEQWMRTPLYADMATARPDTWHVWARHQQSEGLYTSDVFVFTPDGELVEMFLGLRYSRVAKSLFTRLLRGSTLKVDCRTKDTANQENNSIKDLVSRVKAVVAEICAVKPSEIQDDSHLADAGVDSLMAMELARELEVAFKCTIALEALVEAETFHDLVQAVQSALGETYEDSSVCSGNQCSTTDEATEFPSTSWSITSVSDTADLVLPLDGVLDALDETKGLTDQFLADNKCSGRLLNFTPLMVEMCIVLTLEALEELGSNIRSARANDRLPRIEFDTQHGPLVEYLYGRLLEAGLIKLDGSTVIRTEICAPTESSSTLLHKIEREYPEYGGASKLTFYTGSRLASVLRGEQDGLQLIFGTAEGQRLVSWMYGDEPHNVAGYKLMGEFIRRLVDKLPPAAAREGMTLRILEMGAGTGGGTKWMLPLLAALPVPVEYTFSDISPAFLAQARRKFRDYQFVRYCVHDIEKPPSEDLGKYHIIMASNAVHATSNLQVSTGNMRQALRPDGVLMLLEMTRPVFAIDLVFGLFRGWWVFNDGRTHAITNEQRWKDDLQAVGYGHVDWTDGESNEVGVQRVIFATAGGEQYHPVSPQEDAARLRTVVEYVYQHTAGFTMPALPPRIRAPANHACILVTGATGSLGSHLVARLVQLSNVQAVICLNRVSRMGPRVRQKEAVAARGLSLESKEETKLMVIETDTANDRMGLSVEQCRYLQENVTHIIHNAWPMNGAAPLSKFEGQFRALRNLIDLARCIATAQRHPVRFQFISSIGTVNGGGALEERTRIEQVMSNGYNEAKFVCERMIHETLQRYPAVFQATIVRPGQISGSEETGYWNTAEHFPAMVKSSQSLGAFPSLAGRLGWTPVDVAARIIAELLLDEGIPEEIYHVDHPTGQNWTTVVDVLAEELEATEVPFKDWIQRVRNRGGSRENPAGFMADWLETNFERMSCQGPLDTRVARRHSKTLREMGGGGGDEHVRRVVRSWKECGFLTQAQTRQGIP