The following text describes a protein:
Function:
RNA binding protein and post-transcriptional regulator of mRNA translation; can act as a repressor or activator of gene expression. Binds and represses translation of several mRNAs in a miRNA and 3'-UTR-dependent manner, possibly in association with components of the RNA-induced silencing complex (RISC). Represses expression of brat in a RISC-independent manner, possibly in coordination with nanos, to derepress the BMP signaling pathway. Binds nanos mRNA and involved in bam-bgcn mediated repression of nanos mRNA translation. Regulator of stem cell proliferation. Promotes both germline stem cell (GSC) self-renewal and downstream GSC-lineage cell differentiation. Represses the microRNA pathway in cystocytes inhibiting expression of a subset of microRNAs involved in differentiation. Involved in a regulatory cascade with bam to control the progression of cystocytes through transit amplification and the switch to spermatocyte differentiation; mei-P26 facilitates bam accumulation, which in turn represses translation of mei-P26. Negatively regulates the levels of Myc protein in a proteasome-dependent manner; involved in maintaining tissue homeostasis by regulating cell and tissue growth and Myc-induced apoptosis.
Subcellular Localization:
Cytoplasm. Cytoplasm, Cytoplasmic ribonucleoprotein granule. Note=In male germline cells colocalizes in cytoplasmic puncta with gw/GW182, a component of RISC (the RNA-induced silencing complex).
Domain:
The C-terminal region containing the NHL repeats is required for interaction with AGO1 and for regulating ovarian germline stem cell proliferation. The C-terminal NHL repeats are also required for regulation of brat expression. The C-terminal NHL domain binds single stranded RNA in a sequence-specific manner; preferentially binds uridine-rich RNA with at least 4 U residues followed by an ACA anchor sequence (5'-UUUUACA-3'). The NHL domain is essential, but not sufficient, for post-transcriptional regulation of mRNA translation. The NHL domain is required for proteasomal-dependent regulation of Myc protein levels; The N-terminal RING-type Zinc finger domain is not required for post-transcriptional regulation of mRNA translation.
Sequence:
MTTLEEPVQAADTVDPCPPGIPNLKTVLSDDASNSSVLEDIDVSNTSSSSSSSSSSSSSSGSCSGSGTDTDTGKGSSNVGSLDVTSSSSIGSETSISSTGSTAIAGPSTLLDDSNSSGLGLVVDVDSNGSSQELSSHGVTELDSLEDSCSGLMIKDGDKLCKLCSLRLVMPRILSCLHVFCEDCLQAVVSKDTMSASSPNSCSSSSFDGSEHQNRLSAVFIECPVCKQETPLGPKGIKALPCDYIVTNILDLSNLESEHIVCTSCKSKEEAISRCNDCANFLCNGCDNAHKYMRCFENHHVVRIEDLTKNVHDKLIIHKPVFCPQHVSENLKYYCFTCQVPTCNDCLLSDHKGSDHHYETATVAEQAVRADLEETLSDTLKKADYCNDAGSNLGSALTELQSQHDTVRQQIEDAYKCYKRMLESIKDQMLNELGRLHSDRELKIMDLMQSMENTMGKLQHAAQFGQRAVDKANSIEFLLLKPIISAQCLNLMEQTPKCDINYQLQFDSKPEMFEQFAREMFGKFQTDHIDSSPKKLGMTQQLQQQQQQQQQLQQQQHQHQHQVQQQQHHHQQQQQQKQQVQHQQQQQKLQLQQQQQHQHNSNNLIVGHRGSSSVQGRLTSAVFSPISLPSSLQSSFDGDTNSVMSNFSMMDSPPQPSSQQQLPSAQQQSQQQQQQQQQQQAVVLQQVQQQQKHQQHQVQQQQQQQVLHQPTSAQSMPQQLGQLVAPQGIVQQAGHVNLNAGPGPNISINGLGAGGPPPSFSMLEYNISRLASLTETMPDTLNDALAVGGGAAGPVPNVVAQVPGGSAVTGAAVVGASHAHQQQSQAVMPASAVTPTQPITLADILSGDQRALNNLQALAKLGLNNNDDGLLLNGSSTEIDFLSDFCMPPATSPSLQSLNPIVGGVEDMGLNNFHAVAAPGTTSVVTGRNKATPMQIRCKFGSLGTAKGQFNSPHGFCLGVDEEIIVADTNNHRIEVFDKMGALKFQFGVAGKEEGQLWYPRKVAVMHNNGKFVVCDRGNERSRMQIFSKCGHFMRKIAIRYIDIVAGLAVTAKGHIVAVDSVSPTVFVISEEGELVRWFDCSDYMREPSDIAIRDNDFYVCDFKGHCVAVFQDDGTFLYRIGNEKVTCFPNGIDISNAGDVLIGDSHGNRFHVACYSREGALQSEFECPHVKVSRCCGLKITSEGYVVTLAKNNHHVLVLNTLYVH