The following text describes a protein:
Function:
Serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis and regulatory volume increase in response to hyperosmotic stress. Wnk mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation. The membraneless compartment concentrates Wnk with its substrate Fray, promoting Wnk-dependent phosphorylation and activation of downstream kinase Fray. Following activation, Fray catalyzes phosphorylation of ion cotransporters Ncc69 and Irk1, regulating their activity. Phosphorylation of Na-K-Cl cotransporter Ncc69 promotes its activation and ion influx. Involved in circadian rhythms in small ventral lateral (sLNv) pacemaker neurons: in the morning, Wnk activity is repressed by high levels of intracellular chloride; in contrast Wnk activation in the evening promotes the activation of the inwardly rectifying potassium channel Irk1 via Fray. Acts as a positive regulator of the canonical Wnt signaling pathway during wing disk development.
Subcellular Localization:
Cytoplasm. Note=Mediates formation and localizes to cytoplasmic membraneless compartment in response to hyperosmotic stress.
Domain:
Disordered regions undergo liquid-liquid phase separation (LLPS) for the formation of a cytoplasmic membraneless compartment that concentrates Wnk with its downstream substrates.
Sequence:
MGQTLCDFVRQQEVVPANRARKGSAISEDGTTSCTTQPQRNTSISSEEQTVQGANIQKSGHRSFNRSASSRQKPNPTSKLKDTLNRKPTCKQGTLSAHTSTSSTTSIQSSPIEPASSLPTLNTTPTPPAASKSNLFVRVLRRFSNNTLPGKTASSPKNVDDVPKVNDNNNGDLGKQQSTDVEVLCSQDNQSREQNEDEMDSKIEPADAISNQKAGLTSGKPKKDKSVKGTSQAVVSDTKKMEARKSSSDTVIEPLIKQQAPLHSSKTTPTTLTANFVQNIRFVRKNVEQSGRNTNPLQFVELETEFPRDYDDNIEMLSREAEHLEEQFRTPTRSNATDATSHHVAGIIDNIITEASRSLTIEKTEDDVPLKSSTKHSSGVKRVGFQVEDKDDTEVQSEKQAKSFDDITKKAESSEASAEEAAVTGSSTDASASPLPSTSLVSTTSSATSITKSKSDEDDDPVAMSPCGRFFKYDKEVGRGSFKTVYRGLDTLTGVPVAWCELLDKQVKKSERTRFREEADMLKKLQHPNIVRFYTYWEFPIGRKKNIVLVTELMLSGTLKSYLKRFKKIHPKVLKSWCRQILKGLNFLHTRQFPIIHRDLKCDNIFITGTTGSVKIGDLGLATLKNRSHAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMAISEYPYSECKGPAQIYKKVISGIKPAALAKVEDPNVRDIIERCIELKKEDRPSCNELLESEFFDEDIGIRVEPTASEQFLSDPSISIIEFRLRFMDPKKRSSRHKENEAIQFEYNIRHDEYEQIAQEMMKENIISEDDSRAVARLLKVQVVSLLKERAQRQTQIKLQNEKSRLEKLALQKQRESLPTNVDEDEEEEEESEDEEDGVKWNQRLQLRYDLLNTDSETSLALSTNSVEPQQLSTRSNTSIPNSGIQQPVQVPGQVPVSQLISVQPQAIPSPAIPMQQKPTVHYIQPPQLASYQNSNASMQEMTNNQVISPTGSQQMQQQQPVVAPTVNHQVMPQQQVNQQQQQPQMMQQIPQQVQVQQPQTVLPPQPHEQQPQQQQQPLQQQLQQLMHTNVQAPDLTQQQQMAQQQAQQYFQQQQQQPQQAVNMQQAYAMQQAGQQQQLSQPLQIQQQILQQQQVAVSHQQQIMQQQLAQHQLQQLQQQQLQQQQLQQQQQIQQQQLQQQQLQQQQFVQQYAQAMPQQQHQQLVTGSQVMAPHQHQQPIQIPVQMQVPPTSVAPPIQHTYNQQGGQVTLSDAQQQQHPGFSAVPQQAAPFIQQPTQQPIQLSMPLEQQLQQLLHSQPAQQQQAMSQQQQQPLVQQQQLPLVQQQPPLVQQQQPLVQHQQPSVQHQQPLVQQPQQQQPQPQNQQPQPQTQQTHVVQQQPPQQQPAVEQISQISSQVPVQQENLQPIQVNKDANVATDAMSLNSAHGALEPAPKTEPQNSADAEKQQKQTGTGTRSQKPRRSNRSGNERIPKLSVTSVDEGSVINCHMENKLKTITFKFDIGDVNPVEIANKLIAQDLLSNCQSTVFVEMINEIVDQVKQNPNQIPIPTNYRRNIEKVRHASLTRQRSTFRSHQRHRSRDETASDITKMFEPTIHGVETLPSGGGGAEQSNCNLTLEARSHLSNIPNAKEPQLNVSTPPTTTSTMSSSSTASRDAPNSSNDVTIGSGSVSRKTSTASEYTSLSIDYMPDSNITPTGPEPPLDDGKDKKPCSLAIARMQKLLESAGNGAPRSLNLPLNRHLKIQEDLKHTRSLDDLTAVKITFDMPNKAALDTSENAQQATEAEAEKPKDKSGQAVGNQGTGAANTLEQLKIELENITHAHAFASAVVASINNRAPHQASPAMSSLKATSGQPQTQEITKPNNGAGPSVPSVGQNTPTAALTSARGSGSSVYNSRRTSIDNSVGSDMHLHTATNLEGTVSNPDPIPTEASVGITIATGHEKQLSKQPSLEKPSATSILTNNSDPPQRNPSNGSINQNSIADLEKKLAALRNTENTEESATATLSVVPKQVEEVVNPSARKISRFSVSRVQEQKTSTGVEEPAQGQLKIDLQVAGPGGQVQTNNSVQNGSVVNTPTEVISSPIQNVPLAINGIQLMYQQPQQIHQLPGGTSTTTSGAGTQCIVVPQVVNQNGTHVQQPSNLQPQQQSVHPNMTQQPQQTPLNGHPSMVNTLQQQPPQQSLPMQTIQSQQQQHNQMPIISQQQQQQILMQQQQQQGSQQGSQQFNLPGTQQTHPQHQFIQSQPNQLHSLPPQVVSMAQGNLPHQLPPMQTMGAQQQMISQQQHQLQMQSHMHQQNVPGMYNQQTGARVAAPQNFAGAVPNHLLQQSPLMASQQPAQPMQHVMQPIFNATSGEVTEEPVSLAATHPHLLPSDIQSDIKHNLDSLVNQLCNTRLGTNQHQRLLLLRQRQLIEEDELRLKHYVEYEKFQKALRQSISTNVPANAAYYSAAAAQLPTNLAAPAAPSSSNPTSTSSANT