The following text describes a protein:
Function:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide. Man(5)GlcNAc(2)-PP-dolichol is a substrate for ALG3, the following enzyme in the biosynthetic pathway; Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.
Subcellular Localization:
Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side.
Sequence:
MLFNSTQCMKTYIAEQMPPKKKPVAAGASGSKRRMNRESSSSESGESMDDSDYQLAMDIAQRRTTRQSARTCMLKIRGDSLVDEEMEAQEDQKPKDVSYDYQELLNDPAYNKPELYKSMEPEEFNMDYYHRTGLTEVLKFSCPPEKLGMKMVDDDFTVDDVEKLVGGDRMITVVVVATQQSEDMPLKKFIEFYKNVDGRQSNSSKDDTKRTLYNVLSLEFSNTALTDLVQSPSLVREIDWASSWPDERKMRSISFEDDGTFNIHNRYPRVENYCLMSPNECYTDFHIDFHGTSVWYHVKKGKKVFWIIEPTEQNLKMYEEYMKNTESSAFFGTIVETCARVEVLPGNTLIIPSGWIHAVYTPVDSLVFGGNFLHSRSALMQLNVLQGENRIGINKKYRYPYSEEAVFFYLNKVVKEVTGRQHIRPMSRNQQNHYYEYVGEQFREKNMHHRVPLKADYDDDGVTWKQRWEETAEYIESKAKLAKWDGNGMEEMEKDEENDVKKVDGDYDGMPILSPKKEVNDGMPVLSPMKEVNEDEKTIDVKVEEKDMPEFGGHDDMFIDDLTFYHPVSFNLDAGRGATAVHTLPLSTVEPRIKISKVTLKKAIHQLEVVQWEGLIVNLLKKSKVDVPDGLTRPNSLIQTFCKLLKWRRKIVEEEEGIKLGRCVLPDKEGSRRLHEEQKRAAAERKRKNEEKKMKIEDGDGEEDIDWTEDCEQPKKKKTRKSEEVGKMGRWMTMTDVSTRDYRPPKEKDYKVKWEEDAVRFHTAARRKLYWKNERYKLEVKHDHLLDDDDHDDTHRDEIDVVDGGITHSMITRGGEMMEGINVHTGGDGPSHPITSTSASNDHSSPLNIQSRSSSFSRRPIQTMQQTPSLILYASSPIARRISRISTDGWGGMNGERRDRQSSGRSPTRKPSSQFSSSDIPPLSLTPSHTPHTPVTPTTSSTPIHSTTLSTSKQTRLSQAEQFTMAAPKVIDAPRLATPLVPTQSSNSDLSVLSQSNSISGIDPLPSDIWCGQMERVHAFSSTKMIYWLFASILTLLFLSYRRRKPNTIAFFHPYCNAGGGGERVLWCAIRSMQARFPKLKYVIFSGDRDASKEQILLRAKQRFDIEIDPSNVDFIFLKLRTVVEARWYPRFTLLLQTLMGGVLSLEALCYFCPSLMIDSMGYPLSLPIFKWFGGCHVAAYVHYPTISCVLTVCVYNVDSIAYMIDLVARRHTSYNNNESIAHNATLSLLKLYYYKTFALAYGLAGIAADVVMVNGSWTASHISKLWKQTCSIVFPPCDIEGLLALPGTSEEILTNEKRVNILSIGQIRPEKDHRLQLEVLKEVLEGIKKKKLDVKVSLTIAGGCRNEEDEMRVNHLKEYAKKLGVEKNLEWKLNVPYGELYQLLNSSLISIHTMRNEHFGISVVEALAAGTIMVAHKSGGPLMDIVDPCEGVASVGYLADTKEGIQYTRFASRPRNFVPEMKVFALQVLKKSDDGKGTILKTANNLASFSFFQRGSVQCKMLMIEEFMTFTGKLLVERTAAGSRSTVKENEYYCHVHVRPDGLAGVTITDGEYQSRVAFSMLTRVLDDFNQKTPNWRSINDEKACGYLGLPEFLERYQNPREADPMTRVQEEVEETKIVMHNTIQSVLERGEKLDDLVKKSENLSDQSKMFYTQARKMNKCCNYV