The following text describes a protein:
Function:
Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of pyranonigrins, a family of antioxidative compounds. The first step of pyranonigrins biosynthesis is performed by the hybrid PKS-NRPS synthetase that condenses 6 malonyl-CoA units to an acetyl starter unit, to form a 1,3,5-trioxotetradecane-6,8-dienyl-ACP. The enoyl reductase (ER) domain of pynA is likely to be functional during the first two rounds of polyketide chain extension, to generate the saturated C-C bonds of the alkyl side chain (Probable). PynA subsequently forms the amide bond between the acyl chain and L-serine. Although pynA has a terminal reductase domain, it appears to require the thioesterase pynI for the release of the straight-chain intermediate from pynA via the formation of a tetramic acid pyranonigrin J. The methyltransferase pynC then coverts pyranonigrin J to pyranonigrin I via N-methylation. The FAD-dependent monooxygenase pynG catalyzes an epoxidation-mediated cyclization to form the dihydro-gamma-pyrone moiety, followed by pynD-catalyzed oxidation of the alcohol to the ketone and enolization to yield the characteristic tetramic acid-fused gamma-pyrone core of pyranonigrin H. Pyranonigrin H is substrate of pynH for dehydration-mediated exo-methylene formation from the serine side chain to produce pyranonigrin E, before the oxidase pynE reduces the exo-methylene of pyranonigrin E into a pendant methyl to form pyranonigrin G. The FAD-linked oxidoreductase pynB performs the reverse reaction and converts pyranonigrin G back to pyranonigrin E.
Domain:
The N-terminal part acts as a polyketide synthase and includes a ketosynthase (KS) domain that catalyzes repeated decarboxylative condensation to elongate the polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) domain that selects and transfers the extender unit malonyl-CoA; a dehydratase (DH) domain that reduces hydroxyl groups to enoyl groups; an enoylreductase (ER) domain that reduces enoyl groups to alkyl group; a ketoreductase (KR) domain that catalyzes beta-ketoreduction steps; and an acyl-carrier protein (ACP) that serves as the tether of the growing and completed polyketide via its phosphopantetheinyl arm; The C-terminal part acts as an NRP synthetase composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module (Probable). Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product (Probable). PynA contains one module and terminates in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme (Probable).
Sequence:
MDTPLSSSEISPRFSNTVPSSVSSMTPNADPSVIVGLACRVPGATNPSQLWENIVAQKDLQRKMPADRFNVDAFYHPDGTNKGTTNAKFGYFLDQDIGMFDAGFFRISGKEAEAMDPQQRLLLEVVYEALEDAGITLDEVNGSNTAVFCGSFTNDYNAMVTKDLEYYPKYTVTGTGNAILSNRISYFYNLHGPSVTIDTACSSSLNESDIAIVVGSALHFDPNVFITMTDLGMLSSDGRCRTFDSMGSGYVRGEGICAAVLKRRRDAVYNGDNIRAVVRASGVNHDGIKQGITLPNTNAQEKLIRRTYDLAGLDPNDTQYFEAHGTGTARGDPIEARAIGAVFGSTRSEPLYVGSVKSNIGHLEGASGLAGIIKATLALEESQIPPNMHFKRPNPEIKFDEWKIQVPQDIISWPASANGIRRASINSFGYGGTNAHVILDAYKPEDSEAELQAIPAISSSIPVDRPYLIPLSAHSTKAGALWEDKLTKYLSNEPIGRPAVSDLAVSLSTRRTMHGNRSFIIGKDMPTVLQGLEQPPSPAAAWTRPLKETPRLGFVFTGQGAQWFAMGRKLIQQSHLYRQTLERCDAVLQSLPDGPDWTVLEELLRTEEASRLKETRLSQPICTAMQLATVCLLKQWGIEPSAVVGHSSGEVAAAYAAGILTFENAMIAAYYRGLYMSSGVDGSMTTDGAMMAVGLTEAEAKKEFETYTGQICVAAVNSASSLTLSGDKDAIVRLRDSLVERKIFARLLQVAQAFHSHHMLPLAPKYEEALKNCAGFGTSPARVRMFSSVTARLARPGEMGAGYWTANMTGTVRFSDALTGILLNEEDEQNVDILVEIGPHPALKGPSRQVMNALKLNLPYLASLTRGVNDYESLLTLAGQLFQYGFPVDLIAVNSDHFLQRETGMIQSELHGKRLRDLPTYAWDHKRYWSETRPIREHRLRKQRHSILGARMPGIPERTPHWRNYLRLKEIPWLADHVIDGNAVFPAAGYFSMAIEAAVSMCAEDSVIKEIALRDLNVQSALLLSDSEEGTEVIMELRPATQSAKSKSALWYEFTIYSYGESKILNEHCSGLVSVETNALTLPMRWESSKTFDDLAKESQESIPAETLYDHLTALGLQYGPSFQLLTGDVQTGPGFALAGLDFQPSQFSVQAADLTIAHPTLLDASFHAIFPAIESALGRSLDEPLVPTFVRSFKVSGDFLACCRESREQKFQVTCFTRLPGPRVALSDLTVCSKESNKPLLQFNGLEVTALGSDKTDNSAGRSLFFRTRWQPAFTFLGPDHPAVAQKNISEILDIFAHQFPDTRILHVSDTVDGTRDVLKYLGGRSNERRRFHSITPVFQTQIALEEIDALSQEWPGLVEISEPEPNAYGLVVLSSDAADLDSRQFVKEGGFVLALGPHPQPEGLHDVFFTKDLAVWQKSTDNAQKPKQLSLILPSCPSQRTLDIADGMEMQHGSSVFVTRTSLAALSNEALQAEDIVVLANLDEDVLFEHSSSDQSTFLGIKRLLTAGGKNIVWVLEGGSMDAPKPEHAMIIGLARVARSENDQLRFVTLDLPRASTQETVVRHVWRLLDRSITEDEVAVRDNCIFIPRVEADDQLNSKLRNGTNSQPREEPLGAGRPLALKIGRVGLLETLVFEDDEQILDTQLADDEIEIEVKASAINFRDIAASMGIIDDYKLGDECAGIVTRIGAQVNPQDFQVGDRVAAWRPGQGAHKTIVRNPASLSYKLGDMSFVDAASLPCILTTAYYSLVHVAHLQPGETVLIHSAAGGVGQMAIQVAQYVGARVIATVGSQAKRSLLKSRYGLADDMIFNSRDDSFVRDVLDTTGGRGVDVILNSLAGKLLHATWSCVAPFGRFIEIGKRDIHENSKIDMDPFRRNVAFASVDLVTIFEKNKPLGARLLKECGTLVHKGHITPPETVTELPYSDAVKAFRLLQMGKHTGKVVLVPHAGDRVPVLPSTYRNQPLFKHEKTYLLVGGLGGLGRTLAEWMVRKNARRLAFLSRSGADKEEAKRTVEWLRERGVSVTVFKGDVSRYEDVERAVKAIDNLGGIFQAAMVLQDAPLENMSYQQWQICVEPKVKGTYNLHQATLGKQLDFFICFSSASGSIGSKGQANYSSANCYLDALMRHRREMGLAGTTMNCGMIVGIGAVAANQALLKVMMRSGYDGVNKEELLYQIEEAVLSDNNKKVSRRGVDLHQTITGINMTKADFYWCQKPLYRNLYNNHEFLGQTAIKQGTKSLASQLQGTKSVEERTTLVLSAFIEKVADVLSVSVDSIESANPLSAYGLDSIIAVEFRKWFSRSVGVEIALFDVLGAPSIFALVTKASGLITITTSNDDKAENVDNEGAKGNEDQEVETQQGQLNQPIPPAAAVGPVPMSSFQQRLWFIHNFGDDKTFLNLSITSYLEGNPDATILEKALNELVNRNAILRTGYTEGDEFAEQTVLDMPSISLERIDVSSKPSPTVSLQDVIQRRRAIELEIEEGEVVRPMLVRLSDDQHALVLICHHIAIDRGSAKSSLNQLTGIYDAIRQGRDLDMVPRPGVSYADFAVWHNRLLSSPSLQADLTFWKENLSGMPKTCKLLPFAKSERPLHDDLQRTVVSGILKKSLLNRMKRICSQSGATPFQFLLAAFRAFIFRYTEDSDLGILMIDGDRPHPDLEDVLGFFVNMTPIRCQDSCEGAFDQLLEATKTRTLEAMSHSKAPFDSIVDVVKAKKTTSHFPLAQIALNYQIHGTFPVYRTQDFNVHDVQSVDVPTACDMQLEALEHPERGLDLRLEYSSTLYGSGDMNRFFDNFVTFMSSLIRDHRQPIAEVNLCGALEIAHLEKNFWNTQFTQNPWGSVGVCQRIMENAAKQPEAVAIAASDGAAITYSELVERAQRVAASLKASGVTERQKICVLVDPGVDAVIALLAVLLTRSCYVALDSSFAVDRLAFMASDCGAGVLLFGPELQGLAETVASKSKSGLRLLDTKKAALCEDRFVGDLPSVNEDPFFIIYTSGSTGKPKGVVLSHANTQQMLASVGEYFRFTSDDRFLQQSSLCFDLSVVQIFSALTAGARVCVAKHDIRKDPAALAAFMHETGVTITYFTPTHFALLLEHSWETLHQCSQYRAALFAGERLPVRIARAFYDLQTPAVVYNTWSPSELVVQTTIHKVDKPDDDVFDIPIGRPLPNCRHYVVDAVLNPLPAGFVGEICVGGAQVGLEYLNRPLANATSFVRDLNSTPEDQARGWKKMFRTGDKGSFLPNGLLTFKGRIAGDKQIKLRGFRIDLGEVEQVLYKNASTPDGQGIVDISVIARDSEKSDATSPLTDERRLIAFVIPKKPLQSTQERDEYANYLHRMAQGSLNEYMCPNGYQFLERLPMTIGGKVDRRSLLTMKLDLAQHTTTCTDSRPVTEVAGDDAEILQGVTGQVCSLLGIDRSIAPNDNFFELGGQSILLLRLQSRLKKKFKVTLKLQELIHAPTPLAIAGMIQKQLKGPAGVQNENASKSIDWSEEISLPASLMNTDYSQLSRFPRTDVSSILLTGIDTFIGLHMLATILSNNHNATVYVIGIHDELTADHLVEGLTKYKLLDAHLSTEDVLSRTCAVPGKMTSPRFGLAEEAFRNLADKVRVIFNIAADVSLLKTYVDLKTVNTSAILTLIELATSSHGHLLEIHHLSTWSVPHLQTWKKTSRTRAFASNREEDPSHFTPPTADEYGYFKSRWAAEMYLTQAAARGVPVSIYRASSVSGSRATNVPVPEMDFISNMIMHMIQHRAIPEINSSSLIDEAGDFVVDFLPVDALTSSMYTLASEESAAAPGLQVYHLGSSQPLPLQALVDVIPSLDQSGAAGKCRVVPMQEWLRLVSEGASEEEQLHWMVVKKYFQHGHSMFALDKSHTVAALKKAGKEVEFPAIDVDYLKRLLDERGPGLKR