The following text describes a protein:
Function:
Displays RNA-directed RNA polymerase, deubiquitinating and deISGylase activities. RNA-dependent RNA polymerase is responsible for replication and transcription of the viral RNA genome (By similarity). During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription (By similarity). The deubiquitinating and deISGylating activities specifically cleaves poly-ubiquitinated conjugates and ISG15 from RIG-I, interfering with antiviral signaling pathways mediated by NF-kappaB and IRF signalings (By similarity). Favors K48 poly-Ub linkages.
Miscellaneous:
MISCELLANEOUS: Classified as His(+) endonuclease since it has a histidine upstream of the active site that coordinates the first cation.
Domain:
The viral OTU domain (vOTU) and its N-terminal extension is responsible for ubiquitin and ISG15 binding and for the deubiquitination and deISGylation activities.
Sequence:
MDFLDSLIWERVVDEQYITNPTFCVSDYFEVIRQPGDGNCFYHSIAELFFDVKTPSSFRKVKEHLQLAAEVYYDTEPEAVGTGISKDEYIKVAMKDNEWGGSLEASMLSKHLQTTIILWVVNSTEQVTAAIKFGPGRVSTALNLMHVGRTHFDALRIIEQLENNQPQDRNRLDIADRIAAAEVYVRQSIEDNLQEDEFFDYAREDEISEDVSAPGGSREATELKKKAILLNKTVKRGENIPIRVGRVLDCLFSCKIAVSLDEGLLYLRPETRESEATSISLRQLGHKLLTRDRHIKMEYARSKLYVTRDLIDHLDIGGLLRSSFPGLGLERYIQLLHSELVLDLVTVVLAVLLSTFLYGSNNKNKKQFITNCLLNTKLSGKRVFKALSKLTGQMLYRTPKRAVSIVSQELYGKLMLKVKNNLEGMGPISMLALRNLNFDNMQLQDYLEMLSEMSKIDNSDVEYTHREISDLHTLVERLSKLQKSQDVNELKLWFKEEVLTKRSQRSVGNAFEFLINDYFKKKDIMKFVSTSGKASSTGNIGNVLSYAHNLYLSKESLRMTSEDVTQLLIEIRKLHKLQGDLSIEPVAIICDKLEDQFRKLFRELPEECSSECQTLFNDIRNSPSHSVAWKHALRLKGTAYEGMFAKQYGWSYISEDIKPSLTMIVQTLFPESFEAFLDRTQLHPEFRDLTPDYALTQKIFFPRNTIPRTENRQLAIDVSLEGSVEAVPIVEKRMFPLPEVPIGEANSISRVMNIFKEKREESMQKKLEHDRQAEANRLKSAGLSASKAEQEVCNSAQDRKEEKERTTEPAGKQQRTEDLVVIEGNQDEGDSDPQKKVDEKTVPGESKQHSKSSGSSSTNQMSQKVVDVPSVEDSSDQAPGDFPDYGYYFKRIVMDESGTVLTEEAQLEKRQLLFIEVGYQTDVDGKITTDYKKWKDILRLLELLNIKCSFIACADCSSTPSNNWWISEDKVRLLKNSISHLFSKLTQNSPADVTDIVVGSISTQKVRSYLKSGTATKTPISLKDVQETWSKMKDYIVNRPTGISLNKELVGALYQGLVEGAIISKEGTVNLIQMLKDKQERITDEFERTKLKHEVNEDVKTSEKLLLGWLMEDLKGCRCMGCLTKIKELSESMSVNQDRLEYLSTNCQTKSHCTECHPRSLEYRNISNVDNRVPSMQRVSHSRNEGFEDTNETLTELDRLVRLTLPGKTEKERRVKRNVEGLIRFMMQQSSLDCIKLPSGQIIAHRCSKKFKNSSEAEEKCNERFERLMKELSEQKLKPYSDHVRKTITSSLKKTDKQAGSKCAVPRLWLETLIRDLRVPTKDEEILLNIRTSMQSKTNFIRNNDKLIIRSNKEIADYLETKRKNLLSEKASDKIFSSDCILFKEVIAEALRRYYSTPYEGVPETIVKLINFLCTFGWFQEVVLYSKICETFLRCCTEFSRSGIKLVKVRHCDTNLSIKLPSNKKENMLCCLYDKNMSLLKGPFFLNRRQAILGSAYPYILITLYIQVLQQHRCLEVLNSVNDRVVGNINTCTSNLLNTVKAELTLVNSGLFEKAYECRTEQCRLGGNFLNRSSRDHFISTVSGLNVVYGALIKDNLLANSQPQNKQLQMLRFGMLCGLSRLSSALELGKKFSTSCRRIEDNIMRLYLQSTIYSANRDVSQNVQNWKMKDLCPDITIPCFSVYGLFVNSDRQRIFDIYNVHIYNKEMDNFDEGCITVLEETAERHMLWELDLLRSLEGDTRDVRAARLLLGCPNIRKATDKDGNRLMKKGITDDWREEAGSDSSSISGRRSYASSGTRVKSMFGKYNSSQKPFELKPGLEVVNDPLHDYKQAVQDSFCYSEYTPNTESVLKDCIHIIRTNPSHTMGSYELIQAVTENARRKYPPENIERARKDPKNWVSISEVTETTSIVSQPRTHFMLKDCYKVLLGTENKKIVKMLRGKLKKLGAMRTDIEIGKKDCLDLLTTVDGLSEEQCKNIVNGIFEPSKLSFYHWKDLLKKELSEVLLTDDGNYIYCWLKTLSSMVKHSLKKDLRFMTGKNSLDIKPEMFTDEEYSALNIMKLELLGEHTDGIQGKTDFLLSSWKKCALKPKEGQSILNVGLNSLAALHDELYDIRLQHLELTRIKKENPTVSFTKEEILVKRLEKGFLNKYKKEVMEAVNLIFYCCLTAPWCLHYKSLEAYLVRHPEILETECIKENDIPLLDLTVTSLIRSLIDDIEGESSFNDSSDIKVRFAVKYLITLFTANGEPFSLSLNDGGLNDDLQLTTDEKLLYQTKKVFAKLGLSGNNYDFIWTLQMIANSNFNVCKRLTGRTTGERLPRSVRSKVIYEMVKLVGETGMAILQQLAFSQALNYDHRFYAVLAPKAQLGGSRDLLVQETGTKVIHATTEMFSRNLLKTTSDDGLTNPHLKETRLNIGLDMLSTARALDGKQVSDDSNLLNFFKTVCISGDNTKWGPIHCCSFFSGMMQQLLKDVQDWSSFYKLTFIKNLCRQIEIPAPSIRKILNVLRFKLSDKGGVEKLSEEAIRSELINNLAEWEGNDTVKFLITTYISKGIMAMNSYNHMGQGIHHATSSLLTSMMAETFEELAVDYMKKHFPGLTVNVDHAGSSDDYAKCIIVSGLVSKDMYKRYDGVFWRHMCRLKNFLAAVRRCCQMKDSAKTLVGDCFLEFYSEFMMGNRVTPAVIKFIFTGLINSSVTSPQSLVQACHVSSQQGMYNSVPLVTNAAFTILRQQIFYNHVEDFIRRYGLITLGAVSPFGRLFLPRFSGLVSSSVALEDSETISKAAAEINSNDIFFNTSSLSNLDKLEQSPDSSGLDDDSVVSTTTVESSDSKGSSSSFTFDLNRPLSETEVKFLKLLRELTSTTACEMLQEKINTLYNDSREGPLDRHNILQNCRLSESCDWLLDGKKRGLLELSRRMSCLLNVLIAGYYRSFGSEGTEKQVKASWSRDDNRVIEDPMIQLIPEKLRRELERLGLSRMEVDELMPAVGPDESLSQLVAKKLISLNVSTEEYSAEVSRLKQTLTARNVLHGLAGGIKELSLPIYTIFMKPYFFKDNVFLDLEDRWSSRHSTNYRDSTGKMLTGKVVTKFTHWLDTFLSCVVSANRSQEIKECSLFNPNLRCVNIMVKGNGIKELSYIRSHLSVLSVEFENLNLQFSDVNRQKLKIVESRPPECELEANKAVIIKSKLFSAVEHVRLSNNPAVVMGYLLEESSISEVKPTKVDFSNLLKHRFKLMQFFPSVFTLLRSLQSESKELEKLGEPVDMHQVSKYANHLTLLCRMIQQSKPSLTVFYMLKGSQMNTEPTVSELVSYGIKEGRFLKLPEIGLDASTYSVRYWKILHCISAIGELPLSSEDKTSLLISFLNWKVTSDCVDDCCPLEKYDKAIVSEFSGQVLINTLASELSSVRKDQEREGLTDLIDYINSPSELLKKKPYLGTTCRFQCWGEGAKSGKFTYSSRTGEAIGIFVAGKLHIHLTSDSPGLLCEVERQVLSWLGKRRTDVLTKEQHQFFLDFLPNLSEVVQKNRDGAILGVTIDSTNVRMLKYVPPKRNTPVIKIKKQILTVKKQTTLDVESEPRIVWGHGQLSIVYDECETETTYHENLIKVKKLVDLASGTTDKLPTAIFSDTRITLARVKFKTELLLNSLCLLHCFLKHTSQDAIQEVESKCNVLERYLRSGGVQFRPMSESLDKKVTKLPLQCQSDKDVDKEINFCEDLTRVFSNENVPLSSWSEVQSYIEEVGFGNVLVHIEKNPTRSDLIWRFSIDSISGNFGPIKDIRTLVTYMSTETVPKFLLPFLLFEEQLKHLIAGCVELRDALNSSGINDREIAIVALFTCFYYQSDSVKRQGPVCSISSFCSLIGDDLLPLDNRLQARVLPEQDNVKLHFKLNLTTDSALGKKDKAIQAKKIISRYLRLIFTEDDMDLKRLKSDATKVKLSSEKECEFLEFCLHSDLSYALNYRVLLEHLIDLEDRAKKTACVLIEEFILMLTGRLMISSTIDSDSKRTLEDDALCLEDLLDSDNEASSSKSDNEEQIALQTGKFNFNWDSD