The following text describes a protein:
Function:
Methyltransferase: Displays a capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m(7)GTP or m(7)GDP. GDP is a better substrate than GTP. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m(7)GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure; Y-domain: Indispensable for virus replication; Putative protease: The putative protease domain although necessary for replication of the virus may not be a protease but rather a structural Zn(2+)-binding domain. Inhibits induction of IFN-beta by MDA5 and RIG-I pathways and down-regulates the expression of MDA5; NTPase/helicase: Multi-functional protein that exhibits NTPase and RNA unwinding activities (By similarity). Hydrolyzes all NTPs efficiently and unwinds RNA duplexes containing 5' overhangs (By similarity). Possesses a sequence independent RNA-5'-triphosphatase (RTPase) activity suggestive of its role in forming viral cap structure. Also participates in viral genome replication, RNA translocation and genome packaging/unpackaging (By similarity); RNA-directed RNA polymerase: Plays an essential role in the virus replication (By similarity). Binds to the 3'-end of the genomic RNA to initiate viral replication.
Subcellular Localization:
Host cytoplasm. Host cytoplasm, host perinuclear region.
Domain:
Contains a methyltransferase domain, a Y-domain, a putative protease region, a zinc-binding region with similarity to calycins, a proline-rich disordered hypervariable region (HVR), a macro domain (also called X-domain), a helicase domain and an RNA-dependent RNA polymerase domain (By similarity). Since the boundaries and the activity of the putative protease are not clearly defined, the zinc-binding region might be part of the putative protease (By similarity).
Sequence:
MEAHQFLKAPGITTAVEQAALATANSALANAVVVRPFLSHQQIEILINLMQPRQLVFRPEVFWNQPIQRVIHNELELYCRARSGRCLEIGAHPRSINDNPNVVHRCFLRPVGRDVQRWYTAPTRGPAANCRRSALRGLPAADRTYCFDGFSGCSCPAETGIALYSLHDMSPSDVAEAMFRHGMTRLYAALHLPPEVLLPPGTYRTASYLLIHDGRRVVVTYEGDTSAGYNHDVSNLRSWIRTTKVTGDHPLVIERVRAIGCHFVLLLTAAPEPSPMPYVPYPRSTEVYVRSIFGPGGTPSLFPTSCSTKSTFHAVPAHIWDRLMLFGATLDDQAFCCSRLMTYLRGISYKVTVGTLVANEGRNASEDALTAVITAAYLTICHQRYLRTQAISKGIRRLEREHDQKFITRLYSWLFEKSGRDYIPGRQLEFYAQCRRWLSAGFHLDPRVLVFDESAPCHCRTVIRKALSKFCCFMKWLGQECTCFLQPAEGVVGDQGHDNESYEGSDVDPAESAISDISGSYVVPGTALQPLYQALDLPDEIVARACRLTATVKVSQVDGRIDCETLLGNKTFRTSFVDGAVLETNGPERHNLSFDASQSTMAAGPFSLTYAASAAGLEVRYVGAGLDHRAIFAPGVSPRSNPGEVTAFCSALYRFNREAQRHSLTGNLWFHPEGLIGLFAPFSPGHVWESAKPFCGESTLYTRTWSEVDAVSSPTRPDLGFMSEPPIPSRAATPTLAAPLPPLAPDPSPPSSAPALDEPASAATSGVPAITHQTARHRRLLFTYPDGSKVFAGSLFESTCTWLVNASNVDHCPGGGLCHAFYQRYPASFDAACFVMRDGAAAYTLTPRPIIHRVAPDYRLEHNPKRLEAAYRETCSRLGTAAYPLLGTGIYQVPIGPSFDAWERNHRPGDELYLPELAARWFEANRPTRPTLTITEDAARTANLAIELDSATDVGRACAGCRVTPGVVQYQFTAGVPGSGKSRSITRADVDVVVVPTRELRNAWRRRGFAAFTPHTAARVTDGRRVVIDEAPSLPPHLLLLHMQRAATVHLLGDPNQIPAIDFEHPGLVPAIRPDLAPTSWWHVTHRCPADVCELIRGAYPMIQTTSRVLRSLFWGEPAVGQKLVFTQAAKPANPGSVTVHDSQGATYTYTTIIATADARGLIQSSRAHAIVALTRHTEKWVIIDAPGLLREVGISDAIVNNFFLAGGEIGHQRPSVIPRGNPDANVDTLAAFPPSCQISAFHQLAEELGHRPAPVAAVLPPCPELEQGLLYLPQELTTCDSVVTFELTDIVHCRMAAPSQRKAVVSTLVGRYGRRTKLYNASHSDVRDSLARFIPAIGPVQVTTCELYELVEAMVEKGQDGSAVLELDLCNRDVSRITFFQKDCNKFTTGETIAHGKVGQGISAWSKTFCALFGPWFRAIEKAILALLPQGVFYGDAFDDTVFSAAVAAAKASMVFENDFSEFDSTQNNFSLGLECAIMEECGMPQGLIRLYHLIRSAWILQAPKESLLGFWKKHSGEPGTLLWNTVWNMAVITHCYDFRDLQVAAFKGDDSIVLCSEYRQSPGAAVLIAGCGLKLKVDFRPMRLYAGVVVAPGLGALPDVVRFAGRLTEKNWGPGPERADELRIAVSDFLRKLTNVAQMCVDVVSRVYGVSPGLVHNLIGMLQAVADGKAHFTESVKPVLDLTNSILCRVE