The following text describes a protein:
Function:
Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of aspernidine A, a prenylated isoindolinone. The starting point of the biosynthesis of aspernidin A is the production of orsellinaldehyde by the non-reducing polyketide synthase pkfA, via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units. Orsellinaldehyde can be converted to diorsellinaldehyde and triorsellinaldehyde spontaneously or via as yet undetermined enzymes possibly unrelated to the pkf biosynthetic gene cluster. Hydroxylation of orsellinaldehyde, methylation of one of the phenol groups, and prenylation, presumably catalyzed by the prenyltransferase pkfE, would be needed to yield aspernidine D (Probable). Subsequently, the cytochrome P450 monooxygenase pkfB is responsible for hydroxylation of aspernidine D to yield aspernidine E. The dehydrogenase pkfF may be responsible for further oxidation of aspernidine E to form a dialdehyde intermediate which is further transformed in a series of steps, some of which are enzyme-mediated, to generate aspernidine A (Probable). The possibility that additional enzymes outside of the cluster are involved in aspernidine A biosynthesis cannot be excluded (Probable).
Domain:
Multidomain protein; including an N-terminal starter unit:ACP transacylase (SAT) domain, a beta-ketoacyl synthase (KS) domain, a malonyl-CoA:ACP transacylase (MAT) domain, a product template domain, a acyl carrier protein (ACP) domain, a methyltransferase domain and a reductive NADPH-binding domain that is required for NADPH-dependent product release.
Sequence:
MQGSSHTLLAFGPQSLSFDKRALIKLRGDISSGRYGQWIQDTIAGLPGFWTALSEALPELRELLPPQERLQDLDRLLNHSIDDIEEGLSISNMILTPLVVLTHLTEYERYLATNQHTSDRTTATGFCTGLLSTFAIASSRNDQELAKYGAVALRLAMLVGALVDAEERRQELQGNGRSVSYSIAWMSEEQQQRLQEILGQRHPETYLSVRYDRARATLTSSGRAAADLAQELREIGLTVAQVRLQGRFHDPSESRKAYTDALADLCNRTPELQFAPTPAMSLAYPKIDNASLHEVAVRSMLVQHCDWYDTFKAAYPEVSSPARVVCFGSERCIPPTLAKRTVKIESQHQASDAFASLDQENMIAVVGMSIKTAGADDVDEFAQMLRTGTSQHQAIMEDDINFNTPWRTDKGSRTWYSNLIRDRSQFDHSFFKMSPREAAAMDPQHRLFLQAAYQAVEQSGYFLEPGHNETKLPEDSVGVFHVSCYDPNAYTATGNLRAFLAGRVSHHFGWTGPALTLDTACSSSAVAIHLACRSILSGECSAALAGGVAVMSSPFWFQNLGAASFLSPTGQCKPFDEKGDGYCRSEGIGCVMLKKMSAAVANNDQILGCIGASAVHQNQNCTAVVVPNKPSLAPLFKQVISKSGLTPADISLVEAHGTGTAVGDPAEYASIADALNVASRPSPLILGSVKGHIGHTEAAAGVVSLIKVLMMMNEGFIPPQASHTRLSPRITAATDKLRISTSLRPWETDQKAALVNSYGASGSNTSMVILQLPKRHTFPHQGVTKDGLAQPFWISGTNEVEITAYCARLAEYIRTRPTVRLDDLSFNLSRQCNRTLARQLVFSCRSMSELQAMLSRGASAEGSSKVVPTERPVILCFGGQVSTFVGLNRLVYENVGLLRRYLDECDMAMTNMGLESIYPEIFSTSPVQDTVKLQCMLFAMQYSCARCWLDCGVSTRIAAVVGHSFGELTALSISGVLSVQDTIKLVAGRARLIRDQWGSDRGAMMAVEGDESTIQQLLEEASQQTPSDHPATIACYNGPRSFTLGGSTAAIEGVKETLLSKYPYIRHKRLNVTNAFHCSLVGNIVEPLNQLGEELTFNSPVIHIEHSSSTPSTGARPSSTFVADHLRKPVYFYHAIERLARAYPDSIWLEAGSNSTITTMAGRALRPTSDIVGQSHHHFQALNITHGGRGSDSLTETTLSLWKEGLRVSFWQHHPVQKDQYEHLILPPRQFARTRHGLDIIAPKTDQGAAQQTGVPKPDGLWTFVGYEDNNRSRPRFLINSNSEKYLAMVEGHVMARTAAICPAGLEVDMAIEALFSLQPEWKERRLQPMLRDMVNSAPVCLDPIRKFWLQLSAADESCMLWDWNITSTREGSSSSLLHVKGSLQIRSPEDPLYRNDFARLERLVPYRQCTDILALAGAQPDDLDDEIHVLQGRNVYQGFSDVISYTPLYRSLRRLVGRGNESAGCVIRKRSHETWLDVPLSECFMQTGGIWVSCMTDRSPDDVFIATGCELWMRSPSLADVKEKGEDGTEKWHVLARHLRVSDKVYLTDVFVFDSRTGALTEVIMGTKFSRLPKTVMRKILSDFHPGNPSVHEKDIVTAAPKQPIIQAQEIVLAEAPRKHKSVQKRDITGQVCQLLSSVSGIEPAKINQNAELSDLGVDSLMAMELAREIEGVFKFSPDHNDLLEATTVEKLTNYISSSIPGSSAASENIDNHGSPNDTDSSGESDPATPTVACSSTDDFREPFIITSSRDEAEVLSLAESDDTFLKSQRPIVEMAKQNTDREEIAEQYVARYTAGFIPPVSSRSSAREHSGNVVIITGATGSLGSHLVASFARDPSVRTVICLNRRSKTPGTLRQSQALSTRKLQLTEAESSKLRMLDGNTSEPHLGLAADEYTWLARNATHIVHNAWPMSLARPIHTFVPQFQALRNLLDLAREMACNCSPNGMRVGFQFVSSISVVGSAAQQRVLEQRVDLRSSLPIGYPEAKWVCERMLDETLHRYPDHFRPMVVRPGQITGSSHTGCWPHTEHMPLLIKTAQTLQAWPDLDGPLHWVPVDTVAQTMVDLLLPGIESDSNQTGTTLNKSEPDAYPVYHIDNPVGQPWKEMTPIITSALNIPSDRVIPYQEFLNLLRSSPLDRDREIPAAKIMDFFTRDFEHMACGGLVLDTTLSQEHSATLAAQGPVTEEENMKILTL