The following text describes a protein:
Function:
Catalytic component of a deubiquitinase complex consisting of bam and otu. The complex deubiquitinates K63-linked polyubiquitinated proteins; this antagonizes the ubiquitination activity of Traf6 and regulates the IMD immune signaling pathway. Otu-bam deubiquitinase activity is regulated by Traf6 dependent immune signaling regulation of bam expression levels; this forms a feedback loop that regulates the IMD immune signaling pathway and balances gut immune activity during aging. The complex deubiquitinates and stabilizes CycA/cyclin-A to regulate CycA-dependent differentiation. Involved in grk mRNA localization to the dorsal anterior region of the oocyte required for dorsal-ventral axis determination; may function as a ribonuclear protein complex together with sqd and Hrb27C. May regulate actin cytoskeleton organization in differentiating cystocytes during fusome maturation; required for efficient nurse cell cytoplasmic dumping during oogenesis. Essential for female fertility; involved in germ cell proliferation and germ cell differentiation; [Isoform B]: Involved in the early stages of germ cell proliferation and differentiation during oogenesis. Required for polytene chromosome dispersal in nurse cells during oogenesis; [Isoform A]: Involved in the later stages of germ cell proliferation and differentiation during oogenesis.
Subcellular Localization:
Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, perinuclear region. Note=There is contradictory evidence of what happens to otu localization in nurse cells during vitellogenesis; either it relocalizes to the region of the cell cortex in close apposition to surrounding follicle cells or it takes on a granular perinuclear localization pattern (or both occur and can only be visualized under certain experimental conditions). Forms stable amyloid-like granules in the cytoplasm.
Domain:
The N-terminal 423 amino acids, which includes the OTU deubiquitinase domain and the tudor domain, is essential for germ cell proliferation and early germ cell differentiation up to stage 10 of oogenesis; Possesses a C-terminal low complexity (LC) domain that promotes protein coalescence, probably by forming amyloid-like aggregates. Protein coalescence mediated by this domain is indispensable for deubiquitinase activity. The C-terminal domain is required for oocyte maturation.
Sequence:
MDMQVQRPITSGSRQAPDPYDQYLESRGLYRKHTARDASSLFRVIAEQMYDTQMLHYEIRLECVRFMTLKRRIFEKEIPGDFDSYMQDMSKPKTYGTMTELRAMSCLYRRNVILYEPYNMGTSVVFNRRYAENFRVFFNNENHFDSVYDVEYIERAAICQSIAFKLLYQKLFKLPDVSFAVEIMLHPHTFNWDRFNVEFDDKGYMVRIHCTDGRVFKLDLPGDTNCILENYKLCNFHSTNGNQSINARKGGRLEIKNQEERKASGSSGHEPNDLLPMCPNRLESCVRQLLDDGISPFPYKVAKSMDPYMYRNIEFDCWNDMRKEAKLYNVYINDYNFKVGAKCKVELPNETEMYTCHVQNISKDKNYCHVFVERIGKEIVVPYESLHPLPPDEYRPWSLPFRYHRQMPRLPLPKYAGKANKSSKWKKNKLFEMDQYFEHSKCDLMPYMPVDNCYQGVHIQDDEQRDHNDPEQNDQNPTTEQRDREEPQAQKQHQRTKASRVQPQNSSSSQNQEVSGSAAPPPTQYMNYVPMIPSRPGHLPPPWPASPMAIAEEFPFPISGTPHPPPTEGCVYMPFGGYGPPPPGAVALSGPHPFMPLPSPPLNVTGIGEPRRSLHPNGEDLPVDMVTLRYFYNMGVDLHWRMSHHTPPDELGMFGYHQQNNTDQQAGRTVVIGATEDNLTAVESTPPPSPEVANATEQSPLEKSAYAKRNLNSVKVRGKRPEQLQDIKDSLGPAAFLPTPTPSPSSNGSQFSFYTTPSPHHHLITPPRLLQPPPPPPIFYHKAGPPQLGGAAQGQTPYAWGMPAPVVSPYEVINNYNMDPSAQPQQQQPATLQPAPLSVQSQPAAVYAATRHH