The following text describes a protein:
Function:
The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity); [3C-like proteinase nsp5]: Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity); Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter; [RNA-capping enzyme subunit nsp9]: Catalytic subunit of viral RNA capping enzyme which catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. The kinase-like NiRAN domain of NSP12 transfers RNA to the amino terminus of NSP9, forming a covalent RNA-protein intermediate. Subsequently, the NiRAN domain transfers RNA to GDP, forming the core cap structure GpppA-RNA. The NSP14 and NSP16 methyltransferases then add methyl groups to form functional cap structures; [Non-structural protein 1]: Binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).
Miscellaneous:
MISCELLANEOUS: [Isoform Replicase polyprotein 1a]: Produced by conventional translation.
Subcellular Localization:
[Papain-like protease nsp3]: Host membrane; Multi-pass membrane protein; [Non-structural protein 4]: Host membrane; Multi-pass membrane protein; [Non-structural protein 6]: Host membrane; Multi-pass membrane protein; [Non-structural protein 7]: Host cytoplasm, host perinuclear region. Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity); [Non-structural protein 8]: Host cytoplasm, host perinuclear region. Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity); [RNA-capping enzyme subunit nsp9]: Host cytoplasm, host perinuclear region. Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity); [Non-structural protein 10]: Host cytoplasm, host perinuclear region. Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).
Domain:
The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.
Sequence:
MAKMGKYGLGFKWAPEFPWMLPNASEKLGNPERSEEDGFCPSAAQEPKVKGKTLVNHVRVDCSRLPALECCVQSAIIRDIFVDEDPQKVEASTMMALQFGSAVLVKPSKRLSVQAWAKLGVLPKTPAMGLFKRFCLCNTRECVCDAHVAFQLFTVQPDGVCLGNGRFIGWFVPVTAIPEYAKQWLQPWSILLRKGGNKGSVTSGHFRRAVTMPVYDFNVEDACEEVHLNPRGKYSCKAYALLRGYRGVKPILFVDQYGCDYTGCLAKGLEDYGDLTLSEMKELSPVWRDSLDNEVVVAWHVDRDPRAVMRLQTLATVRSIEYVGQPIEDMVDGDVVMREPAHLLAPNAIVKRLPRLVETMLYTDSSVTEFCYKTKLCDCGFITQFGYVDCCGDTCGFRGWVPGNMMDGFPCPGCCKSYMPWELEAQSSGVIPEGGVLFTQSTDTVNRESFKLYGHAVVPFGGAAYWSPYPGMWLPVIWSSVKSYSYLTYTGVVGCKAIVQETDAICRFLYMDYVQHKCGNLEQRAILGLDDVYHRQLLVNRGDYSLLLENVDLFVKRRAEFACKFATCGDGLVPLLLDGLVPRSYYLIKSGQAFTSLMVNFSREVVDMCMDMALLFMHDVKVATKYVKKVTGKVAVRFKALGIAVVRKITEWFDLAVDTAASAAGWLCYQLVNGLFAVANGVITFIQEVPELVKNFVDKFKTFFKVLIDSMSVSILSGLTVVKTASNRVCLAGSKVYEVVQKSLPAYIMPVGCSEATCLVGEIEPAVFEDDVVDVVKAPLTYQGCCKPPSSFEKICIVDKLYMAKCGDQFYPVVVDNDTVGVLDQCWRFPCAGKKVVFNDKPKVKEVPSTRKIKIIFALDATFDSVLSKACSEFEVDKDVTLDELLDVVLDAVESTLSPCKEHGVIGTKVCALLERLVDDYVYLFDEGGEEVIASRMYCSFSAPDEDCVATDVVYADENQDDDADDPVVLVADTQEEDGVAREQVDSADSEICVAHTGGQEMTEPDVVGSQTPIASAEETEVGEACDREGIAEVKATVCADALDACPDQVEAFDIEKVEDSILSELQTELNAPADKTYEDVLAFDAIYSETLSAFYAVPSDETHFKVCGFYSPAIERTNCWLRSTLIVMQSLPLEFKDLGMQKLWLSYKAGYDQCFVDKLVKSAPKSIILPQGGYVADFAYFFLSQCSFKVHANWRCLKCGMELKLQGLDAVFFYGDVVSHMCKCGNSMTLLSADIPYTFDFGVRDDKFCAFYTPRKVFRAACAVDVNDCHSMAVVDGKQIDGKVVTKFNGDKFDFMVGHGMTFSMSPFEIAQLYGSCITPNVCFVKGDVIKVLRRVGAEVIVNPANGRMAHGAGVAGAIAKAAGKAFINETADMVKAQGVCQVGGCYESTGGKLCKKVLNIVGPDARGHGNECYSLLERAYQHINKCDNVVTTLISAGIFSVPTDVSLTYLLGVVTKNVILVSNNQDDFDVIEKCQVTSVAGTKALSFQLAKNLCRDVKFVTNACSSLFSESSFVSSYDVLQEVEALRHDIQLDDDARVFVQANMDCLPTDWRLVNKFDSVDGVRTIKYFECPGEVFVSSQGKKFGYVQNGSFKEASVSQIRALLANKVDVLCTVDGVNFRSCCVAEGEVFGKTLGSVFCDGINVTKVRCSAIHKGKVFFQYSGLSAADLAAVKDAFGFDEPQLLQYYSMLGMCKWPVVVCGNYFAFKQSNNNCYINVACLMLQHLSLKFPKWQWRRPGNEFRSGKPLRFVSLVLAKGSFKFNEPSDSTDFIRVELREADLSGATCDLEFICKCGVKQEQRKGVDAVMHFGTLDKSGLVKGYNIACTCGDKLVHCTQFNVPFLICSNTPEGKKLPDDVVAANIFTGGSVGHYTHVKCKPKYQLYDACNVSKVSEAKGNFTDCLYLKNLKQTFSSVLTTYYLDDVKCVAYKPDLSQYYCESGKYYTKPIIKAQFRTFEKVEGVYTNFKLVGHDIAEKLNAKLGFDCNSPFMEYKITEWPTATGDVVLASDDLYVSRYSGGCVTFGKPVIWRGHEEASLKSLTYFNRPSVVCENKFNVLPVDVSEPTDRRPVPSAVLVTGAASGADASAISTEPGTAKEQKACASDSVEDQIVMEAQKKSSVTTVAVKEVKLNGVKKPVKWNCSVVVNDPTSETKVVKSLSIVDVYDMFLTGCRYVVWTANELSRLINSPTVREYVKWGMSKLIIPANLLLLRDEKQEFVAPKVVKAKAIACYGAVKWFLLYCFSWIKFNTDNKVIYTTEVASKLTFKLCCLAFKNALQTFNWSVVSRGFFLVATVFLLWFNFLYANVILSDFYLPNIGPLPMFVGQIVAWVKTTFGVLTICDFYQVTDLGYRSSFCNGSMVCELCFSGFDMLDNYESINVVQHVVDRRVSFDYISLFKLVVELVIGYSLYTVCFYPLFVLVGMQLLTTWLPEFFMLGTMHWSARLFVFVANMLPAFTLLRFYIVVTAMYKVYCLCRHVMYGCSKPGCLFCYKRNRSVRVKCSTVVGGSLRYYDVMANGGTGFCTKHQWNCLNCNSWKPGNTFITHEAAADLSKELKRPVNPTDSAYYSVIEVKQVGCSMRLFYERDGQRVYDDVSASLFVDMNGLLHSKVKGVPETHVVVVENEADKAGFLNAAVFYAQSLYRPMLMVEKKLITTANTGLSVSRTMFDLYVYSLLRHLDVDRKSLTSFVNAAHNSLKEGVQLEQVMDTFVGCARRKCAIDSDVETKSITKSVMAAVNAGVEVTDESCNNLVPTYVKSDTIVAADLGVLIQNNAKHVQSNVAKAANVACIWSVDAFNQLSADLQHRLRKACVKTGLKIKLTYNKQEANVPILTTPFSLKGGAVFSRVLQWLFVANLICFIVLWALMPTYAVHKSDMQLPLYASFKVIDNGVLRDVSVTDACFANKFNQFDQWYESTFGLVYYRNSKACPVVVAVIDQDIGHTLFNVPTKVLRYGFHVLHFITHAFATDRVQCYTPHMQIPYDNFYASGCVLSSLCTMLAHADGTPHPYCYTEGVMHNASLYSSLVPHVRYNLASSNGYIRFPEVVSEGIVRVVRTRSMTYCRVGLCEEAEEGICFNFNSSWVLNNPYYRAMPGTFCGRNAFDLIHQVLGGLVQPIDFFALTASSVAGAILAIIVVLAFYYLIKLKRAFGDYTSVVVINVIVWCINFLMLFVFQVYPTLSCLYACFYFYTTLYFPSEISVVMHLQWLVMYGAIMPLWFCITYVAVVVSNHALWLFSYCRKIGTDVRSDGTFEEMALTTFMITKESYCKLKNSVSDVAFNRYLSLYNKYRYFSGKMDTATYREAACSQLAKAMETFNHNNGNDVLYQPPTASVTTSFLQSGIVKMVSPTSKVEPCVVSVTYGNMTLNGLWLDDKVYCPRHVICSSADMTDPDYPNLLCRVTSSDFCVMSDRMSLTVMSYQMQGSLLVLTVTLQNPNTPKYSFGVVKPGETFTVLAAYNGRPQGAFHVVMRSSHTIKGSFLCGSCGSVGYVLTGDSVRFVYMHQLELSTGCHTGTDFSGNFYGPYRDAQVVQLPVQDYTQTVNVVAWLYAAILNRCNWFVQSDSCSLEEFNVWAMTNGFSSIKADLVLDALASMTGVTVEQVLAAIKRLHSGFQGKQILGSCVLEDELTPSDVYQQLAGVKLQSKRTRVIKGTCCWILASTFLFCSIISAFVKWTMFMYVTTHMLGVTLCALCFVIFAMLLIKHKHLYLTMYIMPVLCTLFYTNYLVVGYKQSFRGLAYAWLSYFVPAVDYTYMDEVLYGVVLLVAMVFVTMRSINHDVFSTMFLVGRLVSLVSMWYFGANLEEEVLLFLTSLFGTYTWTTMLSLATAKVIAKWLAVNVLYFTDIPQIKLVLLSYLCIGYVCCCYWGVLSLLNSIFRMPLGVYNYKISVQELRYMNANGLRPPRNSFEALMLNFKLLGIGGVPVIEVSQIQSRLTDVKCANVVLLNCLQHLHIASNSKLWQYCSTLHNEILATSDLSVAFDKLAQLLVVLFANPAAVDSKCLASIEEVSDDYVRDNTVLQALQSEFVNMASFVEYELAKKNLDEAKASGSANQQQIKQLEKACNIAKSAYERDRAVARKLERMADLALTNMYKEARINDKKSKVVSALQTMLFSMVRKLDNQALNSILDNAVKGCVPLNAIPPLTSNTLTIIVPDKQVFDQVVDNVYVTYAPNVWHIQSIQDADGAVKQLNEIDVNSTWPLVISANRHNEVSTVVLQNNELMPQKLRTQVVNSGSDMNCNIPTQCYYNTTGTGKIVYAILSDCDGLKYTKIVKEDGNCVVLELDPPCKFSVQDVKGLKIKYLYFVKGCNTLARGWVVGTLSSTVRLQAGTATEYASNSAILSLCAFSVDPKKTYLDYIQQGGVPVTNCVKMLCDHAGTGMAITIKPEATTNQDSYGGASVCIYCRSRVEHPDVDGLCKLRGKFVQVPLGIKDPVSYVLTHDVCQVCGFWRDGSCSCVGTGSQFQSKDTNFLNGFGVQV