The following text describes a protein:
Function:
Non-ribosomal peptide synthetase, part of the diffuse TOX2 gene cluster that mediates the biosynthesis of the HC-toxin, cyclic tetrapeptide of structure cyclo(D-Pro-L-Ala-D-Ala-L-Aeo), where Aeo stands for 2-amino-9,10-epoxi-8-oxodecanoic acid. HC-toxin is a determinant of specificity and virulence in the interaction between the producing fungus and its host, maize. HTS1, contains four modules, one for each amino acid in HC-toxin, with the order of activation being most likely Pro, Ala, Ala, and Aeo. In addition, HTS1 has one epimerase domain between modules 1 and 2, which is responsible for epimerizing L-Pro to D-Pro. The absence of an epimerizing domain after module 3, for producing D-Ala, can be explained by the presence in the cluster of TOXG, an Ala racemase, which produces D-Ala for incorporation by HTS1 into HC-toxin.
Miscellaneous:
MISCELLANEOUS: The genes involved in HC-toxin biosynthesis, called collectively TOX2, are organized into a diffuse cluster that spans >500 kb. All of the known genes are duplicated or triplicated within this region, with some variation in copy number and chromosomal location among different race 1 strains.
Domain:
NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for L- to D-amino acid conversion) are present within the NRP synthetase. HST1 has the following tetrameric architecture: A-T-C-E- A-T-C-A-T-C-A-T-C.
Sequence:
MTMPHHSSGPAKDSPLCRFPPFPGGNPVFTNIRREKVNFQLPPPDHALLAAAWAVLLRLYTGHVKTCFESATSDQEANLVTYEARDSDTLQTIVLRGACVSSTAEEKAGLRDLNTAVVRTTVSIDSWTDEMLQDKIAALLQPGKEIVLFQTPSGCVLVYMQSFMSAMEVKNVSSTLTYIMSSDPDKTAIRNLSISPRDLAQIMRWNDRKLKSERTNLVYDLFSARAHEQDANMAIDAWDGRMSYTELERVSSTWARQLQKQGISQGSWVLFCFEKSRLAVVSMIAILKAGGVCVPIDPRYPVERIRDIIRTTNATIALVGAGKTAALFKSADTAVQTIDITKDIPHGLSDTVVQSNTKIDDPAFGLFTSGSTGVPKCIVVTHSQICTAVQAYKDRFGVTSETRVLQFSSYTFDISIADTFTALFYGGTLCIPSEEDRMSNLQDYMVSVRPNWAVLTPTVSRFLDPGVVKDFISTLIFTGEASREADTVPWIEAGVNLYNVYGPAENTLITTATRIRKGKSSNIGYGVNTRTWVTDVSGACLVPVGSIGELLIESGHLADKYLNRPDRTEAAFLSDLPWIPNYEGDSVRRGRRFYRTGDLVRYCDDGSLICVGRSDTQIKLAGQRVELGDVEAHLQSDPTTSQAAVVFPRSGPLEARLIALLVTGNKDGTPHNQQSLPKPAFAQCPPDLVKYATSSLQQRLPSYMVPSVWLGIDFLPMSVSGKLDRAVLQDQLESLSPSDYAEILGTTGLEVDPGGAASSVASDSDLRDMNDDSLLLTACSRVLNLPAGKISYSQSFIHAGGDSITAMQVSSWMKRFTGKRIGVKDLLVSPSISTAASCIKSAQDGSRNFVAVRPGQRIPVSPIQKLFFQTAEASKSWNHYHQSFLFRIDQPIKPQTIEDAISLVMQRHPMLQARFERTEEGDWYQYIPIDVERRASVEVIGSLSTDDREAAMLRARQSIDLTEGPLIRCQLFNNNVDEASRLFFVVIHHAVVDLVSWRIIMEELEAHLATDSTPDRGEAYQESVPFLAWCQVQAEAVKDIPVDRTVPLIPKIPTADFGYWGLKHDENVYGNTVERKIPLGHSITEDLLYKCHDSLHTKTIDVLLAAVLVSFRRSFLDRPVPAVFNEGHGREPGGEDAVDLSRTVGWFTTISPVYVPEVSPGDILDVVRRVKDYRWATPNNGFDYFSTKYLTQSGIKLFEDHLPAEILFNYEGRYQAMESEQTVLKPESWHAGEASKDQDPGLRRFCLFEISTAVLPDGQLHLTCSWNKNMRHQGRIRLWLDTLLPAAIGEIVSSLALASPQLTLSDVELLRLYDYSSLDILKKSILSIPAVQTLDDLEGVYPGSPMQDALFLSQSKSQDGAYEVDFTWRVATSLQNSQPAVDIGCLVEAWKDTVALHAALRTVILESSLPATGILHQVVLRSHDPDIVILDVRDVTAAITILDSYPPPTEEGIALIKRPPHRLLICTTIEGSVLIKFQVNHLVFDGMSTDKIIQDLSKAYTCRHSNKLPDHSESKLHDGTYGNRPTKPPLAEFIRYIRDPQRKQDSINYWKNALRGATTCSFPPLFDQITSEKAMPRQSWASVPIPLCVDSKELSKTLANLGITMSTMFQTVWAIVLRIYSQNGQSVFGYLTSGRDAPVDGIDSAVGNFIAMLVCFFDFDDDGVHTVADMARKIHNASANSISHQACSLAEIQDALGLSTSTPLFNTAFTYLPKRPTNVKAGEPEHHLCFEELSMSDPTEFDLTLFVEPTQESNEVSAHLDFKLSYISQAYATSIASTVAHILSELVHDPYRALNTLPIVSEHDTAIIRSWNDHLFPPATECIHETFSRKVVEHPQREAICSWDGSLTYAELSDLSQRLSIHLVSLGIKVGTKIPICFEKSMWTIVTILAVVQAGGVFVLLEPGHPESRLSGIIKQVQAELLLCSPATSRMGALQNISTQMGTEFKIVELEPEFIRSLPLPPKPNHQPMVGLNDDLYVVFTSGSTGVPKGAVATHQAYATGIYEHAVACGMTSLGAPPRSLQFASYSFDASIGDIFTTLAVGGCLCIPREEDRNPAGITTFINRYGVTWAGITPSLALHLDPDAVPTLKALCVAGEPLSMSVVTVWSKRLNLINMYGPTEATVACIANQVTCTTTTVSDIGRGYRATTWVVQPDNHNSLVPIGAVGELIIEGSILCRGYLNDPERTAEVFIRSPSWLHDLRPNSTLYKTGDLVRYSADGKIIFIGRKDTQVKMNGQRFELGEVEHALQLQLDPSDGPIIVDLLKRTQSGEPDLLIAFLFVGRANTGTGNSDEIFIATSTSSLSEFSTVIKKLQDAQRAMEVLPLFMVPQAYIPIEGGIPLTAAGKIDRRMLRKLCEPFNRNDLISFTSKALSTSVKDAETTDTVEDRLARIWEKVLGVKGVGRESDFFSSGGNSMAAIALRAEAQRSGFTLFVADIFTNPRLADMAKLFSHGQSVSPSSSTLRTKVPISSLQKRSSGLQTAAPVSNGSPVRRCQKENIIDCPVAFEYEEGPSDTQLKEASRICGISSRSIEDVFPCTPMQEALVALSLIPGAQASYALHAAFELRPGLDRNRFRSAWESTVKAQPILRSRIISGSNGSSVVVTSATDSIPQLDVSGLDTFLEQQLQVGFAPGAPLFRLAFVYSKADDCDYFVISAHHAIYDGWSLNLIWSQVLALYTNGELPPPGPSFKHFARNLNLVQSKLDSEDFWRKLLVKPDQESFRFPDVPVGHKPATRCTTNFHFPFSMQSKIGTTANTCINAAWAITLAQYSSNKTVNFGVTLWGRDFPMIDIEHMTGPTIVTVPRQVNVIPESSVAEFLQDLQKSLAVVLPHQHLGLHRIQALGPIARQACDFSTLLVVNHGSSISWSELEAADIVPVPLRSSDLYAYPMVVEVENASSDTLDIRVHSDPDCIEVQLLERLMEQFGHNLQTLCRAASFDPGKRIAELMDDTATTHLRTLFSWNSRVKDSPDVAAIAVHKLLEETAQSQPAESAIVAHDGQLSYMQMDRCADVLARQIRKTNMISAQSPFVCIHLLRSATAVVSMLAVLKAGGAFMPVDISQPRSRLQNLIEESGAKLVLTLPESANALATLSGLTKVIPVSLSELVQQITDNTTKKDEYCKSGDTDPSSPAYLLYTSGTSGKPKGVVMEHRAWSLGFTCHAEYMGFNSCTRILQFSSLMFDLSILEIWAVLYAGGCLFIPSDKERVNNLQDFTRINDINTVFLTPSIGKLLNPKDLPNISFAGFIGEPMTRSLIDAWTLPGRRLVNSYGPTEACVLVTAREISPTAPHDKPSSNIGHALGANIWVVEPQRTALVPIGAVGELCIEAPSLARCYLANPERTEYSFPSTVLDNWQTKKGTRVYRTGDLVRYASDGTLDFLGRKDGQIKLRGQRIELGEIEHHIRRLMSDDPRFHEASVQLYNPATDPDRDATVDVQMREPYLAGLLVLDLVFTDEVMGIPCTSLTSANTSENLQTLVTELKKSLRGVLPHYMVPLHFVAVSRLPTGSSGKLDHAFVRACLRELTAPLDGNFPKVEQVLTTNESVLRQWWGTVLAMDPHSIQRGDDFFSLGGSSISAMRLVGLARSSGHKLQHEDIFMCPRLADMAGQISFVQEASVSPTTSPTIKFDLLDDCEVDEVIDHILPQLDMNKELIEDVYPCTPLQESLMAATARHGEAYTMIQSITVLASQLAQLKKAMDVVFRDFEVLRTRIALGPSQQALQVVVKHEELSWESFPSIQSFKDHFYRSLGYGKPLARLAVITQALDTKQPISHGTREARTKNSQDTVMVVVGAHHSIYDAHVLSMIWRRLYREFIGSQADGILEAETSRSEGVVPFKSYVEKLLRGKDNDESLLFWKEKLRGVSSSQFPPASWPRVLEHQPSATQTLITKVSLPTSSRKKLGATVATVAYAAWALTIAHYTADPDVVFGATLSGRETMAGSISHPESIAGPTIITVPLRIIIDFQTVVSDFLSTLQKDIVRAAYFGQMMGLNSIAHIDNDCRDACGFKSIIVVQVPDEGENHDGRAANPFQMSLESIGHFPAPLVVEVEQSESTDVLIRMAYDPVLVPEKLAHFISDTFTTTMSNLSAANPKAKVESIPALSEAHLAELDVTCPEWILGKAKDEKIRTESHQCLQDLVCRRAQQSPNSQAIDSWDGSISYHELDGLSSILAEHLSQLGVRPEAPVCLLFEKSKWAVVAMIGIIKAGGCFVPLDPSYPHERLEHIISETGSSVIVTSAAYSKLCLSLSVRGIVCDGSVFSSTKKPLPSTADSPPSFSVRPNQAAYILFTSGSTGKPKGVVMEHHSVCSALIALGKRMGLGPQSRVLQFNSYWFDVMLLDIFGTLVYGGCLCIPKEEQRMSNLSGWVQKFKVNTMLLSTSVSRLMQPADTPSLETLCLTGEAVLQSDVDRWAPKLHLIAGYGPTETCIMSVSGELTPSSPANLIGKPVSCQAWVINPLKETELAPYGATGELYIQGPTVARGYLHDDVLTSKAFIVDPQWLTGYKTNENQWSRRAYKTGDLVFWGPQSNLYYVRRKDSSQVKIRGQRVELAEIEEVIRQHIPPDVTVCVDLLSSDDQNTRIILGAVLGIGDRALGGPEDLEVIGYMDDLKSHIIPALEASLPHHMIPEAYVPFVQLPTLGSGKLDRKTVRRVAGPLAFSLPQASARHPNQPTVTHTQKLLRQLWCKILPQLDESAVNKQDNFLGIGGDSIAAIKLVALLRQHGISLAVAEIFTRPTLEAMSSLIDEHNFVVSHAGILSDVTRNTSGVMRQTTNLIAGRHSMAVEKSRECDNSTLPCTEYQQMFLAGTEAFTGAHSAQFIFRLPEKIDLDRLQAAFDHCADWYPNLRTQIHKDADTGRLLHDISPIGVKVPWSCHYSDDLNTVLSHDKKFPPGLDGPLHRVTIMRHRDPTESMLVWTLNHAAYDAWSLRMMLEHITEAYANPDYEPSYSLGWTAFVLHTENTKEASRSFWSSYLSDVKPARLMFNYNLVSNPRQDRLYEARINIPKRVLSQATAATVLLAGLTLLVARVCDTRDVILAHLLTGRTLPLAGIENCPGPTITKVPLRIPLMDQDLVTLELDSVAKKITAELMRVMPHEHSGLSAIREFIPQAEGTTTSSGKFHAGSVLGRLPLDLVIHPKGGLDLLGKHGLGLQNEGFRLVAPPSGGLSMECALVDDDDDKRSDTISVDVSVLWDQRAATQEDVIELVHSLQGIFTKRNLAASICLMYK