The following text describes a protein:
Function:
Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of meroterpenoids called sartorypyrones. The biosynthesis of sartorypyrones begins with the production of triacetic acid lactone (TAL) by the NR-PKS spyA using one molecule of acetyl-CoA and two molecules of malonyl-CoA. As spyA lacks a thioesterase (TE) domain, TAL is likely generated through self-release from spyA by spontaneous lactonization. After production of TAL, the prenyltransferase spyF then conjugates geranylgeranyl pyrophosphate (GGPP) to TAL to form geranylgeranyl-triacetate lactone, for which the pathway-specific geranylgeranyl pyrophosphate synthase (GGPS) spyE is required to provide GGPP. Subsequently, geranylgeranyl-triacetate lactone is epoxidized at the terminal olein by the FAD-dependent monooxygenase spyC, followed by cyclization of the terpenoid component catalyzed by the terpene cyclase spyD to produce both the bicyclic sartorypyrone F and the monocyclic sartorypyrone D. Finally, the last step of the biosynthesis involves the acetylation of the meroterpenoids sartorypyrones D and F by the acetyltransferase SpyB to produce sartorypyrones A and G, respectively.
Domain:
Multidomain protein; including a starter unit:ACP transacylase (SAT) that selects the starter unit; a ketosynthase (KS) that catalyzes repeated decarboxylative condensation to elongate the polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects and transfers the extender unit malonyl-CoA; a product template (PT) domain that controls the immediate cyclization regioselectivity of the reactive polyketide backbone; and 2 acyl-carrier protein (ACP) domains that serve as the tether of the growing and completed polyketide via its phosphopantetheinyl arm.
Sequence:
MASTLPSTVVCGSQTSPPSRDCLQRIQADLKDPKLKELAKAVDELPEFWSHLVEVKPVLSQVNDAPLKKLKDWAKDESVLNIPEGLPNILLAPLTVIIHLVQYLRFAHSLDANDSQEDQHHRILQSTSKGGFQGLCTGFLSAAALACSRTRSDIWCNATVALRLATCVGAYVDLDRLTYDNTDVHTCMIIHWEDNCGKDQVTEVLKGYPGVYISVELDNRSITVTAKQSQVSLLRGDLAARGAKLTRIPIYGRYHSRTHAEILQDLLEICSNDESLRFPIISAPIVPLRNNTTGGLITSAEKLHELALKCMLTEPADWYSTMSETVSSLMLQPVINEHPLILELGHVSCLPRSLTASANIRAFTPLLTLTASPNEPDASRYDYPEHSIAVIGAACKFTGAETMQQFWELIRAGGTMVGELPEGRIALDKKSLRKPPREEPLRGNFLSRAGHFDHGLFGLSQREARYMDPQQRIALQVAYHAVESSEYFRSGIKDKNVGCYVGVGGSDYDHNVCSHAPTAFSFTGTARAFVSGRISHHFGWTGPSMTIDTACSSSAVAIHQACKDIRMGECRMALAGGVNIISCPNMQQNLAAARFLSPTGGPCRPFDAFADGYCRGEGCGFVMLKKLSCAVADKDEILGVIVGSAANHCDGNDAITVPKSHSQIDLYRKALSLAAMQPKDITYVEAHGTGTSRGDPVECRSIRQVFGGERPTLHFGSVKANVGHTEAASGIAGLLKVLLMIRNQRIPPQANFTQLNSSIPPLEPDNLSITTNELEWKATFRAACVNNYGAAGNNTAVIVCEPPRLKPSAGNNHILSGYPFLITGQSGRSLKMYCTALGEWLENTDAPLSDIAFSVTRRQNRTLRRRITFAARSLKDMKNILGQQARLDQQSSPSPKPKPVVLVFAGQTGNVVYLHKDAYEGSSLLRSHIDECDKILREMGLRGLIAFLFDQQPIEDVVLLHCLFFSLQYACAAAWLDAGLPVQRVIGHSIGQFTAMCISGVTTLADTLKIVAGRARLIQQKWAKERGCMLAIEVDRQGALELARSVPGHKIEVACFNGARNHVLVGTEVAIQTLEAKTSCKTRRLKTTHGFHSELVDHWLDDYMALAQSITYATPIIPIETCSEGGSWKEFTPEMLPRHSREAVYFFDAISRVDKELGPCTWVEGGVGSKGIALVKCALGTNTSGHSFHRLQLDSSEPLASLTDTTIQMWDEGVDVQFWLYHDTEKITFDSCDVPAYQFDETEFWLLRSDQARQSDLQRGRVISDRYKPLVYLLRDEGFRGEPRKLHFGVNQYHPDFDRYLHGREVLGEVLCPVSVFVELAAKAAAICGGRECSTAATQVQVLNLEIHNPLGHAPGARVGLRLKRIGSCKWGFSVISDKDVQSICHATGVISLRQERHGKLEIGPQWSVIQRVIGYSHIQDVLDDSSAISLKEGIIYKVLEKVAEYKPCHRAIKSMTIKGTAAVAQLEMPAISRDSSEITVSDPLVMDQFALIAEVLALCRDDCKRDDVFICSGLSELVTFKSLQSGLGPWTVYTQQTPCGPRELLSDTFVFDSTSKQLIIGLLGARFVRITASSLNGIVKRANLNSQTSELSVETDAVNHPASVAYPPSNNNDTSDILSILSHLLHEITGLPPSEISASTPLMEASVDSLAATELENRIQEEFHLHRPISLYERQFNVGTLCQEIQTQRDDRLARASRTTTATRNTSFSLGRRTSSTESLPDDAEARFISKSAAVLAQLSRMLSESFNITESILPGTELRSLGLDSLVAVELEYDLQQAFGLKVDLMQLSPELTVGGLARLILASESQTSGHIASSMMDCRQTTEGKSQMYLG