The following text describes a protein:
Function:
Nonribosomal peptide synthetase; part of the siderophore biosynthetic pathway. Aspergillus fumigatus produces four types of siderophores, low-molecular-mass iron chelators, including excreted fusarinine C (FsC) and triacetylfusarinine C (TAFC) for iron uptake; and intacellular ferricrocin (FC) for hyphal and hydroxyferricrocin (HFC) for conidial iron distribution and storage. TAFC consists of three N(2)-acetyl-N(5)-anhydromevalonyl-N(5)-hydroxyornithine residues cyclically linked by ester bonds; FC is a cyclic hexapeptide with the structure Gly-Ser-Gly-(N(5)-acetyl-N(5)-hydroxyornithine)x3. The biosynthesis of all four siderophores depends on the hydroxylation of ornithine, catalyzed by the monooxygenase sidA. Subsequently, the pathways for biosynthesis of extra- and intracellular siderophores split. For biosynthesis of extracellular siderophores, the transacylase sidF transfers anhydromevalonyl to N(5)-hydroxyornithine. The required anhydromevalonyl-CoA moiety is derived from mevalonate by CoA ligation and dehydration catalyzed by sidI and sidH respectively. The acetylation of N(5)-hydroxyornithine for FC biosynthesis involves the constitutively expressed sidL. FC is hydroxylated to HFC by an as yet uncharacterized enzyme during conidiation. Assembly of fusarinine C (FsC) and FC is catalyzed by two different nonribosomal peptide synthetases (NRPS), sidD and sidC respectively. Subsequently, sidG catalyzes N2-acetylation of FsC for forming TAFC. Both extra- and intracellular siderophores are crucial for growth during iron limitation and virulence.
Domain:
NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for l- to d- amino acid conversion) are present within the NRP synthetase. NRPS4 has the following architecture: A-T-C-A-C.
Sequence:
MGSIQQDDVHNQIDHCNQSDDLPAARLNCNDVELFEVAGLACDETSSPTGMRDEMVLLSWLIALLRTREGGQIRYEWAYRYPEEEPVPRCLAMNEVVAGLQSSVKETAAAVSRHISADVSSPPAPASLLLSTSSLSQTSDEAKDEGLLHLEIAFENGLCKIRPTWHSENMLPFTVTRYARTLIDTVRLCISNCDAAIQDCLRPTAYDLDEIWRWNHNLPPTYNFCMHEIISDQAQKFPDKEAIASWDGSLTYRQIDQYSSFVARSLIGMGVGLHDVLPVCFEKSRWTIVAVLAVMKAGATFVLMDPTLPLARLQNMAQQVGAKMMVSSRGQYNLATEIIPNANVLVVEENTFSSLSAEQNGEPLPTVPSSALMYMIFTSGSTGTPKGVKISHETYTSSAIPRANAVGYTEDSRVLDFASYAFDVSIDSMLLTLGNGGCLCIPSDEDRLNDINGVIRRMKVNYAGLTPSVARILDADVISSLSGLGLGGEAVSARDVNLWGQDTRIIIGYGPCECTIGCTVNSSAATGRDYISIGPGNGAVIWIVDPNDHESLVPLGAVGELLVEGPIVGQGYLNDPEKTAAAFIEDPSWLVAGHEGYPGRRGRLYKTGDLGRYDPDGSGGIVFVGRKDTQVKLRGQRVELGEIESQLRARLPSETTVIAEVIVPQGSGGQPTLVAFVAAQTTKGHDHTGLEAAELPDELRRALSEADAELAKVLPRYMVPTAYIPVNHIPTLISGKTDRKRLRQFGATVDLRQLDQDATNTAARELSDLERRLRQAWSQTLKLQACSIRLQDNFFALGGDSLTAMKLVSVCRSQGLDLSVTSMFSNPTLSAMASVVRICDVDVQRTVPAFSMITSDMNSACVEAAEPCGVGPADIEDIYPCTPTQESLFTFSLKSVKPYVAQRVLCIPSHIDLNAWRKAWEDVVAALPILRTRVAQLQEPGLQQVVLKNSISWTQASDLAEYLENDRTQKMNLGESLARYAIVEDSADGKRYMVWTIHHVLYDGWSEPIILKQVSDALQGQPVEVKAQMRDFVRFVRDSDDAAVQEFWRRELKGAVGPQFPRLPSRDFMPTPDALVERQVSLDTSSGSPFTMATLIRGAWALVASQYTGSDDIVFGETLTGRDIPLPGVESIVGPLIATVPIRVRILRGSTVESYLQAVQQSVLARTPYQHLGMQNIRKVSQDAQHACETGTGLVIQPEPEYVGSELGVERGDVVLEALHFNPYPLMLACGIRKGGFRVCASFDSSLIETRQMERMLAQLETACWQLSQGLSRKVDEISCLPEAELNQIWQWNRSPPLSLDETTSRLRANASTKPGSSYPPAVVPWVCSPRNSSLLSPIGCVGELWLEGALLSGDTVDSPAWLVAGSSTCAGRTGKVQATGDMVQLREDGSLVFVGRKENVVPVQGHAVDITEIERHLAEHLPPTIRAAATVVRSSSDQELVMFIEQPAAEEACIELLSEKREIVCDAPDKAFQTTICATIPGSLAAVLKKLDKYMRDSLPSYMAPSAYIVVEKLPNTMDDIDHNLLNQIASQVTPQILNELRDGLSNAWTKATAPNHLSASESILRSAWAKVLRVDPEQIDVDDNFFRRGGDSVLAMKLVSSLRAQGYSLSVADIFRHMRLSDAARVMKVDERSTEKINSYQPFSMLRLPDVQQFLANIVRPQLGDQHWPIRDVLPVTDSQDMDIRATIQPPRTSIQYTMLYFDNSVDRERLFRSCSDLVKTHEILRTVFISHESSFLQVVLNELEIPVRAHKTDKQLDQYVASLFREDIESNFQLGCPFLRLFYVEGNNGESCLVIGLSHAQYDGVSLPRLLQDLDALYTGTQLATFSPFSLYMAQTSEEAIQNKAAAYWRNLLSSSSLSTLDGPSSDPTDKAIFHTRPVNIHPLKEITTANLLTAAWAMVLARRLQTPDVTFGSVTSGRTLDIPNAENFMGPCYQLTPVRVPFHPDWTASDLLNFVQTQSAESAAHDFLGFEKIAKLAGWASGRQGFDSIVHHQDWEDFDMMPFGGGSCRVDIANPHGDAAYPVKAVSFVKEGEIHVGVVCSERDVMFVDEVLGELAAAVVELAGQSTEVLLDSKLFSGQ