The following text describes a protein:
Function:
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of penicillin, the world's most important antibiotic. The trimodular NRPS PCBAB produces the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) via condensation of the 3 residues L-2-aminoadipate, L-cysteine and L-valine. The precursor amino acids for penicillin biosynthesis are withdrawn from the vacuolar amino acid pool by the MFS-type transporter penV (By similarity). Each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. The penicillin biosynthesis occurs via 3 enzymatic steps, the first corresponding to the production of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) by the NRPS PCBAB. The tripeptide ACV is then cyclized to isopenicillin N (IPN) by the isopenicillin N synthase PCBCC that forms the beta-lactam nucleus. Finally, the alpha-aminoadipyl side chain is exchanged for phenylacetic acid by the isopenicillin N acyltransferase PCBDE to yield penicillin in the peroxisomal matrix (By similarity).
Subcellular Localization:
Cytoplasm, cytosol. Vacuole membrane; Peripheral membrane protein. Note=Loosely attached to the vacuoles.
Domain:
NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for L- to D-amino acid conversion) are present within the NRP synthetase. GliP has the following architecture: A-T-C-A-T-C-A-T-E-TE.
Sequence:
MALEQWKTTVQSVSERCDLSGLSQHPTDYQLASTGVKGAGGSSIEERSAIVSDELFSSLRDVCSQRQLDPRSLMLFSVHQMLKRFGNGSHTVVASLVTSSEGCPSTSAWRAIPSVIHHIEGGDNNNTVASAVEQAANLLNSEGSGQDLLIPIGLTELVKSELIDLLVIFDDETNNIRLPQDFPLILRIHQRQDHWQLSVRYPSPLFDTMVIDSFLSALHNLLSAVTKPSQLVRDIELLPEYQVAQLEKWNNTDGDYPTEKRLHHLFEEAAVRRPQHVALICGDKRITYEELNAMANRLAHHLVSSGIQTEQLVGLFLDKTELMIATILGIWKSGAAHVPIDPGYPDERVKFVLNDTKAQVVIASQRHVDRLRAEAVGGQHLRIIGLESLFDNLAQQTQHSPETSGNLTHLPLNSKQLAYVTYTSGTTGFPKGIYKEHTSVVNSITDLSARYGVAGEDDEVILVFSAYVFEPFVRQMLMALTTGNSLAIISDEDKFDPDTLIPFIQKHKVTYIHATSSVLQEYDFGSCPSLKRMILVGENLTEPRYEALRQRFKSRILNEYGFTESAFVTALNIFEPTSQRKDMSLGRPVRNVKCYILDANLKRVPIGVTGELHIGGLGISRGYMNREELTRQKFLPNPYQTDKERQRGVNSTMYKTGDLARWLPSGEVEYLGRADFQIKLRGIRIEPGEIESTLAMYPGIRASIVVSKKLLSQGQETIQDHLVGYYVCDEGHIPEGDLLSFLEKKLPRYMVPTRLVQLAQIPTNINGKADLRALPAVEVAVAPTHKQDGERGNQLESDLAAIWGNILSVPAQDIGSESNFFRLGGHSIACIQLIARVRQQLGQGITLEEVFQTKTLRAMAALLSEKYTKASNGTNGVTNGTAHVNGHAANGHVSDSYVASSLQQGFVYHSLKNELSEAYTMQSMIHYGVPLKRDIYQAAWQRVQGEHPALRLRFTWEAEVMQIVDPKSELDWRVVDWTDVSSREKQLVALEQLQTEDLAKVYHLDKGPLMRLYLILLPDSKYSCLFSCHHAILDGWSLPLLFNNVHQAYLDLVEGTASPVEQDATYLLGQQYLQSHRDDHLDFWAEQIGRIEERCDMNALLNEASRYKVPLADYDQVREQRQQTISLPWNNSMDAGVREELSSRGITLHSILQTVWHLVLHSYGGGTHTITGTTISGRHLPVPGIERSVGLFINTLPMIFDHTVCQDMTALEAIEHVQGQVNAMNSRGNVELGRMSKNDLKHGLFDTLFVLENYPNLDTEQREKHEEKLKFTIKGGTEKLSYPLAVIAQEDGDSGCSFTLCYAGELFTDESIQALLDTVRDTLSDILGNIHAPIRNMEYLSSNQTAQLDKWNATAFEYPNTTLHAMFESEAQQKPDKVAVVYEDIRLTYRELNSRANALAFYLLSQAAIQPNKLVGLIMDKSEHMITSILAVWKTGGAYVPIDPRYPDQRIQYILEDTAALAVITDSPHIDRLRSITNNRLPVIQSDFALQLPPSPVHPVSNCKPSDLAYIMYTSGTTGNPKGVMVEHHGVVNLCVSLCRLFGLRNTDDEVILSFSNYVFDHFVEQMTDALLNGQTLVVLNDEMRGDKERLYRYIETNRVTYLSGTPSVISMYEFDRFRDHLRRVDCVGEAFSEPVFDKIRETFPGLIINGYGPTEVSITTHKRPYPFPERRTDKSIGCQLDNSTSYVLNDDMKRVPIGAVGELYLGGDGVARGYHNRPDLTADRFPANPFQTEQERLEGRNARLYKTGDLVRWIHNANGDGEIEYLGRNDFQVKIRGQRIELGEIEAVLSSYPGIKQSVVLAKDRKNDGQKYLVGYFVSSAGSLSAQAIRRFMLTSLPDYMVPAQLVPIAKFPVTVSGKLDAKALPVPDDTVEDDIVPPRTEVERILAGIWSELLEIPVDRISIYSDFFSLGGDSLKSTKLSFAATRALGVAVSVRNLFSHPTIEALSQWIIRGSNEVKDVAVVKGGASLDIPLSPAQERLMFIHEFGHSGEDTGAYNVPLQLQLHHDVCLESLEKALRDVVSRHEALRTLITRTQKSSVHCQKILDAEEAQKLFSVDVLRLTSETEMQGRMAESTAHAFKLDEELPIHVRLYQVVRDGRTLSFASIVCHHLAFDAWSWDVFQRDLDAFYAVHTKHKAAANLPTLRVQYKEYAIEHRRALRAEQHRVLADYWLRKLSDMEASYLVPDRPRPAQFDYTGNDLQFSTTPETTAQLKELAKREGSSLYTVVAAAYFLLLYVYTNQRDITIGIPVAHRNHPDFESVVGFFVNLLPLRVNVSQSDIHGLIQAVQKELVDAQIHQDLPFQEITKLLHVQHDPSRHPLLQAVFNWENVPANVHEEQLLQEYKPPSPLPSAAKFDLNVTVKESVNSLNVNFNYPTSLFEEETVQGFMETFHLLLRQLAHNKASTSLSKLSVEDGVLNPEPTNLQPSSRDSGNSLHGLFEDIVASTPDRIAIADGTRSLSYSELNERANQLVHLIISSASIVADDRIALLLDKSIDMVIALLAVWKAGAAYVPLDPTYPSQRTELILEESSARTLITTRKHTPRGGTVANVPSVVLDSPETLACLNQQSKENPTTSTQKPSDLAYVIFTSGTTGKPKGVLVEHQSVVQLRNSLIERYFGETNGSHAVLFLSNYVFDFSLEQLCLSVLGGNKLIIPPEEGLTHEAFYDIGRREKLSYLSGTPSVLQQIELSRLPHLHMVTAAGEEFHASQFEKMRSQFAGQINNAYGITETTVYNIITTFKGDAPFTKALCHGIPGSHVYVLNDRLQRVPFNAVGELYLGGDCLARGYLNQDALTNERFIPNPFYEPKQASDSRPQRLYKTGDLVRFRGPHHLEYLGRKDQQVKLRGFRIELSEVRDAVLAISAVKEAAVIPKYDEDGSDSRRVSAIVCYYTLNAGTVCEASSIRDHLHANLPPYMVPSQIHQLEGSLPVTVNGKLDLNRLSTTQVSQPELYTAPRNSTEETLCQLWASLLGVDHCGIDDDLFARGGDSISSLRLVGDIYRALGRKVTVKDIYLHRSVRALSENVLTDQKDKGTLPASPPLQRAEQGQVEGDAPLLPIQDWFLSKPLDNPAYWNHCFTIRTGALSVEGLRGALKLLQERHDVLRLRLQRRDEGRHVQTFARDCAQPRLTVLDRRSFEDAEDVQEALCEIQSHFDLENGPLYTVAYIHGYEDGSARVWFACHHVMVDTVSWNIILQDLQALYHGDSLGPKSSSVQQWSLAVSDYKMPLSERAHWNVLRKTVAQSFETLPICMGGVLQCQEKFSRETTTALLSKACPALDSGMHEILLMAVGSALQKAAGDVPQVVTIEGHGREDTIDATLDVSRTVGWFTSMYPFEIPKVTDPAQGVVDVKEAMRRVPNRGVGYGPAYGYGGSCLPAVSFNYLGRLDQASSGAQRDWTLVMDEDEYPVGLCTSAEDSGRSSSMVDFTFSISGGQLVMDMSSSWGHGARNEFVRTVRNTLDDLIKTTSSRDFSAPLPPSDQESSFTPYFVFEEGERHGAPLFLLPPGEGGAESYFHNIVKGLPNRNLVVFNNHYREEKTLRTIEALAEYYLSHIRSIQPEGPYHILGWSFGGILGLEAAKRLTGEGHKIATLALIDPYFDIPSASKAIGQPDDACVLDPIYHVYHPSPESFRTVSSLTNHIALFKATETNDQHGNATQQALYEWFATCPLNNLDKFLAADTIKVVPLEGTHFTWVHHPEQVRSMCTMLDEWLG