The following text describes a protein:
Function:
Component of the perinuclear meiotic nuage, a germline-specific subcellular membraneless ribonucleoprotein compartment involved in production of transposable element-repressing Piwi-interacting RNA (piRNA)-induced silencing complexes (piRISCs), which are essential for maintaining germline integrity during oogenesis. Acts via the Piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Piwi protein that directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates predominantly with sense piRNAs that contain adenine at nucleotide 10, but shows no preference for uridine at the 5' end. Shows RNA cleavage or slicer activity. Together with Piwi protein aub recruited to subregions of the perinuclear nuage by krimp, which coordinates their activity in the ping-pong amplification step of secondary piRNA biogenesis. Krimp recruits piRNA bound aub and unbound AGO3, bringing them into close proximity to facilitate the loading onto AGO3 of freshly cut piRNAs generated by aub cleavage of target sequences; krimp recognizes the piRNA loading state of the Piwi proteins via symmetrically dimethylated arginine modification in their N-terminus. Important for asymmetric ping-pong amplification to bias production towards antisense piRNAs capable of silencing transposable elements. In testis, associates with Su(Ste) and AT-chX-1 piRNAs mostly produced from antisense precursors. In the germline, acts to amplify pools of antisense piRNAs, among others Su(Ste), AT-chX-1 and roo, and to limit sense piRNA accumulation. Forms a complex with smg, twin, aub and specific piRNAs that targets nos mRNA (and probably other maternal mRNAS) for deadenylation promoting its decay during early embryogenesis. Involved in transposon silencing in the adult brain.
Subcellular Localization:
Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, Cytoplasmic ribonucleoprotein granule. Note=Component of the perinuclear meiotic nuage (also known as germline granule or P granule), a germline-specific membraneless ribonucleoprotein biocondensate involved in post-transcriptional regulation of transposons and mRNAs. Adopts a granular distribution within the nuage. Localization to the nuage is dependent on aub and krimp but not tud. piRNA binding increases mobility within the nuage. Also found in prominent but discrete foci in the germarium. Unlike other nuage components, does not accumulate at the posterior pole in oocytes and early embryos. In the cytoplasm of syncytial embryos, accumulates in discrete foci.
Sequence:
MSGRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESSGERLLSSHNIETDLITTLQHVNISVGRGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGIPAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGNRIKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL