page_title: Q-FISH
section_title: General method/protocol for cultured cells
hierarchical_section_title: Q-FISH / General method/protocol for cultured cells
caption_attribution_description: English: General workflow for Q-FISH with cultured cells.
context_page_description: Quantitative Fluorescent in situ hybridization is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled synthetic DNA mimics called peptide nucleic acid oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software. Q-FISH is most commonly used to study telomere length, which in vertebrates are repetitive hexameric sequences located at the distal end of chromosomes. Telomeres are necessary at chromosome ends to prevent DNA-damage responses as well as genome instability. To this day, the Q-FISH method continues to be utilized in the field of telomere research.
caption: Basic workflow of Q-FISH.
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