page_title: Synchronous coefficient of drag alteration
section_title: Sequence-specific
hierarchical_section_title: Synchronous coefficient of drag alteration / Applications / Sequence-specific
caption_attribution_description: English: Mutant DNA (green) is being separated from Wild Type DNA (red) during Sequence Specific SCODA.
context_page_description: Synchronous coefficient of drag alteration is a biotechnology method for purifying, separating and/or concentrating bio-molecules. SCODA has the ability to separate molecules whose mobility can be altered in sync with a driving field. This technique has been primarily used for concentrating and purifying DNA, where DNA mobility changes with an applied electrophoretic field. Electrophoretic SCODA has also been demonstrated with RNA and proteins.
context_section_description: The non-linear mobility of DNA in gel can be further controlled by embedding in the SCODA gel DNA oligonucleotides complementary to DNA fragments in the sample. This then results in highly specific non-linear velocities for the sample DNA that matches the gel-embedded DNA. This artificial specific non-linearity is then used to selectively concentrate only sequences of interest while rejecting all other DNA sequences in the sample. Over 1,000,000-fold enrichment of single nucleotide variants over wild-type have been demonstrated.
  An application of this technique is the detection of rare DNA tumour-derived DNA (ctDNA) from blood samples.
caption: Mutant DNA (green) is being separated from wild type DNA (red) during sequence specific SCODA.
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