page_title: Crossover junction endodeoxyribonuclease
section_title: Structure
hierarchical_section_title: Crossover junction endodeoxyribonuclease / Structure
caption_attribution_description: English: Descriotion
context_page_description: Crossover junction endodeoxyribonuclease, also known as Holliday junction resolvase, Holliday junction endonuclease, Holliday junction-cleaving endonuclease, Holliday junction-resolving endoribonuclease, crossover junction endoribonuclease, and cruciform-cutting endonuclease, is an enzyme involved in DNA repair and homologous recombination. Specifically, it performs endonucleolytic cleavage that results in single-stranded crossover between two homologous DNA molecules at the Holliday junction to produce recombinant DNA products for chromosomal segregation. This process is known as Holliday junction resolution.
context_section_description: E. coli RuvC, a Crossover junction endodeoxyribonuclease, is a small protein of about 20 kD, and its active form is a dimer that requires and binds a magnesium ion [1]. RuvC is a 3-layer alpha-beta sandwich with a beta-sheet between 5 alpha-helices
  .  The enzyme contains two binding channels that contact the backbones of the Holliday junction over seven nucleotides. A Holliday junction resolvase enzyme has also been identified in archaea in Pyrococcus furiosus cells - it is encoded by a gene called hjc and is composed of 123 amino acids 
  .
  A figure of Thermus thermophilus RuvC in complex with a Holliday junction is shown below.
caption: Archaea crossover junction endodeoxyribonuclease in complex with Holliday Junction DNA. Generated with 4LD0.pdb.[6]
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