page_title: Point spread function
hierarchical_section_title: Point spread function
caption_attribution_description: Convolution illustrated: longitudinal (XZ) central slice of a 3D image acquired by a fluorescence microscope.
context_page_description: The point spread function describes the response of an imaging system to a point source or point object. A more general term for the PSF is a system's impulse response, the PSF being the impulse response of a focused optical system. The PSF in many contexts can be thought of as the extended blob in an image that represents a single point object. In functional terms, it is the spatial domain version of the optical transfer function of the imaging system. It is a useful concept in Fourier optics, astronomical imaging, medical imaging, electron microscopy and other imaging techniques such as 3D microscopy and fluorescence microscopy.
  The degree of spreading of the point object is a measure for the quality of an imaging system. In non-coherent imaging systems, such as fluorescent microscopes, telescopes or optical microscopes, the image formation process is linear in the image intensity and described by linear system theory. This means that when two objects A and B are imaged simultaneously, the resulting image is equal to the sum of the independently imaged objects.
context_section_description: The point spread function (PSF) describes the response of an imaging system to a point source or point object. A more general term for the PSF is a system's impulse response, the PSF being the impulse response of a focused optical system. The PSF in many contexts can be thought of as the extended blob in an image that represents a single point object. In functional terms, it is the spatial domain version of the optical transfer function of the imaging system. It is a useful concept in Fourier optics, astronomical imaging, medical imaging, electron microscopy and other imaging techniques such as 3D microscopy (like in confocal laser scanning microscopy) and fluorescence microscopy. 
  The degree of spreading (blurring) of the point object is a measure for the quality of an imaging system. In non-coherent imaging systems, such as fluorescent microscopes, telescopes or optical microscopes, the image formation process is linear in the image intensity and described by linear system theory. This means that when two objects A and B are imaged simultaneously, the resulting image is equal to the sum of the independently imaged objects. In other words: the imaging of A is unaffected by the imaging of B and vice versa, owing to the non-interacting property of photons. In space-invariant system, i.e. the PSF is the same everywhere in the imaging space, the image of a complex object is then the convolution of the true object and the PSF.
caption: Image formation in a confocal microscope: central longitudinal (XZ) slice. The 3D acquired distribution arises from the convolution of the real light sources with the PSF.
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