page_title: Replication terminator Tus family
hierarchical_section_title: Replication terminator Tus family
caption_attribution_description: English: Tus-Ter complex; from protein data bank file
context_page_description: Tus, also known as terminus utilization substance, is a protein that binds to terminator sequences and acts as a counter-helicase when it comes in contact with an advancing helicase. The bound Tus protein effectively halts DNA polymerase movement. Tus helps end DNA replication in prokaryotes.
  In E. coli, Tus binds to 10 closely related sites encoded in the chromosome. These sites bind 23 base-pairs. The 10 sites are called Ter sites, and are designated TerA, TerB, ..., TerJ. These binding sites are asymmetric, such that when a Tus-Ter complex is encountered by a replication fork from one direction, the complex is dissociated and replication continues. But when encountered from the other direction, the Tus-Ter complex provides a much larger kinetic barrier and halts replication. The multiple Ter sites in the chromosome are oriented such that the two oppositely moving replication forks are both stalled in the desired termination region.
  Bacillus subtilis utilize replication terminator protein instead of Tus.
context_section_description: Tus, also known as terminus utilization substance, is a protein that binds to terminator sequences and acts as a counter-helicase when it comes in contact with an advancing helicase. The bound Tus protein effectively halts DNA polymerase movement. Tus helps end DNA replication in prokaryotes.
  In E. coli, Tus binds to 10 closely related sites encoded in the chromosome. These sites bind 23 base-pairs. The 10 sites are called Ter sites, and are designated TerA, TerB, ..., TerJ.  These binding sites are asymmetric, such that when a Tus-Ter complex (Tus protein bound to a Ter site) is encountered by a replication fork from one direction, the complex is dissociated and replication continues (permissive). But when encountered from the other direction, the Tus-Ter complex provides a much larger kinetic barrier and halts replication (non-permissive). The multiple Ter sites in the chromosome are oriented such that the two oppositely moving replication forks are both stalled in the desired termination region.
  Bacillus subtilis utilize replication terminator protein (RTP) instead of Tus.
caption: Representation of the x-ray crystal structure of Tus-Ter protein-DNA complex. (Jmol rendering of coordinates from.[1]  The DNA strands are shown in pink and green.)
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