page_title: Cfr10I/Bse634I
hierarchical_section_title: Cfr10I/Bse634I
caption_attribution_description: English: Cartoon representation of the molecular structure of protein registered with 1cfr code. Deutsch: Grafik des Molekularstruktur von jenem Protein, das mit 1cfr code registriert ist. Plattdüütsch: Grafik, de de Molekularstruktur von dat Protein wiest, dat mit’n Kood 1cfr registreert is.
context_page_description: In molecular biology, the Cfr10I/Bse634I family of restriction endonucleases includes the type II restriction endonucleases Cfr10I and Bse634I. They exhibit a conserved tetrameric architecture that is of functional importance, wherein two dimers are arranged, back-to-back, with their putative DNA-binding clefts facing opposite directions. These clefts are formed between two monomers that interact, mainly via hydrophobic interactions supported by a few hydrogen bonds, to form a U-shaped dimer. Each monomer is folded to form a compact alpha-beta structure, whose core is made up of a five-stranded mixed beta-sheet. The monomer may be split into separate N-terminal and C-terminal subdomains at a hinge located in helix alpha3. Both Cfr10I and Bse634I recognise the double-stranded sequence RCCGGY and cleave after the purine R.
  Recognition sequence Cut
  5' RCCGGY 5' ---R CCGGY--- 3'
  3' YGGCCR 3' ---YGGCC R--- 5'
context_section_description: In molecular biology, the Cfr10I/Bse634I family of restriction endonucleases includes the type II restriction endonucleases Cfr10I and Bse634I. They exhibit a conserved tetrameric architecture that is of functional importance, wherein two dimers are arranged, back-to-back, with their putative DNA-binding clefts facing opposite directions. These clefts are formed between two monomers that interact, mainly via hydrophobic interactions supported by a few hydrogen bonds, to form a U-shaped dimer. Each monomer is folded to form a compact alpha-beta structure, whose core is made up of a five-stranded mixed beta-sheet. The monomer may be split into separate N-terminal and C-terminal subdomains at a hinge located in helix alpha3. Both Cfr10I and Bse634I recognise the double-stranded sequence RCCGGY and cleave after the purine R.
  Recognition sequence     Cut
  5' RCCGGY                5' ---R   CCGGY--- 3'
  3' YGGCCR 	         3' ---YGGCC   R--- 5'
caption: crystal structure of citrobacter freundii restriction endonuclease cfr10i at 2.15 angstroms resolution.
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