page_title: Digital transcriptome subtraction
section_title: Method
hierarchical_section_title: Digital transcriptome subtraction / Method
caption_attribution_description: English: Breakdown of raw transcripts obtained from sequencing human tissue-derived libraries.
context_page_description: Digital transcriptome subtraction is a bioinformatics method to detect the presence of novel pathogen transcripts through computational removal of the host sequences. DTS is the direct in silico analogue of the wet-lab approach Representational Difference Analysis, and is made possible by unbiased high-throughput sequencing and the availability of a high-quality, annotated reference genome of the host. The method specifically examines the etiological agent of infectious diseases and is best known for discovering Merkel cell polymavirus, the suspect causative agent in Merkel cell carcinoma.
caption: Fig. 2. Raw transcript breakdown from sequencing 20,000 clones derived from virus-infected human tissues. Viral transcripts were present at 0.03% of the total sequence reads.[3]
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