page_title: 3D cell culturing by magnetic levitation
section_title: Organized co-culturing to create in vivo-like tissue
hierarchical_section_title: 3D cell culturing by magnetic levitation / Applications / Organized co-culturing to create in vivo-like tissue
caption_attribution_description: English: IHC reveals epithelial markers on A549 after culturing in 3D with the MLM as compared with 2D culturing
context_page_description: 3D cell culture by the magnetic levitation method is the application of growing 3D tissue by inducing cells treated with magnetic nanoparticle assemblies in spatially varying magnetic fields using neodymium magnetic drivers and promoting cell to cell interactions by levitating the cells up to the air/liquid interface of a standard petri dish. The magnetic nanoparticle assemblies consist of magnetic iron oxide nanoparticles, gold nanoparticles, and the polymer polylysine. 3D cell culturing is scalable, with the capability for culturing 500 cells to millions of cells or from single dish to high-throughput low volume systems. Once magnetized cultures are generated, they can also be used as the building block material, or the "ink", for the magnetic 3D bioprinting process.
context_section_description: Using the MagPen™ (a Nano3D Biosciences, Inc. product), organized 3D co-cultures similar to native tissue architecture can be rapidly created. Endothelial cells (PEC), smooth muscle cells (SMC), fibroblasts (PF), and epithelial cells (EpiC) cultured with the Bio-Assembler™ can be sequentially layered in a drag-and-drop manner to create bronchioles that maintain phenotype and induce extracellular matrix formation.
caption: IHC reveals epithelial markers on A549 after culturing in 3D with the MLM
exif: {"Image PhotometricInterpretation": "2", "Image Orientation": "Horizontal (normal)", "Image XResolution": "72", "Image YResolution": "72", "Image ResolutionUnit": "Pixels/Inch", "Image ExifOffset": "102", "EXIF ExifImageWidth": "471", "EXIF ExifImageLength": "147"}