page_title: Minusheet perfusion culture system
section_title: Modulation of oxygen content
hierarchical_section_title: Minusheet perfusion culture system / Modulation of oxygen content
caption_attribution_description: English: a. Gas exchange module b. Gas expander module
context_page_description: Minusheet perfusion culture system is used for advanced cell culture experiments in combination with adherent cells and to generate specialized tissues in combination with selected biomaterials, special tissue carriers and compatible perfusion culture containers.
  The technical development of the Minusheet perfusion culture system was driven by the idea to create under in vitro conditions an environment resembling as near as possible the situation of specialized tissues found within the organism. Basis of this invention is therefore individually selected biomaterials for optimal cell adhesion mounted in Minusheet tissue carriers. Moreover, to always offer fresh nutrition including respiratory gas and to simulate a tissue-specific fluid environment, the tissue carriers can be inserted into compatible perfusion culture containers. As a result, a variety of publications illustrates that tissues generated by this innovative approach exhibit an excellent and stable quality. Thus, on the one hand the system provides a highly adaptable basis for the culture of adherent cells and the generation of specialized tissues.
context_section_description: It has been shown that growing cells and tissues have very individual oxygen requirements. Due to this reason it is important that the content of oxygen can be adapted in individual perfusion culture experiments. The technical solution is a gas exchanger module containing a gas inlet and outlet (Fig. 6a). Further a spiral with a long thin-walled silicon tube for medium transport is mounted inside the module. Since the tube is highly gas-permeable, it guarantees optimal diffusion of gases between culture medium and internal atmosphere of the gas exchange module. In consequence, the desired gas atmosphere can be adjusted by a constant flow of a specific gas mixture through the module. This way the content of oxygen or any other gases can be modulated in the medium by diffusion. Applying this simple protocol it became possible to decrease the oxygen partial pressure within the transported medium during long term culture experiments under absolutely sterile conditions [7].
caption: Figure 6: Gas exchange and gas expander module for perfusion culture. (a) A gas exchange module is used, when adaption of respiratory gas in perfusion culture has to be performed. By a gas inlet and outlet the desired gas composition is transported to a spiral of silicone tube containing the culture medium. Between the wall of the silicone tube gas is exchanged. (b) A gas expander module is used for the elimination of gas bubbles during transport of culture medium. Medium with gas bubbles is entering at the inlet and has to pass a barrier. Here the medium expands and bubbles are separated. Finally medium is leaving the container bubble-free but gas-saturated.
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