page_title: Epitranscriptomic sequencing
section_title: Methods for Inosine Profiling
hierarchical_section_title: Epitranscriptomic sequencing / Methods for Inosine Profiling
caption_attribution_description: English: Inosine base pairs differently to adenosine (from which it is derived). Inosine pairs with cytidine while adenosine pairs with uracil. Therefore a cDNA library can be constructed and the RNA products compared against the genome.
context_page_description: In epitranscriptomic sequencing, most methods focus on either enrichment and purification of the modified RNA molecules before running on the RNA sequencer, or improving or modifying bioinformatics analysis pipelines to call the modification peaks. Most methods have been adapted and optimized for mRNA molecules, except for modified bisulfite sequencing for profiling 5-methylcytidine which was optimized for tRNAs and rRNAs.
  There are six major classes of chemical modifications found in RNA molecules: N⁶-methyladenosine, N6,2'-O-dimethyladenosine, 5-methylcytidine, 5-hydroxylmethylcytidine, inosine, and pseudouridine. Various sequencing methods have been developed to profile each type of modification. The scale, resolution, sensitivity, and limitations associated with each method and the corresponding bioinformatics tools used will be discussed.
context_section_description: Inosine is created enzymatically when an adenosine residue is modified.
caption: Method of inosine identification: Inosine base pairs with cytidine while adenosine pairs with uracil.
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