page_title: EcoRV
hierarchical_section_title: EcoRV
caption_attribution_description: Schematic representation of the function of restriction endonuclease EcoRV. This protein loosely binds DNA and scans for its cognate sequence. Once found, EcoRV kinks the DNA in a 50° angle and cleaves at the cognate sequence.
context_page_description: EcoRV is a type II restriction endonuclease isolated from certain strains of Escherichia coli. It has the alternative name Eco32I.
  In molecular biology, it is a commonly used restriction enzyme. It creates blunt ends. The enzyme recognizes the palindromic 6-base DNA sequence 5'-GAT|ATC-3' and makes a cut at the vertical line. The complementary sequence is then 3'-CTA|TAG-5'. The ends are blunt and can be ligated into a blunt cloning site easily but with lower efficiency than sticky ends.
context_section_description: EcoRV (pronounced "eco R five") is a type II restriction endonuclease isolated from certain strains of Escherichia coli. It has the alternative name Eco32I.
  In molecular biology, it is a commonly used restriction enzyme. It creates blunt ends. The enzyme recognizes the palindromic 6-base DNA sequence 5'-GAT|ATC-3' and makes a cut at the vertical line. The complementary sequence is then 3'-CTA|TAG-5'. The ends are blunt and can be ligated into a blunt cloning site easily but with lower efficiency than sticky ends.
caption: EcoRV cleaving DNA. The protein loosely binds DNA and scans for its recognition sequence. Once found, EcoRV kinks the DNA in a 50° angle and cleaves at the cognate sequence.
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