page_title: Control of chromosome duplication
section_title: The pre-replication complex
hierarchical_section_title: Control of chromosome duplication / The pre-replication complex
caption_attribution_description: English: Pre-Replcative Complex Assembly in Yeast
context_page_description: In cell biology, eukaryotes possess a regulatory system that ensures that DNA replication occurs only once per cell cycle.
  A key feature of the DNA replication mechanism in eukaryotes is that it is designed to replicate relatively large genomes rapidly and with high fidelity. Replication is initiated at multiple origins of replication on multiple chromosomes simultaneously so that the duration of S phase is not limited by the total amount of DNA. This flexibility in genome size comes at a cost: there has to be a high-fidelity control system that coordinates multiple replication origins so that they are activated only once during each S phase. If this were not the case, daughter cells might inherit an excessive amount of any DNA sequence, which could lead to many harmful effects.
context_section_description: Before DNA replication can start, the pre-replicative complex assembles at origins to load helicase onto DNA. The complex assembles in late mitosis and early G1.  Assembly of these pre-replicative complexes (pre-RCs) is regulated in a manner that coordinates DNA replication with the cell cycle.
caption: Pre-RC assembly involves the assembly of the ORC subunits, Cdc6 and Cdt1 and the Mcm2-7 complex
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