page_title: Metarhizium granulomatis
section_title: Growth and morphology
hierarchical_section_title: Metarhizium granulomatis / Growth and morphology
caption_attribution_description: English: UAMH 11177 Metarhizium granulomatis in phase contrast microscopy showing yeast-like cells grown in slide culture on PDA at room temperature
context_page_description: Metarhizium granulomatis is a fungus in the family Clavicipitaceae associated with systemic mycosis in veiled chameleons. The genus Metarhizium is known to infect arthropods, and collectively are referred to green-spored asexual pathogenic fungi. This species grows near the roots of plants and has been reported as an agent of disease in captive veiled chameleons. The etymology of the species epithet, "granulomatis" refers to the ability of the fungus to cause granulomatous disease in susceptible reptiles.
context_section_description: Colonies of M. granulomatis differ in morphology depending on the growth medium used. Colonies on Potato dextrose agar (PDA) after 3 days incubation at 30 °C (86 °F) are white in colour and reach 3 mm in diameter. Cultures developed on Sabouraud dextrose agar (SDA) are similar in appearance although they grow more slowly on this medium. After 10 days, colonies on PDA reached a diameter of 27 mm and produced a diffusible brownish-grey pigment. Colonies the SDA are 12 mm in diameter and grey-ochre in colour. The hyphae of M. granulomatis are septate and bear phialides that have single apical openings (monophialides) resembling a wine bottle. The conidia produced are in short, curved, chains, appearing glassy and smooth. Conidia are spherical-to-oval and 3.3-3.8 μm in length by 1.2-1.6 μm in width. The fungus produces a yeast-like stage in vitro on PDA after 3 days incubation at 35°C.
caption: UAMH 11177 Metarhizium granulomatis in phase contrast microscopy showing yeast-like cells grown in slide culture on PDA at room temperature
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