page_title: Glowmatography
section_title: Experiment
hierarchical_section_title: Glowmatography / Experiment
caption_attribution_description: English: Demonstration of glowmatography with purple dye 1: Solvant travels up the chalk. 2: Separation starts as the red dye is attracted to the mobile phase and starts traveling up while the blue dye is attracted to the stationary phase and stays relatively still. 3: Complete separation occurs as time passes.
context_page_description: Glowmatography is a laboratory technique for the extraction of dyes present in solutions contained in glow sticks. The chemical components of such solutions can be chromatographically separated into polar and nonpolar components. Developed as a laboratory class experiment, it can be used to demonstrate chemistry concepts of polarity, chemical kinetics, and chemiluminescence.
context_section_description: This experiment can be conducted with glow sticks, chalks, and solutions of acetone or isopropyl alcohol. 
  Drops of glowing fluid from a glow stick is added to a chalk so that a band is created halfway through it. The chalk is then placed vertically into a beaker filled with a small amount of acetone or alcohol - ensuring the surface of the solvent is below the dye band. The liquid is then allowed to travel up the chalk; polar dyes would tend to stick to the chalk and not travel significantly while non-polar dyes would travel up with the solvent. Once it travels almost to the top of the chalk, it is removed from the beaker. The chalk chromatogram, with separation of colours, can then be observed in a dark room.
  Additionally, this glomatographic experiment can be done using other materials. For instance, silica gel can be used as the stationary phase together with a solution of nonpolar hexanes acting as the mobile phase. The polar components would be attracted to the polar silanol (Si-OH) groups on the surface of the silica gel, and the nonpolar components would travel further with the hexanes. Further, dyes in glow sticks can also be extracted using liquid carbon dioxide (CO₂) as an environmentally friendly or green solvent. In this case, non polar dyes would dissolve in the liquid CO₂ and other dyes would be attracted to cotton.
caption: Diagram of glowmatography with purple dye. 1: Solvent travels up the chalk. 2: Separation starts as the red dye is attracted to the mobile phase and starts traveling up while the blue dye is attracted to the stationary phase and stays relatively still. 3: Complete separation occurs as time passes.
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