page_title: Flow-induced dispersion analysis
hierarchical_section_title: Flow-induced dispersion analysis
caption_attribution_description: English: Explanatory figure of the FIDA principle
context_page_description: Flow-induced dispersion analysis is an immobilization-free technology used for characterization and quantification of biomolecular interaction and protein concentration under native conditions. In the FIDA assay, the size of a ligand with affinity to the target analyte is measured. When the indicator interacts with the analyte the apparent size increases and this change in size can be used to determine the analyte concentration and interaction. Additionally, the hydrodynamic radius of the analyte-indicator complex is obtained. A FIDA assay is typically completed in minutes and only requires a modest sample consumption of a few µl.
context_section_description: Flow-induced dispersion analysis (FIDA) is an immobilization-free technology used for characterization and quantification of biomolecular interaction and protein concentration under native conditions. In the FIDA assay, the size of a ligand (indicator) with affinity to the target analyte is measured. When the indicator interacts with the analyte the apparent size increases and this change in size can be used to determine the analyte concentration and interaction. Additionally, the hydrodynamic radius of the analyte-indicator complex is obtained. A FIDA assay is typically completed in minutes and only requires a modest sample consumption of a few µl.
caption: The FIDA principle: A narrow indicator zone is introduced into a capillary under hydrodynamic flow. When the indicator is not bound, a narrow peak is observed at the detector. However, when the indicator is bound by the target analyte, the apparent size increases and a broader peak is observed. This change in size can be used for determine the analyte concentration and interaction[1] - Published by The Royal Society of Chemistry.
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