page_title: Mitotic cell rounding
hierarchical_section_title: Mitotic cell rounding
caption_attribution_description: English: Example of a transgenic HeLa cell expressing H2B-GFP going from G2 through to the end of mitosis. DIC and GFP images are shown every 4 minutes. The phases and designated colour schemes are shown on the right. GFP-H2B images were used to determine the phase of mitosis according to the following criteria: prophase cells contain condensed chromosomes surrounded by an intact nuclear envelope; prometaphase starts with nuclear envelope breakdown; metaphase is when chromosomes align to form the metaphase plate; anaphase starts when the chromosomes begin to separate; telophase commences when chromosomes de-condense.
context_page_description: Mitotic cell rounding is a shape change that occurs in most animal cells that undergo mitosis. Cells abandon the spread or elongated shape characteristic of interphase and contract into a spherical morphology during mitosis. The phenomena is seen both in artificial cultures in vitro and naturally forming tissue in vivo.
context_section_description: Mitotic cell rounding is a shape change that occurs in most animal cells that undergo mitosis. Cells abandon the spread or elongated shape characteristic of interphase and contract into a spherical morphology during mitosis. The phenomena is seen both in artificial cultures in vitro and naturally forming tissue in vivo.
caption: Cell shape changes as a function of mitotic phase. Shown is an example of a HeLa cell cultured on a glass surface. For visualization of DNA and mitotic phase assignment, the cell expresses Histone H2B-GFP to provide fluorescent labeling of chromosomes. Transmitted light (DIC), fluorescence (GFP), and merged images are shown every 4 minutes as the cell transitions from G2 phase through mitosis to telophase/G1 phase.
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