page_title: FokI
hierarchical_section_title: FokI
caption_attribution_description: English: RESTRICTION ENDONUCLEASE FOKI BOUND TO DNA. PDB 1fok
context_page_description: The enzyme Fok1, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site.
  Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
context_section_description: The enzyme Fok1 (Fok-1), naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site.
  Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
caption: Restriction endonuclease Fok1 bound to DNA PDB 1fok [1]
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