page_title: Ribonuclease V1
section_title: Laboratory use
hierarchical_section_title: Ribonuclease V1 / Laboratory use
caption_attribution_description: English: X-ray structure of the tRNAPhe from yeast. Data was obtained by PDB: 1ehz&#8203; and rendered with PyMOL. violet: acceptor stem wine red: D-loop blue: anticodon loop orange: variable loop green: TPsiC-loop yellow: CCA-3' of the acceptor stem grey: anticodon Deutsch: Struktur der tRNAPhe aus Hefe. Dem Bild liegen die Daten aus PDB: 1ehz&#8203; zu Grunde, es wurde mittels PyMOL gerendert. violett: Akzeptorstamm weinrot: D-Schleife blau: Anticodonschleife orange: variable Schleife grün: TPsiC-Schleife gelb: CCA-3' des Akzeptorstammes grau: Anticodon Español: Estructura de rayos X de la tRNAPhe de la levadura. Los datos se obtuvieron por AP: 1ehz y se procesaron con PyMOL. violeta: vástago aceptor rojo vino: D-loop azul: lazo anticodón naranja: lazo variable verde: TPsiC-loop amarillo: CCA-3 'del vástago aceptor gris: anticodón
context_page_description: Ribonuclease V1 is a ribonuclease enzyme found in the venom of the Caspian cobra. It cleaves double-stranded RNA in a non-sequence-specific manner, usually requiring a substrate of at least six stacked nucleotides. Like many ribonucleases, the enzyme requires the presence of magnesium ions for activity.
context_section_description: Purified RNase V1 is a commonly used reagent in molecular biology experiments. In conjunction with other ribonucleases that cleave single-stranded RNA after specific nucleotides or sequences – such as RNase T1 and RNase I – it can be used to map internal interactions in large RNA molecules with complex secondary structure or to perform footprinting experiments on macromolecular complexes containing RNA.
  RNase V1 is the only commonly used laboratory RNase that provides positive evidence for the presence of double-stranded helical conformations in target RNA. Because RNase V1 has some activity against RNA that is base-paired but single-stranded, dual susceptibility to both RNase V1 and RNase I at a single site in a target RNA molecule provides evidence of this relatively unusual conformation found in RNA loops.
caption: The distinctive secondary structure of transfer RNA, containing a series of double helices separated by flexible loops
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