page_title: Immunocytochemistry
hierarchical_section_title: Immunocytochemistry
caption_attribution_description: English: A myenteric ganglion of a mouse with tyrosine hydroxylase (denoting sympathetic autonomic axons) labeled by immunohistochemistry (green) acquired with a laser scanning confocal microscope. Blue color is UV-range autofluorescence from postganglionic neurons. Français&#160;: Image d'un ganglion dans le plexus myentérique de la souris avec de la la tyrosine hydroxylase (indiquant les axones sympathiques autonomes ) marqués par immunohistochimie (en vert). L'image est faite sous microscope confocal à balayage laser. La couleur bleue vient d'une autofluorescence sous une certaine gamme d'UV de neurones postganglioniques, marquant peut-être de la lipofuscine accumulée lors du vieillissement des cellules
context_page_description: Immunocytochemistry is a common laboratory technique that is used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it. The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore. ICC allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC also allows researchers to determine which sub-cellular compartments are expressing the antigen.
context_section_description: Immunocytochemistry (ICC) is a common laboratory technique that is used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it. The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore. ICC allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC also allows researchers to determine which sub-cellular compartments are expressing the antigen.
caption: Immunocytochemistry labels individual proteins within cells, such as TH (green) in the axons of sympathetic autonomic neurons.
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