Document:

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                                                                   EXHIBIT 10.27

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                           SECOND AMENDED AND RESTATED
                          REGISTRATION RIGHTS AGREEMENT

                                 BY AND BETWEEN

                              SEGUE SOFTWARE, INC.

                                       AND

                                  THE INVESTORS

                                AS DEFINED HEREIN

                          DATED AS OF NOVEMBER 24, 2003

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                           SECOND AMENDED AND RESTATED
                          REGISTRATION RIGHTS AGREEMENT

     THIS SECOND AMENDED AND RESTATED REGISTRATION RIGHTS AGREEMENT (the
"Agreement") is dated as of November 24, 2003, by and among Segue Software,
Inc., a Delaware corporation (the "Company"), S-7 Associates, LLC (the "Series B
Investor"), Dr. Howard L. Morgan ("Morgan") and Isaac J. Mayer ("Mayer" and,
together with Morgan, the "Additional Investors"). The Additional Investors and
the Series B Investor are collectively referred to herein as the "Investors".

     WHEREAS, the Company and the Series B Investor entered into a registration
rights agreement (the "Original Agreement"), dated as of March 22, 2002, in
connection with the Series B Investor's purchase of 666,667 shares of Series B
Preferred Stock of the Company for an aggregate purchase price of $2,000,000;

     WHEREAS, the Company, the Series B Investor and Morgan entered into an
amended and restated registration rights agreement, dated as of October 21,
2003, amending and restating the Original Agreement (the "First Amended and
Restated Agreement"), in connection with the Series B Investor's and Morgan's
purchase of 166,667 shares of Series C Preferred Stock of the Company for an
aggregate purchase price of $500,001;

     WHEREAS, the Company and Mayer are simultaneously entering into a certain
Series C Stock Purchase Agreement, dated as of the date hereof (the "Purchase
Agreement"), whereby Mayer has agreed to purchase securities from the Company
for an aggregate purchase price of $1,000,000;

     WHEREAS, the Company, the Series B Investor and Morgan desire to amend and
restate the First Amended and Restated Agreement in connection with the
transactions contemplated by the Purchase Agreement; and

     WHEREAS, the execution of this Agreement is a condition precedent to the
purchase by Mayer of the securities under the Purchase Agreement;

     NOW, THEREFORE, in consideration of the premises, as an inducement to Mayer
to consummate the transactions contemplated by the Purchase Agreement, and for
other good and valuable consideration, the receipt and sufficiency of which is
hereby acknowledged, the Company, the Series B Investor and Morgan hereby amend
and restate the First Amended and Restated Agreement in its entirety and the
Investors and the Company agree with each other as follows:

     1.   CERTAIN DEFINITIONS.
          -------------------

     As used in this Agreement, the following terms shall have the following
respective meanings:

     "Commission" means the United States Securities and Exchange Commission, or
any other federal agency administering the Securities Act and the Exchange Act
at the time.

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     "Common Stock" means the Common Stock and any other common equity
securities issued by the Company, and any other shares of stock issued or
issuable with respect thereto (whether by way of a stock dividend or stock split
or in exchange for or upon conversion of such shares or otherwise in connection
with a combination of shares, recapitalization, merger, consolidation or other
corporate reorganization).

     "Exchange Act" means the Securities Exchange Act of 1934, as amended, or
any similar successor federal statute, and the rules and regulations of the
Commission thereunder, all as the same shall be in effect at the time.

     "Person" means an individual, a corporation, a partnership, a joint
venture, a trust, an unincorporated organization, a limited liability company or
partnership, a government and any agency or political subdivision thereof.

     "Registrable Securities" means (i) any shares of Common Stock issued or
issuable upon conversion of the Company's Series C Preferred Stock or Series B
Preferred Stock, and (ii) any other securities issued and issuable with respect
to the shares described in clause (i) above by way of a stock dividend or stock
split or in connection with a combination of shares, recapitalization, merger,
consolidation or other reorganization; provided, that any such shares shall
cease to be Registrable Securities when (i) such Registrable Securities have
been disposed of by the holder thereof pursuant to an effective registration
statement under the Securities Act, (ii) such Registrable Securities are
transferred by the holder thereof to any Person pursuant to Rule 144 (or any
successor rule or similar provision then in effect) under the Securities Act,
including a sale pursuant to the provisions of Rule 144(k), or (iii) such
Securities shall have ceased to be outstanding.

     "Securities Act" means the Securities Act of 1933, as amended, or any
similar successor federal statute, and the rules and regulations of the
Commission thereunder, all as the same shall be in effect at the time.

     All other capitalized terms not defined herein shall have the meaning set
forth in the Purchase Agreement unless otherwise indicated.

     2.   DEMAND REGISTRATION ON FORM S-3.

          (a)  At any time after the date hereof and prior to the fifth
anniversary of the date hereof, the holders of at least fifty percent (50%) of
the Registrable Securities may notify the Company that they intend to offer or
cause to be offered for public sale all or any portion of their Registrable
Securities in the manner specified in such request. Upon receipt of such
request, the Company shall promptly deliver notice of such request to all
holders of Registrable Securities, who shall then have thirty (30) days to
notify the Company in writing of their desire to be included in such
registration. If the request for registration contemplates an underwritten
public offering, the Company shall state such in the written notice and in such
event the right of any Person to participate in such registration shall be
conditioned upon such Person's participation in such underwritten public
offering and the inclusion of such Person's Registrable Securities in the
underwritten public offering to the extent provided herein. The Company will use
its reasonable efforts to effect the registration on Form S-3 (or a comparable
successor form)

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of all Registrable Securities whose holders request participation in such
registration under the Securities Act, but only to the extent provided for in
this Agreement. The Company shall not be required to effect registration
pursuant to a request under this Section 2 more than one time. Notwithstanding
anything to the contrary contained herein, the Company shall not be obligated to
file a registration statement under this Section 2: (a) if Form S-3 is not then
available for the proposed offering; (b) within ninety (90) days after the
effective date of a registration statement filed by the Company covering a firm
commitment underwritten public offering in which the holders of Registrable
Securities shall have been entitled to join pursuant to Section 4; or (c) during
a Suspension Period (as defined in Section 8(a)). A registration will not count
as a requested registration under this section 2(a) unless and until the
registration statement relating to such registration has been declared effective
by the Commission.

          (b)  If a requested registration involves an underwritten public
offering and the managing underwriter of such offering determines in good faith
that the number of securities sought to be offered should be limited due to
market conditions, then the number of securities to be included in such
underwritten public offering shall be reduced to a number deemed satisfactory by
such managing underwriter; provided, that the shares to be excluded shall be
determined in the following order of priority: (i) persons not having any
contractual or other right to include such securities in the registration
statement, (ii) securities held by any other Persons (other than the holders of
Registrable Securities) having a contractual, incidental "piggyback" right to
include such securities in the registration statement, (iii) securities to be
registered by the Company pursuant to such registration statement, (iv)
Registrable Securities of holders who did not make the original request for
registration and, if necessary, (v) Registrable Securities of holders who
requested such registration pursuant to Section 2(a). If there is a reduction of
the number of Registrable Securities pursuant to clauses (iv) or (v), such
reduction shall be made on a pro rata basis (based upon the aggregate number of
Registrable Securities held by such holders).

          (c)  With respect to a request for registration pursuant to Section
2(a) which is for an underwritten public offering, the managing underwriter
shall be chosen by the holders of a majority of the Registrable Securities to be
sold in such offering (which approval will not be unreasonably withheld or
delayed). The Company may not cause any other registration of securities for
sale for its own account (other than a registration effected solely to implement
an employee benefit plan or a transaction to which Rule 145 of the Securities
Act is applicable) to become effective within ninety (90) days following the
effective date of any registration required pursuant to this Section 2.

     3.   PIGGYBACK REGISTRATION.
          ----------------------

     If the Company at any time proposes to register any of its securities under
the Securities Act for sale to the public (except with respect to registration
statements on Forms S-4, S-8 or another form not available for registering the
Registrable Securities for sale to the public), each such time it will give
written notice at the applicable address of record to each holder of Registrable
Securities of its intention to do so. Upon the written request of any of such
holders, given within twenty (20) days after receipt by such Person of such
notice, the Company will, subject to the limits contained in this Section 3, use
its reasonable efforts to cause all such Registrable Securities of said
requesting holders to be registered under the Securities Act and

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qualified for sale under any state blue sky law, all to the extent required to
permit such sale or other disposition of said Registrable Securities; provided,
however, that if the Company is advised in writing by any managing underwriter
of the Company's securities being offered in a public offering pursuant to such
registration statement that the amount to be sold by persons other than the
Company (collectively, "Selling Stockholders") is greater than the amount which
can be offered without adversely affecting the offering, the Company may reduce
the amount offered for the accounts of Selling Stockholders (including such
holders of Registrable Securities) to a number deemed satisfactory by such
managing underwriter; and provided further, that any shares to be excluded shall
be determined in the following order of priority: (i) securities held by any
Persons not having any such contractual, incidental registration rights, (ii)
securities held by any Persons having contractual, incidental registration
rights pursuant to an agreement which is not this Agreement and (iii) the
Registrable Securities sought to be included by the holders thereof as
determined on a pro rata basis (based upon the aggregate number of Registrable
Securities held by such holders).

     4.   REGISTRATION PROCEDURES.

     If and whenever the Company is required by the provisions of this Agreement
to use its reasonable efforts to promptly effect the registration of any of its
securities under the Securities Act, the Company will:

          (a)  use its reasonable efforts to prepare and file with the
Commission a registration statement on the appropriate form under the Securities
Act with respect to such securities, which form shall comply as to form in all
material respects with the requirements of the applicable form and include all
financial statements required by the Commission to be filed therewith, and use
its reasonable efforts to cause such registration statement to become and remain
effective for 90 days or until the completion of the proposed offering,
whichever is earlier; provided, that such 90-day period shall be extended for a
period of time equal to the period the selling holders refrain from selling
Registrable Securities pursuant to Section 8.

          (b)  use its reasonable best efforts to prepare and file with the
Commission such amendments and supplements to such registration statement and
the prospectus used in connection therewith as may be necessary to keep such
registration statement effective for the period as specified in Section 4(b) and
to comply with the provisions of the Securities Act with respect to the sale or
other disposition of all securities covered by such registration statement
whenever the seller or sellers of such securities shall desire to sell or
otherwise dispose of the same, but only to the extent provided in this
Agreement;

          (c)  furnish to each selling holder and the underwriters, if any, such
number of copies of such registration statement, any amendments thereto, any
documents incorporated by reference therein, the prospectus, including a
preliminary prospectus, in conformity with the requirements of the Securities
Act, and such other documents as such selling holder may reasonably request in
order to facilitate the public sale or other disposition of the securities owned
by such selling holder;

          (d)  use its reasonable efforts to register or qualify the securities
covered by such registration statement under such other securities or state blue
sky laws of such jurisdictions

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as each selling holder shall request, and to do any and all other acts and
things which may be necessary under such securities or blue sky laws to enable
such selling holder to consummate the public sale or other disposition in such
jurisdictions of the securities owned by such selling holder, except that the
Company shall not for any such purpose be required to qualify to do business as
a foreign corporation in any jurisdiction wherein it is not so qualified;

          (e)  within a reasonable time before the filing of the registration
statement or prospectus or amendments or supplements thereto with the
Commission, furnish to counsel selected by the holders of Registrable Securities
copies of such documents proposed to be filed;

          (f)  promptly notify each selling holder of Registrable Securities,
such selling holder's counsel and any underwriter of the happening of any event
which makes any statement made in the registration statement or related
prospectus untrue or which requires the making of any changes in such
registration statement or prospectus so that they will not contain any untrue
statement of a material fact or omit to state any material fact required to be
stated therein or necessary to make the statements therein in the light of the
circumstances under which they were made not misleading; and, subject to Section
8, as promptly as practicable thereafter, prepare and file with the Commission
and furnish a supplement or amendment to such prospectus so that, as thereafter
deliverable to the purchasers of such Registrable Securities, such prospectus
will not contain any untrue statement of a material fact or omit to state a
material fact necessary to make the statements therein, in the light of the
circumstances under which they were made, not misleading;

          (g)  use its reasonable efforts to prevent the issuance of any order
suspending the effectiveness of the registration statement, and if one is issued
use its reasonable efforts to obtain the withdrawal of any such order;

          (h)  if requested by the managing underwriter or underwriters (if
any), any selling holder, or such selling holder's counsel, promptly incorporate
in a prospectus supplement or post-effective amendment such information as such
Person requests to be included therein with respect to the securities being sold
by such selling holder to such underwriter or underwriters, the purchase price
being paid therefor by such underwriter or underwriters and with respect to any
other terms of an underwritten offering of the securities to be sold in such
offering, and promptly make all required filings of such prospectus supplement
or post-effective amendment;

          (i)  make available to each selling holder, any underwriter
participating in any disposition pursuant to a registration statement, and any
attorney, accountant or other agent or representative retained by any such
selling holder or underwriter (collectively, the "Inspectors"), all financial
and other records, pertinent corporate documents and properties of the Company,
as shall be reasonably necessary to enable them to exercise their due diligence
responsibility, and cause the Company's officers, directors and employees to
supply all information reasonably requested by any such Inspector in connection
with such registration statement;

          (j)  enter into any reasonable underwriting agreement required by the
proposed underwriter(s) for the selling holders, if any;

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          (k)  cause the securities covered by such registration statement to be
listed on the securities exchange or quoted on the quotation system on which the
Common Stock of the Company is then listed or quoted; and

          (l)  during the period when the prospectus is required to be delivered
under the Securities Act, file all documents required to be filed with the
Commission pursuant to Sections 13(a), 13(c), 14, or 15(d) of the Exchange Act.

     5.   EXPENSES.
          --------

     All expenses incurred by the Company or the holders of Registrable
Securities in effecting the registrations provided for in Sections 2 and 3,
including, without limitation, all registration and filing fees, printing
expenses, fees and disbursements of counsel for the Company, and one counsel for
the holders participating in such registration as a group (selected by a
majority in interest of the holders of Registrable Securities who participate in
the registration), underwriting expenses (other than fees, commissions or
discounts), expenses of any audits incident to or required by any such
registration and expenses of complying with the securities or blue sky laws of
any jurisdictions (all of such expenses referred to as "Registration Expenses"),
shall be paid by the Company.

     6.   INDEMNIFICATION.
          ---------------

          (a)  The Company shall indemnify and hold harmless each selling holder
of Registrable Securities, each underwriter (as defined in the Securities Act),
and directors, officers, employees and agents of any of them, and each other
Person who participates in the offering of such securities and each other
Person, if any, who controls (within the meaning of the Securities Act) such
seller, underwriter or participating Person (individually and collectively, the
"Indemnified Person") against any losses, claims, damages, expenses or
liabilities (collectively, the "liability"), joint or several, to which such
Indemnified Person may become subject under the Securities Act or any other
statute or at common law, insofar as such liability (or action in respect
thereof) arises out of or is based upon (i) any untrue statement or alleged
untrue statement of any material fact contained, on the effective date thereof,
in any registration statement under which such securities were registered under
the Securities Act, any preliminary prospectus or final prospectus contained
therein, or any amendment or supplement thereto, or (ii) any omission or alleged
omission to state therein a material fact required to be stated therein or
necessary to make the statements therein not misleading, or (iii) any violation
by the Company of the Securities Act, any state securities or "blue sky" laws or
any sale or regulation thereunder in connection with such registration. Except
as otherwise provided in Section 6(d), the Company shall reimburse each such
Indemnified Person in connection with investigating or defending any such
liability; provided, however, that the Company shall not be liable to any
Indemnified Person in any such case to the extent that any such liability arises
out of or is based upon any untrue statement or alleged untrue statement or
omission or alleged omission made in such registration statement, preliminary or
final prospectus, or amendment or supplement thereto in reliance upon and in
conformity with information furnished in writing to the Company by such Person
specifically for use therein; and provided further, that the Company shall not
be required to indemnify any Person against any liability arising from any
untrue or misleading statement or omission contained in any preliminary
prospectus if such deficiency is corrected in the final

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prospectus or for any liability which arises out of the failure of any Person to
deliver a prospectus as required by the Securities Act regardless of any
investigation made by or on behalf of such Indemnified Person and shall survive
transfer of such securities by such seller.

          (b)  Each holder of any securities included in such registration being
effected shall indemnify and hold harmless each other selling holder of any
securities, the Company, its directors and officers, each underwriter and each
other Person, if any, who controls (within the meaning of the Securities Act)
the Company or such underwriter (individually and collectively also the
"Indemnified Person"), against any liability, joint or several, to which any
such Indemnified Person may become subject under the Securities Act or any other
statute or at common law, insofar as such liability (or actions in respect
thereof) arises out of or is based upon (i) any untrue statement or alleged
untrue statement of any material fact contained, on the effective date thereof,
in any registration statement under which securities were registered under the
Securities Act at the request of such selling holder, any preliminary prospectus
or final prospectus contained therein, or any amendment or supplement thereto,
or (ii) any omission or alleged omission by such selling holder to state therein
a material fact required to be stated therein or necessary to make the
statements therein not misleading, in the case of (i) and (ii) to the extent,
but only to the extent, that such untrue statement or alleged untrue statement
or omission or alleged omission was made in such registration statement,
preliminary or final prospectus, amendment or supplement thereto in reliance
upon and in conformity with information furnished in writing to the Company by
such selling holder specifically for use therein. Such selling holder shall
reimburse any Indemnified Person for any legal fees incurred in investigating or
defending any such liability; provided, however, that in no event shall the
liability of any holder for indemnification under this Section 6 in its capacity
as a seller of Registrable Securities exceed the lesser of (i) that proportion
of the total of such losses, claims, damages, expenses or liabilities
indemnified against equal to the proportion of the total securities sold under
such registration statement which are being sold by such holder, or (ii) an
amount equal to the proceeds to such holder of the securities sold in any such
registration; and provided further, however, that no selling holder shall be
required to indemnify any Person against any liability arising from any untrue
or misleading statement or omission contained in any preliminary prospectus if
such deficiency is corrected in the final prospectus or for any liability which
arises out of the failure of any Person to deliver a prospectus as required by
the Securities Act.

          (c)  In the event the Company, any selling holder or other Person
receives a complaint, claim or other notice of any liability or action, giving
rise to a claim for indemnification under Sections 6(a) or (b) above, the Person
claiming indemnification under such paragraphs shall promptly notify the Person
against whom indemnification is sought of such complaint, notice, claim or
action, and such indemnifying Person shall have the right to investigate and
defend any such loss, claim, damage, liability or action.

          (d)  If the indemnification provided for in this Section 6 for any
reason is held by a court of competent jurisdiction to be unavailable to an
Indemnified Person in respect of any liability referred to therein, then each
indemnifying party under this Section 6, in lieu of indemnifying such
indemnified party thereunder, shall contribute to the amount paid or payable by
such indemnified party as a result of such liability (i) in such proportion as
is appropriate to reflect the relative benefits received by the Company, the
selling holders and the underwriters

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from the offering of Registrable Securities or (ii) if the allocation provided
by clause (i) above is not permitted by applicable law, in such proportion as is
appropriate to reflect not only the relative benefits referred to in clause (i)
above but also the relative fault of the Company, the selling holders and the
underwriters in connection with the statements or omissions which resulted in
such liability, as well as any other relevant equitable considerations. The
relative benefits received by the Company, the selling holders and the
underwriters shall be deemed to be in the same respective proportions that the
net proceeds from the offering (before deducting expenses) received by the
Company and the selling holders and the underwriting discount received by the
underwriters, in each case as set forth in the table on the cover page of the
applicable prospectus, bear to the aggregate public offering price of the
securities. The relative fault of the Company, the selling holders and the
underwriters shall be determined by reference to, among other things, whether
the untrue or alleged untrue statement of a material fact or the omission or
alleged omission to state a material fact relates to information supplied by the
Company, the selling holders, or the underwriters and the parties' relative
intent, knowledge, access to information and opportunity to correct or prevent
such statement or omission.

     The Company, each selling holder and the underwriters agree that it would
not be just and equitable if contribution to this Section 6 were determined by
pro rata or per capita allocation or by any other method of allocation which
does not take account the equitable considerations referred to in the
immediately preceding paragraph. In no event, however, shall a selling holder be
required to contribute under this Section 6(d) in excess of the lesser of (i)
that proportion of the total of such liability indemnified against equal to the
proportion of the total Registrable Securities sold under such registration
statement which are being sold by such holder or (ii) the net proceeds received
by such holder from its sale of Registrable Securities under such registration
statement. No Person found guilty of fraudulent representation (within the
meaning of Section 11(f) of the Securities Act) shall be entitled to
contribution from any person who was not found guilty of such fraudulent
misrepresentation.

          (e)  The amount paid by an indemnifying party or payable to an
Indemnified Person as a result of the losses, claims, damages, expenses and
liabilities referred to in this Section 6 shall be deemed to include, subject to
limitations set forth above, any legal or other expenses reasonably incurred by
such indemnified party in connection with investigating or defending any such
action or claim, payable as the same are incurred. The indemnification and
contribution provided for in this Section 6 will remain in full force and effect
regardless of any investigation made by or on behalf of the indemnified parties
or any other officer, director, employee, agent or controlling person of the
indemnified parties. No indemnifying party, in the defense of any such claim or
litigation, shall enter into a consent or entry of any judgment or enter into a
settlement without the consent of the Indemnified Person, which consent will not
be unreasonably withheld or delayed.

     7.   AMENDMENTS.
          ----------

     The provisions of this Agreement may be amended, and the Company may take
any action herein prohibited or omit to perform any act herein required to be
performed by it, only if the Company has obtained the written consent of the
holders of at least a majority of the Registrable Securities. For the purposes
of this Agreement and all agreements executed pursuant hereto, no course of
dealing between or among any of the parties hereto and no delay on the part

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of any party hereto in exercising any rights hereunder or thereunder shall
operate as a waiver of the rights hereof and thereof.

     8.   SUSPENSION PERIOD.
          -----------------

          (a)  Notwithstanding anything to the contrary set forth in this
Agreement, the Company's obligation under this Agreement to use reasonable
efforts to cause a registration statement to be made or to become effective or
to amend or supplement a registration statement shall be suspended during any
period in which a transaction or occurrence (a "Suspension Event") would require
additional disclosure of material information by the Company in the registration
statement (such period being hereinafter referred to as a "Suspension Period")
that would make it inadvisable to cause the registration statement to be made or
to become effective or to amend or supplement the registration statement, but in
no event will the Suspension Period exceed ninety (90) days. In the event any
holder of Registrable Securities requests registration during a Suspension
Event, the Company shall notify the Holder of the existence of such Suspension
Event.

          (b)  Following the effectiveness of a Company registration statement,
the holders of Registrable Securities agree that they will not effect any sales
of the Registrable Securities pursuant to the registration statement at any time
after they have received notice from the Company to suspend sales (i) as a
result of and during a Suspension Period or (ii) for a reasonable time so that
the Company may correct or update the registration statement pursuant to Section
4(f). The holders of Registrable Securities may recommence effecting sales of
the Registrable Securities pursuant to the registration statement following
further notice to such effect from the Company.

     9.   MARKET STAND-OFF.
          ----------------

     Each holder of Registrable Securities agrees if requested by the Company
and an underwriter in connection with any public offering of the Company, not to
directly or indirectly offer, sell, contract to sell, sell any option or
contract to purchase, purchase any option or contract to sell, grant any option,
right or warrant for the sale of or otherwise dispose of or transfer any shares
held by it for such period, not to exceed ninety (90) days following the
effective date of the relevant registration statement, as may be specified by
the Company or such underwriter.

     10.  TRANSFERABILITY OF REGISTRATION RIGHTS.
          --------------------------------------

     The registration rights set forth in this Agreement are transferable to
each transferee of Registrable Securities; provided, that each subsequent holder
of Registrable Securities must consent in writing to be bound by the terms and
conditions of this Agreement in order to acquire the rights granted pursuant to
this Agreement.

     11.  GRANT OF ADDITIONAL REGISTRATION RIGHTS.
          ---------------------------------------

     Other than permitted transferees of Registrable Securities under Section
10, the Company shall not, without the prior written consent of holders of at
least a majority of the Registrable Securities, (a) allow purchasers of the
Company's securities to become a party to this Agreement

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or (b) grant any other registration rights other than any incidental or so
called piggyback registration rights to any third parties that are not
inconsistent with the terms of this Agreement.

     12.  MISCELLANEOUS.
          -------------

          (a)  Notices. All notices, requests, demands and other communications
provided for hereunder shall be in writing and mailed (by first class registered
or certified mail, postage prepaid), telegraphed, sent by express overnight
courier service or electronic facsimile transmission (with a copy by mail), or
delivered to the applicable party at the addresses indicated in the Purchase
Agreement or, in the case of a transferee holder, at the address of such holder
as set forth in the books of the Company or at such other address as shall be
designated by such Person in a written notice to other parties complying as to
delivery with the terms of this subsection (a). All such notices, requests,
demands and other communications shall, when mailed, telegraphed or sent,
respectively, be effective (i) two days after being deposited in the mail, (ii)
one day after being delivered to the telegraph company or deposited with the
express overnight courier service or (iii) upon receipt, if sent by electronic
facsimile transmission.

          (b)  Governing Law. This Agreement shall be governed by and construed
in accordance with the laws of the State of Delaware, without giving effect to
conflict of laws principles thereof.

          (c)  Counterparts. This Agreement may be executed in two or more
counterparts, each of which shall be deemed an original, but all of which
together shall constitute one and the same instrument.

          (d)  Severability. If any provision of this Agreement shall be held to
be illegal, invalid or unenforceable, such illegality, invalidity or
unenforceability shall attach only to such provision and shall not in any manner
affect or render illegal, invalid or unenforceable any other provision of this
Agreement, and this Agreement shall be carried out as if any such illegal,
invalid or unenforceable provision were not contained herein.

          (e)  Integration. This Agreement, including the exhibits, documents
and instruments referred to herein or therein, constitutes the entire agreement
among the parties with respect to the subject matter.

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     IN WITNESS WHEREOF, the parties hereto have caused this Second Amended and
Restated Registration Rights Agreement to be duly executed as of the date first
set forth above.

                                      COMPANY:

                                      SEGUE SOFTWARE, INC.

                                      By:
                                          -------------------------------------
                                          Joseph Krivickas
                                          President and Chief Executive Officer

                                      INVESTOR:

                                      S-7 ASSOCIATES, LLC

                                      By:
                                          -------------------------------------
                                          James H. Simons
                                          Managing Member

                                      -----------------------------------------
                                      Name: Dr. Howard L. Morgan

                                      -----------------------------------------
                                      Name: Isaac J. Mayer<PAGE>

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                                                                   EXHIBIT 10.28

                   SERIES C PREFERRED STOCK PURCHASE AGREEMENT

                                 BY AND BETWEEN

                              SEGUE SOFTWARE, INC.

                                       AND

                                  THE INVESTOR

                                AS DEFINED HEREIN

                          DATED AS OF NOVEMBER 24, 2003

--------------------------------------------------------------------------------

<PAGE>

                              Segue Software, Inc.

                   SERIES C PREFERRED STOCK PURCHASE AGREEMENT

                                November 24, 2003

                                TABLE OF CONTENTS

SECTION I - PURCHASE AND SALE OF SHARES....................................... 1
       1.1      PURCHASE AND SALE OF SERIES C PREFERRED STOCK; CLOSING........ 1

SECTION II - REPRESENTATIONS AND WARRANTIES OF THE COMPANY.................... 1
       2.1      ORGANIZATION AND CORPORATE POWER.............................. 1
       2.2      AUTHORIZATION AND NON-CONTRAVENTION........................... 2
       2.3      AUTHORIZED AND OUTSTANDING STOCK.............................. 2
       2.4      NO BROKERS OR FINDERS......................................... 3

SECTION III- REPRESENTATIONS AND WARRANTIES OF THE INVESTOR................... 4
       3.1      AUTHORIZATION................................................. 4
       3.2      PURCHASE ENTIRELY FOR OWN ACCOUNT............................. 4
       3.3      DISCLOSURE OF INFORMATION..................................... 4
       3.4      ACCREDITED INVESTOR........................................... 4
       3.5      RESTRICTED SECURITIES......................................... 4
       3.6      INVESTMENT BANKING; BROKERAGE FEES............................ 4

SECTION IV - CONDITIONS TO CLOSING............................................ 5
       4.1      EFFECTIVENESS OF SERIES C PREFERRED STOCK TERMS............... 5
       4.2      DELIVERY OF DOCUMENTS......................................... 5
       4.3      APPROVALS AND CONSENTS........................................ 5

SECTION V - MISCELLANEOUS..................................................... 5
       5.1      SURVIVAL OF REPRESENTATIONS AND WARRANTIES.................... 5
       5.2      ENTIRE AGREEMENT.............................................. 5
       5.3      AMENDMENTS, WAIVERS AND CONSENTS.............................. 6
       5.4      NOTICES AND DEMANDS........................................... 6
       5.5      SEVERABILITY.................................................. 6
       5.6      COUNTERPARTS.................................................. 7
       5.7      EFFECT OF HEADINGS............................................ 7
       5.8      GOVERNING LAW................................................. 7

EXHIBITS

Exhibit A       Form of Certificate of Designations

Schedule A      Wire Transfer Instructions

                                      (i)

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                   SERIES C PREFERRED STOCK PURCHASE AGREEMENT

       THIS SERIES C PREFERRED STOCK PURCHASE AGREEMENT is made as of November
24, 2003, by and among Segue Software, Inc., a Delaware corporation (the
"Company") and Isaac J. Mayer (the "Investor").

       WHEREAS, the Company has agreed to sell, and the Investor has agreed to
purchase, 333,333 shares of the Company's Series C Preferred Stock, par value
$0.01 per share (the "Series C Preferred Stock"), for the purchase price
specified herein in accordance with the terms and provisions hereof.

       NOW, THEREFORE, for good and valuable consideration, the receipt and
sufficiency of which are hereby acknowledged, the parties hereby agree as
follows:

                     SECTION I - PURCHASE AND SALE OF SHARES
                     ---------------------------------------

       1.1    PURCHASE AND SALE OF SERIES C PREFERRED STOCK; CLOSING. Subject to
the terms and conditions of this Agreement and in reliance on the
representations, warranties and covenants herein set forth, the Company agrees
to issue and sell to the Investor, and the Investor agrees to purchase from the
Company, an aggregate of 333,333 shares of Series C Preferred Stock for an
aggregate purchase price equal to $1,000,000 (the "Purchase Price"). The Series
C Preferred Stock shall have the rights, preferences and other terms set forth
in the certificate of designations of the Company (the "Certificate of
Designations") attached as Exhibit A hereto. The shares of Series C Preferred
Stock purchased pursuant to this Section 1.1 are hereinafter referred to as the
"Shares." Subject to the satisfaction or waiver of the conditions set forth
herein, the purchase of the Shares shall be made at a closing (the "Closing") to
be held on the date hereof. At the Closing, the Company will deliver to the
Investor one or more certificates representing the Shares purchased by the
Investor against payment of the Purchase Price to the Company by wire transfer
payable in immediately available funds as set forth on Schedule A.

           SECTION II - REPRESENTATIONS AND WARRANTIES OF THE COMPANY
           ----------------------------------------------------------

       In order to induce the Investor to enter into this Agreement and
consummate the transactions contemplated hereby, the Company hereby makes to the
Investor the following representations and warranties.

       2.1    ORGANIZATION AND CORPORATE POWER. The Company is a corporation
duly organized, validly existing and in good standing under the laws of the
State of Delaware. The Company has all requisite corporate power and authority
to own its properties, to carry on its business as presently conducted, to enter
into and perform this Agreement and the agreements, documents and instruments
contemplated hereby (together, the "Transaction Documents") to which it is a
party and to carry out the transactions contemplated hereby and thereby. The
Company is duly licensed or qualified to do business as a foreign corporation in
each jurisdiction wherein the character of its property, or the nature of the
activities presently conducted by it, makes such qualification necessary, except
where the failure to be so licensed or qualified would

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not have a material adverse effect on the financial condition, business or
results of operations of the Company (a "Material Adverse Effect"). The Company
is not in violation of any term or provision of its certificate of
incorporation, as restated (the "Certificate of Incorporation"), or its by-laws
(the "By-laws"), each as in effect as of this date.

       2.2    AUTHORIZATION AND NON-CONTRAVENTION. The Transaction Documents are
valid and binding obligations of the Company, enforceable in accordance with
their terms, except as enforceability may be limited by applicable bankruptcy,
insolvency, moratorium, reorganization or similar laws, from time to time in
effect, which affect enforcement of creditors' rights generally. The execution,
delivery and performance of the Transaction Documents, the sale and delivery of
the Shares in accordance with this Agreement and, if the Shares are converted in
accordance with the Certificate of Incorporation (as amended to date, including
by any Certificates of Designations, the "Certificate of Incorporation"), the
issuance of the common stock, par value $0.01 per share (the "Common Stock")
received upon conversion of the Series C Preferred Stock (the "Conversion
Shares"), have been duly authorized by all necessary corporate or other action
of the Company and its stockholders. The execution, delivery and performance of
the Transaction Documents, including, without limitation, the sale and delivery
of the Shares in accordance with this Agreement and, if the Shares are
converted, the issuance of the Conversion Shares in accordance with the
Certificate of Incorporation and the performance of any transactions
contemplated by the Transaction Documents will not (i) violate, conflict with or
result in a default (whether after the giving of notice, lapse of time or both)
under any contract or obligation to which the Company is a party or by which it
or its assets are bound, or any provision of the Certificate of Incorporation or
By-Laws, or cause the creation of any lien or encumbrance upon any of the assets
of the Company, except for those which would not have a Material Adverse Effect;
(ii) violate, conflict with or result in a default (whether after the giving of
notice, lapse of time or both) under, any provision of any law, regulation or
rule, or any order of, or any restriction imposed by any court or other
governmental agency applicable to the Company, except for those which would not
have a Material Adverse Effect; (iii) require from the Company any notice to,
declaration or filing with, or consent or approval of any governmental authority
or other third party other than pursuant to federal or state securities or blue
sky laws; or (iv) accelerate any obligation under, or give rise to a right of
termination of, any agreement, permit, license or authorization to which the
Company is a party or by which it is bound, which acceleration or termination
would have a Material Adverse Effect.

       2.3    AUTHORIZED AND OUTSTANDING STOCK. After giving effect to the
transactions contemplated hereby, the authorized capital stock of the Company
will consist of (i) 30,000,000 shares of its Common Stock, of which 9,925,447
shares were issued and outstanding as of November 21, 2003, and (ii) 9,000,000
shares of preferred stock, of which (a) 4,000,000 shares have been designated as
Series A Preferred Stock, par value $0.01 per share, no shares of which are
outstanding, and (b) 1,500,000 shares have been designated as Series B Preferred
Stock, par value $0.01 per share (the "Series B Preferred Stock"), 801,448
shares of which are issued and outstanding as of November 21, 2003, and (c)
1,500,000 shares have been designated as Series C Preferred Stock, 166,667
shares of which are issued and outstanding. Except as disclosed in the Company
SEC reports (as defined below) and in the Transaction Documents, there are no
outstanding subscriptions, options, warrants, phantom rights, commitments,
agreements, arrangements or commitments of any kind for or relating to the
issuance, or sale of, or

                                       2
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outstanding securities convertible into or exchangeable for, any shares of
capital stock of any class or other equity interests of the Company. Except as
set forth in the Company SEC Reports, the Company has no obligation to purchase,
redeem, or otherwise acquire any of its capital stock or any interests therein.
After giving effect to the transactions contemplated hereby, all of the
outstanding shares of capital stock of the Company will have been duly and
validly authorized and issued and will be fully paid and non-assessable and free
and clear of all liens and encumbrances created by or through the Company.
Assuming the accuracy of the Investor's representations in Section III, the
offer, issuance, sale and delivery of the Shares and Conversion Shares are or
will be exempt from the registration requirements of the Securities Act of 1933,
as amended (the "Act") and the qualification or registration provisions of
applicable state securities laws. The Company has duly and validly authorized
and reserved 1,500,000 shares of Common Stock for issuance upon conversion of
the Series B Preferred Stock, and the shares issuable upon conversion of the
Series B Preferred Stock in accordance with the Certificate of Incorporation
will, upon such conversion and issuance, be validly issued, fully paid and
non-assessable and free and clear of all liens and encumbrances created by or
through the Company. The Company has duly and validly authorized and reserved
166,667 shares of Common Stock for issuance upon conversion of the Series C
Preferred Stock, and the Conversion Shares issued in accordance with the
Certificate of Incorporation will, upon such conversion and issuance, be validly
issued, fully paid and non-assessable and free and clear of all liens and
encumbrances created by or through the Company. There are no preemptive rights,
rights of first refusal, put or call rights or obligations or anti-dilution
rights with respect to the issuance, sale or redemption of the Company's capital
stock, other than rights set forth herein or in the Certificate of
Incorporation. Other than the rights set forth in the Transaction Documents,
there are no rights to have the Company's capital stock registered for sale to
the public pursuant to the laws of any jurisdiction, and there are no agreements
of which the Company is aware relating to the voting of the Company's voting
securities or restrictions on the transfer of the Company's capital stock.

       2.4    NO BROKERS OR FINDERS. No person has or will have, as a result of
the transactions contemplated by this Agreement, any right, interest or claim
against or upon the Company for any commission, fee or other compensation as a
finder or broker because of any act or omission by the Company.

       2.5    COMPANY SEC REPORTS, GOVERNMENT REPORTS, FINANCIAL STATEMENTS.
Since January 1, 2001, the Company and each Company Subsidiary has filed each
report or other filing that it was required to file with the Securities and
Exchange Commission (the "SEC") (together with any permitted filings on Form
8-K, the "Company SEC Reports"). As of their respective dates, each of the
Company SEC Reports was true and correct in all material respects and complied
in all material respects with the Securities Exchange Act of 1934 and the
regulations promulgated thereunder, and did not contain any untrue statement of
a material fact or omit to state any material fact necessary in order to make
the statements made therein, in light of the circumstances under which they were
made, not misleading (except to the extent revised or superseded by a
later-filed Company SEC Report). The financial statements of the Company
included in the Company SEC Reports comply as to form in all material respects
with applicable accounting requirements and the published rules and regulations
of the SEC with respect thereto, have been prepared in accordance with generally
accepted accounting principles ("GAAP") (except, in the case of unaudited
statements, as permitted by Form 10-Q of the SEC) applied on a

                                       3
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consistent basis during the periods involved (except as may be indicated in the
notes thereto) and fairly present the financial position of the Company and its
consolidated subsidiaries as of the dates thereof and their consolidated results
of operations and cash flows for the periods then ended (subject, in the case of
unaudited statements, to normal year-end audit adjustments).

           SECTION III- REPRESENTATIONS AND WARRANTIES OF THE INVESTOR
           -----------------------------------------------------------

       The Investor hereby represents and warrants to the Company as follows:

       3.1    AUTHORIZATION. The Investor has full power and authority to enter
into this Agreement and the Second Amended and Restated Registration Rights
Agreement referred to in Section 4.2(b), and each such agreement constitutes its
valid and legally binding obligation, enforceable in accordance with its terms.

       3.2    PURCHASE ENTIRELY FOR OWN ACCOUNT. This Agreement is made with the
Investor in reliance upon its representation to the Company, which by the
Investor's execution of this Agreement the Investor hereby confirms, that the
Series C Preferred Stock to be received by the Investor and the Conversion
Shares (collectively, the "Securities") will be acquired for investment for the
Investor's own account, not as a nominee or agent, and not with a view to the
resale or distribution of any part thereof in violation of any applicable law,
and that the Investor has no present intention of selling, granting any
participation in or otherwise distributing the same to any other person. By
executing this Agreement, the Investor further represents that it does not have
any contract, undertaking, agreement or arrangement with any person to sell,
transfer or grant participation to such person or to any third person, with
respect to any of the Securities.

       3.3    DISCLOSURE OF INFORMATION. The Investor represents that it has had
an opportunity to ask questions and receive answers from the Company regarding
the terms and conditions of the offering of the Series C Preferred Stock and the
business, properties, prospects and financial condition of the Company.

       3.4    ACCREDITED INVESTOR. The Investor is an "accredited investor"
within the meaning of SEC Rule 501 of Regulation D, as presently in effect.

       3.5    RESTRICTED SECURITIES. The Investor understands that the
Securities are characterized as "restricted securities" under the federal
securities laws inasmuch as they are being acquired from the Company in a
transaction not involving a public offering and that under such laws and
applicable regulations such Securities may be resold without registration under
the Act only in certain limited circumstances. In the absence of any effective
registration statement covering the Securities or an available exemption from
registration under the Act, the Series C Preferred Stock (and any Common Stock
issued on conversion thereof) must be held indefinitely. In this connection, the
Investor represents that it is familiar with Rule 144, as presently in effect,
and understands the resale limitations imposed thereby and by the Act.

       3.6    INVESTMENT BANKING; BROKERAGE FEES. The Investor has not incurred
or taken any action that would cause the Company to be or become liable for any
investment banking

                                       4
<PAGE>

fees, brokerage commissions, broker's or finder's fees or similar compensation
(exclusive of professional fees to lawyers and accountants) in connection with
the transactions contemplated by this Agreement.

                       SECTION IV - CONDITIONS TO CLOSING
                       ----------------------------------

       The obligation of the Investor to purchase and pay for his or its Shares
shall be subject to the fulfillment to the Investor's reasonable satisfaction or
waiver on or before the Closing of the following conditions:

       4.1    EFFECTIVENESS OF SERIES C PREFERRED STOCK TERMS. The terms of the
Series C Preferred Stock as set forth in Exhibit A hereto shall have become by
the filing of the Certificate of Designations with the Secretary of State of the
State of Delaware and shall remain effective as of the Closing.

       4.2    DELIVERY OF DOCUMENTS. The Company shall have executed and/or
delivered to the Investor (or shall have caused to be executed and delivered to
the Investor by the appropriate persons) the following:

              (a)    certificates representing the Shares; and

              (b)    a Second Amended and Restated Registration Rights Agreement
by and among the Company, the Investor, S-7 Associates, LLC ("S-7") and Dr.
Howard L. Morgan ("Morgan" and such agreement, the "Second Amended and Restated
Registration Rights Agreement"), amending and restating that certain Amended and
Restated Registration Rights Agreement, dated as of October 21, 2003, by and
between the Company, S-7 and Morgan.

       4.3    APPROVALS AND CONSENTS. The Company shall have made all filings
with and notifications of governmental authorities, regulatory agencies and
other entities required to be made by it in connection with the execution and
delivery of this Agreement and the performance by it of the transactions
contemplated hereby.

                            SECTION V - MISCELLANEOUS
                            -------------------------

       5.1    SURVIVAL OF REPRESENTATIONS AND WARRANTIES. The representations,
warranties, covenants and agreements made herein or in any certificates or
documents executed in connection herewith shall survive the execution and
delivery hereof and the Closing contemplated hereby and shall bind the
successors and assigns of the relevant party, whether so expressed or not, and
all such covenants, agreements, representations and warranties shall inure to
the benefit of the successors and assigns of the parties hereto and to
transferees of the Shares or Conversion Shares, whether so expressed or not.

       5.2    ENTIRE AGREEMENT. The Transaction Documents constitute the full
and entire understanding and agreement among the parties hereto with respect to
the subject matters hereof and thereof, and any and all other written or oral
agreements existing prior to or contemporaneously herewith are expressly
superseded and canceled.

                                       5
<PAGE>

       5.3    AMENDMENTS, WAIVERS AND CONSENTS. For the purposes of this
Agreement and all agreements, documents and instruments executed pursuant
hereto, except as otherwise specifically set forth herein or therein, no course
of dealing between the Company on the one hand and the Investor on the other and
no delay on the part of any party hereto in exercising any rights hereunder or
thereunder shall operate as a waiver of the rights hereof and thereof. Any term
or provision hereof may be amended, terminated or waived (either generally or in
a particular instance and either retroactively or prospectively) with the
written consent of the Company and the Investor. Any amendment or waiver
effected in accordance with this Section 5.3 shall be binding upon each holder
of Shares purchased under this Agreement at the time outstanding (including
Conversion Shares into which the Shares have been converted), each future holder
of all such securities and the Company.

       5.4    NOTICES AND DEMANDS. All notices, requests, demands and other
communications hereunder shall be in writing and shall be deemed to have been
duly given if faxed (with transmission acknowledgment received), delivered
personally or mailed by certified or registered mail (return receipt requested)
as follows:

      To the Company:      201 Spring Street
                           Lexington, MA  02421
                           Facsimile: 781-402-1099
                           Attention: Samuel J. Gallo,
                                      Senior Vice President and General Counsel

      With a copy to:      Goodwin Procter LLP
                           Exchange Place
                           Boston, MA  02109
                           Attn:  Jeffrey C. Hadden, P.C.
                           Facsimile Number (617) 523-1231

      To the Investor:     ________________________________

                           ________________________________

                           ________________________________

                           ________________________________

                           Facsimile:  ____________________

      With a copy to:      ________________________________

                           Facsimile: _____________________

or to such other address or fax number of which any party may notify the other
parties as provided above. Notices shall be effective as of the date of such
delivery, mailing or fax.

       5.5    SEVERABILITY. Whenever possible, each provision of this Agreement
shall be interpreted in such a manner as to be effective and valid under
applicable law, but if any

                                       6
<PAGE>

provision of this Agreement shall be deemed prohibited or invalid under such
applicable law, such provision shall be ineffective to the extent of such
prohibition or invalidity, and such prohibition or invalidity shall not
invalidate the remainder of such provision or the other provisions of this
Agreement.

       5.6    COUNTERPARTS. This Agreement and any Exhibit or Schedule hereto
may be executed in multiple counterparts, each of which shall constitute an
original but all of which shall constitute but one and the same instrument. One
or more counterparts of this Agreement or any Exhibit or Schedule hereto may be
delivered via telecopier, with the intention that they shall have the same
effect as an original counterpart hereof.

       5.7    EFFECT OF HEADINGS. The descriptive headings in this Agreement
have been inserted for convenience only and shall not be deemed to limit or
otherwise affect the construction of any provision hereof.

       5.8    GOVERNING LAW. This Agreement shall be deemed a contract made
under the laws of the State of Delaware and all disputes, claims or
controversies arising out of this Agreement or the negotiation, validity or
performance hereof or the transactions contemplated herein, shall be construed
under and governed by the laws of such state, without giving effect to its
conflicts of laws principles.

                                       7
<PAGE>

       IN WITNESS WHEREOF, the undersigned have executed this Series C Preferred
Stock Purchase Agreement as of the day and year first above written.

                               COMPANY:

                               SEGUE SOFTWARE, INC.

                               By:
                                   --------------------------------------------
                                   Name:  Joseph Krivickas
                                   Title: President and Chief Executive Officer

                               INVESTOR:

                                   --------------------------------------------
                                   Name: Isaac J. Mayer

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