Document:

EX-4.2

 Exhibit 4.2 

Execution Version 

THERMO FISHER SCIENTIFIC INC., 
 as
Issuer 
 AND 
 THE BANK OF NEW
YORK MELLON TRUST COMPANY, N.A., 
 as Trustee 

TWENTY-FIRST SUPPLEMENTAL INDENTURE 

Dated as of April 2, 2020 

1.750% Senior Notes due 2027 

2.375% Senior Notes due 2032 

 THIS TWENTY-FIRST SUPPLEMENTAL INDENTURE (this “Supplemental Indenture”) is
dated as of April 2, 2020 between THERMO FISHER SCIENTIFIC INC., a Delaware corporation (the “Company”), and THE BANK OF NEW YORK MELLON TRUST COMPANY, N.A., a national banking association, as trustee (the
“Trustee”). 
 RECITALS 

WHEREAS, the Company and the Trustee executed and delivered an Indenture, dated as of November 20, 2009 (the “Base
Indenture” and, as supplemented by this Supplemental Indenture, the “Indenture”), to provide for the issuance by the Company from time to time of unsubordinated debt securities evidencing its unsecured indebtedness. 

WHEREAS, the Company has authorized the issuance of €600,000,000 aggregate principal amount of the Company’s 1.750% Senior Notes due
2027 (the “2027 Notes”) and €600,000,000 aggregate principal amount of the Company’s 2.375% Senior Notes due 2032 (the “2032 Notes” and, together with the 2027 Notes, the “Notes”). 

WHEREAS, the entry into this Supplemental Indenture by the parties hereto is in all respects authorized by the provisions of the Base
Indenture. 
 WHEREAS, the Company desires to enter into this Supplemental Indenture pursuant to Section 9.01 of the Base Indenture to
establish the terms of the Notes in accordance with Section 2.01 of the Base Indenture and to establish the form of the Notes in accordance with Sections 2.01(a)(10) and 2.02 of the Base Indenture. 

WHEREAS, all things necessary to make this Supplemental Indenture a valid and legally binding agreement according to its terms have been done.

 NOW, THEREFORE, for and in consideration of the foregoing premises, the Company and the Trustee, mutually covenant and agree for the
equal and proportionate benefit of the respective Holders from time to time of the Notes as follows: 
 ARTICLE I 

Section 1.1    Defined Terms. 

(1)    Capitalized terms used but not defined in this Supplemental Indenture shall have the meanings ascribed thereto in
the Base Indenture. 
 (2)    A term defined anywhere in this Supplemental Indenture has the same meaning throughout.

 (3)    The singular includes the plural and vice versa. 

  
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 (4)    Headings are for convenience of reference only and do not affect
the interpretation. 
 (5)    As used herein, the following defined terms shall have the following meanings with respect
to the Notes and this Supplemental Indenture only: 
 “Additional Amounts” has the meaning set forth in
Section 1.4(2). 
 “Below Investment Grade Rating Event” means, with respect to a series of Notes, such Notes are
downgraded below an Investment Grade Rating by any two of the Rating Agencies on any date during the period (the “Trigger Period”) commencing 60 days prior to the first public announcement by the Company of the occurrence of a
Change of Control (or pending Change of Control) and ending 60 days following consummation of such Change of Control (which Trigger Period shall be extended so long as the rating of such Notes is under publicly announced consideration for possible
downgrade by at least two of such Rating Agencies on such 60th day, such extension to last with respect to each such Rating Agency until the date on which such Rating Agency considering such possible downgrade either (x) rates such Notes below
an Investment Grade Rating or (y) publicly announces that it is no longer considering such Notes for possible downgrade, provided that no such extension will occur if on such 60th day such Notes have an Investment Grade Rating from at
least two of such Rating Agencies in question and are not subject to review for possible downgrade by such Rating Agencies). 

“Business Day” means any day, other than a Saturday or Sunday, (1) which is not a day on which banking institutions in
The City of New York or London are authorized or required by law, regulation or executive order to close and (2) on which the Trans-European Automated Real-Time Gross Settlement Express Transfer system (the TARGET2 system), or any successor
thereto, is open. 
 “Change of Control” means the occurrence of any of the following: (1) the direct or indirect
sale, transfer, conveyance or other disposition (other than by way of merger or consolidation), in one or a series of related transactions, of all or substantially all of the properties or assets of the Company and its subsidiaries taken as a whole
to any “person” (as that term is used in Section 13(d)(3) of the Exchange Act) other than the Company or one of its direct or indirect wholly-owned subsidiaries; (2) the consummation of any transaction (including, without
limitation, any merger or consolidation) as a result of which any “person” (as that term is used in Section 13(d)(3) of the Exchange Act) becomes the “beneficial owner” (as defined in Rules
13d-3 and 13d-5 under the Exchange Act), directly or indirectly, of more than 50% of the Company’s outstanding Voting Stock or other Voting Stock into which the
Company’s Voting Stock is reclassified, consolidated, exchanged or changed, measured by voting power rather than number of shares; (3) the Company consolidates with, or merges with or into, any “person” or “group” (as
that term is used in Section 13(d)(3) of the Exchange Act), or any “person” or “group” consolidates with, or merges with or into, the Company, in any such event pursuant to a transaction in which any of the Company’s
Voting Stock or the Voting Stock of such other person is converted into or exchanged for cash, securities or other property, other than any such 

  
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transaction where the shares of the Company’s Voting Stock outstanding immediately prior to such transaction constitute, or are converted into or exchanged for, a majority of the Voting
Stock of the surviving person or any direct or indirect parent company of the surviving person immediately after giving effect to such transaction; or (4) the adoption of a plan relating to the Company’s liquidation or dissolution.
Notwithstanding the foregoing, a transaction will not be deemed to involve a Change of Control if (a) the Company becomes a direct or indirect wholly-owned subsidiary of a holding company (which shall include a parent company) and (b)(i) the
holders of the Voting Stock of such holding company immediately following that transaction are substantially the same as the holders of the Voting Stock of the Company immediately prior to that transaction or (ii) no “person” (as that
term is used in Section 13(d)(3) of the Exchange Act) (other than a holding company satisfying the requirements of this sentence) becomes the “beneficial owner” (as defined in Rules 13d-3 and 13d-5 under the Exchange Act), directly or indirectly, of more than 50% of the voting power of the Voting Stock of such holding company immediately following such transaction. 

“Change of Control Triggering Event” means, with respect to either series of Notes, the occurrence of both a Change of
Control and a Below Investment Grade Rating Event. 
 “Common Depositary” means The Bank of New York Mellon, London Branch,
as common depositary for the Depositary. 
 “Comparable Bond Rate” means, for any Optional Redemption Date, the rate per
annum equal to the annual equivalent yield to maturity or interpolated yield to maturity (on a day count basis), computed as of the third Business Day immediately preceding that Optional Redemption Date, of the Comparable Government Issue, assuming
a price for the Comparable Government Issue (expressed as a percentage of its principal amount) equal to the Comparable Price for that Optional Redemption Date. 

“Comparable Government Issue” means, with respect to either series of Notes to be redeemed, the euro-denominated security
issued by the German government selected by an Independent Investment Banker as having an actual or interpolated maturity comparable to the remaining term of the applicable series of Notes to be redeemed (assuming that such Notes to be redeemed
matured on their applicable Par Call Date) that would be utilized, at the time of selection and in accordance with customary financial practice, in pricing new issues of corporate debt securities of comparable maturity to the remaining term of the
applicable series of Notes to be redeemed. 
 “Comparable Price” means, with respect to any Optional Redemption Date,
(a) the average of the Reference Dealer Quotations for such Optional Redemption Date, after excluding the highest and lowest of the Reference Dealer Quotations, (b) if the Company obtains fewer than four Reference Dealer Quotations, the
arithmetic average of those quotations or (c) if the Company obtains only one Reference Dealer Quotation, such Reference Dealer Quotation. 

  
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 “Corporate Trust Office” means the principal office of the Trustee at
which, at any particular time, its corporate trust business shall be conducted, which office is located at the date of this Supplemental Indenture at 500 Ross Street, 12th Floor, Pittsburgh, Pennsylvania, Attention: Corporate Trust Administration,
or at any other time at such other address as the Trustee may designate from time to time by notice to the Company, or the principal corporate trust office of any successor Trustee (or such other address as such successor Trustee may designate from
time to time by notice to the Company). 
 “Depositary” means each of Clearstream Banking, S.A. and Euroclear Bank SA/NV as
operator of the Euroclear System. 
 “euro” or “€” means the single currency introduced at the third
stage of the European Economic and Monetary Union pursuant to the Treaty establishing the European Community, as amended. 

“Fitch” means Fitch Ratings, Limited, and any successor to its rating agency business. 

“ICMA” means the International Capital Markets Association. 

“Independent Investment Banker” means any Reference Dealer appointed by the Company as Independent Investment Banker
(initially, J.P. Morgan Securities plc). 
 “Internal Revenue Code” means the U.S. Internal Revenue Code of 1986, as
amended. 
 “Investment Grade Rating” means a rating by Moody’s equal to or higher than Baa3 (or the equivalent under
a successor rating category of Moody’s) or a rating by S&P equal to or higher than BBB- (or the equivalent under any successor rating category of S&P) or a rating by Fitch equal to or higher than BBB- (or the equivalent under any successor rating category of Fitch). 
 “Moody’s”
means Moody’s Investors Service, Inc., and any successor to its rating agency business. 
 “Optional Redemption Date”
when used with respect to any Note to be redeemed at the Company’s option, means the date fixed for such redemption by or pursuant to Section 1.3 of this Supplemental Indenture. 

“Optional Redemption Price” when used with respect to any Note to be redeemed at the Company’s option, means the price
at which it is to be redeemed pursuant to Section 1.3 of this Supplemental Indenture. 
 “Par Call Date” means
February 15, 2027 in the case of the 2027 Notes; and January 15, 2032 in the case of the 2032 Notes. 

  
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 “Paying Agency Agreement” means the Paying Agency Agreement, dated as of
April 2, 2020, between the Company and the Paying Agent. 
 “Paying Agent” means The Bank of New York Mellon, London
Branch, or any successor. 
 “Primary Bond Dealer” means a broker or dealer of, and/or a market maker in, German government
bonds. 
 “Rating Agencies” means (1) Moody’s, S&P and Fitch; and (2) if any of Moody’s, S&P or
Fitch ceases to rate the applicable series of Notes or fails to make a rating of such Notes publicly available for any reason, a “nationally recognized statistical rating organization” within the meaning of Section 3(a)(62) of the
Exchange Act, selected by the Company (as certified by a resolution of its Board of Directors) as a replacement agency for any of Moody’s, S&P or Fitch, or all of them, as the case may be. 

“Reference Dealer” means each of (i) J.P. Morgan Securities plc, Morgan Stanley & Co. International plc,
Merrill Lynch International and Citigroup Global Markets Limited and their respective affiliates or successors and (ii) one other nationally recognized investment banking firm (or its affiliate) that is a Primary Bond Dealer that the Company
selects in connection with the particular redemption, and each of their respective successors, provided that if at any time any of the above is not a Primary Bond Dealer, the Company will substitute that entity with another nationally
recognized investment banking firm that the Company selects that is a Primary Bond Dealer. 
 “Reference Dealer Quotations”
means, with respect to each Reference Dealer and any Optional Redemption Date, the arithmetic average, as determined by the Independent Investment Banker, of the bid and asked prices for the Comparable Government Issue (expressed in each case as a
percentage of its principal amount) quoted in writing to the Independent Investment Banker by such Reference Dealer at 11:00 a.m., London time, on the third Business Day preceding such Optional Redemption Date. 

“Remaining Scheduled Payments” means, with respect to each Note to be redeemed, the remaining scheduled payments of the
principal thereof and interest thereon that would be due after the related Optional Redemption Date but for such redemption (assuming that such Note to be redeemed matured on its applicable Par Call Date); provided, however, that, if such
Optional Redemption Date is not an interest payment date with respect to such Note, the amount of the next succeeding scheduled interest payment thereon will be reduced by the amount of interest accrued thereon to such Optional Redemption Date. 

“S&P” means S&P Global Ratings, a division of S&P Global, Inc., and any successor to its rating agency business.

  
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 “Treasury Regulations” means the U.S. Treasury Regulations promulgated
under the Internal Revenue Code. 
 “United States person” means any individual who is a citizen or resident of the United
States for U.S. federal income tax purposes, a corporation, partnership or other entity created or organized in or under the laws of the United States, any state of the United States or the District of Columbia (other than a partnership that is not
treated as a United States person under any applicable Treasury Regulations), or any estate or trust the income of which is subject to United States federal income taxation regardless of its source. 

“United States” means the United States of America, the states of the United States, and the District of Columbia. 

Section 1.2    Terms of the Notes. 

The following terms relate to the Notes: 

(1)    The 2027 Notes shall constitute a separate series of Notes having the title “1.750% Senior Notes due
2027” and the 2032 Notes shall constitute a separate series of Notes having the title “2.375% Senior Notes due 2032.” 

(2)    The aggregate principal amount of the 2027 Notes (the “Initial 2027 Notes”) and the 2032 Notes
(the “Initial 2032 Notes,” and, together with the Initial 2027 Notes, the “Initial Notes”) that may be initially authenticated and delivered under the Indenture shall be €600,000,000 and €600,000,000,
respectively. The Company may from time to time, without the consent of the Holders of either series of Notes, issue additional 2027 Notes (in any such case, the “Additional 2027 Notes”) and additional 2032 Notes (in any such case,
the “Additional 2032 Notes” and, together with the Additional 2027 Notes, the “Additional Notes”) having the same terms (except for the issue date, offering price and, if applicable, the first interest payment date)
as the Initial 2027 Notes or the Initial 2032 Notes, as the case may be. Any Additional Notes of a series and the Initial Notes of such series shall constitute a single series under the Indenture; provided that if any Additional Notes of a
series are not fungible with the Initial Notes of such series for U.S. federal income tax purposes, such Additional Notes of such series shall not have the same ISIN or Common Code as the Initial Notes of such series. All references to a series of
Notes shall include both the Initial Notes and any Additional Notes of such series, unless the context otherwise requires. The aggregate principal amount of each of the 2027 Notes and the 2032 Notes shall be unlimited. The entire respective
Outstanding principal amount of the 2027 Notes and the 2032 Notes shall be payable on April 15, 2027 and April 15, 2032, respectively. The principal of each Note payable at maturity or upon earlier redemption shall be paid against
presentation and surrender of such Note at the Corporate Trust Office of the Trustee. 
 (3)    The rate at which the
2027 Notes shall bear interest shall be 1.750% per annum and the rate at which the 2032 Notes shall bear interest shall be 2.375% per annum. The date from which interest shall accrue on the Notes shall be the most recent Interest Payment Date to

  
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which interest has been paid or provided for or, if no interest has been paid, from April 2, 2020. The Interest Payment Dates for the 2027 Notes shall be April 15 of each year,
beginning on April 15, 2020 until the principal is paid or made available for payment, and the Interest Payment Dates for the 2032 Notes shall be April 15 of each year, beginning on April 15, 2020 until the principal is paid or made
available for payment; provided that if any Interest Payment Date for either series of Notes falls on a day that is not a Business Day, the required payment shall be made on the next Business Day as if it were made on the date the payment to
Holders was due and no interest shall accrue on the amount so payable for the period from and after that Interest Payment Date. The regular record date shall be the 15th calendar day, whether or
not a Business Day, immediately preceding the related Interest Payment Date. Interest on the Notes shall be computed by the Company on the basis of an ACTUAL/ACTUAL (ICMA) (as defined in the rulebook of ICMA) day count convention. 

(4)    The Notes shall be issuable in whole in the form of one or more registered Global Securities, without coupons,
which shall be deposited with, or on behalf of, the applicable Depositary and shall be registered in the name of the Common Depositary for, and in respect of interests held through, the applicable Depositary. Each Note shall be substantially in the
form attached hereto as Exhibit A, the terms of which are herein incorporated by reference. The Notes shall be issuable in denominations of €100,000 or any integral multiple of €1,000 in excess thereof. 

(5)    The Notes may be redeemed at the option of the Company prior to the maturity date, as provided in Section 1.3
hereof. 
 (6)    The Notes shall not have the benefit of any sinking fund. 

(7)    Except as provided herein, the Holders shall have no special rights in addition to those provided in the Base
Indenture upon the occurrence of any particular events. 
 (8)    The Notes shall be general unsecured and
unsubordinated obligations of the Company and shall be ranked equally among themselves. 
 (9)    The Notes are not
convertible into shares of common stock or other securities of the Company. 
 (10)    The covenants set forth in
Section 1.4 hereof shall be applicable to the Notes. 
 (11)    The transfer and exchange provisions set forth in
Section 2.05 of the Base Indenture shall be applicable to the Notes. 
 (12)    All payments of principal of, and
interest (including Additional Amounts, if any) and premium (if any) on, the Notes shall be payable in euro; provided, however, that if, on or after March 30, 2020, euro is unavailable to the Company due to the imposition of exchange
controls or other circumstances beyond the Company’s control or if the euro is no longer being 

  
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used by the then member states of the European Economic and Monetary Union that have adopted the euro as their currency or for the settlement of transactions by public institutions of or within
the international banking community, then all payments in respect of the Notes shall be made in U.S. dollars until the euro is again available to the Company or so used. In such circumstances, the amount payable on any date in euro shall be
converted into U.S. dollars at the rate mandated by the U.S. Federal Reserve Board as of the close of business on the second Business Day prior to the relevant payment date or, in the event the U.S. Federal Reserve Board has not mandated a rate of
conversion, on the basis of the most recent U.S. dollar/euro exchange rate published in The Wall Street Journal on or prior to the second Business Day prior to the relevant payment date. Any payment in respect of the Notes so made in U.S. dollars
shall not constitute an Event of Default under the Notes or the Indenture. Neither the Trustee nor the Paying Agent shall have any responsibility for any calculation or conversion in connection with the foregoing. Any references elsewhere in the
Indenture or the Notes to payments being made in euro notwithstanding, payments shall be made in U.S. dollars to the extent set forth in this Section 1.2(12). 

(13)    The Bank of New York Mellon, London Branch, shall initially act as the Paying Agent in accordance with the terms
of the Paying Agency Agreement. The Company hereby initially designates the Corporate Trust Office of the Trustee as the office to be maintained by it where Notes may be presented for payment, registration of transfer or exchange, and where notices
to or demands upon the Company in respect of the Notes or the Indenture may be served. The Security Registrar for the Notes shall initially be the Trustee. Upon notice to the Trustee, the Company may at any time vary or terminate the appointment of
any Paying Agent or Security Registrar, to appoint additional or other Paying Agents or Security Registrars and to approve any change in the office through which any Paying Agent or Security Registrar acts. 

(14)    In order to provide for all payments due on the Notes as the same shall become due, the Company shall cause to be
paid to the Paying Agent, no later than 10:00 a.m. London time on the Business Day prior to the payment date of each Note, at such bank as the Paying Agent shall previously have notified the Company, in immediately available funds sufficient to meet
all payments due on such Notes. 
 (15)    Notwithstanding any other provision of this Supplemental Indenture, the
Trustee and Paying Agent shall be entitled to make a deduction or withholding from any payment which it makes under this Supplemental Indenture for or on account of any present or future taxes, duties or charges if and to the extent so required by
any applicable law and any current or future regulations or agreements thereunder or official interpretations thereof or any law implementing an intergovernmental approach thereto or by virtue of the relevant Holder failing to satisfy any
certification or other requirements in respect of the Notes, in which event the Trustee or Paying Agent shall make such payment after such withholding or deduction has been made and shall account to the relevant authorities for the amount so
withheld or deducted and shall have no obligation to gross up any payment hereunder or pay any additional amount as a result of such withholding tax. 

  
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 Section 1.3    Optional Redemption. 

(a)    The provisions of Article III of the Base Indenture, as amended by the provisions of this Supplemental Indenture,
shall apply to the Notes with respect to this Section 1.3. 
 (b)    Prior to their applicable Par Call Date, the
Notes of either series shall be redeemable, in whole at any time or in part from time to time, at the Company’s option. Upon redemption of the Notes of either series, the Company shall pay an Optional Redemption Price equal to the greater of:

  

	 	(i)	 100% of the principal amount of the Notes of such series to be redeemed, and 

 

	 	(ii)	 the sum of the present values of the Remaining Scheduled Payments of the Notes of such series to be redeemed,
discounted to the Optional Redemption Date on an annual basis (ACTUAL/ACTUAL (ICMA)) using a discount rate equal to the Comparable Bond Rate, plus 40 basis points, in the case of the 2027 Notes, and 45 basis points, in the case of the 2032 Notes;

 plus, in each case, in addition to such Optional Redemption Price, accrued and unpaid interest on the Notes being redeemed, if
any, to, but excluding, the Optional Redemption Date. 
 On and after the applicable Par Call Date, the Notes of each series shall be
redeemable, in whole at any time or in part from time to time, at the Company’s option, at an Optional Redemption Price equal to 100% of the principal amount of the Notes to be redeemed, plus accrued and unpaid interest, if any, to, but
excluding, the Optional Redemption Date. 
 (c)    The Notes of either series shall also be redeemable, in whole, but
not in part, at any time at the Company’s option, at an Optional Redemption Price equal to 100% of the principal amount of the Notes, plus, accrued and unpaid interest and any Additional Amounts thereon, if the Company determines that
(A) as a result of any change or amendment to the laws, treaties, regulations or rulings of the United States of America or any political subdivision or taxing authority thereof, which change or amendment is announced and becomes effective on
or after March 30, 2020, there is a material probability that the Company has or will become obligated to pay Additional Amounts of such series or (B) on or after March 30, 2020, any change in the official application, enforcement or
interpretation of those laws, treaties, regulations or rulings, including a holding by a court of competent jurisdiction in the United States or any other action, taken by any taxing authority or a court of competent jurisdiction in the United
States, whether or not such action was taken or made with respect to the Company, results in a material probability that the Company has or will become obligated to pay Additional Amounts on any Notes of such series; provided that the Company
determines, in its business judgment, that the obligation to pay such Additional Amounts cannot be avoided by use of reasonable measures available to the Company, not including substitution of the obligor under

  
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such Notes. Prior to the mailing of any notice of such a redemption, the Company shall deliver to the Trustee (1) an Officer’s Certificate stating that the Company is entitled to effect
such a redemption and setting forth a statement of facts showing that the conditions precedent to the right of the Company to so redeem have occurred and (2) an Opinion of Counsel to such effect based on such statement of facts. 

(d)    Notwithstanding the foregoing, installments of interest on any series of Notes whose Stated Maturity is on or prior
to the Optional Redemption Date shall be payable on the applicable Interest Payment Date to the Holders of such Notes registered as such at the close of business on the applicable regular record date pursuant to the Notes and the Indenture. 

(e)    On and after the applicable Optional Redemption Date for either series of Notes, interest shall cease to accrue on
the Notes to be redeemed or any portion thereof called for redemption, unless the Company defaults in the payment of the Optional Redemption Price and accrued and unpaid interest and Additional Amounts, if any. No later than 10:00 a.m. London time
on the Business Day prior to the Optional Redemption Date for any Notes to be redeemed, the Company shall deposit with the Trustee or Paying Agent, funds sufficient to pay the Optional Redemption Price of such Notes on the Optional Redemption Date,
and (except if the date fixed for redemption shall be an Interest Payment Date) accrued and unpaid interest and Additional Amounts, if any. If less than all of the Notes of either series are to be redeemed, the Notes to be redeemed shall be
selected, in the case of global securities, in accordance with applicable Depositary procedures and, in the case of definitive securities in a manner the trustee deems fair and appropriate, unless otherwise required by law or applicable stock
exchange requirements. 
 (f)    Notice of any optional redemption shall be transmitted at least 15 days but not more
than 60 days before the applicable Optional Redemption Date to each Holder of the Notes to be redeemed; provided, however, that the Company shall notify the Trustee of the Optional Redemption Date at least 15 days prior to the date of the
giving of such notice (unless a shorter notice shall be satisfactory to the Trustee). Any notice may, at the Company’s discretion, be subject to the satisfaction or waiver of one or more conditions precedent. In that case, the notice shall
state the nature of such condition precedent. Such notice shall be provided in accordance with Section 3.02 of the Base Indenture. If the Optional Redemption Price cannot be determined at the time such notice is to be given, the actual Optional
Redemption Price applicable to the Notes that are being redeemed, calculated as described above in clause (b), shall be set forth in an Officers’ Certificate of the Company delivered to the Trustee no later than two (2) Business Days prior
to the Optional Redemption Date. Notice of redemption having been given as provided in the Indenture, the Notes called for redemption shall (subject to the satisfaction or waiver of any applicable condition precedent), on the Optional Redemption
Date, become due and payable at the Optional Redemption Price, plus accrued and unpaid interest and Additional Amounts, if any, to, but excluding, the Optional Redemption Date. 

  
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 Section 1.4    Additional Covenants. 

The following additional covenants shall apply with respect to the Notes so long as any of the Notes remain Outstanding: 

(1)    Change of Control Triggering Event. 

(a)    If a Change of Control Triggering Event occurs with respect to either series of the Notes, unless the Company shall
have redeemed such series of the Notes in full, as set forth in Section 1.3 of this Supplemental Indenture or the Company shall have defeased such series of the Notes or have satisfied and discharged such series of the Notes, as set forth in
Article XI of the Base Indenture, the Company shall make an offer (a “Change of Control Offer”) to each Holder of the applicable series of the Notes to repurchase any and all of such Holder’s Notes of such series at a
repurchase price in cash equal to 101% of the aggregate principal amount of the Notes to be repurchased (such principal amount to be equal to €100,000 or any integral multiple of €1,000 in excess thereof), plus accrued and unpaid interest,
if any, on the Notes to be repurchased up to, but excluding, the date of repurchase (the “Change of Control Payment”). Within 30 days following any Change of Control Triggering Event, notice shall be delivered to the Holders of
Notes of such series describing the transaction or transactions that constitute the Change of Control Triggering Event and offering to repurchase such Notes on the date specified in the notice, which date will be no earlier than 15 days and no later
than 60 days from the date such notice is delivered (the “Change of Control Payment Date”). Notwithstanding the foregoing, installments of interest on any series of Notes whose Stated Maturity is on or prior to the Change of Control
Payment Date shall be payable on the applicable Interest Payment Date to the Holders of such Notes registered as such at the close of business on the applicable regular record date pursuant to the Notes and the Indenture. 

(b)    On the Change of Control Payment Date, the Company shall, to the extent lawful: 

 

	 	(i)	 accept for payment all Notes or portions of Notes of the applicable series properly tendered pursuant to the
Change of Control Offer; 

  

	 	(ii)	 deposit with the Trustee or a paying agent an amount equal to the Change of Control Payment in respect of all
Notes or portions of Notes of the applicable series properly tendered; and 

  

	 	(iii)	 deliver or cause to be delivered to the Trustee the Notes properly accepted, together with an Officer’s
Certificate stating (1) the aggregate principal amount of such series of Notes or portions of such series of Notes being repurchased, (2) that all conditions precedent contained herein to make a Change of Control Offer have been complied
with and (3) that the Change of Control Offer has been made in compliance with the Indenture. 

  
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 The Company shall comply in all material respects with the requirements of Rule 14e-1 under the Exchange Act and any other securities laws and regulations thereunder to the extent such laws and regulations are applicable in connection with the repurchase of the Notes as a result of a Change of
Control Triggering Event. To the extent that the provisions of any such securities laws or regulations conflict with this Section 1.4, the Company shall comply with the applicable securities laws and regulations and shall not be deemed to have
breached its obligations under this Section 1.4 by virtue of any such conflict. 
 (2)    Additional Amounts. 

(a)    All payments of principal and interest in respect of the Notes shall be made free and clear of, and without
deduction or withholding for or on account of any present or future taxes, duties, assessments or other governmental charges of whatsoever nature imposed, levied, collected, withheld or assessed by the United States or any political subdivision or
taxing authority of or in the United States, unless such withholding or deduction is required by law. 
 (b)    The
Company shall pay to a Holder who is not a United States person additional amounts as may be necessary so that every net payment of the principal of and premium, if any, and interest on the Notes to such Holder, after deduction or withholding for or
on account of any present or future tax, assessment or other governmental charge imposed upon such Holder by the United States of America or any taxing authority thereof or therein, shall not be less than the amount provided in the Note to be then
due and payable (such amounts, the “Additional Amounts”); provided, however, that the Company shall not be required to make any payment of Additional Amounts for or on account of: 

 

	 	(i)	 any tax, assessment or other governmental charge that would not have been imposed but for (A) the
existence of any present or former connection (other than a connection arising solely from the ownership of those Notes or the receipt of payments in respect of those Notes) between that Holder (or the beneficial owner for whose benefit such Holder
holds such Notes), or between a fiduciary, settlor, beneficiary of, member or shareholder of, or possessor of a power over, that Holder or beneficial owner, if that Holder or beneficial owner is an estate, trust, partnership or corporation, and the
United States, including that Holder or beneficial owner, or that fiduciary, settlor, beneficiary, member, shareholder or possessor being or having been a citizen or resident or treated as a resident of the United States or being or having been
engaged in trade or business or present in the United States or having had a permanent establishment in the United States or (B) the presentation of a debt security for payment on a date more than 30 days after the later of the date on which
that payment becomes due and payable and the date on which payment is duly provided for; 

  
 13 

	 	(ii)	 any estate, inheritance, gift, sales, transfer, excise, personal property, wealth, capital gains, interest
equalization or similar tax, assessment or other governmental charge; 

  

	 	(iii)	 any tax, assessment or other governmental charge imposed on foreign personal holding company income or by
reason of a Holder (or the beneficial owner for whose benefit such Holder holds such Notes), or a fiduciary, settlor, beneficiary of, member or shareholder of, or possessor of a power over, the Holder or beneficial owner, if that Holder or
beneficial owner is an estate, trust, partnership or corporation, being or having been a passive foreign investment company, a controlled foreign corporation, a foreign tax exempt organization or a personal holding company with respect to the United
States or a corporation that accumulates earnings to avoid U.S. federal income tax; 

  

	 	(iv)	 any tax, assessment or other governmental charge which is payable otherwise than by withholding from payment of
principal of or premium, if any, or interest on the Notes of that Holder; 

  

	 	(v)	 any tax, assessment or other governmental charge required to be withheld by any paying agent from any payment
of principal of or premium, if any, or interest on the Notes of that Holder if such payment can be made without withholding by any other paying agent; 

  

	 	(vi)	 any tax, assessment or other governmental charge which would not have been imposed but for the failure of a
Holder (or the beneficial owner for whose benefit such Holder holds the Notes), or a fiduciary, settlor, beneficiary of, member or shareholder of, or possessor of power over, the holder or beneficial owner, if that Holder or beneficial owner is an
estate, trust, partnership or corporation, or any intermediary through which a beneficial owner holds Notes to comply with certification, information, documentation or other reporting requirements concerning the nationality, residence, identity or
connections with the United States of America of the beneficial owner or any Holder (including, but not limited to, the requirement to provide Internal Revenue Service Forms W-8BEN, Forms W-8BEN-E, Forms W-8ECI, or any subsequent versions thereof or successor thereto, and including, without limitation, any documentation
requirement under an applicable income tax treaty); 

  

	 	(vii)	 any tax, assessment or other governmental charge imposed as a result of a Holder (or the beneficial owner for
whose benefit such Holder holds such Notes), or a fiduciary, settlor, beneficiary of, member or shareholder of, or possessor of a power over, the Holder or beneficial owner, if that Holder or beneficial owner is an estate, trust, partnership or
corporation, being or 

  
 14 

	 	
having been (A) a 10% shareholder (as defined in Section 871(h)(3)(B) of the Internal Revenue Code, and the regulations that may be promulgated thereunder) of the Company or (B) a
controlled foreign corporation that is related to the Company within the meaning of Section 864(d)(4) of the Internal Revenue Code or (C) a bank receiving interest described in Section 881(c)(3)(A) of the Internal Revenue Code;

  

	 	(viii)	 any tax, assessment or other governmental charge that would not have been imposed but for a change in law,
regulation, or administrative or judicial interpretation that becomes effective more than 15 days after the payment becomes due or is duly provided for, whichever occurs later; 

 

	 	(ix)	 any taxes payable under Sections 1471 through 1474 of the Internal Revenue Code (or any amended or successor
version of such Sections), any current or future regulations or other guidance thereunder, or any agreement (including any intergovernmental agreement) entered into in connection therewith; or 

 

	 	(x)	 any combination of items (i), (ii), (iii), (iv), (v), (vi), (vii), (viii) and (ix) above;

 nor shall any Additional Amounts be paid to any Holder that is not the sole beneficial owner of the Notes, or a
portion of the Notes, or that is a fiduciary, partnership or limited liability company to the extent that a beneficial owner with respect to the Holder, a beneficiary or settlor with respect to the fiduciary or a member of that partnership, limited
liability company or a beneficial owner thereof would not have been entitled to the payment of those Additional Amounts had that beneficiary, settlor, member or beneficial owner received directly its beneficial or distributive share of the payment.

 Section 1.5    Events of Default. 

(a)    The provisions of Article VI of the Base Indenture shall be applicable to each series of the Notes, except that
clauses (1) through (7) of Section 6.01(a) shall be modified with respect to Notes of a series as follows: 

(1)    default in the payment of the principal or any premium on such series of Notes when due (whether at
maturity, upon acceleration, redemption or otherwise); 
 (2)    default for 30 days in the payment of
interest on the Notes of such series when due; 
 (3)    (i) failure by the Company to comply with
Section 1.4(1) of this Supplemental Indenture with respect to such series of Notes or (ii) failure by the Company to observe or perform any term of the Indenture applicable to such series of

  
 15 

 
Notes (other than those referred to in (1) or (2) above or (3)(i) above) for a period of 90 days after the Company receives a notice of default stating that the Company is in breach. The
notice required under 3(ii) above must be sent by either the Trustee or Holders of 25% of the principal amount of the applicable series of Notes; 

(4)    (A) failure by the Company to pay indebtedness for money borrowed by the Company or for which the
Company has guaranteed the payment, in an aggregate principal amount of at least $150,000,000, at the later of final maturity and the expiration of any related applicable grace period and such defaulted payment shall not have been made, waived or
extended within 30 days or (B) acceleration of the maturity of any indebtedness for money borrowed by the Company or for which the Company has guaranteed the payment, in an aggregate principal amount of at least $150,000,000, if such
indebtedness has not been discharged in full or such acceleration has not been rescinded or annulled within 30 days; provided, however, that, if the default under the instrument is cured by the Company, or waived by the holders of the
indebtedness, in each case as permitted by the governing instrument, then the Event of Default under the Indenture caused by such default will be deemed likewise to be cured or waived; 

(5)    the entry by a court having competent jurisdiction of: 

(A)    an order for relief in respect of the Company as debtor in an involuntary proceeding under any
applicable Bankruptcy Law and such order shall remain unstayed and in effect for a period of 60 consecutive days; or 

(B)    a final and non-appealable order appointing a Custodian of
the Company, or ordering the winding up or liquidation of the affairs of the Company, and such order shall remain unstayed and in effect for a period of 60 consecutive days; or 

(6)    the commencement by the Company of a voluntary proceeding under any applicable Bankruptcy Law or the
consent by the Company as debtor to the entry of a decree or order for relief in an involuntary proceeding under any applicable Bankruptcy Law, or the filing by the Company as debtor of a consent to an order for relief in any involuntary proceeding
under any Bankruptcy Law, or to the appointment of a Custodian or the making by the Company of an assignment for the benefit of creditors. 

Section 1.6    Execution and Authentications. 

(1)    Section 2.04 of the Base Indenture shall be modified with respect to the Notes as follows: 

The Securities shall be signed on behalf of the Company by any member of the Board of Directors of the Company or by both (a) its
president, chief financial officer or vice president and (b) its secretary, any assistant secretary, its treasurer or any assistant treasurer. Signatures 

  
 16 

 
may be in the form of a manual or facsimile signature. In the case of Definitive Securities of either series, such signatures may be imprinted or otherwise reproduced on such Securities. The
Securities may contain such notations, legends or endorsements required by law, stock exchange rule or usage. Each Security shall be dated the date of its authentication by the Trustee. 

A Security shall not be valid until authenticated manually or by electronic signature by an authorized signatory of the Trustee or by an
Authenticating Agent. Such signature shall be conclusive evidence that the Security so authenticated has been duly authenticated and delivered hereunder and that the holder is entitled to the benefits of this Indenture. At any time and from time to
time after the execution and delivery of this Indenture, the Company may deliver Securities of either series executed by the Company to the Trustee for authentication, together with a written order of the Company for the authentication and delivery
of such Securities, signed by an Officer (an “Authentication Order”), and the Trustee in accordance with such written order shall authenticate and deliver such Securities. 

Notwithstanding the provisions of Section 2.01 and the preceding paragraph, in the case of Securities offered in a Periodic Offering, the
Trustee shall authenticate and deliver such Securities from time to time in accordance with instructions or such other procedures acceptable to the Trustee as may be specified by or pursuant to a supplemental indenture or the written order of the
Company delivered to the Trustee prior to the time of the first authentication of Securities of such series. With respect to Securities of a series subject to a Periodic Offering, the Trustee may conclusively rely, as to the authorization by the
Company of any of such Securities, the forms and terms thereof and the legality, validity, binding effect and enforceability thereof, upon the written order of the Company, Opinion of Counsel, Officer’s Certificate and other documents delivered
pursuant to this Section 2.04 at or prior to the time of the first authentication of Securities of such series unless and until such written order, Opinion of Counsel, Officer’s Certificate or other documents have been superseded or
revoked or expire by their terms. 
 ARTICLE II 

MISCELLANEOUS 

Section 2.1    Business Day. 

If any maturity date or earlier date of redemption for either series of Notes falls on a day that is not a Business Day, the required payment
shall be made on the next Business Day as if it were made on the date the payment to Holders was due and no interest shall accrue on the amount so payable for the period from and after that maturity date or that date of redemption, as the case may
be. 

  
 17 

 Section 2.2    [Reserved]. 

Section 2.3    Confirmation of Indenture. 

The Base Indenture, as supplemented and amended by this Supplemental Indenture, is in all respects ratified and confirmed, and the Base
Indenture, this Supplemental Indenture and all indentures supplemental thereto shall be read, taken and construed as one and the same instrument. 

Section 2.4    Concerning the Trustee. 

In carrying out its responsibilities hereunder, the Trustee shall have all of the rights, protections and immunities which it possesses under
the Indenture. The recitals contained herein and in the Notes, except the Trustee’s certificate of authentication, shall be taken as the statements of the Company, and the Trustee assumes no responsibility for their correctness. The Trustee
makes no representations as to the validity or sufficiency of this Supplemental Indenture or of the Notes. The Trustee shall not be accountable for the use or application by the Company of the Notes or the proceeds thereof. 

Section 2.5    Governing Law. 

This Supplemental Indenture and the Notes shall be deemed to be a contract made under the internal laws of the State of New York, and for all
purposes shall be construed in accordance with the laws of said State. 
 Section 2.6    Separability.

 In case any provision in this Supplemental Indenture shall for any reason be held to be invalid, illegal or unenforceable, the validity,
legality and enforceability of the remaining provisions shall not in any way be affected or impaired thereby. 

Section 2.7    Counterparts. 

This Supplemental Indenture may be executed in any number of counterparts each of which shall be an original, but such counterparts shall
together constitute but one and the same instrument. 
 Section 2.8    No Benefit. 

Nothing in this Supplemental Indenture, express or implied, shall give to any Person other than the parties hereto and their successors or
assigns, and the Holders, any benefit or legal or equitable rights, remedy or claim under this Supplemental Indenture or the Base Indenture. 

  
 18 

 IN WITNESS WHEREOF, the parties hereto have caused this Supplemental Indenture to be duly
executed all as of the day and year first above written. 
  

			
	THERMO FISHER SCIENTIFIC INC.
		
	 By:
	 	/s/ Anthony H. Smith
		 	Name: Anthony H. Smith
		 	Title:Vice President, Tax and Treasury and Treasurer

  
 [Twenty-First
Supplemental Indenture] 

 
			
	THE BANK OF NEW YORK MELLON TRUST COMPANY, N.A., as Trustee
		
	By:	 	/s/ Manjari Purkayastha
		 	Name: Manjari Purkayastha
		 	 Title:Vice President

  
 [Twenty-First
Supplemental Indenture] 

 EXHIBIT A 

[Insert the Global Security legend, if applicable] 

[    ]%1 SENIOR NOTES DUE [    ]2 
  

			
	No. [    ]	  	€[    ]
	ISIN No. [ ]3	  	

 THERMO FISHER SCIENTIFIC INC. 

promises to pay to [        ] or registered assigns, the principal sum of
[        ] Euro on [        ]4. 

Interest Payment Date: [        ]5 

Record Date: [        ]6 

Each holder of this Security (as defined below), by accepting the same, agrees to and shall be bound by the provisions hereof and of the
Indenture described herein, and authorizes and directs the Trustee described herein on such holder’s behalf to be bound by such provisions. Each holder of this Security hereby waives all notice of the acceptance of the provisions contained
herein and in the Indenture and waives reliance by such holder upon said provisions. 
 This Security shall not be entitled to any benefit
under the Indenture, or be valid or become obligatory for any purpose, until the Certificate of Authentication hereon shall have been signed by or on behalf of the Trustee. The provisions of this Security are continued on the reverse side hereof,
and such continued provisions shall for all purposes have the same effect as though fully set forth at this place. 
  

	1 	 2027 Notes: 1.750% and 2032 Notes: 2.375% 

	2 	 2027 Notes: 2027 and 2032 Notes: 2032 

	3 	 2027 Notes: XS2010032022 and 2032 Notes: XS2010032451 

	4 	 2027 Notes: April 15, 2027 and 2032 Notes: April 15, 2032 

	5 	 2027 Notes and 2032 Notes: April 15 

	6 	 2027 Notes and 2032 Notes: March 31 

  
 A-1 

 IN WITNESS WHEREOF, the Company has caused this instrument to be signed in accordance with Section 2.04
of the Base Indenture. 
 Date: [                    ]

  

			
	 THERMO FISHER SCIENTIFIC INC.

		
	 By:
	 	 
		 	 Name:

		 	 Title:

		
	 By:
	 	 
		 	 Name:

		 	 Title:

  
 A-2 

 CERTIFICATE OF AUTHENTICATION 

This is one of the [        ]7 issued by Thermo Fisher Scientific
Inc. of the series designated therein, referred to in the within-mentioned Indenture. 
 Date:
[                    ] 
  

			
	THE BANK OF NEW YORK MELLON TRUST COMPANY, N.A. as Trustee
		
	By:	 	 
		 	 Authorized Signatory

  

	7 	 2027 Notes: 1.750% Senior Notes due 2027 and 2032 Notes: 2.375% Senior Notes due 2032 

  
 A-3 

 Thermo Fisher Scientific Inc. 

[    ]8 

This security is one of a duly authorized series of debt securities of Thermo Fisher Scientific Inc., a Delaware corporation (the “Company”),
issued or to be issued in one or more series under and pursuant to an Indenture for the Company’s unsubordinated debt securities, dated as of November 20, 2009 (the “Base Indenture”), duly executed and delivered by and
among the Company and The Bank of New York Mellon Trust Company, N.A., as trustee (the “Trustee”), as supplemented by the Twenty-First Supplemental Indenture, dated as of April 2, 2020 (the “Supplemental
Indenture”), between the Company and the Trustee. The Notes are subject to a Paying Agency Agreement, dated as of April 2, 2020 (the “Paying Agency Agreement”), between the Company and The Bank of New York Mellon,
London Branch, as paying agent (the “Paying Agent”). The Base Indenture as supplemented and amended by the Supplemental Indenture is referred to herein as the “Indenture.” By the terms of the Base Indenture, the
debt securities issuable thereunder are issuable in series that may vary as to amount, date of maturity, rate of interest and in other respects as provided in the Base Indenture. This security is one of the series designated on the face hereof
(individually, a “Security,” and collectively, the “Securities”), and reference is hereby made to the Indenture for a description of the rights, limitations of rights, obligations, duties and immunities of the
Trustee, the Company and the holders of the Securities (the “Securityholders”). Capitalized terms used herein and not otherwise defined shall have the meanings given them in the Base Indenture or the Supplemental Indenture, as
applicable. 
 1.    Interest. The Company promises to pay interest on the principal amount of this Security at
an annual rate of [ ]%9. The Company will pay interest annually in arrears on [ ]10 of each year (each such day, an “Interest Payment
Date”) until the principal is paid or made available for payment. If any Interest Payment Date, redemption date or maturity date of this Security is not a Business Day, then payment of interest or principal (and premium, if any) shall be
made on the next succeeding Business Day with the same force and effect as if made on the date such payment was due, and no interest shall accrue for the period after such date to the date of such payment on the next succeeding Business Day.
Interest on the Securities will accrue from the most recent date to which interest has been paid or duly made available for payment or, if no interest has been paid, from the date of issuance; provided that, if there is no existing Default in
the payment of interest, and if this Security is authenticated between a regular record date referred to on the face hereof and the next succeeding Interest Payment Date, interest shall accrue from such next succeeding Interest Payment Date; and
provided, further, that the first Interest Payment Date shall be [ ]11. Interest on the Securities shall be computed on the basis of an ACTUAL/ACTUAL (ICMA) (as defined in the rulebook of
ICMA) day count convention. In 
  

	8 	 2027 Notes: 1.750% Senior Notes due 2027 and 2032 Notes: 2.375% Senior Notes due 2032 

	9 	 2027 Notes: 1.750% and 2032 Notes: 2.375% 

	10 	 2027 Notes: April 15 and 2032 Notes: April 15 

	11 	 2027 Notes: April 15, 2020 and 2032 Notes: April 15, 2020

  
 A-4 

 
order to provide for all payments due on the Securities as the same shall become due, the Company shall cause to be paid to the Paying Agent, no later than 10:00 a.m. London time on the Business
Day prior the payment date of each Security, at such bank as the Paying Agent shall previously have notified to the Company, in immediately available funds sufficient to meet all payments due on such Securities. 

2.    Method of Payment. The Company will pay interest on the Securities (except defaulted interest), if any, to
the persons in whose name such Securities are registered at the close of business on the regular record date referred to on the facing page of this Security for such interest installment. In the event that the Securities or a portion thereof are
called for redemption pursuant to an optional redemption or there is a Change of Control Offer, and the Optional Redemption Date or the Change of Control Payment Date, as applicable, is subsequent to a regular record date with respect to any
Interest Payment Date and prior to such Interest Payment Date, interest on such Securities shall instead be paid upon presentation and surrender of such Securities as provided in the Indenture. Subject to Section 1.2 of the Supplemental
Indenture, all payments of principal of, and interest (including Additional Amounts, if any) and premium (if any) on, the Securities shall be payable in euro. 

3.    Paying Agent and Registrar. Initially, The Bank of New York Mellon, London Branch, shall act as the Paying
Agent in accordance with the terms of the Paying Agency Agreement and the Trustee shall act as Security Registrar. Upon prior notice to the Trustee, the Company may change or appoint any Paying Agent or Security Registrar without notice to any
Securityholder. The Company or any of its Subsidiaries may act in any such capacity. 
 4.    Indenture. The
terms of the Securities include those stated in the Indenture and those made part of the Indenture by reference to the Trust Indenture Act of 1939, as amended (“TIA”), as in effect on the date the Indenture is qualified. The
Securities are subject to all such terms, and Securityholders are referred to the Indenture and TIA for a statement of such terms. In the event of a conflict between the terms of the Securities and the terms of the Indenture, the terms of the
Indenture shall prevail. The Securities are unsecured general obligations of the Company and constitute the series designated on the face hereof as the “[ ]12,” initially limited to
€[ ]13 in aggregate principal amount. The Company shall furnish to any Securityholder upon written request and without charge a copy of the Base Indenture and the Supplemental Indenture.
Requests may be made to: Thermo Fisher Scientific Inc., 168 Third Avenue, Waltham, Massachusetts 02451, Attention: Michael A. Boxer. 

5.    Redemption. The Securities may be redeemed at the option of the Company prior to the maturity date, as
provided in Section 1.3 of the Supplemental Indenture. The Company shall not be required to make sinking fund payments with respect to the Securities. 
  

 
  

	12 	 2027 Notes: 1.750% Senior Notes due 2027 and 2032 Notes: 2.375% Senior Notes due 2032 

	13 	 2027 Notes: 600,000,000 and 2032 Notes: 600,000,000 

  
 A-5 

 6.    Payment of Additional Amounts. All payments of principal
and interest in respect of the Securities shall be made free and clear of, and without deduction or withholding for or on account of any present or future taxes, duties, assessments or other governmental charges of whatsoever nature imposed, levied,
collected, withheld or assessed by the United States or any political subdivision or taxing authority of or in the United States, unless such withholding or deduction is required by law. The Company shall pay to a Holder of Securities who is not a
United States person additional amounts as may be necessary so that every net payment of the principal of and premium, if any, and interest on the Securities to such Holder, after deduction or withholding for or on account of any present or future
tax, assessment or other governmental charge imposed upon such Holder by the United States of America or any taxing authority thereof or therein, shall not be less than the amount provided in the Securities to be then due and payable (such amounts,
the “Additional Amounts”); provided, however, that the Company shall not be required to make any payment of Additional Amounts under certain circumstances provided in Section 1.4(2)(b) of the Supplemental Indenture. 

7.    Change of Control Triggering Event. Upon the occurrence of a Change of Control Triggering Event, unless the
Company has redeemed this Security or the Company has defeased this Security or satisfied and discharged this Security, the Holder of this Security shall have the right to require that the Company purchase all or a portion (such principal amount to
be equal to €100,000 or any integral multiple of €1,000 in excess thereof) of this Security at a purchase price equal to 101% of the aggregate principal amount repurchased plus accrued and unpaid interest, if any, on the amount to be
repurchased up to but excluding the date of purchase. Within 30 days following any Change of Control Triggering Event, the Company shall send, by first class mail, a notice to each Holder, in accordance with Section 1.4(1)(a) of the
Supplemental Indenture, with a copy to the Trustee, which notice shall govern the terms of the Change of Control Offer. 

8.    Denominations, Transfer, Exchange. The Securities are in registered form without coupons in the denominations
of €100,000 or any integral multiple of €1,000 in excess thereof. The transfer of Securities may be registered and Securities may be exchanged as provided in the Indenture. The Securities may be presented for exchange or for registration
of transfer (duly endorsed or with the form of transfer endorsed thereon duly executed if so required by the Company or the Security Registrar) at the office of the Paying Agent or at the office of any transfer agent designated by the Company for
such purpose. No service charge shall be made for any registration of transfer or exchange, but a Securityholder may be required to pay any applicable taxes or other governmental charges. If the Securities are to be redeemed, the Company shall not
be required to: (i) issue, register the transfer of, or exchange any Security during a period beginning at the opening of business 15 days before the day of mailing of a notice of redemption of less than all of the outstanding Securities and
ending at the close of business on the day of such mailing; (ii) register the transfer of or exchange any Security or portions thereof selected for redemption, in whole or in part, except the unredeemed portions of any such Security being
redeemed in part; nor (iii) register the transfer of or exchange of a Security between the applicable record date and the next succeeding Interest Payment Date. 

  
 A-6 

 9.    Persons Deemed Owners. The registered Securityholder may be
treated as its owner for all purposes. 
 10.    Repayment to the Company. Any funds or Governmental Obligations
deposited with any paying agent or the Trustee, or then held by the Company, in trust for payment of principal of, premium, if any, or interest on the Securities that are not applied but remain unclaimed by the Holders of such Securities for at
least one year after the date upon which the principal of, premium, if any, or interest on such Securities shall have respectively become due and payable, shall be repaid to the Company, as applicable, or (if then held by the Company) shall be
discharged from such trust. After return to the Company, Holders entitled to the money or securities must look to the Company, as applicable, for payment as unsecured general creditors. 

11.    Amendments, Supplements and Waivers. The Indenture permits, with certain exceptions as therein provided, the
amendment thereof and the modification of the rights and obligations of the Company and the rights of the Holders of the Securities to be affected under the Indenture at any time by the Company and the Trustee with the consent of the Holders of a
majority in principal amount of the Securities at the time Outstanding to be affected. The Indenture also contains provisions permitting the Holders of a majority in principal amount of the Securities at the time Outstanding, on behalf of the
Holders of all Securities, to waive compliance by the Company with certain provisions of the Indenture and certain past defaults under the Indenture and their consequences. Any such consent or waiver by the Holder of this Security shall be
conclusive and binding upon such Holder and upon all future Holders of this Security and of any Security issued upon the registration of transfer hereof or in exchange herefor or in lieu hereof, whether or not notation of such consent or waiver is
made upon this Security. 
 12.    Defaults and Remedies. If an Event of Default with respect to the Securities
occurs and is continuing, the Trustee or the Holders of at least 25% in aggregate principal amount of the Securities then Outstanding, by notice in writing to the Company (and to the Trustee if notice is given by such Holders), may declare the
entire principal of, premium, if any, and accrued interest, if any, of such Securities due and payable immediately. Subject to the terms of the Indenture, if an Event of Default under the Indenture shall occur and be continuing, the Trustee shall be
under no obligation to exercise any of its rights or powers under the Indenture at the request or direction of any of the Holders, unless such Holders have offered the Trustee indemnity satisfactory to it. Upon satisfaction of certain conditions set
forth in the Indenture, the Holders of a majority in principal amount of the Outstanding Securities shall have the right to direct the time, method and place of conducting any proceeding for any remedy available to the Trustee, or exercising any
trust or power conferred on the Trustee, with respect to the Securities. 
 13.    Trustee, Paying Agent and Security
Registrar May Hold Securities. The Trustee, subject to certain limitations imposed by the TIA, or any paying agent or Security Registrar, in 

  
 A-7 

 
its individual or any other capacity, may become the owner or pledgee of Securities with the same rights it would have if it were not Trustee, paying agent or Security Registrar. 

14.    No Recourse Against Others. No recourse under or upon any obligation, covenant or agreement of the
Indenture, or of any Security, or for any claim based thereon or otherwise in respect hereof or thereof, shall be had against any incorporator, stockholder, officer or director, past, present or future as such, of the Company or of any predecessor
or successor corporation, either directly or through the Company or any such predecessor or successor corporation, whether by virtue of any constitution, statute or rule of law, or by the enforcement of any assessment or penalty or otherwise; it
being expressly understood that the Indenture and the obligations issued hereunder and thereunder are solely corporate obligations, and that no such personal liability whatever shall attach to, or is or shall be incurred by, the incorporators,
stockholders, officers or directors as such, of the Company or of any predecessor or successor corporation, or any of them, because of the creation of the indebtedness authorized by the Indenture, or under or by reason of the obligations, covenants
or agreements contained in the Indenture or in the Securities or implied therefrom; and that any and all such personal liability of every name and nature, either at common law or in equity or by constitution or statute, of, and any and all such
rights and claims against, every such incorporator, stockholder, officer or director as such, because of the creation of the indebtedness authorized by the Indenture, or under or by reason of the obligations, covenants or agreements contained in the
Indenture or in the Securities or implied therefrom, are hereby expressly waived and released as a condition of, and as a consideration for, the acceptance of the Securities. 

15.    Discharge of Indenture. The Indenture contains certain provisions pertaining to discharge and defeasance,
which provisions shall for all purposes have the same effect as if set forth herein. 
 16.    Authentication.
This Security shall not be valid until the Trustee signs the certificate of authentication attached to the other side of this Security. 

17.    Additional Amounts. The Company is obligated to pay Other Additional Amounts on this Security to the extent
provided in Section 10.03 of the Base Indenture. 
 18.    Abbreviations. Customary abbreviations may be
used in the name of a Securityholder or an assignee, such as: TEN COM(= tenants in common), TEN ENT (= tenants by the entireties), JT TEN(= joint tenants with right of survivorship and not as tenants in common), CUST (= Custodian), and U/G/M/A(=
Uniform Gifts to Minors Act). 
 19.    Governing Law. The Base Indenture, the Supplemental Indenture and this
Security shall be deemed to be a contract made under the internal laws of the State of New York, and for all purposes shall be construed in accordance with the laws of said State. 

  
 A-8 

 ASSIGNMENT FORM 

To assign this Security, fill in the form below: (I) or (we) assign and transfer this Security to 

 

	
	  

	(Insert assignee’s soc. sec. or tax I.D. no.)
	
	  

	  

	  

	  

	(Print or type assignee’s name, address and zip code)

 and irrevocably appoint
                                         
    agent to transfer this Security on the books of the Company. The agent may substitute another to act for him. 
 Date:
                                         
             
  

			
	 Your Signature
	 	 
	 (Sign exactly as your name appears on the face of this Security)

 Signature Guarantee:
                                         
                     

  
 A-9 

 OPTION OF HOLDER TO ELECT PURCHASE 

If you want to elect to have this Security purchased by the Company pursuant to Section 1.4(l) of the Supplemental Indenture, check the
box: 
  

	☐	 1.4(l) Change of Control Triggering Event 

If you want to elect to have only part of this Security purchased by the Company pursuant to Section 1.4(l) of the Supplemental
Indenture, state the amount:
€                                     

 

									
	Date:	 	  
	 	                            	  	 Your Signature
	 	  

		 		 		  	 (Sign exactly as your name appears on the face of this Security)

 Tax I.D. Number:
                                     

Signature Guarantee:
                                         
                                         
                            

(Signature must be guaranteed by a 

participant in a recognized signature 

guarantee medallion program) 

  
 A-10Exhibit 4.1

 

SUBSCRIPTION
AGREEMENT

 

SUBSCRIPTION
AGREEMENT (this “Agreement”) made as of the last date set forth on the signature page hereof between Avalon
GloboCare Corp., a Delaware corporation (the “Company”), and the undersigned (the “Subscriber”).

 

W
I T N E S S E T H:

 

WHEREAS,
the Company is conducting a private offering (the “Offering”) consisting of up to 645,161 shares (the “Shares”)
of common stock, $0.0001 par value per share (“Common Stock”), pursuant to Section 4(a)(2) of the Securities
Act of 1933, as amended (the “Securities Act”) and/or Rule 506 promulgated thereunder; and

 

WHEREAS,
the Subscriber desires to purchase that number of Shares set forth on the signature page hereof on the terms and conditions hereinafter
set forth.

 

NOW,
THEREFORE, in consideration of the premises and the mutual representations and covenants hereinafter set forth, the parties hereto
do hereby agree as follows:

 

		I.	SUBSCRIPTION
                                         FOR SHARES AND REPRESENTATIONS AND COVENANTS BY SUBSCRIBER

 

1.1Subject
to the terms and conditions hereinafter set forth, the Subscriber hereby irrevocably subscribes for and agrees to purchase from
the Company such number of Shares, and the Company agrees to sell to the Subscriber as is set forth on the signature page hereof,
at a per share price equal to $1.55 per Share. The purchase price is payable by check or by wire transfer of immediately
available funds pursuant to the Company’s wire instructions provided upon request. The date of the payment for the Shares by check
or by wire transfer shall be referred to as the Closing Date.

 

1.2 The
Subscriber recognizes that the purchase of the Shares involves a high degree of risk including, but not limited to, the following:
(a) the Company has limited operating history and requires substantial funds in addition to the proceeds of the Offering; (b)
an investment in the Company is highly speculative, and only investors who can afford the loss of their entire investment should
consider investing in the Company and the Shares; (c) the Subscriber may not be able to liquidate its investment; (d) transferability
of the Shares is extremely limited; (e) in the event of a disposition, the Subscriber could sustain the loss of its entire investment;
(f) the Company has not paid any dividends since its inception and does not anticipate paying any dividends; (g) the Company may
issue additional securities in the future which have rights and preferences that are senior to those of the Common Stock and (h)
the additional risk factors set forth on Exhibit A, which is attached hereto. Without limiting the generality of the representations
set forth in Section 1.5 below, the Subscriber represents that the Subscriber has carefully reviewed the risk factors described
in the Company’s filings made under the Securities Exchange Act of 1934, as amended.

 

    1

     

    

 

1.3
The Subscriber has carefully read the Form 8-Ks Current Report as filed with the Securities and Exchange Commission (the
“SEC”) on November 18, 2019, December 12, 2019, December 13, 2019, December 19, 2019, February 7, 2020 and
February 24, 2020 (the “2019 8Ks”), Form 10-Q Quarterly Report for the quarter ended September 30, 2019 as
filed with the SEC on November 14, 2019 and amended November 14, 2019 (the “10Q”) and the Form 10-K Annual
Report for the year ended December 31, 2018 as filed with the SEC on March 26, 2019 (“10K” together with
the 2019 8Ks and the 10Q, the “Reports”), which are attached hereto as Exhibit B, as well as all
other filings made by the Company with the SEC, and the additional risk factors (the “Risk Factors”),
which are attached hereto as Exhibit A and contained in the Reports. The Subscriber has been given the opportunity to ask
questions of, and receive answers from, the Company concerning the terms and conditions of this Offering, the Reports and the
Risk Factors and to obtain such additional information, to the extent the Company possesses such information or can acquire
it without unreasonable effort or expense, necessary to verify the accuracy of same as the Subscriber reasonably desires in
order to evaluate the investment. The Subscriber understands the Reports and the associated Risk Factors, and the Subscriber
has had the opportunity to discuss any questions regarding any of the disclosure in the Reports and the associated Risk
Factors with his counsel or other advisor. Notwithstanding the foregoing, the only information upon which the Subscriber has
relied is that set forth in the Reports and the associated Risk Factors. The Subscriber has received no representations or
warranties from the Company, its employees, agents or attorneys, in making this investment decision other than as set forth
in the Reports and the associated Risk Factors.

 

1.4 The
Subscriber represents that the Subscriber is an “accredited investor” as such term is defined in Rule 501 of Regulation
D (“Regulation D”) promulgated under the Securities Act, as indicated by the Subscriber’s responses to
the questions contained in Accredited Investor Questionnaire attached hereto as Exhibit C, and that the Subscriber is able
to bear the economic risk of an investment in the Shares.

 

1.5 The
Subscriber hereby acknowledges and represents that (a) the Subscriber has knowledge and experience in business and financial matters,
prior investment experience, including investment in securities that are non-listed, unregistered and/or not traded on a national
securities exchange nor on the NASDAQ, or the Subscriber has employed the services of a “purchaser representative”
(as defined in Rule 501 of Regulation D), attorney and/or accountant to read all of the documents furnished or made available
by the Company both to the Subscriber and to all other prospective investors in the Shares to evaluate the merits and risks of
such an investment on the Subscriber’s behalf; (b) the Subscriber recognizes the highly speculative nature of this investment;
and (c) the Subscriber is able to bear the economic risk that the Subscriber hereby assumes.

 

1.6 The
Subscriber hereby acknowledges receipt and careful review of this Agreement, including all exhibits thereto, and any documents
which may have been made available upon request as reflected therein (collectively referred to as the “Offering Materials”)
and hereby represents that the Subscriber has been furnished by the Company during the course of the Offering with all information
regarding the Company, the terms and conditions of the Offering and any additional information that the Subscriber has requested
or desired to know, and has been afforded the opportunity to ask questions of and receive answers from duly authorized officers
or other representatives of the Company concerning the Company and the terms and conditions of the Offering.

 

    2

     

    

 

1.7(a)In
making the decision to invest in the Shares the Subscriber has relied solely upon the information provided by the Company in the
Offering Materials. To the extent necessary, the Subscriber has retained, at its own expense, and relied upon appropriate professional
advice regarding the investment, tax and legal merits and consequences of this Agreement and the purchase of the Shares hereunder.
The Subscriber disclaims reliance on any statements made or information provided by any person or entity in the course of Subscriber’s
consideration of an investment in the Shares other than the Offering Materials.

 

(b) The
Subscriber represents that (i) the Subscriber was contacted regarding the sale of the Shares by the Company (or an authorized
agent or representative thereof) with whom the Subscriber had a prior substantial pre-existing relationship and (ii) no Shares
were offered or sold to it by means of any form of general solicitation or general advertising, and in connection therewith, the
Subscriber did not (A) receive or review any advertisement, article, notice or other communication published in a newspaper or
magazine or similar media or broadcast over television or radio, whether closed circuit, or generally available; or (B) attend
any seminar meeting or industry investor conference whose attendees were invited by any general solicitation or general advertising.

 

1.8 The
Subscriber hereby represents that the Subscriber, either by reason of the Subscriber’s business or financial experience
or the business or financial experience of the Subscriber’s professional advisors (who are unaffiliated with and not compensated
by the Company or any affiliate or selling agent of the Company, directly or indirectly), has the capacity to protect the Subscriber’s
own interests in connection with the transaction contemplated hereby.

 

1.9 The
Subscriber hereby acknowledges that the Offering has not been reviewed by the SEC nor any state regulatory authority since the
Offering is intended to be exempt from the registration requirements of Section 5 of the Securities Act, pursuant to Regulation
D. The Subscriber understands that the Shares have not been registered under the Securities Act or under any state securities
or “blue sky” laws and agrees not to sell, pledge, assign or otherwise transfer or dispose of the Shares unless they
are registered under the Securities Act and under any applicable state securities or “blue sky” laws or unless an
exemption from such registration is available.

 

1.10 The
Subscriber understands that the Shares have not been registered under the Securities Act by reason of a claimed exemption under
the provisions of the Securities Act that depends, in part, upon the Subscriber’s investment intention. In this connection,
the Subscriber hereby represents that the Subscriber is purchasing the Shares for the Subscriber’s own account for investment
and not with a view toward the resale or distribution to others. The Subscriber, if an entity, further represents that it was
not formed for the purpose of purchasing the Shares.

 

    3

     

    

 

1.11 The
Subscriber understands that the common stock issuable upon conversion of the Common Stock is quoted on the OTC Markets and that
there is a limited market for the Common Stock. The Subscriber understands that even if a public market develops for the Common
Stock, Rule 144 (“Rule 144”) promulgated under the Securities Act requires for non-affiliates, among other
conditions, a holding period prior to the resale (in limited amounts) of securities acquired in a non-public offering without
having to satisfy the registration requirements under the Securities Act. The Subscriber understands and hereby acknowledges that
the Company is under no obligation to register any of the Shares under the Securities Act or any state securities or “blue
sky” laws. The Subscriber understands that the Company must be current under the 1934 Act for the Subscriber to take advantage
of Rule 144.

 

1.12 Intentionally
Left Blank.

 

1.13 Lock-up
Agreement. The Subscriber agrees that it shall not transfer, offer, pledge, sell, contract to sell, grant any options for
the sale of, assign or otherwise dispose of, directly or indirectly, any of the Shares held by the Subscriber for a period of
one year from the Closing Date. If requested by an underwriter of Common Stock, Subscriber will reaffirm the agreement set forth
in this Section 1.13 in a separate writing in a form satisfactory to such underwriter. The Company may impose stop-transfer instructions
with respect to the Shares. The Subscriber may sell its shares in a private transaction only in the event that such purchaser
agrees to be bound by the terms of this Agreement including this Section 1.13.

 

1.14 The
Subscriber consents to the placement of a legend on any certificate or other document evidencing the Shares and any shares of
common stock issuable upon conversion of the Common Stock that such securities have not been registered under the Securities Act
or any state securities or “blue sky” laws and setting forth or referring to the restrictions on transferability and
sale thereof contained in this Agreement. The Subscriber is aware that the Company will make a notation in its appropriate records
with respect to the restrictions on the transferability of such Shares. The legend to be placed on each certificate shall be in
form substantially similar to the following:

 

“THE
SHARES REPRESENTED HEREBY HAVE NOT BEEN REGISTERED UNDER THE UNITED STATES SECURITIES ACT OF 1933, AS AMENDED (THE “ACT”)
OR ANY STATE SECURITIES OR “BLUE SKY LAWS,” AND MAY NOT BE OFFERED, SOLD, TRANSFERRED, ASSIGNED, PLEDGED OR HYPOTHECATED
ABSENT AN EFFECTIVE REGISTRATION THEREOF UNDER SUCH ACT OR COMPLIANCE WITH RULE 144 PROMULGATED UNDER SUCH ACT, OR UNLESS THE
COMPANY HAS RECEIVED AN OPINION OF COUNSEL, REASONABLY SATISFACTORY TO THE COMPANY AND ITS COUNSEL, THAT SUCH REGISTRATION IS
NOT REQUIRED.THE SHARES REPRESENTED BY THIS CERTIFICATE MAY NOT BE OFFERED, SOLD, PLEDGED, TRANSFERRED, ASSIGNED OR OTHERWISE
DISPOSED OF EXCEPT IN ACCORDANCE WITH THE REQUIREMENTS OF THAT CERTAIN SUBSCRIPTION AGREEMENT DATED AS OF DECEMBER __, 2019, A
COPY OF WHICH AGREEMENT THE COMPANY WILL FURNISH, WITHOUT CHARGE, TO THE HOLDER OF THIS CERTIFICATE UPON WRITTEN REQUEST THEREFOR.”

 

The
Company, at its discretion, may cause a stop transfer order to be placed with its transfer agent(s) with respect to the certificates
representing the Shares.

 

    4

     

    

 

1.15
The Subscriber understands that the Company will review this Agreement and is hereby given authority by the Subscriber to
call Subscriber’s bank or place of employment or otherwise review the financial standing of the Subscriber; and it is
further agreed that the Company, at its sole discretion, reserves the unrestricted right, without further documentation or
agreement on the part of the Subscriber, to reject or limit any subscription, to accept subscriptions for fractional Shares
and to close the Offering to the Subscriber at any time and that the Company will issue stop transfer instructions to its
transfer agent with respect to such Shares.

 

1.16 The
Subscriber hereby represents that the address of the Subscriber furnished by Subscriber on the signature page hereof is the Subscriber’s
principal residence if Subscriber is an individual or its principal business address if it is a corporation or other entity.

 

1.17 The
Subscriber represents that the Subscriber has full power and authority (corporate, statutory and otherwise) to execute and deliver
this Agreement and to purchase the Shares. This Agreement constitutes the legal, valid and binding obligation of the Subscriber,
enforceable against the Subscriber in accordance with its terms.

 

1.18 If
the Subscriber is a corporation, partnership, limited liability company, trust, employee benefit plan, individual retirement account,
Keogh Plan, or other tax-exempt entity, it is authorized and qualified to invest in the Company and the person signing this Agreement
on behalf of such entity has been duly authorized by such entity to do so.

 

1.19 The
Subscriber acknowledges that he, she or it are not Registered Representative of a FINRA member firm or a FINRA firm.

 

1.20
The Subscriber acknowledges that at such time, if ever, as the Shares are registered, sales of the Shares will be subject to
state securities laws.

 

1.21 The
Subscriber agrees not to issue any public statement with respect to the Subscriber’s investment or proposed investment in
the Company or the terms of any agreement or covenant between them and the Company without the Company’s prior written consent,
except such disclosures as may be required under applicable law or under any applicable order, rule or regulation.

 

1.22 The
Subscriber agrees to hold the Company and its directors, officers, employees, affiliates, controlling persons and agents and their
respective heirs, representatives, successors and assigns harmless and to indemnify them against all liabilities, costs and expenses
incurred by them as a result of (a) any sale or distribution of the Shares by the Subscriber in violation of the Securities Act
or any applicable state securities or “blue sky” laws; or (b) any false representation or warranty or any breach or
failure by the Subscriber to comply with any covenant made by the Subscriber in this Agreement (including the Confidential Investor
Questionnaire contained in Article VI herein) or any other document furnished by the Subscriber to any of the foregoing in connection
with this transaction.

 

    5

     

    

 

		II.	REPRESENTATIONS
                                         BY AND COVENANTS OF THE COMPANY

 

The
Company hereby represents and warrants to the Subscriber that:

 

2.1 Organization
and Qualification. The Company and each of its Subsidiaries, if any, is a corporation duly organized, validly existing and
in good standing under the laws of the jurisdiction in which it is incorporated, with full power and authority (corporate and
other) to own, lease, use and operate its properties and to carry on its business as and where now owned, leased, used, operated
and conducted. “Subsidiary” shall mean any corporation or other entity of which at least a majority of the
securities or other ownership interests having ordinary voting power (absolutely or contingently) for the election of directors
or other persons performing similar functions are at the time owned directly or indirectly by the Company and/or any of its other
Subsidiaries.

 

2.2 Capitalization.
The authorized, issued and outstanding capital stock of the Company is as set forth in the Reports.

 

2.3 Authorization;
Enforcement. The Company has all requisite corporate power and authority to enter into and perform this Agreement.

 

2.4 Acknowledgment
of Dilution. The Company understands and acknowledges the dilutive effect to the Common Stock upon the issuance of the Shares.

 

2.5 Bad
Actor Representation. None of the Company, any of its predecessors, any affiliated issuer, any director, executive officer,
other officer of the Company participating in the Offering, any beneficial owner of 20% or more of the Company’s outstanding
voting equity securities, calculated on the basis of voting power, nor any promoter (as that term is defined in Rule 405 under
the Securities Act) connected with the Company in any capacity at the time of sale (each, an “Issuer Covered Person”
and, together, “Issuer Covered Persons”) is subject to any of the “Bad Actor” disqualifications described
in Rule 506(d)(1)(i) to (viii) under the Securities Act (a “Disqualification Event”), except for a Disqualification
Event covered by Rule 506(d)(2) or (d)(3). The Company has exercised reasonable care to determine whether any Issuer Covered Person
is subject to a Disqualification Event.

 

2.6 Actions
Pending. Except as disclosed in the Reports, there is no action, suit, claim, investigation, arbitration, alternate dispute
resolution proceeding or any other proceeding pending or, to the knowledge of the Company, threatened against or involving the
Company, any Subsidiary (i) which questions the validity of this Agreement or any of the other Offering Materials or the transactions
contemplated hereby or thereby or any action taken or to be taken pursuant hereto or thereto or (ii) involving any of their respective
properties or assets. To the knowledge of the Company, there are no outstanding orders, judgments, injunctions, awards or decrees
of any court, arbitrator or governmental or regulatory body against the Company or any Subsidiary or any of their respective executive
officers or directors in their capacities as such.

 

2.7 Compliance
with Law. The Company and its Subsidiaries have all material franchises, permits, licenses, consents and other
governmental or regulatory authorizations and approvals necessary for the conduct of their respective business as now being
conducted by it unless the failure to possess such franchises, permits, licenses, consents and other governmental or
regulatory authorizations and approvals, individually or in the aggregate, could not reasonably be expected to have a
Material Adverse Effect.

 

    6

     

    

 

2.8 Compliance.
The Company: (i) is not in default under or in violation of (and no event has occurred that has not been waived that, with
notice or lapse of time or both, would result in a default by the Company), nor has the Company received notice of a claim
that it is in default under or that it is in violation of, any indenture, loan or credit agreement or any other agreement or
instrument to which it is a party or by which it or any of its properties is bound (whether or not such default or violation
has been waived), (ii) is in violation of any judgment, decree or order of any court, arbitrator or other governmental
authority or (iii) is or has been in violation of any statute, rule, ordinance or regulation of any governmental authority,
including without limitation all foreign, federal, state and local laws relating to taxes, environmental protection,
occupational health and safety, product quality and safety and employment and labor matters, except in each case as could not
have or reasonably be expected to result in a Material Adverse Effect.

 

2.9 No
Violation. The business of the Company and any Subsidiary is not being conducted in violation of any federal, state, local
or foreign governmental laws, or rules, regulations and ordinances of any governmental entity, except for possible violations
which singularly or in the aggregate could not reasonably be expected to have a Material Adverse Effect. The Company is not required
under federal, state, local or foreign law, rule or regulation to obtain any consent, authorization or order of, or make any filing
or registration with, any court or governmental agency in order for it to execute, deliver or perform any of its obligations under
the Offering Materials, or issue and sell the Common Stock in accordance with the terms hereof or thereof (other than (x) any
consent, authorization or order that has been obtained as of the date hereof, (y) any filing or registration that has been made
as of the date hereof or (z) any filings which may be required to be made by the Company with the SEC or state securities administrators
subsequent to each closing).

 

2.10 No
Conflicts. The execution, delivery and performance of this Agreement and the Offering Materials by the Company and the
consummation by the Company of the transactions contemplated herein and therein do not and will not (i) violate any provision
of the Articles or Bylaws, (ii) conflict with, or constitute a default (or an event which with notice or lapse of time or
both would become a default) under, or give to others any rights of termination, amendment, acceleration or cancellation of,
any agreement, mortgage, deed of trust, indenture, note, bond, license, lease agreement, instrument or obligation to which
the Company or any Subsidiary is a party or by which it or its properties or assets are bound, (iii) create or impose a lien,
mortgage, security interest, pledge, charge or encumbrance (collectively, “Lien”) of any nature on any
property of the Company or any Subsidiary under any agreement or any commitment to which the Company or any Subsidiary is a
party or by which the Company, or any Subsidiary is bound or by which any of its respective properties or assets are bound,
or (iv) result in a violation of any federal, state, local or foreign statute, rule, regulation, order, judgment or decree
(including federal and state securities laws and regulations) applicable to the Company or any Subsidiary or by which any
property or asset of the Company, or any Subsidiary are bound or affected, provided, however, that, excluded
from the foregoing in all cases are such conflicts, defaults, terminations, amendments, accelerations, cancellations and
violations as would not, individually or in the aggregate, have a Material Adverse Effect.

 

    7

     

    

 

2.11 Private
Placement and Solicitation. Assuming the accuracy of the Subscribers’ representations and warranties set forth in Section
1, no registration under the Securities Act is required for the offer and sale of the Common Stock by the Company to the Subscribers
as contemplated hereby. Based in part on the accuracy of the representations of the Subscribers in Section 1, and subject to timely
applicable Form D filings pursuant to Regulation D of the Securities Act with the SEC and pursuant to applicable state securities
laws, the offer, sale and issuance of the Common Stock to be issued pursuant to and in conformity with the terms of this Agreement,
will be issued in compliance with all applicable federal and state securities laws. Neither the Company nor any of its affiliates,
nor any person acting on its or their behalf, has engaged in any form of general solicitation or general advertising (within the
meaning of Regulation D under the Securities Act) in connection with the offer or sale of any of the Common Stock.

 

2.12 Governmental
Approvals. Except for the filing of any notice prior or subsequent to each closing that may be required under applicable state
and/or federal securities laws (which if required, shall be filed on a timely basis), including the filing of a Form D, no authorization,
consent, approval, license, exemption of, filing or registration with any court or governmental department, commission, board,
bureau, agency or instrumentality, domestic or foreign, is or will be necessary for, or in connection with, the execution or delivery
of the Common Stock, or for the performance by the Company of its obligations under this Agreement and the Offering Materials.

 

2.13 Investment
Company. The Company is not an “investment company” within the meaning of such term under the Investment
Company Act of 1940, as amended, and the rules and regulations of the SEC thereunder.

 

2.14 Use
of Proceeds. The Company shall use the proceeds from the sale of the Common Stock for working capital purposes and shall not,
directly or indirectly, use such proceeds for any distribution or dividend to any shareholder of the Company.

 

2.15 Securities
Compliance. The Company shall notify the SEC in accordance with its rules and regulations, of the transactions contemplated
by this Agreement and the Offering Materials, including filing a Form D with respect to the Common Stock, as required under Regulation
D and applicable “blue sky” laws if such Common Stock is offered pursuant to Rule 506 of Regulation D and shall take
all other necessary action and proceedings as may be required and permitted by applicable law, rule and regulation, for the legal
and valid issuance of the Common Stock to the Subscribers.

 

2.16 No
Integrated Offerings. The Company shall not make any offers or sales of any security (other than the securities being offered
or sold hereunder) under circumstances that would require registration of the securities being offered or sold hereunder under
the Securities Act.

 

    8

     

    

 

		III.	TERMS
                                         OF SUBSCRIPTION

 

3.1 All
funds shall be submitted directly to the Company’s account identified in Section 1.1 hereof.

 

3.2 Certificates
representing the Common Stock purchased by the Subscriber pursuant to this Agreement will be prepared for delivery to the Subscriber
within 15 business days following the closing, the timing of which is at the Company’s sole discretion, at which such purchase
takes place. The Subscriber hereby authorizes and directs the Company to deliver the certificates representing the Common Stock
purchased by the Subscriber pursuant to this Agreement directly to the Subscriber’s residential or business address indicated
on the signature page hereto.

 

		IV.	CONDITIONS
                                         TO OBLIGATIONS OF THE SUBSCRIBERS

 

4.1
The Subscriber’s obligation to purchase the Shares at the closing at which such purchase is to be consummated is
subject to the fulfillment on or prior to such closing of the following conditions, which conditions may be waived at the
option of each Subscriber to the extent permitted by law:

 

(a) Covenants.
All covenants, agreements and conditions contained in this Agreement to be performed by the Company on or prior to the date of
such closing shall have been performed or complied with in all material respects.

 

(b) No
Legal Order Pending. There shall not then be in effect any legal or other order enjoining or restraining the transactions
contemplated by this Agreement.

 

(c) No
Law Prohibiting or Restricting Such Sale. There shall not be in effect any law, rule or regulation prohibiting or restricting
such sale or requiring any consent or approval of any person, which shall not have been obtained, to issue the Shares (except
as otherwise provided in this Agreement).

 

		V.	MISCELLANEOUS

 

5.1 Any
notice or other communication given hereunder shall be deemed sufficient if in writing and sent by registered or certified mail,
return receipt requested, or delivered by hand against written receipt therefor, addressed as follows:

 

if
to the Company, to it at:

 

Avalon GloboCare Corp.

4400 Route 9 South, Suite 3100

Freehold, New Jersey 07728

Attention: David Jin

Telephone: 

Facsimile:     

 

with
a copy to: 

 

Fleming
PLLC 

30
Wall Street, 8th Floor 

New
York, New York 10005 

Attention:
Stephen Fleming 

Telephone:
(516) 833-5034 

Facsimile:
(516) 977-1209 

Email:
smf@flemingpllc.com

 

if
to the Subscriber, to the Subscriber’s address indicated on the signature page of this Agreement.

 

    9

     

    

 

Notices
shall be deemed to have been given or delivered on the date of mailing, except notices of change of address, which shall be deemed
to have been given or delivered when received.

 

5.2
Except as otherwise provided herein, this Agreement shall not be changed, modified or amended except by a writing signed by
the parties to be charged, and this Agreement may not be discharged except by performance in accordance with its terms or by
a writing signed by the party to be charged.

 

5.3
Subject to the provisions of Section 5.10, this Agreement shall be binding upon and inure to the benefit of the parties
hereto and to their respective heirs, legal representatives, successors and assigns. This Agreement sets forth the entire
agreement and understanding between the parties as to the subject matter hereof and merges and supersedes all prior
discussions, agreements and understandings of any and every nature among them.

 

5.4 Upon
the execution and delivery of this Agreement by the Subscriber, this Agreement shall become a binding obligation of the Subscriber
with respect to the purchase of Shares as herein provided, subject, however, to the right hereby reserved by the Company to enter
into the same agreements with other subscribers and to add and/or delete other persons as subscribers.

 

5.5 NOTWITHSTANDING
THE PLACE WHERE THIS AGREEMENT MAY BE EXECUTED BY ANY OF THE PARTIES HERETO, THE PARTIES EXPRESSLY AGREE THAT ALL THE TERMS AND
PROVISIONS HEREOF SHALL BE CONSTRUED IN ACCORDANCE WITH AND GOVERNED BY THE LAWS OF THE STATE OF NEW JERSEY WITHOUT REGARD TO
SUCH STATE’S PRINCIPLES OF CONFLICTS OF LAW. IN THE EVENT THAT A JUDICIAL PROCEEDING IS NECESSARY, THE SOLE FORUM FOR RESOLVING
DISPUTES ARISING OUT OF OR RELATING TO THIS AGREEMENT IS THE COURTS STATE OF NEW JERSEY IN AND FOR THE COUNTY OF MONMOUTH OR THE
FEDERAL COURTS FOR SUCH STATE AND COUNTY, AND ALL RELATED APPELLATE COURTS, THE PARTIES HEREBY IRREVOCABLY CONSENT TO THE JURISDICTION
OF SUCH COURTS AND AGREE TO SAID VENUE.

 

5.6
In order to discourage frivolous claims the parties agree that unless a claimant in any proceeding arising out of this
Agreement succeeds in establishing his claim and recovering a judgment against another party (regardless of whether such
claimant succeeds against one of the other parties to the action), then the other party shall be entitled to recover from
such claimant all of its/their reasonable legal costs and expenses relating to such proceeding and/or incurred in preparation
therefor.

 

    10

     

    

 

5.7
The holding of any provision of this Agreement to be invalid or unenforceable by a court of competent jurisdiction shall not
affect any other provision of this Agreement, which shall remain in full force and effect. If any provision of this Agreement
shall be declared by a court of competent jurisdiction to be invalid, illegal or incapable of being enforced in whole or in
part, such provision shall be interpreted so as to remain enforceable to the maximum extent permissible consistent with
applicable law and the remaining conditions and provisions or portions thereof shall nevertheless remain in full force and
effect and enforceable to the extent they are valid, legal and enforceable, and no provisions shall be deemed dependent upon
any other covenant or provision unless so expressed herein.

 

5.8
It is agreed that a waiver by either party of a breach of any provision of this Agreement shall not operate, or be construed,
as a waiver of any subsequent breach by that same party.

 

5.9  The
parties agree to execute and deliver all such further documents, agreements and instruments and take such other and further action
as may be necessary or appropriate to carry out the purposes and intent of this Agreement.

 

5.10
This Agreement may be executed in two or more counterparts each of which shall be deemed an original, but all of which shall
together constitute one and the same instrument.

 

5.11
Nothing in this Agreement shall create or be deemed to create any rights in any person or entity not a party to this Agreement.

 

[REMAINDER
OF PAGE INTENTIONALLY LEFT BLANK]

 

    11

     

    

 

NUMBER
OF SHARES 645,161 X $1.55 = $1,000,000 (the “Purchase Price”) 

 

	 	 	 
	Signature	 	Signature
    (if purchasing jointly)
	 	 	 
	 	 	 
	Name Typed or Printed	 	Name Typed or Printed
	 	 	 
	 	 	 
	Title (if Subscriber
    is an Entity)	 	Title (if Subscriber is an Entity)
	 	 	 
	 	 	 
	Entity Name (if
    applicable)	 	Entity Name (if applicable)
	 	 	 
	 	 	 
	 	 	 
	 	 	 
	Address	 	Address
	 	 	 
	 	 	 
	City, State and
    Zip Code	 	City, State and
    Zip Code
	 	 	 
	 	 	 
	Telephone-Business	 	Telephone-Business
	 	 	 
	 	 	 
	Telephone-Residence	 	Telephone-Residence
	 	 	 
	 	 	 
	Facsimile-Business	 	Facsimile-Business
	 	 	 
	 	 	 
	Facsimile-Residence	 	Facsimile-Residence
	 	 	 
	 	 	 
	Tax ID # or Social
    Security #	 	Tax ID # or Social Security #

 

Name
in which securities should be issued:_________________________________ 

 

Dated:April
__, 2020 

 

This
Subscription Agreement is agreed to and accepted as of April __, 2020.

 

		AVALON GLOBOCARE CORP.
	 	 	 
	 	By:	               
	 	Name:	 
	 	Title:	 

 

    12

     

    

CERTIFICATE OF SIGNATORY 

 

(To
be completed if Securities are

being
subscribed for by an entity)

 

I,
____________________________, am the ____________________________ of 

 

__________________________________________
(the “Entity”). 

 

I
certify that I am empowered and duly authorized by the Entity to execute and carry out the terms of the Subscription Agreement
and to purchase and hold the shares of Common Stock, and certify further that the Subscription Agreement has been duly and validly
executed on behalf of the Entity and constitutes a legal and binding obligation of the Entity. 

 

IN
WITNESS WHEREOF, I have set my hand this ________ day of _________________, 2019 

 

_______________________________________

 

(Signature)

 

    13

     

    

 

 

Exhibit
A - Risk Factors

 

The
offering price for the Common Stock has been determined by the Company. 

 

The
price at which the Common Stock is being offered has been determined by us based on current sales, sales forecasts and standard
corporate valuation estimation methods. There is no direct relationship between the offering price and our assets, book value,
net worth, or any other economic or recognized criteria of value.

 

An
investment in the Shares is speculative and there can be no assurance of any return on any such investment. 

 

An
investment in the Shares is speculative and there is no assurance that investors will obtain any return on their investment. Investors
will be subject to substantial risks involved in an investment in the Company, including the risk of losing their entire investment.

 

We
have significant discretion over certain of the net proceeds. 

 

The
maximum gross proceeds to us from the sale of the Shares will be $2,000,000. The net proceeds of this Offering will be applied
to general corporate purposes, including, but not limited to employee/consultant salaries and fees, professional fees, and working
capital. The use of proceeds may change as management deems fit. The proceeds shall be used to carry out our business plan, and
satisfy all our expenses, foreseeable and unforeseeable. As is the case with any business, particularly one without a proven business
model, it should be expected that certain expenses unforeseeable to management at this juncture will arise in the future. There
can be no assurance that management’s use of proceeds generated through this Offering will prove optimal or translate into
revenue or profitability for the Company. Investors are urged to consult with their attorneys, accountants and personal investment
advisors prior to making any decision to invest in the Company. 

 

The
Maximum Offering will be offered by on a “Best Efforts” basis, and we may not raise the Maximum offering. 

 

We
are offering the shares with respect to the Maximum Offering. In a best efforts offering such as the one described in this Memorandum,
there is no assurance that we will sell the Maximum Offering. Accordingly, we may close upon amounts less than the Maximum Offering
which may not provide us with sufficient funds to fully implement our business plan.

 

    14

     

    

 

Exhibit
B - SEC Reports

 

 

 

 

 

 

    15

     

    

 

Exhibit
C - Accredited Investor Questionnaire 

 

CONFIDENTIAL
INVESTOR QUESTIONNAIRE

 

1. The
Subscriber represents and warrants that he, she or it comes within one category marked below, and that for any category marked,
he, she or it has truthfully set forth, where applicable, the factual basis or reason the Subscriber comes within that category.
ALL INFORMATION IN RESPONSE TO THIS SECTION WILL BE KEPT STRICTLY CONFIDENTIAL. The undersigned agrees to furnish any additional
information which the Company deems necessary in order to verify the answers set forth below.

 

	Category A __	The undersigned is an individual
    (not a partnership, corporation, etc.) whose individual net worth, or joint net worth with his or her spouse, presently exceeds
    $1,000,000. 
	 	 
	 	Explanation. In
    calculating net worth you may include equity in personal property and real estate (excluding your principal residence), cash,
    short-term investments, stock and securities. Equity in personal property and real estate should be based on the fair market
    value of such property less debt secured by such property.
	 	 
	Category B __	The undersigned
    is an individual (not a partnership, corporation, etc.) who had an income in excess of $200,000 in each of the two most recent
    years, or joint income with his or her spouse in excess of $300,000 in each of those years (in each case including foreign
    income, tax exempt income and full amount of capital gains and losses but excluding any income of other family members and
    any unrealized capital appreciation) and has a reasonable expectation of reaching the same income level in the current year. 
	 	 
	Category
    C __	The
    undersigned is a director or executive officer of the Company which is issuing and selling the Shares.
	 	 
	Category D __	The undersigned is a bank; a savings
    and loan association; insurance company; registered investment company; registered business development company; licensed
    small business investment company (“SBIC”); or employee benefit plan within the meaning of Title 1 of ERISA and
    (a) the investment decision is made by a plan fiduciary which is either a bank, savings and loan association, insurance company
    or registered investment advisor, or (b) the plan has total assets in excess of $5,000,000 or (c) is a self directed plan
    with investment decisions made solely by persons that are accredited investors. (describe entity)
	 	 
	 	 
	 	 
	Category E __	The undersigned
    is a private business development company as defined in section 202(a) (22) of the Investment Advisors Act of 1940. (describe
    entity)
	 	 
	 	 
	 	 

 

    16

     

    

 

	

Category
F __

	The
    undersigned is either a corporation, partnership, Massachusetts business trust, or non-profit organization within the meaning
    of Section 501(c) (3) of the Internal Revenue Code, in each case not formed for the specific purpose of acquiring the Shares
    and with total assets in excess of $5,000,000. (describe entity)
	 	 
	 	 
	 	 
	Category G __	The undersigned
    is a trust with total assets in excess of $5,000,000, not formed for the specific purpose of acquiring the Shares, where the
    purchase is directed by a “sophisticated investor” as defined in Regulation 506(b)(2)(ii) under the Act.
	 	 
	Category H __	The
                                         undersigned is an entity (other than a trust) in which all of the equity owners are “accredited
                                         investors” within one or more of the above categories. If relying upon this Category
                                         alone, each equity owner must complete a separate copy of this Agreement. (describe entity)

	 	 
	 	 
	 	 
	Category I __	The undersigned
    is not within any of the categories above and is therefore not an accredited investor.
	 	 
	 	The undersigned
    agrees that the undersigned will notify the Company at any time on or prior to the closing in the event that the representations
    and warranties in this Agreement shall cease to be true, accurate and complete.

 

2.       SUITABILITY
(please answer each question)

 

(a)           For
an individual Subscriber, please describe your current employment, including the company by which you are employed and its principal
business: 

 

___________________________________________________________________ 

___________________________________________________________________

___________________________________________________________________

 

(b)           For
an individual Subscriber, please describe any college or graduate degrees

held by you: 

___________________________________________________________________

___________________________________________________________________

___________________________________________________________________

___________________________________________________________________

 

(c)           For
all Subscribers, please list types of prior investments: 

 

___________________________________________________________________

___________________________________________________________________

___________________________________________________________________

___________________________________________________________________

 

    17

     

    

 

(d)          For
all Subscribers, please state whether you have participated in other private placements before:

 

YES_______NO_______

 

(e)           If
your answer to question (d) above was “YES”, please indicate frequency of such prior participation in private placements
of:

 

	 	 	 Public

        Companies
	 	

        Private

        Companies
	 	Public
                                         or Private Companies

        with
        no, or insignificant,

        assets
        and operations

	Frequently	 		 		 	
	Occasionally	 		 		 	
	Never	 		 		 	

  

(f)           For
individual Subscribers, do you expect your current level of income to significantly decrease in the foreseeable future:

 

YES_______NO_______

 

(g)           For
trust, corporate, partnership and other institutional Subscribers, do you expect your total assets to significantly decrease in
the foreseeable future:

 

YES_______NO_______

 

(h)          For
all Subscribers, do you have any other investments or contingent liabilities which you reasonably anticipate could cause you to
need sudden cash requirements in excess of cash readily available to you:

 

YES_______NO_______

 

(i)            For
all Subscribers, are you familiar with the risk aspects and the non-liquidity of investments such as the securities for which
you seek to subscribe?

 

YES_______NO_______

 

(j)          
For all Subscribers, do you understand that there is no guarantee of financial return on this investment and that you run the
risk of losing your entire investment?

 

YES_______NO_______

 

    18

     

    

 

3.       MANNER
IN WHICH TITLE IS TO BE HELD. (circle one)

 

(a)       Individual
Ownership 

(b)       Community
Property 

(c)       Joint
Tenant with Right of Survivorship (both parties must sign) 

(d)       Partnership* 

(e)       Tenants
in Common 

(f)       Company* 

(g)       Trust* 

(h)       Other*

 

*If
Securities are being subscribed for by an entity, the attached Certificate of Signatory must also be completed.

 

The
undersigned is informed of the significance to the Company of the foregoing representations and answers contained in the Confidential
Investor Questionnaire contained in this Article VI and such answers have been provided under the assumption that the Company
will rely on them.

 

 

 19

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