Document:

Exhibit 10.49

                                SECOND ALLONGE TO
                           CONVERTIBLE REVOLVING NOTE

This  modification  made this  10th day of  September,  2001 to the  Convertible
Revolving  Note dated June 10, 1999,  as amended,  payable to the order of FLEET
NATIONAL  BANK,  SUCCESSOR IN INTEREST TO SUMMIT BANK ("Note") and to which Note
these presents are so firmly affixed as to become a part thereof.

Notwithstanding  anything to the contrary set forth in the Note,  all references
to "Base Rate" are hereby  amended to refer to the "Prime Rate." The  definition
of "Base Rate" is deleted and replaced with the  following  definition of "Prime
Rate:" The Prime Rate of Lender  means the  fluctuating  Prime Rate of  interest
established  by Fleet  National  Bank from time to time whether or not such rate
shall be otherwise published.  The Prime Rate is established for the convenience
of Lender.  It is not necessarily  Lender's lowest rate. In the event that there
should be a change in the Prime Rate of Lender,  such change  shall be effective
on the date of such change without notice to Borrower or any guarantor, endorser
or surety. Any such change will not effect or alter any other term or conditions
of this Note.

Except as specifically  modified herein,  all of the terms and conditions of the
Note shall remain in full force and effect and any term in initial  capitals and
not  otherwise  defined  herein shall have the meaning  ascribed  thereto in the
Note.

Witness:                                      OSTEOTECH, INC.
                                                  A Delaware Corporation

/s/ Mark H. Burroughs                         By: /s/ Michael J. Jeffries
---------------------                             -------------------------
                                                  MICHAEL J. JEFFRIES
                                                  Executive Vice President

Witness:                                      OSTEOTECH INVESTMENT
                                                CORPORATION
                                                A New Jersey Corporation

/s/ Mark H. Burroughs                         By: /s/ Michael J. Jeffries
---------------------                             -------------------------
                                                  MICHAEL J. JEFFRIES
                                                  Executive Vice President

Witness:                                       CAM IMPLANTS, INC.
                                                 A Colorado Corporation

/s/ Mark H. Burroughs                         By: /s/ Michael J. Jeffries
---------------------                             -------------------------
                                                  MICHAEL J. JEFFRIES
                                                  Chief Financial Officer

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<PAGE>

Signatures continued ......

............................  continuation  of  signatures  to Second  Allonge to
Convertible Revolving Note

Witness:                                        OSTEOTECH, B.V.
                                                A Company of The Netherlands

/s/ Mark H. Burroughs                           By: /s/ Michael J. Jeffries
---------------------                               -------------------------
                                                    MICHAEL J. JEFFRIES
                                                    Managing Director

Witness:                                        H.C. IMPLANTS, B.V.
                                                A Company of The Netherlands

/s/ Mark H. Burroughs                           By: /s/ Michael J. Jeffries
---------------------                               -------------------------
                                                    MICHAEL J. JEFFRIES
                                                    Managing Director

Witness:                                        CAM IMPLANTS, B.V.
                                                A Company of The Netherlands

/s/ Mark H. Burroughs                           By: /s/ Michael J. Jeffries
---------------------                               -------------------------
                                                    MICHAEL J. JEFFRIES
                                                    Managing Director

Witness:                                        OSTEOTECH/CAM SERVICES, B.V.
                                                A Company of The Netherlands

/s/ Mark H. Burroughs                           By: /s/ Michael J. Jeffries
---------------------                               -------------------------
                                                    MICHAEL J. JEFFRIES
                                                    Managing Director

Witness:                                        OST DEVELOPPEMENT
                                                A Corporation of France

/s/ Mark H. Burroughs                           By: /s/ Michael J. Jeffries
---------------------                               -------------------------
                                                    MICHAEL J. JEFFRIES
                                                    Managing Director

                                                                           E-156Exhibit 10.50

                    SEPARATION AGREEMENT AND GENERAL RELEASE

     This  Agreement  is  executed  on the dates set forth  below by and between
ARTHUR A. ALFARO ("Alfaro"),  residing at 167 Stone Hill, Colts Neck, New Jersey
07722, and OSTEOTECH,  INC.  ("Osteotech"),  located at 51 James Way, Eatontown,
New Jersey 07724.

                              W I T N E S S E T H:
                              - - - - - - - - - -

     WHEREAS,   Alfaro  and  Osteotech  disagree  concerning  the  circumstances
surrounding the termination of Alfaro's employment with Osteotech; and

     WHEREAS, Alfaro and Osteotech desire to resolve this disagreement and other
disputes that have arisen between them;

     NOW, in consideration  of the mutual covenants and obligations  hereinafter
set forth, Alfaro and Osteotech agree as follows:

     1.  Alfaro and  Osteotech  confirm  (i) the  separation  of Alfaro from his
employment  at Osteotech  upon the close of business on January 15,  2002,  (ii)
Alfaro's  resignation  as President  and Chief  Operating  Officer of Osteotech,
effective  November 15, 2001,  and (iii)  Alfaro's  resignation as a Director of
Osteotech, effective January 15, 2002.

     2.  Osteotech  shall pay  Alfaro an amount  equal to ten months of his base
salary  in the sum of two  hundred  fifty  two  thousand  five  hundred  dollars
($252,500.00).  Such payment shall be made by check in twenty (20)  semi-monthly
installments, between January 15, 2002 and November 15, 2002, each in the amount
of twelve thousand six hundred twenty five dollars  ($12,625.00) gross,  subject
to  applicable  deductions  for  taxes.  Alfaro  shall  also  receive as soon as
possible in calendar year 2002, the amount of eleven  thousand two hundred fifty
dollars  ($11,250.00)  gross,  subject to applicable  deductions  for taxes.  In
addition,  if  Alfaro  has

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not as of November 15, 2002 obtained comparable employment (as evidenced by, for
example,  duties and  responsibilities or overall  compensation and/or potential
compensation  or position  with the new company or other  entity),  Alfaro shall
receive semi-monthly  payments each in the amount of twelve thousand six hundred
twenty five dollars  ($12,625.00)  gross,  subject to applicable  deductions for
taxes,  during the period  between  November  15, 2002 and  November 15, 2003 or
until Alfaro obtains  comparable  employment,  whichever  occurs sooner.  In the
event  that  Alfaro  does  not  obtain   comparable   employment   but  receives
compensation  in any  form  from  any  other  source  of  employment  (including
consulting or self-employment)  between November 15, 2002 and November 15, 2003,
all such  compensation  shall be deducted from any amounts that may otherwise be
owed by  Osteotech  to Alfaro as set forth  herein  during such  period.  Alfaro
agrees to promptly  notify Mr. Richard W. Bauer,  President and Chief  Executive
Officer  of  Osteotech,  or his  successor  if and  when he  obtains  comparable
employment  between  November 15, 2002 and November 15, 2003.  Such notice shall
state  the date  such  comparable  employment  commenced.  In the  event  Alfaro
receives  compensation  between November 15, 2002 and November 15, 2003 from any
source of employment that is not comparable employment ("Alternate Employment"),
he shall notify Mr. Bauer or his  successor of the amount of gross  compensation
from Alternate  Employment  received by Alfaro during each month of such period.
Such notification shall be delivered to Mr. Bauer or his successor no later than
the tenth  (10th)  day of the  month  immediately  following  the month in which
compensation  from  Alternate  Employment  is received by Alfaro.  The amount of
gross  compensation  payable to Alfaro by Osteotech  hereunder  during the month
immediately  following the month in which compensation from Alternate Employment
is received by Alfaro (or subsequent months to the

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<PAGE>

extent required to provide  Osteotech full credit hereunder) shall be reduced by
the gross amount of such  compensation  from  Alternate  Employment  received by
Alfaro.

     3. (a)  Consistent  with past  practice  including the payment of premiums,
Alfaro's  participation  in  Osteotech's  Medical/Dental  Plan,  or  Osteotech's
payment of premiums  for  Alfaro's  family  medical and dental  coverage,  shall
continue  through  the  earlier  of (i) the end of the last pay period for which
payments are being made as described in Section 2 of this Agreement, or (ii) the
date that Alfaro obtains comparable employment.

     (b)  Consistent  with past  practice  including  the  payment of  premiums,
Alfaro's  participation  in Osteotech's  life insurance  coverage shall continue
through the earlier of (i) the end of the last pay period for which payments are
being made as  described in Section 2 of this  Agreement,  or (ii) the date that
Alfaro obtains comparable employment.

     (c) Alfaro shall be eligible to receive reasonable  outplacement  benefits,
consistent with those previously provided by Osteotech to departing  management,
as long as he is actively  seeking new  employment  following his  employment by
Osteotech,  which benefits must be approved by and arranged  through Mr. Charles
Jannetti, Director of Human Resources of Osteotech, or his successor.

     (d) Alfaro's  participation  in  Osteotech's  Stock Purchase Plan shall end
effective  January 15,  2002.  Alfaro  confirms  that he never  participated  in
Osteotech's 401(k) Plan and that he is not entitled to any benefits thereunder.

     4. Alfaro agrees that no additional  compensation of any kind shall be paid
to him, and the benefits  provided to him under this Agreement  shall be in full
payment and  satisfaction  of any and all financial  obligations due to him from
Osteotech. Alfaro shall not be entitled to receive any bonus based on individual
performance  or  otherwise.  It is  understood  and

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agreed  that,  except for the 100,000 and 25,000  vested  options  described  in
Exhibits A and B hereto,  respectively,  none of the  options  granted to Alfaro
under any of  Osteotech's  option  plans had vested as of January 15,  2002.  In
accordance  with their  terms,  all such  unvested  options,  including  without
limitation those unvested options  described in Exhibits A and B, terminated and
were of no further  force or effect as of January 15, 2002.  The vested  options
described in Exhibits A and B will be exercisable by Alfaro until April 15, 2002
(90 days after  January 15, 2002),  subject to the terms and  conditions of such
options.

     5. All inquiries received by Osteotech  concerning Alfaro from potential or
future  employers shall be directed to Mr. Charles  Jannetti,  or his successor,
for response.  Should any potential or future employer  contact  Osteotech for a
reference concerning Alfaro,  Osteotech shall verify only Alfaro's job title and
dates of employment.

     6. (a) It is  understood  and agreed that,  by this  Agreement,  Alfaro and
Osteotech  intend to settle  any and all  claims  which  Alfaro  has or may have
against  Osteotech  arising out of or  resulting  from  Alfaro's  employment  at
Osteotech and his separation from such employment.  Accordingly, in exchange for
the benefits  provided to Alfaro by this  Agreement,  Alfaro,  for himself,  his
heirs,  successors  and  assigns,  hereby  voluntarily  discharges  and releases
Osteotech  and  its  affiliates,  parent  and  subsidiary  companies,  officers,
directors,  employees,  agents,  representatives,  successors  and assigns  (the
"Releasees")  from any and all claims or liabilities of any kind or description,
known or unknown,  suspected or unsuspected,  fixed or contingent,  which Alfaro
ever had, now has or hereafter  may have against each or any of the Releasees by
reason of any  matter  whatsoever  arising  out of or  resulting  from  Alfaro's
employment at Osteotech and his separation from such employment. This release of
claims   specifically   includes,   but  is  not   limited   to,  any  claim  of
discrimination,  including  any claim

                                                                           E-160
<PAGE>

arising under, or based upon, the Age  Discrimination  in Employment Act (or the
Older  Workers  Benefit  Protection  Act),  Title VII of the Civil Rights Act of
1964,  the  Americans  With  Disabilities  Act and/or the New Jersey Law Against
Discrimination,  and any and all  contract,  quasi-contract,  estoppel,  tort or
statutory  claims under federal,  state or local law arising out of or resulting
from Alfaro's employment at Osteotech and his separation from such employment.

     (b) Nothing  contained in this Agreement shall be deemed to be a release of
any obligation undertaken in this Agreement.

     7. In further  recognition  of the  benefits  provided to Alfaro under this
Agreement, Alfaro agrees to consult with Osteotech with respect to matters which
occurred during the period of Alfaro's employment at Osteotech, and to cooperate
with Osteotech in connection with the defense of any claim or proceeding made or
filed against  Osteotech (or any officer,  director or employee  thereof) or the
prosecution of any claim or proceeding made or filed by Osteotech,  in each case
relating  to  Osteotech's  business  during  the  period  of his  employment  at
Osteotech.  Alfaro agrees to make himself available at reasonable times and upon
reasonable  notice,  consistent  with  his  other  business  commitments,  to be
interviewed or deposed  concerning any such claim or proceeding.  Alfaro further
agrees to promptly notify Mr. Richard W. Bauer, or his successor or designee, if
Alfaro receives any legal notices or requests for information from any person or
entity, other than a representative of Osteotech, concerning matters which arose
during the period of his employment at Osteotech.  Osteotech agrees to reimburse
Alfaro  for  reasonable  and  necessary   out-of-pocket   expenses  incurred  in
connection with the satisfaction of his obligations under this section. However,
Alfaro shall seek advance approval from Mr. Richard W. Bauer or his successor or
designee before  incurring any  significant  expense for which Alfaro shall seek
reimbursement from Osteotech.

                                                                           E-161
<PAGE>

     8. Alfaro agrees that he shall not make any public  disparaging  statements
concerning (a) Osteotech; (b) any officer, director or employee of Osteotech; or
(c) Osteotech's  products or services.  Osteotech agrees that it, as well as its
officers  and  directors,  shall  not make  any  public  disparaging  statements
concerning Alfaro. Alfaro agrees that Alfaro's violation of his obligation under
this Section 8 shall  entitle  Osteotech to cease making any of the payments and
providing  any other  benefits to Alfaro under this  Agreement  from the date of
such violation,  and entitle Osteotech to seek injunctive relief against Alfaro.
Osteotech  agrees  that  Osteotech's  violation  of its  obligations  under this
Section 8 will only entitle Alfaro to seek injunctive relief against Osteotech.

     9. (a) Alfaro will not disclose or provide to any person, firm, corporation
or entity  (except when  authorized  by  Osteotech in writing) any  information,
materials,  biologics or animals which are owned by Osteotech or which came into
the  possession  of  Osteotech  from  a  third  party  under  an  obligation  of
confidentiality,  including without  limitation,  information  relating to trade
secrets,  business  methods,  products,  processes,  procedures,  development or
experimental  projects,  suppliers,  customer lists or the needs of customers or
prospective customers,  clients, etc. (collectively "Confidential Information"),
which Confidential  Information came into his possession or knowledge during the
course of his employment by Osteotech, and Alfaro will not use such Confidential
Information  for his  own  purpose  or for  the  purpose  of any  person,  firm,
corporation  or entity,  other than  Osteotech.  The  provisions of this section
shall not apply to the  following  Confidential  Information:  (i)  Confidential
Information  which at the time of  disclosure  is already in the public  domain;
(ii) Confidential Information which Alfaro can demonstrate was in his possession
or known to him prior to the  effective  date of his  employment  by  Osteotech;
(iii)  Confidential  Information which  subsequently  becomes

                                                                           E-162
<PAGE>

part of the  public  domain  through  no  fault  of  Alfaro;  (iv)  Confidential
Information  which becomes known to Alfaro through a third party who is under no
obligation of  confidentiality  to Osteotech;  and (v) Confidential  Information
which is  required  to be  disclosed  by law or by  judicial  or  administrative
proceedings.

     (b) Alfaro agrees that, no later than January 31, 2002, he shall deliver to
Mr. Charles Jannetti of Osteotech all books, records, notes, documents and other
written or computer  generated  materials of any nature  whatsoever  relating to
Osteotech's  business  and any other  Osteotech  property in his  possession  or
within his control (e.g., credit cards, equipment, office keys, samples). Alfaro
agrees that he shall not keep in his  possession  or control any of  Osteotech's
property of any kind.

     (c) For the period of time  Alfaro  receives  payments as set forth in this
Agreement,  Alfaro agrees that he shall not directly or indirectly be engaged in
or assist  others in engaging in any  business or activity  which is involved in
selling  products,  processes or services  which  compete  with any  significant
product, process or service which Osteotech is developing,  marketing or selling
at the time of such  termination,  whether his involvement  shall be as an owner
(except for passive  ownership of up to five percent (5%) of the securities of a
company),  officer,  director,  employee,  consultant,  partner  or  agent.  For
purposes of this provision,  products,  processes or services which Osteotech is
marketing or selling shall be deemed  "significant"  if sales of such  products,
processes or services exceed ten percent (10%) of Osteotech's total sales.

     (d) Alfaro agrees that the restrictions  imposed upon him by this Agreement
are  reasonable  and  that  they  are   appropriate  and  necessary  to  protect
Osteotech's

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legitimate business  interests.  Alfaro further agrees that such restrictions do
not and will not impose an undue hardship upon him.

     (e) Alfaro agrees that the post-termination  obligations  undertaken by him
pursuant to this Agreement are consistent with those  undertaken by him pursuant
to his Employment  Agreement (the "Employment  Agreement"),  dated September 13,
1999. Alfaro further acknowledges and agrees that the terms set forth in section
2 of this  Agreement  reflect  a  modification  of the  terms  set  forth in the
Employment   Agreement.   Alfaro  further   agrees  that  the   post-termination
obligations  contained  in the  Employment  Agreement  remain in full  force and
effect.

     (f) Alfaro agrees that in the event of a breach or threatened breach by him
of the  provisions  of this  section,  in whole or in part,  Osteotech  shall be
irreparably  damaged and shall be entitled to (1) an injunction  from a court of
competent  jurisdiction  to prevent any further  breaches,  and (2)  recovery of
costs and reasonable  attorney's  fees incurred by Osteotech in connection  with
its application for injunctive relief. Nothing contained in this Agreement shall
be construed as prohibiting Osteotech from pursuing any other remedies which may
be available  for such breach or  threatened  breach,  including the recovery of
damages from Alfaro.

     10. Alfaro  acknowledges  and  represents  that he fully  understands  this
Agreement,  that he has had adequate and  reasonable  opportunity  to review the
Agreement, that he was advised to consult with independent counsel of his choice
before signing it, that he did in fact consult with  independent  counsel of his
choice before signing it, and that he is signing it voluntarily.

     11. The parties  acknowledge  that as a public  company  Osteotech  will be
required to file a copy of this Agreement as an exhibit to its periodic  reports
filed with the

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Securities and Exchange Commission (the "SEC") and to describe the terms of this
Agreement in such periodic reports.  Osteotech shall promptly advise Alfaro when
it has filed this  Agreement  with the SEC. Until such time as this Agreement is
filed with the SEC,  Alfaro agrees not to discuss its terms and conditions  with
anyone but his immediate  family members,  his attorney and/or his other outside
consultants or professionals who have a need to know the terms and conditions of
this  Agreement,  or as  otherwise  required  by law.  Alfaro  agrees  that  the
disclosure  by him or on his behalf,  prior to the time this  Agreement is filed
with the SEC, of the terms and  conditions  of this  Agreement to anyone but his
immediate family members,  his attorney and/or his other outside  consultants or
professionals who have a need to know the terms and conditions of this Agreement
or as otherwise  required by law shall entitle  Osteotech to recover from Alfaro
all of the  payments  and other  benefits  provided to him under this  Agreement
and/or to seek injunctive relief.  However,  nothing contained in this Agreement
shall be deemed to preclude Alfaro from truthfully  testifying in response to an
order issued by a court of competent  jurisdiction or a validly issued subpoena.
If Alfaro receives a validly issued subpoena or other judicial or quasi-judicial
request  calling  for  the  disclosure  of the  terms  and  conditions  of  this
Agreement,  or any other information relating to Osteotech's business during the
period of his employment by Osteotech, Alfaro shall send copies of the subpoena,
demand or other  request  with any other  accompanying  materials by first class
mail and facsimile to Mr. Richard W. Bauer or his successor at Osteotech. Alfaro
shall  dispatch  such copies  promptly  (i.e.,  no later than two days after his
receipt  of such  subpoena,  demand or  request  and in no event  later than the
return of the subpoena, demand or request).

     12.  Alfaro  agrees that the  benefits  provided by this  Agreement  cannot
continue and will in fact terminate  unless, by no later than February 15, 2002,
(a) a signed copy

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<PAGE>

of this Agreement is delivered to Mr. Richard W. Bauer, or his designee, and (b)
all  Osteotech  property in Alfaro's  possession  is returned to Mr.  Richard W.
Bauer, or his designee.

     13.  Alfaro  acknowledges  and  agrees  that he has  been  given  at  least
twenty-one (21) days to consider this Agreement. Alfaro further acknowledges and
agrees that he may cancel or revoke this  Agreement  within seven (7) days after
signing it. To be effective, any notice of cancellation or revocation must be in
writing and delivered either by hand or mail within such seven (7) day period to
Mr.  Richard  W.  Bauer at  Osteotech.  If  delivered  by mail,  the  notice  of
cancellation  or  revocation  must be (a)  post-marked  within the seven (7) day
period;  (b) properly  addressed to Mr.  Richard W. Bauer,  Osteotech,  Inc., 51
James Way,  Eatontown,  New Jersey 07724; and (c) sent by certified mail, return
receipt  requested.  Alfaro  acknowledges  and agrees that,  if he exercises his
right  of  cancellation  or  revocation,  Osteotech  shall  be  relieved  of all
obligations undertaken in this Agreement.

     14. The terms and conditions of this Agreement may not be altered,  amended
or modified except by a writing duly executed by both Alfaro and Osteotech.

     15. The terms and  conditions of this  Agreement are personal to Alfaro and
Osteotech  and may not be  assigned  by  either  party to any  person  or entity
without the prior written consent of the other party.  However,  in the event of
Alfaro's untimely death prior to November 15, 2002, the balance of such payments
shall be made to his wife, and, in the event of her untimely death,  the balance
of such payments  shall be made to Alfaro's legal heirs.  Neither  Alfaro's wife
nor heirs  shall be  entitled  to receive  any  payments  relating to the period
subsequent to November 15, 2002.

     16. Except as otherwise stated herein,  this Agreement  contains the entire
understanding  between Alfaro and Osteotech  with respect to the  termination of
Alfaro's

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<PAGE>

employment at Osteotech.  There are no covenants,  representations  or
undertakings  with respect to such  termination  other than those  expressly set
forth or referenced in this Agreement.

     17.  If any  portion  of this  Agreement  is found by a court of  competent
jurisdiction to be void and  unenforceable,  such portions shall be deemed to be
severable from the Agreement and shall have no effect on the remaining  sections
of this Agreement.

     18. This Agreement  shall be governed and construed in accordance  with the
laws of the State of New Jersey without regard to its choice of law or conflicts
of law rules.

     19. This  Agreement  has been  reviewed and  negotiated  by both Alfaro and
Osteotech,  and no provision of this Agreement shall be construed against either
party on the ground  that such party was the  drafter of that  provision  or the
Agreement.

     20. This  Agreement  shall be binding  upon Alfaro and  Osteotech  upon its
execution  by them and shall  inure to the  benefit of their  respective  heirs,
successors and permitted assigns.

     IN WITNESS  WHEREOF,  the parties  have  hereunto set their hands as of the
dates set forth below.

OSTEOTECH, INC.

By:  /s/ Richard W. Bauer                           /s/ Arthur A. Alfaro
     --------------------------------           ------------------------------
         Richard W. Bauer, President                    ARTHUR A. ALFARO
         and Chief Executive Officer

Date:          2/18/02                         Date:        2/1/02
     --------------------------------               ---------------------------

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                                    EXHIBIT A

Stock Option Agreement # 1178 for Arthur A. Alfaro

Plan ID:          1991 Plan

Grant Date:       October 1, 1999

Type:             NQ

Price:            $12.6250

Granted:          200,000 options

Vested:           100,000 options

Non-Vested:       100,000 options

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<PAGE>

                                    EXHIBIT B

Stock Option Agreement #1245 for Arthur A. Alfaro

Plan ID:          2000 Plan

Grant Date:       December 7, 2000

Type:             ISO

Price:            $3.5000

Granted:          100,000 options

Vested:           25,000 options

Non-Vested:       75,000 options

                                                                           E-169

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