董培新

董 培新（ドン バイシン; Dong Peixin）は、日本の癌研究者、医学博士。北海道大学医学部。 1997年6月 中国ハルビン医科大学卒業 1997年8月 中国ハルビン医科大学付属黒龍江省電力病院婦人科医員 2002年10月 福岡大学医学部産婦人科研究員 2004年4月 北海道大学大学院医学研究科婦人科専攻入学 2008年3月 北海道大学大学院医学研究科婦人科専攻終了(博士号取得) 2008年4月 中国上海復旦大学付属病院産婦人科病院助手 2009年4月 中国上海復旦大学付属病院産婦人科病院講師 2010年4月 北海道大学医学研究科産婦人科学分野 2011年度 北海道産科婦人科学会賞（October 28, 2012）受賞 2015年度 北海道大学研究総長賞（February 3, 2016）受賞 日本産婦人科学会（会員：2011年～現在） 北海道産婦人科学会（会員：2011年～現在） 日本RNA学会（会員：2014年～現在） 国際誌Editorial Board Member： Journal of Cancer Research & Therapy、2013年～2015年 Cancer Growth and Metastasis、2013年～2015年 Austin Journal of Women’s Health、2014年～現在 Women's Health Research、2017～現在 国際誌reviewer： Oncotarget Scientific Reports Journal of Translational Medicine Gynecologic Oncology BMC Cancer Journal of Experimental & Clinical Cancer Research FEBS Letters Molecular and cellular biochemistry International Journal of Gynecology & Obstetrics 癌転移 婦人科癌; endometrial carcinoma; cervical cancer; ovarian cancer microRNA EMT: epithelial to mesenchymal transition (EMT) Twist1 gene, a critical EMT inducer in endometrial cancer en:Cervical_cancer cervical cancer p53: TP53, tumor suppressor in human tumors endometrial cancer ovarian cancer miR-194 is a tumor suppressor in endometrial cancer Prognostic significance of miR-194 in endometrial cancer miR-130b in endometrial cancer miR-101 is key tumor suppressor in both type I and type II endometrial cancer IQGAP1 is novel oncogene in endometrial cancer and ovarian cancer miR-106b acts as tumor suppressor in aggressive in endometrial cancer KLF17 gene induces EMT in endometrial cancer miR-31 functions as an oncogene by suppressing Hippo tumor suppressor pathway in endometrial cancer MiR-137 and miR-34a directly target Snail and inhibit EMT, invasion and sphere-forming ability of ovarian cancer cells. 35, Article number: 132 (2016) iASPP and p53 in human tumors miRNA-mediated PI3K/AKT signaling in endometrial cancer miRNA-124 directly targets and inhibits IQGAP1 expression in endometrial cancer EZH2 induces Twist expression by repressing miR-361 expression in aggressive type II endometrial cancer CRISPR/Cas9 vector mediated miR-21 gene editing inhibits the epithelial to mesenchymal transition in ovarian cancer cells lentiviral CRISPR/Cas9-mediated disruption of miR-21 inhibits EMT in ovarian cancer cells miR-124 binds to and suppresses oncogene iASPP expression in cervical cancer iASPP induces EMT and cisplatin resistance through miR-20a-FBXL5/BTG3 signaling in cervical cancer p53 gain of function mutations promote endometrial cancer progression and metastasis EZH2, MCL-1 and FOS gene are important oncogenes and EMT inducers in endometrial cancer CRISPR/Cas9 and and genome editing ZEB1 acts as a novel EMT inducer in endometrial cancer BMI-1 gene functions as a important EMT inducer in endometrial cancer cells Musashi-2 (MSI-2) has a crucial role in promoting cervical cancer metastasis PD-L1 is a critical oncogenic factor in cervical cancer progression, and miR-140/142/340/383 regulate the expression of PD-L1 in cervical cancer Exploring lncRNA-Mediated Regulatory Networks in Endometrial Cancer Long noncoding RNA NEAT1 drives aggressive endometrial cancer progression via miR-361-regulated networks involving STAT3 and tumor microenvironment-related genes B7H3 As a Promoter of Metastasis and Promising Therapeutic Target miR-203 inhibits ovarian tumor metastasis by targeting BIRC5 and attenuating the TGFβ pathway KLF4 expression enhances the efficacy of chemotherapy drugs in ovarian cancer cells Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells miR-34a, miR-424 and miR-513 inhibit MMSET expression to repress endometrial cancer cell invasion and sphere formation Molecular-targeted therapies and precision medicine for endometrial cancer Lentiviral CRISPR/Cas9 nickase vector mediated BIRC5 editing inhibits epithelial to mesenchymal transition in ovarian cancer cells The Expression, Functions and Mechanisms of Circular RNAs in Gynecological Cancers Ovarian Primary and Metastatic Tumors Suppressed by Survivin Knockout or a Novel Survivin Inhibitor Yue, Junming and Du, Ziyun and Zhou, Fu-Ming and Dong, Peixin and Pfeffer, Lawrence M「Applications of CRSIPR/Cas9 in Cancer Research」『Cancer Medicine & Anti Cancer Drugs』第1巻第1号、OMICS International、2016年3月、1000103頁、NAID 120005752854。  LncRNA NEAT1-mediated miR-361 downregulation contributes to EMT of cervical cancer cells via increasing HSP90 expression: Epithelial-mesenchymal transition (EMT) is a key process contributing to cervical cancer (CC) metastasis, and microRNAs (miRNAs) modulate the expression of genes implicated in EMT. However, the accurate role of miR-361 in CC-associated EMT and the mechanisms underlying its function in CC remains largely unknown. The functional roles of miR-361 in CC cells were explored by a series of cell functional assays. Luciferase reporter assays were used to demonstrate the potential interaction between miR-361, HSP90, and long non-coding RNA (lncRNA) NEAT1. We detected a reduction of miR-361 expression in CC tissues compared with normal tissues, and miR-361 overexpression inhibited invasion and EMT phenotypes of CC cells by directly targeting a key EMT activator HSP90. Additionally, we detected significantly higher levels of HSP90 in CC tissues compared with normal tissues, and high expression of HSP90 predicted a poorer prognosis. We further identified NEAT1 as a significantly upregulated lncRNA in CC tissues and high expression of NEAT1 was associated with worse survival in CC patients. NEAT1 directly repressed miR-361 expression and played an oncogenic role in CC cell invasion and sphere formation. Conclusions: These results demonstrated that miR-361 directly targets HSP90 to inhibit the invasion and EMT features, and NEAT1 functions as an oncogenic lncRNA that suppresses miR-361 expression and induces EMT and sphere formation in CC cells, thus providing critical insights into the molecular pathways operating in this malignancy. MicroRNA-361: A Multifaceted Player Regulating Tumor Aggressiveness and Tumor Microenvironment Formation PD-L1 Is a Tumor Suppressor in Aggressive Endometrial Cancer Cells and Its Expression Is Regulated by miR-216a and lncRNA MEG3 Background: Poorly differentiated endometrioid adenocarcinoma and serous adenocarcinoma represent an aggressive subtype of endometrial cancer (EC). Programmed death-ligand-1 (PD-L1) was known to exhibit a tumor cell-intrinsic function in mediating immune-independent tumor progression. However, the functional relevance of tumor cell-intrinsic PD-L1 expression in aggressive EC cells and the mechanisms regulating its expression remain unknown. Methods: PD-L1 expression in 65 EC tissues and 18 normal endometrium samples was analyzed using immunohistochemical staining. The effects of PD-L1 on aggressive EC cell growth, migration and invasion were investigated by cell functional assays. Luciferase reporter assays were used to reveal the microRNA-216a (miR-216a)-dependent mechanism modulating the expression of PD-L1. Results: Positive PD-L1 expression was identified in 84% of benign cases but only in 12% of the EC samples, and the staining levels of PD-L1 in EC tissues were significantly lower than those in the normal tissues. Higher PD-L1 expression predicts favorable survival in EC. Ectopic expression of PD-L1 in aggressive EC cells results in decreased cell proliferation and the loss of mesenchymal phenotypes. Mechanistically, PD-L1 exerts the anti-tumor effects by downregulating MCL-1 expression. We found that PD-L1 levels in aggressive EC cells are regulated by miR-216a, which directly targets PD-L1. We further identified a mechanism whereby the long non-coding RNA MEG3 represses the expression of miR-216a, thereby leading to increased PD-L1 expression and significant inhibition of cell migration and invasion. Conclusion: These results reveal an unappreciated tumor cell-intrinsic role for PD-L1 as a tumor suppressor in aggressive EC cells, and identify MEG3 and miR-216a as upstream regulators of PD-L1. microRNAs are novel prognostic biomarkers and EMT modulators, and act as either oncogeic miRNAs (OncomiRs) or tumor suppressors in endometrial cancer. Mutant p53 Gain-of-Function in Cancer: Besides the dominant negative effects upon wild type p53, increasing evidence suggests that p53 mutations acquire additional oncogenic functions, such as a gain-of-function (GOF), which actively drive cells toward invasion and metastasis7 through transactivation or transrepression of a large set of genes involved in regulation of cell adhesion, migration, and proliferation. Restoration of tumor suppressor miRNAs: The loss or down-regulation of a tumor suppressor miRNA could be overcome by the restoration of these silenced miRNAs. Blocking oncogenic miRNAs: Another strategy to target miRNA expression in cancer is to block the expression of an oncogenic miRNA. CRISPR/Cas9-Mediated Genome Editing of Epigenetic Factors for Cancer Therapy. Interconnection between EMT and stemness in gynecological cancers. miRNAs regulates endometrial cancer metastasis through modulating tumor microenvironment. eBook: Peixin Dong: Molecular targets and targeted therapy. pp. 182-199. (Noriaki Sakuragi eds., Current Approaches to Endometrial Cancer. Future Medicine Ltd, UK), 2014. Peixin Dong*, Masanori Kaneuchi, Yosuke Konno, Hidemichi Watari, Satoko Sudo, Noriaki Sakuragi: Emerging therapeutic biomarkers in endometrial cancer. Biomed Res Int 2013, 130362-130373, 2013 (*Corresponding author) Peixin Dong*, Yosuke Konno, Hidemichi Watari, Masayoshi Hosaka, Masayuki Noguchi, Noriaki Sakuragi: The impact of microRNA-mediated PI3K/AKT signaling on epithelial-mesenchymal transition and cancer stemness in endometrial cancer. J Transl Med 12, 231-240, 2014 Peixin Dong*, Kei Ihira, Junichi Hamada, Hidemichi Watari, Takahiro Yamada, Masayoshi Hosaka, Sharon J.B. Hanle, Masataka Kudo and Noriaki Sakuragi: Reactivating p53 functions by suppressing its novel inhibitor iASPP: a potential therapeutic opportunity in p53 wild-type tumors. Oncotarget 6, 19968-19975, 2015 Dong P, Nabeshima K*, Nishimura N, Kawakami T, Hachisuga T, Kawarabayashi T, Iwasaki H: Overexpression and diffuse expression pattern of IQGAP1 at invasion fronts are independent prognostic parameters in ovarian carcinomas. Cancer Lett 243, 120-127, 2006 (IF=4.960) Dong P, Tada M, Hamada J, Nakamura A, Moriuchi T, Sakuragi N*: p53 dominant-negative mutation R273H promotes invasion and migration of human endometrial cancer HHUA cells. Clin Exp Metastasis 24, 471-483, 2007 (IF=3.770) Dong P*, Jia N, Xu ZJ, Liu YT, Li DJ, Feng YJ: Silencing of IQGAP1 by shRNA inhibits the invasion of ovarian carcinoma HO-8910PM cells in vitro. J Exp Clin Cancer Res 27, 77-85, 2008 (IF=3.730) Jia N*, Dong P, Huang Q, Jin W, Zhang J, Dai Q, Liu S: Cardioprotective effects of granulocyte colony-stimulating factor in Angiotensin-II Induced Cardiac Remodeling. Clin Exp Pharmacol Physiol 36, 262-266, 2009 (IF=2.370) Dong P*, Xu Z, Jia N, Li D, Feng Y: Elevated expression of p53 gain-of-function mutation R175H in endometrial cancer cells can increase the invasive phenotypes by activation of the EGFR/PI3K/AKT pathway. Mol Cancer 8, 103-110, 2009 (IF=5.220) Peixin Dong, Masanori Kaneuchi, Hidemichi Watari, Junichi Hamada, Satoko Sudo, Jingfang Ju, Noriaki Sakuragi*: miR-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1. Mol Cancer 10, 99-108, 2011 (IF=5.220) Nan Jia*, Peixin Dong, Ying Ye, Cheng Qian, Qiuyan Dai: Allopurinol attenuates oxidative stress and cardiac fibrosis in angiotensin II-induced cardiac diastolic dysfunction. Cardiovasc Ther 30, 117-123, 2012 (IF=2.444) Tatsuya Kato, Hidemichi Watari*, Daisuke Endo, Takashi Mitamura, Tetsuji Odagiri, Yousuke Konno, Masayoshi Hosaka, Noriko Kobayashi, Yukiharu Todo, Satoko Sudo, Mahito Takeda, Peixin Dong, Masanori Kaneuchi, Masataka Kudo, Noriaki Sakuragi: New revised FIGO 2008 staging system for endometrial cancer produces better discrimination in survival compared with the 1988 staging system. J Surg Oncol 106, 938-941, 2012 (IF=3.088) P Dong*, M Karaayvaz, N Jia, M Kaneuchi, J Hamada, H Watari, S Sudo, J Ju, N Sakuragi: Mutant p53 gain-of-function induces epithelial–mesenchymal transition through modulation of the miR-130b–ZEB1 axis. Oncogene 32, 3286-3295, 2013 (IF=7.632) Haiyan Zhai, Mihriban Karaayvaz, Peixin Dong, Noriaki Sakuragi, Jingfang Ju*: Prognostic significance of miR-194 in endometrial cancer. Biomark Res 1, 12-17, 2013 (IF=0.000) Peixin Dong*, Masanori Kaneuchi, Ying Xiong, Liping Cao, Muyan Cai, Xishi Liu, Sun-Wei Guo, Jingfang Ju, Nan Jia, Yosuke Konno, Hidemichi Watari, Masayoshi Hosaka, Satoko Sudo, Noriaki Sakuragi: Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer. Carcinogenesis 35, 760-768, 2014 (IF=5.698) Tetsuji Odagiri, Hidemichi Watari*, Tatsuya Kato, Takashi Mitamura, Masayoshi Hosaka, Satoko Sudo, Mahito Takeda, Noriko Kobayashi, Peixin Dong, Yukiharu Todo, Masataka Kudo, Noriaki Sakuragi: Distribution of Lymph Node Metastasis Sites in Endometrial Cancer Undergoing Systematic Pelvic and Para-Aortic Lymphadenectomy: A Proposal of Optimal Lymphadenectomy for Future Clinical Trials. Ann Surg Oncol 21, 2755-2761, 2014 (IF=4.530) Peixin Dong*, Masanori Kaneuchi, Hidemichi Watari, Satoko Sudo, Noriaki Sakuragi: miR-106b modulates epithelial-mesenchymal transition by targeting TWIST1 in invasive endometrial cancer cell lines. Molecular Carcinog 53, 349-359, 2014 (IF=4.180) Yosuke Konno, Peixin Dong*, Ying Xiong, Fumihiko Suzuki, Jiabin Lu, Muyan Cai, Hidemichi Watari, Takashi Mitamura, Masayoshi Hosaka, Sharon J B Hanley, Masataka Kudo, Noriaki Sakuragi: MicroRNA-101 targets EZH2, MCL-1 and FOS to suppress proliferation, invasion and stem cell-like phenotype of aggressive endometrial cancer cells. Oncotarget 5, 6049-6062, 2014 (IF=6.402) Takashi Mitamura, Hidemichi Watari*, Lei Wang, Hiromi Kanno, Masaya Miyazaki, Makiko Kitagawa, Mohamed Kamel Hassan, Peixin Dong, Taichi Kimura, Mishie Tanino, Hiroshi Nishihara, Shinya Tanaka and Noriaki Sakuragi: miR-31 functions as an endometrial cancer oncogene by suppressing Hippo tumor suppressor pathway. Mol Cancer 13, 97-108, 2014 (IF=5.220) Hanley SJ*, Fujita H, Yokoyama S, Kunisawa S, Tamakoshi A, Dong P, Kobayashi N, Watari H, Kudo M, Sakuragi N: HPV self-sampling in Japanese women: A feasibility study in a population with limited experience of tampon use. J Med Screen. 23, 164-70, 2016 (5-year IF =2.478) Dong P*, Ihira K, Xiong Y, Watari H, Hanley SJ, Yamada T, Hosaka M, Kudo M, Yue J, Sakuragi N: Reactivation of epigenetically silenced miR-124 reverses the epithelial-to-mesenchymal transition and inhibits invasion in endometrial cancer cells via the direct repression of IQGAP1 expression. Oncotarget. 12, 20260-70, 2016 (5-year IF=5.312) Dong P*, Xiong Y, Watari H, Hanley SJ, Ihira K, Konno Y, Yamada T, Hosaka M, Kudo M, Yue J, Sakuragi N: miR-137 and miR-34a directly target Snail and inhibit EMT, invasion and sphere-forming ability of ovarian cancer cells. Journal of Experimental & Clinical Cancer Research 35, 132-141, 2016 (5-year IF=4.590) Dong P*, Xiong Y, Watari H, Hanley SJ, Konno Y, Ihira K, Suzuki F, Yamada T, Kudo M, Yue J, Sakuragi N: Suppression of iASPP-dependent aggressiveness in cervical cancer through reversal of methylation silencing of miR-124. Sci Rep. 6, 35480-35491, 2016 (5-year IF=4.874) Huo W, Zhao G, Yin J, Xuan O, Wang Y, Yang C, Wang B, Dong P* (co-corresponding author), Wang Z, Watari H, Chaum E, Pfeffer L, Yue J: Lentiviral CRISPR/Cas9 vector mediated miR-21 knockout inhibits the epithelial to mesenchymal transition in ovarian cancer cells. Journal of Cancer, 8, 57-64, 2017 (5-year IF=3.087) Wang B, Shen A, Ouyang X, Zhao G, Du Z, Huo W, Zhang T, Wang Y, Yang C, Dong P* (co-corresponding author), Watari H, Pfeffer LM, Yue J: KLF4 expression enhances the efficacy of chemotherapy drugs in ovarian cancer cells. Biochem Biophys Res Commun. 484, 486-492, 2017 (5-year IF=2.354) Ihira K, Dong P* (co-first author), Xiong Y, Watari H, Konno Y, Hanley SJ, Noguchi M, Hirata N, Suizu F, Yamada T, Kudo M, Sakuragi N: EZH2 inhibition suppresses endometrial cancer progression via miR-361/Twist axis. Oncotarget. 8, 13509-13520, 2017 (5-year IF=5.312) Xiong Y, Sun F, Dong P* (co-corresponding author), Watari H, Yue J, Yu M, Lan C, Wang Y, Ma Z: iASPP induces EMT and cisplatin resistance in human cervical cancer through miR-20a-FBXL5/BTG3 signaling. Journal of Experimental & Clinical Cancer Research 36, 48-58, 2017 (5-year IF=4.590) Zhang Q, Dong P* (co-first author), Liu X, Sakuragi N, Guo SW. Enhancer of Zeste homolog 2 (EZH2) induces epithelial-mesenchymal transition in endometriosis. Sci Rep. 7, 6804-6816, 2017 (5-year IF=4.874) Zhao G, Wang Q, Gu Q, Dong P* (co-corresponding author), Watari H, Li W, Yue J. Lentiviral CRISPR/Cas9 nickase vector mediated BIRC5 editing inhibits epithelial to mesenchymal transition in ovarian cancer cells. Oncotarget, accepted, 2017 (5-year IF=5.312) Dong P*, Xiong Y, Hanley SJ, Yue J, Watari H. Musashi-2, a novel oncoprotein promoting cervical cancer cell growth and invasion, is negatively regulated by p53-induced miR-143 and miR-107 activation. Journal of Experimental & Clinical Cancer Research, accepted, 2017 (5-year IF=4.590) Dong P*, Yue J. Lentiviral vector mediated-ASAP1 expression promotes epithelial to mesenchymal transition in ovarian cancer cells. ONCOLOGY LETTERS, accepted, 2018 董 培新 Dong Peixin