CD34

CD34（小鼠、大鼠的同源蛋白寫作Cd34），是一類分化簇（Cluster of differentiation, CD）分子，在人體內由CD34基因編碼，主要用來鑑定造血幹細胞（HSC）等成體幹細胞。CD34化學本質爲糖蛋白，正常情況下位於細胞膜表面，功能包括參與細胞—細胞間黏附和細胞—細胞外基質（ECM）黏附。 CD34最早於20世紀80年代由兩個課題組同時獨立發現，最初是用來鑑定一類造血幹細胞（CD34+陽性）。现在，是否表達CD34也是分離、鑑別造血幹細胞及其亚群（subpopulation）的一項重要指標。CD34为单跨膜的黏液素（Mucin）類糖蛋白，通常分佈於細胞膜表面。CD34功能複雜，至今CD34的功能仍然未完全闡明，已知的CD34分子功能主要是參與細胞黏附。 CD34蛋白主要表達於造血幹細胞和造血祖細胞中，同時，血管內皮細胞、一部分間充質幹細胞（MSC）也表達CD34。CD34在臍帶和骨髓中表達強度相對較高。 分化簇（CD） CD44 GRCh38: Ensembl release 89: ENSG00000174059 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000016494 - Ensembl, May 2017 Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.  Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.  Entrez Gene: CD34 CD34 molecule. （原始内容存档于2019-09-24）.  Simmons DL, Satterthwaite AB, Tenen DG, Seed B. Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells. Journal of Immunology. Jan 1992, 148 (1): 267–71 [2018-02-14]. PMID 1370171. （原始内容存档于2008-06-06）.  Satterthwaite AB, Burn TC, Le Beau MM, Tenen DG. Structure of the gene encoding CD34, a human hematopoietic stem cell antigen. Genomics. Apr 1992, 12 (4): 788–94. PMID 1374051. doi:10.1016/0888-7543(92)90310-O.  Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. Journal of Immunology. 1984, 133 (1): 157–65. PMID 6586833.  Tindle RW. Nichols R. Chan L. Campana D. Birnie GD. A novel monoclonal antibody BI-3C5 recognises myeloblasts and non-B, non-T lymphoblasts in acute leukaemia and CGL blast crises, and react with immature cells in normal bone marrow. Leukaemia Research. 1985, 9: 1–9. doi:10.1016/0145-2126(85)90016-5.  Tindle RW. Katz F. Martin H. Watt D. Catovsky D. Janossy G. Greaves M. BI-3C5 (CD34) defines multipotential and lineage restricted progenitor cells and their leukaemic counterparts .. In 'Leucocyte typing 111: White cell differentiation antigens. Oxford University Press, 654-655. 1987.  Loken M. Shah V. Civin CI.. Characterization of myeloid antigens on human bone marrow using multicolour immunofluorescence. In: McMichael, Leucocyte Typing III:White cell differentiation antigens.Oxford University Press 630-635. 1987.  Furness SG, McNagny K. Beyond mere markers: functions for CD34 family of sialomucins in hematopoiesis. Immunologic Research. 2006, 34 (1): 13–32. PMID 16720896. doi:10.1385/IR:34:1:13.  Srivastava A, Bapat M, Ranade S, Srinivasan V, Murugan P, Manjunath S, Thamaraikannan P, Abraham S. Autologous Multiple Injections of in Vitro Expanded Autologous Bone Marrow Stem Cells For Cervical Level Spinal Cord Injury - A Case Report. Journal of Stem Cells and Regenerative Medicine. 2010 [2018-02-14]. （原始内容存档于2018-04-10）.  Nielsen JS, McNagny KM. Novel functions of the CD34 family. Journal of Cell Science. Nov 2008, 121 (Pt 22): 3683–92. PMID 18987355. doi:10.1242/jcs.037507.  Drew E, Merzaban JS, Seo W, Ziltener HJ, McNagny KM. CD34 and CD43 inhibit mast cell adhesion and are required for optimal mast cell reconstitution. Immunity. Jan 2005, 22 (1): 43–57. PMID 15664158. doi:10.1016/j.immuni.2004.11.014.  Strilić B, Kucera T, Eglinger J, Hughes MR, McNagny KM, Tsukita S, Dejana E, Ferrara N, Lammert E. The molecular basis of vascular lumen formation in the developing mouse aorta. Developmental Cell. Oct 2009, 17 (4): 505–15. PMID 19853564. doi:10.1016/j.devcel.2009.08.011.  Ogawa M, Tajima F, Ito T, Sato T, Laver JH, Deguchi T. CD34 expression by murine hematopoietic stem cells. Developmental changes and kinetic alterations. Annals of the New York Academy of Sciences. Jun 2001, 938: 139–45. Bibcode:2001NYASA.938..139O. PMID 11458501. doi:10.1111/j.1749-6632.2001.tb03583.x.  Sidney LE, Branch MJ, Dunphy SE, Dua HS, Hopkinson A. Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem Cells. Jun 2014, 32 (6): 1380–9. PMC 4260088 . PMID 24497003. doi:10.1002/stem.1661.