Muse細胞

Muse細胞（Multi-lineage differentiating stress enduring cell，多向分化壓力耐受細胞）是由日本東北大學出澤真理課題組於2010年發現一種內源性、無成瘤性的多能幹細胞。幾乎所有不同器官來源的結締組織（如骨髓、外周血）中都有Muse細胞。通常Muse細胞可從商業化的間充質細胞中分離到，如人成纖維細胞、骨髓間充質幹細胞，以及脂肪源幹細胞（adipose-derived stem cells）。Muse細胞可自發分化或在細胞因子誘導作用下分化爲三個胚層的細胞。Muse細胞的成瘤性較低，注入新的宿主體內也不會形成腫瘤，目前的觀點認爲這是因爲該細胞的端粒酶活性較低。Muse細胞同時被應用於小鼠骨骼肌退變、糖尿病性皮膚潰瘍及急性重症肝炎等模型的修復研究中，研究發現Muse細胞在損傷組織中能夠和宿主的組織順利整合，並且分別向相應胚層的細胞分化。有研究利用Muse細胞修復腦動脈堵塞後，引起局部缺血的中風小鼠模型，發現Muse細胞修現可以替代丟失的神經元，整合至梗死的皮質組織中，分化為神經元標誌物表達陽性的細胞，並且有較為明顯的功能修復。 當Muse細胞被放置到含有營養因子和細胞因子的培養基中進行培養時，超過百分之九十的細胞都會進行定向細胞分化。若該培養基含有肝細胞生長因子、成纖維細胞生長因子4和地塞米松的胰島素-轉鐵蛋白-亞硒酸鈉時，百分之九十以上的細胞會分化成肝細胞，並且在α-胎兒蛋白和人類白蛋白表達中呈現陽性反應。在骨或脂肪細胞的培養基中，百分之九十八以上的細胞會分化為骨鈣蛋白或油紅O陽性的細胞。 胚胎幹細胞 間充質 iPS細胞 Kuroda, Y.; Kitada, M.; Wakao, S.; Nishikawa, K.; Tanimura, Y.; Makinoshima, H.; Goda, M.; Akashi, H.; Inutsuka, A.; Niwa, A.; Shigemoto, T.; Nabeshima, Y.; Nakahata, T.; Nabeshima, Y.-i.; Fujiyoshi, Y.; Dezawa, M. Unique multipotent cells in adult human mesenchymal cell populations. Proceedings of the National Academy of Sciences. 2010, 107 (19): 8639–43. Bibcode:2010PNAS..107.8639K. PMC 2889306 . PMID 20421459. doi:10.1073/pnas.0911647107.  Wakao, S.; Kitada, M.; Kuroda, Y.; Shigemoto, T.; Matsuse, D.; Akashi, H.; Tanimura, Y.; Tsuchiyama, K.; Kikuchi, T.; Goda, M.; Nakahata, T.; Fujiyoshi, Y.; Dezawa, M. Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proceedings of the National Academy of Sciences. 2011, 108 (24): 9875–80. Bibcode:2011PNAS..108.9875W. PMC 3116385 . PMID 21628574. doi:10.1073/pnas.1100816108.  Dezawa, Mari. Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells: Direct Contribution of Muse Cells to Tissue Regeneration. Cell Transplantation. 2016, 25 (5): 849–61. PMID 26884346. doi:10.3727/096368916X690881.  Hori, Emiko; Hayakawa, Yumiko; Hayashi, Tomohide; Hori, Satoshi; Okamoto, Soushi; Shibata, Takashi; Kubo, Michiya; Horie, Yukio; Sasahara, Masakiyo; Kuroda, Satoshi. Mobilization of Pluripotent Multilineage-Differentiating Stress-Enduring Cells in Ischemic Stroke. Journal of Stroke and Cerebrovascular Diseases. 2016, 25 (6): 1473–81. PMID 27019988. doi:10.1016/j.jstrokecerebrovasdis.2015.12.033.  Kuroda, Yasumasa; Wakao, Shohei; Kitada, Masaaki; Murakami, Toru; Nojima, Makoto; Dezawa, Mari. Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells. Nature Protocols. 2013, 8 (7): 1391–415. PMID 23787896. doi:10.1038/nprot.2013.076.  Heneidi, Saleh; Simerman, Ariel A.; Keller, Erica; Singh, Prapti; Li, Xinmin; Dumesic, Daniel A.; Chazenbalk, Gregorio. Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue. PLoS ONE. 2013, 8 (6): e64752. Bibcode:2013PLoSO...864752H. PMC 3673968 . PMID 23755141. doi:10.1371/journal.pone.0064752.  Simerman, AA; Dumesic, DA; Chazenbalk, GD. Pluripotent muse cells derived from human adipose tissue: a new perspective on regenerative medicine and cell therapy.. Clinical and translational medicine. 2014, 3: 12 [2019-11-22]. PMID 24940477. doi:10.1186/2001-1326-3-12.  Wakao, S; Akashi, H; Kushida, Y; Dezawa, M. Muse cells, newly found non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues.. Pathology international. 2014-01, 64 (1): 1–9 [2019-11-22]. PMID 24471964. doi:10.1111/pin.12129.  Kuroda, Y; Dezawa, M. Mesenchymal stem cells and their subpopulation, pluripotent muse cells, in basic research and regenerative medicine.. Anatomical record (Hoboken, N.J. : 2007). 2014-01, 297 (1): 98–110 [2019-11-22]. PMID 24293378. doi:10.1002/ar.22798.  Yamauchi, T; Kuroda, Y; Morita, T; Shichinohe, H; Houkin, K; Dezawa, M; Kuroda, S. Therapeutic effects of human multilineage-differentiating stress enduring (MUSE) cell transplantation into infarct brain of mice.. PloS one. 2015, 10 (3): e0116009 [2019-11-22]. PMID 25747577. doi:10.1371/journal.pone.0116009.  Ogura, Fumitaka; Wakao, Shohei; Kuroda, Yasumasa; Tsuchiyama, Kenichiro; Bagheri, Mozhdeh; Heneidi, Saleh; Chazenbalk, Gregorio; Aiba, Setsuya; Dezawa, Mari. Human Adipose Tissue Possesses a Unique Population of Pluripotent Stem Cells with Nontumorigenic and Low Telomerase Activities: Potential Implications in Regenerative Medicine. Stem Cells and Development. 2014, 23 (7): 717–28. PMID 24256547. doi:10.1089/scd.2013.0473.