Caco-2細胞

Caco-2是人類結腸腺癌細胞系，最初是在1977年由紀念斯隆-凱特琳癌症中心的研究員從一名72歲的白人男性結腸腺癌患者分離出來。 Caco-2細胞擁有與小腸上皮細胞刷狀緣（英语：Brush border）相關的酶系（英语：Cyclic enzyme system），而且其結構、標誌酶的特異性表達與滲透性等方面均與人類小腸上皮細胞相近。在體外培養Caco-2細胞的過程中，會發現生長在多孔膜上的Caco-2細胞會形成朝向上皮樣分化的單細胞層（英语：Monolayer）。Caco-2細胞具有微絨毛結構，並且與相鄰的細胞緊密地結合，同時又會分泌着水解酶等酶，以及合成氨基酸等的載體轉運系統。 Caco-2細胞在遺傳學等方面均是最令人滿意的上皮細胞系，所以Caco-2細胞構建的模型，不僅是吸收營養素及毒素效果最良好的體外模型，還可以模擬小腸上皮細胞吸收、代謝及轉運口服藥物與營養物質分子等的過程。這可以預測不同轉運途徑的食物營養物質在體內的吸收、營養物質的化學結構和體內轉運關係、營養物質代謝穩定性及氫離子濃度指數對營養物質吸收的影響等。因此，Caco-2細胞廣泛應用於開發新型藥物、研究腸內藥物吸收（英语：Absorption (pharmacology)）機制等，甚至被應用在葉酸吸收與紅血球生成素作用的研究。 C2BBe1细胞 C2BBe2细胞 Caco-2/3细胞 Hidalgo, IJ; Raub, TJ; Borchardt, RT. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability.. Gastroenterology. 1989-03, 96 (3): 736–49 [2019-12-17]. PMID 2914637. （原始内容存档于2019-12-17）.  Hubatsch, I; Ragnarsson, EG; Artursson, P. Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers.. 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Effect of cooking, digestion and Caco-2 cells absorption on the radical scavenging activities of edible mushrooms. International Journal of Food Science & Technology. 2009-11, 44 (11): 2189–2197. doi:10.1111/j.1365-2621.2009.02059.x.  使用|accessdate=需要含有|url= (帮助) Ranaldi, G; Marigliano, I; Vespignani, I; Perozzi, G; Sambuy, Y. The effect of chitosan and other polycations on tight junction permeability in the human intestinal Caco-2 cell line(1).. The Journal of nutritional biochemistry. 2002-03, 13 (3): 157–167 [2019-12-22]. PMID 11893480. doi:10.1016/s0955-2863(01)00208-x. （原始内容存档于2019-12-22）.  Yi, W; Akoh, CC; Fischer, J; Krewer, G. Absorption of anthocyanins from blueberry extracts by caco-2 human intestinal cell monolayers.. Journal of agricultural and food chemistry. 2006-07-26, 54 (15): 5651–8 [2019-12-22]. PMID 16848559. doi:10.1021/jf0531959. （原始内容存档于2019-12-22）.  Johnston, K; Sharp, P; Clifford, M; Morgan, L. 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Unique uptake and transport of isoflavone aglycones by human intestinal caco-2 cells: comparison of isoflavonoids and flavonoids.. The Journal of nutrition. 2002-07, 132 (7): 1956–61 [2019-12-23]. PMID 12097676. doi:10.1093/jn/132.7.1956. （原始内容存档于2019-12-23）.  Akao, T; Hanada, M; Sakashita, Y; Sato, K; Morita, M; Imanaka, T. Efflux of baicalin, a flavone glucuronide of Scutellariae Radix, on Caco-2 cells through multidrug resistance-associated protein 2.. The Journal of pharmacy and pharmacology. 2007-01, 59 (1): 87–93 [2019-12-23]. PMID 17227625. doi:10.1211/jpp.59.1.0012. （原始内容存档于2019-12-23）.  Cellosaurus entry for Caco-2（页面存档备份，存于互联网档案馆）