Bioinformatics python capstone lesson

This lesson is intended to be a 1.5 hour demo that will sit at the end of a novice python bootcamp and show learners how to apply some of their new skills to bioinformatics. Currently work in progress, contributions/suggestions very welcome!
I like the figure - but find it a bit confusing! Some of those intersections are clearly unions! and one is the complement! Maybe crop the image to the simplest case?