Bioinformatics python capstone lesson

This lesson is intended to be a 1.5 hour demo that will sit at the end of a novice python bootcamp and show learners how to apply some of their new skills to bioinformatics. Currently work in progress, contributions/suggestions very welcome!
What about leading with something a more general intro? Or I maybe anyone in this lesson already knows why they would want to do these things? i.e. "A common task for biologists is taking raw sequencing reads, aligning them to a reference genome, and calling variants among individuals. Although there are several software options to perform these tasks, today we will walk you through using bedtools to ... (I haven't read the whole thing yet so I don't actually know :)"