This is a workable CurveAlign Beta version 3.0 primarily based on Jeremy Bredfeldt's updates. Compared to the version 2.3, the major features are : (1) output up to 34 fiber features, including angle, alignment, density, and etc. , these features are saved both in .cvs file and .mat file, which can then be used to potentially classify images or fibers using machine learning techniques; (2)tiff boundary can loaded to investigate the interaction between fiber and cell boundary; (3)CT-FIRE fiber extraction results can be loaded; (4)a matlab svm feature ranking is included; and (5) keep the most features of the older version (2.3), such as the statistics for the relative angles, heat map to show the fiber(angle) alignment, and the boundary association, etc.