Paper: pyFRET: A Python Library for Single Molecule Fluorescence Data Analysis (R. Murphy, S. Jackson, D. Klenerman)

Paper for EuroScipy 2014 proceedings :)
Judging from the examples you show, these distributions don't look much like Gaussians. In particular they are very asymmetric. I realize that you are only trying to implement accepted techniques in your field, so perhaps I should complain somewhere else, but if you can offer any explanation of why people fit Gaussians, this would be nice.