update pod
update pod
update design targets script to deal with mouse genes, also output 2 files, one with targets one with design parameters
rename design targets file, no longer just human targets
update pod
add option to look at only one exon
skip comment columns in parameter input csv, also remove colon from MGI accession ids when creating folders
print out Ensembl db details
initial commit of primer3 util module
primer3 util module now sets up primer3 with global arguments and can be called multiple time with specific sequence and target criteria
initial commit of find-gibson-oligos command
add new method to get slice instead of raw sequence
parse primer information from primer3, store in hashes and produce primer and primer pair yaml files
change name of aos_output folder to the more generic oligo_finder_output, use this folder when finding gibson oligos through primer3
more debug output for non matching sequence, code tidy up
add gibson design method and oligo names
add oligo classes for gibson designs to consolidate command
add note about more logging
add note about refactoring that is needed
fix parsing of primer3 oligo data, we need to revcomp some sequences and work out genome coordiantes depending on if target is on +ve or -ve strand