Patent ID: 10131941

Abstract:
An application of thiolated single-stranded DNA enhances specific amplification of polymerase chain reaction, namely a method for enhancing specific amplification of polymerase chain reaction by utilizing thiolated single-stranded DNA, which includes the following step: adding an appropriate amount of the thiolated single-stranded DNA into a PCR system to perform PCR amplification, wherein the appropriate amount means that the final concentration of the thiolated single-stranded DNA in a 20 μL reaction system is not less than 15 μM. The thiolated single-stranded DNA meets the following conditions: the thiolated single-stranded DNA is one segment of any sequence which is non-complementary and non-homologous to a target sequence; the Tm value is not less than 37.7° C.; and at least one end contains a thiolalkyl group SH—C6H12—.