Patent ID: 10621704

Abstract:
The herein invention discloses and claims a quantitative image restoration in bright field (QRBF) method that faithfully recovers shape and enables quantify size of individual unstained samples. The QRBF method restores out-of-focus BF images of unstained samples by applying deconvolution, which enhances contrast and allows for quantitative analysis. To perform deconvolution our procedure uses a point spread function modeled from theory. Image deconvolution can be performed even from a single input image in two dimensions (2D-QRBF), and quantitative information such as size and shape of samples can be determined from restored 2D-QRBF images. Application of 2D-QRBF in a high-throughput screening process to assess shape changes in cells during hyperosmotic shock shows that the described digital restoration approach is suitable for quantitative analysis of unstained BF images in high-throughput image cytometry.