Patent ID: 10144976

Abstract:
A method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance and HIV tropism includes generating a plurality of cDNA species from a plurality of RNA molecules in an HIV sample population, amplifying a plurality of first amplicons and second amplicons from the cDNA species, wherein the first amplicons are amplified using first pairs of primers that amplify a HIV genomic region of the cDNA species encoding the Gag proteins p2, p7, p1 and p6, and the protease, reverse transcriptase, and integrase enzymes and the second amplicons are amplified using second pairs of primers that amplify a HIV genomic encoding region of the cDNA species encoding the env-C2V3 region; determining the nucleic acid sequence compositions of the amplified first amplicons second amplicons; identifying variants in the determined sequence by comparing the determined nucleic sequence to a guide sequence; and correlating the determined variants with variants of HIV drug resistance and HIV tropism.