Patent ID: 11274163

Abstract:
A method for creating an antibody ionic liquid, comprising: providing aqueous anti-A, anti-B, or anti-Rh antibodies; cationizing the aqueous antibodies by the addition of stoichiometric amounts of an excess of a positively-charged crosslinker in the presence of a coupling reagent; titrating the cationized antibodies with a counter anionic polymer to create at least one antibody cation/anion pair in aqueous solution until the antibody cation/anion pair solution becomes negative by zeta potential measurement. The method may further comprise lyophilizing the antibody cation/anion pair solution to remove all of the water, forming a lyophilized solid; and heating the lyophilized solid to generate a protein ionic liquid. Cationized antibodies may be purified of excess coupling reagents by dialysis in water by a membrane, and the antibody cation/anion solution may be dialyzed to remove excess anionic polymer with a similar membrane.