Patent ID: 11965889

Abstract:
The present invention relates to artificially synthesized modified mono-methylated lysines and modified mono-methylated lysine derivatized polypeptides. The present invention also relates to production of a completely new antibody by using this modified mono-methylated lysine and modified mono-methylated lysine derivatized polypeptide as an antigen, this antibody can be used in recognizing and enriching the modified polypeptide after the lysine mono-methylated polypeptide being derivatized in vitro. By using this antibody, it is capable of detecting whether a mono-methylation modification is present in amino acids on the polypeptide sequence, the present invention also relates to a preparation method of said antibody. Moreover, the present invention also relates to a method for identifying and quantifying the lysine mono-methylated modified substrate in cell or tissue; more specifically, it belongs to identifying and quantifying the lysine mono-methylated modified substrate in cell or tissue by using proteomics approach of affinity enrichment for specific antibody and mass spectrometry.