Patent ID: 10527527

Abstract:
A papaver from rhoeas' cell and tissue stain is formulated incorporating a new one bioflavonoid which specifically stains the nucleus for microscopic evaluation in histopathology, microbiology and cytology. It appears to be an alternative to hematoxylin for routine usage. Biochemical name of this compound is hydroxy-7-methoxy-2-(4-methoxy-3-(((2R,3R,4S,5S,6R)-3.4.5-trihydroxy-6-((((2R,3R,4R,5R,6S)-3.4.5-trihydroxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)methyl)tetrahydro-2H-pyran-2- yl)oxy)phenyl)-4H-chromen-4-one. NMR analysis shows the biochemical structure of molecule is a bioflavonoid. (FIG. 4,5) Molecule within Papaver rhoeas along with the synergistic and other molecules penetrates the biologic and nonbiologic samples. Combining with the other synergistic mechanisms the stain formula is prepared. The amount and type of Mordant and pH are the parameters that affect the quality and timing of the staining results.