Patent ID: 11618729

Abstract:
A method of preparing bilirubin IXα from biliverdin IXα diester, the method including: 1) adding sodium hydroxide dissolved in a first methanol solution to biliverdin IXα diester dissolved in a second methanol solution, to yield a first mixture; adding water to the first mixture to hydrolyze biliverdin IXα diester; adding an acid to the first mixture to adjust the pH value thereof; removing a first solvent of the first mixture through rotary evaporation, and removing an inorganic salt through rinsing, and vacuum drying, to yield biliverdin IXα; and 2) dissolving the biliverdin IXα in an alcoholic solution, and adding a radical scavenger and borohydride to the alcoholic solution, to yield a second mixture; adding a ketone to the second mixture to decompose excess borohydride; adding the acid to the second mixture to adjust the pH value thereof; and removing a second solvent of the second mixture through rotary evaporation.