Patent ID: 10889824

Abstract:
A method for genetic transformation of edible mushrooms is provided relating to the technical field of genetic transformation of Agaricus bisporus, enoki mushroom and shiitake. The disclosed method for genetic transformation of Agaricus bisporus includes: inoculating Agaricus bisporus liquid mycelia into a foxtail-millet-grain culture medium, and pre-culturing them at 20-25° C. until Agaricus bisporus mycelia grow on surfaces of foxtail millet grains. This method uses the foxtail millet grains as an attachment matrix, and during the pre-culturing and co-culturing, the culture substrate is shaken up every day. The method for genetic transformation of enoki mushroom or shiitake includes: inoculation of enoki mushroom mycelia or shiitake mycelia, activated culturing of agrobacterium, and agrobacterium infecting a foxtail millet grain-enoki mushroom mycelium matrix or a foxtail millet grain-shiitake mycelium matrix.