Patent ID: 10022402

Abstract:
A method of preparing an allogenic dendritic stem cell vaccine for treating ovarian cancers is disclosed. The method comprises, (a) collecting blood sample from an allogenic donor in a sterile collection bag, (b) separating viable mesenchymal stem cells inoculums by centrifuging the blood sample, suspending said mesenchymal stem cells inoculum in proliferation medium, plating the cells in a flask and incubated, and collecting cells adherent to the flask and further harvesting and expanding said adherent cells, (c) preparing myeloid dendritic cells by isolating, harvesting and culturing said blood sample source using myeloid dendritic isolation kit, (d) mixing the adherent cells obtained. Finally, (e) the composition of the adherent cells obtained in step (d) to the myeloid dendritic cells obtained in step (c) at a ratio of 90:10 and printing the cellular vectors with patient tumor derived heterogeneous progenitors.