Patent ID: 11821014

Abstract:
An expression vector that includes a polynucleotide having a heterologous regulatory element operably linked to a polynucleotide sequence derived from Solanum tuberosum and encoding a xylosyltransferase capable of glycosylating gentisic acid to produce gentisic acid 5-O-β-D xylopyranoside, a transcription template having such a polynucleotide and adapted for in vitro transcription in a cell-free system, a method for producing gentisic acid 5-O-β-D xylopyranoside by culturing a recombinant host cell containing such an expression vector under conditions in which the cell expresses the xylosyltransferase from the polynucleotide, and a method for producing gentisic acid 5-O-β-D xylopyranoside by contacting a composition including gentisic acid and UDP-xylose with a recombinant xylosyltransferase. The recombinant host cell containing such an expression vector can be a bacterial cell, a plant cell, or a fungal cell, an animal cell, or a multicellular organism such as a plant.