Patent ID: 11286475

Abstract:
The disclosure relates to a tyrosol-producing recombinant Escherichia coli and a construction method and application thereof and belongs to the technical field of bioengineering. The Escherichia coli undergoes heterologous expression of a codon-optimized Saccharomyces cerevisiae pyruvate decarboxylase gene ARO10*. According to the recombinant Escherichia coli, five sites of a lacI site, a trpE site, a pabB site, a pabA site and a pykF site of an Escherichia coli genome are deleted, and at the same time, the ARO10* gene is integrated at each site of the five sites to obtain a strain containing multiple copies of the ARO10* gene. On the basis of the above recombinant strain, the ARO10* gene is randomly integrated at multiple sites, and it is found that a strain with high-yield production of tyrosol can be obtained by inserting the ARO10* gene at a yccX site. Fermentation using this strain does not require inducers or antibiotics. After fermentation is carried out for 48 hours, the yield of tyrosol can reach 32.3 mM.