Patent ID: 11821005

Abstract:
An umbilical cord mesenchymal stem cells (MSCs) and culture method and application thereof, which comprises an MSCs culture step comprising the following substeps: primary culture: culturing Wharton's jelly of an umbilical cord in a serum-free medium under a hypoxic condition; subculture: collecting P1 primary cells to prepare a single cell suspension which is centrifuged to obtain a cell pellet; adding a serum-free medium to the cell pellet, culturing under a hypoxic condition until passage, and continuously culturing up to P2-P3; adding a ligustrazine hydrochloride and a Shenmai injection during each subculture, digesting cells grown to a predetermined fusion degree, and collecting the cells cultured to P6; phenotypic test: testing the phenotype of the collected cells for later use. An MSCs preparation cultured by the method reduces easy aggregation of stem cells, thus avoiding cell adhesion, rouleau formation of red cells and cell cluster embolism after intravenous infusion into human bodies.