Patent ID: 11774440

Abstract:
Disclosed is a method for detecting chemotaxis of neutrophil, including: mixing a blood sample and an equal volume of glucose, mixing, and standing; taking a supernatant of the blood sample after standing into a centrifuge tube, and centrifuging; adding 1× calcium and magnesium ion-free HBSS into a centrifuged blood sample to re-suspend cell aggregates at bottom; uniformly blowing and dispersing a re-suspended bottom cell aggregates, and slowly adding a polysucrose solution from the bottom of the centrifuge tube to perform density gradient centrifuging treatment; dividing a density gradient centrifuged solution into three layers, pipetting a clear liquid at an upper layer and a PBMC layer at a middle layer to obtain cell aggregates at a bottom layer; lysing erythrocytes in the cell aggregates, then successively adding 2× calcium and magnesium ion-free HBSS and 1× calcium and magnesium ion-free HBSS, mixing, and centrifuging again to obtain neutrophils.