Patent ID: 10881700

Abstract:
Human Immunodeficiency Virus (HIV) causes AIDS, a life-threatening disease characterized by immunosuppressive, opportunistic infections and malignancies. Although many drugs have been approved over the past decade as suitable for use in the treatment of individuals with HIV, the need for antiviral drugs of greater efficiency is still pressing. One valuable source for antiviral bioactivity has been proven to be the natural products of a wide range of plants. In this study, we investigated the anti-reverse transcriptase (RT)-HIV-1 potential activity of Avicennia marina (gray mangrove) collected from the Red Sea shore, Saudi Arabia. Metabolites from A. marina were extracted using organic solvents followed by solid phase extraction (SPE) and high-performance liquid chromatography (HPLC). Gas chromatography mass spectrometry (GC-MS) was applied to assess the active HPLC fractions and to establish a correlation between the fractions' chemical composition and biological activity. The chemical analyses revealed the existence of many polyphenol compounds. Polyphenol compounds have been proven to act as multi-target anti-HIV agents. Furthermore, imaging-based High-Content Screening (HCS) with a set of cellular staining was established to characterize mechanisms of activity and potential side-effects, such as toxicity and cell cycle arrest. In summary, we discovered and assessed for the first time anti-RT-HIV-1 activity for A. marina collected from Red Sea shore, Saudi Arabia. Our results suggest this plant is a promising candidate for the development of potential novel HIV-1 inhibitors.