Patent ID: 11421225

Abstract:
In an embodiment, a device for modulating intracellular gene expression, the device having a calcium actuator component and a transcription reprogramming component. In another embodiment, a method for modulating intracellular gene expression, where the method includes inducing a system having a calcium actuator component and a transcription reprogramming component with at least one of light and a chemical, causing an increase in Ca2+, and translocating the transcription reprogramming component from cytosol to the nucleus. In a further embodiment, a method for modulating gene intracellular expression, where the method includes inducing a system having an NIR-stimulable optogenetic platform with at least one of light and a chemical, where the NIR-stimulable optogenetic platform facilitates Ca2+ release and the NIR-stimulable optogenetic platform is LOV2-SOAR, causing an increase in Ca2+, and translocating a calcium-responsive dCas9 fusion construct from cytosol to the nucleus, where the calcium-responsive dCas9 fusion construct is NFAT1-460-dCas9-VP64.