Patent ID: 10379327

Abstract:
Provided is a scanning microscope including a pinhole array disk having a plurality of pinholes restricting a light flux of illumination light for irradiating a sample, the plurality of pinholes being disposed so as to form an array about a center axis; a rotational driving unit rotating the pinhole array disk about the center axis; an objective lens irradiating the sample with the illumination light that has passed through the pinholes and collecting fluorescence from the sample to cause the fluorescence to enter the pinholes; a camera acquiring an image of the sample by repeatedly capturing the fluorescence that has passed through the pinholes; and a stimulation optical system irradiating the sample with stimulation light, wherein the system includes a stimulation-timing generating unit decreasing the intensity of the stimulation light during exposure periods of the camera and increasing the intensity of the stimulation light during periods between exposure periods.