Patent ID: 11788154

Abstract:
Provided is a method for identifying a racehorse including amplifying a target gene by a multiplex PCR using a microsatellite marker obtained by combining one or more sets selected from a first set consisting of AHT4, AHT5, ASB2, HMS3, HMS6, HMS7, HTG4, HTG10, VHL20, ASB17, ASB23, HMS1, LEX3, CA425, HMS2, HTG6, HTG7, LEX033, AMEL, HMS18, LEX27, SRY, and LEX020 and a second set consisting of HTG21, COR089, TKY279, TKY287, TKY294, TKY297, TKY301, TKY312, TKY321, TKY325, TKY333, TKY337, TKY341, TKY343, TKY344, TKY374, and TKY394; detecting alleles in the product amplified in the multiplex PCR amplification step and analyzing the sizes of the alleles using an electrophoresis apparatus to determine a genotype of the racehorse; and summarizing the sizes of the alleles analyzed using the electrophoresis apparatus according to the population and breed of racehorses to plot the number and a frequency distribution of the alleles based on the summarized results.