Patent ID: 11262570

Abstract:
Methods for imaging a sample using fluorescence microscopy, systems for imaging a sample using fluorescence microscopy, and illumination systems for fluorescence microscopes. In some examples, a method includes positioning a dual convex paraboloidal mirror enclosure around the sample. The dual convex paraboloidal mirror enclosure includes an upper paraboloidal mirror and a lower paraboloidal mirror oriented antiparallel to each other. An aperture is defined in the lower paraboloidal mirror, a hemispherical dome is mounted in the aperture, and the sample is surrounded by the hemispherical dome. The method includes directing excitation light onto the sample to form a primary image at an upper vertex of the upper paraboloidal mirror and a secondary image at a lower vertex of the lower paraboloidal minor. The method includes imaging the sample through a detection objective of a microscope.