Patent ID: 11649444

Abstract:
The present disclosure provides a Cas12i protein (e.g., non-naturally occurring, engineered) comprising an amino acid sequence having at least about 80% (e.g., at least about any of 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%) identity to the amino acid sequence as set forth in any one of SEQ ID NOs: 1-10 (preferably, SEQ ID NOs: 1-3 and 6, and more preferably, SEQ ID NO: 1). The present disclosure further provides an engineered, non-naturally occurring CRISPR-Cas12i system comprising: (1) any of the Cas12i proteins described herein or a polynucleotide encoding any of the Cas12i proteins described herein; and (2) a CRISPR RNA (crRNA) or a polynucleotide encoding the crRNA, wherein the crRNA comprises: (i) a spacer capable of hybridizing to a target sequence of a target DNA, and (ii) a Direct Repeat (DR) linked to the spacer and capable of guiding the binding of the Cas12i protein to the crRNA to form a CRISPR-Cas12i complex targeting the target sequence.