Patent ID: 11104906

Abstract:
The present invention provides a method for synthesizing NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH so as to supplement NAD+ lost due to thermal decomposition in the enzyme reaction system, and a transformant set for coenzyme production used in the method. That is, the present invention relates to a method for producing a coenzyme including performing synthesis of NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH by adding one or more thermostable enzymes required for a reaction for synthesizing NAD+ from nicotinamide, to the enzyme reaction system, and a transformant set for coenzyme production including one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing NAD+ or NADH from nicotinamide are introduced by using a non-thermostable microorganism as a host.