Patent ID: 11965010

Abstract:
Disclosed are a method for preparing a porcine-derived interferon-delta 5 (pIFN-δ5) and an application of the pIFN-δ5, where the method for preparing pIFN-δ5 includes the following steps: step S1, obtaining a DNA fragment containing pIFN-δ5 gene through reverse transcription-polymerase chain reaction (RT-PCR) amplification by using the total RNA of pretreated porcine small intestinal epithelial cells IPEC-J2; step S2, inserting the DNA fragment containing pIFN-δ5 gene into an exogenous expression vector to construct a recombinant expression vector for expressing the pIFN-δ5 gene; and step S3, introducing the recombinant expression vector into a suitable host cell, and driving the host cell to express the pIFN-δ5 gene to obtain the pIFN-δ5. The recombinant pIFN-δ5 protein is used to prepare drugs or preparations for inhibiting infection of porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine delta coronavirus (PDCoV) and porcine rotavirus (PRoV).