Patent ID: 10704039

Abstract:
Device and method for extraction of nucleic acids, comprising a hollow body, preferably a pipette tip, through which a liquid is passed, characterized in that a material with rough or structured surface is disposed in this hollow body in such a way that it can be circumcirculated by a liquid. After lysis of the sample and adjustment of necessary binding conditions for adsorption of the nucleic acids on the carrier material, the mixture, by means of pipetting processes, is repeatedly “pipetted along” the material for binding nucleic acids, introduced vertically in the pipette tip. The nucleic acids bind to the material. Thereupon washing buffers are likewise “pipetted along” the material for binding nucleic acids. Then a drying step is performed. Finally, the eluent is again repeatedly “pipetted along” the vertically disposed material for binding nucleic acids, and in the process the bound nucleic acid is detached. The nucleic acid is now available for necessary downstream application.