Patent ID: 10072307

Abstract:
An isolation and detection method for viruses from large quantities of water or other aqueous media. The technology employs charged resins to isolate viral particles from liquid sources such as water, milk, juices, and homogenized food. Anionic resin beads are added to a sample liquid and suspended within the liquid. While in suspension the anionic beads complex with negatively charged virus particles of other pathogens. The complexed beads are then removed from the liquid, effecting the isolation of the virus. Detection techniques can then be performed directly on the complexed beads without resorting to additional steps to elute the virus or other pathogen from the bead. The methodology can be employed for the detection of bacteriophage in an aqueous medium where the bacteriophage serves as a surrogate for the presence of a pathogen or other contaminant, such as an enteric virus.