Patent ID: 11952624

Abstract:
Methods and kits for sequencing a nucleic acid molecule are provided, which include utilizing four different compounds that are respectively derivatives of nucleotides A, (T/U), C and G, wherein the hydroxyl at the 3′-position of sugar of each of the four compounds is protected by a reversible protecting group. Each of the four compounds comprises a first linker, a second linker, and a terminal molecular label binding to or reactive to a receptor in a detectable group comprising a luminescence-activating molecule and the receptor, the luminescence-activating molecule capable of causing emission of fluorescence in the presence of a suitable substrate. The second linkers in the four compounds are different, and can be cleaved at different conditions. After incorporating one of the four compounds into a growing chain using the target nucleic acid molecule as a template, a series of reactions is performed to cleave the second linker and the first linker, and a series of detections are performed for fluorescent signals, thereby determining the identity of the nucleotide of the incorporated compound.