Maximilian II, Holy Roman Emperor
Born at the Bohemian court in Prague, Anna was the third of fifteen children of King Ferdinand I (1503–1564) from his marriage with the Jagiellonian princess Anna of Bohemia and Hungary (1503–1547). Her siblings included: Elizabeth, Queen of Poland, Maximilian II, Holy Roman Emperor, Ferdinand II, Archduke of Austria, Catherine, Queen of Poland, Eleanor, Duchess of Mantua, Barbara, Duchess of Ferrara, Charles II, Archduke of Austria and Johanna, Duchess of Tuscany.