Clostridium tetani
MenAfriVac is a freeze-dried vaccine of a polysaccharide from a type of "Neisseria meningitidis" called group A. The polysaccharide has been purified by affinity chromatography and bound to a carrier protein called tetanus toxoid. The TT is prepared by extraction by ammonium sulfate precipitation and the toxin is inactivated with formalin from cultures of "Clostridium tetani" grown in a modified Mueller-Hinton agar.