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The watershed features a number of native and introduced fish, many of which are game fish. A 2000 survey found American Eel, Pumpkinseed, and Tessellated darter to be the most abundant species in the North Branch. A 2008 survey found that North Branch tributary creeks hold large populations of Eastern blacknose dace and Longnose dace. Largemouth Bass, Fallfish, Common shiner, Common carp, Bluegill, Redbreast sunfish, Rock Bass, White sucker, and Banded killifish are also found throughout the North Branch and its tributaries.