Derby d'Italia
On 10 January 2007, it was confirmed by Ajax Technical Director Martin van Geel that Grygera would be leaving for Juventus on a Bosman transfer when his contract with Ajax expired in June 2007. He was supposed to transfer after the 2006 FIFA World Cup, but the "Calciopoli" scandal prevented him from doing so. He expressed his delight over the possibility of playing with one of his Czech compatriots, Pavel Nedvěd. Due to his versatility, he soon established himself in the Juventus starting lineup. In March, he scored and subsequently set up David Trezeguet's goal in a 2–0 win over Genoa, and also scored a last-gasp equalizer in the club's Derby d'Italia fixture against rivals Internazionale on 18 April 2009.