Técnico Universitario
Twelve teams competed in the 2010 Serie A season, ten of whom remained from the 2009 season. LDU Portoviejo and Técnico Universitario were relegated last season to the Serie B after accumulating the fewest points in the First and Second Stage aggregate table. They were replaced by Independiente José Terán and Universidad Católica, the 2009 Serie B winner and runner-up, respectively. This was Universidad Católica's 27th season in the Serie A, having last played in the league in 2008. This was Independiente José Terán's first season in the Serie A.