Ottokar I of Bohemia
Anna was probably born in Prague, Bohemia, the daughter of King Ottokar I of Bohemia and his second wife, Constance of Hungary. Her maternal grandparents were Béla III of Hungary and his first wife, Agnes of Antioch. Her paternal grandparents were King Vladislaus II of Bohemia and Judith of Thuringia. She was a sister of the Franciscan nun Agnes of Bohemia (1211–1282).