Técnico Universitario
Twelve teams competed in the 2012 Serie A season, ten of whom remained from the previous season. ESPOLI and Imbabura were relegated last season after accumulating the fewest points in the 2011 season aggregate table. They will be replaced by Técnico Universitario and Macará, the 2011 Serie B winner and runner-up, respectively. Both teams from Ambato are making returns to the Serie A. Macará is returning after one season while Técnico Universitario is returning after two.