تثبيت النيتروجين
"Azotobacter "can fix at least 10 μg of nitrogen per gram of glucose consumed. Nitrogen fixation requires molybdenum ions, but they can be partially or completely replaced by vanadium ions. If atmospheric nitrogen is not fixed, the source of nitrogen can alternatively be nitrates, ammonium ions, or amino acids. The optimal pH for the growth and nitrogen fixation is 7.0–7.5, but growth is sustained in the pH range from 4.8 to 8.5. "Azotobacter" can also grow mixotrophically, in a molecular nitrogen-free medium containing mannose; this growth mode is hydrogen-dependent. Hydrogen is available in the soil, thus this growth mode may occur in nature.