Francis, Duke of Guise
Mary was born at Bar-le-Duc, Lorraine, the eldest daughter of Claude of Lorraine, Duke of Guise, head of the House of Guise, and his wife Antoinette de Bourbon, herself the daughter of Francis, Count of Vendome, and Marie de Luxembourg. Among her 11 siblings were Francis, Duke of Guise; Claude, Duke of Aumale; Charles, Cardinal of Lorraine; and Louis I, Cardinal of Guise.