African clawed frog
To prove the essential role of these gap junctions in embryological development, Warner conducted experiments during the 1980s to block these junctions and observe the resulting effects. Using the 8-cell-embryos of the African clawed frog, "Xenopus", she used antibody injections to block the channels in the connections of the gap junctions. After injecting the embryos with a specific antibody, which was said to have blocked the channels of gap junctions, Warner confirmed the blockage of the channels by injecting dyes into the cells in addition to confirming the lack of electrical coupling that she observed in earlier experiments.