TATA box
The conformational changes induced by TBP binding to the TATA box allows for additional transcription factors and RNA polymerase II to bind to the promoter region. TFIID first binds to the TATA box, facilitated by TFIIA binding to the upstream part of the TFIID complex. TFIIB then binds to the TFIID-TFIIA-DNA complex through interactions both upstream and downstream of the TATA box. RNA polymerase II is then recruited to this multi-protein complex with the help of TFIIF. Additional transcription factors then bind, first TFIIE and then TFIIH. This completes the assembly of the preinitiation complex for eukaryotic transcription. Generally, the TATA box is found at RNA polymerase II promoter regions, although some "in vitro" studies have demonstrated that RNA polymerase III can recognize TATA sequences.