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Justiniani entered the Jesuit noviciate at Rome in 1579 and later taught rhetoric in the Roman College, and then theology at Toulouse, Messina, and Rome. For more than twenty years, he served as head of the Roman College and regens of the Sacra Poenitentiaria (Apostolic Penitentiary). He also filled the post of Chief Preacher to the Pope. Pope Clement VIII appointed him theologian to Cardinal Thomas Cajetan, during his legation in Poland. Justiniani died at Rome in 1622.