Maximilian II, Holy Roman Emperor
The son of Alfonso and Lucrezia Borgia, Ercole d'Este II (1508–1559), married Renée of France, daughter of Louis XII of France. His son Alfonso II first married Lucrezia, daughter of grand-duke Cosimo I of Tuscany. After she died, he married Barbara, the sister of Maximilian II, Holy Roman Emperor (1527–1576). His third wife, Margherita Gonzaga, was daughter of the duke of Mantua.