Tianqi Emperor
The Tianqi Emperor died heirless on 30 September 1627 due to his only son having died in the Wanggongchang Explosion and was succeeded by his fifth and sole surviving brother, Zhu Youjian, because he had no sons to succeed him. Zhu Youjian was enthroned as the Chongzhen Emperor. As both the Tianqi Emperor's daughters died early too, it seems that there are no natural heirs from the emperor left alive.